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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Savvichev A.S., Rusanov I.I., Rogozin D.Yu., Zakharova E.E., Lunina O.N., Bryantseva I.A., Yusupov S.K., Pimenov N.V., Degermendzhi A.G., Ivanov M.V.
Заглавие : Microbiological and isotopic-geochemical investigations of meromictic lakes in Khakasia in winter
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 4. - С. 552-561. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): meromictic water bodies--microbial production and oxidation of methane--photosynthesis--stable isotopes of carbon (? 13c) and sulfur (? 34s)--sulfate reduction--bacteria (microorganisms)--chlorobi--chromatiaceae--lamprocystis purpurea--pelodictyon luteolum--photobacteria--carbon--fresh water--methane--sulfate--sulfur--article--bacterial phenomena and functions--bacterium--comparative study--isolation and purification--metabolism--microbiology--oxidation reduction reaction--photosynthesis--russian federation--season--species difference--bacteria--bacterial physiology--carbon isotopes--fresh water--methane--oxidation-reduction--photosynthesis--seasons--siberia--species specificity--sulfates--sulfur isotopes--water microbiology
Аннотация: Microbiological and isotopic-geochemical investigations of the brackish meromictic lakes Shira and Shunet were performed in the steppe region of Khakasia in winter. Measurements made with a submersed sensor demonstrated that one-meter ice transmits light in a quantity sufficient for oxygenic and anoxygenic photosynthesis. As in the summer season, in the community of phototrophic bacteria found in Lake Shira, the purple sulfur bacteria Amoebobacter purpureus dominated, whereas, in Lake Shunet, the green sulfur bacteria Pelodictyon luteolum were predominant. Photosynthetic production, measured using the radioisotopic method, was several times lower than that in summer. The rates of sulfate reduction and production and oxidation of methane in the water column and bottom sediments were also lower than those recorded in summer. The process of anaerobic methane oxidation in the sediments was an exception, being more intense in winter than in summer. The data from radioisotopic measurements of the rates of microbial processes correlate well with the results of determination of the isotopic composition of organic and mineral carbon (? 13C) and hydrogen sulfide and sulfate (? 34S) and suggest considerable seasonal variations in the activity of the microbial community in the water bodies investigated.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N.O., Kalacheva G.S., Volova T.G., Degermendzhi A.G.
Заглавие : Structure of hydrocarbons synthesized by the alga Botryococcus isolated from Lake Shira.
Место публикации : Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2001. - Vol. 378. - С. 265-269. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--hydrocarbon--sea water--article--chemical structure--chemistry--classification--green alga--isolation and purification--metabolism--microbiology--russian federation--species difference--algae, green--fatty acids--hydrocarbons--molecular structure--seawater--siberia--species specificity
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trusova M.Yu., Gladyshev M.I.
Заглавие : Species composition of winter bacterioplankton in blooming and nonblooming reservoirs as determined by 16S rRNA sequences
Место публикации : Doklady Biological Sciences. - 2005. - Vol. 405, Is. 1-6. - С. 443-445. - ISSN 00124966 (ISSN) , DOI 10.1007/s10630-005-0160-4
Ключевые слова (''Своб.индексиров.''): fresh water--rna 16s--article--bacterium--classification--genetic variability--genetics--isolation and purification--microbiology--molecular genetics--phylogeny--plankton--russian federation--season--species difference--bacteria--fresh water--molecular sequence data--phylogeny--plankton--rna, ribosomal, 16s--seasons--siberia--species specificity--variation (genetics)--water microbiology
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tolomeyev A.P., Zadereev Y.S.
Заглавие : An in situ method for the investigation of vertical distributions of zooplankton in lakes: Test of a two-compartment enclosure
Место публикации : Aquatic Ecology. - 2005. - Vol. 39, Is. 1-2. - С. 181-188. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-004-5732-0
Ключевые слова (''Своб.индексиров.''): anoxic hypolimnion--solar radiation--stratified lake--vertical migration--zooplankton--biotic factor--enclosure experiment--lake ecosystem--physicochemical property--vertical distribution--zooplankton--animalia--arctodiaptomus salinus--brachionus plicatilis
Аннотация: Two-section enclosures were designed for the investigation of the effect of various physicochemical and biological factors on vertical distribution of zooplankton in situ. The framework of the enclosure was a cylindrical polyethylene column without any partitions inside, in which the isolation of animals in different sections after in situ exposure was achieved by pinching the flexible central part of the column. Enclosures were tested at the brackish stratified meromictic Lake Shira (Russia, Khakasia). The absence of fish and carnivorous zooplankton in the lake suggests that the vertical distribution of zooplankton is mainly determined by physicochemical gradients in the water column. Experiments and field observations demonstrated that all age and size groups of Arctodiaptomus salinus and Brachionus plicatilis strongly avoided surface layers during the daylight. The escape of zooplankton from the anoxic hypolimnion was less active. Statistically significant avoidance was observed only for copepodites C4-C5 and females of A. salinus. The relatively simple construction of the columns and easy handling during the experiment were the factors that favoured the use of this device to perform in situ basic tests of the effect of different factors on the vertical distribution of zooplankton. В© Springer 2005.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Illarrionov B.A., Blinov V.M., Douchenko A.P., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I.
Заглавие : Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes
Место публикации : Gene. - 1990. - Vol. 86, Is. 1. - С. 89-94. - ISSN 03781119 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--expression in e. coli--luciferase--molecular evolution--nucleotide sequence--protein alignment--recombinant dna--luciferase--amino acid sequence--article--bioluminescence--fungus--gene structure--genetic engineering--heredity--nonhuman--nucleotide sequence--priority journal--vibrionaceae--acyltransferases--amino acid sequence--bacterial proteins--base sequence--cloning, molecular--dna, bacterial--genes, structural, bacterial--luciferase--luminescence--molecular sequence data--operon--photobacterium--restriction mapping--escherichia coli--fungi--photobacterium leiognathi--vibrio harveyi--vibrionaceae
Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. В© 1990.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A, Pechurkin N.S., Sarangova A.B., Pisman T.I.
Заглавие : Effect of bacterial population density on germination wheat seeds and dynamics of simple artificial ecosystems
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1611-1615. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00257-5
Ключевые слова (''Своб.индексиров.''): bacteria--biomass--ecosystems--plants (botany)--seed--artificial soil--germination--photoassimilation--space research--artificial ecosystem--article--comparative study--ecosystem--germination--growth, development and aging--isolation and purification--microbiology--physiology--plant root--plant seed--pseudomonas fluorescens--pseudomonas putida--wheat--ecosystem--germination--plant roots--pseudomonas fluorescens--pseudomonas putida--seeds--triticum
Аннотация: Effect of the size of rhizospheric bacterial populations on germination of seeds and development of simple terrestrial "wheat plants - rhizospheric microorganisms - artificial soil" and "wheat plants - artificial soil" systems has been studied. Experiments demonstrated that within specify ranges in the inoculate, the rhizospheric bacteria are capable of increasing the yield of germinated seeds and stimulate the growth of plantlets. Germination of seeds inoculated with bacteria was either stimulated, or inhibited or remained at control levels depending on the amount of bacteria. Plant biomass growth and total photoassimilation has been found to depend on the amount of bacteria on the plant roots: the higher the amount of bacteria on plant roots, the smaller is the biomass of plants but the total photoassimilation is, higher. Thus, depending on the amount of bacteria on the roots of plants the system either increases the biomass of plants or increases the total photoassimilation, i.e. "pumps" carbon through itself involving bacteria. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Однотомное издание
Шифр издания : А.с. 1035962!-817652
Автор(ы) : Межевикин В.В., Высоцкий Е.С., Заворуев В.В., Родичева Э.К.
Заглавие : Способ получения препарата бактериальной люциферазы .-
Выходные данные : Б.м.,Б.г.
Коллективы : Ин-т биофиз. СО АН СССР
Цена : Б.ц.
ГРНТИ : 34.27.51
Предметные рубрики: ЛЮЦЕФЕРАЗА
ПОЛУЧЕНИЕ
СПОСОБ
PHOTOBACTERIUM LEIOGNATHI
БАКТЕРИИ
ENZYME ISOLATION
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S.E., Boyandin A., Lankin Y., Kotov D., Rodicheva E., Popova L.
Заглавие : BIOLUMBASE - The database of natural and transgenic bioluminescent organisms
Место публикации : Luminescence. - 2005. - Vol. 20, Is. 2. - С. 90-96. - ISSN 15227235 (ISSN) , DOI 10.1002/bio.809
Ключевые слова (''Своб.индексиров.''): bioluminescence--database--luminous bacteria--lux gene--marine--article--bacterial strain--bacterium culture--bacterium isolation--bioluminescence--data base--gene construct--medical information--transgenics--wide area network--bacteria--bacterial proteins--databases, factual--ecology--luminescence--luminescent proteins--marine biology--organisms, genetically modified--photobacterium--transgenes
Аннотация: The Institute of Biophysics SB RAS hosts and maintains a specialized collection of luminous bacteria (CCIBSO 836) containing over 700 strains isolated in various regions of the world's oceans. The culture collection is a source of lux genes and biologically active substances. The wide application of bioluminescence in medicine and ecology has given importance to analys-ing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as on their features that are closely interrelated with bioluminescence. The aims of our BIOLUMBASE database are: gathering information on microorganisms with lux genes, their analysis and free access, and distribution of this data throughout the global network. The database includes two sections, natural and transgenic luminous microorganisms, and is updated by our own experimental results, the published literature and internet resources. For the future, a publicly available internet site for BIOLUMBASE is planned. This will list the strains and provide comprehensive information on the properties and functions of luminous bacteria, the mechanisms of regulation of bioluminescence systems, constructs with lux genes, and applications of bioluminescence in microbiology, ecology, medicine and biotechnology. It is noteworthy that this database will also be useful for evaluation of biological hazards of transgenic strains. Users will be able to carry out bibliographic and strain searches starting from any feature of interest. Copyright В© 2005 John Wiley & Sons, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lobova T.I., Barkhatov Y.V., Salamatina O.V., Popova L.Yu.
Заглавие : Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of Lake Shira as an indicator of human impact on the ecosystem
Место публикации : Microbiological Research. - 2008. - Vol. 163, Is. 2. - С. 152-160. - ISSN 09445013 (ISSN) , DOI 10.1016/j.micres.2006.03.014
Ключевые слова (''Своб.индексиров.''): antibiotic resistance--aquatic ecosystems--heterotrophic bacteria--human impact--monitoring--antibiotics--bacteria--ecosystems--environmental impact--mammals--antibiotic resistance--aquatic ecosystems--heterotrophic bacteria--materials--antiinfective agent--fresh water--allochthony--anthropogenic effect--antibiotic resistance--bacterium--concentration (composition)--heterotrophy--intertidal environment--monitoring--recreational facility--spring (season)--summer--animal--article--bacterial count--bacterium--drug effect--ecosystem--environmental monitoring--heterotrophy--human--isolation and purification--methodology--microbiological examination--microbiology--multidrug resistance--russian federation--season--animals--anti-bacterial agents--bacteria--colony count, microbial--drug resistance, multiple, bacterial--ecosystem--environmental monitoring--fresh water--heterotrophic processes--humans--microbial sensitivity tests--russia--seasons--eurasia--khakassia--lake shira--russian federation--animalia--bacteria (microorganisms)
Аннотация: Resistance to Ampicillin and Kanamycin displayed by heterotrophic bacteria isolated in Summer and in Spring from the littoral and the central parts of Lake Shira (a therapeutic lake in the Khakasia Republic, Russia) has been investigated. It has been found that in Summer, human and animal microflora featuring multiple antibiotic resistance (to Ampicillin and Kanamycin) predominates in all the studied stations of the littoral zone of the lake. In Spring, concentrations of bacteria featuring multiple antibiotic resistance decrease significantly and bacteria sensitive to antibiotics predominate in the lake. Emergence of multiple antibiotic resistance in bacteria of Lake Shira is caused by the input of allochthonous bacteria into the lake; this feature of heterotrophic bacteria of Lake Shira can be used to monitor the impact on the ecosystem made by health resorts. В© 2006 Elsevier GmbH. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A., Borisova V.V., Vereshchagina T.A., Fomenko E.V., Anshits A.G., Gitelson I.I.
Заглавие : Affine magnetic sorbents supported on coal ash microspheres for recombinant protein isolation
Место публикации : Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 2. - С. 215-220. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683809020173
Ключевые слова (''Своб.индексиров.''): clytia gregaria--obelia longissima
Аннотация: The results of the development and utilization of an affine magnetic sorbent with Ni2+ ions immobilized on coal ash microspheres are reported. The applicability of the material in the isolation of Histag proteins is demonstrated by examples of the recombinant green fluorescent protein from Clytia gregaria and the Ca2+ regulated photoprotein obelin from Obelia longissima. The specific sorption capacity of the sorbent was 2-7 mg/cm3 for medium-size proteins (20-30 kDa). The particles are suitable for chromatography with the presence of chaotropic agents and EDTA. They are easy to manipulate as isolation of a target protein takes 30-35 min. On the one hand, the elevated affinity of the sorbent to proteins rich in native histidines may result in a high degree of irreversible sorption; on the other hand, it allows isolation of such proteins without the introduction of artificial polyhistidine fragments. В© 2009 Pleiades Publishing, Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : ILLARIONOV B.A., MARKOVA S.V., BONDAR V.S., VYSOTSKY E.S., GITELSON J.I.
Заглавие : ISOLATION AND EXPRESSION OF CDNA CODING FOR PHOTOPROTEIN OBELIN FROM HYDROID OBELIA-LONGISSIMA
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1992. - Vol. 326, Is. 5. - С. 911-913. - 3. - ISSN 0869-5652
Примечания : Cited References: 12
Предметные рубрики: AEQUORIN
PROTEIN
PHIALIDIN
CLONING
CA-2+
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : VYSOTSKII Y.S., BONDAR V.S., GITELZON I.I.
Заглавие : ISOLATION AND PROPERTIES OF VARIOUS MOLECULAR-FORMS OF CA2+-ACTIVATED PHOTOPROTEIN OBELIN
Колич.характеристики :4 с
Место публикации : DOKLADY AKADEMII NAUK SSSR: MEZHDUNARODNAYA KNIGA, 1991. - Vol. 321, Is. 1. - С. 214-217. - ISSN 0002-3264
Примечания : Cited References: 14
Предметные рубрики: CALCIUM-ACTIVATED PHOTOPROTEINS
CTENOPHORES MNEMIOPSIS SP
BEROE-OVATA
AEQUORIN
PROTEIN
PURIFICATION
EXTRACTION
PHIALIDIN
SEQUENCE
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Vysotsky E.S., Zavoruev V.V., Mezhevikin V.V., Raibekas A.A.
Заглавие : Obtaining bacterial luciferase for bioluminescent analysis
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1988. - Vol. 24, Is. 6. - С. 745-753. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--enzyme isolation--enzyme subunit structure--molecular weight--nonhuman--photobacterium leiognathi
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : ILLARIONOV B.A., MARKOVA S.V., BONDAR V.S., VYSOTSKY E.S., GITELSON J.I.
Заглавие : ISOLATION AND EXPRESSION OF CDNA CODING FOR PHOTOPROTEIN OBELIN FROM HYDROID OBELIA-LONGISSIMA
Колич.характеристики :3 с
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1992. - Vol. 326, Is. 5. - С. 911-913. - ISSN 0869-5652
Примечания : Cited References: 12
Предметные рубрики: AEQUORIN
PROTEIN
PHIALIDIN
CLONING
CA-2+
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S., Bondar' V.S.
Заглавие : Study of the luminescence system of the soil enchytraeid Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady Biochemistry and Biophysics. - 2003. - Vol. 391, Is. 1-6. - С. 204-207. - ISSN 16076729 (ISSN) , DOI 10.1023/A:1025105323648
Ключевые слова (''Своб.индексиров.''): adsorption chromatography--animal cell--annelid worm--article--luminescence--nonhuman--purification--animal--drug antagonism--isolation and purification--metabolism--physiology--soil--animalia--annelida--clitellata--enchytraeidae--edetic acid--luciferase--magnesium--animals--edetic acid--luciferases--luminescent measurements--magnesium--oligochaeta--soil
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates
Место публикации : Biochemistry. - 1995. - Vol. 34, Is. 10. - С. 3300-3309. - ISSN 00062960 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--recombinant protein--article--ligand binding--nonhuman--priority journal--protein isolation--protein protein interaction--protein stability--vibrionaceae--bacterial proteins--binding sites--carrier proteins--circular dichroism--flavin mononucleotide--fluorescence polarization--genes, bacterial--kinetics--ligands--luciferase--luminescence--molecular sequence data--photobacterium--recombinant proteins--spectrophotometry--support, u.s. gov't, p.h.s.--photobacterium leiognathi--vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Raibekas A.A., Petushkov V.N.
Заглавие : Isolation and comparative study of two fluorescent flavoproteins of the luminous bacteria Photobacterium leiognathi
Место публикации : Biophysics. - 1990. - Vol. 35, Is. 2. - С. 377-379. - ISSN 00063509 (ISSN)
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Raibekas A.A., Petushkov V.N.
Заглавие : Isolation and comparative studies of two fluorescent flavoproteins of luminous bacteria Photobacterium leiognathi
Место публикации : Biofizika. - 1990. - Vol. 35, Is. 2. - С. 368-370. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): flavoprotein--fluorescence--letter--nonhuman--photobacterium leiognathi
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Puzyr' A.P., Purtov K.V., Petunin A.I., Burov A.E., Rodicheva E.K., Medvedeva S.E., Shpak B.A., Tyaglik A.B., Shimomura O..., Gitel'zon I.I.
Заглавие : Isolation of luminescence system from the luminescent fungus Neonothopanus nambi
Колич.характеристики :3 с
Коллективы : Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" [11, G34.31.0058]; Siberian Branch of the Russian Academy of Sciences [71]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2014. - Vol. 455, Is. 1. - С. 56-58. - ISSN 1607-6729, DOI 10.1134/S1607672914020045. - ISSN 1608-3091
Примечания : Cited References: 10. - This study was supported by the Program of the Government of the Russian Federation "On Measures to Attract the Leading Scientists to the Educational Institutions of Russia" (project no. 11, G34.31.0058) and the Siberian Branch of the Russian Academy of Sciences (project no. 71).
WOS
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20.