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Bacterial Cellulose (BC) and BC Composites: Production and Properties / TGG Volova, SVV Prudnikova, EGG Kiselev [et al.] // Nanomaterials. - 2022. - Vol. 12, Is. 2. - Ст. 192, DOI 10.3390/nano12020192. - Cited References:113. - This research was financially supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006. . - ISSN 2079-4991

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
SILVER NANOPARTICLES
GLUCONACETOBACTER-HANSENII
MICROBIAL CELLULOSE
Кл.слова (ненормированные):
bacterial cellulose -- composites -- production -- properties
Аннотация: The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m(2) and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.

WOS
Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys SB, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, LV Kirensky Inst Phys SB, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Sch Petr & Gas Engn, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Volova, Tatiana G. G.; Prudnikova, Svetlana V. V.; Kiselev, Evgeniy G. G.; Nemtsev, Ivan V. V.; Vasiliev, Alexander D. D.; Kuzmin, Andrey P. P.; Shishatskaya, Ekaterina I. I.; Kiselev, Evgeniy; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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Modeling the radial stem growth of the pine (Pinus sylvestris l.) forests using the satellite-derived ndvi and lst (modis/aqua) data / Y. Ivanova, A. Kovalev, V. Soukhovolsky // Atmosphere. - 2021. - Vol. 12, Is. 1. - Ст. 12. - P1-15, DOI 10.3390/atmos12010012 . - ISSN 2073-4433
Кл.слова (ненормированные):
Boreal forests -- Field measurements -- Modeling studies of forest -- Remote sensing data analysis -- Tree and forest functioning -- Tree ring width -- Land surface temperature -- Radiometers -- Satellites -- Time series analysis -- Developed model -- Growing season -- Measurements of -- Normalized difference vegetation index -- Parabolic approximation -- Pinus sylvestris -- Principal Components -- Tree-ring width -- Forestry -- Aqua (satellite) -- boreal forest -- coniferous tree -- growth rate -- land surface -- modeling -- MODIS -- NDVI -- phytomass -- principal component analysis -- remote sensing -- satellite data -- surface temperature -- tree ring -- Pinus sylvestris
Аннотация: The paper considers a new approach to modeling the relationship between the increase in woody phytomass in the pine forest and satellite-derived Normalized Difference Vegetation Index (NDVI) and Land Surface Temperature (LST) (MODIS/AQUA) data. The developed model combines the phenological and forest growth processes. For the analysis, NDVI and LST (MODIS) satellite data were used together with the measurements of tree-ring widths (TRW). NDVI data contain features of each growing season. The models include parameters of parabolic approximation of NDVI and LST time series transformed using principal component analysis. The study shows that the current rate of TRW is determined by the total values of principal components of the satellite indices over the season and the rate of tree increment in the preceding year. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation
Sukachev Institute of Forest SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Ivanova, Y.; Kovalev, A.; Soukhovolsky, V.

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Synthesis and characterization of multicomponent PHAs / E. G. Kiselev, A. D. Vasiliev, T. G. Volova // J. Sib. Fed. Univ. - Biol. - 2021. - Vol. 14, Is. 1. - С. 97-113, DOI 10.17516/1997-1389-0325. - Cited By :1 . - ISSN 1997-1389
Кл.слова (ненормированные):
Biosynthesis -- Copolymers -- Physicochemical properties -- Polyhydroxyalkanoates (PHAs) -- Precursor substrates
Аннотация: Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve polyhydroxyalkanoates (PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids - salts of valeric and hexanoic acids, propionate, and ?-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °C. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Vasiliev, A. D.; Volova, T. G.

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Influence of NaCl on Productivity and Fluorescence Parameters of Nasturtium officinale R. Br. and Its Relevance to Artificial Closed Ecosystems / A. M. Pavlova, N. A. Gaevskii, O. V. Anishchenko [et al.] // Russ. J. Plant Physiol. - 2021. - Vol. 68, Is. 6. - P1173-1185, DOI 10.1134/S1021443721050137. - Cited References:27. - This work was supported by the fundamental research program of the Russian Academy of Sciences for 2013-2020, project no. 56.1.4 Sustainability of Higher Plant Cenoses Grown on Nutrient Media with Mineralized Organic Waste in Closed Human-Inhabited Ecological Systems. . - ISSN 1021-4437. - ISSN 1608-3407

РУБ Plant Sciences
Рубрики:
CHLOROPHYLL FLUORESCENCE
   SALT STRESS
   TOLERANCE
   PHOTOSYNTHESIS
Кл.слова (ненормированные):
Nasturtium officinale -- glycophyte -- salt tolerance -- photosynthetic -- apparatus -- closed ecosystems
Аннотация: Productivity values, sodium accumulation in aboveground biomass, and photosynthetic indices of watercress (Nasturtium officinale) leaves were investigated under conditions resembling artificial closed ecological systems (CES). The seedlings were grown on nutrient media with various NaCl concentrations (0.7, 1.4, and 1.8 g/L) for 7, 14, and 19 days after transferring them to saline solutions. The productivity of plants on the seventh day of their growth on saline media did not differ from that of control plants. The decrease in plant productivity was noted in all the treatments starting from the 14th day after transferring the plants to saline solutions. When NaCl concentration in the nutrient solution was raised from 0.7 to 1.8 g/L, a significant increase in relative Na+ content in plant tissues was observed, regardless of the duration of NaCl treatment. A substantial decrease in chlorophyll (a + b) to carotenoid content ratio was noted on the seventh and 14th days in plants grown at 1.8 g/L NaCl. In plants treated for 7 days with 0.7 and 1.4 g/L NaCl, the content of chlorophylls a and b and carotenoids was found to increase, which indicates the tolerance of N. officinale to CES conditions. The relative content of chlorophylls a and b in the light-harvesting chlorophyll (a + b) complex was independent of the extent of salinity. The maximum quantum yield of photosystem II reaction in N. officinale plants had typically high values (Y(II)(max) of 0.755 +/- 0.007). Using the Imaging Maxi version of the pulse amplitude-modulated (PAM) fluorometer, it was found that light curves for the effective quantum yield of photochemical and nonphotochemical fluorescence quenching (Y(II) and Y(NPQ), respectively) differed appreciably between the salt-treated and untreated plants in the case of long-term cultivation (19 days) at 0.7 and 1.4 g/L NaCl. The treatment with 1.8 g/L NaCl for the period from 14 to 19 days had no effect on light curves of Y(II) and Y(NPQ). It is argued that N. officinale can be used as a source of NaCl for humans under CES conditions.

WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.

Доп.точки доступа:
Pavlova, A. M.; Gaevskii, N. A.; Anishchenko, O. V.; Tikhomirova, N. A.; Tikhomirov, A. A.; fundamental research program of the Russian Academy of SciencesRussian Academy of Sciences [56.1.4]

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Seasonal formation of annual rings on the scales of Baikal grayling inhabiting the middle reaches of the Yenisei River under altered temperature regime / I. V. Zuev, P. Y. Andrushchenko, T. A. Zotina // Environ. Biol. Fishes. - 2021, DOI 10.1007/s10641-021-01155-y . - Article in press. - ISSN 0378-1909
Кл.слова (ненормированные):
No-growth time -- Number of circuli -- Seasonal migration -- Thymallus arcticus -- Thymallus baicalensis
Аннотация: The seasonal formation of annual rings on the scales of Baikal grayling from the middle reaches of the Yenisei River has been studied to find out the reasons for the high growth rate of the grayling under altered temperature regime downstream of the dam of the Krasnoyarsk Hydroelectric Plant. The number of circuli outside the last identified annuli and in the second, third, and fourth completed annuli was estimated on 569 fish caught during the annual cycle. The von Bertalanffy growth function was used to describe the increment in the number of circuli over a year. The calculation showed that a new annual ring was produced in July. In November, there was no statistically significant difference between the circulus number in scale increment and the circulus number in the corresponding completed annuli of older fish. Thus, despite the increased duration of the period with optimal water temperatures downstream of the dam of the Krasnoyarsk Hydroelectric Plant, fish growth was observed in a limited period of the year, from July to November. The probable reason for the high growth rate of grayling in the study area is that the fish do not have to waste energy on seasonal migration to the tributaries. Taking into account that grayling biomass production in the middle Yenisei occurs from July to November, we can recommend shifting the dates of commercial fishing for grayling to the end of this period—November–December. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.

Scopus
Держатели документа:
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny av, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Zuev, I. V.; Andrushchenko, P. Y.; Zotina, T. A.

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Structural Features of Scales of Baikal Grayling Thymallus baicalensis under Conditions of an Altered Hydrological Regime / I. V. Zuev, P. Y. Andrushchenko, S. M. Chuprov, T. A. Zotina // Inland Water Biol. - 2021. - Vol. 14, Is. 1. - P60-66, DOI 10.1134/S1995082920060176 . - ISSN 1995-0829
Кл.слова (ненормированные):
growth rate -- migration -- number of circuli -- water temperature -- Thymallus -- Thymallus thymallus
Аннотация: Abstract: The number of circuli between annual scale rings of grayling Thymallus baicalensis Dybowski, 1874 sampled in the Yenisei River in the downstream section of the Krasnoyarsk Hydroelectric Power Station, which does not freeze in winter, have been investigated and compared with populations of grayling from large tributaries of the Yenisei River (Amyl, Kan, Nizhnyaya Tunguska, and Bolshaya Kheta). It has been shown that graylings inhabiting the Yenisei River have a significantly higher (1.5–2.0 times, p < 0.01) number of circuli in the second, third, and fourth annual rings of the scales than in the populations from the tributaries, which corresponds to a higher growth rate of the grayling population in the Yenisei River. An assumption is made about the transition of graylings inhabiting the Yenisei River channel in the lower reaches of the hydroelectric station to a sedentary lifestyle. © 2021, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zuev, I. V.; Andrushchenko, P. Y.; Chuprov, S. M.; Zotina, T. A.

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Modeling the Radial Stem Growth of the Pine (Pinus sylvestris L.) Forests Using the Satellite-Derived NDVI and LST (MODIS/AQUA) Data / Y. Ivanova, A. Kovalev, V. Soukhovolsky // Atmosphere. - 2021. - Vol. 12, Is. 1. - Ст. 12, DOI 10.3390/atmos12010012. - Cited References:51. - This research was funded by RFBR according to the research project number 18-04-00119-a. . - ISSN 2073-4433

РУБ Environmental Sciences + Meteorology & Atmospheric Sciences

Кл.слова (ненормированные):
boreal forests -- tree and forest functioning -- tree ring width -- field -- measurements -- remote sensing data analysis -- modeling studies of forest
Аннотация: The paper considers a new approach to modeling the relationship between the increase in woody phytomass in the pine forest and satellite-derived Normalized Difference Vegetation Index (NDVI) and Land Surface Temperature (LST) (MODIS/AQUA) data. The developed model combines the phenological and forest growth processes. For the analysis, NDVI and LST (MODIS) satellite data were used together with the measurements of tree-ring widths (TRW). NDVI data contain features of each growing season. The models include parameters of parabolic approximation of NDVI and LST time series transformed using principal component analysis. The study shows that the current rate of TRW is determined by the total values of principal components of the satellite indices over the season and the rate of tree increment in the preceding year.

WOS
Держатели документа:
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Krasnoyarsk 660036, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ivanova, Yulia; Kovalev, Anton; Soukhovolsky, Vlad; RFBRRussian Foundation for Basic Research (RFBR) [18-04-00119-a]

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Biodegradable polymers - Perspectives and applications in agriculture / E. G. Kiselev, N. O. Zhila, T. G. Volova // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст. 012036, DOI 10.1088/1755-1315/689/1/012036
Кл.слова (ненормированные):
Biodegradable polymers -- Ecosystems -- Fungi -- Glycerol -- Monounsaturated fatty acids -- Oilseeds -- Pesticides -- Substrates -- Sunflower oil -- Fenoxaprop-p-ethyl -- Natural materials -- Pesticide formulations -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Productive process -- Strategy of constructions -- Various substrates -- Palm oil
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Zhila, N. O.; Volova, T. G.

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РУБ Marine & Freshwater Biology

Кл.слова (ненормированные):
number of circuli -- water temperature -- growth rate -- migration
Аннотация: The number of circuli between annual scale rings of grayling Thymallus baicalensis Dybowski, 1874 sampled in the Yenisei River in the downstream section of the Krasnoyarsk Hydroelectric Power Station, which does not freeze in winter, have been investigated and compared with populations of grayling from large tributaries of the Yenisei River (Amyl, Kan, Nizhnyaya Tunguska, and Bolshaya Kheta). It has been shown that graylings inhabiting the Yenisei River have a significantly higher (1.5-2.0 times, p < 0.01) number of circuli in the second, third, and fourth annual rings of the scales than in the populations from the tributaries, which corresponds to a higher growth rate of the grayling population in the Yenisei River. An assumption is made about the transition of graylings inhabiting the Yenisei River channel in the lower reaches of the hydroelectric station to a sedentary lifestyle.

WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.

Доп.точки доступа:
Zuev, I., V; Andrushchenko, P. Yu; Chuprov, S. M.; Zotina, T. A.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-240003]; Krasnoyarsk Regional Foundation [18-44-240003]; Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academy of SciencesRussian Academy of Sciences

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10.

Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources / N. O. Zhila, K. Y. Sapozhnikova, E. G. Kiselev [et al.] // Polymers. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142. - Cited References:78. - This work was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties). . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
RALSTONIA-EUTROPHA
   POLY(3-HYDROXYBUTYRATE) PRODUCTION
   PLANT OIL
   ACID
Кл.слова (ненормированные):
Cupriavidus necator IBP -- SFU-1 -- cell growth and PHA synthesis -- various -- carbon sources -- PHA composition and properties -- polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7-8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 +/- 15 kDa) and weight-average (850 +/- 25 kDa) molecular weights and the lowest polydispersity (2.9 +/- 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.

WOS
Держатели документа:
Siberian Fed Univ, Basic Dept Biotechnol, Sch Fundamental Biol & Biotechnol, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, LV Kirensky Inst Phys, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zhila, Natalia O.; Sapozhnikova, Kristina Yu; Kiselev, Evgeniy G.; Vasiliev, Alexander D.; Nemtsev, Ivan, V; Shishatskaya, Ekaterina, I; Volova, Tatiana G.; Russian FederationRussian Federation [075-15-2021-626, 220]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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11.

Phytoplankton and Phytoperiphyton Characteristics of Lake Pyasino and Its Tributaries after an Accidental Fuel Spill in 2020 / E. S. Kravchuk, A. V. Kotovshchikov, E. A. Ivanova // Contemp. Probl. Ecol. - 2021. - Vol. 14, Is. 4. - P368-379, DOI 10.1134/S1995425521040065. - Cited References:30. - The study was supported by economic agreement no. 223-EP-2020/07 with the Siberian Branch of the Russian Academy of Sciences. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
OIL-SPILLS
Кл.слова (ненормированные):
fuel pollution -- freshwater ecosystems -- phytoplankton -- phytoperiphyton -- photosynthetic pigments -- Lake Pyasino -- Ambarnaya River
Аннотация: An assessment of the species composition, abundance, biomass, and pigment characteristics of phytoperiphyton and phytoplankton of Lake Pyasino, its tributaries (Bezymyannyi Stream, Daldykan, Ambarnaya, and Norilskaya rivers), and the head of the Pyasina River has been carried out after a manmade accident (a diesel-fuel spill near the city of Norilsk in May 2020). A significant decline in the biomass and changes in the species composition of phytoperiphyton after the water was contaminated by fuel is revealed only near the spillage site (in the Daldykan River and the Ambarnaya River downstream the mouth of the Daldykan). Downstream, in the Ambarnaya mouth zone, as a response to the release of a large amount of nutrients during the decomposition of fuel products and dead organisms, as well as the mechanical cleaning of the bank line, there has been a massive growth of diatom and green algae (Tabularia tabulata, Spirogyra sp.) in the water column and the appearance of indicators of organic pollution (Euglenophyceae and Cryptophyceae). No significant changes in the plankton of Lake Pyasino and the Pyasina River in comparison with the data obtained in the second half of the 20th century (i.e., long before the accident) are found. Species composition and quantitative features, as well as amount and ratio of pigments, characterized the phytoplankton and phytoperiphyton of Lake Pyasino and the Pyasina River as a normally functioning freshwater community of oligotrophic waters. All this indicates the absence of a negative impact of the accidental fuel spill on the ecosystem of Lake Pyasino and the Pyasina River.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys,Fed Res Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Inst Water & Environm Problems, Barnaul 656038, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kravchuk, E. S.; Kotovshchikov, A. V.; Ivanova, E. A.; Siberian Branch of the Russian Academy of SciencesRussian Academy of Sciences [223-EP-2020/07]

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12.

Fatty acid content and composition in tissues of Baikal grayling (Thymallus baicalensis), with a special focus on DHA synthesis / O. N. Makhutova, K. N. Stoyanov // Aquac. Int. - 2021, DOI 10.1007/s10499-021-00755-w. - Cited References:75. - The research was funded by a grant from the Russian Foundation for Basic Research (RFBR) N 20-04-00594, by the state assignment within the framework of the Basic Research Program of the Russian Federation (topic no. 51.1.1) and the state assignment of the Ministry of Science and Higher Education of the Russian Federation to Siberian Federal University in 2020 (Project no. FSRZ-2020-0006 "Biologically active substances in environmental, biotechnological and medical systems"). . - Article in press. - ISSN 0967-6120. - ISSN 1573-143X

РУБ Fisheries
Рубрики:
COD GADUS-MORHUA
   DOCOSAHEXAENOIC ACID
   SEASONAL DYNAMICS
   FISH
Кл.слова (ненормированные):
Docosahexaenoic fatty acid -- Sprecher pathway -- Fish diet -- Furan fatty -- acids -- Aquaculture
Аннотация: Long-chain polyunsaturated fatty acids of n-3 family (n-3 LC PUFAs) are physiologically essential compounds required for normal growth and development of animals, including humans. The ability of fish species to synthesize n-3 LC PUFAs varies significantly across different trophic levels. We have studied fatty acid (FA) content (mg/g of wet weight) and level (% of total FAs) in the brain, liver, heart, intestine, female and male gonads, muscle, and adipose tissues of commercially important wild freshwater Baikal grayling. Additionally, FA content and level of Baikal grayling juveniles have been studied. In all tissues of Baikal grayling, some LC PUFAs, namely, 24:5n-3 and 24:6n-3 (C24 PUFAs), have been found. These FAs are the intermediate products in the synthesis of docosahexaenoic acid (DHA, 22:6n-3) by the Sprecher pathway. The levels of C24 PUFAs in tissues differed significantly: the highest levels of C24 PUFAs were found in adipose tissue and the lowest values in the gonads of females, liver, brain, and head of juveniles. According to the dynamics of DHA and C24 PUFAs, the maximum rate of DHA synthesis is achieved in brain of Baikal grayling, while the lowest rate of DHA synthesis probably occurs in adipose tissue. Although all studied tissues had differences in the number of FAs and their levels, 16:0, 18:1n-9, 16:1n-7, 20:5n-3, and DHA dominated. Male gonads contained an extremely high level of furan FAs - presumably beneficial substances for human health. Additionally, the nutritional value of the tissues of Baikal grayling as a source of n-3 LC PUFAs for humans has been estimated.

WOS
Держатели документа:
Russian Acad Sci Akademgorodok, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Makhutova, Olesia N.; Stoyanov, Kirill N.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [20-04-00594]; Russian FederationRussian Federation [51.1.1]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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13.

Fatty acid content and composition in tissues of Baikal grayling (Thymallus baicalensis), with a special focus on DHA synthesis / O. N. Makhutova, K. N. Stoyanov // Aquac. Int. - 2021, DOI 10.1007/s10499-021-00755-w . - Article in press. - ISSN 0967-6120
Кл.слова (ненормированные):
Aquaculture -- Docosahexaenoic fatty acid -- Fish diet -- Furan fatty acids -- Sprecher pathway
Аннотация: Long-chain polyunsaturated fatty acids of n-3 family (n-3 LC PUFAs) are physiologically essential compounds required for normal growth and development of animals, including humans. The ability of fish species to synthesize n-3 LC PUFAs varies significantly across different trophic levels. We have studied fatty acid (FA) content (mg/g of wet weight) and level (% of total FAs) in the brain, liver, heart, intestine, female and male gonads, muscle, and adipose tissues of commercially important wild freshwater Baikal grayling. Additionally, FA content and level of Baikal grayling juveniles have been studied. In all tissues of Baikal grayling, some LC PUFAs, namely, 24:5n-3 and 24:6n-3 (C24 PUFAs), have been found. These FAs are the intermediate products in the synthesis of docosahexaenoic acid (DHA, 22:6n-3) by the Sprecher pathway. The levels of C24 PUFAs in tissues differed significantly: the highest levels of C24 PUFAs were found in adipose tissue and the lowest values in the gonads of females, liver, brain, and head of juveniles. According to the dynamics of DHA and C24 PUFAs, the maximum rate of DHA synthesis is achieved in brain of Baikal grayling, while the lowest rate of DHA synthesis probably occurs in adipose tissue. Although all studied tissues had differences in the number of FAs and their levels, 16:0, 18:1n-9, 16:1n-7, 20:5n-3, and DHA dominated. Male gonads contained an extremely high level of furan FAs — presumably beneficial substances for human health. Additionally, the nutritional value of the tissues of Baikal grayling as a source of n-3 LC PUFAs for humans has been estimated. © 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.

Scopus
Держатели документа:
Institute of Biophysics of Federal Research Center “Krasnoyarsk Science Center” of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Makhutova, O. N.; Stoyanov, K. N.

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14.

РУБ Ecology + Marine & Freshwater Biology
Рубрики:
THYMALLUS-ARCTICUS
   FLOW REGULATION
   SOCKEYE-SALMON
   SOMATIC GROWTH
Кл.слова (ненормированные):
Thymallus baicalensis -- Thymallus arcticus -- Number of circuli -- No-growth -- time -- Seasonal migration
Аннотация: The seasonal formation of annual rings on the scales of Baikal grayling from the middle reaches of the Yenisei River has been studied to find out the reasons for the high growth rate of the grayling under altered temperature regime downstream of the dam of the Krasnoyarsk Hydroelectric Plant. The number of circuli outside the last identified annuli and in the second, third, and fourth completed annuli was estimated on 569 fish caught during the annual cycle. The von Bertalanffy growth function was used to describe the increment in the number of circuli over a year. The calculation showed that a new annual ring was produced in July. In November, there was no statistically significant difference between the circulus number in scale increment and the circulus number in the corresponding completed annuli of older fish. Thus, despite the increased duration of the period with optimal water temperatures downstream of the dam of the Krasnoyarsk Hydroelectric Plant, fish growth was observed in a limited period of the year, from July to November. The probable reason for the high growth rate of grayling in the study area is that the fish do not have to waste energy on seasonal migration to the tributaries. Taking into account that grayling biomass production in the middle Yenisei occurs from July to November, we can recommend shifting the dates of commercial fishing for grayling to the end of this period-November-December.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, 79 Svobodny, Krasnoyarsk 660041, Russia.
Inst Biophys, Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zuev, Ivan V.; Andrushchenko, Pavel Yu.; Zotina, Tatiana A.; Russian Foundation for Basic Research, Government of the Krasnoyarsk Territory; Krasnoyarsk Regional Scientific Foundation [20-44-240009]

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15.

Properties of degradable polyhydroxyalkanoates (Phas) synthesized by a new strain, cupriavidus necator ibp/sfu-1, from various carbon sources / N. O. Zhila, K. Yu. Sapozhnikova, E. G. Kiselev [et al.] // Polym. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142 . - ISSN 2073-4360
Кл.слова (ненормированные):
Cell growth and PHA synthesis -- Cupriavidus necator IBP/SFU-1 -- PHA composition and properties -- Polymer films -- Various carbon sources -- Biodegradable polymers -- Carbon -- Carbon films -- Cell proliferation -- Crystallinity -- Fructose -- Glucose -- Long Term Evolution (LTE) -- Oleic acid -- Organic carbon -- Palm oil -- Polydispersity -- Semiconducting films -- Autotrophics -- Carbon source -- Cell growth and PHA synthesis -- Cupriavidu necator IBP/SFU-1 -- PHA composition and property -- Plant oil -- Polyhydroxyalkanoates -- Property -- Synthesised -- Various carbon source -- Polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, L.V. Kirensky Institute of Physics SB RAS, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Zhila, N. O.; Sapozhnikova, K. Yu.; Kiselev, E. G.; Vasiliev, A. D.; Nemtsev, I. V.; Shishatskaya, E. I.; Volova, T. G.

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16.

Single-Crystal Diamond Needle Fabrication Using Hot-Filament Chemical Vapor Deposition / R. Ismagilov, S. Malykhin, A. Puzyr [et al.] // Materials. - 2021. - Vol. 14, Is. 9. - Ст. 2320, DOI 10.3390/ma14092320. - Cited References:32. - This work was supported by the Russian Science Foundation (project no. 19-79-00203) and by the Russian Foundation for Basic Research (grant no. 18-29-19071, in part for PL and Raman inspection). . - ISSN 1996-1944

РУБ Chemistry, Physical + Materials Science, Multidisciplinary + Metallurgy &

Кл.слова (ненормированные):
thin films -- diamond needles -- chemical vapor deposition -- hot-filament -- CVD -- large-scale synthesis
Аннотация: Single-crystal diamonds in the form of micrometer-scale pyramids were produced using a combination of hot-filament (HF) chemical vapor deposition (CVD) and thermal oxidation processes. The diamond pyramids were compared here with similar ones that were manufactured using plasma-enhanced (PE) CVD. The similarities revealed in the morphology, Raman, and photoluminescent characteristics of the needles obtained using the hot-filament and plasma-enhanced CVD are discussed in connection with the diamond film growth mechanism. This work demonstrated that the HF CVD method has convincing potential for the fabrication of single-crystal diamond needles in the form of regularly shaped pyramids on a large surface area, even on non-conducting substrates. The experimental results demonstrated the ability for the mass production of the single-crystal needle-like diamonds, which is important for their practical application.

WOS
Держатели документа:
Moscow MV Lomonosov State Univ, Dept Phys, Moscow 119991, Russia.
Univ Eastern Finland, Dept Phys & Math, Joensuu 80101, Finland.
Russian Acad Sci, Lebedev Phys Inst, Div Solid State Phys, Moscow 119991, Russia.
Russian Acad Sci, RAS, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr SB, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ismagilov, Rinat; Malykhin, Sergei; Puzyr, Aleksey; Loginov, Artem; Kleshch, Victor; Obraztsov, Alexander; Russian Science FoundationRussian Science Foundation (RSF) [19-79-00203]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19071]

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17.

Cupriavidus necator B-10646 growth and polyhydroxyalkanoates production on different plant oils / T. Volova, K. Sapozhnikova, N. Zhila // Int. J. Biol. Macromol. - 2020. - Vol. 164. - P121-130, DOI 10.1016/j.ijbiomac.2020.07.095. - Cited References:52. - This studywas financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis fromplant oils), and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (polymer properties). . - ISSN 0141-8130. - ISSN 1879-0003

РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
FATTY-ACID-COMPOSITION
PHA SYNTHASE GENE
PALM KERNEL OIL
Кл.слова (ненормированные):
Cupriavidus necator B-10646 -- Plant oils -- Polyhydroxyalkanoates -- Fatty -- acids -- Emulsifiers
Аннотация: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate was palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9-1.9 mol%) and 3-hydroxyhexanoate (0.5-1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier. (C) 2020 Elsevier B.V. All rights reserved.

WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akad Gorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana; Sapozhnikova, Kristina; Zhila, Natalia; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation; State assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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18.

The effect of deicing salt solutes on Moina macrocopa and Allium cepa in a toxicity test experiment / T. S. Lopatina, Y. V. Aleksandrova, O. V. Anishchenko [и др.] // Vestn. Tomsk. Gos. Univ. Biol. - 2020. - Is. 51. - С. 162-178, DOI 10.17223/19988591/51/9. - Cited References:29. - This work was supported by the joint grant from the Russian Foundation for Basic Research, the Government of Krasnoyarsk Krai, and the Krasnoyarsk Krai Fund for Supporting Scientific and Scientific-Technical Activities (Grant No 19-44-240014). . - ISSN 1998-8591. - ISSN 2311-2077

РУБ Biology + Ecology
Рубрики:
DAPHNIA-MAGNA
   NACL SALINITY
   ROAD SALTS
   WATER
   FRESH
   CHLORIDE
Кл.слова (ненормированные):
salinity -- toxicity test -- Cladocera -- Allium-test -- aquatic ecosystems
Аннотация: Chloride salts are the most commonly used deicing materials for winter maintenance of roads. Numerous studies indicate a significant increase in the salinity of aquatic ecosystems associated with the long-term use of deicing materials in countries located in cold climates. The functioning of ecosystems largely depends on salinity, since salinity is one of the key factors determining the species composition, the structure of food webs and the productivity of aquatic communities. Given the growing threat of salinization of groundwater and surface waters, it is extremely important to study the effect of deicing materials on the biota and functioning of aquatic ecosystems. The aim of this research is to determine the threshold concentrations of solutions of the deicing salt mixture "Bionord" containing sodium and calcium chlorides, at which negative effects on the development of animal and plant test objects are observed. In this study, we used the salt-containing mixture "Bionord" as a model deicer. Similarly, with the most commonly used ice melting chemicals, the "Bionord" salt mixture contains a large amount of sodium and calcium chlorides (about 85% of the total weight). To evaluate the toxicity of the deicer solutions, we used acute and chronic toxicity tests with cladoceran Moina macrocopa (Straus, 1820) (Cladocera: Moinidae) and standard onion-based test with Allium cepa L. (Liliopsida: Amaryllidaceae) (Allium-test). In acute and chronic toxicity tests with Cladocera, the females on the first day of their life (body size 0.5-0.6 mm) were placed individually in jars with aged (not less than for 72 h) tap water with a volume of 20 ml with the addition of a deicer at a certain concentration. A group of animals that was placed in the medium without the deicer was used as a control. In the acute toxicity tests, we used the following concentration of the deicer: 1.3; 2.5; 4.0, 5.0; 6.0; 8.0; 10.0 g/l. The mortality of animals was recorded 24 and 48 hours after the start of the experiment. The concentration of the deicer (LC50) at which 50% of animals was observed to die, compared to the control, was determined in the acute toxicity test. In a chronic toxicity test, animals were tested in the following range of concentrations of the deicer: 0.3; 0.6; 1.3; 2.5; 5.0; 6.0 and 8.0 g/l. The chronic toxicity test was conducted until the death of all test animals. Based on the data obtained in the chronic toxicity test, the specific growth rate of juvenile females, average fecundity, and average life span of M. macrocopa were calculated for each concentration of the deicer. Bulbs of onions of the Stuttgartenrisen variety with a diameter of 1.8 +/- 0.1 cm and a weight of 2.27 +/- 0.17 g were used in the onion test. Bulbs with their bottoms were placed in test tubes containing 20 ml of a solute of the deicer or tap water for 48 hours. Three bulbs were tested for each concentration and for the control. The following concentrations of the deicer were used in the onion test: 1.0; 2.5; 5.0; 7.0; 10.0; 15.0; 20.0; 50.0 g/l. The general toxic and cytotoxic effects were evaluated in the onion test. The average root length and the total root length on each bulb were used as indicators of the total toxicity of the solutions of deicer. To evaluate proliferative activity, we calculated the mitotic index as the fraction of dividing cells in the apical root meristem to the total number of cells. Based on the results of the experiments, we determined median effective mixture concentrations (EC50) at which there is a 50% decrease, compared to the control, in the values of root growth indicators: average root length, sum of root lengths on each bulb and mitotic index. Median lethal concentration (LC50) of the deicing salt determined in the 48-hour acute toxicity test with females of Al. macrocopa was equal to 5.1 g/l. In the chronic test, we showed that the exposure to the solutions of the deicing salt in the range of concentrations from 0.3 to 5.0 g/l does not affect the life span, specific growth rate of juveniles and fecundity of females of M. macrocopa. The median effective concentration (EC50) of the deicing salt determined in the Allium-tests were 6.3, 5.2 and 10.4 g/l for the sum of root lengths, average root length on each bulb and proliferative activity at the tips of roots (mitotic index), respectively (See Table 1 and 2). Complete inhibition of onion root growth was observed at the concentration of the decider equal to 20 g/l, while the death of all test animals in the acute toxicity test occurred at the concentration of the deicer equal to 8,0 g/l (See Fig. 1). Thus, we demonstrated that similar concentrations of the deicer induced 50% inhibition of the growth of onion roots and 50% mortality of cladocerans. These values, in general, corresponded to a critical salinity of 5-8 %o above which qualitative changes occur both in the external and internal condition of aquatic animals. The electrical conductivity of the deicer solutions, which had a negative effect on the selected test species, coincides with the previously obtained values of the electrical conductivity of sodium chloride solutions harmful to cladocerans. We can assume that the main mechanism of the effect of the deicing material that we study is associated with the biological effect of its chlorine and sodium salts Taking this into account, the value of electrical conductivity measured for solutions of deicing salt can be used to assess its negative potential effects. We estimated that in the absence of timely cleaning, regulated by the rules for using the material, the runoff from each square meter of the treated surface can lead to the pollution of 8-13 liters of fresh water. Thus, the basic requirement for the use of deicing salts on roads is the need to comply with the cleaning regime of the treated surfaces. Otherwise, the gradual accumulation of sodium and calcium chlorides in water bodies can cause an increase in salinity which will affect the survival of freshwater aquatic organisms and lead to serious disturbances in the functioning of aquatic ecosystems.

WOS
Держатели документа:
Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Sci Ctr,Lab Ecosyst B, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Sci Ctr,Lab Biolumine, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Sci Ctr,Analyt Lab, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Dept Biophys, 79 Svobodniy Ave, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Lopatina, Tatiana S.; Aleksandrova, Yuliyana, V; Anishchenko, Olesya, V; Gribovskaya, Iliada, V; Oskina, Nataliya A.; Zotina, Tatiana A.; Zadereev, Egor S.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk Krai; Krasnoyarsk Krai Fund for Supporting Scientific and Scientific-Technical Activities [19-44-240014]

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19.

The long-term effects of gamma-radiation on the growth of Allium cepa plants / A. Bolsunovsky, E. Trofimova, D. Dementyev, M. Petrichenkov // Int. J. Radiat. Biol. - 2020, DOI 10.1080/09553002.2021.1844337. - Cited References:28. - The study was partly supported by grant of the Krasnoyarsk Regional Fund of Science - Russian Foundation for Basic Research No. 18-44-240001. . - Article in press. - ISSN 0955-3002. - ISSN 1362-3095

РУБ Biology + Nuclear Science & Technology + Radiology, Nuclear Medicine &
Рубрики:
IONIZING-RADIATION
   PISUM
   ABERRATIONS
   MECHANISMS
   MERISTEM
   SEEDS
Кл.слова (ненормированные):
Allium cepa -- onion seedlings -- gamma-radiation -- low dose -- dose dependency
Аннотация: Purpose The purpose of this study was to assess the long-term effects of gamma-radiation, including low-dose radiation, on growth parameters of onion (Allium cepa) seedling roots 6-10 days after irradiation. Materials and methods Onion seedlings were exposed to a Cs-137 gamma source at doses ranging from 0.1 to 10 grays (Gy). Responses of root and shoot length growth were studied 6 and 10 days after irradiation. Results Our results showed inhibition of the root and shoot length growth 6 days after exposure at all doses, including the low dose - 0.1 Gy. At a later point in time (day 10), root and shoot inhibition was only observed after irradiation at high doses (above 5 Gy), and that suggested the occurrence of cell repair after irradiation at low doses. The results indicated that the length of seedling roots was more sensitive to gamma-irradiation than the shoot length. Conclusion The results of the study suggested that short-term gamma-irradiation of onion seedlings (absorbed doses of 0.1-10 Gy) caused inhibition of plant growth 6 and 10 days after irradiation. The dose dependence of the onion root length was linear. The present study showed for the first time that short-term low-dose gamma-irradiation could induce long-term negative effects on plant growth.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Budker Inst Nucl Phys, Novosibirsk, Russia.

Доп.точки доступа:
Bolsunovsky, Alexander; Trofimova, Elena; Dementyev, Dmitry; Petrichenkov, Michail; Krasnoyarsk Regional Fund of Science - Russian Foundation for Basic Research [18-44-240001]

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20.

Poly-3-hydroxybutyrate/chitosan composite films and nonwoven mats / A. A. Sukhanova, A. E. Murzova, A. N. Boyandin [et al.] // Int. J. Biol. Macromol. - 2020. - Vol. 165. - P2947-2956, DOI 10.1016/j.ijbiomac.2020.10.177. - Cited References:54. - This study was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation to the team of the research laboratory "Intellectual Materials and Structures" within the project "Development of the methods for modifying polymer matrices containing particulate and fibrous fillers of various origins for creating smart multifunctional materials" (subject number FEFE-2020-0015). . - ISSN 0141-8130. - ISSN 1879-0003

РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
BIODEGRADABLE POLY-3-HYDROXYBUTYRATE
BIOMEDICAL APPLICATIONS
BACTERIAL
Кл.слова (ненормированные):
Composites -- Poly-3-hydroxybutyrate -- Chitosan -- Films -- Nonwoven mats -- Physicochemical properties -- Tissue engineering
Аннотация: Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated. DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively. Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm. The values of water contact angle for films (53 degrees) and nonwoven mats (50.6 degrees) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials. On the other hand, P(3HB) showed its reinforcing ability and improved the physical/mechanical properties of chitosan. The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture. (c) 2020 Published by Elsevier B.V.

WOS
Держатели документа:
Reshetnev Siberian State Univ Sci & Technol, 31 Krasnoyarsky Rabochy Av, Krasnoyarsk 660037, Russia.
Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Siberian Branch, Krasnoyarsk Sci Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Sukhanova, A. A.; Murzova, A. E.; Boyandin, A. N.; Kiselev, E. G.; Sukovatyi, A. G.; Kuzmin, A. P.; Shabanov, A., V; Ministry of Education and Science of the Russian FederationMinistry of Education and Science, Russian Federation [FEFE-2020-0015]

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