Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Найдено в других БД:

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (6)

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=isolation<.>)

Общее количество найденных документов : 33
Показаны документы с 1 по 20

1-20 21-33

Вид документа : Однотомное издание
Шифр издания : А.с. 1035962!-817652
Автор(ы) : Межевикин В.В., Высоцкий Е.С., Заворуев В.В., Родичева Э.К.
Заглавие : Способ получения препарата бактериальной люциферазы .-
Выходные данные : Б.м.,Б.г.
Коллективы : Ин-т биофиз. СО АН СССР
Цена : Б.ц.
ГРНТИ : 34.27.51
Предметные рубрики: ЛЮЦЕФЕРАЗА
ПОЛУЧЕНИЕ
СПОСОБ
PHOTOBACTERIUM LEIOGNATHI
БАКТЕРИИ
ENZYME ISOLATION
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bakayan A., Picaud S., Malikova N. P., Tricoire L., Lambolez B., Vysotski E. S., Peyrieras N.
Заглавие : Redquorinxs mutants with enhanced calcium sensitivity and bioluminescence output efficiently report cellular and neuronal network activities
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2020. - Vol. 21, Is. 21. - Ст.7846. - С. 1-22. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21217846
Аннотация: Considerable efforts have been focused on shifting the wavelength of aequorin Ca2+? dependent blue bioluminescence through fusion with fluorescent proteins. This approach has notably yielded the widely used GFP?aequorin (GA) Ca2+ sensor emitting green light, and tdTomato-aequorin (Redquorin), whose bioluminescence is completely shifted to red, but whose Ca2+ sensitivity is low. In the present study, the screening of aequorin mutants generated at twenty?four amino acid positions in and around EF?hand Ca2+?binding domains resulted in the isolation of six aequorin single or double mutants (AequorinXS) in EF2, EF3, and C?terminal tail, which exhibited markedly higher Ca2+ sensitivity than wild?type aequorin in vitro. The corresponding Redquorin mutants all showed higher Ca2+ sensitivity than wild?type Redquorin, and four of them (RedquorinXS) matched the Ca2+ sensitivity of GA in vitro. RedquorinXS mutants exhibited unaltered thermostability and peak emission wavelengths. Upon stable expression in mammalian cell line, all RedquorinXS mutants reported the activation of the P2Y2 receptor by ATP with higher sensitivity and assay robustness than wt?Redquorin, and one, RedquorinXS?Q159T, outperformed GA. Finally, wide?field bioluminescence imaging in mouse neocortical slices showed that RedquorinXS?Q159T and GA similarly reported neuronal network activities elicited by the removal of extracellular Mg2+. Our results indicate that RedquorinXS?Q159T is a red light?emitting Ca2+ sensor suitable for the monitoring of intracellular signaling in a variety of applications in cells and tissues, and is a promising candidate for the transcranial monitoring of brain activities in living mice. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bakayan, Adil, Picaud, Sandrine, Malikova, Natalia P., Tricoire, Ludovic, Lambolez, Bertrand, Vysotski, Eugene S., Peyrieras, Nadine
Заглавие : RedquorinXS Mutants with Enhanced Calcium Sensitivity and Bioluminescence Output Efficiently Report Cellular and Neuronal Network Activities
Колич.характеристики :20 с
Коллективы : Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR) [ANR-10-INBS-04, ANR-11-EQPX-029]; Fondation pour la Recherche sur le Cerveau/Rotary Club de France; RFBRRussian Foundation for Basic Research (RFBR) [20-04-00085]
Место публикации : Int. J. Mol. Sci.: MDPI, 2020. - Vol. 21, Is. 21. - Ст.7846. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms21217846
Примечания : Cited References:53. - This work was supported by grants from Centre National de la Recherche Scientifique (AAP Prematuration CNRS 2016, to A.B. and N.P.; equipment transfer to S.P. and B.L.), from Agence Nationale de la Recherche (AAP Prematuration FCS/IDEX Paris Saclay, to A.B. and N.P., France BioImaging infrastructure ANR-10-INBS-04, ANR-11-EQPX-029 to N.P.), from Fondation pour la Recherche sur le Cerveau/Rotary Club de France (B.L.), and from RFBR (project number 20-04-00085 to N.P.M. and E.S.V.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Предметные рубрики: IN-VIVO
PHOTOPROTEIN AEQUORIN
CA2+-REGULATED PHOTOPROTEINS
SPREADING
Аннотация: Considerable efforts have been focused on shifting the wavelength of aequorin Ca2+-dependent blue bioluminescence through fusion with fluorescent proteins. This approach has notably yielded the widely used GFP-aequorin (GA) Ca2+ sensor emitting green light, and tdTomato-aequorin (Redquorin), whose bioluminescence is completely shifted to red, but whose Ca2+ sensitivity is low. In the present study, the screening of aequorin mutants generated at twenty-four amino acid positions in and around EF-hand Ca2+-binding domains resulted in the isolation of six aequorin single or double mutants (AequorinXS) in EF2, EF3, and C-terminal tail, which exhibited markedly higher Ca2+ sensitivity than wild-type aequorin in vitro. The corresponding Redquorin mutants all showed higher Ca2+ sensitivity than wild-type Redquorin, and four of them (RedquorinXS) matched the Ca2+ sensitivity of GA in vitro. RedquorinXS mutants exhibited unaltered thermostability and peak emission wavelengths. Upon stable expression in mammalian cell line, all RedquorinXS mutants reported the activation of the P2Y2 receptor by ATP with higher sensitivity and assay robustness than wt-Redquorin, and one, RedquorinXS-Q159T, outperformed GA. Finally, wide-field bioluminescence imaging in mouse neocortical slices showed that RedquorinXS-Q159T and GA similarly reported neuronal network activities elicited by the removal of extracellular Mg2+. Our results indicate that RedquorinXS-Q159T is a red light-emitting Ca2+ sensor suitable for the monitoring of intracellular signaling in a variety of applications in cells and tissues, and is a promising candidate for the transcranial monitoring of brain activities in living mice.
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Joseph, Blessy, Sam, Rubie Mavelil, Balakrishnan, Preetha, Maria, Hanna J., Gopi, Sreeraj, Volova, Tatiana, Fernandes, Susana C. M., Thomas, Sabu
Заглавие : Extraction of Nanochitin from Marine Resources and Fabrication of Polymer Nanocomposites: Recent Advances
Колич.характеристики :37 с
Коллективы : Government of the Russian Federation [074-02-2018-328, 220]; "Investissements d'Avenir" French programFrench National Research Agency (ANR) [ANR-16-IDEX-0002]; Region Nouvelle-AquitaineRegion Nouvelle-Aquitaine; Communaute d'Agglomeration du Pays Basque, France
Место публикации : Polymers: MDPI, 2020. - Vol. 12, Is. 8. - Ст.1664. - ISSN 2073-4360(eISSN), DOI 10.3390/polym12081664
Примечания : Cited References:128. - This study was financially supported by project "Agro preparations of the new generation: a strategy of construction and realization" (agreement number 074-02-2018-328) in accordance with resolution number 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." S.C.M.F. is the recipient of an E2S UPPA Research Partnership Chair (MANTA: Marine Materials) supported by the "Investissements d'Avenir" French program managed by ANR (ANR-16-IDEX-0002), the Region Nouvelle-Aquitaine and the Communaute d'Agglomeration du Pays Basque, France.
Предметные рубрики: NATURAL-RUBBER NANOCOMPOSITES
ELECTROSPUN PVDF MEMBRANE
ALPHA-CHITIN
Аннотация: Industrial sea food residues, mainly crab and shrimp shells, are considered to be the most promising and abundant source of chitin. In-depth understanding of the biological properties of chitin and scientific advancements in the field of nanotechnology have enabled the development of high-performance chitin nanomaterials. Nanoscale chitin is of great economic value as an efficient functional and reinforcement material for a wide range of applications ranging from water purification to tissue engineering. The use of polymers and nanochitin to produce (bio) nanocomposites offers a good opportunity to prepare bioplastic materials with enhanced functional and structural properties. Most processes for nanochitin isolation rely on the use of chemical, physical or mechanical methods. Chitin-based nanocomposites are fabricated by various methods, involving electrospinning, freeze drying, etc. This review discusses the progress and new developments in the isolation and physico-chemical characterization of chitin; it also highlights the processing of nanochitin in various composite and functional materials.
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Mogilnaya O. A., Artemenko K. S., Posokhina E. D., Bondar V. S.
Заглавие : Extracellular Oxidases of Basidiomycete Neonothopanus nambi: Isolation and Some Properties
Колич.характеристики :5 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 490, Is. 1. - С. 38-42. - ISSN 1607-6729, DOI 10.1134/S1607672920010135. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15
Предметные рубрики: PEROXIDASE-ACTIVITY
LIGHT-EMISSION
Аннотация: Using the original technique of treating biomass with beta-glucosidase, a pool of extracellular fungal enzymes was obtained for the first time from the mycelium of basidiomycete Neonothopanus nambi. Two protein fractions containing enzymes with oxidase activity were isolated from the extract by gel-filtration chromatography and conventionally called F1 and F2. Enzyme F1 has a native molecular weight of 80-85 kDa and does not contain chromophore components; however, it catalyzes the oxidation of veratryl alcohol with K-m = 0.52 mM. Probably, this enzyme is an alcohol oxidase. Enzyme F2 with a native molecular weight of approximately 60 kDa is a FAD-containing protein. It catalyzes the cooxidation of phenol with 4-aminoantipyrine without the addition of exogenous hydrogen peroxide, which distinguishes it from the known peroxidases. It was assumed that this enzyme may be a mixed-function oxidase. F2 oxidase has K-m value 0.27 mM for phenol. The temperature optimums for oxidases F1 and F2 are 22-35 and 55-70 degrees C, and pH optimums are 6 and 5, respectively.
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Efremov A. A., Zykova I. D., Korosteleva N. S.
Заглавие : Antimicrobial and antiradical activity of individual fractions of essential oil from seeds of heracleum dissectum ledeb. Of Siberian Region
Место публикации : Khimiya Rastitel'nogo Syr'ya: Altai State University, 2020. - Is. 2. - С. 79-85. - ISSN 10295151 (ISSN), DOI 10.14258/JCPRM.2020027029
Аннотация: By the method of exhaustive hydroponically obtained essential oil from beans of Heracleum dissectum Ledeb., growing in the Krasnoyarsk region. Separate fractions of oil were obtained: the first after 45 minutes from the beginning of distillation, the second – after 2 hours, the third-after 5 hours, the fourth fraction was collected after the end of hydro-distillation. The component composition of both whole essential oil and its separate fractions was studied. The main components are octyl acetate (60.0%), octyl-2-methylpropanoate (10.2%), n-hexyl-2-methylbutanoate (9.0%). The main amount of octyl acetate (64.7%) is concentrated in the first fraction of the oil. The antimicrobial activity of various fractions of essential oil of borscht dissected against strains of opportunistic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, MRSA, Proteus vulgaris. It was found that, depending on the duration of isolation, the antimicrobial activity of essential oil fractions in relation to Staphylococcus aureus 209p, MRSA and Pseudomonas aeruginosa decreases, and in relation to Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris increases. The most pronounced inhibitory effect of the third and fourth fractions of essential oil against Klebsiella pneumonia. The antiradical activity of all studied samples of borscht essential oil dissected in reaction with stable free 2,2-diphenyl-1-picrylhydrazyl radical was established. The first fraction showed minimal antiradical activity (15.1%), the fourth – maximum (49.2%). © 2020 Altai State University. All rights reserved.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Artamonova V. S., Kolmakova O. V., Kirillova E. A., Makhrov A. A.
Заглавие : Phylogeny of Salmonoid Fishes (Salmonoidei) Based on mtDNA COI Gene Sequences (Barcoding)
Колич.характеристики :15 с
Коллективы : Russian Science Foundation [16-14-10001]
Место публикации : Contemp. Probl. Ecol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2018. - Vol. 11, Is. 3. - С. 271-285. - ISSN 1995-4255, DOI 10.1134/S1995425518030022. - ISSN 1995-4263(eISSN)
Примечания : Cited References:102. - We are very grateful to colleagues who helped collect samples: E.G. Berestovskii, I.N. Bolotov, E.A. Borovikova, I.V. Vikhrev, L.A. Glushchenko, V.V. Ignatenko, D.P. Karabanov, A.P. Novoselov, V.M. Spitsyn, V.A. Shirokov, and I.L. Shchurov; employees of Trout Hatchery "Adler", the Federal Breeding and Genetic Center for Fish Culture, and Vygsky and Kemsky fish hatcheries; and residents of Barabash-Levada, Len-lu, and Chupa settlements. We also thank S.S. Alekseev for identifying sharp-snouted and blunt-snouted lenoks. This work was supported by the Russian Science Foundation, project no. 16-14-10001.
Предметные рубрики: MOLECULAR DATING ANALYSIS
GROWTH-HORMONE INTRONS
SALMONIFORMES
Ключевые слова (''Своб.индексиров.''): evolution--network--molecular clock--amino acid sequence--reproductive--isolation--immobilization--fishes
Аннотация: We have analyzed the partial sequences of the mitochondrial COI gene along with the amino acid sequences of cytochrome oxidase subunit I, encoded by this gene region, in representatives of 11 genera of salmonoid fish. For amino acid sequences, two alternative networks are constructed with outgroups represented by either Esocoidei or Osmeroidei as the supposed ancestral groups. This way, Osmeroidei appear to be closer to the salmonoid fish than Esocoidei, and their presence in the network as an outgroup explains the available data on the morphology and karyology of salmonoids much better. A number of the results of this study are fundamentally new. In particular, the slowing down of the molecular evolution of the grayling (Thymallidae) is shown. We conclude that the charr (Salvelinus) is one of the modern genera of salmonoids closest to their ancestor. The hypothesis of the phylogenetic proximity of the genera Brachymystax, Hucho, and Salmo has been confirmed. We also discuss the possibility that it is namely the changes in the amino acid sequence of cytochrome oxidase subunit I that lead to postzygotic reproductive isolation between taxa.
WOS,
Смотреть статью,
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K. V., Gorokhovatsky, A. Yu., Kotlobay A. A., Osipova Z. M., Petushkov V. N., Rodionova N. S., Tsarkova A. S., Chepurnykh T. V., Yampolsky I. V., Gitelson J. I.
Заглавие : Isolation and Purification of Fungal Luciferase from Neonothopanus nimbi
Колич.характеристики :4 с
Коллективы : Russian Science Foundation [16-14-00052]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2018. - Vol. 480, Is. 1. - С. 177-180. - ISSN 1607-6729, DOI 10.1134/S1607672918030134. - ISSN 1608-3091(eISSN)
Примечания : Cited References:6. - The study was supported by Russian Science Foundation Grant No. 16-14-00052. This research was carried out using the equipment provided by the Collective Use Center (CKP IBCH, ID of the agreement with Ministry of Education and Science of the Russian Federation: RFMEFI 62117X0018).
Аннотация: This is the first study to obtain a high-purity luciferase from the fungus Neonothopanus nambi bio-mass that is suitable for subsequent sequencing.
WOS,
Смотреть статью,
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kuznetsov, Boris N., Malyar, Yuriy N., Kuznetsova, Svetlana A., Grishechko, Lyudmila I., Kazachenko, Alexander S., Levdansky, Alexander V., Pestunov, Andrey V., Boyandin, Anatoly N., Celzard, Alan
Заглавие : Isolation, Study and Application of Organosolv Lignins
Колич.характеристики :29 с
Место публикации : J. Sib. Fed. Univ.-Chem.: SIBERIAN FEDERAL UNIV, 2016. - Vol. 9, Is. 4. - С. 454-482. - ISSN 1998-2836, DOI 10.17516/1998-2836-2016-9-4-454-482. - ISSN 2313-6049(eISSN)
Примечания : Cited References:137
Предметные рубрики: SIZE-EXCLUSION CHROMATOGRAPHY
MOLECULAR-WEIGHT DISTRIBUTION
Ключевые слова (''Своб.индексиров.''): organosolv lignin--isolation--structure--catalytic depolymerization--molecular weight--application--liquid hydrocarbons--aerogels
Аннотация: The analysis of the literature on the methods of soluble organosolv lignins isolation, their physical-chemical study and on the method of their processing to porous aerogels and liquid hydrocarbons was carried out. A review of the literature allowed us to choice of the most important areas of research. For isolation from wood the soluble lignins free from sulfur the methods of catalytic peroxide delignification at mild conditions (temperature
WOS,
Смотреть статью
Найти похожие

10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Bondar V. S.
Заглавие : The use of glowing wood as a source of luminescent culture of fungus mycelium
Колич.характеристики :17 с
Коллективы : RF Government [11.G34.31.0058]; SB RAS [71]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2016. - Vol. 7, Is. 1. - С. 1-17. - ISSN 2077-7000, DOI 10.5943/mycosphere/7/1/1
Примечания : Cited References:22. - The authors are grateful to Prof. A. Frank, Director of North Borneo Biostation, for the opportunity to carry out studies of glowing wood; to Nadezhda N. Kudashova, a senior researcher at the Institute of Biology and Biophysics at the Tomsk University, for identifying the species of nonluminous fungi. This study was supported by grant no. 11.G34.31.0058 (RF Government) and Projects no. 71 (SB RAS).
Предметные рубрики: BIOLUMINESCENCE CHARACTERISTICS
NEONOTHOPANUS-NAMBI
LIGHT-EMISSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--culture of luminous mycelia--kinetics of luminescent--reaction--light emitting wood--luminous fungus
Аннотация: In studies of fungal bioluminescence, not only fruiting bodies and spores of the fungus, but also samples of luminescent wood, leaf litter or soil may need to be used to derive pure mycelial culture. This study describes an approach to isolating the culture of luminescent fungal mycelium from samples of light-emitting wood found on Borneo Island in November-December 2013. A GelDoc XR Imaging System (Bio-Rad Laboratories, Inc., U.S.) was used for the first time to monitor luminescence and select luminous samples. This study shows that for successful isolation of the culture of luminescent mycelium out of the luminescent wood found in the forest, it is imperative to keep the samples moist (mycelium alive until there is water), while immediate and aseptic delivery of the samples to the laboratory is not a crucial condition (inner layers of wood is "sterile"). Investigation of the growth features of the isolated mycelium in various growing conditions revealed some peculiar properties of its luminescence in comparison with the known luminescent cultures of basidiomycetes. When grown on solid nutrient media, mycelium exhibits low growth rates, long-lasting luminescence (140 days or longer), and emergence and disappearance of local zones with high levels of light emission. Mycelium produced in submerged culture does not emit light, and this effect must be caused by the absence or a very low level of the luminescent reaction substrate in the biomass. The luminescence system isolated from mycelial biomass did not induce luminescent reaction in vitro upon the addition of NADPH (recording intensity is 60 100 URL/sec). We found that enzymes of the luminescence systems isolated from mycelium pellets retained their activity and catalyzed luminescent reaction when a hot extract of the luminous fungus Armillaria sp. (IBSO 2360) was added (near 1900 URL/sec). The same effect was obtained after addition of hot extracts from the fruiting bodies of nonluminous higher fungi Pholiota squarrosa, Cortinarius sp., Hypholoma capnoides and Chroogomphus rutilus (near 3500 URL/sec). The pure culture of luminescent mycelium has been registered in the Culture Collection of IBP SB RAS as IBSO 2371; now it can be used for various in vivo and in vitro studies, including identification of the fungus.
WOS,
Смотреть статью
Найти похожие

11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi, Maxim A., Tsarkova, Aleksandra S., Petushkov, Valentin N., Kaskova, Zinaida M., Rodionova, Natalja S., Kovalchuk, Sergey I., Ziganshin, Rustam H., Baranov, Mikhail S., Mineev, Konstantin S., Yampolsky, Ilia V.
Заглавие : Novel Peptide Chemistry in Terrestrial Animals: Natural Luciferin Analogues from the Bioluminescent Earthworm Fridericia heliota
Колич.характеристики :6 с
Коллективы : Russian Science Foundation [14-50-00131]
Место публикации : Chem.-Eur. J.: WILEY-V C H VERLAG GMBH, 2015. - Vol. 21, Is. 10. - С. 3942-3947. - ISSN 0947-6539, DOI 10.1002/chem.201406498. - ISSN 1521-3765(eISSN)
Примечания : Cited References:17. - We thank Dr. K. V. Antonov for registration of LC-HRMS spectra. This work was supported by the Russian Science Foundation grant 14-50-00131.
Предметные рубрики: STRUCTURE ELUCIDATION
DERIVATIVES
IDENTIFICATION
Ключевые слова (''Своб.индексиров.''): bioluminescence--fridericia heliota--luciferin--peptides--structure--elucidation
Аннотация: We report isolation and structure elucidation of AsLn5, AsLn7, AsLn11 and AsLn12: novel luciferin analogs from the bioluminescent earthworm Fridericia heliota. They were found to be highly unusual modified peptides, comprising either of the two tyrosine-derived chromophores, CompX or CompY and a set of amino acids, including threonine, gamma-aminobutyric acid, homoarginine, and unsymmetrical N,N-dimethylarginine. These natural compounds represent a unique peptide chemistry found in terrestrial animals and rise novel questions concerning their biosynthetic origin.
WOS,
Scopus
Найти похожие

12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Puzyr' A.P., Purtov K.V., Petunin A.I., Burov A.E., Rodicheva E.K., Medvedeva S.E., Shpak B.A., Tyaglik A.B., Shimomura O..., Gitel'zon I.I.
Заглавие : Isolation of luminescence system from the luminescent fungus Neonothopanus nambi
Колич.характеристики :3 с
Коллективы : Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" [11, G34.31.0058]; Siberian Branch of the Russian Academy of Sciences [71]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2014. - Vol. 455, Is. 1. - С. 56-58. - ISSN 1607-6729, DOI 10.1134/S1607672914020045. - ISSN 1608-3091
Примечания : Cited References: 10. - This study was supported by the Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" (project no. 11, G34.31.0058) and the Siberian Branch of the Russian Academy of Sciences (project no. 71).
WOS
Найти похожие

13.

14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Burakova L.P., Krasitskaya V.V., Kudryavtsev A.N., Shimomura O..., Frank L.A.
Заглавие : Hydrogen-bond networks between the C-terminus and Arg from the first alpha-helix stabilize photoprotein molecules
Колич.характеристики :7 с
Коллективы : RFBR [12-04-00753-a]; Government of Russian Federation [11.G34.31.0058]
Место публикации : Photochem. Photobiol. Sci.: ROYAL SOC CHEMISTRY, 2014. - Vol. 13, Is. 3. - С. 541-547. - ISSN 1474-905X, DOI 10.1039/c3pp50369k. - ISSN 1474-9092
Примечания : Cited References: 22. - The work was supported by RFBR grant 12-04-00753-a, by the Program of the Government of Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.0058).
Предметные рубрики: GREEN FLUORESCENT PROTEIN
CA2+-REGULATED PHOTOPROTEIN
BIOLUMINESCENT IMMUNOASSAY
COELENTERAZINE BINDING
ANGSTROM RESOLUTION
ENERGY-TRANSFER
FUSION PROTEIN
APO-OBELIN
AEQUORIN
EXPRESSION
Аннотация: Previous studies have stated that aequorin loses most of its bioluminescence activity upon modification of the C-terminus, thus limiting the production of photoprotein fusion proteins at its N-terminus. In the present work, we investigate the importance of the C-terminal proline and the hydrogen bonds it forms for photoprotein active complex formation, stability and functional activity. According to the crystal structures of obelin and aequorin, two Ca2+-regulated photoproteins, the carboxyl group of the C-terminal Pro forms two hydrogen bonds with the side chain of Arg21 (Arg15 in aequorin case) situated in the first a-helix. Whereas, deletion or substitution of the C-terminal proline could noticeably change the bioluminescence activity, stability or the yield of an active photoprotein complex. Therefore, modifications of the first alpha-helix Arg has a clear destructive effect on the main photoprotein properties. A C-terminal hydrogen-bond network is proposed to be important for the stability of photoprotein molecules towards external disturbances, when taking part in the formation of locked protein conformations and isolation of coelenterazine-binding cavities.
WOS
Найти похожие

15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A., Borisova V.V., Vereshchagina T.A., Fomenko E.V., Anshits A.G., Gitelson I.I.
Заглавие : Affine magnetic sorbents supported on coal ash microspheres for recombinant protein isolation
Место публикации : Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 2. - С. 215-220. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683809020173
Ключевые слова (''Своб.индексиров.''): clytia gregaria--obelia longissima
Аннотация: The results of the development and utilization of an affine magnetic sorbent with Ni2+ ions immobilized on coal ash microspheres are reported. The applicability of the material in the isolation of Histag proteins is demonstrated by examples of the recombinant green fluorescent protein from Clytia gregaria and the Ca2+ regulated photoprotein obelin from Obelia longissima. The specific sorption capacity of the sorbent was 2-7 mg/cm3 for medium-size proteins (20-30 kDa). The particles are suitable for chromatography with the presence of chaotropic agents and EDTA. They are easy to manipulate as isolation of a target protein takes 30-35 min. On the one hand, the elevated affinity of the sorbent to proteins rich in native histidines may result in a high degree of irreversible sorption; on the other hand, it allows isolation of such proteins without the introduction of artificial polyhistidine fragments. В© 2009 Pleiades Publishing, Ltd.
Scopus
Найти похожие

16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lobova T.I., Barkhatov Y.V., Salamatina O.V., Popova L.Yu.
Заглавие : Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of Lake Shira as an indicator of human impact on the ecosystem
Место публикации : Microbiological Research. - 2008. - Vol. 163, Is. 2. - С. 152-160. - ISSN 09445013 (ISSN) , DOI 10.1016/j.micres.2006.03.014
Ключевые слова (''Своб.индексиров.''): antibiotic resistance--aquatic ecosystems--heterotrophic bacteria--human impact--monitoring--antibiotics--bacteria--ecosystems--environmental impact--mammals--antibiotic resistance--aquatic ecosystems--heterotrophic bacteria--materials--antiinfective agent--fresh water--allochthony--anthropogenic effect--antibiotic resistance--bacterium--concentration (composition)--heterotrophy--intertidal environment--monitoring--recreational facility--spring (season)--summer--animal--article--bacterial count--bacterium--drug effect--ecosystem--environmental monitoring--heterotrophy--human--isolation and purification--methodology--microbiological examination--microbiology--multidrug resistance--russian federation--season--animals--anti-bacterial agents--bacteria--colony count, microbial--drug resistance, multiple, bacterial--ecosystem--environmental monitoring--fresh water--heterotrophic processes--humans--microbial sensitivity tests--russia--seasons--eurasia--khakassia--lake shira--russian federation--animalia--bacteria (microorganisms)
Аннотация: Resistance to Ampicillin and Kanamycin displayed by heterotrophic bacteria isolated in Summer and in Spring from the littoral and the central parts of Lake Shira (a therapeutic lake in the Khakasia Republic, Russia) has been investigated. It has been found that in Summer, human and animal microflora featuring multiple antibiotic resistance (to Ampicillin and Kanamycin) predominates in all the studied stations of the littoral zone of the lake. In Spring, concentrations of bacteria featuring multiple antibiotic resistance decrease significantly and bacteria sensitive to antibiotics predominate in the lake. Emergence of multiple antibiotic resistance in bacteria of Lake Shira is caused by the input of allochthonous bacteria into the lake; this feature of heterotrophic bacteria of Lake Shira can be used to monitor the impact on the ecosystem made by health resorts. В© 2006 Elsevier GmbH. All rights reserved.
Scopus
Найти похожие

17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Savvichev A.S., Rusanov I.I., Rogozin D.Yu., Zakharova E.E., Lunina O.N., Bryantseva I.A., Yusupov S.K., Pimenov N.V., Degermendzhi A.G., Ivanov M.V.
Заглавие : Microbiological and isotopic-geochemical investigations of meromictic lakes in Khakasia in winter
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 4. - С. 552-561. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): meromictic water bodies--microbial production and oxidation of methane--photosynthesis--stable isotopes of carbon (? 13c) and sulfur (? 34s)--sulfate reduction--bacteria (microorganisms)--chlorobi--chromatiaceae--lamprocystis purpurea--pelodictyon luteolum--photobacteria--carbon--fresh water--methane--sulfate--sulfur--article--bacterial phenomena and functions--bacterium--comparative study--isolation and purification--metabolism--microbiology--oxidation reduction reaction--photosynthesis--russian federation--season--species difference--bacteria--bacterial physiology--carbon isotopes--fresh water--methane--oxidation-reduction--photosynthesis--seasons--siberia--species specificity--sulfates--sulfur isotopes--water microbiology
Аннотация: Microbiological and isotopic-geochemical investigations of the brackish meromictic lakes Shira and Shunet were performed in the steppe region of Khakasia in winter. Measurements made with a submersed sensor demonstrated that one-meter ice transmits light in a quantity sufficient for oxygenic and anoxygenic photosynthesis. As in the summer season, in the community of phototrophic bacteria found in Lake Shira, the purple sulfur bacteria Amoebobacter purpureus dominated, whereas, in Lake Shunet, the green sulfur bacteria Pelodictyon luteolum were predominant. Photosynthetic production, measured using the radioisotopic method, was several times lower than that in summer. The rates of sulfate reduction and production and oxidation of methane in the water column and bottom sediments were also lower than those recorded in summer. The process of anaerobic methane oxidation in the sediments was an exception, being more intense in winter than in summer. The data from radioisotopic measurements of the rates of microbial processes correlate well with the results of determination of the isotopic composition of organic and mineral carbon (? 13C) and hydrogen sulfide and sulfate (? 34S) and suggest considerable seasonal variations in the activity of the microbial community in the water bodies investigated.
Scopus
Найти похожие

18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S.E., Boyandin A., Lankin Y., Kotov D., Rodicheva E., Popova L.
Заглавие : BIOLUMBASE - The database of natural and transgenic bioluminescent organisms
Место публикации : Luminescence. - 2005. - Vol. 20, Is. 2. - С. 90-96. - ISSN 15227235 (ISSN) , DOI 10.1002/bio.809
Ключевые слова (''Своб.индексиров.''): bioluminescence--database--luminous bacteria--lux gene--marine--article--bacterial strain--bacterium culture--bacterium isolation--bioluminescence--data base--gene construct--medical information--transgenics--wide area network--bacteria--bacterial proteins--databases, factual--ecology--luminescence--luminescent proteins--marine biology--organisms, genetically modified--photobacterium--transgenes
Аннотация: The Institute of Biophysics SB RAS hosts and maintains a specialized collection of luminous bacteria (CCIBSO 836) containing over 700 strains isolated in various regions of the world's oceans. The culture collection is a source of lux genes and biologically active substances. The wide application of bioluminescence in medicine and ecology has given importance to analys-ing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as on their features that are closely interrelated with bioluminescence. The aims of our BIOLUMBASE database are: gathering information on microorganisms with lux genes, their analysis and free access, and distribution of this data throughout the global network. The database includes two sections, natural and transgenic luminous microorganisms, and is updated by our own experimental results, the published literature and internet resources. For the future, a publicly available internet site for BIOLUMBASE is planned. This will list the strains and provide comprehensive information on the properties and functions of luminous bacteria, the mechanisms of regulation of bioluminescence systems, constructs with lux genes, and applications of bioluminescence in microbiology, ecology, medicine and biotechnology. It is noteworthy that this database will also be useful for evaluation of biological hazards of transgenic strains. Users will be able to carry out bibliographic and strain searches starting from any feature of interest. Copyright В© 2005 John Wiley & Sons, Ltd.
Scopus
Найти похожие

19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tolomeyev A.P., Zadereev Y.S.
Заглавие : An in situ method for the investigation of vertical distributions of zooplankton in lakes: Test of a two-compartment enclosure
Место публикации : Aquatic Ecology. - 2005. - Vol. 39, Is. 1-2. - С. 181-188. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-004-5732-0
Ключевые слова (''Своб.индексиров.''): anoxic hypolimnion--solar radiation--stratified lake--vertical migration--zooplankton--biotic factor--enclosure experiment--lake ecosystem--physicochemical property--vertical distribution--zooplankton--animalia--arctodiaptomus salinus--brachionus plicatilis
Аннотация: Two-section enclosures were designed for the investigation of the effect of various physicochemical and biological factors on vertical distribution of zooplankton in situ. The framework of the enclosure was a cylindrical polyethylene column without any partitions inside, in which the isolation of animals in different sections after in situ exposure was achieved by pinching the flexible central part of the column. Enclosures were tested at the brackish stratified meromictic Lake Shira (Russia, Khakasia). The absence of fish and carnivorous zooplankton in the lake suggests that the vertical distribution of zooplankton is mainly determined by physicochemical gradients in the water column. Experiments and field observations demonstrated that all age and size groups of Arctodiaptomus salinus and Brachionus plicatilis strongly avoided surface layers during the daylight. The escape of zooplankton from the anoxic hypolimnion was less active. Statistically significant avoidance was observed only for copepodites C4-C5 and females of A. salinus. The relatively simple construction of the columns and easy handling during the experiment were the factors that favoured the use of this device to perform in situ basic tests of the effect of different factors on the vertical distribution of zooplankton. В© Springer 2005.
Scopus
Найти похожие

20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trusova M.Yu., Gladyshev M.I.
Заглавие : Species composition of winter bacterioplankton in blooming and nonblooming reservoirs as determined by 16S rRNA sequences
Место публикации : Doklady Biological Sciences. - 2005. - Vol. 405, Is. 1-6. - С. 443-445. - ISSN 00124966 (ISSN) , DOI 10.1007/s10630-005-0160-4
Ключевые слова (''Своб.индексиров.''): fresh water--rna 16s--article--bacterium--classification--genetic variability--genetics--isolation and purification--microbiology--molecular genetics--phylogeny--plankton--russian federation--season--species difference--bacteria--fresh water--molecular sequence data--phylogeny--plankton--rna, ribosomal, 16s--seasons--siberia--species specificity--variation (genetics)--water microbiology
Scopus
Найти похожие