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А.с. 1035962 СССР, МКИ C 12 N 9/02.

Способ получения препарата бактериальной люциферазы [Текст] / В. В. Межевикин [и др.] ; Ин-т биофиз. СО АН СССР. - № 3402201/28-13 ; Заявл. 21.01.19821983. -

ГРНТИ

34.27.51

РУБ 343.27.51
Рубрики:
ЛЮЦЕФЕРАЗА
   ПОЛУЧЕНИЕ
   СПОСОБ
   PHOTOBACTERIUM LEIOGNATHI
   БАКТЕРИИ
   ENZYME ISOLATION
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Межевикин, В.В.; Высоцкий, Е.С.; Заворуев, В.В.; Родичева, Э.К.; Ин-т биофиз. СО АН СССР
Свободных экз. нет
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The use of glowing wood as a source of luminescent culture of fungus mycelium [Text] / A. P. Puzyr, S. E. Medvedeva, V. S. Bondar // Mycosphere. - 2016. - Vol. 7, Is. 1. - P1-17, DOI 10.5943/mycosphere/7/1/1. - Cited References:22. - The authors are grateful to Prof. A. Frank, Director of North Borneo Biostation, for the opportunity to carry out studies of glowing wood; to Nadezhda N. Kudashova, a senior researcher at the Institute of Biology and Biophysics at the Tomsk University, for identifying the species of nonluminous fungi. This study was supported by grant no. 11.G34.31.0058 (RF Government) and Projects no. 71 (SB RAS). . - ISSN 2077-7000

РУБ Mycology
Рубрики:
BIOLUMINESCENCE CHARACTERISTICS
NEONOTHOPANUS-NAMBI
LIGHT-EMISSION
Кл.слова (ненормированные):
Bioluminescence -- culture of luminous mycelia -- kinetics of luminescent -- reaction -- light emitting wood -- luminous fungus
Аннотация: In studies of fungal bioluminescence, not only fruiting bodies and spores of the fungus, but also samples of luminescent wood, leaf litter or soil may need to be used to derive pure mycelial culture. This study describes an approach to isolating the culture of luminescent fungal mycelium from samples of light-emitting wood found on Borneo Island in November-December 2013. A GelDoc XR Imaging System (Bio-Rad Laboratories, Inc., U.S.) was used for the first time to monitor luminescence and select luminous samples. This study shows that for successful isolation of the culture of luminescent mycelium out of the luminescent wood found in the forest, it is imperative to keep the samples moist (mycelium alive until there is water), while immediate and aseptic delivery of the samples to the laboratory is not a crucial condition (inner layers of wood is "sterile"). Investigation of the growth features of the isolated mycelium in various growing conditions revealed some peculiar properties of its luminescence in comparison with the known luminescent cultures of basidiomycetes. When grown on solid nutrient media, mycelium exhibits low growth rates, long-lasting luminescence (140 days or longer), and emergence and disappearance of local zones with high levels of light emission. Mycelium produced in submerged culture does not emit light, and this effect must be caused by the absence or a very low level of the luminescent reaction substrate in the biomass. The luminescence system isolated from mycelial biomass did not induce luminescent reaction in vitro upon the addition of NADPH (recording intensity is 60 100 URL/sec). We found that enzymes of the luminescence systems isolated from mycelium pellets retained their activity and catalyzed luminescent reaction when a hot extract of the luminous fungus Armillaria sp. (IBSO 2360) was added (near 1900 URL/sec). The same effect was obtained after addition of hot extracts from the fruiting bodies of nonluminous higher fungi Pholiota squarrosa, Cortinarius sp., Hypholoma capnoides and Chroogomphus rutilus (near 3500 URL/sec). The pure culture of luminescent mycelium has been registered in the Culture Collection of IBP SB RAS as IBSO 2371; now it can be used for various in vivo and in vitro studies, including identification of the fungus.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Puzyr, A. P.; Medvedeva, S. E.; Bondar, V. S.; RF Government [11.G34.31.0058]; SB RAS [71]

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Study of the luminescence system of the soil enchytraeid Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae) / V. N. Petushkov, N. S. Rodionova, V. S. Bondar' // Doklady Biochemistry and Biophysics. - 2003. - Vol. 391, Is. 1-6. - P204-207, DOI 10.1023/A:1025105323648 . - ISSN 1607-6729
Кл.слова (ненормированные):
adsorption chromatography -- animal cell -- annelid worm -- article -- luminescence -- nonhuman -- purification -- animal -- drug antagonism -- isolation and purification -- metabolism -- physiology -- soil -- Animalia -- Annelida -- Clitellata -- Enchytraeidae -- edetic acid -- luciferase -- magnesium -- Animals -- Edetic Acid -- Luciferases -- Luminescent Measurements -- Magnesium -- Oligochaeta -- Soil

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Rodionova, N.S.; Bondar', V.S.

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Structure of hydrocarbons synthesized by the alga Botryococcus isolated from Lake Shira. / N. O. Zhila [et al.] // Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2001. - Vol. 378. - P265-269 . - ISSN 0012-4966
Кл.слова (ненормированные):
fatty acid -- hydrocarbon -- sea water -- article -- chemical structure -- chemistry -- classification -- green alga -- isolation and purification -- metabolism -- microbiology -- Russian Federation -- species difference -- Algae, Green -- Fatty Acids -- Hydrocarbons -- Molecular Structure -- Seawater -- Siberia -- Species Specificity

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhila, N.O.; Kalacheva, G.S.; Volova, T.G.; Degermendzhi, A.G.

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Species composition of winter bacterioplankton in blooming and nonblooming reservoirs as determined by 16S rRNA sequences / M. Yu. Trusova, M. I. Gladyshev // Doklady Biological Sciences. - 2005. - Vol. 405, Is. 1-6. - P443-445, DOI 10.1007/s10630-005-0160-4 . - ISSN 0012-4966
Кл.слова (ненормированные):
fresh water -- RNA 16S -- article -- bacterium -- classification -- genetic variability -- genetics -- isolation and purification -- microbiology -- molecular genetics -- phylogeny -- plankton -- Russian Federation -- season -- species difference -- Bacteria -- Fresh Water -- Molecular Sequence Data -- Phylogeny -- Plankton -- RNA, Ribosomal, 16S -- Seasons -- Siberia -- Species Specificity -- Variation (Genetics) -- Water Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Yu.; Gladyshev, M.I.

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Redquorinxs mutants with enhanced calcium sensitivity and bioluminescence output efficiently report cellular and neuronal network activities / A. Bakayan, S. Picaud, N. P. Malikova [et al.] // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 21. - Ст. 7846. - P1-22, DOI 10.3390/ijms21217846 . - ISSN 1661-6596
Кл.слова (ненормированные):
Aequorin -- Bioluminescence -- BRET -- Calcium sensor -- GPCR assay -- Mutagenesis -- Neuronal network imaging
Аннотация: Considerable efforts have been focused on shifting the wavelength of aequorin Ca2+? dependent blue bioluminescence through fusion with fluorescent proteins. This approach has notably yielded the widely used GFP?aequorin (GA) Ca2+ sensor emitting green light, and tdTomato-aequorin (Redquorin), whose bioluminescence is completely shifted to red, but whose Ca2+ sensitivity is low. In the present study, the screening of aequorin mutants generated at twenty?four amino acid positions in and around EF?hand Ca2+?binding domains resulted in the isolation of six aequorin single or double mutants (AequorinXS) in EF2, EF3, and C?terminal tail, which exhibited markedly higher Ca2+ sensitivity than wild?type aequorin in vitro. The corresponding Redquorin mutants all showed higher Ca2+ sensitivity than wild?type Redquorin, and four of them (RedquorinXS) matched the Ca2+ sensitivity of GA in vitro. RedquorinXS mutants exhibited unaltered thermostability and peak emission wavelengths. Upon stable expression in mammalian cell line, all RedquorinXS mutants reported the activation of the P2Y2 receptor by ATP with higher sensitivity and assay robustness than wt?Redquorin, and one, RedquorinXS?Q159T, outperformed GA. Finally, wide?field bioluminescence imaging in mouse neocortical slices showed that RedquorinXS?Q159T and GA similarly reported neuronal network activities elicited by the removal of extracellular Mg2+. Our results indicate that RedquorinXS?Q159T is a red light?emitting Ca2+ sensor suitable for the monitoring of intracellular signaling in a variety of applications in cells and tissues, and is a promising candidate for the transcranial monitoring of brain activities in living mice. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Institut de Neurobiologie Alfred Fessard, UPR 3294, Centre National de la Recherche Scientifique (CNRS), Avenue de la Terrasse, Gif?sur?Yvette, 91198, France
BioEmergences Unit, CNRS USR 3695, Universite Paris?Saclay, Avenue de la Terrasse, Gif?sur?Yvette, 91198, France
Neuroscience Paris Seine ? Institut de Biologie Paris Seine (NPS ? IBPS), CNRS, UMR8246, INSERM U1130, Sorbonne Universite UM119, Paris, 75005, France
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Bakayan, A.; Picaud, S.; Malikova, N. P.; Tricoire, L.; Lambolez, B.; Vysotski, E. S.; Peyrieras, N.

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RedquorinXS Mutants with Enhanced Calcium Sensitivity and Bioluminescence Output Efficiently Report Cellular and Neuronal Network Activities / A. Bakayan, S. Picaud, N. P. Malikova [et al.] // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 21. - Ст. 7846, DOI 10.3390/ijms21217846. - Cited References:53. - This work was supported by grants from Centre National de la Recherche Scientifique (AAP Prematuration CNRS 2016, to A.B. and N.P.; equipment transfer to S.P. and B.L.), from Agence Nationale de la Recherche (AAP Prematuration FCS/IDEX Paris Saclay, to A.B. and N.P., France BioImaging infrastructure ANR-10-INBS-04, ANR-11-EQPX-029 to N.P.), from Fondation pour la Recherche sur le Cerveau/Rotary Club de France (B.L.), and from RFBR (project number 20-04-00085 to N.P.M. and E.S.V.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
IN-VIVO
   PHOTOPROTEIN AEQUORIN
   CA2+-REGULATED PHOTOPROTEINS
   SPREADING
Кл.слова (ненормированные):
bioluminescence -- aequorin -- calcium sensor -- BRET -- mutagenesis -- GPCR -- assay -- neuronal network imaging
Аннотация: Considerable efforts have been focused on shifting the wavelength of aequorin Ca2+-dependent blue bioluminescence through fusion with fluorescent proteins. This approach has notably yielded the widely used GFP-aequorin (GA) Ca2+ sensor emitting green light, and tdTomato-aequorin (Redquorin), whose bioluminescence is completely shifted to red, but whose Ca2+ sensitivity is low. In the present study, the screening of aequorin mutants generated at twenty-four amino acid positions in and around EF-hand Ca2+-binding domains resulted in the isolation of six aequorin single or double mutants (AequorinXS) in EF2, EF3, and C-terminal tail, which exhibited markedly higher Ca2+ sensitivity than wild-type aequorin in vitro. The corresponding Redquorin mutants all showed higher Ca2+ sensitivity than wild-type Redquorin, and four of them (RedquorinXS) matched the Ca2+ sensitivity of GA in vitro. RedquorinXS mutants exhibited unaltered thermostability and peak emission wavelengths. Upon stable expression in mammalian cell line, all RedquorinXS mutants reported the activation of the P2Y2 receptor by ATP with higher sensitivity and assay robustness than wt-Redquorin, and one, RedquorinXS-Q159T, outperformed GA. Finally, wide-field bioluminescence imaging in mouse neocortical slices showed that RedquorinXS-Q159T and GA similarly reported neuronal network activities elicited by the removal of extracellular Mg2+. Our results indicate that RedquorinXS-Q159T is a red light-emitting Ca2+ sensor suitable for the monitoring of intracellular signaling in a variety of applications in cells and tissues, and is a promising candidate for the transcranial monitoring of brain activities in living mice.

WOS
Держатели документа:
Ctr Natl Rech Sci CNRS, Inst Neurobiol Alfred Fessard, UPR 3294, Ave Terrasse, F-91198 Gif Sur Yvette, France.
Univ Paris Saclay, BioEmergences Unit, CNRS, USR 3695, Ave Terrasse, F-91198 Gif Sur Yvette, France.
Sorbonne Univ, Inst Biol Paris Seine NPS IBPS, INSERM, Neurosci Paris Seine,CNRS,UMR8246,U1130,UM119, F-75005 Paris, France.
Inst Biophys SB RAS, Fed Res Ctr, Photobiol Lab, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Bakayan, Adil; Picaud, Sandrine; Malikova, Natalia P.; Tricoire, Ludovic; Lambolez, Bertrand; Vysotski, Eugene S.; Peyrieras, Nadine; Vysotski, Eugene; Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR) [ANR-10-INBS-04, ANR-11-EQPX-029]; Fondation pour la Recherche sur le Cerveau/Rotary Club de France; RFBRRussian Foundation for Basic Research (RFBR) [20-04-00085]

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Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemistry. - 1995. - Vol. 34, Is. 10. - P3300-3309 . - ISSN 0006-2960
Кл.слова (ненормированные):
luciferase -- recombinant protein -- article -- ligand binding -- nonhuman -- priority journal -- protein isolation -- protein protein interaction -- protein stability -- vibrionaceae -- Bacterial Proteins -- Binding Sites -- Carrier Proteins -- Circular Dichroism -- Flavin Mononucleotide -- Fluorescence Polarization -- Genes, Bacterial -- Kinetics -- Ligands -- Luciferase -- Luminescence -- Molecular Sequence Data -- Photobacterium -- Recombinant Proteins -- Spectrophotometry -- Support, U.S. Gov't, P.H.S. -- Photobacterium leiognathi -- Vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.

Scopus
Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

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Phylogeny of Salmonoid Fishes (Salmonoidei) Based on mtDNA COI Gene Sequences (Barcoding) / V. S. Artamonova [et al.] // Contemp. Probl. Ecol. - 2018. - Vol. 11, Is. 3. - P271-285, DOI 10.1134/S1995425518030022. - Cited References:102. - We are very grateful to colleagues who helped collect samples: E.G. Berestovskii, I.N. Bolotov, E.A. Borovikova, I.V. Vikhrev, L.A. Glushchenko, V.V. Ignatenko, D.P. Karabanov, A.P. Novoselov, V.M. Spitsyn, V.A. Shirokov, and I.L. Shchurov; employees of Trout Hatchery "Adler", the Federal Breeding and Genetic Center for Fish Culture, and Vygsky and Kemsky fish hatcheries; and residents of Barabash-Levada, Len-lu, and Chupa settlements. We also thank S.S. Alekseev for identifying sharp-snouted and blunt-snouted lenoks. This work was supported by the Russian Science Foundation, project no. 16-14-10001. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
MOLECULAR DATING ANALYSIS
GROWTH-HORMONE INTRONS
SALMONIFORMES
Кл.слова (ненормированные):
evolution -- network -- molecular clock -- amino acid sequence -- reproductive -- isolation -- immobilization -- fishes
Аннотация: We have analyzed the partial sequences of the mitochondrial COI gene along with the amino acid sequences of cytochrome oxidase subunit I, encoded by this gene region, in representatives of 11 genera of salmonoid fish. For amino acid sequences, two alternative networks are constructed with outgroups represented by either Esocoidei or Osmeroidei as the supposed ancestral groups. This way, Osmeroidei appear to be closer to the salmonoid fish than Esocoidei, and their presence in the network as an outgroup explains the available data on the morphology and karyology of salmonoids much better. A number of the results of this study are fundamentally new. In particular, the slowing down of the molecular evolution of the grayling (Thymallidae) is shown. We conclude that the charr (Salvelinus) is one of the modern genera of salmonoids closest to their ancestor. The hypothesis of the phylogenetic proximity of the genera Brachymystax, Hucho, and Salmo has been confirmed. We also discuss the possibility that it is namely the changes in the amino acid sequence of cytochrome oxidase subunit I that lead to postzygotic reproductive isolation between taxa.

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Держатели документа:
Russian Acad Sci, Severtsov Inst Ecol & Evolut, Moscow 119071, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Artamonova, V. S.; Kolmakova, O. V.; Kirillova, E. A.; Makhrov, A. A.; Russian Science Foundation [16-14-10001]

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10.

Obtaining bacterial luciferase for bioluminescent analysis / V. S. Bondar [и др.] // Prikladnaya Biokhimiya i Mikrobiologiya. - 1988. - Vol. 24, Is. 6. - С. 745-753 . - ISSN 0555-1099
Кл.слова (ненормированные):
luciferase -- enzyme isolation -- enzyme subunit structure -- molecular weight -- nonhuman -- photobacterium leiognathi

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the USSR Academy of Sciences, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondar, V.S.; Vysotsky, E.S.; Zavoruev, V.V.; Mezhevikin, V.V.; Raibekas, A.A.

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11.

Novel Peptide Chemistry in Terrestrial Animals: Natural Luciferin Analogues from the Bioluminescent Earthworm Fridericia heliota [Text] / M. A. Dubinnyi [et al.] // Chem.-Eur. J. - 2015. - Vol. 21, Is. 10. - P3942-3947, DOI 10.1002/chem.201406498. - Cited References:17. - We thank Dr. K. V. Antonov for registration of LC-HRMS spectra. This work was supported by the Russian Science Foundation grant 14-50-00131. . - ISSN 0947-6539. - ISSN 1521-3765

РУБ Chemistry, Multidisciplinary
Рубрики:
STRUCTURE ELUCIDATION
DERIVATIVES
IDENTIFICATION
Кл.слова (ненормированные):
bioluminescence -- Fridericia heliota -- luciferin -- peptides -- structure -- elucidation
Аннотация: We report isolation and structure elucidation of AsLn5, AsLn7, AsLn11 and AsLn12: novel luciferin analogs from the bioluminescent earthworm Fridericia heliota. They were found to be highly unusual modified peptides, comprising either of the two tyrosine-derived chromophores, CompX or CompY and a set of amino acids, including threonine, gamma-aminobutyric acid, homoarginine, and unsymmetrical N,N-dimethylarginine. These natural compounds represent a unique peptide chemistry found in terrestrial animals and rise novel questions concerning their biosynthetic origin.

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Scopus
Держатели документа:
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow, Russian Federation
Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow, Russian Federation
Laboratory of Photobiology, Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Dubinnyi, Maxim A.; Tsarkova, Aleksandra S.; Petushkov, Valentin N.; Kaskova, Zinaida M.; Rodionova, Natalja S.; Kovalchuk, Sergey I.; Ziganshin, Rustam H.; Baranov, Mikhail S.; Mineev, Konstantin S.; Yampolsky, Ilia V.; Russian Science Foundation [14-50-00131]

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12.

Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of Lake Shira as an indicator of human impact on the ecosystem / T. I. Lobova [et al.] // Microbiological Research. - 2008. - Vol. 163, Is. 2. - P152-160, DOI 10.1016/j.micres.2006.03.014 . - ISSN 0944-5013
Кл.слова (ненормированные):
Antibiotic resistance -- Aquatic ecosystems -- Heterotrophic bacteria -- Human impact -- Monitoring -- Antibiotics -- Bacteria -- Ecosystems -- Environmental impact -- Mammals -- Antibiotic resistance -- Aquatic ecosystems -- Heterotrophic bacteria -- Materials -- antiinfective agent -- fresh water -- allochthony -- anthropogenic effect -- antibiotic resistance -- bacterium -- concentration (composition) -- heterotrophy -- intertidal environment -- monitoring -- recreational facility -- spring (season) -- summer -- animal -- article -- bacterial count -- bacterium -- drug effect -- ecosystem -- environmental monitoring -- heterotrophy -- human -- isolation and purification -- methodology -- microbiological examination -- microbiology -- multidrug resistance -- Russian Federation -- season -- Animals -- Anti-Bacterial Agents -- Bacteria -- Colony Count, Microbial -- Drug Resistance, Multiple, Bacterial -- Ecosystem -- Environmental Monitoring -- Fresh Water -- Heterotrophic Processes -- Humans -- Microbial Sensitivity Tests -- Russia -- Seasons -- Eurasia -- Khakassia -- Lake Shira -- Russian Federation -- Animalia -- Bacteria (microorganisms)
Аннотация: Resistance to Ampicillin and Kanamycin displayed by heterotrophic bacteria isolated in Summer and in Spring from the littoral and the central parts of Lake Shira (a therapeutic lake in the Khakasia Republic, Russia) has been investigated. It has been found that in Summer, human and animal microflora featuring multiple antibiotic resistance (to Ampicillin and Kanamycin) predominates in all the studied stations of the littoral zone of the lake. In Spring, concentrations of bacteria featuring multiple antibiotic resistance decrease significantly and bacteria sensitive to antibiotics predominate in the lake. Emergence of multiple antibiotic resistance in bacteria of Lake Shira is caused by the input of allochthonous bacteria into the lake; this feature of heterotrophic bacteria of Lake Shira can be used to monitor the impact on the ecosystem made by health resorts. В© 2006 Elsevier GmbH. All rights reserved.

Scopus
Держатели документа:
LTD Territory-oriented information systems, Institute of Computational Modeling, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok 50, 660036, Russian Federation
Center of Hygiene and Epidemiology in Krasnoyarsk Region, Sopochnaya 38, Russian Federation
Krasnoyarsk Scientific Centre, Akademgorodok 50, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Barkhatov, Y.V.; Salamatina, O.V.; Popova, L.Yu.

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13.

Microbiological and isotopic-geochemical investigations of meromictic lakes in Khakasia in winter / A. S. Savvichev [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 4. - С. 552-561 . - ISSN 0026-3656
Кл.слова (ненормированные):
Meromictic water bodies -- Microbial production and oxidation of methane -- Photosynthesis -- Stable isotopes of carbon (? 13C) and sulfur (? 34S) -- Sulfate reduction -- Bacteria (microorganisms) -- Chlorobi -- Chromatiaceae -- Lamprocystis purpurea -- Pelodictyon luteolum -- Photobacteria -- carbon -- fresh water -- methane -- sulfate -- sulfur -- article -- bacterial phenomena and functions -- bacterium -- comparative study -- isolation and purification -- metabolism -- microbiology -- oxidation reduction reaction -- photosynthesis -- Russian Federation -- season -- species difference -- Bacteria -- Bacterial Physiology -- Carbon Isotopes -- Fresh Water -- Methane -- Oxidation-Reduction -- Photosynthesis -- Seasons -- Siberia -- Species Specificity -- Sulfates -- Sulfur Isotopes -- Water Microbiology
Аннотация: Microbiological and isotopic-geochemical investigations of the brackish meromictic lakes Shira and Shunet were performed in the steppe region of Khakasia in winter. Measurements made with a submersed sensor demonstrated that one-meter ice transmits light in a quantity sufficient for oxygenic and anoxygenic photosynthesis. As in the summer season, in the community of phototrophic bacteria found in Lake Shira, the purple sulfur bacteria Amoebobacter purpureus dominated, whereas, in Lake Shunet, the green sulfur bacteria Pelodictyon luteolum were predominant. Photosynthetic production, measured using the radioisotopic method, was several times lower than that in summer. The rates of sulfate reduction and production and oxidation of methane in the water column and bottom sediments were also lower than those recorded in summer. The process of anaerobic methane oxidation in the sediments was an exception, being more intense in winter than in summer. The data from radioisotopic measurements of the rates of microbial processes correlate well with the results of determination of the isotopic composition of organic and mineral carbon (? 13C) and hydrogen sulfide and sulfate (? 34S) and suggest considerable seasonal variations in the activity of the microbial community in the water bodies investigated.

Scopus
Держатели документа:
Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya, Moscow, 117811, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 36, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Savvichev, A.S.; Rusanov, I.I.; Rogozin, D.Yu.; Zakharova, E.E.; Lunina, O.N.; Bryantseva, I.A.; Yusupov, S.K.; Pimenov, N.V.; Degermendzhi, A.G.; Ivanov, M.V.

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14.

    Isolation, Study and Application of Organosolv Lignins / B. N. Kuznetsov [и др.] // J. Sib. Fed. Univ.-Chem. - 2016. - Vol. 9, Is. 4. - С. 454-482, DOI 10.17516/1998-2836-2016-9-4-454-482. - Cited References:137 . - ISSN 1998-2836. - ISSN 2313-6049
Рубрики:
SIZE-EXCLUSION CHROMATOGRAPHY
   MOLECULAR-WEIGHT DISTRIBUTION
Кл.слова (ненормированные):
organosolv lignin -- isolation -- structure -- catalytic depolymerization -- molecular weight -- application -- liquid hydrocarbons -- aerogels
Аннотация: The analysis of the literature on the methods of soluble organosolv lignins isolation, their physical-chemical study and on the method of their processing to porous aerogels and liquid hydrocarbons was carried out. A review of the literature allowed us to choice of the most important areas of research. For isolation from wood the soluble lignins free from sulfur the methods of catalytic peroxide delignification at mild conditions (temperature <= 100 degrees C, atmospheric pressure) and methods of lignin extraction by supercritical organic solvents were used. Molecular mass and molecular-mass distribution of ethanol-lignin samples isolated from aspenwood and abies-wood were studied by gel-permeation chromatography. Weighted molecular mass of ethanol-lignin from abies wood is 478 Da and that from aspen wood ethanol-lignin - 750 Da. Thus, the studied samples of ethanol-lignin have rather low molecular mass, what should facilitate their further processing to liquid hydrocarbons and aerogels. For the depolymerization of organosolv lignins to liquid hydrocarbons the processes of their catalytic conversion in supercritical alcohols have good prospects for the use. In the processes of lignin thermal conversion alcohols can to extract the products of lignin depolymerization and to alkylate these products, preventing their repolymerization to high molecular mass substances. To obtain a new class of nanoporous materials based on lignin the methods of organic aerogels synthesis from mixtures of lignin with other natural polymers and crosslinking agents were applied. It was found that the structure and properties of porous materials of aerogel type depend not only from the reaction mixture composition but from the method of drying. Drying in subcritical conditions leads to the formation of xerogels, in supercritical conditions - to the formation aerogels and the freezdrying - of cryogels. Obtained porous materials can have very low density (around 0.2 g/cm(3)), high specific surface area (to 500 m(2)/g) and the pore volume near 5 cm(3)/g.

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Держатели документа:
FRC Krasnoyarsk Sci Ctr SB RAS, Inst Chem & Chem Technol SB RAS, 50-24 Akademgorodok, Krasnoyarsk 660036, Russia.
FRC Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Univ Lorraine, CNRS, UMR 7198, Inst Jean Lamour,ENSTIB, 27 Rue Philippe Seguin,CS 60036, F-88026 Epinal, France.

Доп.точки доступа:
Kuznetsov, Boris N.; Malyar, Yuriy N.; Kuznetsova, Svetlana A.; Grishechko, Lyudmila I.; Kazachenko, Alexander S.; Levdansky, Alexander V.; Pestunov, Andrey V.; Boyandin, Anatoly N.; Celzard, Alan

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15.

Isolation of luminescence system from the luminescent fungus Neonothopanus nambi [Text] / V. S. Bondar [et al.] // Dokl. Biochem. Biophys. - 2014. - Vol. 455, Is. 1. - P56-58, DOI 10.1134/S1607672914020045. - Cited References: 10. - This study was supported by the Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" (project no. 11, G34.31.0058) and the Siberian Branch of the Russian Academy of Sciences (project no. 71). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics

WOS
Держатели документа:
[Bondar, V. S.
Puzyr', A. P.
Purtov, K. V.
Petunin, A. I.
Rodicheva, E. K.
Medvedeva, S. E.
Gitel'zon, I. I.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Bondar, V. S.
Puzyr', A. P.
Purtov, K. V.
Burov, A. E.
Rodicheva, E. K.
Medvedeva, S. E.
Shpak, B. A.
Tyaglik, A. B.
Shimomura, O.
Gitel'zon, I. I.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Burov, A. E.] Russian Acad Sci, Nauka Special Design & Technol Bur, Krasnoyarsk Sci Ctr, Siberian Branch, Krasnoyarsk, Russia
[Shimomura, O.] Marine Biol Lab, Woods Hole, MA 02543 USA
ИБФ СО РАН
СКТБ Наука : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Bondar, V.S.; Puzyr', A.P.; Purtov, K.V.; Petunin, A.I.; Burov, A.E.; Rodicheva, E.K.; Medvedeva, S.E.; Shpak, B.A.; Tyaglik, A.B.; Shimomura, O...; Gitel'zon, I.I.; Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" [11, G34.31.0058]; Siberian Branch of the Russian Academy of Sciences [71]

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16.

17.

Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes / B. A. Illarrionov [et al.] // Gene. - 1990. - Vol. 86, Is. 1. - P89-94 . - ISSN 0378-1119
Кл.слова (ненормированные):
Bioluminescence -- expression in E. coli -- luciferase -- molecular evolution -- nucleotide sequence -- protein alignment -- recombinant DNA -- luciferase -- amino acid sequence -- article -- bioluminescence -- fungus -- gene structure -- genetic engineering -- heredity -- nonhuman -- nucleotide sequence -- priority journal -- vibrionaceae -- Acyltransferases -- Amino Acid Sequence -- Bacterial Proteins -- Base Sequence -- Cloning, Molecular -- DNA, Bacterial -- Genes, Structural, Bacterial -- Luciferase -- Luminescence -- Molecular Sequence Data -- Operon -- Photobacterium -- Restriction Mapping -- Escherichia coli -- Fungi -- Photobacterium leiognathi -- Vibrio harveyi -- Vibrionaceae
Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. В© 1990.

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
All-Union Research Institute of Molecular Biology, Novosibirsk Region, 633159, Russian Federation
Institute of Biophysics, Krasnoyarsk, 660036, Russian Federation
Institute of Clinical and Experimental Medicine, Novosibirsk, Russian Federation
Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, 630090, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Illarrionov, B.A.; Blinov, V.M.; Douchenko, A.P.; Protopopova, M.V.; Karginov, V.A.; Mertvetsov, N.P.; Gitelson, J.I.

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18.

Isolation and Purification of Fungal Luciferase from Neonothopanus nimbi / K. V. Purtov [et al.] // Dokl. Biochem. Biophys. - 2018. - Vol. 480, Is. 1. - P177-180, DOI 10.1134/S1607672918030134. - Cited References:6. - The study was supported by Russian Science Foundation Grant No. 16-14-00052. This research was carried out using the equipment provided by the Collective Use Center (CKP IBCH, ID of the agreement with Ministry of Education and Science of the Russian Federation: RFMEFI 62117X0018). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics

Аннотация: This is the first study to obtain a high-purity luciferase from the fungus Neonothopanus nambi bio-mass that is suitable for subsequent sequencing.

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Scopus
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, Inst Biophys, Krasnoyarsk 630036, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.

Доп.точки доступа:
Purtov, K. V.; Gorokhovatsky, A. Yu.; Kotlobay, A. A.; Osipova, Z. M.; Petushkov, V. N.; Rodionova, N. S.; Tsarkova, A. S.; Chepurnykh, T. V.; Yampolsky, I. V.; Gitelson, J. I.; Russian Science Foundation [16-14-00052]

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19.

ISOLATION AND PROPERTIES OF VARIOUS MOLECULAR-FORMS OF CA2+-ACTIVATED PHOTOPROTEIN OBELIN [Текст] / Y. S. VYSOTSKII, V. S. BONDAR, I. I. GITELZON // DOKLADY AKADEMII NAUK SSSR. - 1991. - Vol. 321, Is. 1. - С. 214-217. - Cited References: 14 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences
Рубрики:
CALCIUM-ACTIVATED PHOTOPROTEINS
   CTENOPHORES MNEMIOPSIS SP
   BEROE-OVATA
   AEQUORIN
   PROTEIN
   PURIFICATION
   EXTRACTION
   PHIALIDIN
   SEQUENCE
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VYSOTSKII, Y.S.; BONDAR, V.S.; GITELZON, I.I.

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20.

ISOLATION AND EXPRESSION OF CDNA CODING FOR PHOTOPROTEIN OBELIN FROM HYDROID OBELIA-LONGISSIMA [Текст] / B. A. ILLARIONOV [и др.] // Dokl. Akad. Nauk. - 1992. - Vol. 326, Is. 5. - С. 911-913. - Cited References: 12 . - 3. - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
AEQUORIN
   PROTEIN
   PHIALIDIN
   CLONING
   CA-2+
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
ILLARIONOV, B.A.; MARKOVA, S.V.; BONDAR, V.S.; VYSOTSKY, E.S.; GITELSON, J.I.

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