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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (51)

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Types of Cytogenetic Abnormalities in the Apical Root Meristem of Elodea canadensis from the Yenisei River Areas with Different Types of Anthropogenic Pollution / M. Y. Medvedeva, A. Y. Bolsunovsky // Russ. J. Genet. Appl. Res. - 2018. - Vol. 8, Is. 2. - P115-123, DOI 10.1134/S2079059718020089 . - ISSN 2079-0597
Кл.слова (ненормированные):
anthropogenic contamination of bottom sediments -- Elodea canadensis -- genotoxicity -- mitotic abnormalities -- Yenisei River
Аннотация: The change in the spectrum of chromosomal abnormalities in the apical root meristem of Elodea canadensis Michx. (elodea) growing in areas with different types of anthropogenic (radioactive and chemical) pollution of the bottom sediments of the Yenisei River is shown. In the samples of elodea collected in the zone of radioactive contamination of the river by discharges of the Rosatom Mining and Chemical Combine (MCC, Zheleznogorsk), the predominant type of abnormalities consist of chromosomal aberrations (bridges, stickiness, chromosomal fragmentation), as well as multiple abnormalities in one cell. The elodea samples from the areas of the Yenisei River with nonradioactive (chemical) contamination of bottom sediments by the enterprises of the city of Krasnoyarsk are predominantly characterized by abnormalities associated with the disturbance of the mitotic spindle of cell division. © 2018, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Medvedeva, M. Y.; Bolsunovsky, A. Y.

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Trends in microevolution of microbial populations in open systems / A. V. Brilkov [et al.] // Doklady Biochemistry and Biophysics. - 2005. - Vol. 404, Is. 1-6. - P349-352, DOI 10.1007/s10628-005-0111-x . - ISSN 1607-6729
Кл.слова (ненормированные):
article -- bacterial phenomena and functions -- bacterium -- biological model -- culture technique -- Escherichia coli -- evolution -- genetics -- growth, development and aging -- mathematics -- methodology -- mutation -- nanotechnology -- pH -- physiology -- population dynamics -- time -- Bacteria -- Bacterial Physiology -- Cell Culture Techniques -- Escherichia coli -- Evolution -- Hydrogen-Ion Concentration -- Mathematics -- Models, Biological -- Mutation -- Nanotechnology -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Brilkov, A.V.; Loginov, I.A.; Morozova, E.V.; Pechurkin, N.S.

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The method to compare nucleotide sequences based on the minimum entropy principle [Text] / M. G. Sadovsky // Bull. Math. Biol. - 2003. - Vol. 65, Is. 2. - P. 309-322, DOI 10.1016/S0092-8240(02)00107-6. - Cited References: 20 . - ISSN 0092-8240

РУБ Biology + Mathematical & Computational Biology
Рубрики:
GENOME
Аннотация: A new method to compare two (or several) symbol sequences is developed. The method is based on the comparison of the frequencies of the small fragments of the compared sequences; it requires neither string editing, nor other transformations of the compared objects. The comparison is executed through a calculation of the specific entropy of a frequency dictionary against the special dictionary called the hybrid one; this latter is the statistical ancestor of the group of sequences under comparison. Some applications of the developed method in the fields of genetics and bioinformatics are discussed. (C) 2003 Society for Mathematical Biology. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Akad Gorodok, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sadovsky, M.G.

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The comparative redundancy of genes of various organisms and viruses / A. N. Gorban [и др.] // Genetika. - 1993. - Vol. 29, Is. 9. - P. 1413-1419 . - ISSN 0016-6758
Кл.слова (ненормированные):
article -- dna content -- evolution -- genome -- nonhuman -- restriction mapping -- virus -- amino acid sequence -- comparative study -- gene frequency -- human -- molecular genetics -- nucleotide sequence -- restriction mapping -- virus gene -- Amino Acid Sequence -- Base Sequence -- Comparative Study -- English Abstract -- Gene Frequency -- Genes, Viral -- Human -- Molecular Sequence Data -- Restriction Mapping

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gorban, A.N.; Mirkes, E.M.; Popova, T.G.; Sadovsky, M.G.

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Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms / A. N. Boyandin [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1763-1768, DOI 10.1016/S0273-1177(03)00118-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Cells -- Escherichia coli -- Salts -- Recombinant plamid -- Space research -- ampicillin -- chloride -- inorganic salt -- magnesium -- mineral -- potassium -- sodium -- sulfate -- genetically modified organism -- article -- bacterial count -- chemoluminescence -- culture medium -- drug effect -- Escherichia coli -- genetics -- growth, development and aging -- microbiology -- microclimate -- penicillin resistance -- plasmid -- Russian Federation -- transgenic organism -- Ampicillin -- Ampicillin Resistance -- Chemiluminescent Measurements -- Chlorides -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Escherichia coli -- Magnesium -- Minerals -- Organisms, Genetically Modified -- Plasmids -- Potassium -- Russia -- Salts -- Sodium -- Sulfates -- Water Microbiology
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap'Lux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g 1-1) taken from Lake Shira (Khakasia, Russia). The survivof the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 ?g/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 ?g/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 ?g/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Popova, L.Yu.; Pechurkin, N.S.

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Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions / L. Yu. Popova [и др.] // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 796-803 . - ISSN 0026-3656
Кл.слова (ненормированные):
Antibiotic resistance -- Bioluminescence interferon -- Recombinant plasmids -- Transgenic microorganisms -- article -- Bacillus subtilis -- culture medium -- Escherichia coli -- genetic engineering -- genetic recombination -- genetics -- R factor -- Bacillus subtilis -- Culture Media -- Escherichia coli -- Genetic Engineering -- R Factors -- Recombination, Genetic
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Maksimova, E.E.; Lobova, T.I.; Kargatova, T.V.; Boyandin, A.N.; Krylova, T.Yu.; Pechurkin, N.S.

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Species composition of winter bacterioplankton in two Siberian ponds determined by the 16s rRNA sequence analysis. / M. Y. Trusova, M. I. Gladyshev // Doklady Biological Sciences. - 2002. - Vol. 382. - P51-54 . - ISSN 0012-4966
Кл.слова (ненормированные):
bacterial RNA -- RNA 16S -- animal -- article -- bacterium -- classification -- ecosystem -- genetics -- microbiology -- molecular cloning -- molecular genetics -- plankton -- Russian Federation -- season -- species difference -- Animals -- Bacteria -- Cloning, Molecular -- Ecosystem -- Molecular Sequence Data -- Plankton -- RNA, Bacterial -- RNA, Ribosomal, 16S -- Seasons -- Siberia -- Species Specificity -- Water Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Y.; Gladyshev, M.I.

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Species composition of winter bacterioplankton in blooming and nonblooming reservoirs as determined by 16S rRNA sequences / M. Yu. Trusova, M. I. Gladyshev // Doklady Biological Sciences. - 2005. - Vol. 405, Is. 1-6. - P443-445, DOI 10.1007/s10630-005-0160-4 . - ISSN 0012-4966
Кл.слова (ненормированные):
fresh water -- RNA 16S -- article -- bacterium -- classification -- genetic variability -- genetics -- isolation and purification -- microbiology -- molecular genetics -- phylogeny -- plankton -- Russian Federation -- season -- species difference -- Bacteria -- Fresh Water -- Molecular Sequence Data -- Phylogeny -- Plankton -- RNA, Ribosomal, 16S -- Seasons -- Siberia -- Species Specificity -- Variation (Genetics) -- Water Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Yu.; Gladyshev, M.I.

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Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia / Y.-T. Wu, P.-W. Chiang, K. Tandon [et al.] // Microb. Genomics. - 2021. - Vol. 7, Is. 12. - Ст. 000712, DOI 10.1099/mgen.0.000712 . - ISSN 2057-5858
Кл.слова (ненормированные):
Flow cytometry -- Lake Shunet -- Purple sulfur bacteria -- Single-cell genomics -- genomic DNA -- RNA 16S -- Article -- bioinformatics -- carbon metabolism -- Enterobacter -- fluorescence activated cell sorting -- gene amplification -- gene ontology -- high throughput sequencing -- metagenomics -- microbial community -- microbial diversity -- molecular genetics -- nitrogen metabolism -- nonhuman -- nucleotide sequence -- phylogenetic tree -- phylogeny -- polymerase chain reaction -- Sanger sequencing -- Thiocapsa
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.

Scopus
Держатели документа:
Department of Forestry, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan
Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Wu, Y. -T.; Chiang, P. -W.; Tandon, K.; Rogozin, D. Y.; Degermendzhy, A. G.; Tang, S. -L.

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10.

Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275 . - ISSN 0026-3656
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid -- article -- Bacillus subtilis -- culture medium -- genetic recombination -- genetics -- plasmid -- Bacillus subtilis -- Culture Media -- Plasmids -- Recombination, Genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

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11.

Population dynamics of transgenic strain Escherichia coli Z905/pPHL7 in freshwater and saline lake water microcosms with differing microbial community structures / L. Yu. Popova [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1573-1578, DOI 10.1016/j.asr.2005.01.037 . - ISSN 0273-1177
Кл.слова (ненормированные):
Microcosm -- Plasmid -- Population dynamics -- Transgenic microorganism -- Bacteria -- Genes -- Microorganisms -- Saline water -- Microcosms -- Plasmid -- Population dynamics -- Transgenic microorganism -- Escherichia coli -- brine -- fresh water -- inorganic salt -- bacterial gene -- conference paper -- ecosystem -- Escherichia coli -- gene expression regulation -- genetics -- growth, development and aging -- microbiology -- Micrococcus -- penicillin resistance -- plasmid -- population dynamics -- transgenic organism -- Ampicillin Resistance -- Ecosystem -- Environmental Microbiology -- Escherichia coli -- Fresh Water -- Gene Expression Regulation, Bacterial -- Genes, Bacterial -- Micrococcus -- Organisms, Genetically Modified -- Plasmids -- Population Dynamics -- Salts -- Water Microbiology
Аннотация: Populations of Escherichia coli Z905/pPHL7, a transgenic microorganism, were heterogenic in the expression of plasmid genes when adapting to the conditions of water microcosms of various mineralization levels and structure of microbial community. This TM has formed two subpopulations (ampicillin- resistant and ampicillin-sensitive) in every microcosm. Irrespective of mineralization level of a microcosm, when E. coli Z905/pPHL7 alone was introduced, the ampicillin-resistant subpopulation prevailed, while introduction of the TM together with indigenous bacteria led to the dominance of the ampicillin-sensitive subpopulation. A high level of lux gene expression maintained longer in the freshwater microcosms than in sterile saline lake water microcosms. A horizontal gene transfer has been revealed between the jointly introduced TM and Micrococcus sp. 9/pSH1 in microcosms with the Lake Shira sterile water. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Controlled Heterotroph Biosynthesis, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation
Siberian Branch, Institute of Computational Modeling, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Kargatova, T.V.; Ganusova, E.E.; Lobova, T.I.; Boyandin, A.N.; Mogilnaya, O.A.; Pechurkin, N.S.

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12.

Population dynamics of transgenic microorganisms in the different microecosystem conditions / L. Yu. Popova [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1571-1579, DOI 10.1016/S0273-1177(01)00248-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Ecosystems -- Escherichia coli -- Genes -- Interferons -- Luminescence -- Vaccines -- Microcosms -- Microorganisms -- fresh water -- microorganism -- adaptation -- article -- Bacillus subtilis -- comparative study -- ecosystem -- Escherichia coli -- gene expression -- genetics -- growth, development and aging -- hazardous waste -- microbiology -- Micrococcus -- osmotic pressure -- plasmid -- population dynamics -- risk assessment -- transgenic organism -- Adaptation, Physiological -- Bacillus subtilis -- Containment of Biohazards -- Ecosystem -- Escherichia coli -- Fresh Water -- Gene Expression -- Micrococcus -- Organisms, Genetically Modified -- Osmotic Pressure -- Plasmids -- Population Dynamics -- Risk Assessment -- Water Microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.

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13.

Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 6. - С. 788-794 . - ISSN 0026-3656
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator -- bacterial RNA -- glucose -- hydrogen -- hydroxybutyric acid -- polymer -- RNA 16S -- article -- culture medium -- genetics -- growth, development and aging -- metabolism -- mutation -- oxidation reduction reaction -- physiology -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Glucose -- Hydrogen -- Hydroxybutyrates -- Mutation -- Oxidation-Reduction -- Polymers -- RNA, Bacterial -- RNA, Ribosomal, 16S
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO 2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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14.

Phylogenetic diversity of winter bacterioplankton of eutrophic Siberian reservoirs as revealed by 16S rRNA gene sequences / M. Yu. Trusova, M. I. Gladyshev // Microbial Ecology. - 2002. - Vol. 44, Is. 3. - P252-259, DOI 10.1007/s00248-002-2020-1 . - ISSN 0095-3628
Кл.слова (ненормированные):
bacterioplankton -- community composition -- community dynamics -- genetic variation -- phylogenetics -- reservoir -- winter -- Russian Federation -- Actinobacteria -- Bacteria (microorganisms) -- Bacteroides -- Betaproteobacteria -- Cyanobacteria -- Cytophaga -- Flavobacterium -- Prokaryota -- Proteobacteria -- bacterial DNA -- fresh water -- RNA 16S -- animal -- article -- bacterium -- chemistry -- DNA sequence -- genetic variability -- genetics -- microbiology -- molecular genetics -- phylogeny -- plankton -- Russian Federation -- season -- Animals -- Bacteria -- DNA, Bacterial -- Fresh Water -- Molecular Sequence Data -- Phylogeny -- Plankton -- RNA, Ribosomal, 16S -- Seasons -- Sequence Analysis, DNA -- Siberia -- Variation (Genetics)
Аннотация: Using 16S rRNA gene sequence analyses we investigated the bacterial diversity of winter bacterioplankton of two eutrophic Siberian reservoirs. These reservoirs show similarity in phytoplankton community composition in spring and autumn but tend to differ in summer in exhibiting cyanobacterial bloom. Forty-eight unique partial 16S RNA gene sequences retrieved from two libraries were mostly affiliated with the class Actinobacteria, ? subdivision of the class Proteobacteria, and the phylum Cytophaga-Flavobacterium-Bacteroides. The clone library of the pond exhibiting summer cyanobacterial bloom showed more diversity in sequence composition. A significant number of bacterial 16S rRNA gene clones were closely related to freshwater bacteria previously found in different aquatic ecosystems. This finding confirms the assumption that some bacterial clades are globally distributed.

Scopus
Держатели документа:
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Yu.; Gladyshev, M.I.

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15.

Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms / T. V. Kargatova [и др.] // Mikrobiologiya. - 1997. - Vol. 66, Is. 1. - С. 101-106 . - ISSN 0026-3656
Кл.слова (ненормированные):
article -- Escherichia coli -- genetic recombination -- genetics -- luminescence -- microbiology -- penicillin resistance -- phenotype -- plasmid -- Ampicillin Resistance -- Escherichia coli -- Luminescent Measurements -- Phenotype -- Plasmids -- Recombination, Genetic -- Water Microbiology
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.

Scopus
Держатели документа:
Institute uf Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.; Bril'Kov, A.V.; Pechurkin, N.S.

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16.

On the problem of genome redundancy in viruses and prokaryotes [Text] / M. G. Sadovsky // Russ. J. Genet. - 2002. - Vol. 38, Is. 5. - P. 575-581, DOI 10.1023/A:1015503617023. - Cited References: 10 . - ISSN 1022-7954

РУБ Genetics & Heredity

Аннотация: A specific index of nucleotide sequence redundancy, the specific restriction length of a finite frequency dictionary, was determined for a complete set of genes in some viral genomes and a genome of a bacterium, Bacillus subtilis. The distribution of the gene number over the specific restriction length was shown to be bimodal for viral genomes and unimodal for the Bac. subtilis genome. These results agree with earlier data.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sadovsky, M.G.

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17.

Nucleotide sequence of part of Photobacterium leiognathi lux region / B. A. Illarionov [et al.] // Nucleic Acids Research. - 1988. - Vol. 16, Is. 20. - P9855, DOI 10.1093/nar/16.20.9855 . - ISSN 0305-1048
Кл.слова (ненормированные):
bacterial protein -- luciferase -- article -- bacterial gene -- genetics -- molecular genetics -- nucleotide sequence -- Photobacterium -- Bacterial Proteins -- Base Sequence -- Genes, Bacterial -- Luciferase -- Molecular Sequence Data -- Photobacterium

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Krasnoyarsk, Russian Federation
Institute of Clinical and Experimental Medicine, Novosibirsk, Russian Federation
Novosibirsk Institute of Bioorganic Chemistry, 630090, Novosibirsk, Lavrentjev prospect 8, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Illarionov, B.A.; Protopopova, M.V.; Karginov, V.A.; Mertvetsov, N.P.; Gitelson, J.I.

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18.

Modelling of genetically engineered microorganisms introduction in closed artificial microcosms / N. S. Pechurkin [et al.] // Advances in Space Research. - 1999. - Vol. 24, Is. 3. - P335-341, DOI 10.1016/S0273-1177(99)00320-8 . - ISSN 0273-1177
Кл.слова (ненормированные):
aquatic environment -- artificial ecosystem -- ecological modeling -- genetically modified organism -- alga -- animal -- article -- bacterial count -- bacterial gene -- biological model -- biomass -- Escherichia coli -- feasibility study -- genetic engineering -- genetics -- growth, development and aging -- microbiology -- microclimate -- Photobacterium -- plasmid -- protozoon -- time -- yeast -- Algae -- Animals -- Biomass -- Colony Count, Microbial -- Ecological Systems, Closed -- Escherichia coli -- Feasibility Studies -- Genes, Bacterial -- Genetic Engineering -- Models, Biological -- Photobacterium -- Plasmids -- Protozoa -- Time Factors -- Water Microbiology -- Yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Brilkov, A.V.; Ganusov, V.V.; Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.

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19.

Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat / V. V. Ganusov, A. V. Bril'kov, N. S. Pechurkin // Biofizika. - 2000. - Vol. 45, Is. 5. - С. 908-914 . - ISSN 0006-3029
Кл.слова (ненормированные):
article -- bioreactor -- Escherichia coli -- fermentation -- genetics -- growth, development and aging -- Photobacterium -- plasmid -- theoretical model -- Bioreactors -- Escherichia coli -- Fermentation -- Models, Theoretical -- Photobacterium -- Plasmids
Аннотация: A structural approach to studying the regularities of the population dynamics of unstable recombinant bacterial strains in a chemostat was elaborated. The approach is based on the mathematical modeling of cell distribution in a population with different numbers of plasmid copies. The effect of decreased selective preference of plasmidless variants of the recombinant strain in the chemostat, which is related to a decrease in the number of plasmid copies in cells upon long-term incubation was analyzed. It is shown that the time of half-elimination of plasmids from the bacterial population in the steady state in the chemostat T1/2 does not depend on the maximum number of plasmid copies in cells N but is determined only by the mean time of generation g and the probability of the loss of one plasmid copy tau. The dependence of the preference of bacterial plasmidless variants on the efficiency of expression of genes cloned into plasmids in chemostat was analyzed using the recombinant strain E. coli Z905, whose plasmids pPHL-7 contain cloned genes for the luminescence system of marine luminescing bacteria Photobacterium leiognathi.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ganusov, V.V.; Bril'kov, A.V.; Pechurkin, N.S.

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20.

Luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters / A. N. Boyandin [et al.] // Doklady Biochemistry and Biophysics. - 2007. - Vol. 416, Is. 1-6. - P248-251, DOI 10.1134/S1607672907050067 . - ISSN 1607-6729
Кл.слова (ненормированные):
photoprotein -- polyester -- polyhydroxyalkanoic acid -- article -- bacterium -- chemistry -- chemoluminescence -- classification -- genetic procedures -- genetics -- metabolism -- Photobacterium -- phylogeny -- time -- Bacteria -- Biosensing Techniques -- Chemiluminescent Measurements -- Luminescent Proteins -- Photobacterium -- Phylogeny -- Polyesters -- Polyhydroxyalkanoates -- Time Factors -- Bacteria (microorganisms)

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Kalacheva, G.S.; Rodicheva, E.K.; Volova, T.G.

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