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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Barashkov V.A.
Заглавие : Characteristics of proteins synthesized by hydrogen-oxidizing microorganisms
Место публикации : Applied Biochemistry and Microbiology. - 2010. - Vol. 46, Is. 6. - С. 574-579. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683810060037
Ключевые слова (''Своб.индексиров.''): animalia--bacteria (microorganisms)--cupriavidus necator--pseudomonas carboxydohydrogena
Аннотация: The study was conducted to determine the biological value of proteins synthesized by hydrogen-oxidizing microorganisms-the hydrogen bacteria Alcaligenes eutrophus Z1 and Ralstonia eutropha B5786 and the CO-resistant strain of carboxydobacterium Seliberia carboxydohydrogena Z1062. Based on a number of significant parameters characterizing the biological value of a product, the proteins of hydrogen-oxidizing microorganisms have been found to occupy an intermediate position between traditional animal and plant proteins. The high total protein in biomass of these microorganisms, their complete amino acid content, and availability to proteolytic enzymes allow for us to consider these microorganisms as potential protein producers. В© 2010 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Gubanenko G.A., Demirchieva S.M., Kalachova G.S.
Заглавие : Effect of boiling and frying on the content of essential polyunsaturated fatty acids in muscle tissue of four fish species
Место публикации : Food Chemistry. - 2007. - Vol. 101, Is. 4. - С. 1694-1700. - ISSN 03088146 (ISSN) , DOI 10.1016/j.foodchem.2006.04.029
Ключевые слова (''Своб.индексиров.''): cod--essential polyunsaturated fatty acids--herring--sole--trout--docosahexaenoic acid--icosapentaenoic acid--polyunsaturated fatty acid--article--atlantic cod--atlantic herring--boiling point--brown trout--controlled study--cooking--fish--food processing--frying--lepidopsetta bilineata--muscle tissue--nonhuman--norway--raw meat--russian federation--sample--clupea pallasi--clupeidae--gadus ogac--lepidopsetta bilineata--martes pennanti--paraplagusia bilineata--salmo trutta--salmonidae
Аннотация: Frozen samples of common fish species, sea trout (Salmo trutta), from Norway and Siberia, herring (Clupea harengus pallasi), rock sole (Lepidopsetta bilineata) and cod (Gadus morhua maris-albi), collected from a wholesale market in Krasnoyarsk city (Siberia, Russia) were analyzed. Special attention was paid to long-chain essential polyunsaturated fatty acids: eicosapentaenoic, 20:5?3 (EPA) and docosahexaenoic, 22:6?3 (DHA). Heat-treatment (cooking and frying) did not in general significantly decrease the contents of EPA and DHA compared to raw fish species, except for a modest reduction in Norwegian trout during frying. Boiled trout appeared to be a more valuable fish dish for obtaining the officially recommended appropriate daily intake of EPA + DHA for humans. Herring and sole had intermediate values, while boiled cod had a comparatively low value. В© 2006 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Frank L.A., Visser AJWG, van Berkel WJH, Vysotski E.S.
Заглавие : Bioluminescent and spectroscopic properties of His-Trp-Tyr triad mutants of obelin and aequorin
Колич.характеристики :9 с
Место публикации : Photochem. Photobiol. Sci.: ROYAL SOC CHEMISTRY, 2013. - Vol. 12, Is. 6. - С. 1016-1024. - ISSN 1474-905X, DOI 10.1039/c3pp00002h
Примечания : Cited References: 46. - The work was supported by RFBR grant 12-04-00131, by the Programs of the Government of Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.0058), "Molecular and Cellular Biology" of RAS, President of Russian Federation "Leading science school" (grant 1044.2012.2). E.V.E. was supported by Wageningen University Sandwich PhD-Fellowship Program.
Предметные рубрики: CA2+-REGULATED PHOTOPROTEINS
CA2+-BINDING PHOTOPROTEIN
SEQUENCE-ANALYSIS
CRYSTAL-STRUCTURE
VIOLET BIOLUMINESCENCE
ANGSTROM RESOLUTION
MNEMIOPSIS-LEIDYI
LIGHT-EMISSION
W92F OBELIN
CLONING
Аннотация: Ca2+-regulated photoproteins are responsible for the bioluminescence of a variety of marine organisms, mostly coelenterates. The photoproteins consist of a single polypeptide chain to which an imidazopyrazinone derivative (2-hydroperoxycoelenterazine) is tightly bound. According to photoprotein spatial structures the side chains of His175, Trp179, and Tyr190 in obelin and His169, Trp173, Tyr184 in aequorin are at distances that allow hydrogen bonding with the peroxide and carbonyl groups of the 2-hydroperoxycoelenterazine ligand. We replaced these amino acids in both photoproteins by residues with different hydrogen bond donor-acceptor capacity. All mutants exhibited luciferase-like bioluminescence activity, hardly present in the wild-type photoproteins, and showed low or no photoprotein activity, except for aeqH169Q (24% of wild-type activity), obeW179Y (23%), obeW179F (67%), obeY190F (14%), and aeqY184F (22%). The results clearly support the supposition made from photoprotein spatial structures that the hydrogen bond network formed by His-Trp-Tyr triad participates in stabilizing the 2-hydroperoxy adduct of coelenterazine. These residues are also essential for the positioning of the 2-hydroperoxycoelenterazine intermediate, light emitting reaction, and for the formation of active photoprotein. In addition, we demonstrate that although the positions of His-Trp-Tyr residues in aequorin and obelin spatial structures are almost identical the substitution effects might be noticeably different.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Malikova N.P., Visser N.V., van Hoek A..., Skakun V.V., Vysotski E.S., Lee J..., Visser AJWG
Заглавие : Green-Fluorescent Protein from the Bioluminescent Jellyfish Clytia gregaria Is an Obligate Dimer and Does Not Form a Stable Complex with the Ca2+-Discharged Photoprotein Clytin
Колич.характеристики :10 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 2011. - Vol. 50, Is. 20. - С. 4232-4241. - ISSN 0006-2960, DOI 10.1021/bi101671p
Примечания : Cited References: 50. - This work was supported by NATO Collaborative Linkage Grant 979229, Grants SB RAS No. 2 and RFBR 08-04-92209, 09-04-12022, and 09-04-00172, the MCB program of the Russian Academy of Sciences, and Bayer AG.
Предметные рубрики: VIBRIO-FISCHERI Y1
ENERGY-TRANSFER
CORRELATION SPECTROSCOPY
BACTERIAL LUCIFERASE
REFRACTIVE-INDEX
PHOTOBACTERIUM-LEIOGNATHI
POLARIZED FLUORESCENCE
EXCITATION TRANSFER
RECOMBINANT OBELIN
LUMAZINE PROTEIN
Аннотация: Green-fluorescent protein (GFP) is the origin of the green bioluminescence color exhibited by several marine hydrozoans and anthozoans. The mechanism is believed to be Forster resonance energy transfer (FRET) within a luciferase GFP or photoprotein-GFP complex. As the effect is found in vitro at micromolar concentrations, for FRET to occur this complex must have an affinity in the micromolar range. We present here a fluorescence dynamics investigation of the recombinant bioluminescence proteins from the jellyfish Clytia gregaria, the photoprotein clytin in its Ca2+-discharged form that is highly fluorescent (lambda(max) = 506 nm) and its GFP (cgreGFP; lambda(max) = 500 nm). Ca2+-discharged clytin shows a predominant fluorescence lifetime of 5.7 ns, which is assigned to the final emitting state of the bioluminescence reaction product, coelenteramide anion, and a fluorescence anisotropy decay or rotational correlation time of 12 ns (20 degrees C), consistent with tight binding and rotation with the whole protein. A 34 ns correlation time combined with a translational diffusion constant and molecular brightness from fluorescence fluctuation spectroscopy all confirm that cgreGFP is an obligate dimer down to nanomolar concentrations. Within the dimer, the two chromophores have a coupled excited-state transition yielding fluorescence depolarization via FRET with a transfer correlation time of 0.5 ns. The 34 ns time of cgreGFP showed no change upon addition of a 1000-fold excess of Ca2+-discharged clytin, indicating no stable complexation below 0.2 mM. It is proposed that any bioluminescence FRET complex with micromolar affinity must be one formed transiently by the cgreGFP dimer with a short-lived (millisecond) intermediate in the clytin reaction pathway.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stepanyuk G.A., Liu Z.J., Markova S.S., Frank L.A., Lee J..., Vysotski E.S., Wang B.C.
Заглавие : Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 angstrom: Why it is not a calcium-regulated photoprotein
Колич.характеристики :6 с
Место публикации : Photochem. Photobiol. Sci.: ROYAL SOC CHEMISTRY, 2008. - Vol. 7, Is. 4. - С. 442-447. - ISSN 1474-905X, DOI 10.1039/b716535h
Примечания : Cited References: 49
Предметные рубрики: HYDROID OBELIA-GENICULATA
AMINO-ACID-SEQUENCE
CA2+-REGULATED PHOTOPROTEINS
RENIFORMIS LUCIFERASE
ENERGY-TRANSFER
CDNA CLONING
BIOLUMINESCENCE
AEQUORIN
PURIFICATION
EXPRESSION
Аннотация: Bioluminescence in the sea pansy Renilla involves two distinct proteins, a Ca2+-triggered coelenterazine-binding protein (CBP), and Renilla luciferase. CBP contains one tightly bound coelenterazine molecule, which becomes available for reaction with luciferase and O-2 only subsequent to Ca2+ binding. CBP belongs to the EF-hand superfamily of Ca2+-binding proteins and contains three "EF-hand" Ca2+-binding sites. The overall spatial structure of recombinant selenomethionine-labeled CBP determined at 1.7 angstrom, is found to approximate the protein scaffold characteristic of the class of Ca2+-regulated photoproteins. Photoproteins however, catalyze molecular oxygen addition to coelenterazine producing a 2-hydroperoxycoelenterazine intermediate, which is stabilized within the binding cavity in the absence of Ca2+. Addition of Ca2+ triggers the bioluminescence reaction. However in CBP this first step of oxygen addition is not allowed. The different amino acid environments and hydrogen bond interactions within the binding cavity are proposed to account for the different properties of the two classes of proteins.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Frank L.A., Vysotski E.S.
Заглавие : THE MAIN FUNCTION OF HIS175, TRP179, AND TYR190 RESIDUES OF THE OBELIN BINDING SITE IS TO STABILIZE THE HYDROPEROXYCOELENTERAZINE INTERMEDIATE
Колич.характеристики :4 с
Место публикации : BIOLUMINESCENCE AND CHEMILUMINESCENCE: CHEMISTRY, BIOLOGY AND APPLICATIONS: WORLD SCIENTIFIC PUBL CO PTE LTD, 2007. - 14th International Symposium on Bioluminescence and Chemiluminescence (OCT 15-19, 2006, San Diego, CA). - С. 7-10. - ISBN 978-981-270-816-8, DOI 10.1142/9789812770196_0002
Примечания : Cited References: 7
Предметные рубрики: PHOTOPROTEIN OBELIN
BIOLUMINESCENCE
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Frank L.A., Lee J..., Vysotski E.S.
Заглавие : The main function of His175, Trp179, and Tyr190 residues of the obelin-binding site is to stabilize the hydroperoxycoelenterazine intermediate
Колич.характеристики :2 с
Место публикации : Luminescence: WILEY-BLACKWELL, 2006. - Vol. 21, Is. 5. - С. 275-276. - ISSN 1522-7235
Примечания : Cited References: 0
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Dubinnyi M.A., Rodionova N.S., Nadezhdin K.D., Marques S.M., Esteves Da Silva J.C.G., Shimomura O., Yampolsky I.V.
Заглавие : AsLn2, a luciferin-related modified tripeptide from the bioluminescent earthworm Fridericia heliota
Место публикации : Tetrahedron Letters. - 2014. - Vol. 55, Is. 2. - С. 463-465. - ISSN 00404039 (ISSN) , DOI 10.1016/j.tetlet.2013.11.061
Ключевые слова (''Своб.индексиров.''): bioluminescence--fridericia heliota--luciferin--modified peptide
Аннотация: AsLn2, an unusual modified peptide, was isolated from the bioluminescent earthworm Fridericia heliota (Enchytraeidae). Its structure, elucidated by NMR and mass spectrometry, includes residues of tyrosine, CompX (a novel tyrosine modification product, reported in the accompanying paper), and N(omega)-acylated lysine. Chromatography, UV, and 1H NMR data imply a close structural similarity of AsLn2 with F. heliota luciferin. AsLn2 appears to be an intermediate or by-product in F. heliota luciferin biosynthesis. © 2013 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Ketelaars M..., Gibson B.G., Lee J...
Заглавие : Interaction of Photobacterium leiognathi and Vibrio fischeri Y1 luciferases with fluorescent (Antenna) proteins: Bioluminescence effects of the aliphatic additive
Колич.характеристики :8 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 1996. - Vol. 35, Is. 37. - С. 12086-12093. - ISSN 0006-2960, DOI 10.1021/bi9608931
Примечания : Cited References: 41
Предметные рубрики: BACTERIAL LUCIFERASE
LUMAZINE PROTEIN
FLAVIN INTERMEDIATE
ANGSTROM RESOLUTION
RIBOFLAVIN PROTEIN
PURIFICATION
MECHANISM
EMISSION
ALDEHYDE
INHIBITION
Аннотация: The kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (LumP) from Photobacterium or the yellow fluorescence proteins (YFP) having FMN or Rf bound, from Vibrio fischeri strain Y1, Depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. These effects call be simply explained on the basis of the same protein-protein complex model that accounts for the bioluminescence spectral shifts induced by these fluorescent proteins. In such a complex, when the fluorophore evidently is in proximity to the luciferase active site, it is expected that the on-off rate of certain aliphatic components of the reaction should be altered with a consequent shift in the equilibria among the luciferase intermediates, as recently elaborated in a kinetic scheme, These aliphatic components are the bioluminescence reaction substrate, tetradecanal or other long-chain aldehyde, its carboxylic acid product, or dodecanol used as a stabilizer of the luciferase peroxyflavin. No evidence can be found or the protein-protein interaction in the absence of the aliphatic component.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J...
Заглавие : Direct measurement of excitation transfer in the protein complex of bacterial luciferase hydroxyflavin and the associated yellow fluorescence proteins from Vibrio fischeri Y1
Колич.характеристики :6 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 1996. - Vol. 35, Is. 25. - С. 8413-8418. - ISSN 0006-2960, DOI 10.1021/bi952691v
Примечания : Cited References: 24
Предметные рубрики: LUMAZINE PROTEIN
LUMINOUS BACTERIUM
STRAIN Y-1
BIOLUMINESCENCE
EMISSION
PURIFICATION
TRANSIENT
LIGHT
Аннотация: Time-resolved fluorescence was used to directly measure the energy transfer rate constant in the protein-protein complex involved in the yellow bioluminescence of Vibrio fischeri, strain Y1. In this reaction the putative donor is the fluorescent transient intermediate, luciferase hydroxyflavin, which exhibits a major fluorescence lifetime of the bound flavin of 10 ns. On addition of the acceptor, the V. fischeri yellow fluorescence protein containing either FMN or riboflavin as ligand, a rapid decay time, 0.25 ns, becomes predominant. The same results are observed using rec-luciferase from Photobacterium leiognathi to produce the donor. Because of favorable spectral separation in this system, this rapid decay rate of 4 ns(-1), can be directly equated to the energy transfer rate. This rate is ten times higher than the rate previously observed in the Photobacterium luciferase hydroxyflavin-lumazine protein, donor-acceptor system, derived from emission anisotropy measurements. This ten-times ratio is close to the ratio of spectral overlaps of the donor fluorescence with the acceptor absorption, between these two systems, so it is concluded that the topology of the protein complexes in both cases, must be very similar. Energy transfer is also monitored by the loss of steady-state fluorescence intensity at 460 nm of the donor, on addition of the acceptor protein. A fluorescence titration indicates that luciferase hydroxyflavin and the yellow protein complex with a 1:1 stoichiometry with a K-d of 0.7 mu M (0 degrees C). These parameters account for the bioluminescence spectral shifting effects observed in these reactions.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tyulkova N.A., Sandalova T.P.
Заглавие : Comparative study of temperature effects on bacterial luciferases
Колич.характеристики :10 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1996. - Vol. 61, Is. 2. - P205-214. - ISSN 0006-2979
Примечания : Cited References: 23
Предметные рубрики: BIOLUMINESCENCE
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--temperature--activation energy
Аннотация: Effects of temperature on bioluminescent patterns of luciferases from luminescent bacteria Vibrio harveyi, Vibrio fischeri, Photobacterium leiognathi, and Photobacterium phosphoreum were studied. The highest luminescence level was observed at 15-25 degrees C for the luciferase from P. phosphoreum, at 20-30 degrees C for the V. fischeri and P. leiognathi enzymes, and at 30-37 degrees C for the enzyme from V. harveyi. All the luciferases were significantly stabilized at increased salt concentrations, at low pH values, or in the presence of dithiothreitol (DTT) and EDTA. The addition of DTT and EDTA affected the reversible stage of enzyme inactivation, while salts reduced the rate of the irreversible stage. A peak corresponding to aggregated protein was detected by gel chromatography of irreversibly inactivated luciferase. Activation energies were calculated for each luciferase in bioluminescent reactions with decanal, dodecanal, tetradecanal, and without aldehydes. The activation energy of the reaction with tetradecanal was much lower than those with the other aldehydes. The temperature dependence of the lifetime of the long-lived reaction intermediate showed that in the 10-30 degrees C interval all the luciferases, except for the enzyme from V. harveyi, have only one active form.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kirsanova E.N., Sadovsky M.G.
Заглавие : Entropy approach in the analysis of anisotropy of digital images
Колич.характеристики :12 с
Место публикации : Open Syst. Inf. Dyn.: KLUWER ACADEMIC PUBL, 2002. - Vol. 9, Is. 3. - P239-250. - ISSN 1230-1612, DOI 10.1023/A:1019704411382
Примечания : Cited References: 15
Аннотация: Anisotropy is assumed to be the difference of a plane object observed in different dimensions. For digital images, anisotropy is determined in two ways. The first one is based on the comparison of mosaics bearing rectangular smalts developed in different (perpendicular, to be exact) directions. The comparison is provided through an intermediate mosaic called palette, that is the mosaic with the frequency of smalts equal to arithmetic mean of the frequency of smalts of compared mosaics. The latter is based on the calculation of the information capacity of the mosaics developed in different directions. The information capacity is the specific entropy of real mosaic calculated against the reconstructed one bearing the most probable expansions of smaller smalts. The problem of test object is discussed.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N. N., Rudchenko A. E., Gladyshev M. I.
Заглавие : Effect of season and trophic level on fatty acid composition and content of four commercial fish species from Krasnoyarsk Reservoir (Siberia, Russia)
Место публикации : Fish. Res.: Elsevier B.V., 2017. - Vol. 187. - С. 178-187. - ISSN 01657836 (ISSN) , DOI 10.1016/j.fishres.2016.11.016
Ключевые слова (''Своб.индексиров.''): fatty acids--piscivorous and omnivorous fish--season--stable isotopes--trophic level
Аннотация: Two groups of factors, phylogenetic and ecological, are presently regarded as controlling fatty acid composition of fish, including essential eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Environmental effects, e.g., trophic position, temperature and/or seasonality, were previously studied using sums of fatty acids or only their level data. We tested the hypothesis that differences in trophic levels of piscivorous (pike and perch) and omnivorous (roach and bream) fish from a mesotrophic reservoir allow discriminating levels and contents of individual fatty acids, especially EPA and DHA. The more established measurements, i.e., stomach contents and carbon and nitrogen stable isotopes in fish muscles, were also carried out to provide linkages between the different ecological tracers, fatty acids versus stable isotopes, and matching the methods for long-term food sources (fatty acids and stable isotopes) and recent foraging (stomach content analysis). We also studied a putative influence of seasonality. Similar to other studies, there were seasonal changes in fatty acid composition and contents of two fish, perch and roach, due to direct and indirect effects of water temperature. Meanwhile, the piscivorous and omnivorous species captured in the same month, were explicitly differentiated on a base of stable isotopes and fatty acids. Significantly higher percentages and contents of DHA in piscivorous fish, perch and pike, relatively to those in roach and bream, likely indicated a higher trophic transfer efficiency for this essential fatty acid. All the fishes have commercial importance for regional fishery and are harvested from the studied reservoir for human nutrition. Regarding content of EPA + DHA (mg g?1 fish) as the indicator of nutritive value for humans, pike had the highest nutritive value, roach and perch had intermediate overlapped values, and bream was of the least benefit. © 2016 Elsevier B.V.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kaskova, Zinaida M., Dorr, Felipe A., Petushkov, Valentin N., Purtov, Konstantin V., Tsarkova, Aleksandra S., Rodionova, Natalja S., Mineev, Konstantin S., Guglya, Elena B., Kotlobay, Alexey, Baleeva, Nadezhda S., Baranov, Mikhail S., Arseniev, Alexander S., Gitelson, Josef I., Lukyanov, Sergey, Suzuki, Yoshiki, Kanie, Shusei, Pinto, Ernani, Di Mascio, Paolo, Waldenmaier, Hans E., Pereira, Tatiana A., Carvalho, Rodrigo P., Oliveira, Anderson G., Oba, Yuichi, Bastos, Erick L., Stevani, Cassius V., Yampolsky, Ilia V.
Заглавие : Mechanism and color modulation of fungal bioluminescence
Колич.характеристики :8 с
Коллективы : Sao Paulo Research Foundation [FAPESP] [10/11578-5, 13/16885-1, 14/14866-2, 13/07914-8, 2012/12663-1]; CEPID Redoxoma [2013/07937-8]; National Council for Scientific and Technological Development (CNPq) [301307/2013-0]; NAP Redoxoma (PRPUSP) [2011.1.9352.1.8]; Japan Society for the Promotion of Science [16K07715]; Chubu University [AII28II M01]; Russian Science Foundation [16-14-00052]
Место публикации : Sci. Adv.: AMER ASSOC ADVANCEMENT SCIENCE, 2017. - Vol. 3, Is. 4. - Ст.e1602847. - ISSN 2375-2548, DOI 10.1126/sciadv.1602847
Примечания : Cited References:40. - This work was supported by the Sao Paulo Research Foundation [FAPESP grants 10/11578-5 (to A.G.O.), 13/16885-1 (to C.V.S.), 14/14866-2 (to E.L.B.), 13/07914-8 (to E.P. and F.A.D.), and 2012/12663-1 (to P.D.M.) and CEPID Redoxoma 2013/07937-8 (to P.D.M.)], the National Council for Scientific and Technological Development (CNPq) [301307/2013-0 (to P.D.M.)], NAP Redoxoma (PRPUSP) [2011.1.9352.1.8. (to P.D.M.)], the Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) [grant no. 16K07715 (to Y.O.)], Chubu University [grant AII28II M01 (to Y.O.)], and the Russian Science Foundation (grant 16-14-00052 to all Russian authors).
Предметные рубрики: SINGLET MOLECULAR-OXYGEN
QUANTUM YIELDS
CHEMILUMINESCENCE
Аннотация: Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nemtseva, Elena V., Lashchuk, Olesya O., Gerasimova, Marina A., Melnik, Tatiana N., Nagibina, Galina S., Melnik, Bogdan S.
Заглавие : Fluorescence lifetime components reveal kinetic intermediate states upon equilibrium denaturation of carbonic anhydrase II
Колич.характеристики :9 с
Коллективы : Ministry for Science and Education of the Russian Federation [6.7734.2017/BCH]; Russian Science Foundation [N14-24-00157]
Место публикации : Methods Appl. Fluoresc.: IOP PUBLISHING LTD, 2018. - Vol. 6, Is. 1. - Ст.015006. - ISSN 2050-6120, DOI 10.1088/2050-6120/aa994a
Примечания : Cited References:28. - The study of time-resolved protein fluorescence was supported by the Ministry for Science and Education of the Russian Federation (project 6.7734.2017/BCH). Kinetic and genetic engineering studies of carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation.
Предметные рубрики: PROTEIN FLUORESCENCE
TRYPTOPHAN PROTEINS
RESIDUES
STABILITY
Ключевые слова (''Своб.индексиров.''): time-resolved fluorescence spectroscopy--carbonic anhydrase ii--protein--intermediate states--comparison of kinetic and equilibrium experiments--protein fluorescence lifetime
Аннотация: In most cases, intermediate states of multistage folding proteins are not 'visible' under equilibrium conditions but are revealed in kinetic experiments. Time-resolved fluorescence spectroscopy was used in equilibrium denaturation studies. The technique allows for detecting changes in the conformation and environment of tryptophan residues in different structural elements of carbonic anhydrase II which in its turn has made it possible to study the intermediate states of carbonic anhydrase II under equilibrium conditions. The results of equilibrium and kinetic experiments using wild-type bovine carbonic anhydrase II and its mutant form with the substitution of leucine for alanine at position 139 (L139A) were compared. The obtained lifetime components of intrinsic tryptophan fluorescence allowed for revealing that, the same as in kinetic experiments, under equilibrium conditions the unfolding of carbonic anhydrase II ensues through formation of intermediate states.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova N. G., Pyatina S. А., Nikolaeva E. D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov D. B., Sakhnov E. V., Shishatskaya E. I.
Заглавие : Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients
Место публикации : Toxicol. Rep.: Elsevier Inc., 2019. - Vol. 6. - С. 74-90. - ISSN 22147500 (ISSN) , DOI 10.1016/j.toxrep.2018.11.009
Ключевые слова (''Своб.индексиров.''): atherosclerosis--cell morphology--intravascular stenting--macrophages--monocytes--polyhydroxyalkanoates
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients. © 2018 The Authors
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gulnov D. V., Nemtseva E. V., Gerasimova M. A., Kratasyuk V. A.
Заглавие : Structural transitions of photobacterium leiognathi luciferase determined by various optical techniques under urea-induced equilibrium denaturation
Место публикации : Tsitologiya: Sankt Peterburg, 2018. - Vol. 60, Is. 10. - С. 847-850. - ISSN 00413771 (ISSN) , DOI 10.7868/S0041377118100181
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--circular dichroism--denaturation--protein fluorescence lifetime--protein intermediate states
Аннотация: The study was aimed to identification of conformational transitions of Photobacterium leiognathi luciferase during equilibrium denaturation with urea using several optical techniques, including circular dichroism, stationary and time-resolved fluorescence. Gravity center and intensity ratio I 325 /I 390 for the fluorescence spectra, molar ellipticity at 222 nm and fluorescence lifetimes of the protein were analyzed. Investigated parameters revealed two possible transitions for P. leiognathi luciferase with the midpoints at 0.5—1.1 and 3.5—4.2 M of urea. Changes in the values of two lifetime components, characterizing the luciferase fluorescence reflect both transitions, while steady-state fluorescence parameters (gravity center of spectrum and I 325 /I 390 ratio) reveal only the second one. Far-UV circular dichroism spectra displayed transitions at 4.2 M of urea for P. leiognathi luciferase. Conformational transitions characteristics of P. leiognathi luciferase and previously studied Vibrio harveyi luciferase (Inlow et al., 2002) were compared. Since, according to the published data for V. harveyi, midpoint of the second conformational transition is at about 2.5 M of urea, the results indicate more stable secondary structure for the P. leiognathi luciferase under study. The possible reasons for observed differences in fluorescent characteristics of two types of luciferases during denaturation can be connected to the microenvironment variation of the tryptophan residues in their tertiary structure, namely in position 131 and 277 in a-subunit. © 2018 Sankt Peterburg. All rights reserved.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Melgunov M.
Заглавие : Radioactive particles in the Yenisei River floodplain (Russia): Characterization, leaching and potential effects in the environment
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2019. - Vol. 208-209. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2019.105991
Аннотация: The operation of the Mining-and-Chemical Combine (MCC), the largest producer of weapons-grade plutonium in Russia, has resulted in radioactive contamination of the Yenisei River floodplain. Investigations carried out in Novosibirsk and Krasnoyarsk institutes have shown that the floodplain of the Yenisei downstream of the MCC is contaminated by radioactive particles (RP) of various types and activities. Analytical characterization of the RP showed that most of them were fuel particles, which were carried into the Yenisei after incidents at the MCC reactors. The plutonium and caesium isotope ratios (238Pu/239,240Pu; 137Cs/134Cs) vary substantially between the particles, indicating different source terms and time intervals when the RP were formed. In addition to fuel RP, there were particles that contained activation radionuclides. The experiment on dissolution of RP using the model solution (the simulated stomach fluid) showed different cumulative extractions of radionuclides from the particles: 60Co and 137Cs extractions were the lowest, the extracted fractions of europium and americium isotopes were the largest, and plutonium occupied an intermediate position. High concentrations of radionuclides in RP are sources of exposure of organisms in aquatic and terrestrial ecosystems to low radiation doses. The plant bioassays of the effects of ?-radiation from RP showed the effect of low doses of ?-radiation on growth parameters of aquatic plant Elodea canadensis growing in the Yenisei River floodplain. The presence of RP from different sources in the Yenisei River floodplain makes this region a unique site for studying environmental effects of the particles. © 2019 Elsevier Ltd
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lisitsa A. E., Sukovatyi L. A., Kratasyuk V. A., Nemtseva E. V.
Заглавие : Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction
Место публикации : Doklad. Biochem. Biophys.: Pleiades Publishing, 2020. - Vol. 492, Is. 1. - С. 162-165. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672920020106
Аннотация: Abstract: The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1–1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82–0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products. © 2020, Pleiades Publishing, Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lisitsa A. E., Sukovatyi L. A., Kratasyuk V. A., Nemtseva E. V.
Заглавие : Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction
Колич.характеристики :4 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [6.7734.2017, 01201351504]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 492, Is. 1. - С. 162-165. - ISSN 1607-6729, DOI 10.1134/S1607672920020106. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15. - This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project nos. 6.7734.2017 and 01201351504).
Предметные рубрики: LUCIFERASE
Аннотация: The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1-1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82-0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products.
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