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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Zhila, Natalia, Kiselev, Evgeniy, Prudnikova, Svetlana, Vinogradova, Olga, Nikolaeva, Elena, Shumilova, Anna, Shershneva, Anna, Shishatskaya, Ekaterina
Заглавие : Poly(3-hydroxybutyrate)/metribuzin formulations: characterization, controlled release properties, herbicidal activity, and effect on soil microorganisms
Колич.характеристики :15 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2016. - Vol. 23, Is. 23. - С. 23936-23950. - ISSN 0944-1344, DOI 10.1007/s11356-016-7636-7. - ISSN 1614-7499(eISSN)
Примечания : Cited References:41. - This study was supported by the Russian Science Foundation (grant no. 14-26-00039).
Предметные рубрики: METRIBUZIN RELEASE
POLYHYDROXYALKANOATES
POLYMER
MATRIX
PESTICIDES
Ключевые слова (''Своб.индексиров.''): metribuzin--degradable poly-3-hydroxybutyrate--slow-release p(3hb)/met--formulations--release kinetics--agrostis stolonifera--setaria--macrocheata
Аннотация: Slow-release formulations of the herbicide metribuzin (MET) embedded in the polymer matrix of degradable poly-3-hydroxybutyrate [P(3HB)] in the form of microparticles, films, microgranules, and pellets were developed and tested. The kinetics of polymer degradation, MET release, and accumulation in soil were studied in laboratory soil microecosystems with higher plants. The study shows that MET release can be controlled by using different techniques of constructing formulations and by varying MET loading. MET accumulation in soil occurs gradually, as the polymer is degraded. The average P(3HB) degradation rates were determined by the geometry of the formulation, reaching 0.17, 0.12, 0.04, and 0.05 mg/day after 60 days for microparticles, films, microgranules, and pellets, respectively. The herbicidal activities of P(3HB)/MET formulations and commercial formulation Sencor Ultra were tested on the Agrostis stolonifera and Setaria macrocheata plants. The parameters used to evaluate the herbicidal activity were plant density and the weight of fresh green biomass measured at days 10, 20, and 30 after sowing. All P(3HB)/MET formulations had pronounced herbicidal activity, which varied depending on MET loading and the stage of the experiment. In the early phases of the experiment, the herbicidal effect of P(3HB)/MET formulations with the lowest MET loading (10 %) was comparable with that of the commercial formulation. The herbicidal effect of P(3HB)/MET formulations with higher MET loadings (25 and 50 %) at later stages of the experiment were stronger than the effect of Sencor Ultra.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Bondar V. S.
Заглавие : Biochemical changes causes lack of bioluminescence in fruiting bodies of Armillaria
Место публикации : Mycosphere: Guizhou Key Laboratory of Agricultural Biotechnology, 2017. - Vol. 8, Is. 1. - С. 9-17. - ISSN 20777000 (ISSN) , DOI 10.5943/mycosphere/8/1/2
Ключевые слова (''Своб.индексиров.''): enzymes and substrate of luminescent reaction--kinetics of luminescence--luminous mycelia--nonluminous fruiting bodies of fungus
Аннотация: Mycelium of Armillaria species exhibit bioluminescence in nature and when cultivated on artificial nutrient media. However, fruiting bodies do not emit visible light. The present study investigates biochemical changes which cause this phenomenon. Light emission was studied in experiments with mixtures of cold and hot extracts of the luminous mycelium of Armillaria borealis IBSO 2328 and nonluminous fruiting bodies of this fungus and an unidentified species of the genus (Armillaria sp.). Hot extracts of fruiting bodies of the nonluminous Pholiota squarrosa were used as the substrate analog of the luminescent reaction, as previously this fungus had been found to contain a high amount of this substance. Control experiments showed that cold extracts of A. borealis IBSO 2328 mycelium contained enzymes for the luminescent reaction, which is initiated after addition hot extracts of P. squarrosa fruiting bodies. Parallel experiments with extracts of the fruiting bodies of Armillaria showed that: (i) - cold extracts did not contain enzymes of the luminescent reaction or contain very small amounts of these enzymes and (ii) - hot extracts did not contain substrate of the luminescent reaction. Thus, the reason why fruiting bodies of Armillaria do not emit light is that they do not contain components required for visible luminescence. The study discusses possible causes why the enzymes and substrate of the luminescent reaction are not synthesized in fruiting bodies of Armillaria. © Guizhou Academy of Agricultural Sciences.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Deeva, Anna A., Temlyakova, Evgenia A., Sorokin, Anatoly A., Nemtseva, Elena V., Kratasyuk, Valentina A.
Заглавие : Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis
Колич.характеристики :5 с
Коллективы : RFBR [16-34-00746 mol_a]; Ministry of Education and Science of the Russian Federation [1762]; state budget allocated to the fundamental research at Russian Academy of Sciences [01201351504]
Место публикации : Bioinformatics: OXFORD UNIV PRESS, 2016. - Vol. 32, Is. 20. - С. 3053-3057. - ISSN 1367-4803, DOI 10.1093/bioinformatics/btw386. - ISSN 1460-2059(eISSN)
Примечания : Cited References:31. - The reported study was partially funded by RFBR according to the research project No. 16-34-00746 mol_a; by the Ministry of Education and Science of the Russian Federation [project No 1762] and by the state budget allocated to the fundamental research at the Russian Academy of Sciences [project No 01201351504].
Аннотация: Motivation: Bacterial luciferases are heterodimeric enzymes that catalyze a chemical reaction, so called bioluminescence, which causes light emission in bacteria. Bioluminescence is vastly used as a reporter system in research tools and commercial developments. However, the details of the mechanisms that stabilize and transform the reaction intermediates as well as differences in the enzymatic kinetics amongst different bacterial luciferases remain to be elucidated. Results: Amino acid sequences alignments for 21 bacterial luciferases (both alpha- and beta-subunits) were analyzed. For alpha-subunit, containing the enzyme active center, 48 polymorphic amino acid positions were identified. According to them, the sequences fell into two distinct groups known as slow and fast based on the decay rate of the bioluminescence reaction. The differences in the enzyme active site induced by structural polymorphism are analyzed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Bondar V. S.
Заглавие : The use of glowing wood as a source of luminescent culture of fungus mycelium
Колич.характеристики :17 с
Коллективы : RF Government [11.G34.31.0058]; SB RAS [71]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2016. - Vol. 7, Is. 1. - С. 1-17. - ISSN 2077-7000, DOI 10.5943/mycosphere/7/1/1
Примечания : Cited References:22. - The authors are grateful to Prof. A. Frank, Director of North Borneo Biostation, for the opportunity to carry out studies of glowing wood; to Nadezhda N. Kudashova, a senior researcher at the Institute of Biology and Biophysics at the Tomsk University, for identifying the species of nonluminous fungi. This study was supported by grant no. 11.G34.31.0058 (RF Government) and Projects no. 71 (SB RAS).
Предметные рубрики: BIOLUMINESCENCE CHARACTERISTICS
NEONOTHOPANUS-NAMBI
LIGHT-EMISSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--culture of luminous mycelia--kinetics of luminescent--reaction--light emitting wood--luminous fungus
Аннотация: In studies of fungal bioluminescence, not only fruiting bodies and spores of the fungus, but also samples of luminescent wood, leaf litter or soil may need to be used to derive pure mycelial culture. This study describes an approach to isolating the culture of luminescent fungal mycelium from samples of light-emitting wood found on Borneo Island in November-December 2013. A GelDoc XR Imaging System (Bio-Rad Laboratories, Inc., U.S.) was used for the first time to monitor luminescence and select luminous samples. This study shows that for successful isolation of the culture of luminescent mycelium out of the luminescent wood found in the forest, it is imperative to keep the samples moist (mycelium alive until there is water), while immediate and aseptic delivery of the samples to the laboratory is not a crucial condition (inner layers of wood is "sterile"). Investigation of the growth features of the isolated mycelium in various growing conditions revealed some peculiar properties of its luminescence in comparison with the known luminescent cultures of basidiomycetes. When grown on solid nutrient media, mycelium exhibits low growth rates, long-lasting luminescence (140 days or longer), and emergence and disappearance of local zones with high levels of light emission. Mycelium produced in submerged culture does not emit light, and this effect must be caused by the absence or a very low level of the luminescent reaction substrate in the biomass. The luminescence system isolated from mycelial biomass did not induce luminescent reaction in vitro upon the addition of NADPH (recording intensity is 60 100 URL/sec). We found that enzymes of the luminescence systems isolated from mycelium pellets retained their activity and catalyzed luminescent reaction when a hot extract of the luminous fungus Armillaria sp. (IBSO 2360) was added (near 1900 URL/sec). The same effect was obtained after addition of hot extracts from the fruiting bodies of nonluminous higher fungi Pholiota squarrosa, Cortinarius sp., Hypholoma capnoides and Chroogomphus rutilus (near 3500 URL/sec). The pure culture of luminescent mycelium has been registered in the Culture Collection of IBP SB RAS as IBSO 2371; now it can be used for various in vivo and in vitro studies, including identification of the fungus.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Zhila N. O., Kiselev E. G., Volova T. G.
Заглавие : Constructing Slow-Release Formulations of Metribuzin Based on Degradable Poly(3-hydroxybutyrate)
Место публикации : J. Agric. Food Chem. - 2016. - Vol. 64, Is. 28. - С. 5625-5632. - ISSN 00218561 (ISSN) , DOI 10.1021/acs.jafc.5b05896
Ключевые слова (''Своб.индексиров.''): controlled release--degradable poly(3-hydroxybutyrate)--herbicide--metribuzin--release kinetics--polyethylene glycols--weed control--controlled release--environmental release--herbicide release--laboratory system--matrix formulation--metribuzin--poly-3-hydroxybutyrate--release kinetics--herbicides
Аннотация: Experimental formulations of herbicide metribuzin embedded in matrices of degradable natural polymer poly(3-hydroxybutyrate) (P3HB) and its composites with poly(ethylene glycol) (PEG), poly-?-caprolactone (PCL), and wood powder have been prepared in the form of pressed pellets containing 75% polymeric basis (pure P3HB or its composite with a second component at a ratio of 7:3) and 25% metribuzin. Incubation of formulations in soil laboratory systems led to the degradation of the matrix and herbicide release. The most active release of metribuzin (about 60% of the embedded herbicide over 35 days) was detected for the P3HB/PEG carrier compared to the P3HB, P3HB/wood, and P3HB/PCL forms (30-40%). Thus, the study shows that herbicide release can be controlled by the matrix formulation. Metribuzin formulations exerted a significant herbicidal effect on the plant Agrostis stolonifera, used as a weed plant model. Application of these long-term formulations will make it possible to reduce environmental release of chemicals, which will restrict the rate of their accumulation in trophic chains of ecosystems and abate their adverse effects on the biosphere. © 2016 American Chemical Society.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova, Marina D., Markova, Svetlana V., Vysotski, Eugene S.
Заглавие : Tyr72 and Tyr80 are Involved in the Formation of an Active Site of a Luciferase of Copepod Metridia longa
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [14-14-01119]
Место публикации : Photochem. Photobiol.: WILEY, 2017. - Vol. 93, Is. 2. - С. 503-510. - ISSN 0031-8655, DOI 10.1111/php.12694. - ISSN 1751-1097(eISSN)
Примечания : Cited References:41. - This work was supported by the grant 14-14-01119 of the Russian Science Foundation.
Предметные рубрики: CA2+-REGULATED PHOTOPROTEIN OBELIN
COELENTERAZINE-BINDING PROTEIN
Аннотация: Luciferase of copepod Metridia longa (MLuc) is a naturally secreted enzyme catalyzing the oxidative decarboxylation of coelenterazine with the emission of light. To date, three nonallelic isoforms of different lengths (17-24 kDa) for M. longa luciferase have been cloned. All the isoforms are single-chain proteins consisting of a 17-residue signal peptide for secretion, variable N-terminal part and conservative C-terminus responsible for luciferase activity. In contrast to other bioluminescent proteins containing a lot of aromatic residues which are frequently involved in light emission reaction, the C-terminal part of MLuc contains only four Phe, two Tyr, one Trp and two His residues. To figure out whether Tyr residues influence bioluminescence, we constructed the mutants with substitution of Tyr to Phe (Y72F and Y80F). Tyrosine substitutions do not eliminate the ability of luciferase to bioluminescence albeit significantly reduce relative specific activity and change bioluminescence kinetics. In addition, the Tyr replacements have no effect on bioluminescence spectrum, thereby indicating that tyrosines are not involved in the emitter formation. However, as it was found that the intrinsic fluorescence caused by Tyr residues is quenched by a reaction substrate, coelenterazine, in concentration-dependent manner, we infer that both tyrosine residues are located in the luciferase substrate-binding cavity.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Kazantseva E. A., Varygina D. E., Volova T. G.
Заглавие : Constructing Slow-Release Formulations of Ammonium Nitrate Fertilizer Based on Degradable Poly(3-hydroxybutyrate)
Место публикации : J. Agric. Food Chem.: American Chemical Society, 2017. - Vol. 65, Is. 32. - С. 6745-6752. - ISSN 00218561 (ISSN) , DOI 10.1021/acs.jafc.7b01217
Ключевые слова (''Своб.индексиров.''): ammonium nitrate--degradable poly-3-hydroxybutyrate--embedding--fillers--nitrogen fertilizers--tablets--chemical contamination--ecology--ecosystems--fertilizers--fillers--nitrates--plastic coatings--ammonium nitrate--ammonium nitrate fertilizers--embedding--in-laboratory experiments--poly-3-hydroxybutyrate--slow release fertilizers--tablets--wheat (triticum aestivum l.)--nitrogen fertilizers
Аннотация: The present study describes construction and investigation of experimental formulations of ammonium nitrate embedded in a matrix of degradable natural polymer poly-3-hydroxybutyrate [P(3HB)] and P(3HB) blended with wood flour shaped as tablets, some of them coated with P(3HB). Kinetics of ammonium release into soil as dependent on the composition of the polymer matrix was investigated in laboratory experiments. The rates of fertilizer release from formulations coated with a biopolymer layer were considerably (two months or longer) slower than the rates of fertilizer release from uncoated formulations, while release from polymer and composite (polymer/wood flour) formulations occurred with comparable rates. The use of the experimental formulations in laboratory ecosystems with wheat (Triticum aestivum L.) was more effective than application of free ammonium nitrate. The advantage of the slow-release fertilizer formulations is that they are buried in soil together with the seeds, and the fertilizer remains effective over the first three months of plant growth. The use of such slow-release formulations will reduce the amounts of chemicals released into the environment, which will curb their accumulation in food chains of ecosystems and mitigate their adverse effects on the biosphere. © 2017 American Chemical Society.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Artemenko K. S., Bondar V. S.
Заглавие : Luminescence of cold extracts from mycelium of luminous basidiomycetes during long-term storage
Место публикации : Curr. Res. Environ. Appl. Mycol. J. Fungal: Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection, 2017. - Vol. 7, Is. 3. - С. 227-235. - ISSN 22292225 (ISSN) , DOI 10.5943/cream/7/3/9
Ключевые слова (''Своб.индексиров.''): armillaria borealis--kinetics of luminescence--lyophilic preparations--mycena citricolor--neonothopanus nambi
Аннотация: Cold extracts with high activities of enzymes of luminescent reaction were prepared from mycelia of luminous fungi Armillaria borealis IBSO 2328, Mycena citricolor IBSO 2331, and Neonothopanus nambi IBSO 2391. The authors describe techniques of preparing cold extracts with high levels of luminescence from mycelial biomass of different species of luminous basidiomycetes. The investigation of cold extracts showed that in experiments with freezing and thawing of the samples as well as in experiments with lyophilization followed by dissolution of the dry samples, the levels of enzyme activity were high, with in vitro luminescence exhibited after addition of NADPH and the hot extract containing the substrate. High activity levels of the enzymes of luminescent reaction were measured in lyophilized cold extracts stored over three years. In lyophilized preparations, the enzymes of luminescent reaction had high thermostability, even when dry preparations of cold extracts were exposed to a temperature of 100°C for 60 minutes. © Beijing Academy of Agriculture and Forestry Sciences.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E. V., Vysotski E. S.
Заглавие : Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin
Место публикации : J. Photochem. Photobiol. B Biol.: Elsevier B.V., 2017. - Vol. 174. - С. 97-105. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2017.07.021
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenteramide--coelenterazine--cysteine--photoprotein--serine
Аннотация: Bioluminescence of a variety of marine coelenterates is determined by Ca2+-regulated photoproteins. A strong interest in these proteins is for their wide analytical potential as intracellular calcium indicators and labels for in vitro binding assays. The presently known hydromedusan Ca2+-regulated photoproteins contain three (aequorin and clytin) or five (obelin and mitrocomin) cysteine residues with one of them strictly conserved. We have constructed Cys-free aequorin and obelin by substitution of all cysteines to serine residues. Such mutants should be of interest for researchers by the possibility to avoid the incubation with dithiothreitol (or ?-mercaptoethanol) required for producing an active photoprotein that is important for some prospective analytical assays in which the photoprotein is genetically fused with a target protein sensitive to the reducing agents. Cys-free mutants were expressed in Escherichia coli, purified, and characterized regarding the efficiency of photoprotein complex formation, functional activity, and conformational stability. The replacement of cysteine residues has been demonstrated to affect different properties of aequorin and obelin. Cys-free aequorin displays a two-fold lower specific bioluminescence activity but preserves similar activation properties and light emission kinetics compared to the wild-type aequorin. In contrast, Cys-free obelin retains only ~ 10% of the bioluminescence activity of wild-type obelin as well as binding coelenterazine and forming active photoprotein much less effectively. In addition, the substitution of Cys residues drastically changes the bioluminescence kinetics of obelin completely eliminating a “fast” component from the light signal decay curve. At the same time, the replacement of Cys residues increases conformational flexibility of both aequorin and obelin molecules, but again, the effect is more prominent in the case of obelin. The values of thermal midpoints of unfolding (Tm) were determined to be 53.3 ± 0.2 and 44.6 ± 0.4 °C for aequorin and Cys-free aequorin, and 49.1 ± 0.1 and 28.8 ± 0.3 °C for obelin and Cys-free obelin, respectively. Thus, so far only Cys-free aequorin is suitable as a partner for fusing with a tag sensitive to reducing agents since the aequorin mutant preserves almost 50% of the bioluminescent activity and can be produced with a substantial yield. © 2017 Elsevier B.V.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva, Elena V., Bartsev, Sergey I., van Berkel, Willem J. H., Vysotski, Eugene S.
Заглавие : Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca2+-regulated Photoproteins of Different Organisms
Колич.характеристики :8 с
Коллективы : RFBR [14-04-31092]; Russian Academy of Sciences [01201351504, 01201351502]
Место публикации : Photochem. Photobiol.: WILEY, 2017. - Vol. 93, Is. 2. - С. 495-502. - ISSN 0031-8655, DOI 10.1111/php.12664. - ISSN 1751-1097(eISSN)
Примечания : Cited References:55. - This work was supported by RFBR grant 14-04-31092 and the state budget allocated to the fundamental research at the Russian Academy of Sciences (projects 01201351504 and 01201351502).
Предметные рубрики: GREEN-FLUORESCENT PROTEIN
AEQUORIN BIOLUMINESCENCE
SEQUENCE-ANALYSIS
Аннотация: Upon binding their metal ion cofactors, Ca2+-regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca2+-regulated photoproteinsaequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculatademonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca2+-regulated photoproteins.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rozhko T. V., Guseynov O. A., Guseynova V. E., Bondar A. A., Devyatlovskaya A. N., Kudryasheva N. S.
Заглавие : Is bacterial luminescence response to low-dose radiation associated with mutagenicity?
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2017. - Vol. 177. - С. 261-265. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2017.07.010
Ключевые слова (''Своб.индексиров.''): bioassay--dna--low-dose radiation--luminous marine bacteria--mutations--bacteria--bioassay--bioluminescence--chemical activation--dna--dna sequences--genes--ionizing radiation--kinetics--luminescence--nucleic acids--phosphorescence--physiological models--radioisotopes--bacterial suspensions--beta-emitting radionuclides--low dose radiation--luminescence intensity--marine bacterium--mutations--photobacterium phosphoreum--physiological parameters--radiation--bacteria (microorganisms)--photobacterium phosphoreum
Аннотация: Luminous marine bacteria are widely used in bioassays with luminescence intensity being a physiological parameter tested. The purpose of the study was to determine whether bacterial genetic alteration is responsible for bioluminescence kinetics change under low-dose radiation exposure. The alpha-emitting radionuclide 241Am and beta-emitting radionuclide 3H were used as the sources of low-dose ionizing radiation. Changes of bioluminescence kinetics of Photobacterium phosphoreum in solutions of 241Am(NO3)3, 7 kBq/L, and tritiated water, 100 MBq/L, were studied; bioluminescence kinetics stages (absence of effect, activation, and inhibition) were determined. Bacterial suspension was sampled at different stages of the bioluminescent kinetics; the doses accumulated by the samples were close or a little higher than a tentative limit of a low-dose interval: 0.10 and 0.85 Gy for 241Am, or 0.11 and 0.18 Gy for 3H. Sequence analysis of the 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose alpha and beta radiation in the bacterial samples. Previous results on bacterial DNA exposed to low-dose gamma radiation (0.25 Gy) were analyzed and compared to those for alpha and beta irradiation. It is concluded that bioluminescence activation and/or inhibition under the applied conditions of low-dose alpha, beta and gamma radioactive exposure is not associated with DNA mutations in the gene sequences tested. © 2017 Elsevier Ltd
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rozhko T. V., Badun G. A., Razzhivina I. A., Guseynov O. A., Guseynova V. E., Kudryasheva N. S.
Заглавие : On the mechanism of biological activation by tritium
Место публикации : J. Environ. Radioact. - 2016. - Vol. 157. - С. 131-135. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2016.03.017
Ключевые слова (''Своб.индексиров.''): dna mutations--low-dose effect--luminous marine bacteria--radiation hormesis--tritium
Аннотация: The mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface (tritium-labeled films, 0.11, 0.28, 0.91, and 2.36 MBq/cm2). When using the tritium-labeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescence kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media. © 2016 Elsevier Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kobzeva T.V., Melnikov A.R., Karogodina T.Y., Zikirin S.B., Stass D.V., Molin Y.N., Rodicheva E.K., Medvedeva S.E., Puzyr A.P., Burov A.A., Bondar V.S., Gitelson J.I.
Заглавие : Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation
Колич.характеристики :8 с
Коллективы : Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29, Is. 7. - С. 703-710. - ISSN 1522-7235, DOI 10.1002/bio.2656. - ISSN 1522-7243
Примечания : Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058).
Предметные рубрики: BIOLUMINESCENCE
COMPONENTS
MECHANISMS
SYSTEM
Ключевые слова (''Своб.индексиров.''): higher luminous fungi--neonothopanus nambi--ionizing irradiation--reactive oxygen species--lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sukovataya I.E., Tyulkova N.A.
Заглавие : Kinetic analysis of bacterial water-organic media
Колич.характеристики :3 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2001. - Vol. 16: 11th International Bioluminescence and Chemiluminescence Symposium (SEP 06-10, 2000, PACIFIC GROVE, CALIFORNIA), Is. 4. - P271-273. - ISSN 1522-7235, DOI 10.1002/bio.649.abs
Примечания : Cited References: 10
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--organic solvents--michaelis constant
Аннотация: The interaction of luciferases from two types of luminous bacteria, Photobacterium leiognathi and Vibrio harveyi, with their substrates [the photorecovered. FMNH2 and long-chain aldehydes-decanal (C-10), dodecanal (C-12) and tetradecanal (C-14)] in water-organic media was analysed using kinetic graphical methods. Moderate concentrations of organic solvents have been demonstrated to activate the bioluminescence, while higher concentrations inhibit it. The interactions of these effectors with luciferases show different types of kinetics, which depend on concentrations of solvents, kinds of enzymes and substrates. The apparent value of the Michaelis constant, K-m for C-14 of both luciferases and for C-10 of luciferase V. harveyi is enhanced with increasing concentration of the organic solvent, but K-m for C-12 and C-10 of luciferase P. leiognathi decreases. Obviously, at the specific binding of aldehydes with luciferases in the first case, hydrophobic interactions are realized, but in second, the electrostatic interactions are realized. The series of changes in parameters of bioluminescence reaction catalysed by different luciferases is obviously determined by their structural peculiarities. Copyright (C) 2001 John Wiley & Sons, Ltd.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Kudinova I.Y., Esimbekova E.N., Kratasyuk V.A., Stom D.I.
Заглавие : The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems
Колич.характеристики :8 с
Место публикации : Chemosphere: PERGAMON-ELSEVIER SCIENCE LTD, 1999. - Vol. 38, Is. 4. - P751-758. - ISSN 0045-6535, DOI 10.1016/S0045-6535(98)00218-5
Примечания : Cited References: 7
Аннотация: Kinetics of the triple bioluminescent enzyme system: alcohol dehydrogenase - NADH:FMN-oxidoreductase - luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD(+)/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring. (C) 1998 Elsevier Science Ltd. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PUKHOV K.I., MAKARSKAYA G.V., YAKHNINA Y.I., PUKHOVA Y.I.
Заглавие : CHEMILUMINESCENT ANALYSIS OF THE KINETICS OF REACTIVE OXYGEN SPECIES GENERATION BY WHOLE-BLOOD CELLS UNDER COMPENSATIVE EXFUSIONS
Колич.характеристики :5 с
Место публикации : DOKLADY AKADEMII NAUK SSSR: MEZHDUNARODNAYA KNIGA, 1991. - Vol. 316, Is. 1. - P247-251. - ISSN 0002-3264
Примечания : Cited References: 11
Предметные рубрики: HYDROGEN-PEROXIDE
NEUTROPHIL
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SANDALOVA T.P., TYULKOVA N.A.
Заглавие : INACTIVATION OF BACTERIAL LUCIFERASES BY N-ETHYLMALEIMIDE
Колич.характеристики :7 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1992. - Vol. 57, Is. 6. - P552-558. - ISSN 0006-2979
Примечания : Cited References: 21
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
REACTIVE SULFHYDRYL
PHOTOBACTERIUM-LEIOGNATHI
VIBRIO-HARVEYI
BIOLUMINESCENCE
SUBUNIT
REGION
GENE
Ключевые слова (''Своб.индексиров.''): luciferase--n-ethylmaleimide
Аннотация: The kinetics of inactivation of luciferases from four species of luminescent bacteria by the thiol reagent N-ethylmaleimide were investigated The dependencies of inactivation on ionic strength differed among the enzymes. Increasing the molarity of the buffer increased the rate of inactivation of all luciferases except that of Vibrio harveyi. Modification of Photobacterium phosphoreum luciferase decreased the maximal intensity of bioluminescence, whereas modification of Photobacterium leiognathi and Vibrio fischeri luciferases in high ionic strength buffers decreased the maximal intensity of bioluminescence and changed the luminescence decay rate constant. High ionic strength apparently alters the conformational states of the luciferases.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A.
Заглавие : Tolerance of LSS plant component to elevated temperatures
Колич.характеристики :6 с
Место публикации : Acta Astronaut.: PERGAMON-ELSEVIER SCIENCE LTD, 2002. - Vol. 50, Is. 12. - P759-764. - ISSN 0094-5765, DOI 10.1016/S0094-5765(02)00010-3
Примечания : Cited References: 10
Аннотация: Stability of LSS based on biological regeneration of water, air and food subject to damaging factors is largely dependent on the behavior of the photosynthesizing component represented, mainly, by higher plants. The purpose of this study is to evaluate the tolerance of uneven-aged wheat and radish cenoses to temperature effects different in time and value. Estimation of thermal tolerance of plants demonstrated that exposure for 20 h to the temperature increasing to 45degreesC brought about irreversible damage both in photosynthetic processes (up to 80% of initial value) and the processes of growth and development. Kinetics of visible photosynthesis during exposure to elevated temperatures can be used to evaluate critical exposure time within the range of which the damage of metabolic processes is reversible. With varying light intensity and air temperature it is possible to find a time period admissible for the plants to stay under adverse conditions without considerable damage of metabolic processes. (C) 2002 Elsevier Science Ltd. All rights reserved.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Syrvacheva D. A., Zhila N. O., Sukovatiy A. G.
Заглавие : Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoate monomer by Cupriavidus eutrophus B10646
Место публикации : J. Chem. Technol. Biotechnol. - 2016. - Vol. 91, Is. 2. - С. 416-425. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.4592
Ключевые слова (''Своб.индексиров.''): growth kinetics--physicochemical and mechanical properties--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain cupriavidus eutrophus b10646--biomaterials--biomechanics--chemical industry--cultivation--growth kinetics--mechanical properties--organic compounds--polymers--sodium--3-hydroxyhexanoate--bacterial strains--cultivation conditions--kinetic properties--physico-chemical and mechanical properties--physiological range--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain--strain
Аннотация: BACKGROUND: P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS: Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION: For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5-6gL-1) and high content of the polymer (60-75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. © 2014 Society of Chemical Industry © 2016 Society of Chemical Industry.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L. A.
Заглавие : Creation of artificial luciferases to expand their analytical potential
Место публикации : Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - С. 919-929. - ISSN 13862073 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioimaging--bioluminescence--luciferase--luciferase-based assay--luciferin--mutagenesis--photoprotein--reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescencebased analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc. © 2015 Bentham Science Publishers.
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