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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova, Marina D., Markova, Svetlana, V, Tikunova, Nina, V, Vysotski, Eugene S.
Заглавие : The Smallest Isoform ofMetridia longaLuciferase as a Fusion Partner for Hybrid Proteins
Колич.характеристики :16 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-242001]; Krasnoyarsk Regional Fund of Science; ICBFM SB RAS [AAAA-A17-117020210027-9]; Government of Krasnoyarsk Territory
Место публикации : Int. J. Mol. Sci.: MDPI, 2020. - Vol. 21, Is. 14. - Ст.4971. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms21144971
Примечания : Cited References:49. - The reported study was funded by the Russian Foundation for Basic Research (No. 18-44-242001), Government of Krasnoyarsk Territory, Krasnoyarsk Regional Fund of Science (S.V.M, M.D.L., and E.S.V.) and by the Russian State funded budget project of ICBFM SB RAS No. AAAA-A17-117020210027-9 (N.V.T.).
Предметные рубрики: COELENTERAZINE-BINDING PROTEIN
BIOLUMINESCENT REPORTER
GAUSSIA
Аннотация: Bioluminescent proteins are widely used as reporter molecules in various in vitro and in vivo assays. The smallest isoform of Metridia luciferase (MLuc7) is a highly active, naturally secreted enzyme which, along with other luciferase isoforms, is responsible for the bright bioluminescence of marine copepodMetridia longa. In this study, we report the construction of two variants of a hybrid protein consisting of MLuc7 and 14D5a single-chain antibody to the surface glycoprotein E of tick-borne encephalitis virus as a model fusion partner. We demonstrate that, whereas fusion of a single-chain antibody to either N- or C-terminus of MLuc7 does not affect its bioluminescence properties, the binding site on the single-chain antibody influences its binding capacity. The affinity of 14D5a-MLuc7 hybrid protein (K-D= 36.2 nM) where the C-terminus of the single-chain antibody was fused to the N-terminus of MLuc7, appeared to be 2.5-fold higher than that of the reverse, MLuc7-14D5a (K-D= 87.6 nM). The detection limit of 14D5a-MLuc7 hybrid protein was estimated to be 45 pg of the recombinant glycoprotein E. Although the smallest isoform ofM. longaluciferase was tested as a fusion partner only with a single-chain antibody, it is reasonable to suppose that MLuc7 can also be successfully used as a partner for genetic fusion with other proteins.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova M. D., Markova S. V., Tikunova N. V., Vysotski E. S.
Заглавие : The smallest isoform of Metridia longa luciferase as a fusion partner for hybrid proteins
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2020. - Vol. 21, Is. 14. - Ст.4971. - С. 1-16. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21144971
Аннотация: Bioluminescent proteins are widely used as reporter molecules in various in vitro and in vivo assays. The smallest isoform of Metridia luciferase (MLuc7) is a highly active, naturally secreted enzyme which, along with other luciferase isoforms, is responsible for the bright bioluminescence of marine copepod Metridia longa. In this study, we report the construction of two variants of a hybrid protein consisting of MLuc7 and 14D5a single-chain antibody to the surface glycoprotein E of tick-borne encephalitis virus as a model fusion partner. We demonstrate that, whereas fusion of a single-chain antibody to either N-or C-terminus of MLuc7 does not affect its bioluminescence properties, the binding site on the single-chain antibody influences its binding capacity. The affinity of 14D5a-MLuc7 hybrid protein (KD = 36.2 nM) where the C-terminus of the single-chain antibody was fused to the N-terminus of MLuc7, appeared to be 2.5-fold higher than that of the reverse, MLuc7-14D5a (KD = 87.6 nM). The detection limit of 14D5a-MLuc7 hybrid protein was estimated to be 45 pg of the recombinant glycoprotein E. Although the smallest isoform of M. longa luciferase was tested as a fusion partner only with a single-chain antibody, it is reasonable to suppose that MLuc7 can also be successfully used as a partner for genetic fusion with other proteins. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : The method to compare nucleotide sequences based on the minimum entropy principle
Колич.характеристики :14 с
Место публикации : Bull. Math. Biol.: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2003. - Vol. 65, Is. 2. - P309-322. - ISSN 0092-8240, DOI 10.1016/S0092-8240(02)00107-6
Примечания : Cited References: 20
Предметные рубрики: GENOME
Аннотация: A new method to compare two (or several) symbol sequences is developed. The method is based on the comparison of the frequencies of the small fragments of the compared sequences; it requires neither string editing, nor other transformations of the compared objects. The comparison is executed through a calculation of the specific entropy of a frequency dictionary against the special dictionary called the hybrid one; this latter is the statistical ancestor of the group of sequences under comparison. Some applications of the developed method in the fields of genetics and bioinformatics are discussed. (C) 2003 Society for Mathematical Biology. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya V. V., Bashmakova E. E., Kudryavtsev A. N., Vorobjeva M. A., Shatunova E. A., Frank L. A.
Заглавие : The Hybrid Protein ZZ-OL as an Analytical Tool for Biotechnology Research
Колич.характеристики :7 с
Коллективы : Russian FederationRussian Federation [MK-772.2020.4]; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10296]
Место публикации : Russ. J. Bioorg. Chem.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 46, Is. 6. - С. 1004-1010. - ISSN 1068-1620, DOI 10.1134/S106816202006014X. - ISSN 1608-330X(eISSN)
Примечания : Cited References:14. - The study was partially supported by a grant of the President of the Russian Federation for Young Scientists, the Candidates of Sciences (project MK-772.2020.4 in the part involving the synthesis and analysis of variants of proteins with melanoma-inhibiting activity) and a grant of the Russian Science Foundation (project no. 16-14-10296 in the part involving the bioluminescence analysis of binding of DNA aptamers to targets).
Аннотация: The gene of the hybrid protein that encodes the double synthetic fragment proZZ of the immunoglobulin-binding domain of protein A of Staphylococcus aureus and apo-obelin joined by a short linker has been cloned. The corresponding hybrid protein has been obtained by expression in Escherichia coli cells. The protein activated with a substrate (coelenterazine) possesses the bioluminescent Ca2+-dependent activity of the photoprotein close to that of recombinant wild-type obelin, and the immunoglobulin-binding ability of protein A. It has been shown that the hybrid can be used as a highly sensitive label to detect antibodies and estimate their affinity and interaction with recombinant proteins, as well as in investigations of other kinds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya V. V., Davydova A. S., Vorobjeva M. A., Frank L. A.
Заглавие : The Ca2+-Regulated Photoprotein Obelin as a Target for the RNA Aptamer Selection
Место публикации : Russ. J. Bioorg. Chem.: Pleiades Publishing, 2018. - Vol. 44, Is. 3. - С. 296-301. - ISSN 10681620 (ISSN) , DOI 10.1134/S1068162018030093
Ключевые слова (''Своб.индексиров.''): 2'-fluoro-rna--in vitro selection--rna aptamers--са2+-regulated photoprotein obelin
Аннотация: A variant of the Ca2+-regulated photoprotein obelin elongated with a hexahistidine peptide from the N-terminus was developed and studied. After immobilization on a metal-affine sorbent, the hybrid protein was applied as a target for the in vitro selection of RNA aptamers. According to the data of bioluminescent solid-phase microanalysis, the selection was shown to enrich the RNA library with obelin-affine molecules. © 2018, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chen, Yu-Hsiang, Chiang, Pei-Wen, Rogozin, Denis Yu, Degermendzhy, Andrey G., Chiu, Hsiu-Hui, Tang, Sen-Lin
Заглавие : Salvaging high-quality genomes of microbial species from a meromictic lake using a hybrid sequencing approach
Колич.характеристики :12 с
Коллективы : Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project [NSC 102-2923-B-001-004-MY3]; Russian Foundation for Basic Research GrantRussian Foundation for Basic Research (RFBR) [21-54-52001, MOST 105-2923-B-001-001-MY3]; Taiwan International Graduate Program (TIGP)
Место публикации : Commun. Biol.: NATURE PORTFOLIO, 2021. - Vol. 4, Is. 1. - Ст.996. - ISSN 2399-3642(eISSN), DOI 10.1038/s42003-021-02510-6
Примечания : Cited References:99. - Y This work was supported by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC 102-2923-B-001-004-MY3 and Russian Foundation for Basic Research Grant 21-54-52001 and MOST 105-2923-B-001-001-MY3. Y.H.C. would like to acknowledge the Taiwan International Graduate Program (TIGP) for its fellowship towards his graduate studies. We would like to thank Noah Last of Third Draft Editing for his English language editing.
Предметные рубрики: DIMETHYL SULFIDE
SULFUR BACTERIUM
SP-NOV.
CHLAMYDIALES
METAGENOME
Аннотация: Most of Earth's bacteria have yet to be cultivated. The metabolic and functional potentials of these uncultivated microorganisms thus remain mysterious, and the metagenome-assembled genome (MAG) approach is the most robust method for uncovering these potentials. However, MAGs discovered by conventional metagenomic assembly and binning are usually highly fragmented genomes with heterogeneous sequence contamination. In this study, we combined Illumina and Nanopore data to develop a new workflow to reconstruct 233 MAGs-six novel bacterial orders, 20 families, 66 genera, and 154 species-from Lake Shunet, a secluded meromictic lake in Siberia. With our workflow, the average N50 of reconstructed MAGs greatly increased 10-40-fold compared to when the conventional Illumina assembly and binning method were used. More importantly, six complete MAGs were recovered from our datasets. The recovery of 154 novel species MAGs from a rarely explored lake greatly expands the current bacterial genome encyclopedia. Chen and colleagues develop a workflow for assembling high quality metagenome-associated genomes for microbial species using long and short reads, in this case from a meromictic lake. A full, detailed workflow is provided in for use by the community.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shumilova A. A., Myltygashev M. P., Kirichenko A. K., Nikolaeva E. D., Volova T. G., Shishatskaya E. I.
Заглавие : Porous 3D implants of degradable poly-3-hydroxybutyrate used to enhance regeneration of rat cranial defect
Колич.характеристики :12 с
Коллективы : Russian Academy of Sciences [01201351505]
Место публикации : J. Biomed. Mater. Res. Part A: WILEY-BLACKWELL, 2017. - Vol. 105, Is. 2. - С. 566-577. - ISSN 1549-3296, DOI 10.1002/jbm.a.35933. - ISSN 1552-4965(eISSN)
Примечания : Cited References:53. - Contract grant sponsor: The state budget allocated to the fundamental research at the Russian Academy of Sciences; contract grant number: 01201351505
Предметные рубрики: BONE TISSUE REGENERATION
CRITICAL SIZE DEFECT
IN-VITRO
CALVARIAL
Ключевые слова (''Своб.индексиров.''): 3d implants--poly-3-hydroxybutyrate--msc differentiation--grafts for--osteogenesis--regeneration of defect cranial
Аннотация: The study describes preparation and testing of porous 3D implants of natural degradable polymer of 3-hydroxybutyric acid P(3HB) for regeneration of bone tissue defects. The ability of the P(3HB) implants to favor attachment and facilitate proliferation and directed differentiation of mesenchymal stem cells (MSCs) was studied in the culture of MSCs isolated from bone marrow and adipose tissue. Tissue-engineered hybrid systems (grafts) constructed using P(3HB) and P(3HB) in combination with osteoblasts were used in experiments on laboratory animals (n=48) with bone defect model. The defect model (5 mm in diameter) was created in the rat parietal bone, and filling of the defect by the new bone tissue was monitored in the groups of animals with P(3HB) implants, with commercial material, and without implants (negative control). Computed tomography (CT) and histologic examination showed that after 120 days, in the group with the osteoblast-seeded P(3HB) implants, the defect was completely closed; in the group with the cell-free P(3HB) implants, the remaining defect was no more than 10% of the initial one (0.5 mm); in both the negative and positive controls, the size of the defect was about 1.0-1.2 mm. These results suggest that P(3HB) has good potential as osteoplastic material for reconstructive osteogenesis. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 566-577, 2017.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova, Eugenia E., Panamarev, Nikita S., Kudryavtsev, Alexander N., Frank, Ludmila A.
Заглавие : N-extended photoprotein obelin to competitively detect small protein tumor markers
Колич.характеристики :5 с
Коллективы : Russian Federation for young scientists [MK-772.2020.4]; Government of Krasnoyarsk Territory, Krasnoyarsk Regional Science Foundation [2021012006966]
Место публикации : Biochem. Biophys. Res. Commun.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2022. - Vol. 598. - С. 69-73. - ISSN 0006-291X, DOI 10.1016/j.bbrc.2022.02.011. - ISSN 1090-2104(eISSN)
Примечания : Cited References:15. - The work was partially supported by a grant of the President of the Russian Federation for young scientists, the candidates of sciences (project MK-772.2020.4, study of a hybrid protein with melanoma-inhibiting activity) and Government of Krasnoyarsk Territory, Krasnoyarsk Regional Science Foundation (project No 2021012006966, study of a hybrid with protein survivin).
Предметные рубрики: MELANOMA INHIBITORY-ACTIVITY
SURVIVIN
Аннотация: Two variants of Ca2+-regulated photoprotein obelin, extended from the N-terminus with small tumor markers-melanoma inhibitory activity protein (MIA) and survivin, one of the protein inhibitors of apoptosis, were designed, obtained and studied. Both domains in the obtained hybrid proteins exhibit the properties of the initial molecules: the main features of Ca2+-triggered bioluminescence are close to those of obelin, and the tumor markers' domains are recognized and bound by the corresponding antibodies. The obtained hybrids compete with the corresponding tumor markers for binding with antibodies, immobilized on the surface and their use has been shown to be promising as bioluminescent labels in a one-stage solid-phase competitive immunoassay. (c) 2022 Published by Elsevier Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ranjan, Rajeev, Esimbekova, Elena N., Kirillova, Maria A., Kratasyuk, Valentina A.
Заглавие : Metal-enhanced luminescence: Current trend and future perspectives- A review
Колич.характеристики :13 с
Коллективы : Russian Foundation for Basic Research [16-34-60100, 16-44-242126]; Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund; [01201351504]
Место публикации : Anal. Chim. Acta: ELSEVIER SCIENCE BV, 2017. - Vol. 971. - С. 1-13. - ISSN 0003-2670, DOI 10.1016/j.aca.2017.03.051. - ISSN 1873-4324(eISSN)
Примечания : Cited References:124. - The research was partially supported by the Russian Foundation for Basic Research [project No 16-34-60100], the state budget allocated to the fundamental research [project No 01201351504] and Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund to the research project and Russian Foundation for Basic Research [project No 16-44-242126].
Предметные рубрики: RESONANCE ENERGY-TRANSFER
PLASMON-COUPLED EMISSION
Ключевые слова (''Своб.индексиров.''): nanomaterials--surface plasmons--luminescence enhancement--biosensors
Аннотация: Optically enhanced biosensing strategies are prerequisites for developing miniature and highly sensitive multiplexed analytical platforms. Such smart biosensing systems are highly promising for use in the fields of biomedicine and environmental monitoring. Optical signal enhancement during bioassays is attributed to the complex opto-electronic interactions of incoming photonic signals at the nanomaterial interface. Research on the use of metals other than gold and silver for such purposes tends to extend the spectral window to observe luminescence enhancement effects. Such manifold increase in luminescence may be explained by the principles of plasmon coupling, directional emission led high collection efficiency, Rayleigh scattering and related opto-electronic events. The present review begins with a mechanistic description of important phenomena associated with metal-induced luminescence enhancement, particularly focusing on the origin of metal-enhanced luminescence. This review further analyses the hybrid nanostructure capabilities responsible for maintaining unique opto-electronic properties during bio-functionalisation. Current research trends in this area, future scope of this field for designing useful bioassays and concluding remarks are then discussed. (C) 2017 Elsevier B.V. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova E. E., Krasitskaya V. V., Kudryavtsev A. N., Grigorenko V. G., Frank L. A.
Заглавие : Hybrid Minimal Core Streptavidin–Obelin as a Versatile Reporter for Bioluminescence-based Bioassay
Место публикации : Photochem. Photobiol.: Blackwell Publishing Inc., 2017. - Vol. 93, Is. 2. - С. 548-552. - ISSN 00318655 (ISSN) , DOI 10.1111/php.12648
Аннотация: Ca2+-regulated photoprotein obelin was genetically fused with a minimum-sized core streptavidin. Hybrid protein (SAV–OL) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid-phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV–OL has been shown to be a much more sensitive label than the chemical conjugate of a full-length streptavidin with obelin. © 2016 The American Society of Photobiology
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chen S. -F., Vysotski E. S., Liu Y. -J.
Заглавие : H2O-Bridged Proton-Transfer Channel in Emitter Species Formation in Obelin Bioluminescence
Место публикации : J Phys Chem B: American Chemical Society, 2021. - Article in press. - ISSN 15206106 (ISSN), DOI 10.1021/acs.jpcb.1c03985
Аннотация: Bioluminescence of a number of marine organisms is conditioned by Ca2+-regulated photoprotein (CaRP) with coelenterazine as the reaction substrate. The reaction product, coelenteramide, at the first singlet excited state (S1) is the emitter of CaRP. The S1-state coelenteramide is produced via the decomposition of coelenterazine dioxetanone. Experiments suggested that the neutral S1-coelenteramide is the primary emitter species. This supposition contradicts with theoretical calculations showing that the anionic S1-coelenteramide is a primary product of the decomposition of coelenterazine dioxetanone. In this study, applying molecular dynamic (MD) simulations and the hybrid quantum mechanics/molecular mechanics (QM/MM) method, we investigated a proton-transfer (PT) process taking place in CaRP obelin from Obelia longissima for emitter formation. Our calculations demonstrate a concerted PT process with a water molecule as a bridge between anionic S1-coelenteramide and the nearest histidine residue. The low activation barrier as well as the strong hydrogen-bond network between the proton donor and the proton acceptor suggests a fast PT process comparable with that of the lifetime of excited anionic S1-coelenteramide. The existence of the PT process eliminates the discrepancy between experimental and theoretical studies. The fast PT process at emitter formation can also take place in other CaRPs. © 2021 American Chemical Society.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chen, Shu-Feng, Vysotski, Eugene S., Liu, Ya-Jun
Заглавие : H2O-Bridged Proton-Transfer Channel in Emitter Species Formation in Obelin Bioluminescence
Колич.характеристики :7 с
Коллективы : Program of Shanghai Institute of Technology [YJ2016-42]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21973005, 21911530094]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00085, 19-14-53004]
Место публикации : J. Phys. Chem. B: AMER CHEMICAL SOC, 2021. - Vol. 125, Is. 37. - С. 10452-10458. - ISSN 1520-6106, DOI 10.1021/acs.jpcb.1c03985. - ISSN 1520-5207(eISSN)
Примечания : Cited References:50. - This work was supported by the Program of Shanghai Institute of Technology (no. YJ2016-42), the National Natural Science Foundation of China (21973005 and 21911530094), and the Russian Foundation for Basic Research (20-04-00085 and 19-14-53004).
Предметные рубрики: CHEMILUMINESCENT DECOMPOSITION
FLUORESCENCE-SPECTRA
MECHANISM
QM/MM
Аннотация: Bioluminescence of a number of marine organisms is conditioned by Ca2+-regulated photoprotein (CaRP) with coelenterazine as the reaction substrate. The reaction product, coelenteramide, at the first singlet excited state (S-1) is the emitter of CaRP. The S-1-state coelenteramide is produced via the decomposition of coelenterazine dioxetanone. Experiments suggested that the neutral S-1-coelenteramide is the primary emitter species. This supposition contradicts with theoretical calculations showing that the anionic S-1-coelenteramide is a primary product of the decomposition of coelenterazine dioxetanone. In this study, applying molecular dynamic (MD) simulations and the hybrid quantum mechanics/molecular mechanics (QM/MM) method, we investigated a proton-transfer (PT) process taking place in CaRP obelin from Obelia longissima for emitter formation. Our calculations demonstrate a concerted PT process with a water molecule as a bridge between anionic S-1-coelenteramide and the nearest histidine residue. The low activation barrier as well as the strong hydrogen-bond network between the proton donor and the proton acceptor suggests a fast PT process comparable with that of the lifetime of excited anionic S-1-coelenteramide. The existence of the PT process eliminates the discrepancy between experimental and theoretical studies. The fast PT process at emitter formation can also take place in other CaRPs.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Fedoseeva, Yu. V., Bulusheva L.G., Okotrub A.V., Kanygin M.A., Gorodetskiy D.V., Asanov I.P., Vyalikh D.V., Puzyr A.P., Bondar V.S.
Заглавие : Field emission luminescence of nanodiamonds deposited on the aligned carbon nanotube array
Колич.характеристики :7 с
Коллективы : RFBR grant [13-03-12118], bilateral Program "Russian-German Laboratory at BESSY''
Место публикации : Sci Rep: NATURE PUBLISHING GROUP, 2015. - Vol. 5. - Ст.9379. - ISSN 2045-2322, DOI 10.1038/srep09379
Примечания : Cited References:49. - The work was supported by RFBR grant 13-03-12118 in the part of electroluminescence measurements and the bilateral Program "Russian-German Laboratory at BESSY''. We are grateful to Mr. A.V. Ischenko for the TEM measurements, Mr. S.I. Kozhemyachenko for the Raman spectra, Mrs. N.I. Alferova for the IR spectra, and Mr. D.V. Gulyaev for the photoluminescence spectra.
Предметные рубрики: DETONATION NANODIAMOND
ULTRANANOCRYSTALLINE DIAMOND
Аннотация: Detonation nanodiamonds (NDs) were deposited on the surface of aligned carbon nanotubes (CNTs) by immersing a CNT array in an aqueous suspension of NDs in dimethylsulfoxide (DMSO). The structure and electronic state of the obtained CNT-ND hybrid material were studied using optical and electron microscopy and Infrared, Raman, X-ray photoelectron and near-edge X-ray absorption fine structure spectroscopy. A non-covalent interaction between NDs and CNT and preservation of vertical orientation of CNTs in the hybrid were revealed. We showed that current-voltage characteristics of the CNT-ND cathode are changed depending on the applied field; below similar to 3 V/mu m they are similar to those of the initial CNT array and at the higher field they are close to the ND behavior. Involvement of the NDs in field emission process resulted in blue luminescence of the hybrid surface at an electric field higher than 3.5 V/mu m. Photoluminescence measurements showed that the NDs emit blue-green light, while blue luminescence prevails in the CNT-ND hybrid. The quenching of green luminescence was attributed to a partial removal of oxygen-containing groups from the ND surface as the result of the hybrid synthesis.
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14.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Mezhevikin V.V., Okhonin V.A.
Заглавие : Evaluation of optimal configuration of hybrid life support system for space
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: SPACE LIFE SUPPORT SYSTEMS AND THE LUNAR FARSIDE CRATER SAHA PROPOSAL. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 2000. - Vol. 26: F4 4 F4 5 and F3 7 Symposia of COSPAR Scientific Commission F Held at the 32nd COSPAR Scientific Assembly (JUL 12-19, 1998, NAGOYA, JAPAN), Is. 2. - P323-326. - ISBN 0273-1177, DOI 10.1016/S0273-1177(99)01071-6
Примечания : Cited References: 12
Аннотация: Any comprehensive evaluation of Life Support Systems (LSS) for space applications has to be conducted taking into account not only mass of LSS components but also all relevant equipment and storage: spare parts, additional mass of space ship walls, power supply and heat rejection systems. In this paper different combinations of hybrid LSS (HLSS) components were evaluated. Three variants of power supply were under consideration - solar arrays, direct solar light transmission to plants, and nuclear power. The software based on simplex approach was used for optimizing LSS configuration with respect to its mass. It was shown that there are several LSS configuration, which are optimal for different time intervals. Optimal configurations of physical-chemical (P/C), biological and hybrid LSS for three types of power supply are presented. (C) 2000 COSPAR. Published by Elsevier Science Ltd.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeev A.V., Setkov N.A.
Заглавие : DNA synthesis in heterokaryons obtained by fusion of hepatocytes from a regenerating mouse liver and NIH 3T3 fibroblasts
Место публикации : Doklady Biological Sciences. - 2000. - Vol. 372, Is. 1-6. - P329-332. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): dna--thymidine--animal--article--biosynthesis--cell division--cell fusion--cell strain 3t3--cytology--hybrid cell--kinetics--liver cell--liver regeneration--metabolism--mouse--3t3 cells--animals--cell division--cell fusion--dna--hepatocytes--hybrid cells--kinetics--liver regeneration--mice--thymidine
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : Comparison of symbol sequences: No editing, no alignment
Колич.характеристики :18 с
Место публикации : Open Syst. Inf. Dyn.: KLUWER ACADEMIC PUBL, 2002. - Vol. 9, Is. 1. - P19-36. - ISSN 1230-1612, DOI 10.1023/A:1014278811727
Примечания : Cited References: 12
Предметные рубрики: MULTIPLE
Аннотация: The new method to compare two (or several) symbol sequences is developed. The method is based on the comparison of the frequencies of the small fragments of the compared sequences; it requires no string editing or other transformations of the compared objects. The comparison is provided through a calculation of the specific entropy of a frequency dictionary against the special dictionary called the hybrid one; the latter is the statistical ancestor of the group of sequences to be compared. Some applications of the method developed to genetics, bioinformatics, and linguistics axe discussed.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Nikolaeva E. D., Kirichenko A. K., Shishatskaya E. I.
Заглавие : Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 230-240. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.068
Примечания : Cited By :1
Ключевые слова (''Своб.индексиров.''): bacterial cellulose--composites--polyhydroxyalkanoates--3 hydroxybutyric acid 4 hydroxybutyric acid copolymer--actovegin--bacterial cellulose--cellulose--collagen type 1--copolymer--cytokeratin 10--cytokeratin 14--solcoseryl--unclassified drug--angiogenesis--animal cell--animal experiment--animal model--animal tissue--biochemical analysis--biocompatibility--burn--cell growth--controlled study--cutaneous parameters--differential scanning calorimetry--drug release--elemental analysis--female--fibroblast--histopathology--in vitro study--in vivo study--inflammation--mechanics--nonhuman--rat--review--wound healing--wound planimetry--x ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova, Ludmila P., Kudryavtsev, Alexander N., Stepanyuk, Galina A., Baykov, Ivan K., Morozova, Vera V., Tikunova, Nina V., Dubova, Maria A., Lyapustin, Victor N., Yakimenko, Valeri V., Frank, Ludmila A.
Заглавие : Bioluminescent detection probe for tick-borne encephalitis virus immunoassay
Колич.характеристики :7 с
Коллективы : Russian Academy of Sciences, Siberian Branch [139], Russian Academy of Sciences [VI 57.1.1]
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2015. - Vol. 407, Is. 18. - С. 5417-5423. - ISSN 1618-2642, DOI 10.1007/s00216-015-8710-6. - ISSN 1618-2650(eISSN)
Примечания : Cited References:19. - The work was supported by the Russian Academy of Sciences, Siberian Branch, within the framework of the Interdisciplinary Integration Project No. 139 and the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No. VI 57.1.1).
Предметные рубрики: COELENTERAZINE-BINDING PROTEIN
ENZYME-IMMUNOASSAY
RENILLA-MUELLERI
Ключевые слова (''Своб.индексиров.''): tick-borne encephalitis virus--single-chain antibody--luciferase--immunoassay
Аннотация: To facilitate the detection of the tick-borne encephalitis virus (TBEV), the causative agent of one of the most severe human neuroinfections, we have developed an immunoassay based on bioluminescent hybrid protein 14D5a-Rm7 as a detection probe. The protein containing Renilla luciferase as a reporter and a single-chain variable fragment (scFv) of murine immunoglobulin to TBEV as a recognition element was constructed, produced by bacterial expression, purified, and tested. Both domains were shown to reveal their specific biological properties-affinity to the target antigen and bioluminescent activity. Hybrid protein was applied as a label for solid-phase immunoassay of the antigens, associated with the tick-borne encephalitis virus (native glycoprotein E or extracts of the infected strain of lab ticks). The assay demonstrates high sensitivity (0.056 ng of glycoprotein E; 10(4)-10(5) virus particles or 0.1 pg virions) and simplicity and is competitive with conventional methods for detection of TBEV.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryavtsev A. N., Burakova L. P., Frank L. A.
Заглавие : Bioluminescent detection of tick-borne encephalitis virus in native ticks
Место публикации : Anal. Methods: Royal Society of Chemistry, 2017. - Vol. 9, Is. 15. - С. 2252-2255. - ISSN 17599660 (ISSN) , DOI 10.1039/c7ay00535k
Ключевые слова (''Своб.индексиров.''): proteins--recombinant proteins--viruses--binding proteins--bioluminescence
Аннотация: A one-step bioluminescent immunoassay for tick-borne encephalitis virus (TBEV) in natural ticks based on the hybrid protein 14D5a-Rm7 was developed. Recombinant Ca2+-dependent coelenterazine-binding protein was shown to be a more convenient substrate form for the Rm7 domain than coelenterazine. Over 600 samples of natural ticks were analyzed and shown to have essential differences in the discrimination factor D for TBEV-positive (2.77 ± 0.81) and TBEV-negative (1.15 ± 0.28) samples. © The Royal Society of Chemistry.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I., Bartsev S.I., Mezhevikin V.V., Okhonin V.A.
Заглавие : An alternative approach to solar system exploration providing safety of human mission to Mars
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 1. - С. 17-24. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(02)00657-9
Ключевые слова (''Своб.индексиров.''): health care--parameter estimation--planets--radiation--safety factor--mars exploration--solar system--space technology--electricity--feasibility studies--human engineering--humans--life support systems--man-machine systems--mars--radiation protection--robotics--safety--solar system--space flight--spacecraft
Аннотация: For systematic human Mars exploration, meeting crew safety requirements, it seems perspective to assemble into a spacecraft: an electrical rocket, a well-shielded long-term life support system, and a manipulator-robots operating in combined "presence effect" and "master-slave" mode. The electrical spacecraft would carry humans to the orbit of Mars, providing short distance (and low signal time delay) between operator and robot-manipulators, which are landed on the surface of the planet. Long-term hybrid biological and physical/chemical LSS could provide environment supporting human health and well being. Robot-manipulators operating in "presence effect" and "master-slave" mode exclude necessity of human landing on Martian surface decreasing the level of risk for crew. Since crewmen would not have direct contact with the Martian environment then the problem of mutual biological protection is essentially reduced. Lightweight robot-manipulators, without heavy life support systems and without the necessity of returning to the mother vessel, could be sent as scouts to different places on the planet surface, scanning the most interesting for exobiological research site. Some approximate estimations of electric spacecraft, long-term hybrid LSS, radiation protection and mission parameters are conducted and discussed. В© 2002 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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