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241Am distribution in the biomass of freshwater macrophytes / T. A. Zotina [et al.] // Doklady Biological Sciences. - 2008. - Vol. 421, Is. 1. - P254-256, DOI 10.1134/S0012496608040108 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- carbohydrate -- cellulose -- lipid -- nitrogen -- polysaccharide -- vegetable protein -- article -- biomass -- Bryopsida -- cell membrane -- cell wall -- chemistry -- cytoplasm -- food chain -- growth, development and aging -- Hydrocharitaceae -- metabolism -- Americium -- Biomass -- Bryopsida -- Carbohydrates -- Cell Membrane -- Cell Wall -- Cellulose -- Cytoplasm -- Food Chain -- Hydrocharitaceae -- Lipids -- Nitrogen -- Plant Proteins -- Polysaccharides

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Kalachova, G.S.; Bolsunovsky, A.Ya.; Degermendzhy, A.G.

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A comparison of the fatty acid composition of Gammarus lacustris and its food sources from a freshwater reservoir, Bugach, and the saline Lake Shira in Siberia, Russia [Text] / O. N. Makhutova, G. S. Kalachova, M. I. Gladyshev // Aquat. Ecol. - 2003. - Vol. 37, Is. 2. - P. 159-167, DOI 10.1023/A:1023975101075. - Cited References: 26 . - ISSN 1386-2588

РУБ Ecology + Limnology + Marine & Freshwater Biology
Рубрики:
SEASONAL-VARIATION
   LIPID-COMPOSITION
   WATER FISH
   AMPHIPODA
   RESOURCES
   ERKEN
   POND
Кл.слова (ненормированные):
fatty acid composition -- freshwater reservoir -- Gammarus lacustris -- gut content -- saline lake -- sediments -- Seston
Аннотация: We studied fatty acid (FA) composition in samples from bodies and intestinal contents of the littoral amphipod Gammarus lacustris Sars, from the Bugach freshwater reservoir. Simultaneously, samples of seston and bottom sediments were also collected from the reservoir during early August. There were no differences in FA composition of gut contents, seston and sediments of pebbly bottom. Seston was the main food source of Gammarus but some FAs Gammarus got from sediments. The FA composition of G. lacustris and seston from the Bugach freshwater reservoir were compared with those of the animals from the saltwater Lake Shira (Siberia). While FA composition of the two Gammarus populations differed significantly, those of seston were practically similar: the composition of long-chain unsaturated fatty acids, 20:5omega3, 22:6omega3 and 20:4omega6, were significantly higher in animals from saline Shira Lake, whereas 16:1 and 16:0 were higher in the freshwater populations of amphipods from the Bugach freshwater reservoir. Taking into account the relevant literature data, we hypothesise that this difference in C16 acid might be a distinguishing characteristic of FA composition of freshwater and saltwater crustaceans.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660042, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Makhutova, O.N.; Kalachova, G.S.; Gladyshev, M.I.

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Agglomeration behavior of lipid-capped gold nanoparticles / R. Ranjan [et al.] // J. Nanopart. Res. - 2018. - Vol. 20, Is. 4. - Ст. 107, DOI 10.1007/s11051-018-4215-5. - Cited References:35. - The research was supported by the Russian Foundation for Basic Research [project no. 16-34-60100] and the state budget allocated to the fundamental research (project no. 0356-2017-0017). The authors thank Prof. Tatiana Volova, Prof. Evgenia Slyusareva, and Ms. Nina Slyusarenko of the Siberian Federal University for their assistance in the zeta potential and zeta-average analysis. . - ISSN 1388-0764. - ISSN 1572-896X

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
COLORIMETRIC SENSOR ARRAY
   OPTICAL-PROPERTIES
   AGGREGATION
   CANCER
Кл.слова (ненормированные):
Gold nanoparticles -- Ionic interference -- Agglomeration -- Stabilization -- Lipid capping -- Nanobiotechnology applications
Аннотация: The current investigation deciphers aggregation pattern of gold nanoparticles (AuNPs) and lipid-treated AuNPs when subjected to aqueous sodium chloride solution with increasing ionic strengths (100-400 nM). AuNPs were synthesized using 0.29 mM chloroauric acid and by varying the concentrations of trisodium citrate (AuNP1 1.55 mM, AuNP2 3.1 mM) and silver nitrate (AuNP3 5.3 mu M, AuNP4 10.6 mu M) with characteristic LSPR peaks in the range of 525-533 nm. TEM analysis revealed AuNPs to be predominantly faceted nanocrystals with the average size of AuNP1 to be 35 +/- 5 nm, AuNP2 15 +/- 5 nm, AuNP3 30 +/- 5 nm, and AuNP4 30 +/- 5 nm and the zeta-average for AuNPs were calculated to be 31.23, 63.80, 26.08, and 28 nm respectively. Induced aggregation was observed within 10 s in all synthesized AuNPs while lipid-treated AuNP2 (AuNP2-L) was found to withstand ionic interferences at all concentration levels. However, lipid-treated AuNPs synthesized using silver nitrate and 1.55 mM trisodium citrate (AuNP3, AuNP4) showed much lower stability. The zeta potential values of lipid-treated AuNPs (AuNP1-L-1x/200, -17.93 +/- 1.02 mV; AuNP2-L-1x/200, -21.63 +/- 0.70; AuNP3-L-1x/200, -14.54 +/- 0.90; AuNP3-L-1x/200 -13.77 +/- 0.83) justified these observations. To summarize, AuNP1 and AuNP2 treated with lipid mixture 1 equals or above 1x/200 or 1x/1000 respectively showed strong resistance against ionic interferences (up to 400 mM NaCl). Use of lipid mixture 1 for obtaining highly stable AuNPs also provided functional arms of various lengths which can be used for covalent coupling.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, 79 Svobodny Prospect, Krasnoyarsk 660041, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Kirensky Inst Phys, Akademgorodok 50-38, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Electron Microscopy Lab, 79 Svobodny Prospect, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Ranjan, Rajeev; Kirillova, Maria A.; Esimbekova, Elena N.; Zharkov, Sergey M.; Kratasyuk, Valentina A.; Russian Foundation for Basic Research [16-34-60100]; [0356-2017-0017]

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Assessing the reliability of quantitative fatty acid signature analysis and compound-specific isotope analysis-based mixing models for trophic studies / I. Prokopkin, O. Makhutova, E. Kravchuk [et al.] // Biomolecules. - 2021. - Vol. 11, Is. 11. - Ст. 1590, DOI 10.3390/biom11111590 . - ISSN 2218-273X
Кл.слова (ненормированные):
CSIA?based mixing model -- Daphnia -- Fatty acids -- Food -- IsoError -- QFASA -- fatty acid -- algal cell culture -- animal experiment -- Article -- Chlorella -- compound specific isotope analysis -- controlled study -- Cryptomonas -- Daphnia -- fatty acid analysis -- gas chromatography -- isotope analysis -- lipid composition -- mathematical model -- nonhuman -- quantitative fatty acid signature analysis -- reliability -- zooplankton
Аннотация: The study of the trophic relationships of aquatic animals requires correct estimates of their diets. We compared the quantitative fatty acid signature analysis (QFASA) and the isotope?mixing model IsoError, based on the compound?specific isotope analysis of fatty acids (CSIA?FA), which are potentially effective models for quantitative diet estimations. In a 21?day experiment, Daphnia was fed a mixture of two food items, Chlorella and Cryptomonas, which were supplied in nearly equal proportions. The percentages and isotope values of the FAs of the algal species and Daphnia were measured. The IsoError based on CSIA?FA gave an estimation of algae consumption using only one FA, 18:3n?3. According to this model, the proportion of consumption of Chlorella decreased while the proportion of consumption of Cryptomonas increased during the experiment. The QFASA model was used for two FA subsets—the extended?dietary subset, which included sixteen FAs, and the dietary one, which included nine FAs. According to both subsets, the portion of consumed Chlorella decreased from Day 5 to 10 and then increased at Day 21. The comparison of the two model approaches showed that the QFASA model is a more reliable method to determine the contribution of different food sources to the diet of zooplankton than the CSIA?based mixing model. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Prokopkin, I.; Makhutova, O.; Kravchuk, E.; Sushchik, N.; Anishchenko, O.; Gladyshev, M.

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Biochemical composition of several blue green algae and Chlorella / N. I. Trubachev, I. I. Gitel'zon, G. S. Kalacheva // Applied Biochemistry and Microbiology. - 1977. - Vol. 12, Is. 2. - P155-161 . - ISSN 0003-6838
Кл.слова (ненормированные):
fatty acid -- lipid -- protein -- vitamin -- alga -- blue green alga -- chlorella -- in vitro study -- microorganism -- plant -- theoretical study

Scopus
Держатели документа:
Inst. Phys., Siberian Branch, Acad. Scis USSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trubachev, N.I.; Gitel'zon, I.I.; Kalacheva, G.S.

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Biochemical fractionation and cellular distribution of americium and plutonium in the biomass of freshwater macrophytes / T. A. Zotina, G. S. Kalacheva, A. Y. Bolsunovsky // Journal of Radioanalytical and Nuclear Chemistry. - 2011. - Vol. 290, Is. 2. - P447-451, DOI 10.1007/s10967-011-1228-2 . - ISSN 0236-5731
Кл.слова (ненормированные):
238, 242Pu -- 241Am -- Carbohydrates -- Cellulose -- Fractionation -- Protein -- Submerged macrophyte -- americium 241 -- plutonium -- plutonium 238 -- plutonium 242 -- polysaccharide -- unclassified drug -- article -- bioaccumulation -- biomass -- cellular distribution -- Ceratophyllum demersum -- controlled study -- cytosol -- Elodea canadensis -- Fontinalis antipyretica -- fractionation -- freshwater species -- macrophyte -- moss -- Myriophyllum spicatum -- nonhuman -- plant cell -- radiation absorption -- radioactivity -- shoot
Аннотация: Accumulation of americium ( 241Am) and plutonium ( 238,242Pu) and their distribution in cell compartments and biochemical components of the biomass of freshwater aquatic plants Elodea canadensis, Ceratophyllum demersum and Myrioplyllum spicatum and aquatic moss Fontinalis antipyretica have been investigated in laboratory experiments. Americium and plutonium taken up from water by Elodea canadensis apical shoots were mainly absorbed by structural components of plant cells (90% for 241Am; 89% for 238Pu and 82-87% for 242Pu). About 10-18% of isotope activity was recorded in the cytosol fraction. The major concentration (76-92%) of americium was bound to cell wall cellulose-like polysaccharides of Elodea canadensis, Myriophyllum spicatum, Ceratophyllum demersum and Fontinalis antipyretica, 8-24% of americium activity was registered in the fraction of proteins and carbohydrates, and just a minor concentration (<1%) in the lipid fraction. The distribution of plutonium in the biomass fractions of Elodea was similar to that of americium. Hence, americium and plutonium had the highest affinity to cellulose-like polysaccharides of cell walls of freshwater submerged macrophytes. В© 2011 Akademiai Kiado, Budapest, Hungary.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Kalacheva, G.S.; Bolsunovsky, A.Y.

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Biodiesel production from sediments of a eutrophic reservoir / A. Kuchkina [et al.] // Biomass and Bioenergy. - 2011. - Vol. 35, Is. 5. - P2280-2284, DOI 10.1016/j.biombioe.2011.02.047 . - ISSN 0961-9534
Кл.слова (ненормированные):
Biodiesel -- Dredging sediments -- Eutrophic reservoir -- Fatty acids -- Lipids -- Bio-diesel fuel -- Biodiesel production -- Cetane number -- Dry weight -- EN 14214 -- Eutrophic reservoirs -- Heat of combustion -- High price -- Iodine number -- IS costs -- Lake restoration -- Lipid content -- Production cost -- SIBERIA -- Anoxic sediments -- Biodiesel -- Costs -- Diesel fuels -- Eutrophication -- Fatty acids -- Iodine -- Lipids -- Reservoirs (water) -- Sedimentology -- Synthetic fuels -- Thermochemistry -- Dredging -- biofuel -- combustion -- dredging -- environmental restoration -- eutrophic environment -- iodine -- lacustrine deposit -- lipid -- production cost -- reservoir -- Bugach Reservoir -- Krasnoyarsk [Russian Federation] -- Russian Federation
Аннотация: Sediments from eutrophic reservoir Bugach (Siberia, Russia) were tested for possibility to produce biodiesel. We supposed that the sediments could be a promising biodiesel producer. The major reason of high price of biodiesel fuel is cost of a raw material. The use of dredging sediments for biodiesel production reduces production costs, because the dredging sediments are by-products which originated during lake restoration actions, and are free of cost raw materials. Lipid content in sediments was 0.24% of dry weight. To assess the potential of from sediments as a substitute of diesel fuel, the properties of the biodiesel such as cetane number, iodine number and heat of combustion were calculated. All of this parameters complied with limits established by EN 14214 and EN 14213 related to biodiesel quality. В© 2011 Elsevier Ltd.

Scopus
Держатели документа:
Siberian Federal University, Svobodny av. 79, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics Siberian Branch of RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kuchkina, A.; Gladyshev, M.I.; Sushchik, N.N.; Kravchuk, E.S.; Kalachova, G.S.

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Bioluminescent analysis of intensity of pathological oxidative processes in cells of perfused rat liver after hyperthermia / N. N. Remmel [et al.] // Bulletin of Experimental Biology and Medicine. - 2003. - Vol. 135, Is. 1. - P. 43-45, DOI 10.1023/A:1023489727703 . - ISSN 0007-4888
Кл.слова (ненормированные):
Bioluminescent analysis -- Controlled perfusion of isolated liver -- Hyperthermia oxidative stress -- thiobarbituric acid reactive substance -- animal experiment -- animal model -- animal tissue -- article -- bioluminescence -- concentration (parameters) -- controlled study -- correlation analysis -- female -- hyperthermia -- in vivo study -- lipid peroxidation -- liver metabolism -- liver perfusion -- male -- nonhuman -- oxidative stress -- oxygen consumption -- pathology -- rat -- thermal exposure -- Animals -- Female -- Hepatocytes -- Hyperthermia, Induced -- Liver -- Luminescent Measurements -- Male -- Oxidative Stress -- Perfusion -- Rats -- Animalia
Аннотация: We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation
Intl. Res. Ctr. of Extreme States, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Remmel, N.N.; Kratasyuk, V.A.; Maznyak, O.M.; Inzhevatkin, E.V.; Nefedov, V.P.

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Changes of lipids and their fatty acid composition during batch cultivation of the luminescent bacterium / G. S. Kalacheva, E. S. Vysotsky // Mikrobiologiya. - 1984. - Vol. 53, Is. 6. - С. 932-937 . - ISSN 0026-3656
Кл.слова (ненормированные):
fatty acid -- lipid -- bacterium -- bioluminescence -- nonhuman

Scopus
Держатели документа:
Institut Biofiziki, SO AN SSSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Vysotsky, E.S.

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10.

Characteristics of lipids from green alga Botryococcus braunii synthesizing liquid hydrocarbons. / T. G. Volova, G. S. Kalacheva, N. O. Zhyla // Doklady Biological Sciences. - 2000. - Vol. 370, Is. 1-6. - P96-99 . - ISSN 0012-4966
Кл.слова (ненормированные):
fatty acid -- hydrocarbon -- lipid -- article -- biomass -- chemistry -- green alga -- metabolism -- Algae, Green -- Biomass -- Fatty Acids -- Hydrocarbons -- Lipids

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Zhyla, N.O.

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11.

Chemical composition of water conditioned by crowded Moina macracopa (Crustacea : Cladocera) [Text] / G. S. Kalacheva [et al.] // Russ. J. Ecol. - 2000. - Vol. 31, Is. 2. - P. 113-117, DOI 10.1007/BF02828365. - Cited References: 25 . - ISSN 1067-4136

РУБ Ecology
Рубрики:
DAPHNIA-PULEX
   ZOOPLANKTON
   PARAMETERS
   INDUCTION
Кл.слова (ненормированные):
Cladocera -- metabolic products -- chemical analysis -- chemical composition
Аннотация: The chemical composition of water conditioned by colonies of Moina macrocopa and involved in the regulation of their life cycle was analyzed in comparison with water samples from natural water bodies. Dechlorinated tap water was used as the control medium. The results showed that metabolism of crustaceans leads to an increase in the concentrations of phosphorus and nitrogen in all forms, the appearance of protein and carbohydrate fractions, and changes in the composition of lipid fractions in the water conditioned by their colonies. The contents of all organic components are very low, which makes their identification difficult. Concentrations of substances identified in the conditioned water do not exceed those occurring in nature.

WOS
Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zadereev, E.S.; Gubanov, V.G.; Gribovskaya, I.V.

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12.

Chemical composition of water conditioned by crowded Moina macrocopa (Crustacea: Cladocera) / G. S. Kalacheva [et al.] // Russian Journal of Ecology. - 2000. - Vol. 31, Is. 2. - P113-117 . - ISSN 1067-4136
Кл.слова (ненормированные):
Chemical analysis -- Chemical composition -- Cladocera -- Metabolic products
Аннотация: The chemical composition of water conditioned by colonies of Moina macrocopa and involved in the regulation of their life cycle was analyzed in comparison with water samples from natural water bodies. Dechlorinated tap water was used as the control medium. The results showed that metabolism of crustaceans leads to an increase in the concentrations of phosphorus and nitrogen in all forms, the appearance of protein and carbohydrate fractions, and changes in the composition of lipid fractions in the water conditioned by their colonies. The contents of all organic components are very low, which makes their identification difficult. Concentrations of substances identified in the conditioned water do not exceed those occurring in nature. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zadereev, E.S.; Gubanov, V.G.; Gribovskaya, I.V.

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13.

Comparison of fatty acid composition in major lipid classes of the dominant benthic invertebrates of the Yenisei river [Text] / N. N. Sushchik [et al.] // Comp. Biochem. Physiol. B-Biochem. Mol. Biol. - 2003. - Vol. 134, Is. 1. - P. 111-122, DOI 10.1016/S1096-4959(02)00191-4. - Cited References: 30 . - ISSN 1096-4959

РУБ Biochemistry & Molecular Biology + Zoology
Рубрики:
LAKE-ERKEN
   BIOMARKERS
   BACTERIA
   MARKERS
   DIET
   FISH
   N-3
Кл.слова (ненормированные):
fatty acids -- triacylglycerols -- polar lipids -- gammarids -- aquatic larvae of insects
Аннотация: The composition and content of fatty acids (FAs) in total lipids, triacylglycerols (TAG) and polar lipids (PL) in dominant groups of benthic invertebrates: gammarids (Gammaridae, Amphipoda), chironomid larvae (Chironomidae, Diptera), caddisfly larvae (Trichoptera) and mayfly larvae (Ephemeroptera) were studied in the Yenisei river. For the first time data on the FA composition of species belonging to Trichoptera (Insecta) are presented. The groups of aquatic insect larvae and gammarids weakly differed in total content of essential polyunsaturated fatty acids (PUFAs). Hence, the strong invasion of gammarids which occurred in the last decades in the Yenisei river should not result in a decrease in potential yield of essential PUFA in the ecosystem and corresponding decrease in food resource quality for fish in respect to PUFA content. Significant differences in biomarker FAs in TAG were found which correlated to specific food sources. Different levels of long-chain PUFA in PL of the invertebrates are discussed in relation to the genetic ability of particular taxa to form these FAs. (C) 2002 Elsevier Science Inc. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Gladyshev, M.I.; Moskvichova, A.V.; Makhutova, O.N.; Kalachova, G.S.

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14.

Comparison of fatty acid contents and composition in major lipid classes of larvae and adults of mosquitoes (Diptera: Culicidae) from a steppe region / N. N. Sushchik [et al.] // Insect Science. - 2013. - Vol. 20, Is. 5. - P585-600, DOI 10.1111/j.1744-7917.2012.01582.x . - ISSN 1672-9609
Кл.слова (ненормированные):
Amphibiotic insects -- Essential fatty acids -- Metamorphosis -- Mosquitoes -- Short-chain polyunsaturated fatty acids
Аннотация: Emerging aquatic insects, including mosquitoes, are known to transfer to terrestrial ecosystems specific essential biochemicals, such as polyunsaturated fatty acids (PUFA). We studied fatty acid (FA) composition and contents of dominant mosquito populations (Diptera: Culicidae), that is, Anopheles messeae, Ochlerotatus caspius, Oc. flavescens, Oc. euedes, Oc. subdiversus, Oc. cataphylla, and Aedes cinereus, inhabited a steppe wetland of a temperate climate zone to fill up the gap in their lipid knowledge. The polar lipid and triacylglycerol fractions of larvae and adults were compared. In most studied mosquito species, we first found and identified a number of short-chain PUFA, for example, prominent 14:2n-6 and 14:3n-3, which were not earlier documented in living organisms. These PUFA, although occurred in low levels in adult mosquitoes, can be potentially used as markers of mosquito biomass in terrestrial food webs. We hypothesize that these acids might be synthesized (or retroconverted) by the mosquitoes. Using FA trophic markers accumulated in triacylglycerols, trophic relations of the mosquitoes were accessed. The larval diet comprised green algae, cryptophytes, and dinoflagellates and provided the mosquitoes with essential n-3 PUFA, linolenic, and eicosapentaenoic acids. As a result, both larvae and adults of the studied mosquitoes had comparatively high content of the essential PUFA. Comparison of FA proportions in polar lipids versus storage lipids shown that during mosquito metamorphosis transfer of essential eicosapentaenoic and arachidonic acids from the reserve in storage lipids of larvae to functional polar lipids in adults occurred. В© 2012 Institute of Zoology, Chinese Academy of Sciences.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, 50/50, Krasnoyarsk, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Systematics, Ecology of Animals of Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Yurchenko, Y.A.; Gladyshev, M.I.; Belevich, O.E.; Kalachova, G.S.; Kolmakova, A.A.

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15.

Comparison of Fatty Acid Contents in Major Lipid Classes of Seven Salmonid Species from Siberian Arctic Lakes / N. N. Sushchik, O. N. Makhutova, A. E. Rudchenko [et al.] // Biomolecules. - 2020. - Vol. 10, Is. 3. - Ст. 419, DOI 10.3390/biom10030419. - Cited References:60. - This research was funded by the Russian Science Foundation, grant number 16-14-10001. . - ISSN 2218-273X

РУБ Biochemistry & Molecular Biology
Рубрики:
LONG-CHAIN
   FISH
   QUALITY
   OMEGA-3
   MARINE
   WILD
   TISSUE
   FOOD
Кл.слова (ненормированные):
arctic -- Salmoniformes -- long-chain polyunsaturated fatty acids -- polar -- lipids -- triacylglycerols -- eicosapentaenoic acid -- docosahexaenoic acid
Аннотация: Long-chain omega-3 polyunsaturated fatty acids (LC-PUFA) essential for human nutrition are mostly obtained from wild-caught fish. To sustain the LC-PUFA supply from natural populations, one needs to know how environmental and intrinsic factors affect fish fatty acid (FA) profiles and contents. We studied seven Salmoniformes species from two arctic lakes. We aimed to estimate differences in the FA composition of total lipids and two major lipid classes, polar lipids (PL) and triacylglycerols (TAG), among the species and to evaluate LC-PUFA contents corresponding to PL and TAG in muscles. Fatty acid profiles of PL and TAG in all species were characterized by the prevalence of omega-3 LC-PUFA and C16-C18 monoenoic FA, respectively. Fish with similar feeding spectra were identified similarly in multivariate analyses of total lipids, TAG and PL, due to differences in levels of mostly the same FA. Thus, the suitability of both TAG and total lipids for the identification of the feeding spectra of fish was confirmed. All species had similar content of LC-PUFA esterified as PL, 1.9-3.5 mg g(-1), while the content of the TAG form strongly varied, from 0.9 to 9.8 mg g(-1). The LC-PUFA-rich fish species accumulated these valuable compounds predominately in the TAG form.

WOS
Держатели документа:
Russian Acad Sci, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys,Siberian Branch, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny Ave 79, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Sushchik, Nadezhda N.; Makhutova, Olesia N.; Rudchenko, Anastasia E.; Glushchenko, Larisa A.; Shulepina, Svetlana P.; Kolmakova, Anzhelika A.; Gladyshev, Michail I.; Makhutova, Olesia; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10001]

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16.

COMPOSITION OF THE LIPID OF A LUMINOUS CELL-FREE-EXTRACT FROM LUMINESCENT BACTERIA [Text] / G. S. KALACHEVA, E. S. VYSOTSKII, V. V. MEZHEVIKIN // Biochem.-Moscow. - 1985. - Vol. 50, Is. 11. - P. 1544-1549. - Cited References: 25 . - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VYSOTSKII, E.S.; MEZHEVIKIN, V.V.

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17.

COMPOSITION OF THE LIPID OF A LUMINOUS CELL-FREE-EXTRACT FROM LUMINESCENT BACTERIA [Text] / G. S. KALACHEVA, E. S. VYSOTSKII, V. V. MEZHEVIKIN // Biochem.-Moscow. - 1985. - Vol. 50, Is. 11. - P1544-1549. - Cited References: 25 . - 6. - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VYSOTSKII, E.S.; MEZHEVIKIN, V.V.

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18.

Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota / N. S. Rodionova, V. N. Petushkov // Journal of Photochemistry and Photobiology B: Biology. - 2006. - Vol. 83, Is. 2. - P123-128, DOI 10.1016/j.jphotobiol.2005.12.014 . - ISSN 1011-1344
Кл.слова (ненормированные):
ATP -- Bioluminescence -- Earthworms -- Ions -- Luciferin-luciferase systems -- Triton X-100 -- adenosine triphosphate -- anion -- bromine -- calcium ion -- carbonic acid -- cation -- chloride -- chromium derivative -- detergent -- dodecyl sulfate sodium -- inorganic salt -- iodine -- iron derivative -- luciferase -- luciferin -- magnesium ion -- manganese -- nitrate -- phosphate -- sulfate -- sulfite -- triton x 100 -- annelid worm -- article -- bioluminescence -- concentration (parameters) -- controlled study -- enzyme activation -- enzyme activity -- enzyme inhibition -- enzyme mechanism -- in vitro study -- nonhuman -- priority journal -- qualitative analysis -- quantitative analysis -- Adenosine Triphosphate -- Animals -- Cations, Divalent -- Cations, Monovalent -- Detergents -- Firefly Luciferin -- Kinetics -- Luciferases -- Luminescence -- Metals -- Oligochaeta -- Photobiology -- Salts -- Annelida -- Clitellata -- earthworms (sp.) -- Enchytraeidae -- Fridericia heliota -- Oligochaeta (Metazoa) -- Pheretima sieboldi
Аннотация: The study addresses the effect produced by different inorganic salts and detergents (SDS, Triton X-100, the Tween series) on the ATP-dependent bioluminescent reaction catalyzed by the luciferase of the new earthworm species Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae). It has been shown that the effect of divalent metal salts on luminescence is determined by the action of cations. Three of them - Mg2+, Mn2+ and Ca2+ - can stimulate luciferase activity at concentrations varying within a wide range, and Mn2+ can act as a 100%-effective substitute for Mg2+ in F. heliota luminescence reaction in vitro. The inhibitory effect of monovalent metal salts on luminescence is largely determined by the action of the anion part of the molecule. The effectiveness of the inhibitory effect of anions increases in the following order: {Mathematical expression}. Of the sodium salts, dodecyl sulfate, which is an anionic detergent, produces the strongest inhibitory effect on luciferase. On the contrary, nonionic detergents produce a stimulatory effect on the F. heliota luciferase. The action of the most effective of them - Triton X-100 - is determined by its ability to reduce the actual concentration of lipid inhibitors in the reaction mixture. В© 2006 Elsevier B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodionova, N.S.; Petushkov, V.N.

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19.

Effect of nitrogen limitation on the growth and lipid composition of the green alga Botryococcus braunii Kutz IPPAS H-252 / N. O. Zhila, G. S. Kalacheva, T. G. Volova // Russian Journal of Plant Physiology. - 2005. - Vol. 52, Is. 3. - P311-319, DOI 10.1007/s11183-005-0047-0 . - ISSN 1021-4437
Кл.слова (ненормированные):
Botryococcus -- Lipids -- Nitrogen limitation -- algae -- Botryococcus -- Botryococcus braunii -- Chlorophyta
Аннотация: The effect of nitrogen limitation in a medium on the composition of intracellular lipids in the alga Botryococcus braunii Kutz IPPAS H-252 in the course of culture development was investigated. Under the conditions of nitrogen limitation, the alga under investigation accumulated lipids as triacylglycerols, and this process was accompanied by substantial changes in the total fatty acid (FA) composition, which were manifested in a decrease in trienoic acids (from 52.8-57.2 to 19.5-24.7% of total FAs) and an increase in the content of oleic (from 1.1-1.2 to 17.1-24.4%) and saturated (from 23.7-26.0 to 32.9-46.1%) acids. In the control culture, the directionality of FA redistribution was less marked, and these changes were noticed at the later stages of culture development. Under nitrogen limitation, marked changes in the FA composition of polar lipids occurred by the 13th day, and they were characterized by an increase in the content of saturated acids (up to 76.8%) and a dramatic decrease in the content of all polyenoic acids (up to 6.8%). The changes in the FA composition of triacylglycerols were noticed as early as by the 7th day; these changes consisted in an increase in the content of oleic acid, and its high content (28.4-38.4%) was maintained up to the end of culturing. In the control culture, triacylglycerols with a high content of oleic acid were found by the 13th day, although, by this time, the content of total lipids and triacylglycerols did not change. В© 2005 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhila, N.O.; Kalacheva, G.S.; Volova, T.G.

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20.

Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kutz IPPAS H-252 / N. O. Zhila, G. S. Kalacheva, T. G. Volova // Journal of Applied Phycology. - 2011. - Vol. 23, Is. 1. - P47-52, DOI 10.1007/s10811-010-9532-8 . - ISSN 0921-8971
Кл.слова (ненормированные):
Botryococcus -- Fatty acid composition -- Lipid content -- Salinity -- algae -- Botryococcus -- Botryococcus braunii
Аннотация: The effect of 0.3 and 0.7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, and fatty acid profile of the lipids of the alga Botryococcus braunii IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first 3 days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols, and considerable changes in the lipid fatty acid profile: decreases in polyenoic acid contents (from 68.34% to 29.38% and 12.8%) and proportions of long-chain saturated acids (from 0.53% to 5.3% and 14.13% of the total fatty acids) at 0.3 M NaCl and 0.7 M NaCl, respectively. In later phases of the culture, at 0.3 M NaCl, the content of polyenoic acids rose to the values characteristic of the active growth phase of this alga. At 0.7 M NaCl, the proportion of polyenoic acids grew less significantly, but biomass concentration and total nitrogen increased, similarly to the experiment with 0.3 M NaCl. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhila, N.O.; Kalacheva, G.S.; Volova, T.G.

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