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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova S.V., Vysotski E.S.
Заглавие : Coelenterazine-dependent luciferases
Место публикации : Biochemistry Moscow. - 2015. - Vol. 80, Is. 6. - С. 714-732. - ISSN 00062979 (ISSN) , DOI 10.1134/S0006297915060073
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--luciferase--luciferin--coelenterata--cypridina luciferin--fungi--hexapoda--mollusca--protozoa
Аннотация: Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives of different taxa despite the same final result of this biochemical process. Among the several substrates of bioluminescent reactions identified in marine luminous organisms, the most commonly used are imidazopyrazinone derivatives such as coelenterazine and Cypridina luciferin. Although the substrate used is the same, bioluminescent proteins that catalyze light emitting reactions in taxonomically remote luminous organisms do not show similarity either in amino acid sequences or in spatial structures. In this review, we consider luciferases of various luminous organisms that use coelenterazine or Cypridina luciferin as a substrate, as well as modifications of these proteins that improve their physicochemical and bioluminescent properties and therefore their applicability in bioluminescence imaging in vivo. © 2015 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K.V., Petushkov V.N., Baranov M.S., Mineev K.S., Rodionova N.S., Kaskova Z.M., Tsarkova A.S., Petunin A.I., Bondar V.S., Rodicheva E.K., Medvedeva S.E., Oba Y., Oba Y., Arseniev A.S., Lukyanov S., Gitelson J.I., Yampolsky I.V.
Заглавие : The Chemical Basis of Fungal Bioluminescence
Место публикации : Angew. Chem. Int. Ed. - 2015. - Vol. 54, Is. 28. - С. 8124-8128. - ISSN 14337851 (ISSN) , DOI 10.1002/anie.201501779
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioorganic chemistry--biosynthesis--luciferin--natural products--biochemistry--bioluminescence--biosynthesis--metabolites--phosphorescence--biochemical mechanisms--bioorganic chemistry--luciferin--natural products--plant secondary metabolites--structural similarity--fungi
Аннотация: Many species of fungi naturally produce light, a phenomenon known as bioluminescence, however, the fungal substrates used in the chemical reactions that produce light have not been reported. We identified the fungal compound luciferin 3-hydroxyhispidin, which is biosynthesized by oxidation of the precursor hispidin, a known fungal and plant secondary metabolite. The fungal luciferin does not share structural similarity with the other eight known luciferins. Furthermore, it was shown that 3-hydroxyhispidin leads to bioluminescence in extracts from four diverse genera of luminous fungi, thus suggesting a common biochemical mechanism for fungal bioluminescence. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi, Maxim A., Kaskova, Zinaida M., Rodionova, Natalja S., Baranov, Mikhail S., Gorokhovatsky, Andrey Yu., Kotlobay, Alexey, Solntsev, Kyril M., Tsarkova, Aleksandra S., Petushkov, Valentin N., Yampolsky, Ilia V.
Заглавие : Novel Mechanism of Bioluminescence: Oxidative Decarboxylation of a Moiety Adjacent to the Light Emitter of Fridericia Luciferin
Колич.характеристики :3 с
Коллективы : Russian Science Foundation [14-50-00131], President of the Russian Federation; National Science Foundation [CHE-1213047]
Место публикации : Angew. Chem.-Int. Edit.: WILEY-V C H VERLAG GMBH, 2015. - Vol. 54, Is. 24. - С. 7065-7067. - ISSN 1433-7851, DOI 10.1002/anie.201501668. - ISSN 1521-3773(eISSN)
Примечания : Cited References:15. - We thank Dr. K.V. Antonov for acquisition of LC-HRMS spectra and Prof. Gary Schuster for fruitful discussion. This work was supported by the Russian Science Foundation grant 14-50-00131. M.S.B. acknowledges support by a stipend program of the President of the Russian Federation. K.M.S. acknowledges generous support from the National Science Foundation (CHE-1213047). This research was carried out using the equipment provided by IBCH core facility (CKP IBCH).
Предметные рубрики: STRUCTURE ELUCIDATION
CHEMILUMINESCENCE
CYPRIDINA
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioorganic chemistry--luciferin--oxidative--decarboxylation--peptides
Аннотация: A novel luciferin from a bioluminescent Siberian earthworm Fridericia heliota was recently described. In this study, the Fridericia oxyluciferin was isolated and its structure elucidated. The results provide insight into a novel bioluminescence mechanism in nature. Oxidative decarboxylation of a lysine fragment of the luciferin supplies energy for light generation, while a fluorescent CompX moiety remains intact and serves as the light emitter.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S. E., Artemenko K. S., Krivosheenko A. A., Rusinova A. G., Rodicheva E. K., Puzyr A. P., Bondar V. S.
Заглавие : Growth and light emission of luminous basidiomycetes cultivated on solid media and in submerged culture
Колич.характеристики :13 с
Коллективы : RF Government [11.G34.31.058]; SB RAS [71, 38]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2014. - Vol. 5, Is. 4. - С. 565-577. - ISSN 2077-7000, DOI 10.5943/mycosphere/5/4/9
Примечания : Cited References:23. - This study was supported by grant No. 11.G34.31.058 (RF Government) and Projects No. 71 and No. 38 (SB RAS).
Предметные рубрики: MYCELIAL GROWTH
PANELLUS-STYPTICUS
BIOLUMINESCENCE
LUMINESCENCE
Ключевые слова (''Своб.индексиров.''): luminescence--luminous higher fungi--mycelium
Аннотация: There are higher fungi that emit visible light; however, little is known about their requirements for good growth and bright luminescence. Knowledge of these requirements is extremely important for maintaining fungal cultures in laboratory conditions and preparation of luminous mycelia for research purposes. Luminous higher fungi Panellus stipticus, Armillaria sp. and Neonothopanus nambi isolated from different climatic areas and maintained in CCIBSO 836 (Collection of IBP SB RAS, Russia) were used for experiments. Techniques for static and submerged cultivation of mycelia of higher fungi have been developed and optimized for the production of samples of aerial and globular mycelia with prolonged and stable luminescence. We investigated the growth characteristics and luminescence of mycelia cultivated in/on different nutrient media, and the effects of deionized water and mechanical damage on the light emission of mycelia. An increase in luminescence intensity of fungal mycelia can be obtained during cultivation of fungi on a nutrient medium with a certain composition. A significant increase in light emission from N. nambi mycelium can also be obtained after its incubation in water and mechanical damage. The light emission from N. nambi mycelium was greatly enhanced after these treatments, in contrast to the mycelia of Armillaria sp. or P. stipticus. Cultivation conditions that enable growing mycelia with high levels of luminescence will expedite further studies to gain a better understanding of fungal bioluminescence.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid’ko A. F., Botvich I. Y., Pisman T. I., Shevyrnogov A. P.
Заглавие : Analysis of the polarization characteristics of wheat and maize crops using land-based remote-sensing measurements
Место публикации : Biophysics. - 2015. - Vol. 60, Is. 4. - С. 668-671. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350915040223
Ключевые слова (''Своб.индексиров.''): crops--optical remote sensing techniques--polarization characteristics
Аннотация: This paper presents an analysis of the study of the polarized component of the reflectance factor (Rq) and the degree of polarization (P) of wheat and maize crops depending on the wavelength. The polarization characteristics were obtained in the field from an elevated work platform at heights of 10 to 18 m in June and July. Measurements were performed using a double-beam spectrophotometer with a polarized light filter attachment within the spectral range from 400 to 820 nm. The viewing angle was no greater than 20° with respect to the nadir. The reflection spectra of wheat and maize crops that were obtained using a polarizer adjusted to transmit the maximum and minimum amounts of light (Rmax and Rmin) were studied. Based on these reflection spectra, the polarization characteristics, which differ in the visible and infrared spectral regions, were determined and analyzed. © 2015, Pleiades Publishing, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya, O. A., Ronzhin, N. O., Medvedeva, S. E., Bondar', V. S.
Заглавие : Total peroxidase and catalase activity of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in comparison with the level of light emission
Колич.характеристики :6 с
Коллективы : Siberian Branch of the Russian Academy of Sciences [71]
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 51, Is. 4. - С. 419-424. - ISSN 0003-6838, DOI 10.1134/S0003683815040110. - ISSN 1573-8183(eISSN)
Примечания : Cited References:35. - The authors are grateful to N. V. Psurtseva (curator of the collection of basidiomycetes of the Botanical Institute, Russian Academy of Science) for help with the species affiliation of the IBSO 2328 culture. This work was supported by the Program of Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences, project no. 71.
Предметные рубрики: OXIDATIVE STRESS
SYSTEM
FUNGI
BIOLUMINESCENCE
LUMINESCENCE
Ключевые слова (''Своб.индексиров.''): basidiomycetes--luminescence--peroxidase--catalase
Аннотация: The peroxidase and catalase activities in the mycelium of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in normal conditions and under stress were compared. An increase in the luminescence level was observed under stress, as well as an increase in peroxidase and catalase activities. Moreover, the peroxidase activity in extracts of A. borealis mycelium was found to be almost one and a half orders of magnitude lower, and the catalase activity more than two orders of magnitude higher in comparison with the N. nambi mycelium. It can be suggested that the difference between the brightly luminescent and dimly luminescent mycelium of N. nambi is due to the content of (HO2)-O-2 or other peroxide compounds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vanyukov, Viatcheslav V., Mikheev, Gennady M., Mogileva, Tatyana N., Puzyr, Alexey P., Bondar, Vladimir S., Svirko, Yuri P.
Заглавие : Near-IR nonlinear optical filter for optical communication window
Колич.характеристики :4 с
Коллективы : Academy of Finland [278252, 288547], Emil Aaltosen Saatio foundation, Russian Foundation for Basic Research [13-02-96016 r_ural_a], Erasmus staff exchange support [Dno 275/04.04.02.01], FP7 Marie Curie project FANCEE
Место публикации : Appl. Optics: OPTICAL SOC AMER, 2015. - Vol. 54, Is. 11. - С. 3290-3293. - ISSN 1559-128X, DOI 10.1364/AO.54.003290. - ISSN 2155-3165(eISSN)
Примечания : Cited References:21. - This work was supported by the Academy of Finland (grants no. 278252 and no. 288547), the Emil Aaltosen Saatio foundation, Russian Foundation for Basic Research (project no. 13-02-96016 r_ural_a), the Erasmus staff exchange support (Dno 275/04.04.02.01), and FP7 Marie Curie project FANCEE.
Предметные рубрики: DETONATION NANODIAMOND CLUSTERS
LIGHT-SCATTERING
SUSPENSIONS
Аннотация: We report on a high performance nonlinear optical filter for the telecommunication window that employs detonation nanodiamonds (NDs). The nanosecond Z-scan experiments revealed that the heavy water ND suspensions enable strong optical limiting in the wavelength range of 1400-1675 nm. We observed an enhancement of the optical limiting performance in the blue part of the communication window. In particular, at the wavelength of 1400 nm the transmittance of the 2 mm thick sample with 4.5 wt. % ND concentration is suppressed by 45% for the input fluence of 3.8 J/cm(2). The proposed nonlinear optical filter employs the phenomena of the nonlinear absorption and the nonlinear light scattering in ND suspensions. (C) 2015 Optical Society of America
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevsky N.A., Kolmakov V.I., Anishchenko O.V., Gorbaneva T.B.
Заглавие : Using DCMU-fluorescence method for the identification of dominant phytoplankton groups
Колич.характеристики :12 с
Место публикации : J. Appl. Phycol.: SPRINGER, 2005. - Vol. 17, Is. 6. - P483-494. - ISSN 0921-8971, DOI 10.1007/s10811-005-2903-x
Примечания : Cited References: 28
Предметные рубрики: IN-VIVO
CHLOROPHYLL FLUORESCENCE
FLUOROMETRIC METHOD
SITU
PHOTOSYNTHESIS
CYANOBACTERIA
ALGAE
EVOLUTION
CULTURES
LAKES
Ключевые слова (''Своб.индексиров.''): algae--chlorophyll--fluorescence--vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zavorueva E.N., Ushakova S.A.
Заглавие : Characteristics of slow induction curve of chlorophyll fluorescence and CO2 exchange for the assessment of plant heat tolerance at various levels of light intensity
Колич.характеристики :8 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2004. - Vol. 51, Is. 3. - P294-301. - ISSN 1021-4437, DOI 10.1023/B:RUPP.0000028674.39572.1c
Примечания : Cited References: 18
Ключевые слова (''Своб.индексиров.''): triticum aestivum--raphanus sativus var. minor--fluorescence--heat tolerance--pigments--co2 exchange
Аннотация: The heat tolerance of wheat (Triticum aestivum L.) and radish (Raphanus sativus L. var. minor) cenoses exposed to elevated and damaging air temperatures (35 degreesC for 20 h, 45 degreesC for 7 h) under photoculture conditions at various levels of photosynthetically active radiation (PAR) was assessed by measuring characteristics of the slow induction curve of chlorophyll fluorescence at 682 and 734 nm and the CO2 exchange rate. Irrespective of the illumination level, the exposure of the cenoses to 35 degreesC did not induce irreversible changes in the plant photosynthetic apparatus. The lowest extent of damage to wheat and radish cenoses exposed to 45 degreesC was observed at 150 W/m(2) of PAR, whereas the highest damage of the plants was observed at an illumination level that was close to the compensation point of the cenose photosynthesis (50-70 W/m(2) of PAR at air temperature of 24 degreesC). Viability index proved to be the most sensitive. characteristic, compared to other characteristics, which were determined by measuring the slow phase of fluorescence induction at 682 and 734 nm. In the cenoses studied, the pattern of changes in the viability index in response to a stress factor was close to the changes in the photosynthetic rate.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Karnachuk R.A., Vaishlya O.B., Dorofeev V.Y., Ushakova S.A., Tikhomirov A.A., Lasseur C..., Gros J.B.
Заглавие : Effect of growing conditions on wheat hormonal status and productivity in experimental ecological system
Колич.характеристики :6 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 50, Is. 2. - P237-242. - ISSN 1021-4437, DOI 10.1023/A:1022933516663
Примечания : Cited References: 24
Предметные рубрики: GRAINS
Ключевые слова (''Своб.индексиров.''): triticum aestivum--phytohormones--co2--illuminance--productivity
Аннотация: The levels of free and bound forms of IAA, ABA, cytokinins (CK), and gibberellins, as well as growth characteristics and productivity were investigated in two wheat lines. The plants were grown under controlled conditions in an artificial ecosystem that allowed the irradiance, CO2 concentration, and rhizosphere temperature to be changed. The main difference in the hormonal status of leaves of tall spring wheat, line 232, and dwarf wheat, line 95-3, was the absence of GA(9) gibberellins in the latter. It was found that the light intensity and temperature of rhizosphere insignificantly affected the balance of endogenous phytohormones and HI in wheat. The elevation of CO2 concentration resulted in a considerable increase in the content of free IAA, an appearance of free GA(9), and a rise in the productivity of wheat, line 232. The concentration of CO2 was shown to be a major parameter that determined HI in the experimental ecological system.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko, T. V., Shikhov, V. N., Tikhomirov, A. A.
Заглавие : Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves
Колич.характеристики :7 с
Коллективы : section VI of the Program for Basic Research of the State Academies of Sciences [56.1.4]
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 62, Is. 3. - С. 307-313. - ISSN 1021-4437, DOI 10.1134/S1021443715020144. - ISSN 1608-3407(eISSN)
Примечания : Cited References:30. - This work was supported by the State Assignment, topic no. 56.1.4, section VI of the Program for Basic Research of the State Academies of Sciences for 2013-2020.
Предметные рубрики: LEAF SENESCENCE
ONTOGENIC APPROACH
LIGHT DEPENDENCE
PARAMETERS
Ключевые слова (''Своб.индексиров.''): triticum sativus--chlorophyll fluorescence parameters--actinic light--intensity--leaf ontogeny
Аннотация: Wheat (Triticum sativus L.) seedlings of various ages (2- to 16-day-old plants) were used to study age-dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu- orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, controlled environmental conditions, and 690 A mu mol/(m(2) s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 A mu mol/(m(2) s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for measurements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the maximal photochemical quantum yield of photosystem II (F (v)/F (m)), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non-photochemical (qN and NPQ) quenching of chlorophyll fluorescence, the F (p)/F (t) ratio, and the "vitality index" (fluorescence decrease ratio, R (fd)). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 A mu mol/(m(2) s)), age-dependent changes in NPQ, F (p)/F (t), and R (fd) were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 A mu mol/(m(2) s) revealed that R (fd), NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Fedoseeva, Yu. V., Bulusheva L.G., Okotrub A.V., Kanygin M.A., Gorodetskiy D.V., Asanov I.P., Vyalikh D.V., Puzyr A.P., Bondar V.S.
Заглавие : Field emission luminescence of nanodiamonds deposited on the aligned carbon nanotube array
Колич.характеристики :7 с
Коллективы : RFBR grant [13-03-12118], bilateral Program "Russian-German Laboratory at BESSY''
Место публикации : Sci Rep: NATURE PUBLISHING GROUP, 2015. - Vol. 5. - Ст.9379. - ISSN 2045-2322, DOI 10.1038/srep09379
Примечания : Cited References:49. - The work was supported by RFBR grant 13-03-12118 in the part of electroluminescence measurements and the bilateral Program "Russian-German Laboratory at BESSY''. We are grateful to Mr. A.V. Ischenko for the TEM measurements, Mr. S.I. Kozhemyachenko for the Raman spectra, Mrs. N.I. Alferova for the IR spectra, and Mr. D.V. Gulyaev for the photoluminescence spectra.
Предметные рубрики: DETONATION NANODIAMOND
ULTRANANOCRYSTALLINE DIAMOND
Аннотация: Detonation nanodiamonds (NDs) were deposited on the surface of aligned carbon nanotubes (CNTs) by immersing a CNT array in an aqueous suspension of NDs in dimethylsulfoxide (DMSO). The structure and electronic state of the obtained CNT-ND hybrid material were studied using optical and electron microscopy and Infrared, Raman, X-ray photoelectron and near-edge X-ray absorption fine structure spectroscopy. A non-covalent interaction between NDs and CNT and preservation of vertical orientation of CNTs in the hybrid were revealed. We showed that current-voltage characteristics of the CNT-ND cathode are changed depending on the applied field; below similar to 3 V/mu m they are similar to those of the initial CNT array and at the higher field they are close to the ND behavior. Involvement of the NDs in field emission process resulted in blue luminescence of the hybrid surface at an electric field higher than 3.5 V/mu m. Photoluminescence measurements showed that the NDs emit blue-green light, while blue luminescence prevails in the CNT-ND hybrid. The quenching of green luminescence was attributed to a partial removal of oxygen-containing groups from the ND surface as the result of the hybrid synthesis.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tolomeyev A.P., Zadereev Y.S.
Заглавие : An in situ method for the investigation of vertical distributions of zooplankton in lakes: test of a two-compartment enclosure
Колич.характеристики :8 с
Место публикации : Aquat. Ecol.: SPRINGER, 2005. - Vol. 39, Is. 2. - P181-188. - ISSN 1386-2588, DOI 10.1007/s10452-004-5732-0
Примечания : Cited References: 21
Предметные рубрики: MIGRATION
DAPHNIA
RADIATION
COPEPODS
EXPOSURE
PATTERNS
LIGHT
Ключевые слова (''Своб.индексиров.''): anoxic hypolimnion--solar radiation--stratified lake--vertical migration--zooplankton
Аннотация: Two-section enclosures were designed for the investigation of the effect of various physicochemical and biological factors on vertical distribution of zooplankton in situ. The framework of the enclosure was a cylindrical polyethylene column without any partitions inside, in which the isolation of animals in different sections after in situ exposure was achieved by pinching the flexible central part of the column. Enclosures were tested at the brackish stratified meromictic Lake Shira (Russia, Khakasia). The absence of fish and carnivorous zooplankton in the lake suggests that the vertical distribution of zooplankton is mainly determined by physicochemical gradients in the water column. Experiments and field observations demonstrated that all age and size groups of Arctodiaptomus salinus and Brachionus plicatilis strongly avoided surface layers during the daylight. The escape of zooplankton from the anoxic hypolimnion was less active. Statistically significant avoidance was observed only for copepodites C4-C5 and females of A. salinus. The relatively simple construction of the columns and easy handling during the experiment were the factors that favoured the use of this device to perform in situ basic tests of the effect of different factors on the vertical distribution of zooplankton.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T. V., Shikhov V. N., Tikhomirov A. A.
Заглавие : Effect of light intensity on the age dependence of nonphotochemical fluorescence quenching in wheat leaf
Колич.характеристики :4 с
Коллективы : RAS [56.1.4]
Место публикации : Photosynthetica: SPRINGER, 2015. - Vol. 53, Is. 4. - С. 617-620. - ISSN 0300-3604, DOI 10.1007/s11099-015-0133-2. - ISSN 1573-9058(eISSN)
Примечания : Cited References:16. - The study was performed within the framework of subject No. 56.1.4., Section VI in accordance with State Program of RAS Fundamental Research for 2013-2020.
Предметные рубрики: CHLOROPHYLL FLUORESCENCE
PHOTOSYNTHESIS
LEAVES
INDUCTION
ENERGY
Ключевые слова (''Своб.индексиров.''): chlorophyll fluorescence induction--leaf age--photosystem ii
Аннотация: The effects of actinic light (AL) intensity on the age dependence of nonphotochemical fluorescence quenching (q(N)) and effective quantum yield in PSII (I broken vertical bar(PSII)) were studied in continuously illuminated wheat leaves of the upper tier. Regular changes were revealed in both age dependence of q(N) at elevated AL intensities and light curves of q(N). These changes are related to alterations in strategies of redistribution and use of absorbed light energy by the photosynthetic apparatus at different stages of wheat leaf development. Unlike I broken vertical bar(PSII), q(N) as a parameter was more sensitive to the differences in the leaf age at a certain range of light intensities. At the same time, the stability of q(N) at moderate light intensities may serve as an indication of leaf maturity.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Bondar V. S.
Заглавие : Comparative evaluation of total peroxidase and catalase activities during light emission of luminous fungus Neonothopanus nambi
Место публикации : Mycosphere: Guizhou Key Laboratory of Agricultural Biotechnology, 2016. - Vol. 7, Is. 4. - С. 499-510. - ISSN 20777000 (ISSN) , DOI 10.5943/mycosphere/7/4/9
Ключевые слова (''Своб.индексиров.''): basidiomycetes--hydrogen peroxide--luminescence--stress
Аннотация: Submerged cultivation of luminous fungus Neonothopanus nambi under orbital stirring causes formation of pellets with smooth or rough surfaces. The experiments showed that luminescence of the pellets washed in water increased considerably. Previous studies suggested possible participation of peroxidases in the light emitting reaction. In this study, oxidative azo coupling reaction accompanied by formation of chromogen was used to evaluate peroxidase activity in vivo, in brightly luminescent pellets and in pellets with low luminescence intensity (dim ones). Staining of the brightly luminescent pellets took a few minutes, and their staining intensity was several times higher than that of the dim pellets. From the results of in vivo experiments it was concluded that the bright pellets differed from the dim ones in the production of hydrogen peroxide, or, possibly, other peroxides. Measurements of total peroxidase and catalase activities in pellet extracts also showed an increase in enzyme activities along with an increase in luminescence intensity of native pellets. However, results of the in vitro experiments do not definitively suggest a direct relationship between luminescence and activity of these enzymes. We assume that luminescence of this fungal species may be an additional way to neutralize peroxide compounds under stress. © Guizhou Academy of Agricultural Sciences.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M. I., Sushchik N. N., Shulepina S. P., Ageev A. V., Dubovskaya O. P., Kolmakova A. A., Kalachova G. S.
Заглавие : Secondary Production of Highly Unsaturated Fatty Acids by Zoobenthos Across Rivers Contrasting in Temperature
Колич.характеристики :12 с
Коллективы : Siberian Federal University [6.1089.214/K]; Russian Federal Tasks of Fundamental Research [51.1.1]
Место публикации : River Res. Appl.: WILEY-BLACKWELL, 2016. - Vol. 32, Is. 6. - С. 1252-1263. - ISSN 1535-1459, DOI 10.1002/rra.2945. - ISSN 1535-1467(eISSN)
Примечания : Cited References:55. - The work was supported by the project no. 6.1089.214/K of Siberian Federal University carried out according to Federal Tasks of Ministry of Education and Science of Russian Federation and by Russian Federal Tasks of Fundamental Research (project no. 51.1.1).
Предметные рубрики: AQUATIC ECOSYSTEMS
CLIMATE-CHANGE
YENISEI RIVER
ALPINE PONDS
LAKE
Ключевые слова (''Своб.индексиров.''): essential polyunsaturated fatty acids--river zoobenthos--secondary--production--biodiversity--water temperature--climate warming
Аннотация: Highly unsaturated fatty acids (HUFA), namely eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), which are essential for many animals, including humans, are mainly produced in aquatic trophic webs. In fast-flowing rivers, macrozoobenthos is the main source of HUFA for fish and may be particularly vulnerable to thermal alterations associated with climate change. We studied benthic communities in a unique natural ecosystem: the Yenisei River downstream of the dam of Krasnoyarsk Hydroelectric Power Station with very low temperature in summer because of discharge of cold water from deep in the reservoir and its tributaries with high summer temperature. This natural experiment' allowed to get rid of confounding factors, such as differences in light, seasonality, geology (biogeochemistry) and biogeography (regional species pools). As found, in spite of an increase of biodiversity and rates of daily production in warm rivers compared with cold sites, DHA and partly EPA production of zoobenthos decreased with the increase of temperature because of changes in species composition. Thus, in a climate warming context, we can predict a decrease of production of these HUFA by river zoobenthos and thereby a diminishing of their supply for fish and next to humans. Copyright (c) 2015 John Wiley & Sons, Ltd.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova L. P., Natashin P. V., Markova S. V., Eremeeva E. V., Malikova N. P., Cheng C., Liu Z. -J., Vysotski E. S.
Заглавие : Mitrocomin from the jellyfish Mitrocoma cellularia with deleted C-terminal tyrosine reveals a higher bioluminescence activity compared to wild type photoprotein
Место публикации : J. Photochem. Photobiol. B Biol. - 2016. - Vol. 162. - С. 286-297. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2016.06.054
Аннотация: The full-length cDNA genes encoding five new isoforms of Ca2 +-regulated photoprotein mitrocomin from a small tissue sample of the outer bell margin containing photocytes of only one specimen of the luminous jellyfish Mitrocoma cellularia were cloned, sequenced, and characterized after their expression in Escherichia coli and subsequent purification. The analysis of cDNA nucleotide sequences encoding mitrocomin isoforms allowed suggestion that two isoforms might be the products of two allelic genes differing in one amino acid residue (64R/Q) whereas other isotypes appear as a result of transcriptional mutations. In addition, the crystal structure of mitrocomin was determined at 1.30 A resolution which expectedly revealed a high similarity with the structures of other hydromedusan photoproteins. Although mitrocomin isoforms reveal a high degree of identity of amino acid sequences, they vary in specific bioluminescence activities. At that, all isotypes displayed the identical bioluminescence spectra (473–474 nm with no shoulder at 400 nm). Fluorescence spectra of Ca2 +-discharged mitrocomins were almost identical to their light emission spectra similar to the case of Ca2 +-discharged aequorin, but different from Ca2 +-discharged obelins and clytin which fluorescence is red-shifted by 25–30 nm from bioluminescence spectra. The main distinction of mitrocomin from other hydromedusan photoproteins is an additional Tyr at the C-terminus. Using site-directed mutagenesis, we showed that this Tyr is not important for bioluminescence because its deletion even increases specific activity and efficiency of apo-mitrocomin conversion into active photoprotein, in contrast to C-terminal Pro of other photoproteins. Since genes in a population generally exist as different isoforms, it makes us anticipate the cloning of even more isoforms of mitrocomin and other hydromedusan photoproteins with different bioluminescence properties. © 2016 Elsevier B.V.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Fu, Yuming, Li, HongYan, Yu, Juan, Liu, Hui, Cao, ZeYu, Manukovsky N. S., Liu, Hui
Заглавие : Interaction effects of light intensity and nitrogen concentration on growth, photosynthetic characteristics and quality of lettuce (Lactuca sativa L. Var. youmaicai)
Колич.характеристики :7 с
Коллективы : Ministry of Science and Technology of China [2013AA103004]; National Natural Science Foundation of China [31301706]
Место публикации : Sci. Hortic.: ELSEVIER SCIENCE BV, 2017. - Vol. 214. - С. 51-57. - ISSN 0304-4238, DOI 10.1016/j.scienta.2016.11.020. - ISSN 1879-1018(eISSN)
Примечания : Cited References:43. - This work was supported by the Ministry of Science and Technology of China (No. 2013AA103004) and National Natural Science Foundation of China (No. 31301706).
Предметные рубрики: WATER-USE EFFICIENCY
LIFE-SUPPORT-SYSTEM
GAS-EXCHANGE
BLUE-LIGHT
Ключевые слова (''Своб.индексиров.''): light intensity--nitrogen concentration--vitamin c--vegetable quality--facility agriculture
Аннотация: Light intensity and nitrogen concentration of nutrient solution are considered crucial for the contents of vitamin C and nitrate in cultivated leaf vegetables. We here investigated the effects of various combinations of light intensity (60,140 and 220 mu mol m(-2) s(-1)) and nitrogen concentration (7,15 and 23 mmol L-1) of the solution on the growth, photosynthetic characteristics, vitamin C and nitrate content of lettuce. Our results demonstrate that the lettuce had the largest dry biomass at the high illumination of 220 mu mol m(-2) s(-1) and low nitrogen of 7 mmol L-1. The higher light and low nitrogen also contributed to the accumulation of vitamin C and decrease of nitrate in lettuce leaves. The effect of nitrogen supply on chlorophyll concentrations was more efficient under low light than high illumination. Our results reveal that there was an obvious interaction between light intensity and nitrogen available for the photosynthesis, yield and quality of lettuce. This study provides valuable insights into the combinational regulation of light intensity and nitrogen supply for improving growth and nutritional quality of vegetables grown in greenhouse and plant factory. (C) 2016 Elsevier B.V. All rights reserved.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E.
Заглавие : Luminescence of wood samples during long-term storage
Место публикации : Mycosphere: Guizhou Key Laboratory of Agricultural Biotechnology, 2016. - Vol. 7, Is. 6. - С. 716-727. - ISSN 20777000 (ISSN) , DOI 10.5943/mycosphere/7/6/2
Ключевые слова (''Своб.индексиров.''): light emitting wood--luminous mycelia
Аннотация: The present study describes changes in the mycelium of the fungus growing on the luminescent wood collected on Borneo Island in early December 2013 that occurred during 31 months of storage. The study shows that wood samples retain their ability to emit light, forming two types of luminescent mycelium: surface mycelium and aerial mycelium. The hyphae of the surface mycelium form on the surface of the wood sample and then spread over the surface of the polyethylene bag or over the surface of the bottom of tissue culture flasks containing the samples. The aerial mycelium develops later and only in tissue culture flasks, forming biomass composed of local interlaced hyphae, growing upward. The surface mycelium is characterized by non-uniform "flickering" luminescence along the hyphae. There is no diurnal periodicity in the luminescence of this fungus, but luminescence is increased by mechanical disturbance or exposure to ultraviolet radiation. The local impact of these factors causes an increase in luminescence of the mycelium regions that have not been directly affected. It has been assumed that the variable level of luminescence is an individual trait of this fungus species. The results obtained in this study suggest that luminescent wood found on Borneo Island contains mycelium of a fungus species, whose luminescent properties are essentially different from those of the fungi described in the scientific literature.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Deeva, Anna A., Temlyakova, Evgenia A., Sorokin, Anatoly A., Nemtseva, Elena V., Kratasyuk, Valentina A.
Заглавие : Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis
Колич.характеристики :5 с
Коллективы : RFBR [16-34-00746 mol_a]; Ministry of Education and Science of the Russian Federation [1762]; state budget allocated to the fundamental research at Russian Academy of Sciences [01201351504]
Место публикации : Bioinformatics: OXFORD UNIV PRESS, 2016. - Vol. 32, Is. 20. - С. 3053-3057. - ISSN 1367-4803, DOI 10.1093/bioinformatics/btw386. - ISSN 1460-2059(eISSN)
Примечания : Cited References:31. - The reported study was partially funded by RFBR according to the research project No. 16-34-00746 mol_a; by the Ministry of Education and Science of the Russian Federation [project No 1762] and by the state budget allocated to the fundamental research at the Russian Academy of Sciences [project No 01201351504].
Аннотация: Motivation: Bacterial luciferases are heterodimeric enzymes that catalyze a chemical reaction, so called bioluminescence, which causes light emission in bacteria. Bioluminescence is vastly used as a reporter system in research tools and commercial developments. However, the details of the mechanisms that stabilize and transform the reaction intermediates as well as differences in the enzymatic kinetics amongst different bacterial luciferases remain to be elucidated. Results: Amino acid sequences alignments for 21 bacterial luciferases (both alpha- and beta-subunits) were analyzed. For alpha-subunit, containing the enzyme active center, 48 polymorphic amino acid positions were identified. According to them, the sequences fell into two distinct groups known as slow and fast based on the decay rate of the bioluminescence reaction. The differences in the enzyme active site induced by structural polymorphism are analyzed.
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