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Иностранные журналы библиотеки Института биофизики СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wu Y. -T., Chiang P. -W., Tandon K., Rogozin D. Y., Degermendzhy A. G., Tang S. -L.
Заглавие : Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia
Место публикации : Microb. Genomics: Microbiology Society, 2021. - Vol. 7, Is. 12. - Ст.000712. - ISSN 20575858 (ISSN), DOI 10.1099/mgen.0.000712
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mitiouchkina, Tatiana, Mishin, Alexander S., Somermeyer, Louisa Gonzalez, Markina, Nadezhda M., Chepurnyh, Tatiana, V, Guglya, Elena B., Karataeva, Tatiana A., Palkina, Kseniia A., Shakhova, Ekaterina S., Fakhranurova, Liliia, I, Chekova, Sofia, V, Tsarkova, Aleksandra S., Golubev, Yaroslav, V, Negrebetsky, Vadim V., Dolgushin, Sergey A., Shalaev, Pavel, V, Shlykov, Dmitry, Melnik, Olesya A., Shipunova, Victoria O., Deyev, Sergey M., Bubyrev, Andrey, I, Pushin, Alexander S., Choob, Vladimir V., Dolgov, Sergey, V, Kondrashov, Fyodor A., Yampolsky, Ilia, V, Sarkisyan, Karen S.
Заглавие : Plants with genetically encoded autoluminescence
Колич.характеристики :7 с
Коллективы : Planta LLC; Skolkovo Innovation Centre; MRC London Institute of Medical Sciences [UKRI MC-A658-5QEA0]; Imperial College Research Fellowship; Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility (CKP IBCH - Russian Ministry of Education and Science Grant) [RFMEFI62117X0018]; ERC grant [771209-CharFL]; European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant [665385]; Russian Science Foundation grantRussian Science Foundation (RSF) [19-74-10102, 17-14-01169p, 16-14-00052p]; Ministry of Science and Higher Education of the Russian Federation [075-15-2019-1789]; [075-15-2019-411]
Место публикации : Nat. Biotechnol.: NATURE PUBLISHING GROUP, 2020. - Article in press. - ISSN 1087-0156, DOI 10.1038/s41587-020-0500-9. - ISSN 1546-1696(eISSN)
Примечания : Cited References:17. - This study was designed, performed and funded by Planta LLC. We thank K. Wood for assisting in manuscript development. Planta acknowledges support from the Skolkovo Innovation Centre. We thank D. Bolotin and the Milaboratory (milaboratory. com) for access to computing and storage infrastructure. We thank S. Shakhov for providing photography equipment. The Synthetic Biology Group is funded by the MRC London Institute of Medical Sciences (UKRI MC-A658-5QEA0, K.S.S.). K.S.S. is supported by an Imperial College Research Fellowship. Experiments were partially carried out using equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility (CKP IBCH; supported by the Russian Ministry of Education and Science Grant RFMEFI62117X0018). The F.A.K. lab is supported by ERC grant agreement 771209-CharFL. This project received funding from the European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant Agreement 665385. K.S.S. acknowledges support by President's Grant 075-15-2019-411. Design and assembly of some of the plasmids was supported by Russian Science Foundation grant 19-74-10102. Imaging experiments were partially supported by Russian Science Foundation grant 17-14-01169p. LC-MS/MS analyses of extracts were supported by Russian Science Foundation grant 16-14-00052p. Design and assembly of plasmids was partially supported by grant 075-15-2019-1789 from the Ministry of Science and Higher Education of the Russian Federation allocated to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine.
Предметные рубрики: METABOLISM
LIBRARY
Аннотация: Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in plastids have not been widely adopted because of low light output. We engineered tobacco plants with a fungal bioluminescence system that converts caffeic acid (present in all plants) into luciferin and report self-sustained luminescence that is visible to the naked eye. Our findings could underpin development of a suite of imaging tools for plants.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrov, Klim A., Dudareva, Lyubov, V, Nokhsorov, Vasiliy V., Stoyanov, Kirill N., Makhutova, Olesia N.
Заглавие : Fatty Acid Content and Composition of the Yakutian Horses and Their Main Food Source: Living in Extreme Winter Conditions
Колич.характеристики :15 с
Коллективы : Russian FederationRussian Federation [51.1.1]; Ministry of Science and Higher Education of the Russian Federation; Ministry of Education and Science of RussiaMinistry of Education and Science, Russian Federation [FSRG-2020-0019]
Место публикации : Biomolecules: MDPI, 2020. - Vol. 10, Is. 2. - Ст.315. - ISSN 2218-273X(eISSN), DOI 10.3390/biom10020315
Примечания : Cited References:62. - This research was funded by the State Assignment within the framework of the fundamental research program of the Russian Federation, topic No. 51.1.1; the Government Assignment given by the Ministry of Science and Higher Education of the Russian Federation to Siberian Federal University in 2020 (Project "Biologically active substances in trophic chains of aquatic ecosystems as essential components of human diet and markers for fisheries resource conservation"; the state task of the Ministry of Education and Science of Russia (FSRG-2020-0019).
Предметные рубрики: MEMBRANE-LIPIDS
MEAT QUALITY
TEMPERATURE
CARCASS
GROWTH
Аннотация: For the first time, seasonal changes in the content of total lipids (TLs) and phospholipids (PLs) were studied in fodder plants growing in Central Yakutia-a perennial cereal, smooth brome (Bromopsis inermis L.), and an annual cereal, common oat (Avena sativa L.). Both species have concentrated TLs and PLs in autumn under cold hardening. In addition, a significant increase in the content of fatty acids (FAs) of B. inermis was observed during the autumn decrease in temperature. The Yakutian horses, which fed on cereals enriched with nutrients preserved by natural cold (green cryo-fodder), accumulated significant amounts of 18:2n-6 and 18:3n-3, the total content of which in cereals was 75% of the total FA content. We found differences in the distribution of these two FAs in different tissues of the horses. Thus, liver was rich in 18:2n-6, while muscle and adipose tissues accumulated mainly 18:3n-3. Such a distribution may indicate different roles of these FAs in the metabolism of the horses. According to FA content, meat of the Yakutian horses is a valuable dietary product.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrov K. A., Dudareva L. V., Nokhsorov V. V., Stoyanov K. N., Makhutova O. N.
Заглавие : Fatty acid content and composition of the yakutian horses and their main food source: living in extreme winter conditions
Место публикации : Biomolecules: MDPI AG, 2020. - Vol. 10, Is. 2. - Ст.315. - ISSN 2218273X (ISSN), DOI 10.3390/biom10020315
Аннотация: For the first time, seasonal changes in the content of total lipids (TLs) and phospholipids (PLs) were studied in fodder plants growing in Central Yakutia—a perennial cereal, smooth brome (Bromopsis inermis L.), and an annual cereal, common oat (Avena sativa L.). Both species have concentrated TLs and PLs in autumn under cold hardening. In addition, a significant increase in the content of fatty acids (FAs) of B. inermis was observed during the autumn decrease in temperature. The Yakutian horses, which fed on cereals enriched with nutrients preserved by natural cold (green cryo-fodder), accumulated significant amounts of 18:2n-6 and 18:3n-3, the total content of which in cereals was 75% of the total FA content. We found differences in the distribution of these two FAs in different tissues of the horses. Thus, liver was rich in 18:2n-6, while muscle and adipose tissues accumulated mainly 18:3n-3. Such a distribution may indicate different roles of these FAs in the metabolism of the horses. According to FA content, meat of the Yakutian horses is a valuable dietary product. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrov K. A., Makhutova O. N., Gladyshev M. I.
Заглавие : Fatty Acid Composition of Yakut Horse Tissues
Место публикации : Doklad. Biochem. Biophys.: Pleiades Publishing, 2020. - Vol. 492, Is. 1. - С. 105-107. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672920030047
Аннотация: Abstract: We compared the composition and content of fatty acids (FAs) in the liver, muscles, and subcutaneous fat of Yakut horses inhabiting extreme environment in the Cryolithozone. Essential linoleic and alpha-linolenic acids, supplied to horses with their food, were accumulated in different tissues. Linoleic acid was accumulated in the liver but alpha-linolenic acid was accumulated in muscle and subcutaneous fat. Such a distribution indicates different roles of these fatty acids in the metabolism of horses. Yakut horse meat is a valuable dietary product owing to its fatty acid composition and content. © 2020, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrov K. A., Makhutova O. N., Gladyshev M. I.
Заглавие : Fatty Acid Composition of Yakut Horse Tissues
Колич.характеристики :3 с
Коллективы : Russian FederationRussian Federation [51.1.1]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 492, Is. 1. - С. 105-107. - ISSN 1607-6729, DOI 10.1134/S1607672920030047. - ISSN 1608-3091(eISSN)
Примечания : Cited References:8. - This study was supported by the state assignment within the framework of the Basic Research Program of the Russian Federation (topic no. 51.1.1) and the state assignment of the Ministry of Science and Higher Education of the Russian Federation to Siberian Federal University in 2020 (Project no. FSRZ-2020-0006 "Biologically active substances in environmental, biotechnological and medical systems").
Предметные рубрики: MEAT
Аннотация: We compared the composition and content of fatty acids (FAs) in the liver, muscles, and subcutaneous fat of Yakut horses inhabiting extreme environment in the Cryolithozone. Essential linoleic and alpha-linolenic acids, supplied to horses with their food, were accumulated in different tissues. Linoleic acid was accumulated in the liver but alpha-linolenic acid was accumulated in muscle and subcutaneous fat. Such a distribution indicates different roles of these fatty acids in the metabolism of horses. Yakut horse meat is a valuable dietary product owing to its fatty acid composition and content.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi M. A., Ivanov I. A., Rodionova N. S., Kovalchuk S. I., Kaskova Z. M., Petushkov V. N.
Заглавие : α-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp.
Место публикации : ChemistrySelect: Wiley-Blackwell, 2020. - Vol. 5, Is. 42. - С. 13155-13159. - ISSN 23656549 (ISSN), DOI 10.1002/slct.202003075
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as ?-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp. © 2020 Wiley-VCH GmbH
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi, Maxim A., Ivanov, Igor A., Rodionova, Natalia S., Kovalchuk, Sergey I., Kaskova, Zinaida M., Petushkov, Valentin N.
Заглавие : alpha-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp.
Колич.характеристики :5 с
Коллективы : State Assignment for Basic Research of the Russian Academy of Sciences [0356-2019-0019]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00348-a]
Место публикации : ChemistrySelect: WILEY-V C H VERLAG GMBH, 2020. - Vol. 5, Is. 42. - С. 13155-13159. - ISSN 2365-6549, DOI 10.1002/slct.202003075
Примечания : Cited References:49. - This work was supported by the State Assignment for Basic Research of the Russian Academy of Sciences (project no. 0356-2019-0019) and the Russian Foundation for Basic Research (project no. 19-04-00348-a).
Предметные рубрики: STRUCTURE ELUCIDATION
MANNOSYLATION
TRYPTOPHAN
PROTEIN
COMPLEMENT
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as alpha-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Derevtsova S. N., Romanenko A. A., Kolenchukova O. A., Stepanova L. V., Nikolaev V. G., Sindeeva L. V., Kratasyuk V. A., Medvedevа N. N.
Заглавие : Indicators of chemiluminescent and bioluminescent tests of biological liquids in the assessment of physical health
Место публикации : Klin. Lab. Diagn.: Klinichescheskaya Laboratornaya Diagnostika, 2020. - Vol. 65, Is. 9. - С. 541-546. - ISSN 08692084 (ISSN), DOI 10.18821/0869-2084-2020-65-8-541-546
Аннотация: The study includes anthropometry of 172 young male, obtained data on the length and body mass, measured the transverse diameters of the shoulders and pelvis, various body types was identified by the J.M. Tanner sexual dimorphism index (andromorphic, mesomorphic, gynecomorphic). The chemiluminescent and bioluminescent study of saliva and blood was conducted in the examined young male. We studied the indicators of the antioxidant defense system under the influence of stress. The antioxidant status of saliva was determined using the H2 O2-luminol-dependent chemiluminescence method. Data on the activity of NAD (P)-dependent dehydrogenases in blood lymphocytes was obtained from a bioluminescent method of research. Young male of andromorphic body type had large overall and transverse body sizes. Indicators of antioxidant protection of saliva and blood in men of adolescence, the body type of the sexual dimorphism index J.M. Tanner was different. The persons of the andromorphic body type differed in terms of chemiluminescence in comparison with the young male of gynecomorphic body type. The results of bioluminescent blood tests suggest a violation of the catabolic and anabolic processes of carbohydrate and fat metabolism in young men of mesomorphic and gynecomorphic body types. Indicators of the system of antioxidant protection of saliva and blood reflect the sexual characteristics of the body of young male and can be used as additional criteria for diagnosing sex inversion and assessing the risk of developing socially attributed diseases. © 2020, Klinichescheskaya Laboratornaya Diagnostika. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pande S., Ranjan R., Kratasyuk V. A.
Заглавие : Is Body Mass Index a potential biomarker for anemia in obese adolescents?
Место публикации : J. Nutr. Intermediary Metab.: Elsevier Inc, 2019. - Vol. 15. - С. 1-2. - ISSN 23523859 (ISSN) , DOI 10.1016/j.jnim.2018.11.001
Ключевые слова (''Своб.индексиров.''): anemia--body mass index--hepcidin--leptin--obesity
Аннотация: The two paradoxical major health problems namely obesity and anemia are confirmed to affect millions around the world. Hepcidin, a protein synthesized in liver is a negative iron binding regulator. There is an affirmative relation between hepcidin and leptin levels and an inverse co-relation between hepcidin and iron status due to inflammation mediated by obesity in adolescents. So this implicates an alliance between anemia and obesity wherein weight reduction can be a powerful medium to improve iron absorption in obese adolescents. Also the Body Mass Index can serve as a preliminary non-invasive screening tool to identify potential adolescents prone to anemia. © 2018
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov, Alexander A., Ushakova, Sofya A., Velichko, Vladimir V., Trifonov, Sergey, V, Tikhomirova, Natalia A., Kalacheva, Galina S.
Заглавие : A small closed ecosystem with an estimated portion of human metabolism
Колич.характеристики :5 с
Коллективы : Russian Science Foundation [14-14-00599]
Место публикации : Life Sci. Space Res.: ELSEVIER SCIENCE BV, 2018. - Vol. 19. - С. 63-67. - ISSN 2214-5524, DOI 10.1016/j.lssr.2018.10.001. - ISSN 2214-5532(eISSN)
Примечания : Cited References:11. - The study was supported by the Russian Science Foundation (Project No. 14-14-00599.) and carried out in the IBP SB RAS at FRC KRC SB RAS.
Ключевые слова (''Своб.индексиров.''): closed ecosystem--physicochemical waste processing--biochemical waste--processing--cycling process
Аннотация: The study describes a small closed ecosystem used to test technologies to be further employed in full-scale manned closed ecosystems. The experimental ecosystem is designed to use a certain portion of human metabolism, which is included in the gas, water, and organic waste loops of the system. In this experimental ecosystem, gas and water loops are fully closed, and the model enables processing of human waste and plant inedible biomass. A physicochemical method is used to remove pollutants from the air in the system. A human takes part in the gas exchange of the system through its respiration loop. This experimental ecosystem can be used for testing and improving new technologies to be further used in the future space stations.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vereshchagina, Kseniya, Kondrateva, Elizaveta, Axenov-Gribanov, Denis, Shatilina, Zhanna, Khomich, Andrey, Bedulina, Daria, Zadereev, Egor, Timofeyev, Maxim
Заглавие : Nonspecific stress response to temperature increase in Gammarus lacustris Sars with respect to oxygen-limited thermal tolerance concept
Колич.характеристики :17 с
Коллективы : Russian Science Foundation [17-14-01063]; Russian Foundation for Basic Research [16-34-00687, 16-34-60060, 17-34-50012]; Goszadanie project joint program of DAAD [6.9654.2017/8.9]; Ministry of education and Science M. Lomonosov [6.12735.2018/12.2]; Lake Baikal Foundation [FOB_02-3/05]
Место публикации : PeerJ: PEERJ INC, 2018. - Vol. 6. - Ст.e5571. - ISSN 2167-8359, DOI 10.7717/peerj.5571
Примечания : Cited References:49. - The study was carried out with the main financial support of Russian Science Foundation grant 17-14-01063, with the partial financial support of Russian Foundation for Basic Research grants 16-34-00687, 16-34-60060, 17-34-50012, the base part of Goszadanie project 6.9654.2017/8.9, joint program of DAAD and Ministry of education and Science M. Lomonosov (6.12735.2018/12.2) and Lake Baikal Foundation (FOB_02-3/05). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Предметные рубрики: COPEPOD TIGRIOPUS-JAPONICUS
SHUNET SOUTH SIBERIA
HEAT-SHOCK PROTEINS
Ключевые слова (''Своб.индексиров.''): gammarus lacustris--heat shock proteins 70 (hsp70)--nonspecific cellular--stress-response (ncsr)--lactate dehydrogenase--diene conjugates--schiff--bases--triene conjugates
Аннотация: The previously undescribed dynamics of the heat shock protein HSP70 and subsequent lipid peroxidation products have been assessed alongside lactate dehydrogenase activity for Gammarus lacustris Sars, an amphipod species from the saltwater Lake Shira (Republic of Khakassia). Individuals were exposed to a gradual temperature increase of 1 degrees C/hour (total exposure duration of 26 hours) starting from the mean annual temperature of their habitat (7 degrees C) up to 33 degrees C. A complex of biochemical reactions occurred when saltwater G. lactustris was exposed to the gradual changes in temperature. This was characterized by a decrease in lactate dehydrogenase activity and the launching of lipid peroxidation. The HSP70 level did not change significantly during the entire experiment. In agreement with the concept of oxygen-limited thermal tolerance, an accumulation of the most toxic lipid peroxides (triene conjugates and Schiff bases) in phospholipids occurred at the same time and temperature as the accumulation of lactate. The main criterion overriding the temperature threshold was, therefore, the transition to anaerobiosis, confirmed by the elevated lactate levels as observed in our previous associated study, and by the development of cellular stress, which was expressed by an accumulation of lipid peroxidation products. An earlier hypothesis, based on freshwater individuals of the same species, has been confirmed whereby the increased thermotolerance of G. lacustris from the saltwater lake was caused by differences in energy metabolism and energy supply of nonspecific cellular stress-response mechanisms. With the development of global climate change, these reactions could be advantageous for saltwater G. lacustris. The studied biochemical reactions can be used as biomarkers for the stress status of aquatic organisms when their habitat temperature changes.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wu, Yu-Ting, Yang, Cheng-Yu, Chiang, Pei-Wen, Tseng, Ching-Hung, Chiu, Hsiu-Hui, Saeed, Isaam, Baatar, Bayanmunkh, Rogozin, Denis, Halgamuge, Saman, Degermendzhi, Andrei, Tang, Sen-Lin
Заглавие : Comprehensive Insights Into Composition, Metabolic Potentials, and Interactions Among Archaeal, Bacterial, and Viral Assemblages in Meromictic Lake Shunet in Siberia
Колич.характеристики :21 с
Коллективы : Ministry of Science and Technology in Taiwan [NSC 99-2923-B-001-001-MY3, NSC 102-2923-B-001-004]; Russian Foundation for Basic Research [16-05-00091-a]; Russian Federation [NSh-9249.2016.5]
Место публикации : Front. Microbiol.: FRONTIERS MEDIA SA, 2018. - Vol. 9. - Ст.1763. - ISSN 1664-302X, DOI 10.3389/fmicb.2018.01763
Примечания : Cited References:103. - This research was supported by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC 99-2923-B-001-001-MY3 and NSC 102-2923-B-001-004 and the Russian Foundation for Basic Research, Grant No. 16-05-00091-a. The research was partially supported by the Council on grants from the President of the Russian Federation for support of leading scientific schools (grant NSh-9249.2016.5).
Предметные рубрики: BACTERIOPLANKTON COMMUNITY COMPOSITION
RIBOSOMAL-RNA GENES
ANTARCTIC
Ключевые слова (''Своб.индексиров.''): lake shunet--metagenomics--archaeal--bacterial and viral assemblages--meromictic lake
Аннотация: Microorganisms are critical to maintaining stratified biogeochemical characteristics in meromictic lakes; however, their community composition and potential roles in nutrient cycling are not thoroughly described. Both metagenomics and metaviromics were used to determine the composition and capacity of archaea, bacteria, and viruses along the water column in the landlocked meromictic Lake Shunet in Siberia. Deep sequencing of 265 Gb and high-quality assembly revealed a near-complete genome corresponding to Nonlabens sp. sh3vir. in a viral sample and 38 bacterial bins (0.2-5.3 Mb each). The mixolimnion (3.0 m) had the most diverse archaeal, bacterial, and viral communities, followed by the monimolimnion (5.5 m) and chemocline (5.0 m). The bacterial and archaeal communities were dominated by Thiocapsa and Methanococcoides, respectively, whereas the viral community was dominated by Siphoviridae. The archaeal and bacterial assemblages and the associated energy metabolism were significantly related to the various depths, in accordance with the stratification of physicochemical parameters. Reconstructed elemental nutrient cycles of the three layers were interconnected, including co-occurrence of denitrification and nitrogen fixation in each layer and involved unique processes due to specific biogeochemical properties at the respective depths. According to the gene annotation, several pre-dominant yet unknown and uncultured bacteria also play potentially important roles in nutrient cycling. Reciprocal BLAST analysis revealed that the viruses were specific to the host archaea and bacteria in the mixolimnion. This study provides insights into the bacterial, archaeal, and viral assemblages and the corresponding capacity potentials in Lake Shunet, one of the three meromictic lakes in central Asia. Lake Shunet was determined to harbor specific and diverse viral, bacterial, and archaeal communities that intimately interacted, revealing patterns shaped by indigenous physicochemical parameters.
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15.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Yu Saltykov M., Bartsev S. I.
Заглавие : Developing of discrimination experiment to find most adequate model of plant's multi-nutrient functional response
Место публикации : IOP Conference Series: Materials Science and Engineering: Institute of Physics Publishing, 2017. - Vol. 173: 5th International Workshop on Mathematical Models and their Applications 2016, IWMMA 2016 (7 November 2016 through 9 November 2016, ) Conference code: 126825, Is. 1. - , DOI 10.1088/1757-899X/173/1/012017
Ключевые слова (''Своб.индексиров.''): ecology--metabolism--physiology--adequate models--complex model--discrimination experiments--ecological models--functional response--trophic level--typical patterns--nutrients
Аннотация: To create reliable Closed Ecological Life Support Systems (CELSS) it is necessary to have models which can predict CELSS dynamic with good accuracy. However it was shown that conventional ecological models cannot describe CELSS correctly if it is closed by more than one element. This problem can be solved by means more complex models than conventional ones - so called flexible metabolism models. However it is possible that CELSS also can be described correctly in "semi-conventional" framework - when only one trophic level is described by flexible metabolism model. Another problem in CELSS modeling is existence of different and incompatible hypotheses about relationships between plants growth rate and amounts of nutrients (functional responses). Difficulty of testing these hypotheses is associated with multi-nutrient dependency of growth rate and comprehensive experimental studies are expensive and time-consuming. This work is devoted to testing the hypothesis that "semi-conventional" approach is enough to describe CELSS, and to planning the discrimination experiment on selecting correct type of the plant's functional response. To do that three different models of plants (one flexible and two conventional) were investigated both in the scope of CELSS model, and in hemostat model. Numerical simulations show that each of the models has typical patterns which can be determined in experiment with real plants. © Published under licence by IOP Publishing Ltd.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova E. N., Asanova A. A., Deeva A. A., Kratasyuk V. A.
Заглавие : Inhibition effect of food preservatives on endoproteinases
Место публикации : Food Chem.: Elsevier Ltd, 2017. - Vol. 235. - С. 294-297. - ISSN 03088146 (ISSN) , DOI 10.1016/j.foodchem.2017.05.059
Ключевые слова (''Своб.индексиров.''): endoproteinases--food additives--pancreatic disease--pancreatic enzymes--benzoic acid--enzyme activity--enzymes--food additives--food preservatives--potassium sorbate--sodium--acceptable daily intakes--decay constants--endoproteinases--human metabolisms--inhibition effect--light intensity--protein digestion--sodium benzoate--sorbic acid
Аннотация: The present manuscript proposes a novel approach to assess the impact of food additives on human metabolism by analysing their effect on biomarker enzyme activity. Alterations in the activity of pancreatic enzymes, such as chymotrypsin and trypsin, which are affected by the most common food preservatives, sodium benzoate (E211), potassium sorbate (E202) and sorbic acid (E200), have been evaluated. The proteinase activity was analysed with a bioluminescent method using the light intensity decay constant. Our study revealed that the preservatives reduce proteinase activity by 50% (EC50) at a much lower concentration than their acceptable daily intake (ADI). Thus, sodium benzoate and sorbic acid have an inhibition effect on chymotrypsin at concentrations 14 times lower and 70 times lower than their ADI and this increases with exposure time. Food preservative consumption impacts negatively on protein digestion, which is especially dangerous for patients with pancreatitis. © 2017 Elsevier Ltd
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vereshchagina K. P., Lubyaga Y. A., Shatilina Z., Bedulina D., Gurkov A., Axenov-Gribanov D. V., Baduev B., Kondrateva E. S., Gubanov M., Zadereev E., Sokolova I., Timofeyev M.
Заглавие : Salinity modulates thermotolerance, energy metabolism and stress response in amphipods Gammarus lacustris
Место публикации : PeerJ: PeerJ Inc., 2016. - Vol. 2016, Is. 11. - ISSN 21678359 (ISSN) , DOI 10.7717/peerj.2657
Ключевые слова (''Своб.индексиров.''): adaptation--amphipoda--gammarus lacustris--salinity--thermal tolerance
Аннотация: Temperature and salinity are important abiotic factors for aquatic invertebrates. We investigated the influence of different salinity regimes on thermotolerance, energy metabolism and cellular stress defense mechanisms in amphipods Gammarus lacustris Sars from two populations. We exposed amphipods to different thermal scenarios and determined their survival as well as activity of major antioxidant enzymes (peroxidase, catalase, glutathione S-transferase) and parameters of energy metabolism (content of glucose, glycogen, ATP, ADP, AMP and lactate). Amphipods from a freshwater population were more sensitive to the thermal challenge, showing higher mortality during acute and gradual temperature change compared to their counterparts from a saline lake. A more thermotolerant population from a saline lake had high activity of antioxidant enzymes. The energy limitations of the freshwater population (indicated by low baseline glucose levels, downward shift of the critical temperature of aerobic metabolism and inability to maintain steady-state ATP levels during warming) was ob- served, possibly reflecting a trade-off between the energy demands for osmoregulation under the hypo-osmotic condition of a freshwater environment and protection against temperature stress. © 2016 Vereshchagina et al.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova, Eugenia E., Krasitskaya, Vasilisa V., Frank, Ludmila A.
Заглавие : Simultaneous Genotyping of Four Single Nucleotide Polymorphisms Associated with Risk Factors of Hemostasis Disorders
Колич.характеристики :7 с
Коллективы : Russian Science Foundation [14-14-01119]
Место публикации : Comb. Chem. High Throughput Screen: BENTHAM SCIENCE PUBL LTD, 2015. - Vol. 18, Is. 10. - С. 930-936. - ISSN 1386-2073, DOI 10.2174/1386207318666150917095903. - ISSN 1875-5402(eISSN)
Примечания : Cited References:20. - The study was supported by the grant 14-14-01119 of the Russian Science Foundation.
Предметные рубрики: ALLELE-SPECIFIC PCR
FACTOR-V-LEIDEN
BIOLUMINESCENT IMMUNOASSAY
Ключевые слова (''Своб.индексиров.''): snp detection--pext reaction--photoprotein obelin--bioluminescent--microassay--multiplex pcr
Аннотация: Multiplex simultaneous genotyping technique was developed for four polymorphisms in genes coding for blood coagulation factors and homocysteine metabolism which are considered as thrombophilia related mutations: FV Leiden, FII G20210A, MTHFR C677T, and FVII G10976A. It is based on primer extension reaction with the following bioluminescent solid-phase microassay. At that, two different in bioluminescence obelin mutants were applied to simultaneous detection of two gene allelic variants. The assay is carried out in microtiter plate format and provides fast and reliable genotyping of four single nucleotide polymorphisms in four different genes within 2.5 hours. A large number of clinical samples were analyzed and the obtained results were found to be in complete correlation with those obtained by using conventional RT-PCR techniques.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova E. E., Krasitskaya V. V., Frank L. A.
Заглавие : Simultaneous genotyping of four single nucleotide polymorphisms associated with risk factors of hemostasis disorders
Место публикации : Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - С. 930-936. - ISSN 13862073 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescent microassay--multiplex pcr--pext reaction--photoprotein obelin--snp detection
Аннотация: Multiplex simultaneous genotyping technique was developed for four polymorphisms in genes coding for blood coagulation factors and homocysteine metabolism which are considered as thrombophilia related mutations: FV Leiden, FII G20210A, MTHFR C677T, and FVII G10976A. It is based on primer extension reaction with the following bioluminescent solid-phase microassay. At that, two different in bioluminescence obelin mutants were applied to simultaneous detection of two gene allelic variants. The assay is carried out in microtiter plate format and provides fast and reliable genotyping of four single nucleotide polymorphisms in four different genes within 2.5 hours. A large number of clinical samples were analyzed and the obtained results were found to be in complete correlation with those obtained by using conventional RT-PCR techniques. © 2015 Bentham Science Publishers.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shklavtsova E.S., Ushakova S.A., Shikhov V.N., Anishchenko O.V.
Заглавие : Effects of mineral nutrition conditions on heat tolerance of chufa (Cyperus esculentus L.) plant communities to super optimal air temperatures in the BTLSS
Место публикации : Adv. Space Res. - 2014. - Vol. 54, Is. 6. - С. 1135-1145. - ISSN 18791948 (ISSN) , DOI 10.1016/j.asr.2014.05.031
Ключевые слова (''Своб.индексиров.''): bioregenerative life support system--chlorophyll fluorescence--cyperus esculentus l.--heat shock--lipid peroxidation--mineralized human wastes--atmospheric temperature--carbon dioxide--lipids--metabolism--minerals--nitrates--nitrogen--nutrition--plants (botany)--urea--wastes--bioregenerative life support systems--chlorophyll fluorescence--cyperus esculentus--heat-shock--human waste--lipid peroxidation--plant shutdowns
Аннотация: The use of mineralized human wastes as a basis for nutrient solutions will increase the degree of material closure of bio-technical human life support systems. As stress tolerance of plants is determined, among other factors, by the conditions under which they have been grown before exposure to a stressor, the purpose of the study is to investigate the level of tolerance of chufa (Cyperus esculentus L.) plant communities grown in solutions based on mineralized human wastes to a damaging air temperature, 45 °C. Experiments were performed with 30-day-old chufa plant communities grown hydroponically, on expanded clay aggregate, under artificial light, at 690 ?mol m-2 s-1 PAR and at a temperature of 25 °C. Plants were grown in Knop's solution and solutions based on human wastes mineralized according to Yu.A. Kudenko's method, which contained nitrogen either as ammonium and urea or as nitrates. The heat shock treatment lasted 20 h at 690 and 1150 ?mol m -2 s-1 PAR. Chufa heat tolerance was evaluated based on parameters of CO2 gas exchange, the state of its photosynthetic apparatus (PSA), and intensity of peroxidation of leaf lipids. Chufa plants grown in the solutions based on mineralized human wastes that contained ammonium and urea had lower heat tolerance than plants grown in standard mineral solutions. Heat tolerance of the plants grown in the solutions based on mineralized human wastes that mainly contained nitrate nitrogen was insignificantly different from the heat tolerance of the plants grown in standard mineral solutions. A PAR intensity increase from 690 ?mol m -2 s-1 to 1150 ?mol m-2 s-1 enhanced heat tolerance of chufa plant communities, irrespective of the conditions of mineral nutrition under which they had been grown. © 2014 COSPAR. Published by Elsevier Ltd. All rights reserved.
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