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Intracellular phosphorus pool of the cyanobacterium spirulina platensis / A. Ya. Bolsunovsk, S. V. Kosinenko // Mikrobiologiya. - 2000. - Vol. 69, Is. 1. - С. 135-137 . - ISSN 0026-3656
Кл.слова (ненормированные):
phosphorus -- article -- biomass -- Cyanobacterium -- growth, development and aging -- metabolism -- Biomass -- Cyanobacteria -- Phosphorus
Аннотация: An intracellular phosphorus pool in a monoculture of the cyanobacterium Spirulina platensis was assessed using radioactive and nonradioactive phosphorus. The derived dependence of specific growth rate on the intracellular content of mineral phosphorus can be presented in the form of the Droop equation. It was found that the stage of replenishment of the intracellular phosphorus pool may affect the phosphorus turnover estimation in aquatic environments from the results of short-term measurements of phosphorus uptake.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsk, A.Ya.; Kosinenko, S.V.

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Chemical fractionation of radionuclides and stable elements in aquatic plants of the yenisei river / A. Bolsunovsky // Environmental Science and Technology. - 2011. - Vol. 45, Is. 17. - P7143-7150, DOI 10.1021/es2008853 . - ISSN 0013-936X
Кл.слова (ненормированные):
Aquatic plants -- Artificial radionuclides -- Cell structure -- Chemical Fractionation -- Distribution patterns -- Nuclear plant -- Plant biomass -- Sequential extraction scheme -- Stable elements -- Submerged plants -- Transuranic elements -- Americium -- Biomass -- Neptunium -- River pollution -- Rivers -- Radioisotopes -- americium -- element -- neptunium -- radioisotope -- bioaccumulation -- chemical binding -- gamma ray spectrometry -- isotopic fractionation -- monocotyledon -- phytomass -- radionuclide -- river pollution -- submerged vegetation -- aquatic species -- article -- Batrachium kauffmanii -- biomass -- cell structure -- controlled study -- extraction -- Fontinalis antipyretica -- fractionation -- gamma spectrometry -- higher plant -- nonhuman -- plant growth -- Potamogeton lucens -- Potamogetonaceae -- river -- Russian Federation -- Aquatic Organisms -- Biomass -- Chemical Fractionation -- China -- Industrial Waste -- Plants -- Radiation Monitoring -- Radioisotopes -- Rivers -- Water Pollutants, Radioactive -- Russian Federation -- Yenisei River -- Batrachium -- Fontinalis antipyretica -- Potamogeton lucens
Аннотация: The Yenisei River is contaminated with artificial radionuclides released by one of the Russian nuclear plants. The aquatic plants growing in the radioactively contaminated parts of the river contain artificial radionuclides. The aim of the study was to investigate accumulation of artificial radionuclides and stable elements by submerged plants of the Yenisei River and estimate the strength of their binding to plant biomass by using a new sequential extraction scheme. The aquatic plants sampled were: Potamogeton lucens, Fontinalis antipyretica, and Batrachium kauffmanii. Gamma-spectrometric analysis of the samples of aquatic plants has revealed more than 20 radionuclides. We also investigated the chemical fractionation of radionuclides and stable elements in the biomass and rated radionuclides and stable elements based on their distribution in biomass. The greatest number of radionuclides strongly bound to biomass cell structures was found for Potamogeton lucens and the smallest for Batrachium kauffmanii. For Fontinalis antipyretica, the number of distribution patterns that were similar for both radioactive isotopes and their stable counterparts was greater than for the other studied species. The transuranic elements 239Np and 241Am were found in the intracellular fraction of the biomass, and this suggested their active accumulation by the plants. В© 2011 American Chemical Society.

Scopus
Держатели документа:
Institute of Biophysics SB, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.

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Intracellular phosphorus pool of the cyanobacterium spirulina platensis / A. Ya. Bolsunovskii, S. V. Kosinenko // Microbiology. - 2000. - Vol. 69, Is. 1. - P116-118 . - ISSN 0026-2617
Аннотация: An intracellular phosphorus pool in a monoculture of the cyanobacterium Spirulina platensis was assessed using radioactive and nonradioactive phosphorus. The derived dependence of specific growth rate on the intracellular content of mineral phosphorus can be presented in the form of the Droop equation. It was found that the stage of replenishment of the intracellular phosphorus pool may affect the phosphorus turnover estimation in aquatic environments from the results of short-term measurements of phosphorus uptake. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Kosinenko, S.V.

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Developing the control criterion for a continuous culture of microorganisms / V. V. Adamovich, D. Yu. Rogozin, A. G. Degermendzhi // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 5-16 . - ISSN 0026-3656
Кл.слова (ненормированные):
Chemostat -- Control criterion -- Control factor -- Microorganism population -- Sensitivity coefficients -- algorithm -- bacterium -- biological model -- biomass -- culture medium -- ecosystem -- growth, development and aging -- methodology -- microbiological examination -- review -- Algorithms -- Bacteria -- Bacteriological Techniques -- Biomass -- Culture Media -- Ecosystem -- Models, Biological
Аннотация: A short survey and critical analysis of previously proposed criteria for growth control of populations of microorganisms in the chemostat are presented. Based on the analysis of a mathematical model of the steady-state of a microbial population in the chemostat, an adequate control criterion is suggested, along with a method to identify the corresponding regulating factors. The new control criterion is expressed as a product of the factor transformation coefficient and the biomass sensitivity coefficient (SC) with respect to the change of the factor at the chemostat inlet (referred to in the sequel as the biomass SC). The control criterion determines the strength of the control exerted by this or that factor. The method of determination of the regulating factors consists in experimental determination of the real SCs for factors and the biomass and in calculating on this basis the corresponding ideal SCs for constant factor transformation coefficients. The ideal SCs are shown to add up to an integer value, a constraint that we call "quantization" relationships. Such relationships are used to test the completeness of the drawn list of control factors. The proposed method was applied to our own and literature data.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Adamovich, V.V.; Rogozin, D.Yu.; Degermendzhi, A.G.

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Global minimal model of perennial dynamics of carbon in biosphere / S. I. Bartsev, A. G. Degermendzhi, D. V. Erokhin // Doklady Akademii Nauk. - 2005. - Vol. 401, Is. 2. - С. 233-237 . - ISSN 0869-5652
Кл.слова (ненормированные):
Atmospheric temperature -- Carbon -- Climatology -- Ecology -- Greenhouse effect -- Thermal effects -- Biosphere -- Carbon perennial dynamics -- Biology
Аннотация: The suggested model is not intended for describing most probable biosphere dynamics but only for estimating the possibility of catastrophic variant of biosphere dynamics development at antropogenic CO2 emission into atmosphere due to different temperature dependences for key biological biosphere component growth. The main result is demonstration of the possibility of irreversible climate changes and biosphere destruction resulting from interaction of biological processes with greenhouse effect.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bartsev, S.I.; Degermendzhi, A.G.; Erokhin, D.V.

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Developing the control criterion for a continuous culture of microorganisms / V. V. Adamovich, D. Yu. Rogozin, A. G. Degermendzhi // Microbiology. - 2005. - Vol. 74, Is. 1. - P1-11, DOI 10.1007/s11021-005-0021-z . - ISSN 0026-2617
Кл.слова (ненормированные):
Chemostat -- Control criterion -- Control factor -- Microorganism population -- Sensitivity coefficients
Аннотация: A short survey and a critical analysis of previously proposed criteria for growth control of populations of microorganisms in the chemostat are presented. Based on the analysis of a mathematical model of the steady state of a microbial population in the chemostat, an adequate control criterion is suggested, along with a method to identify the corresponding control factors. The new control criterion is expressed as a product of the factor transformation coefficient and the sensitivity coefficient (SC) of the biomass with respect to the change in the factor at the chemostat inlet (hereinafter, the biomass SC). The control criterion determines the strength of the control exerted by a factor. The method of determining control factors consists in experimental determination of the real SCs for factors and the biomass and calculation on this basis of the corresponding ideal SCs, assuming constant factor transformation coefficients. The ideal SCs are shown to add up to integer values, a constraint that we call quantization relationships. Such relationships are used to test the completeness of the list of control factors. The proposed method was applied to both our own and literature data. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Adamovich, V.V.; Rogozin, D.Yu.; Degermendzhi, A.G.

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Anoxygenic phototrophic bacteria in meromictic lakes of southern Siberia during the ice period: Spatial distributions and ecological conditions / D. Yu. Rogozin [et al.] // Doklady Biological Sciences. - 2009. - Vol. 424, Is. 1. - P63-67, DOI 10.1134/S0012496609010190 . - ISSN 0012-4966
Кл.слова (ненормированные):
fresh water -- anaerobic growth -- article -- environmental aspects and related phenomena -- history -- ice cover -- microbiology -- phototrophy -- Russian Federation -- season -- Anaerobiosis -- Ecological and Environmental Phenomena -- Fresh Water -- History, Ancient -- Ice Cover -- Phototrophic Processes -- Seasons -- Siberia -- Photobacteria

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk 66041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rogozin, D.Yu.; Zykov, V.V.; Chernetskii, M.Yu.; Degermendzhy, A.G.

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241Am distribution in the biomass of freshwater macrophytes / T. A. Zotina [et al.] // Doklady Biological Sciences. - 2008. - Vol. 421, Is. 1. - P254-256, DOI 10.1134/S0012496608040108 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- carbohydrate -- cellulose -- lipid -- nitrogen -- polysaccharide -- vegetable protein -- article -- biomass -- Bryopsida -- cell membrane -- cell wall -- chemistry -- cytoplasm -- food chain -- growth, development and aging -- Hydrocharitaceae -- metabolism -- Americium -- Biomass -- Bryopsida -- Carbohydrates -- Cell Membrane -- Cell Wall -- Cellulose -- Cytoplasm -- Food Chain -- Hydrocharitaceae -- Lipids -- Nitrogen -- Plant Proteins -- Polysaccharides

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Kalachova, G.S.; Bolsunovsky, A.Ya.; Degermendzhy, A.G.

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The "quantization" of sensitivity coefficients is preserved in microbial populations heterogeneous with respect to growth rate and age / V. M. Nekrasov, A. V. Chernychev, A. G. Degermendzhy // Doklady Biological Sciences. - 2006. - Vol. 406, Is. 1-6. - P91-93, DOI 10.1134/S0012496606010261 . - ISSN 0012-4966
Кл.слова (ненормированные):
algorithm -- article -- bacterium -- biodiversity -- growth, development and aging -- population dynamics -- theoretical model -- time -- Algorithms -- Bacteria -- Biodiversity -- Models, Theoretical -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Institute of Chemical Kinetics and Combustion, Siberian Division, Russian Academy of Sciences, Institutskaya ul. 3, Novosibirsk, 630090, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nekrasov, V.M.; Chernychev, A.V.; Degermendzhy, A.G.

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10.

Self-restoration as fundamental property of CES providing their sustainability / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1641-1648, DOI 10.1016/S0273-1177(03)00111-X . - ISSN 0273-1177
Кл.слова (ненормированные):
Biocatalysts -- Ecosystems -- Genes -- Life support systems (spacecraft) -- Radiation damage -- Cell populations -- Space flight -- space shuttle -- article -- biological model -- biomass -- cell division -- Chlorella -- cytology -- growth, development and aging -- microclimate -- radiation exposure -- radiation response -- ultraviolet radiation -- Biomass -- Cell Division -- Chlorella -- Dose-Response Relationship, Radiation -- Ecological Systems, Closed -- Life Support Systems -- Models, Biological -- Ultraviolet Rays
Аннотация: Sustainability is one of the most important criteria and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.

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11.

Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia) / A. G. Degermendzhy, R. D. Gulati // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P331-340 . - ISSN 1386-2588
Кл.слова (ненормированные):
Carbon budget -- Cyanobacteria -- Heterotrophic bacteria -- Hydrogen sulphide -- Mathematical models of stratification -- Meromictic lakes -- Microbial loop -- Stratification -- Trophic scheme -- algal bloom -- ecosystem modeling -- limiting factor -- nutrient availability -- phytoplankton -- saline lake -- trophic interaction -- Russian Federation -- algae -- Bacteria (microorganisms) -- Cyanobacteria -- Lyngbya -- Lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Gulati, R.D.

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12.

Formation of the vertical heterogeneity in the Lake Shira ecosystem: The biological mechanisms and mathematical model / A. G. Degermendzhy [et al.] // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P271-297, DOI 10.1023/A:1015621508971 . - ISSN 1386-2588
Кл.слова (ненормированные):
Hydrogen sulphide -- Phytoplankton -- Stratification control -- Sulphate-reducing bacteria -- Sulphur cycle -- Vertical model -- biological production -- community structure -- ecosystem modeling -- nutrient cycling -- plankton -- saline lake -- seasonal variation -- vertical distribution -- Russian Federation -- algae -- Arctodiaptomus -- Bacteria (microorganisms) -- Calanoida -- Chlorophyta -- Copepoda -- Crustacea -- Cyanobacteria -- Dictyosphaerium -- Lyngbya -- Lyngbya contorta
Аннотация: Data on the seasonal changes in vertical heterogeneity of the physical-chemical and biological parameters of the thermally stratified Shira Lake ecosystem (Khakasia, Siberia) in 1996-2000 have been analyzed. The interaction mechanisms involving: (1) The plankton populations in aerobic and anaerobic zones, involving the cycling of carbon and sulphur, (2) the primary production limitation (by light and phosphorus) and inhibition (by light), and (3) the kinetic characteristics of plankton populations have been elucidated. A mathematical model of the vertical structure of the lake's plankton populations, based on the ecosystem description and on vertical turbulent diffusion of the matter, has been constructed. The green alga Dictyosphaerium tetrachotomum (Chlorophyta) and the cyanobacterium Lyngbya contorta (Cyanophyta), which dominated the phytoplankton biomass, were taken as oxygen producers. Arctodiaptomus salinus (a calanoid copepod) has been assumed as the main grazer in Shira Lake as it dominated the zooplankton biomass. Four groups of microorganisms involved in the sulphur cycle formation have been distinguished: sulphur, sulphur purple, sulphur green and SRB. H2S is oxidized to sulphate (only the green sulphur bacteria oxidize it to sulphur), and sulphate is reduced to H2S, forming neither sulphur nor its water-soluble compounds. The role of grazing, light and nutrient limitation, in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. When the model takes into account both light limitation and nutrient limitation of algal growth by P and consumption of algae by crustaceans: (a) in the scenario where the P is formed only by the cycling and decomposition of autochthonous organic matter, both the green algae and cyanobacteria are eliminated; (b) in the scenario involving an additional P flux in the deep water layers the peak of the cyanobacteria is at a depth of 10 m, and its amplitude is close to the one observed in the lake. The position of the peak remains stable owing to the 'double' limitation mechanism: light 'from above' and P 'from below'. Another mechanism responsible for the deep position of the peak of cyanobacteria was analyzed mathematically based on the model involving the experimentally proven assumption of the growth inhibition by light in the epilimnion and the light limitation in the hypolimnion. The main result is: the peak is positioned stable at its depth and does not change with time. The analytical and numerical calculations made for this positioning mechanism yielded the formulae relating the depth of the maximum of algal biomass, the 'width' of the peak base and the peak amplitude and a number of parameters (algae elimination, turbulent diffusion coefficient, sedimentation rate, light extinction coefficient and light intensity). The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with field observations, e.g. for the different patterns for the peaks, and the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. For the first time, theoretically, based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been discriminated. The theoretical limit for the depth up to which the hydrogen-sulphide zone can ascend under the impact of allochthonous organic loading, has been determined.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Belolipetsky, V.M.; Zotina, T.A.; Gulati, R.D.

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13.

Interaction coefficients in the Lake Shira algal-bacterial community / V. V. Adamovich, T. A. Zotina, A. G. Degermendzhy // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P261-270, DOI 10.1023/A:1015638420766 . - ISSN 1386-2588
Кл.слова (ненормированные):
Bacterioplankton -- Feedback coefficient -- Influence coefficient -- Phytoplankton -- Specific growth rate -- bacterioplankton -- interspecific interaction -- microbial community -- phytoplankton -- quantitative analysis -- saline lake -- Russian Federation -- algae -- Bacteria (microorganisms)
Аннотация: A new method is proposed to evaluate the interaction coefficients in microbial communities interacting due to physical-chemical environmental factors. This method differs from the classical one suggested by E.P.Odum. Redefinition of the interaction coefficients allows the evaluation of the experimental (actual) and theoretical values of the coefficients for the hypothetical interaction layout. The interaction layout is considered to be a set of factors, the values of their transformation ratios and the form of dependence of a population's specific growth rate on these factors. A comparison of theoretical and experimental values of interaction coefficients enables us to assess the adequacy of the hypothetical interaction scheme in the microbial communities. The aim of the work is to test the suggested method on a natural algal-bacterial community of Shira Lake (Khakasia, Russia), which has been the object of detailed and concerted limnological studies and for which detailed data are available. Feedback coefficients of phyto- and bacterioplankton and the coefficients of influence of phytoplankton on bacterioplankton have been defined. Dominance of negative experimental IC values has been experimentally shown, which is indicative of the negative feedback in bacterio- and phytoplankton links of Shira Lake and of negative interpopulation (phytoplankton on bacterioplankton) interactions. The considerable differences observed between the experimental and theoretical coefficients indicates inadequacy of the assumed interaction layout of the community under study. Further investigations are needed to provide a precise sketch of the interactions.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Adamovich, V.V.; Zotina, T.A.; Degermendzhy, A.G.

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14.

Growth of dominant zooplankton species feeding on plankton microflora in Lake Shira / T. A. Temerova, A. P. Tolomeyev, A. G. Degermendzhy // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P235-243, DOI 10.1023/A:1015607304508 . - ISSN 1386-2588
Кл.слова (ненормированные):
Arctodiaptomus salinus -- Brachionus plicatilis -- Life history -- Reproduction -- Specific growth rate -- diet -- food limitation -- growth rate -- life history -- reproduction -- saline lake -- zooplankton -- Russian Federation -- algae -- Arctodiaptomus -- Bacteria (microorganisms) -- Brachionus -- Brachionus plicatilis -- Calanoida -- Copepoda -- Cyanobacteria -- Invertebrata -- Rotifera
Аннотация: Batch cultures and continuous flow cultures were used to study the growth rates of zooplankton species from Shira lake, the rotifer Brachionus plicatilis Muller and calanoid copepod Arctodiaptomus salinus Daday, which were fed on phytoplankton and bacterioplankton from the lake. Analyses of the birth and survival rates were used to demonstrate that the lake phytoplankton, consisting mostly of cyanobacteria and diatomaceous algae, is inadequotes for optimal realisation of the reproductive potential of B. plicatilis when compared with the bacterial diet. The study revealed that the kinetic growth characteristics of the two zooplankters were similar: B. plicatilis rmax, 0.120 d-1; S0, 0.253; and Ks, 0.114 mg dry mass l-1; and for A. salinus rmax, 0.129 d-1; S0, 0.240; and Ks, 0.171 mg dry mass l-1. Fluctuations in natural food concentration reduced the growth rate of both species. Even though the threshold concentration of food for B. plicatilis and A. salinus were quite similar, the copepods were less sensitive to food limitation.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Temerova, T.A.; Tolomeyev, A.P.; Degermendzhy, A.G.

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15.

Distribution of Gammarus lacustris Sars (Amphipoda, Gammaridae) in Lake Shira (Khakasia, Siberia) and laboratory study of its growth characteristics / A. Y. Yemelyanova, T. A. Temerova, A. G. Degermendzhy // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P245-256, DOI 10.1023/A:1015624205389 . - ISSN 1386-2588
Кл.слова (ненормированные):
Biomass -- Fresh-water shrimp -- Gammarus lacustris -- Growth -- Numbers -- biomass -- growth rate -- saline lake -- spatial distribution -- zoobenthos -- Russian Federation -- Amphipoda -- cellular organisms -- Decapoda (Crustacea) -- Gammaridae -- Gammarus -- Gammarus lacustris -- Invertebrata
Аннотация: Spatial distribution of Gammarus lacustris in Lake Shira and growth of young specimens that feed on lake biota were studied. The amphipods have been shown to inhabit the littoral, sublittoral and the upper aphytal zones of the lake on stony-sandy soil and silted sand. The young and adult individuals stay apart. The young live in submerged or semi-submerged vegetation in the littoral, the adults in the sublittoral and upper aphytal zones. Maximum density of amphipods was encountered in the areas influenced by human activity. The feeding experiments revealed that the lake plankton is a more important food source for G. lacustris than any other food species. The specific growth rate measured was 0.039 d-1, with a length increment 0.095 mm d-1.

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Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Yemelyanova, A.Y.; Temerova, T.A.; Degermendzhy, A.G.

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16.

Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646 / T. G. Volova [et al.] // Bioresource Technology. - 2013. - Vol. 146. - P215-222, DOI 10.1016/j.biortech.2013.07.070 . - ISSN 0960-8524
Кл.слова (ненормированные):
Autotrophic synthesis -- Cupriavidus eutrophus -- Polyhydroxyalkanoates
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenckii Institute of Physics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kiselev, E.G.; Shishatskaya, E.I.; Zhila, N.O.; Boyandin, A.N.; Syrvacheva, D.A.; Vinogradova, O.N.; Kalacheva, G.S.; Vasiliev, A.D.; Peterson, I.V.

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17.

Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

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Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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18.

An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation / E. I. Shishatskaya [et al.] // Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - P73-80 . - ISSN 1815-445X
Кл.слова (ненормированные):
Degradable polymer matrices -- Degradation -- PHAs -- Polyhydroxyalkanoates -- Subcutaneous implantation -- Tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.

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Держатели документа:
Institute of Modern Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Massachusetts Institute of Technology, Cambridge, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Nikolaeva, E.D.; Goreva, A.V.; Brigham, C.J.; Volova, T.G.; Sinskey, A.J.

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19.

Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kutz IPPAS H-252 / N. O. Zhila, G. S. Kalacheva, T. G. Volova // Journal of Applied Phycology. - 2011. - Vol. 23, Is. 1. - P47-52, DOI 10.1007/s10811-010-9532-8 . - ISSN 0921-8971
Кл.слова (ненормированные):
Botryococcus -- Fatty acid composition -- Lipid content -- Salinity -- algae -- Botryococcus -- Botryococcus braunii
Аннотация: The effect of 0.3 and 0.7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, and fatty acid profile of the lipids of the alga Botryococcus braunii IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first 3 days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols, and considerable changes in the lipid fatty acid profile: decreases in polyenoic acid contents (from 68.34% to 29.38% and 12.8%) and proportions of long-chain saturated acids (from 0.53% to 5.3% and 14.13% of the total fatty acids) at 0.3 M NaCl and 0.7 M NaCl, respectively. In later phases of the culture, at 0.3 M NaCl, the content of polyenoic acids rose to the values characteristic of the active growth phase of this alga. At 0.7 M NaCl, the proportion of polyenoic acids grew less significantly, but biomass concentration and total nitrogen increased, similarly to the experiment with 0.3 M NaCl. В© 2010 Springer Science+Business Media B.V.

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Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhila, N.O.; Kalacheva, G.S.; Volova, T.G.

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20.

Distribution and resorption of polymeric microparticles in visceral organs of laboratory animals after intravenous injection / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2009. - Vol. 148, Is. 5. - P789-793, DOI 10.1007/s10517-010-0817-3 . - ISSN 0007-4888
Кл.слова (ненормированные):
14C -- Intravenous injection -- Microparticles -- Resorbable polyhydroxybutyrate -- Visceral tissues -- carbon 14 -- microsphere -- polyhydroxyaminobutyric acid -- polymer -- unclassified drug -- animal experiment -- animal tissue -- article -- biocompatibility -- biodegradability -- body growth -- controlled drug release -- controlled study -- degradation kinetics -- drug delivery system -- drug dosage form -- experimental animal -- female -- liver parenchyma -- molecular weight -- nonhuman -- particle size -- physical development -- radioactivity -- rat -- spleen -- tissue distribution -- tissue structure -- Animals -- Animals, Laboratory -- Carbon Radioisotopes -- Female -- Injections, Intravenous -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Viscera -- Animalia
Аннотация: Microparticles obtained by using 14C-labeled resorbable hydroxyaminobutyric acid polymer were injected into the caudal vein of laboratory animals without negative aftereffects for their growth and development and without changes in the macro- and microstructure of organs and tissues. The distribution of microparticles in the viscera and the dynamics of accumulation of carbon-containing polymer degradation products in the viscera were studied. The main targets for the particles are liver tissues, as well as renal and splenic tissues. The polymeric matrix of the microparticles is most actively destroyed in the spleen and liver. The presence of high-molecular- weight polymeric matrix in organs indicates the integrity of microparticles and the possibility of long-term (up to 12 weeks) functioning of polymeric particles in vivo. В© 2009 Springer Science+Business Media, Inc.

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Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Basic Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Goreva, A.V.; Voinova, O.N.; Kalacheva, G.S.; Volova, T.G.

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