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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko, T. V., Shikhov, V. N., Tikhomirov, A. A.
Заглавие : Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves
Колич.характеристики :7 с
Коллективы : section VI of the Program for Basic Research of the State Academies of Sciences [56.1.4]
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 62, Is. 3. - С. 307-313. - ISSN 1021-4437, DOI 10.1134/S1021443715020144. - ISSN 1608-3407(eISSN)
Примечания : Cited References:30. - This work was supported by the State Assignment, topic no. 56.1.4, section VI of the Program for Basic Research of the State Academies of Sciences for 2013-2020.
Предметные рубрики: LEAF SENESCENCE
ONTOGENIC APPROACH
LIGHT DEPENDENCE
PARAMETERS
Ключевые слова (''Своб.индексиров.''): triticum sativus--chlorophyll fluorescence parameters--actinic light--intensity--leaf ontogeny
Аннотация: Wheat (Triticum sativus L.) seedlings of various ages (2- to 16-day-old plants) were used to study age-dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu- orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, controlled environmental conditions, and 690 A mu mol/(m(2) s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 A mu mol/(m(2) s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for measurements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the maximal photochemical quantum yield of photosystem II (F (v)/F (m)), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non-photochemical (qN and NPQ) quenching of chlorophyll fluorescence, the F (p)/F (t) ratio, and the "vitality index" (fluorescence decrease ratio, R (fd)). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 A mu mol/(m(2) s)), age-dependent changes in NPQ, F (p)/F (t), and R (fd) were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 A mu mol/(m(2) s) revealed that R (fd), NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A., Ushakova S., Tikhomirova N., Velichko V., Trifonov S., Anishchenko O.
Заглавие : Establishing cycling processes in an experimental model of a closed ecosystem
Место публикации :. - 2018. - ISSN 00945765 (ISSN) , DOI 10.1016/j.actaastro.2018.08.023
Ключевые слова (''Своб.индексиров.''): cycling--experimental model of the closed ecosystem--oxidation of human and plant wastes--plant productivity--carbon dioxide--cultivation--ecosystems--life support systems (spacecraft)--closed ecological systems--cycling--expanded clay aggregates--experimental modeling--photosynthetic activity--plant productivity--plant wastes--terrestrial application--plant shutdowns
Аннотация: The purpose of this study was to investigate mass exchange processes in the experimental model of a closed ecological system intended for an estimated portion of a human in the long-duration (several-month) experiment. The diversity of the vegetable crop community in the system was increased, human wastes were involved in mass exchange processes, and human respiration was periodically connected to the system. The system has been designed to test different prospective technologies for future closed life support systems intended for prolonged autonomous operation in space and terrestrial applications. Three methods of plant cultivation in the conveyer mode have been used: hydroponics on expanded clay aggregate, growing plants on the soil-like substrate, and plant cultivation in aquaculture. The technology of more effective oxidation of organic wastes in a physicochemical processing reactor has been developed. A human exhaled the air into the system and consumed the air from the system. O2 concentration did not drop below 20.8% and did not rise above 22.6%. CO2 concentration varied between 800 ppm and 2500 ppm. Plants growing under this CO2 range at a preset light irradiance showed optimal photosynthetic activity. The closure coefficients for Ca, Mg, S, N, K and P were above 90%. However, compared with the inflow, only 55% Ca, about 80% Mg, and 75% Na and P were removed from the system. The technological processes developed in this study will need to be modified and improved before they can be used in a full-scale closed biotechnical life support system intended for prolonged operation. © 2018 IAA
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov, Alexander, Ushakova, Sofya, Tikhomirova, Natalia, Velichko, Vladimir, Trifonov, Sergey, Anishchenko, Olesya
Заглавие : Establishing cycling processes in an experimental model of a closed ecosystem
Колич.характеристики :8 с
Коллективы : Russian Science Foundation, RussiaRussian Science Foundation (RSF) [14-14-00599Pi]
Место публикации : Acta Astronaut.: PERGAMON-ELSEVIER SCIENCE LTD, 2020. - Vol. 166: 21st International-Academy-of-Astronautics (IAA) Humans in Space (NOV 27-30, 2017, Shenzhen, PEOPLES R CHINA). - С. 537-544. - ISSN 0094-5765, DOI 10.1016/j.actaastro.2018.08.023. - ISSN 1879-2030(eISSN)
Примечания : Cited References:18. - The study was supported by the Russian Science Foundation, Russia (Project No. 14-14-00599 Pi) and carried out in the IBP SB RAS at FRCKRC SB RAS. No competing financial interests exist.
Предметные рубрики: BIOREGENERATIVE LIFE-SUPPORT
EXCHANGE
WASTES
MASS
Аннотация: The purpose of this study was to investigate mass exchange processes in the experimental model of a closed ecological system intended for an estimated portion of a human in the long-duration (several-month) experiment. The diversity of the vegetable crop community in the system was increased, human wastes were involved in mass exchange processes, and human respiration was periodically connected to the system. The system has been designed to test different prospective technologies for future closed life support systems intended for prolonged autonomous operation in space and terrestrial applications. Three methods of plant cultivation in the conveyer mode have been used: hydroponics on expanded clay aggregate, growing plants on the soil-like substrate, and plant cultivation in aquaculture. The technology of more effective oxidation of organic wastes in a physicochemical processing reactor has been developed. A human exhaled the air into the system and consumed the air from the system. O-2 concentration did not drop below 20.8% and did not rise above 22.6%. CO2 concentration varied between 800 ppm and 2500 ppm. Plants growing under this CO2 range at a preset light irradiance showed optimal photosynthetic activity. The closure coefficients for Ca, Mg, S, N, K and P were above 90%. However, compared with the inflow, only 55% Ca, about 80% Mg, and 75% Na and P were removed from the system. The technological processes developed in this study will need to be modified and improved before they can be used in a full-scale closed biotechnical life support system intended for prolonged operation.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Morozov Y. A., Trifonov S. V., Ushakova S. A., Anishchenko O. V., Tikhomirov A. A.
Заглавие : Feasibility of incorporating all products of human waste processing into material cycling in the BTLSS
Место публикации : Life Sci. Space Res.: Elsevier Ltd, 2018. - Vol. 18. - С. 29-34. - ISSN 22145524 (ISSN) , DOI 10.1016/j.lssr.2018.05.002
Ключевые слова (''Своб.индексиров.''): btlss--closure--cycling--hydroponics--nutrient availability--sediment
Аннотация: The present study addresses the ways to increase the closure of biotechnical life support systems (BTLSS) for space applications. A promising method of organic waste processing based on “wet combustion” in hydrogen peroxide developed at the IBP SB RAS to produce fertilizers for higher plants is discussed. The method is relatively compact, energy efficient, productive, and eco-friendly. However, about 4–6 g/L of recalcitrant sediment containing such essential nutrients as Ca, Mg, P, Fe, Cu, Mn, and Zn precipitates after the initial process. These elements are unavailable to plants grown hydroponically and, thus, drop out of the cycling as dead-end products. Possible methods of dissolving that sediment have been studied. Results of experiments show that the most promising method is additional oxidation of the sediment in HNO3 + H2O2. By using the new technological process, which only involves substances synthesized inside the BTLSS material flows, more than 90% of each nutrient can be converted into the form available to plants in irrigation solutions, thus returning them into the material cycling. The results obtained in this study show the efficacy of supplementing the irrigation solutions with the mineral nutrients after sediment dissolution. Lettuce plants grown as the test object on the newly prepared irrigation solutions produced the yield that was more than twice higher than the yield produced on the nutrient solutions prepared without the sediment conversion into a soluble form. Composition of the gases emitted during this process has been analyzed. Dynamics of oxidation of the small fractions of a wax-like sediment remaining after the initial sediment dissolution in HNO3 + H2O2 in the BTLSS soil-like substrate has been studied. The entire technological scheme aimed at the full inclusion of all human wastes into the BTLSS cycling has been suggested and discussed. A process scheme of including products of human waste processing in the biotic cycle of the BTLSS is discussed in the conclusion. © 2018 The Committee on Space Research (COSPAR)
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Morozov Ye. A., Trifonov S. V., Ushakova S. A., Anishchenko O. V., Tikhomirov A. A.
Заглавие : Feasibility of incorporating all products of human waste processing into material cycling in the btlss
Место публикации : Proceedings of the International Astronautical Congress, IAC. - 2017. - Vol. 4: 68th International Astronautical Congress: Unlocking Imagination, Fostering Innovation and Strengthening Security, IAC 2017 (25 September 2017 through 29 September 2017, ) Conference code: 136635. - С. 2143-2149
Ключевые слова (''Своб.индексиров.''): btlss--closure--cycling--hydroponics--nutrient availability--sediment--dissolution--energy efficiency--irrigation--life support systems (spacecraft)--plant shutdowns--sediments--space applications--waste incineration--btlss--closure--cycling--hydroponics--nutrient availability--nutrients
Аннотация: The present study addresses the ways to increase the closure of biotechnical life support systems (BTLSS) for space applications. A promising method of organic waste processing based on wet combustion in hydrogen peroxide developed at the IBP SB RAS to produce fertilizers for higher plants is discussed. The method is relatively compact, energy efficient, productive, and eco-friendly. However, about 4-6 g/L of recalcitrant sediment containing such essential nutrients as Ca, Mg, P, Fe, Cu, Mn, and Zn precipitates after the initial process. These elements are unavailable to plants grown hydroponically, thus dropping out of the cycling as deadlock products. Possible methods of dissolving that sediment have been studied. Results of experiments show that the most promising method is additional oxidation of the sediment in HNO3 + H2O2. By using the new technological process, which only involves substances synthesized inside the BTLSS material flows, more than 90% of each nutrient can be converted into the available form in irrigation solutions, thus returning them into the material cycling. The efficiency of irrigation solutions beneficiated with the mineral nutrients after the sediment dissolution has been shown. Lettuce plants grown as the test object on the newly prepared irrigation solutions produced the yield that was higher more than twice compared to the nutrient solutions prepared without the sediment conversion into a soluble state. Composition of the gases emitted during this process has been analysed. Dynamics of oxidation of small fractions of a wax-like sediment remaining after its dissolution in the BTLSS soil-like substrate has been studied. In conclusion, the entire technological chain aimed at inclusion of deadlock products of human waste wet combustion into the BTLSS cycling has been suggested and discussed. © 2017 by the International Astronautical Federation (IAF). All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I., Tirranen L.S., Borodina E.V., Rygalov V.Ye.
Заглавие : Impaired growth of plants cultivated in a closed system: Possible reasons
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 1927-1930. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): air conditioning--air pollutant--article--culture medium--growth, development and aging--human--hydroponics--methodology--microbiology--microclimate--plant--sewage--wheat--air conditioning--air pollutants--culture media--ecological systems, closed--environment, controlled--humans--hydroponics--life support systems--plants--triticum--waste disposal, fluid--water microbiology
Аннотация: Plants in experiments on "man-higher plants" closed ecosystem (CES) have been demonstrated to have inhibited growth and reduced productivity due to three basic factors: prolonged usage of a permanent nutrient solution introduction into the nutrient medium of intra-system gray water, and closure of the system. Gray water was detrimental to plants the longer the nutrient solution was used. However, higher plant growth was mostly affected by the gaseous composition of the CES atmosphere, through accumulation of volatile substances. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S., Tikhomirov A., Shikhov V., Kudenko Yu., Anischenko O., Gros J.-B., Lasseur Ch.
Заглавие : Increased BLSS closure using mineralized human waste in plant cultivation on a neutral substrate
Место публикации : Advances in Space Research. - 2009. - Vol. 44, Is. 8. - С. 971-978. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2009.06.003
Ключевые слова (''Своб.индексиров.''): biological life support system--crops--human waste recycling--hydroponics--biological life support system--biological life support systems--biological productivity--control experiments--control plants--environmental conditions--expanded clay--human waste--human waste recycling--hydroponics--in-plants--macro element--mineral element--neutral solution--nutrient solution--photosynthetic apparatus--pisum sativum--plant growth--scale potential--spring wheat--triticum aestivum--vegetation periods--water extracts--wheat straws--clay minerals--crops--minerals--mining--nutrients--pilot plants--plant life extension--potassium--productivity--recycling--solvent extraction--vegetation--water content--plant shutdowns
Аннотация: The purpose of this work was to study the full-scale potential use of human mineralized waste (feces and urine) as a source of mineral elements for plant cultivation in a biological life support system (BLSS). Plants that are potential candidates for a photosynthesizing link were grown on a neutral solution containing human mineralized waste. Spring wheat Triticum aestivum L., peas Pisum sativum L. Ambrosia cultivar and leaf lettuce Lactuca sativa L., Vitaminny variety, were used. The plants were grown hydroponically on expanded clay aggregates in a vegetation chamber in constant environmental conditions. During plant growth, a determined amount of human mineralized waste was added daily to the nutrient solution. The nutrient solution remained unchanged throughout the vegetation period. Estimated plant requirements for macro-elements were based on a total biological productivity of 0.04 kg day-1 m-2. As the plant requirements for potassium exceeded the potassium content of human waste, a water extract of wheat straw containing the required amount of potassium was added to the nutrient solution. The Knop's solution was used in the control experiments. The experimental and control plants showed no significant differences in state or productivity of their photosynthetic apparatus. A small decrease in total productivity of the experimental plants was observed, which might result in some reduction of O{cyrillic}2 production in a BLSS. В© 2009 COSPAR.
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Gros J.-B., Lasseur C., Tikhomirov A.A., Manukovsky N.S., Ushakova S.A., Zolotukhin I.G., Gribovskaya I.V., Kovalev V.S.
Заглавие : Soil-like substrate for plant growing derived from inedible plant mass: Preparing, composition, fertility
Место публикации : Acta Horticulturae. - 2004. - Vol. 644. - С. 151-155
Ключевые слова (''Своб.индексиров.''): fertility--hydroponics--oyster mushroom--phytomass yield--wheat straw--worms
Аннотация: Fertility of soil-like substrate (SLS) made by successive conversion of wheat straw by oyster mushrooms and worms has been evaluated. Soil-like substrate of different degree of maturity has been tested. The most ready SLS provided the higher yields of wheat. It comprised 9.5% of humic acids, 4.9% of fulvic acids and 15.2% of nonhydrolyzable substances. At atmospheric concentration of carbon dioxide the soil-like substrate decreased its mass over the vegetation period by 14- 21%. The yield of wheat, beans and cucumbers grown on the soil-like substrate was compared to that on hydroponics.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Yu.A., Koyalev V.S., Gribovskaya I.V., Tirranen L.S., Zolotukhin I.G., Gros J.B., Lasseur Ch.
Заглавие : Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system
Место публикации : Acta Astronautica. - 2003. - Vol. 53, Is. 4-10. - С. 249-257. - ISSN 00945765 (ISSN) , DOI 10.1016/S0094-5765(03)00137-1
Ключевые слова (''Своб.индексиров.''): ecosystems--microorganisms--ph--photosynthesis--plants (botany)--synthesis (chemical)--waste utilization--biological life support systems (blls)--gas exchange--plant respiration--biomass--carbon dioxide--agaricales--article--biomass--bioremediation--growth, development and aging--hydroponics--incineration--metabolism--methodology--microbiology--microclimate--photosynthesis--plant physiology--radish--space flight--waste management--weightlessness--wheat--agaricales--biodegradation, environmental--biomass--carbon dioxide--ecological systems, closed--environmental microbiology--hydroponics--incineration--life support systems--photosynthesis--plant physiology--raphanus--space flight--triticum--waste management--weightlessness
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Califomian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. В© 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gros J.B., Lasseur Ch., Tikhomirov A.A., Manukovsky N.S., Kovalev V.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Karnachuk R.A., Dorofeev V.Yu.
Заглавие : Testing soil-like substrate for growing plants in bioregenerative life support systems
Место публикации : Advances in Space Research. - 2005. - Vol. 36, Is. 7. - С. 1312-1318. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.05.079
Ключевые слова (''Своб.индексиров.''): life support system--matter recycling--plants--soil-like substrate--biodiversity--correlation methods--growth kinetics--hormones--plants (botany)--recycling--soils--bioregeneration--life support system--matter recycling--soil-like substrate--space research
Аннотация: We studied soil-like substrate (SLS) as a potential candidate for plant cultivation in bioregenerative life support systems (BLSS). The SLS was obtained by successive conversion of wheat straw by oyster mushrooms and worms. Mature SLS contained 9.5% humic acids and 4.9% fulvic acids. First, it was shown that wheat, bean and cucumber yields as well as radish yields when cultivated on mature SLS were comparable to yields obtained on a neutral substrate (expanded clay aggregate) under hydroponics. Second, the possibility of increasing wheat and radish yields on the SLS was assessed at three levels of light intensity: 690, 920 and 1150 ?mol m-2 s-1 of photosynthetically active radiation (PAR). The highest wheat yield was obtained at 920 ?mol m-2 s-1, while radish yield increased steadily with increasing light intensity. Third, long-term SLS fertility was tested in a BLSS model with mineral and organic matter recycling. Eight cycles of wheat and 13 cycles of radish cultivation were carried out on the SLS in the experimental system. Correlation coefficients between SLS nitrogen content and total wheat biomass and grain yield were 0.92 and 0.97, respectively, and correlation coefficients between nitrogen content and total radish biomass and edible root yield were 0.88 and 0.87, respectively. Changes in hormone content (auxins, gibberellins, cytokinins and abscisic acid) in the SLS during matter recycling did not reduce plant productivity. Quantitative and species compositions of the SLS and irrigation water microflora were also investigated. Microbial community analysis of the SLS showed bacteria from Bacillus, Pseudomonas, Proteus, Nocardia, Mycobacterium, Arthrobacter and Enterobacter genera, and fungi from Trichoderma, Penicillium, Fusarium, Aspergillus, Mucor, Botrytis, and Cladosporium genera. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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