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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi M. A., Ivanov I. A., Rodionova N. S., Kovalchuk S. I., Kaskova Z. M., Petushkov V. N.
Заглавие : α-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp.
Место публикации : ChemistrySelect: Wiley-Blackwell, 2020. - Vol. 5, Is. 42. - С. 13155-13159. - ISSN 23656549 (ISSN), DOI 10.1002/slct.202003075
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as ?-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp. © 2020 Wiley-VCH GmbH
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Tikhomirov A.A., Parshina O.V., Ushakova S.A., Kalacheva G.S.
Заглавие : Volatile metabolites of higher plant crops as a photosynthesizing life support system component under temperature stress at different light intensities
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1781-1786. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00121-2
Ключевые слова (''Своб.индексиров.''): composition--crops--heat resistance--metabolites--photosynthesis--volatile metabolites--space research--biosphere--article--comparative study--gas--growth, development and aging--heat--indoor air pollution--light--metabolism--microclimate--photon--photosynthesis--physiology--radiation exposure--volatilization--wheat--air pollution, indoor--environment, controlled--gases--heat--life support systems--light--photons--photosynthesis--triticum--volatilization
Аннотация: The effect of elevated temperatures of 35 and 45В°C (at the intensities of photosynthetically active radiation 322, 690 and 1104 ?mol-m-2-s-1) on the photosynthesis, respiration, and qualitative and quantitative composition of the volatiles emitted by wheat (Triticum aestuvi L., cultivar 232) crops was investigated in growth chambers. Identification and quantification of more than 20 volatile compounds (terpenoids - ?-pinene, ?3 carene, limonene, benzene, ?-and transcaryophyllene, ?- and ?-terpinene, their derivatives, aromatic hydrocarbons, etc.) were conducted by gas chromatograph/mass spectrometry. Under light intensity of 1104 ?mol-m-2-s-1, heat resistance of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. The action of elevated temperatures brought about variations in the rate and direction of the synthesis of volatile metabolites. The emission of volatile compounds was the greatest under a reduced irradiation of 322 ?mol-m-2-s-1 and the smallest under 1104 ?mol-m-2-s-1 at 35В°C. During the repair period, the contents and proportions of volatile compounds were different from their initial values, too. The degree of disruption and the following recovery of the functional state depended on the light intensity during the exposure to elevated temperatures. The investigation of the atmosphere of the growth chamber without plants has revaled the substances that were definitely technogenic in origin: tetramethylurea, dimethylsulfide, dibutylsulfide, dibutylphthalate, and a number of components of furan and silane nature. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitel'son I.I., Tikhomirov A.A., Parshina O.V., Ushakova S.A., Kalacheva G.S.
Заглавие : Volatile metabolites and external CO2 exchange of wheat cenoses under optimal conditions and thermal stress
Место публикации : Applied Biochemistry and Microbiology. - 2002. - Vol. 38, Is. 1. - С. 78-82. - ISSN 00036838 (ISSN) , DOI 10.1023/A:1013212907872
Ключевые слова (''Своб.индексиров.''): carbon dioxide--volatile agent--article--biosynthesis--carbon dioxide transport--chemical composition--concentration (parameters)--controlled study--cultivar--metabolite--nonhuman--photosynthesis--photosynthetically active radiation--plant metabolism--qualitative analysis--quantitative analysis--stress--temperature sensitivity--thermal exposure--thermostability--wheat--rickettsia sp. par--triticum--triticum aestivum
Аннотация: The effects of elevated temperature (35 and 45В°C) on photosynthesis, respiration, and both the qualitative and quantitative compositions of volatile emissions (VE) of wheat (Triticum aestuvum L. cultivar 232) cenoses at light intensities of 70, 150, or 240 W/m2 of photosynthetically available radiation (PAR) were studied. At a PAR of 240 W/m2, the thermal stabilities of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. Elevated temperatures nonuniformly changed the rates and direction of VE syntheses. In this process, the highest increase in VE evolution was observed at 70 W/m2 and 35В°C; the lowest, at 240 W/m2. In addition, the concentrations and composition of VE during the repair period differed from the initial values.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Kudenko Y.A., Ushakova S.A., Tirranen L.S., Gribovskaya I.A., Gros J.-B., Lasseur C.
Заглавие : Use of human wastes oxidized to different degrees in cultivation of higher plants on the soil-like substrate intended for closed ecosystems
Место публикации : Advances in Space Research. - 2010. - Vol. 46, Is. 6. - С. 744-750. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2010.02.024
Ключевые слова (''Своб.индексиров.''): life support systems--microflora--mineralized human wastes--phototrophic unit--wet incineration--alternating electromagnetic field--bioregenerative life support systems--degree of oxidations--denitrifying microorganisms--growth and development--higher plants--human waste--life support systems--mass exchange--microbiotas--microflora--microscopic fungi--mineralized human wastes--nutrient solution--oxidation level--phytopathogenic bacteria--plant productivity--soil-like substrate--wheat plants--biomolecules--electromagnetic fields--irrigation--magnetic field effects--metabolism--metabolites--oxidation--plants (botany)--soils--solvent extraction--wastes--waste incineration
Аннотация: To close mass exchange loops in bioregenerative life support systems more efficiently, researchers of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) have developed a procedure of wet combustion of human wastes and inedible parts of plants using H2O2 in alternating electromagnetic field. Human wastes pretreated in this way can be used as nutrient solutions to grow plants in the phototrophic unit of the LSS. The purpose of this study was to explore the possibilities of using human wastes oxidized to different degrees to grow plants cultivated on the soil-like substrate (SLS). The treated human wastes were analyzed to test their sterility. Then we investigated the effects produced by human wastes oxidized to different degrees on growth and development of wheat plants and on the composition of microflora in the SLS. The irrigation solution contained water, substances extracted from the substrate, and certain amounts of the mineralized human wastes. The experiments showed that the human wastes oxidized using reduced amounts of 30% H2O2: 1 ml/g of feces and 0.25 ml/ml of urine were still sterile. The experiments with wheat plants grown on the SLS and irrigated by the solution containing treated human wastes in the amount simulating 1/6 of the daily diet of a human showed that the degree of oxidation of human wastes did not significantly affect plant productivity. On the other hand, the composition of the microbiota of irrigation solutions was affected by the oxidation level of the added metabolites. In the solutions supplemented with partially oxidized metabolites yeast-like microscopic fungi were 20 times more abundant than in the solutions containing fully oxidized metabolites. Moreover, in the solutions containing incompletely oxidized human wastes the amounts of phytopathogenic bacteria and denitrifying microorganisms were larger. Thus, insufficiently oxidized sterile human wastes added to the irrigation solutions significantly affect the composition of the microbiological component of these solutions, which can ultimately unbalance the system as a whole. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Biel K.Y., Fomina I.R., Nazarova G.N., Soukhovolsky V.G., Khlebopros R.G., Nishio J.N.
Заглавие : Untangling metabolic and spatial interactions of stress tolerance in plants. 1. Patterns of carbon metabolism within leaves
Место публикации : Protoplasma. - 2010. - Vol. 245, Is. 1. - С. 49-73. - ISSN 0033183X (ISSN) , DOI 10.1007/s00709-010-0135-7
Ключевые слова (''Своб.индексиров.''): carbon metabolism--leaf anatomy--leaf form and function--maximal ecological utility--photosynthesis--stress tolerance spinacia oleracea--aspartate aminotransferase isoenzyme 1--bicarbonate--carbon--carbon dioxide--catalase--chlorophyll--malate dehydrogenase--oxygen--ribulosebisphosphate carboxylase--vegetable protein--article--enzymology--histology--light--metabolism--oxidation reduction reaction--photosynthesis--physiological stress--physiology--plant leaf--spinach--theoretical model--aspartate aminotransferase, cytoplasmic--bicarbonates--carbon--carbon dioxide--catalase--chlorophyll--light--malate dehydrogenase--models, theoretical--oxidation-reduction--oxygen--photosynthesis--plant leaves--plant proteins--ribulose-bisphosphate carboxylase--spinacia oleracea--stress, physiological--spinacia oleracea
Аннотация: The localization of the key photoreductive and oxidative processes and some stress-protective reactions within leaves of mesophytic C3 plants were investigated. The role of light in determining the profile of Rubisco, glutamate oxaloacetate transaminase, catalase, fumarase, and cytochrome-c-oxidase across spinach leaves was examined by exposing leaves to illumination on either the adaxial or abaxial leaf surfaces. Oxygen evolution in fresh paradermal leaf sections and CO2 gas exchange in whole leaves under adaxial or abaxial illumination was also examined. The results showed that the palisade mesophyll is responsible for the midday depression of photosynthesis in spinach leaves. The photosynthetic apparatus was more sensitive to the light environment than the respiratory apparatus. Additionally, examination of the paradermal leaf sections by optical microscopy allowed us to describe two new types of parenchyma in spinach-pirum mesophyll and pillow spongy mesophyll. A hypothesis that oxaloacetate may protect the upper leaf tissue from the destructive influence of active oxygen is presented. The application of mathematical modeling shows that the pattern of enzymatic distribution across leaves abides by the principle of maximal ecological utility. Light regulation of carbon metabolism across leaves is discussed. В© 2010 Springer-Verlag.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S.E.
Заглавие : Transfer of xenobiotics through cell membranes of luminous bacteria
Место публикации : Luminescence. - 1999. - Vol. 14, Is. 5. - С. 267-270. - ISSN 15227235 (ISSN)
Ключевые слова (''Своб.индексиров.''): luminous bacteria--toxicant--ultrastructure--bacterial dna--edetic acid--toluene--xenobiotic agent--article--cell membrane--dna damage--drug effect--luminescence--metabolism--photobacterium--sensitivity and specificity--transport at the cellular level--ultrastructure--vibrio--biological transport--cell membrane--dna damage--dna, bacterial--edetic acid--luminescence--photobacterium--sensitivity and specificity--toluene--vibrio--xenobiotics
Аннотация: The influence of some chemical substances on luminous bacteria was studied to elucidate the interrelation between the xenobiotics action on bacterial luminescence and cell ultrastructure. Such substances as quinones, phenols, chlorides of heavy metals (in concentrations of substances inhibiting luminescence by 50%) resulted in damaging effects upon bacteria: a lot of cells had damage of membranes due to changes in their permeability. It was found that the high concentration of EDTA and toluene decreased the luminescence and caused the condensation of DNA-fibrils and the cell damage after long-term and short-term action. The low concentration of EDTA and toluene did not decrease the bacterial luminescence; the noticeable damage of cell membranes did not take place during short-term treatment. However, the long action of these substances changed the membrane permeability resulting in increased sensitivity of bacterial luminescence to some toxic substances. Copyright В© 1999 John Wiley & Sons, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Krylov A.V., Sushchik N.N., Malin M.I., Makhutova O.N., Chalova I.V., Kalacheva G.S.
Заглавие : Transfer of essential polyunsaturated fatty acids from an aquatic to terrestrial ecosystem through the fish-bird trophic pair
Место публикации : Doklady Biological Sciences. - 2010. - Vol. 431, Is. 1. - С. 121-123. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496610020146
Ключевые слова (''Своб.индексиров.''): fatty acid--fresh water--triacylglycerol--unsaturated fatty acid--animal--article--biological model--biomass--bird--chemistry--ecosystem--fish--food chain--metabolism--muscle--pathology--physiology--species difference--animals--biomass--birds--ecosystem--fatty acids--fatty acids, unsaturated--fishes--food chain--fresh water--models, biological--muscles--species specificity--triglycerides--aves
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A.
Заглавие : Tolerance of LSS plant component to elevated temperatures
Место публикации : Acta Astronautica. - 2002. - Vol. 50, Is. 12. - С. 759-764. - ISSN 00945765 (ISSN) , DOI 10.1016/S0094-5765(02)00010-3
Ключевые слова (''Своб.индексиров.''): metabolism--photosynthesis--rate constants--thermal effects--thermoanalysis--thermal tolerance--life support systems (spacecraft)--carbon dioxide--adaptation--article--brassicaceae--comparative study--heat--instrumentation--light--metabolism--microclimate--photosynthesis--physiology--plant physiology--wheat--adaptation, physiological--brassicaceae--carbon dioxide--ecological systems, closed--heat--life support systems--light--photosynthesis--plant physiology--triticum
Аннотация: Stability of LSS based on biological regeneration of water, air and food subject to damaging factors is largely dependent on the behavior of the photosynthesizing component represented, mainly, by higher plants. The purpose of this study is to evaluate the tolerance of uneven-aged wheat and radish cenoses to temperature effects different in time and value. Estimation of thermal tolerance of plants demonstrated that exposure for 20 h to the temperature increasing to 45В°C brought about irreversible damage both in photosynthetic processes (up to 80% of initial value) and the processes of growth and development. Kinetics of visible photosynthesis during exposure to elevated temperatures can be used to evaluate critical exposure time within the range of which the damage of metabolic processes is reversible. With varying light intensity and air temperature it is possible to find a time period admissible for the plants to stay under adverse conditions without considerable damage of metabolic processes. В© 2002 Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Efremova S.N., Puzyr S.P., Mogil'naya O.A.
Заглавие : Tissue response to biodegradable suture threads made of polyoxyalkanoates
Место публикации : Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 23-29. - ISSN 00258075 (ISSN)
Ключевые слова (''Своб.индексиров.''): biocompatibility--biodegradation--implants (surgical)--materials testing--organic polymers--polyoxyalkanoates--suture threads--biomaterials--biomaterial--polymer--animal--article--bioremediation--female--metabolism--rat--suture--wistar rat--animals--biocompatible materials--biodegradation, environmental--female--polymers--rats--rats, wistar--sutures
Аннотация: The paper presents data on the materials and methods of obtaining suture threads based on of the polymer batty acid derivatives (polyoxyalkanoates). Results of the study of tissue response to biodegradable polyoxyalkanoate suture threads in the postoperative period are analyzed. These suture threads have been shown to have the required strength and meet a number of other requirements for currently available materials.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid'ko A.F., Pugacheva I.Yu., Shevyrnogov A.P.
Заглавие : Time course of the spectral brightness of agricultural crops during the vegetation period in Krasnoyarsk krai
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - С. 114-117. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608020130
Ключевые слова (''Своб.индексиров.''): article--crop--histology--light--metabolism--plant--russian federation--season--spectroscopy--crops, agricultural--light--plants--russia--seasons--spectrum analysis
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kirensky L.V., Gitelson I.I., Terskov I.A., Kovrov B.G., Lisovsky G.M., Okladnikov Y.N.
Заглавие : Theoretical and experimental decisions in the creation of an artificial ecosystem for human life support in space.
Место публикации : Life sciences and space research. - 1971. - Vol. 9. - С. 75-80. - ISSN 00759422 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bacterium--biotechnology--chlorella--energy metabolism--human--instrumentation--man machine interaction--metabolism--microclimate--space flight--weightlessness--bacteria--biotechnology--chlorella--ecological systems, closed--energy metabolism--environment, controlled--humans--life support systems--man-machine systems--space flight--weightlessness
Аннотация: All of man's former space flights were not real ventures into space in the biological sense, as his life was supported with unregenerated earth supplies. The coming stage of space exploration requires man's long existence in the cosmos and on the other planets. This stage of man's activity outside the earth become possible only by creating small man-made ecosystems, permitting the support of his metabolism by the recycling of substances of the terrestrial biosphere. Creation of such systems is a new scientific and technical task. Man-made ecosystems are a new product of man's activity, which have no complete analogy, either in nature, or in technology. Stochastic mechanisms, which stabilize biogeocenosis, cannot be effective in small ecosystems. A technique of parametric control over biosynthesis made it possible to calculate, and put to practice, an ecosystem for man with a cyclic regeneration of the atmosphere, water and, partially, food. The specific bio-technological properties of small man-made ecosystems are being analysed. The possibility of their application for man's excursions into space and for the settlement of other planets is being considered.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S.
Заглавие : The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): ?-ketothiolase--controlled synthesis--poly(hydroxybutyrate-co-hydroxyvalerate)--ralstonia eutropha--bacteria (microorganisms)--cupriavidus necator--acetoacetyl coenzyme a--acetyl coenzyme a acyltransferase--acyl coenzyme a--acyltransferase--butyric acid derivative--carbon dioxide--fructose--hydrogen--poly(3 hydroxybutyrate) co (3 hydroxyvalerate)--poly(3-hydroxyalkanoic acid) synthase--poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)--polyester--polyhydroxyalkanoate synthase--valeric acid derivative--article--chemistry--crystallization--culture medium--metabolism--wautersia eutropha--acetyl-coa c-acyltransferase--acyl coenzyme a--acyltransferases--butyrates--carbon dioxide--crystallization--culture media--cupriavidus necator--fructose--hydrogen--polyesters--valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinova O.N., Kalacheva G.S., Grodnitskaya I.D.
Заглавие : The prospects of the use of resorbable polyesters for designing safe pesticides
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - С. 100-103. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608020099
Ключевые слова (''Своб.индексиров.''): pesticide--polyester--article--chemistry--drug design--metabolism--microbiology--drug design--pesticides--polyesters--soil microbiology
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhutova O.N., Sushchik N.N., Kalacheva G.S.
Заглавие : The information value of fatty acid composition of triacylglycerols and polar lipids of seston in assaying the food spectrum of microzooplankton in the bugach reservoir.
Место публикации : Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2004. - Vol. 395. - С. 143-145. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--fresh water--lipid--triacylglycerol--article--bacterium--biomass--chemistry--cyanobacterium--diatom--ecosystem--metabolism--physiology--phytoplankton--russian federation--season--bacteria--biomass--cyanobacteria--diatoms--ecosystem--fatty acids--fresh water--lipids--phytoplankton--russia--seasons--triglycerides
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Zhila N.O., Volova T.G., Gladyshev M.I.
Заглавие : The effect of temperature on the lipid composition of the green alga Botryococcus
Колич.характеристики :8 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2002. - Vol. 71, Is. 3. - P286-293. - ISSN 0026-2617, DOI 10.1023/A:1015898426573
Примечания : Cited References: 14
Предметные рубрики: METABOLISM
CULTURES
BRAUNII
Ключевые слова (''Своб.индексиров.''): green alga--lipids--fatty acids--temperature
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal (18degreesC), optimal (25degreesC), and supraoptimal (32degreesC). Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Zhila N.O., Volova T.G., Gladyshev M.I.
Заглавие : The effect of temperature on the lipid composition of Botryococcus
Место публикации : Mikrobiologiya. - 2002. - Vol. 71, Is. 3. - С. 336-344. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--lipid--triacylglycerol--article--biosynthesis--chemistry--comparative study--green alga--growth, development and aging--metabolism--temperature--algae, green--fatty acids--lipids--temperature--triglycerides
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal, optimal, and supraoptimal. Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Barashkov V.A., Gitelzon I.I., Nefedov V.P., Trubachev I.N.
Заглавие : The dynamics of the amino acid composition of the medium in cultivating the isolated organs by controlled perfusion (Russian)
Место публикации : Byulleten Eksperimentalnoi Biologii i Meditsiny. - 1975. - Vol. 80, Is. 7. - С. 36-38. - ISSN 03659615 (ISSN)
Ключевые слова (''Своб.индексиров.''): amino acid--amino acid--dog--in vitro study--kidney--liver--sternum--theoretical study--animal--article--culture medium--metabolism--organ culture--organ preservation--perfusion--tissue preservation--kidney--liver--kidney perfusion--liver perfusion--tissue culture--amino acids--animal--culture media--dogs--english abstract--in vitro--organ culture--organ preservation--perfusion--tissue preservation--kidney--liver
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--alkane--carbon monoxide--fatty acid--hydroxyacid--lipid--nitrogen--polyester--article--chemistry--culture medium--drug antagonism--growth, development and aging--mass spectrometry--metabolism--wautersia eutropha--alkanes--carbon monoxide--culture media--cupriavidus necator--fatty acids--hydroxy acids--lipids--mass spectrometry--nitrogen--polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Yu.A., Koyalev V.S., Gribovskaya I.V., Tirranen L.S., Zolotukhin I.G., Gros J.B., Lasseur Ch.
Заглавие : Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system
Место публикации : Acta Astronautica. - 2003. - Vol. 53, Is. 4-10. - С. 249-257. - ISSN 00945765 (ISSN) , DOI 10.1016/S0094-5765(03)00137-1
Ключевые слова (''Своб.индексиров.''): ecosystems--microorganisms--ph--photosynthesis--plants (botany)--synthesis (chemical)--waste utilization--biological life support systems (blls)--gas exchange--plant respiration--biomass--carbon dioxide--agaricales--article--biomass--bioremediation--growth, development and aging--hydroponics--incineration--metabolism--methodology--microbiology--microclimate--photosynthesis--plant physiology--radish--space flight--waste management--weightlessness--wheat--agaricales--biodegradation, environmental--biomass--carbon dioxide--ecological systems, closed--environmental microbiology--hydroponics--incineration--life support systems--photosynthesis--plant physiology--raphanus--space flight--triticum--waste management--weightlessness
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Califomian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. В© 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S., Bondar' V.S.
Заглавие : Study of the luminescence system of the soil enchytraeid Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady Biochemistry and Biophysics. - 2003. - Vol. 391, Is. 1-6. - С. 204-207. - ISSN 16076729 (ISSN) , DOI 10.1023/A:1025105323648
Ключевые слова (''Своб.индексиров.''): adsorption chromatography--animal cell--annelid worm--article--luminescence--nonhuman--purification--animal--drug antagonism--isolation and purification--metabolism--physiology--soil--animalia--annelida--clitellata--enchytraeidae--edetic acid--luciferase--magnesium--animals--edetic acid--luciferases--luminescent measurements--magnesium--oligochaeta--soil
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