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ANALYSIS OF THE COMPOSITION OF LIPIDS FROM CARBOXYDOBACTERIA [Text] / G. S. KALACHEVA, T. G. VOLOVA, I. N. TRUBACHEV // Microbiology. - 1980. - Vol. 49, Is. 5. - P. 595-600. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VOLOVA, T.G.; TRUBACHEV, I.N.

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ANALYSIS OF THE COMPOSITION OF LIPIDS FROM CARBOXYDOBACTERIA [Text] / G. S. KALACHEVA, T. G. VOLOVA, I. N. TRUBACHEV // Microbiology. - 1980. - Vol. 49, Is. 5. - P595-600. - Cited References: 17 . - 6. - ISSN 0026-2617

РУБ Microbiology

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VOLOVA, T.G.; TRUBACHEV, I.N.

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LIPIDS OF THE LUMINESCENT BACTERIUM PHOTOBACTERIUM-MANDAPAMENSIS [Text] / G. S. KALACHEVA [et al.] // Microbiology. - 1981. - Vol. 50, Is. 1. - P56-60. - Cited References: 15 . - 5. - ISSN 0026-2617

РУБ Microbiology

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VYSOTSKII, E.S.; RODICHEVA, E.K.; FISH, A.M.

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LIPIDS OF THE LUMINESCENT BACTERIUM PHOTOBACTERIUM-MANDAPAMENSIS [Text] / G. S. KALACHEVA [et al.] // Microbiology. - 1981. - Vol. 50, Is. 1. - P. 56-60. - Cited References: 15 . - ISSN 0026-2617

РУБ Microbiology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VYSOTSKII, E.S.; RODICHEVA, E.K.; FISH, A.M.

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LIPIDS OF THE BLUE-GREEN-ALGA SYNECHOCOCCUS-ELONGATUS [Text] / G. S. KALACHEVA, I. N. TRUBACHEV // SOVIET PLANT PHYSIOLOGY. - 1981. - Vol. 28, Is. 3. - P. 366-370. - Cited References: 24 . - ISSN 0038-5719

РУБ Plant Sciences

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; TRUBACHEV, I.N.

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CHANGE IN LIPIDS AND THEIR FATTY-ACID COMPOSITION IN BATCH CULTURING OF LUMINESCENT BACTERIA [Text] / G. S. KALACHEVA, E. S. VYSOTSKII // Microbiology. - 1984. - Vol. 53, Is. 6. - P756-760. - Cited References: 16 . - 5. - ISSN 0026-2617

РУБ Microbiology

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VYSOTSKII, E.S.

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CHANGE IN LIPIDS AND THEIR FATTY-ACID COMPOSITION IN BATCH CULTURING OF LUMINESCENT BACTERIA [Text] / G. S. KALACHEVA, E. S. VYSOTSKII // Microbiology. - 1984. - Vol. 53, Is. 6. - P. 756-760. - Cited References: 16 . - ISSN 0026-2617

РУБ Microbiology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KALACHEVA, G.S.; VYSOTSKII, E.S.

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Changes of lipids and their fatty acid composition during batch cultivation of the luminescent bacterium / G. S. Kalacheva, E. S. Vysotsky // Mikrobiologiya. - 1984. - Vol. 53, Is. 6. - С. 932-937 . - ISSN 0026-3656
Кл.слова (ненормированные):
fatty acid -- lipid -- bacterium -- bioluminescence -- nonhuman

Scopus
Держатели документа:
Institut Biofiziki, SO AN SSSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Vysotsky, E.S.

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10.

Physiological and biochemical properties of the alga Botryococcus braunii / T. G. Volova [et al.] // Russian Journal of Plant Physiology. - 1998. - Vol. 45, Is. 6. - P775-779 . - ISSN 1021-4437
Кл.слова (ненормированные):
Batch culture -- Botryococcus braunii -- Liquid hydrocarbons -- Productivity
Аннотация: The growth, productivity, and changes in the chemical composition of the green alga Botryococcus braunii Kutz H-252 were studied at different phases of growth in a luminostat culture. Modification of the Prat medium resulted in an increase in the alga yield to 3.9 g/l and a decrease in the generation time to 3-4 days. The specific growth rate during the exponential phase was 0.235/ day. These values are comparable with the best results obtained abroad. Among the pigments of B. braunii, besides chlorophylls a and b, carotene, lutein, neoxanthin, and violaxanthin were also identified. The highest content of these pigments, based on dry weight, was achieved during the exponential growth phase. During culture aging, there was an accumulation of carbohydrates and lipids in addition to a simultaneous decrease in the concentration of nitrogen-containing substances. It was discovered that this strain can synthesize liquid hydrocarbons. These hydrocarbons were found both in the alga biomass (ca. 1% of dry weight) and among the extracellular metabolites (up to 10.3 mg/l).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Zhilo, N.O.; Plotnikov, V.F.

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11.

Characteristics of lipids from green alga Botryococcus braunii synthesizing liquid hydrocarbons. / T. G. Volova, G. S. Kalacheva, N. O. Zhyla // Doklady Biological Sciences. - 2000. - Vol. 370, Is. 1-6. - P96-99 . - ISSN 0012-4966
Кл.слова (ненормированные):
fatty acid -- hydrocarbon -- lipid -- article -- biomass -- chemistry -- green alga -- metabolism -- Algae, Green -- Biomass -- Fatty Acids -- Hydrocarbons -- Lipids

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Zhyla, N.O.

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12.

Nature of lipids of algae Botryococcus braunii synthesis liquids hydrocarbons [Текст] / T. G. Volova, G. S. Kalacheva, N. O. Zhila // Dokl. Akad. Nauk. - 2000. - Vol. 370, Is. 6. - С. 838-841. - Cited References: 11 . - 4. - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
GROWTH

Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Zhila, N.O.

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13.

Lipids of the green alga Botryococcus cultured in a batch mode [Text] / G. S. Kalacheva, N. O. Zhila, T. G. Volova // Microbiology. - 2001. - Vol. 70, Is. 3. - P. 256-262, DOI 10.1023/A:1010438908047. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
BRAUNII
   HYDROCARBON
   METABOLISM
   RACE
Кл.слова (ненормированные):
Botryococcus braunii -- batch culture -- lipids -- fatty acids
Аннотация: The lipid fraction of the green alga Botryococcus cultured in a batch mode was found to contain polar lipids (more than 50% of the total lipids), di- and triacylglycerols, sterols and their eaters, free fatty acids, and hydrocarbons. In aging culture, the content of polar lipids somewhat decreased and that of triacylglycerols increased by more than four times. The content of hydrocarbons in the algal biomass did not exceed 0.9% and depended little on the culture age. Intracellular lipids contained saturated and unsaturated (mono-, di-, and trienoic) fatty acids. The maximum content of C-16:3 and alpha -C-18 :3 fatty acids (up to 35% of the total fatty acids) was detected in the phase of active growth. The extracellular and intracellular lipids of the al,oa differed in the proportion of particular lipids and in the fatty acid pattern.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.

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14.

Lipids of the green alga Botryococcus cultured in a batch mode / G. S. Kalacheva, N. O. Zhila, T. G. Volova // Microbiology. - 2001. - Vol. 70, Is. 3. - P256-262 . - ISSN 0026-2617
Кл.слова (ненормированные):
Batch culture -- Botryococcus braunii -- Fatty acids -- Lipids
Аннотация: The lipid fraction of the green alga Botryococcus cultured in a batch mode was found to contain polar lipids (more than 50% of the total lipids), di- and triacylglycerols, sterols and their esters, free fatty acids, and hydrocarbons. In aging culture, the content of polar lipids somewhat decreased and that of triacylglycerols increased by more than four times. The content of hydrocarbons in the algal biomass did not exceed 0.9% and depended little on the culture age. Intracellular lipids contained saturated and unsaturated (mono-, di-, and trienoic) fatty acids. The maximum content of C16:3 and ?-C18:3 fatty acids (up to 35% of the total fatty acids) was detected in the phase of active growth. The extracellular and intracellular lipids of the alga differed in the proportion of particular lipids and in the fatty acid pattern. В© 2001 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.

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15.

The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752 . - ISSN 0026-3656
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis -- alkane -- carbon monoxide -- fatty acid -- hydroxyacid -- lipid -- nitrogen -- polyester -- article -- chemistry -- culture medium -- drug antagonism -- growth, development and aging -- mass spectrometry -- metabolism -- Wautersia eutropha -- Alkanes -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Hydroxy Acids -- Lipids -- Mass Spectrometry -- Nitrogen -- Polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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16.

The effect of temperature on the lipid composition of Botryococcus / G. S. Kalacheva [и др.] // Mikrobiologiya. - 2002. - Vol. 71, Is. 3. - С. 336-344 . - ISSN 0026-3656
Кл.слова (ненормированные):
fatty acid -- lipid -- triacylglycerol -- article -- biosynthesis -- chemistry -- comparative study -- green alga -- growth, development and aging -- metabolism -- temperature -- Algae, Green -- Fatty Acids -- Lipids -- Temperature -- Triglycerides
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal, optimal, and supraoptimal. Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.; Gladyshev, M.I.

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17.

Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - P675-678, DOI 10.1007/s00253-002-0941-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
3 hydroxybutyric acid -- acetoacetyl coenzyme a reductase -- acetyl coenzyme A acyltransferase -- beta hydroxyvalerate -- butyrate dehydrogenase -- carbon monoxide -- electrolyte -- hydrogen -- oxidoreductase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid)synthase -- polyhydroxyalkanoic acid -- polymer -- unclassified drug -- valeric acid -- bacterium -- article -- autotrophy -- bacterial growth -- bacterial strain -- biomass production -- controlled study -- crystallization -- enzyme activity -- molecular weight -- nonhuman -- synthesis -- temperature -- Wautersia eutropha -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Lipids -- Polyesters -- Bacteria (microorganisms) -- Negibacteria -- Ralstonia -- Wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. Russian Academy of Sci., 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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18.

The effect of temperature on the lipid composition of the green alga Botryococcus / G. S. Kalacheva [et al.] // Microbiology. - 2002. - Vol. 71, Is. 3. - P286-293, DOI 10.1023/A:1015898426573 . - ISSN 0026-2617
Кл.слова (ненормированные):
Fatty acids -- Green alga -- Lipids -- Temperature -- algae -- Botryococcus -- Chlorophyta
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal (18В°C), optimal (25В°C), and supraoptimal (32В°C). Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature. В© 2002 MAIK "Nauka/ Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.; Gladyshev, M.I.

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19.

The effect of temperature on the lipid composition of the green alga Botryococcus [Text] / G. S. Kalacheva [et al.] // Microbiology. - 2002. - Vol. 71, Is. 3. - P. 286-293, DOI 10.1023/A:1015898426573. - Cited References: 14 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
METABOLISM
CULTURES
BRAUNII
Кл.слова (ненормированные):
green alga -- lipids -- fatty acids -- temperature
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal (18degreesC), optimal (25degreesC), and supraoptimal (32degreesC). Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.; Gladyshev, M.I.

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20.

Lipid and hydrocarbon compositions of a collection strain and a wild sample of the green microalga Botryococcus / G. S. Kalacheva, N. O. Zhila, T. G. Volova // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P317-330, DOI 10.1023/A:1015615618420 . - ISSN 1386-2588
Кл.слова (ненормированные):
Batch culture -- Botoryococcus braunii -- Fatty acids -- Hydrocarbons -- Lipids -- chemical composition -- fatty acid -- green alga -- hydrocarbon -- lipid -- saline lake -- Russian Federation -- algae -- Bacteria (microorganisms) -- Botryococcus -- Botryococcus braunii -- Chlorophyta
Аннотация: Lipid composition and hydrocarbon structure of two colonial green algae of the genus Botryococcus, i.e., a mu seum strain and a field sample collected for the first time from Lake Shira (Khakasia, Siberia), have been compared. Polar lipids, diacylglycerols, alcohols, triacylglycerols, sterols, sterol esters, free fatty acids and hydrocarbons have been identified among lipids in the laboratory culture. The dominant fraction in the museum strain was formed by polar lipids (up to 50% of the lipids) made up of fatty acids from C12 to C24. Palmitic, oleic, C16 - C18 dienoic and trienoic acids were the main fatty acids of the museum strain. Aliphatic hydrocarbons were found in the lipid of the museum strain. However, these amounted maximally to about 1% of the dry biomass at the end of exponential growth phase. The qualitative and quantitative compositions of FAs and hydrocarbons of the museum strain of Botryococcus, (registered at the Cambridge collection as Botryococcus braunii Kutz No LB 807/1 Droop 1950 H-252) differed from those of the Botryococcus strain described in the literature as Botryococcus braunii. The Botryococcus sp. found in Lake Shira is characterized by a higher lipid content (< 40% of the dry weight). Polar lipids, sterols, triacylglycerols, free fatty acids and hydrocarbons have been identified among lipids in the field sample. The main lipids in this sample were dienes and trienes (hydrocarbons < 60% of total lipid). Monounsaturated and very long chain monounsaturated fatty acids, including C28:1 and C32:1 acids, were identified in the Botryococcus found in Lake Shira. The chemo-taxonomic criteria allow us to unequivocally characterize the organism collected from Lake Shira as Botryococcus braunii race A.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.

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