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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodionova N.S., Bondar V.S., Petushkov V.N.
Заглавие : Ca(2+)-activator of the luminescence system of the earthworms Henlea sp., (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady. Biochemistry and biophysics. - 2002. - Vol. 386. - С. 260-263. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): calcium--divalent cation--edetic acid--luciferase--luciferin--metal--animal--annelid worm--article--chemistry--dose response--enzymology--genetics--kinetics--luminescence--metabolism--animals--calcium--cations, divalent--dose-response relationship, drug--edetic acid--firefly luciferin--kinetics--luciferases--luminescent measurements--metals--oligochaeta
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : Comparison of real frequencies of strings vs. the expected ones reveals the information capacity of macromoleculae
Колич.характеристики :16 с
Место публикации : J. Biol. Phys.: SPRINGER, 2003. - Vol. 29, Is. 1. - P23-38. - ISSN 0092-0606, DOI 10.1023/A:1022554613105
Примечания : Cited References: 45
Предметные рубрики: DNA-SEQUENCE
ENTROPY
REDUNDANCY
Ключевые слова (''Своб.индексиров.''): dictionary--entropy--information capacity--markov model--ordered sequence--random sequence--reconstructed dictionary--specific entropy
Аннотация: The information capacity of nucleotide sequences is defined through the calculation of specific entropy of their frequency dictionary. The specific entropy of the frequency dictionary is calculated against the reconstructed dictionary; this latter bears the most probable continuations of the shorter strings. This developed measure allows to distinguish the sequences both from the randons ones, and from those with high level of (rather simple) order. Some implications of the developed methodology in the fields of genetics, bioinformatics, and molecular biology are discussed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : Comparison of symbol sequences: No editing, no alignment
Колич.характеристики :18 с
Место публикации : Open Syst. Inf. Dyn.: KLUWER ACADEMIC PUBL, 2002. - Vol. 9, Is. 1. - P19-36. - ISSN 1230-1612, DOI 10.1023/A:1014278811727
Примечания : Cited References: 12
Предметные рубрики: MULTIPLE
Аннотация: The new method to compare two (or several) symbol sequences is developed. The method is based on the comparison of the frequencies of the small fragments of the compared sequences; it requires no string editing or other transformations of the compared objects. The comparison is provided through a calculation of the specific entropy of a frequency dictionary against the special dictionary called the hybrid one; the latter is the statistical ancestor of the group of sequences to be compared. Some applications of the method developed to genetics, bioinformatics, and linguistics axe discussed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lesniak D.V., Popova L.I.
Заглавие : Conflict: induction-inhibition of transgene bacteria luminescence in studying expression of lux-genes
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 6. - P1059-1063. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): naphthalene derivative--salicylic acid derivative--article--bacterial gene--chemistry--chemoluminescence--culture medium--escherichia coli--gene expression regulation--genetics--metabolism--pseudomonas fluorescens--transgene--chemiluminescent measurements--culture media--escherichia coli--gene expression regulation, bacterial--genes, bacterial--naphthalenes--pseudomonas fluorescens--salicylates--transgenes
Аннотация: The relationship between the induction of the luminescent operon of lux-genes fused with the naphthalene and salicylate degradation genes and the inhibition of light emission caused by these compounds was studied. The quantitative correlations between these processes manifest themselves in the fact that light intensity linearly increased in a narrow concentration range of the inductor and then decreased due to the inhibition of the luminescence reaction itself, which is not related to the regulation of expression of lux-genes.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S.E., Boyandin A.N., Lankin Yu.P., Kotov D.A., Kargatova T.V., Rodicheva E.K., Popova L.Yu.
Заглавие : Database on natural and transgenic luminous microorganisms: "BIOLUMBASE"
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 2. - С. 278-286. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--database--natural and transgenic luminous microorganisms--photoprotein--article--bacterial gene--bacterium--biotechnology--chemistry--ecology--factual database--genetics--luminescence--transgene--bacteria--biotechnology--databases, factual--ecology--genes, bacterial--luminescence--luminescent proteins--transgenes--bacteria (microorganisms)
Аннотация: The database "BiolumBase" is designed for the selection and systematization of available world information on microorganisms containing bioluminescent systems; it includes two sections: "natural" and "transgenic" luminous microorganisms. At present, logic schemes of divisions, classification of the objects, presentation of characteristics, and the inputs of relative information, as well as the necessary program modules including links to the database, are developed. The database is constructed on the basis of published data and our own experimental results; the subsequent linkage of the database to the Internet is envisaged. Users will be able to obtain not only the catalogues of strains but also information concerning the properties and functions of the known species of luminous bacteria, the structure, regulatory mechanisms, and application of bioluminescent systems and genetically engineered constructions with live genes, as well as to find references and to search strains by using any set of attributes. The database will provide information that is of interest for the development of microbial ecology and biotechnology, in particular, for the prediction of biological hazard from the application of transgenic strains.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla R. M., Williamson C. E., Adamovich B. V., Adrian R., Anneville O., Chandra S., Colom-Montero W., Devlin S. P., Dix M. A., Dokulil M. T., Gaiser E. E., Girdner S. F., Hambright K. D., Hamilton D. P., Havens K., Hessen D. O., Higgins S. N., Huttula T. H., Huuskonen H., Isles P. D.F., Joehnk K. D., Jones I. D., Keller W. B., Knoll L. B., Korhonen J., Kraemer B. M., Leavitt P. R., Lepori F., Luger M. S., Maberly S. C., Melack J. M., Melles S. J., Muller-Navarra D. C., Pierson D. C., Pislegina H. V., Plisnier P. -D., Richardson D. C., Rimmer A., Rogora M., Rusak J. A., Sadro S., Salmaso N., Saros J. E., Saulnier-Talbot E., Schindler D. E., Schmid M., Shimaraeva S. V., Silow E. A., Sitoki L. M., Sommaruga R., Straile D., Strock K. E., Thiery W., Timofeyev M. A., Verburg P., Vinebrooke R. D., Weyhenmeyer G. A., Zadereev E.
Заглавие : Deeper waters are changing less consistently than surface waters in a global analysis of 102 lakes
Место публикации : Sci. Rep.: Nature Research, 2020. - Vol. 10, Is. 1. - Ст.20514. - ISSN 20452322 (ISSN), DOI 10.1038/s41598-020-76873-x
Аннотация: Globally, lake surface water temperatures have warmed rapidly relative to air temperatures, but changes in deepwater temperatures and vertical thermal structure are still largely unknown. We have compiled the most comprehensive data set to date of long-term (1970–2009) summertime vertical temperature profiles in lakes across the world to examine trends and drivers of whole-lake vertical thermal structure. We found significant increases in surface water temperatures across lakes at an average rate of + 0.37 °C decade?1, comparable to changes reported previously for other lakes, and similarly consistent trends of increasing water column stability (+ 0.08 kg m?3 decade?1). In contrast, however, deepwater temperature trends showed little change on average (+ 0.06 °C decade?1), but had high variability across lakes, with trends in individual lakes ranging from ? 0.68 °C decade?1 to + 0.65 °C decade?1. The variability in deepwater temperature trends was not explained by trends in either surface water temperatures or thermal stability within lakes, and only 8.4% was explained by lake thermal region or local lake characteristics in a random forest analysis. These findings suggest that external drivers beyond our tested lake characteristics are important in explaining long-term trends in thermal structure, such as local to regional climate patterns or additional external anthropogenic influences. © 2020, The Author(s).
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Yu., Shpagina V.V., Belyavskaya V.A., Pechurkin N.S.
Заглавие : Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid
Место публикации : Mikrobiologiya. - 1998. - Vol. 67, Is. 2. - С. 170-175. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): catabolite repression--environmental factors--escherichia coli--introduction into model ecosystems--lux-operon--recombinant plasmid--regulation of expression--recombinant dna--article--bacterial gene--chemoluminescence--culture medium--escherichia coli--gene expression regulation--genetics--microbiology--molecular cloning--operon--plasmid--chemiluminescent measurements--cloning, molecular--culture media--dna, recombinant--escherichia coli--gene expression regulation, bacterial--genes, bacterial--operon--plasmids--water microbiology
Аннотация: Expression of the lux-genes cloned on the recombinant plasmid pPHL7 (Ap rLux +) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Yu Krylova T., Kargatova T.V., Yu Popova L., Pechurkin N.S.
Заглавие : Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): genetically modified microorganisms--salinity--salt resistance--sodium chloride--adaptation--article--bacillus subtilis--electrolyte balance--escherichia coli--genetic recombination--genetics--physiology--adaptation, biological--bacillus subtilis--escherichia coli--recombination, genetic--sodium chloride--water-electrolyte balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A. Y., Dementyev D. V., Frolova T. S., Trofimova E. A., Iniatkina E. M., Vasilyev S. A., Sinitsyna O. I.
Заглавие : Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings
Место публикации : Dokl. Biochem. Biophys.: NLM (Medline), 2019. - Vol. 489, Is. 1. - С. 362-366. - ISSN 16083091 (ISSN), DOI 10.1134/S1607672919060024
Аннотация: The effect of ?-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (? 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose ?-radiation on Allium cepa seedlings.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky, A. Ya., Dementyev D. V., Frolova T. S., Trofimova E. A., Iniatkina E. M., Vasilyev S. A., Sinitsyna O. I.
Заглавие : Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings
Колич.характеристики :5 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-240001]; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 489, Is. 1. - С. 362-366. - ISSN 1607-6729, DOI 10.1134/S1607672919060024. - ISSN 1608-3091(eISSN)
Примечания : Cited References:14. - Work on assessing DNA damage in onion seedling nuclei was performed using the equipment of the Core Facility for Microscopic Analysis of Biological Objects, Siberian Branch, Russian Academy of Sciences, funded under the research at the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences. This work was supported in part by the Russian Foundation for Basic Research (project no. 18-44-240001).
Предметные рубрики: COMET ASSAY
REPAIR
CELLS
Аннотация: The effect of gamma-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : U - Popova LYu, Pechurkin N.S., Maksimova E.E., Kargatova T.V. U - Krylova TYu, Lobova T.I., Boyandin A.N.
Заглавие : Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 193-197. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial dna--recombinant dna--adaptation--article--ecosystem--escherichia coli--genetics--microbiology--plasmid--risk assessment--adaptation, biological--dna, bacterial--dna, recombinant--ecosystem--escherichia coli--microbiology--plasmids--risk assessment--soil microbiology--water microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubovtseva I. Y., Aksenenko M. B., Nikolaeva E. D., Averchuk A. S., Moshev A. V., Savchenko A. A., Markova S. V., Ruksha T. G.
Заглавие : FOXC1-Mediated Effects of miR-204-5p on Melanoma Cell Proliferation
Место публикации : Mol Biol (Mosk): NLM (Medline), 2021. - Vol. 55, Is. 4. - С. 667-675. - ISSN 00268984 (ISSN), DOI 10.31857/S0026898421030058
Аннотация: MicroRNAs epigenetically regulate physiological and pathological processes. Previously, we found that miR-204-5p is expressed at low levels in melanoma cells, and an increase in its level leads to a change in proliferation, migration, and invasion of these cancer cells. Now, using bioinformatics analysis, it has been shown that the target of miR-204-5p is FOXC1 transcription factor, which is implicated in carcinogenesis. Using the luciferase reporter assay, it was found that miR-204-5p suppresses expression of the FOXC1 gene by binding to its 3' non-coding region. Transfection of small interfering RNA (siRNA) targeting FOXC1 into melanoma cells caused a decrease in miR-204-5p levels, which is consistent with the generally accepted concept of feedback regulation of miRNA expression by target genes. According to the results of the MTT test and fluorescence microscopy, the proliferation level of melanoma cells under the influence of siRNA to FOXC1 decreased 72 h after transfection. Changes in the ratio of cells by cell cycle phase were analyzed using flow cytometry. Regulatory relationships between FOXC1 and miR-204-5p, and an inhibitory effect of FOXC1 knockdown on melanoma cell proliferation were revealed. Based on the results, it can be assumed that miR-204-5p regulates proliferation of melanoma cells by affecting FOXC1 expression.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A. Y., Sinitsyna O. I., Frolova T. S., Vasyunina E. A., Dementyev D. V.
Заглавие : Genotoxicity assessment of low-level doses of gamma radiation with the SOS chromotest and the Ames test
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2016. - Vol. 469, Is. 1. - С. 309-312. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672916040190
Аннотация: This is the first study to present data on the genotoxicity of low γ-irradiation doses for E. coli and S. typhimurium cells obtained using the SOS chromotest and the Ames test. The most pronounced effect was recorded in the first 24 h of γ-irradiation. After 72 h in the Ames test and after 96 h in the SOS chromotest, a significant effect of γ-irradiation on bacterial cells was detected. The absence of genotoxicity at the later stages can be explained by the adaptation of bacterial cells to the conditions of exposure. The findings allow the bacterial test system to be used for studying the effects of low doses at the early stages of exposure to radiation. © 2016, Pleiades Publishing, Ltd.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla R. M., Mette E. M., Williamson C. E., Adamovich B. V., Adrian R., Anneville O., Balseiro E., Ban S., Chandra S., Colom-Montero W., Devlin S. P., Dix M. A., Dokulil M. T., Feldsine N. A., Feuchtmayr H., Fogarty N. K., Gaiser E. E., Girdner S. F., Gonzalez M. J., Hambright K. D., Hamilton D. P., Havens K., Hessen D. O., Hetzenauer H., Higgins S. N., Huttula T. H., Huuskonen H., Isles P. D.F., Joehnk K. D., Keller W. B., Klug J., Knoll L. B., Korhonen J., Korovchinsky N. M., Koster O., Kraemer B. M., Leavitt P. R., Leoni B., Lepori F., Lepskaya E. V., Lottig N. R., Luger M. S., Maberly S. C., MacIntyre S., McBride C., McIntyre P., Melles S. J., Modenutti B., Muller-Navarra D. C., Pacholski L., Paterson A. M., Pierson D. C., Pislegina H. V., Plisnier P. -D., Richardson D. C., Rimmer A., Rogora M., Rogozin D. Y., Rusak J. A., Rusanovskaya O. O., Sadro S., Salmaso N., Saros J. E., Sarvala J., Saulnier-Talbot E., Schindler D. E., Shimaraeva S. V., Silow E. A., Sitoki L. M., Sommaruga R., Straile D., Strock K. E., Swain H., Tallant J. M., Thiery W., Timofeyev M. A., Tolomeev A. P., Tominaga K., Vanni M. J., Verburg P., Vinebrooke R. D., Wanzenbock J., Weathers K., Weyhenmeyer G. A., Zadereev E. S., Zhukova T. V.
Заглавие : Global data set of long-term summertime vertical temperature profiles in 153 lakes
Место публикации : Sci. Data: Nature Research, 2021. - Vol. 8, Is. 1. - Ст.200. - ISSN 20524463 (ISSN), DOI 10.1038/s41597-021-00983-y
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change. © 2021, The Author(s).
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Raibekas A.A.
Заглавие : Green flavoprotein from P. leiognathi: purification, characterization and identification as the product of the lux G(N) gene
Место публикации : Journal of bioluminescence and chemiluminescence. - 1991. - Vol. 6, Is. 3. - P169-176. - ISSN 08843996 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial protein--flavoprotein--amino acid sequence--article--bacterial gene--chemistry--genetics--isolation and purification--luminescence--molecular genetics--molecular weight--photobacterium--amino acid sequence--bacterial proteins--flavoproteins--genes, bacterial--luminescence--molecular sequence data--molecular weight--photobacterium--support, u.s. gov't, p.h.s.
Аннотация: A green flavoprotein (GFP) was isolated and purified to homogeneity from Photobacterium leiognathi, strain 208. GFP is a homodimer of molecular weight 54,000 and contains two molecules of an unusual flavin per molecule of protein. Various biochemical characteristics including isoelectric point, trypsin and chymotrypsin degradation, SDS and temperature influence on subunit dissociation and the dissociation of the flavin chromophore, were investigated. The sequence of 23 N-terminal amino acids was determined and found to be concurrent with the N-terminal amino acid sequence encoded by the lux G(N) gene of P. leiognathi. This fact suggests that GFP is a structural component of the Photobacterium luminescence system.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wawrzak Z..., Sandalova T..., Steffens J.J., Basarab G.S., Lundqvist T..., Lindqvist Y..., Jordan D.B.
Заглавие : High-resolution structures of scytalone dehydratase-inhibitor complexes crystallized at physiological pH
Колич.характеристики :15 с
Место публикации : Proteins: WILEY-LISS, 1999. - Vol. 35, Is. 4. - P425-439. - ISSN 0887-3585, DOI 10.1002/(SICI)1097-0134(19990601)35:4425::AID-PROT63.0.CO;2-1
Примечания : Cited References: 33
Предметные рубрики: MAGNAPORTHE-GRISEA
HEMAGGLUTININ
GLYCOPROTEIN
REFINEMENT
MELANIN
DISEASE
SITE
Ключевые слова (''Своб.индексиров.''): structure-based design--enzyme inhibitors--x-ray crystallography--fungicides--melanin biosynthesis
Аннотация: Scytalone dehydratase is a molecular target of inhibitor design efforts aimed at preventing the fungal disease caused by Magnaporthe grisea. A method for cocrystallization of enzyme with inhibitors at neutral pH has produced several crystal structures of enzyme-inhibitor complexes at resolutions ranging from 1.5 to 2.2 Angstrom Four high resolution structures of different enzyme-inhibitor complexes are described. In contrast to the original X-ray structure of the enzyme, the four new structures have well-defined electron density for the loop region comprising residues 115-119 and a different conformation between residues 154 and 160. The structure of the enzyme complex with an aminoquinazoline inhibitor showed that the inhibitor is in a position to form a hydrogen bond with the amide of the Asn131 side chain and with two water molecules in a fashion similar to the salicylamide inhibitor in the original structure, thus confirming design principles. The aminoquinazoline structure also allows for a more confident assignment of donors and accepters in the hydrogen bonding network, The structures of the enzyme complexes with two dichlorocyclopropane carboxamide inhibitors showed the two chlorine atoms nearly in plane with the amide side chain of Asn131. The positions of Phe53 and Phe158 are significantly altered in the new structures in comparison to the two structures obtained from crystals grown at acidic pH, The multiple structures help define the mobility of active site amino acids critical for catalysis and inhibitor binding. Proteins 1999;35:425-439. (C) 1999 Wiley-Liss, Inc.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : Information capacity of symbol sequences
Колич.характеристики :13 с
Место публикации : Open Syst. Inf. Dyn.: KLUWER ACADEMIC PUBL, 2002. - Vol. 9, Is. 1. - P37-49. - ISSN 1230-1612, DOI 10.1023/A:1014230928565
Примечания : Cited References: 23
Предметные рубрики: REDUNDANCY
INTRONS
Аннотация: The information capacity of sequences is considered through the calculation of specific entropy of their frequency dictionary. The specific entropy was calculated against the reconstructed dictionary which bears the most probable continuations of shorter strings. The measure developed allows to distinguish the sequences both from the random ones, and those with high level of (rather simple) order. Some applications of the developed methodology to genetics, bioinformatics, and linguistics axe discussed.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.I., Maksimova E.E., Kargatova T.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems
Место публикации : Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk. - 1998. - Is. 6. - С. 670-677. - ISSN 10263470 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bacterial gene--chemoluminescence--escherichia coli--gene expression--genetic recombination--genetics--laboratory--microbiology--chemiluminescent measurements--escherichia coli--gene expression--genes, bacterial--laboratories--recombination, genetic--water microbiology
Аннотация: We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Frolova T., Dementyev D., Sinitsyna O.
Заглавие : Low doses of gamma-radiation induce SOS response and increase mutation frequency in Escherichia coli and Salmonella typhimurium cells
Место публикации : Ecotoxicol. Environ. Saf.: Academic Press, 2016. - Vol. 134. - С. 233-238. - ISSN 01476513 (ISSN) , DOI 10.1016/j.ecoenv.2016.09.009
Ключевые слова (''Своб.индексиров.''): absorbed dose--ames test--dose rate--mutation rate--sos chromotest--ames test--cell death--dna repair--escherichia coli--experimental model--gamma radiation--limit of detection--long term exposure--mutation rate--nonhuman--sos chromotest--bacteria (microorganisms)--escherichia coli--salmonella typhimurium
Аннотация: This study addresses use of two bacterial test systems (the Ames test and the SOS chromotest) to estimate the effects of low doses of γ-radiation. The most substantial increases in induction of SOS response and mutation frequencies were observed in the first 24 h of exposure to γ-radiation as compared to the cells in the exposure-free control. Gamma-radiation also impaired growth and survival of S. typhimurium cells in the first 24 h. The effects were attenuated at lower exposure doses and at longer exposure times. In the experiments conducted in this study, at 96 h of exposure, the values of some of the γ-radiation effects were lower than the MID (minimum inducing dose) detection limits and, thus, were neglected. Long-term exposure to γ-radiation could also result in combined effects of γ-radiation and the death of cells in the culture. © 2016 Elsevier Inc.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Kalacheva G.S., Rodicheva E.K., Volova T.G.
Заглавие : Luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters
Место публикации : Doklady Biochemistry and Biophysics. - 2007. - Vol. 416, Is. 1-6. - С. 248-251. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672907050067
Ключевые слова (''Своб.индексиров.''): photoprotein--polyester--polyhydroxyalkanoic acid--article--bacterium--chemistry--chemoluminescence--classification--genetic procedures--genetics--metabolism--photobacterium--phylogeny--time--bacteria--biosensing techniques--chemiluminescent measurements--luminescent proteins--photobacterium--phylogeny--polyesters--polyhydroxyalkanoates--time factors--bacteria (microorganisms)
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