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Reusable biochemical diagnosis systems based on nanodiamonds / V. S. Bondar [et al.] // Doklady Biochemistry and Biophysics. - 2013. - Vol. 448, Is. 1. - P55-58, DOI 10.1134/S160767291301016X . - ISSN 1607-6729
Кл.слова (ненормированные):
cholesterol -- diamond -- nanoparticle -- article -- bioassay -- blood -- chemical model -- chemistry -- glucose blood level -- human -- methodology -- Biological Assay -- Blood Glucose -- Cholesterol -- Diamond -- Humans -- Models, Chemical -- Nanoparticles

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondar, V.S.; Ronzhin, N.O.; Mamaeva, E.S.; Baron, A.V.; Gitelson, J.I.

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Assessment of composition and toxicity for plants of gases produced during physicochemical processing of human exometabolites as applied to biotechnical life support systems / A. A. Tikhomirov [et al.] // Doklady Biochemistry and Biophysics. - 2011. - Vol. 441, Is. 1. - P252-254, DOI 10.1134/S1607672911060032 . - ISSN 1607-6729
Кл.слова (ненормированные):
ammonia -- carbon dioxide -- nitrogen oxide -- oxygen -- article -- biomass -- bioremediation -- drug effect -- gas -- growth, development and aging -- human -- instrumentation -- methodology -- microclimate -- plant -- waste management -- Ammonia -- Biodegradation, Environmental -- Biomass -- Carbon Dioxide -- Ecological Systems, Closed -- Gases -- Humans -- Life Support Systems -- Nitrogen Oxides -- Oxygen -- Plants -- Waste Management

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Boreskov Institute of Catalysis, Omsk Branch, Siberian Branch, Russian Academy of Sciences, ul. Neftezavodskaya 54, Omsk 644053, Russian Federation
Siberian Federal University, Svobodnyi pr. 41, Krasnoyarsk 660079, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Kudenko, Y.A.; Degermendzhi, A.G.; Trifonov, S.V.; Sutormina, E.F.; Ivanova, Y.A.

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Protein-protein complexation in bioluminescence [Text] / M. S. Titushin [et al.] // Protein Cell. - 2011. - Vol. 2, Is. 12. - P957-972, DOI 10.1007/s13238-011-1118-y. - Cited References: 114. - The work was funded by "Fellowship for Young International Scientists" of Chinese Academy of Sciences. This work was supported by the National Natural Science Foundation of China (Grant Nos: 30870483, 31070660, 31021062 and 81072449), Ministry of Science and Technology of China (Nos. 2009DFB30310, 2009CB918803 and 2011CB911103), CAS Research Grants (Nos. YZ200839 and KSCX2-EW-J-3). . - ISSN 1674-800X

РУБ Cell Biology
Рубрики:
GREEN-FLUORESCENT PROTEIN
   LUCIFERIN-BINDING-PROTEIN
   RENILLA-RENIFORMIS LUCIFERASE
   VIBRIO-FISCHERI Y1
   JELLYFISH CLYTIA-GREGARIA
   ALPHA/BETA-HYDROLASE FOLD
   AMINO-ACID-SEQUENCE
   BACTERIAL LUCIFERASE
   ENERGY-TRANSFER
   CRYSTAL-STRUCTURE
Кл.слова (ненормированные):
green-fluorescent protein (GFP) -- photoprotein -- luciferase -- lumazine protein -- Forster resonance energy transfer (FRET) -- docking
Аннотация: In this review we summarize the progress made towards understanding the role of protein-protein interactions in the function of various bioluminescence systems of marine organisms, including bacteria, jellyfish and soft corals, with particular focus on methodology used to detect and characterize these interactions. In some bioluminescence systems, protein-protein interactions involve an "accessory protein" whereby a stored substrate is efficiently delivered to the bioluminescent enzyme luciferase. Other types of complexation mediate energy transfer to an "antenna protein" altering the color and quantum yield of a bioluminescence reaction. Spatial structures of the complexes reveal an important role of electrostatic forces in governing the corresponding weak interactions and define the nature of the interaction surfaces. The most reliable structural model is available for the protein-protein complex of the Ca2+-regulated photoprotein clytin and green-fluorescent protein (GFP) from the jellyfish Clytia gregaria, solved by means of X-ray crystallography, NMR mapping and molecular docking. This provides an example of the potential strategies in studying the transient complexes involved in bioluminescence. It is emphasized that structural studies such as these can provide valuable insight into the detailed mechanism of bioluminescence.

Держатели документа:
[Titushin, Maxim S.
Liu, Zhi-Jie] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
[Feng, Yingang] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
[Lee, John] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
[Vysotski, Eugene S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Lab Photobiol, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Titushin, M.S.; Feng, Y.G.; Lee, J...; Vysotski, E.S.; Liu, Z.J.

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Detoxification of AM-241 solutions by humic substances: Bioluminescent monitoring / T. Rozhko [et al.] // Analytical and Bioanalytical Chemistry. - 2011. - Vol. 400, Is. 2. - P329-334, DOI 10.1007/s00216-010-4442-9 . - ISSN 1618-2642
Кл.слова (ненормированные):
Detoxification -- Humic substances -- Ionizing radiation -- Luminous bacteria -- Bacterial cells -- Bottom sediments -- Humic substances -- Luminescent intensity -- Luminous bacteria -- Natural transformations -- Organic substances -- Photobacterium phosphoreum -- Physiological activity -- Protecting agent -- Water solutions -- Anoxic sediments -- Bacteriology -- Bioluminescence -- Detoxification -- Ionizing radiation -- Luminance -- Radiation shielding -- Radioactivity -- Bacteria -- americium -- radioisotope -- article -- bioremediation -- chemistry -- environmental monitoring -- evaluation -- humic substance -- instrumentation -- luminescence -- metabolism -- methodology -- Photobacterium -- water pollutant -- Americium -- Biodegradation, Environmental -- Environmental Monitoring -- Humic Substances -- Luminescence -- Photobacterium -- Radioisotopes -- Water Pollutants, Chemical -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: The study addresses the effect of humic substances on marine luminous bacteria Photobacterium phosphoreum exposed to Am-241 (3,000 Bq L -1, water solution). Luminescent intensity of the bacteria was applied as a marker of their physiological activity. Humic substances have been found to reduce the effect of Am-241 on luminescence, decrease damage to cells, and change distribution of Am-241 between bacterial cells and intercellular media. It was shown that water-soluble humic substances, being products of natural transformation of organic substances in soil and bottom sediments, can serve as protecting agents for water microorganisms exposed to alpha radionuclides. В© 2010 Springer-Verlag.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk 660036, Russian Federation
Irkutsk State University, Irkutsk 664003, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rozhko, T.; Bondareva, L.; Mogilnaya, O.; Vydryakova, G.; Bolsunovsky, A.; Stom, D.; Kudryasheva, N.

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Mathematical model of seasonal agrophytocenosis productivity based on terrestrial and satellite monitoring / T. I. Pisman [et al.] // Doklady Biological Sciences. - 2009. - Vol. 428, Is. 1. - P467-470, DOI 10.1134/S0012496609050226 . - ISSN 0012-4966
Кл.слова (ненормированные):
agriculture -- algorithm -- article -- biological model -- biomass -- computer simulation -- crop -- growth, development and aging -- methodology -- season -- space flight -- wheat -- Agriculture -- Algorithms -- Biomass -- Computer Simulation -- Crops, Agricultural -- Models, Biological -- Seasons -- Spacecraft -- Triticum

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50.50, Krasnoyarsk 660036, Russian Federation
Institute of Natural Sciences and Mathematics, Khakass State University, pr. Lenina 90, Abakan, 655000 Khakassia, Russian Federation
Institute of Space and Information Technologies, Siberian Federal University, ul. Kirenskogo 26, Krasnoyarsk 660074, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Pugacheva, I.Y.; Jukova, E.Y.; Shevyrnogov, A.P.

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Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of Lake Shira as an indicator of human impact on the ecosystem / T. I. Lobova [et al.] // Microbiological Research. - 2008. - Vol. 163, Is. 2. - P152-160, DOI 10.1016/j.micres.2006.03.014 . - ISSN 0944-5013
Кл.слова (ненормированные):
Antibiotic resistance -- Aquatic ecosystems -- Heterotrophic bacteria -- Human impact -- Monitoring -- Antibiotics -- Bacteria -- Ecosystems -- Environmental impact -- Mammals -- Antibiotic resistance -- Aquatic ecosystems -- Heterotrophic bacteria -- Materials -- antiinfective agent -- fresh water -- allochthony -- anthropogenic effect -- antibiotic resistance -- bacterium -- concentration (composition) -- heterotrophy -- intertidal environment -- monitoring -- recreational facility -- spring (season) -- summer -- animal -- article -- bacterial count -- bacterium -- drug effect -- ecosystem -- environmental monitoring -- heterotrophy -- human -- isolation and purification -- methodology -- microbiological examination -- microbiology -- multidrug resistance -- Russian Federation -- season -- Animals -- Anti-Bacterial Agents -- Bacteria -- Colony Count, Microbial -- Drug Resistance, Multiple, Bacterial -- Ecosystem -- Environmental Monitoring -- Fresh Water -- Heterotrophic Processes -- Humans -- Microbial Sensitivity Tests -- Russia -- Seasons -- Eurasia -- Khakassia -- Lake Shira -- Russian Federation -- Animalia -- Bacteria (microorganisms)
Аннотация: Resistance to Ampicillin and Kanamycin displayed by heterotrophic bacteria isolated in Summer and in Spring from the littoral and the central parts of Lake Shira (a therapeutic lake in the Khakasia Republic, Russia) has been investigated. It has been found that in Summer, human and animal microflora featuring multiple antibiotic resistance (to Ampicillin and Kanamycin) predominates in all the studied stations of the littoral zone of the lake. In Spring, concentrations of bacteria featuring multiple antibiotic resistance decrease significantly and bacteria sensitive to antibiotics predominate in the lake. Emergence of multiple antibiotic resistance in bacteria of Lake Shira is caused by the input of allochthonous bacteria into the lake; this feature of heterotrophic bacteria of Lake Shira can be used to monitor the impact on the ecosystem made by health resorts. В© 2006 Elsevier GmbH. All rights reserved.

Scopus
Держатели документа:
LTD Territory-oriented information systems, Institute of Computational Modeling, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok 50, 660036, Russian Federation
Center of Hygiene and Epidemiology in Krasnoyarsk Region, Sopochnaya 38, Russian Federation
Krasnoyarsk Scientific Centre, Akademgorodok 50, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Barkhatov, Y.V.; Salamatina, O.V.; Popova, L.Yu.

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Some methods for human liquid and solid waste utilization in bioregenerative life-support systems / S. A. Ushakova [et al.] // Applied Biochemistry and Biotechnology. - 2008. - Vol. 151, Is. 2-3. - P676-685, DOI 10.1007/s12010-008-8291-3 . - ISSN 0273-2289
Кл.слова (ненормированные):
Closure -- Human waste -- Life-support systems -- Salicornia -- Sodium chloride -- Above-ground biomass -- Biological lives -- Bioregenerative -- Closure -- Cultivation process -- Culture methods -- Human waste -- Irrigation waters -- Life-support systems -- Manned space missions -- Mineral elements -- Physico-chemical methods -- Salicornia -- Salicornia europaea -- Salt-tolerant -- Soil-like substrates -- Biomass -- Body fluids -- Electrodialysis -- Grain (agricultural product) -- Irrigation -- Liquids -- Metal refining -- Minerals -- Mining -- Oxidation -- Plant shutdowns -- Sodium chloride -- Soils -- Solid wastes -- Substrates -- Water supply -- Vegetation -- article -- biomass -- controlled study -- electrodialysis -- halophyte -- irrigation (agriculture) -- microclimate -- nonhuman -- recycling -- Salicornia europaea -- solid waste -- bioremediation -- dialysis -- feces -- goosefoot -- growth, development and aging -- human -- methodology -- salt tolerance -- urine -- waste management -- wheat -- Batis maritima -- Salicornia -- Salicornia europaea -- Triticum aestivum -- Biodegradation, Environmental -- Chenopodiaceae -- Dialysis -- Feces -- Humans -- Life Support Systems -- Salt-Tolerance -- Triticum -- Urine -- Waste Management
Аннотация: Bioregenerative life-support systems (BLSS) are studied for developing the technology for a future biological life-support system for long-term manned space missions. Ways to utilize human liquid and solid wastes to increase the closure degree of BLSS were investigated. First, urine and faeces underwent oxidation by Kudenko's physicochemical method. The products were then used for root nutrition of wheat grown by the soil-like substrate culture method. Two means of eliminating sodium chloride, introduced into the irrigation solution together with the products of urine oxidation, were investigated. The first was based on routine electrodialysis of irrigation water at the end of wheat vegetation. Dialysis eliminated about 50% of Na from the solution. This desalinization was performed for nine vegetations. The second method was new: after wheat cultivation, the irrigation solution and the solution obtained by washing the substrate containing mineral elements not absorbed by the plants were used to grow salt-tolerant Salicornia europaea L. plants (saltwort). The above-ground biomass of this plant can be used as a food, and roots can be added to the soil-like substrate. Four consecutive wheat and Salicornia vegetations were cultivated. As a result of this wheat and Salicornia cultivation process, the soil-like substrate salinization by NaCl were considerably decreased. В© 2008 Humana Press.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, 660036 Krasnoyarsk, Russian Federation
K.A. Timiraziev Institute of Plant Physiology, Russian Academy of Science, 35 Botanisheskaya, 127276 Moscow, Russian Federation
LGCB, Universite Blaise Pascal, Polytech'Clermont-Ferrand, P.O. Box 206, 63174 Aubiere cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Zolotukhin, I.G.; Tikhomirov, A.A.; Tikhomirova, N.A.; Kudenko, Yu.A.; Gribovskaya, I.V.; Balnokin, Yu.; Gros, J.B.

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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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Management and control of microbial populations' development in LSS of missions of different durations / L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1621-1625, DOI 10.1016/j.asr.2004.12.046 . - ISSN 0273-1177
Кл.слова (ненормированные):
Long-term missions -- LSS -- Macro- and microorganism -- Microbial populations -- Ecosystems -- Population statistics -- Probability -- Recycling -- Life support systems (LSS) -- Long-term mission -- Macro-and microorganism -- Microbial populations -- Microorganisms -- aerospace medicine -- bioreactor -- bioremediation -- Chlorella -- conference paper -- drug resistance -- human -- hygiene -- immune system -- instrumentation -- methodology -- microbiology -- microclimate -- physiology -- space flight -- waste management -- weightlessness -- Aerospace Medicine -- Biodegradation, Environmental -- Bioreactors -- Chlorella -- Drug Resistance -- Ecological Systems, Closed -- Environmental Microbiology -- Humans -- Hygiene -- Immune System -- Life Support Systems -- Space Flight -- Waste Management -- Weightlessness
Аннотация: The problem of interaction between man and microorganisms in closed habitats is an inextricable part of the whole problem of co-existence between macro- and microorganisms. Concerning the support of human life in closed habitat, we can, conventionally, divide microorganisms, acting in life support system (LSS) into three groups: useful, neutral and harmful. The tasks, for human beings for optimal coexistence with microhabitants seem to be trivial: (1) to increase the activity of useful forms, (2) decrease the activity harmful forms, (3) not allow the neutral forms to become the harmful ones and even to help them to gain useful activity. The task of efficient management and control of microbial population's development in LSS highly depends on mission duration. As for short-term missions without recycling, the proper hygienic procedures are developed. For longer missions, the probability of transformation of the neutral forms into the harmful ones is becoming more dangerous. The LSS for long-term missions are to use cycling-recycling systems, including system with biological recycling. In these systems, microbial populations as regenerative link should be useful and active agents. Some problems of microbial populations control and management are discussed in the paper. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.

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10.

Trends in microevolution of microbial populations in open systems / A. V. Brilkov [et al.] // Doklady Biochemistry and Biophysics. - 2005. - Vol. 404, Is. 1-6. - P349-352, DOI 10.1007/s10628-005-0111-x . - ISSN 1607-6729
Кл.слова (ненормированные):
article -- bacterial phenomena and functions -- bacterium -- biological model -- culture technique -- Escherichia coli -- evolution -- genetics -- growth, development and aging -- mathematics -- methodology -- mutation -- nanotechnology -- pH -- physiology -- population dynamics -- time -- Bacteria -- Bacterial Physiology -- Cell Culture Techniques -- Escherichia coli -- Evolution -- Hydrogen-Ion Concentration -- Mathematics -- Models, Biological -- Mutation -- Nanotechnology -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Brilkov, A.V.; Loginov, I.A.; Morozova, E.V.; Pechurkin, N.S.

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11.

Developing the control criterion for a continuous culture of microorganisms / V. V. Adamovich, D. Yu. Rogozin, A. G. Degermendzhi // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 5-16 . - ISSN 0026-3656
Кл.слова (ненормированные):
Chemostat -- Control criterion -- Control factor -- Microorganism population -- Sensitivity coefficients -- algorithm -- bacterium -- biological model -- biomass -- culture medium -- ecosystem -- growth, development and aging -- methodology -- microbiological examination -- review -- Algorithms -- Bacteria -- Bacteriological Techniques -- Biomass -- Culture Media -- Ecosystem -- Models, Biological
Аннотация: A short survey and critical analysis of previously proposed criteria for growth control of populations of microorganisms in the chemostat are presented. Based on the analysis of a mathematical model of the steady-state of a microbial population in the chemostat, an adequate control criterion is suggested, along with a method to identify the corresponding regulating factors. The new control criterion is expressed as a product of the factor transformation coefficient and the biomass sensitivity coefficient (SC) with respect to the change of the factor at the chemostat inlet (referred to in the sequel as the biomass SC). The control criterion determines the strength of the control exerted by this or that factor. The method of determination of the regulating factors consists in experimental determination of the real SCs for factors and the biomass and in calculating on this basis the corresponding ideal SCs for constant factor transformation coefficients. The ideal SCs are shown to add up to an integer value, a constraint that we call "quantization" relationships. Such relationships are used to test the completeness of the drawn list of control factors. The proposed method was applied to our own and literature data.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Adamovich, V.V.; Rogozin, D.Yu.; Degermendzhi, A.G.

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12.

Principles of biological adaptation of organisms in artificial ecosystems to changes of environmental factors / L. A. Somova, N. S. Pechurkin, T. I. Pisman // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1512-1515, DOI 10.1016/j.asr.2004.12.038 . - ISSN 0273-1177
Кл.слова (ненормированные):
Artificial ecosystems -- Biotic cycles -- Microorganisms -- Energy utilization -- Environmental impact -- Microorganisms -- Plants (botany) -- Purification -- Water -- AES -- Artificial ecosystems -- Biotic cycles -- Material transformations -- Ecosystems -- bioremediation -- comparative study -- conference paper -- ecosystem -- methodology -- microbiology -- microclimate -- plant -- sewage -- waste management -- water management -- Biodegradation, Environmental -- Ecological Systems, Closed -- Ecosystem -- Environmental Microbiology -- Life Support Systems -- Plants -- Sewage -- Soil Microbiology -- Waste Management -- Water Purification
Аннотация: Studying material transformations and biotic cycling in artificial ecosystems (AES), we need to know the principles of biological adaptation of active organisms to change in the environment. Microorganisms in AES for water purification are the most active transforming organisms and consumers of the organic substances contained in wastes. Utilization of organic substances is directly connected with the energy fluxes used by AES. According to energy criteria, the energy fluxes used by a biological system tend to reach maximum values under stable conditions. Unutilized substrate concentration decreases as a result of biological adaptations. After a dramatic change in environmental factors, for example, after a sharp increase in the flow rate of organic substances, the biological system is not able to react quickly. The concentration of unutilized substrate increases and the energy flux used by the biological system decreases. The structure of the microbial community also changes, with a decrease in biological diversity. The efficiency of energy use by simple terrestrial ecosystems depends on the energetic intensity and interactions between plants and rhizospheric microorganisms. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk 36, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Pisman, T.I.

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13.

Accumulation and release of 241Am by a macrophyte of the Yenisei River (Elodea canadensis) / A. Bolsunovsky, T. Zotina, L. Bondareva // Journal of Environmental Radioactivity. - 2005. - Vol. 81, Is. 1. - P33-46, DOI 10.1016/j.jenvrad.2004.10.012 . - ISSN 0265-931X
Кл.слова (ненормированные):
241Am -- Accumulation -- Laboratory experiments -- Release -- Submerged plant Elodea canadensis -- Yenisei River -- Activation analysis -- Biomass -- Concentration (process) -- Effluents -- Plutonium -- Rivers -- Aquatic plants -- Elodea plant -- Macrophytes -- Radioactive contamination -- Transuranium elements -- americium 241 -- river water -- americium -- americium nitrate -- bioaccumulation -- biological uptake -- macrophyte -- pollutant source -- radioactive pollution -- river water -- submerged vegetation -- article -- biomass -- concentration (parameters) -- environmental factor -- fractionation -- laboratory -- macrophyte -- radioactivity -- river -- sampling -- adsorption -- chemistry -- Hydrocharitaceae -- methodology -- physiology -- plant -- radiation exposure -- radiation monitoring -- Russian Federation -- sediment -- soil pollutant -- time -- tissue distribution -- water pollutant -- Eastern Hemisphere -- Eurasia -- Russian Federation -- World -- Yenisei River -- Elodea canadensis -- Adsorption -- Americium -- Biomass -- Chemical Fractionation -- Geologic Sediments -- Hydrocharitaceae -- Plant Shoots -- Radiation Monitoring -- Rivers -- Russia -- Soil Pollutants, Radioactive -- Time Factors -- Tissue Distribution -- Water Pollutants, Radioactive
Аннотация: The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of 241Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that 241Am is accumulated by Elodea biomass: the activity concentration of 241Am can reach 3280 В± 240 Bq/g, with the concentration factor for 241Am 16 600 В± 2200 l/kg. Results of chemical fractionation have proved that in the course of 241Am accumulation by Elodea biomass, 241Am tightly bound to biomass increases from 11% to 27% of the total 241Am in the biomass. Release of 241Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial 241Am activity and the rate of 241Am release into the water environment reached 23 Bq/day. В© 2004 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Zotina, T.; Bondareva, L.

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14.

Rubomycin microincapsulation with biodegradable polymer matrix / E. I. Shishatskaya // Antibiotiki i Khimioterapiya. - 2005. - Vol. 50, Is. 8-9. - С. 4-9 . - ISSN 0235-2990
Кл.слова (ненормированные):
Daunorubicin (rubomycin) -- Microspheres -- Polyhydroxybutirate -- antineoplastic antibiotic -- daunorubicin -- hydroxybutyric acid -- microsphere -- poly(3 hydroxybutyric acid) -- poly-beta-hydroxybutyrate -- polyester -- polymer -- article -- chemistry -- delayed release formulation -- electronics -- methodology -- microcapsule -- temperature -- Antibiotics, Antineoplastic -- Capsules -- Daunorubicin -- Delayed-Action Preparations -- Hydroxybutyrates -- Microspheres -- Miniaturization -- Polyesters -- Polymers -- Temperature
Аннотация: A procedure for preparation of microspheres from biodegradable linear polyether of microbiological origin (polyhydroxybutirate, PHB) with using the technology of solvent evaporation was developed considering a specific example of two- and three-component emulsions. The procedure provided permanent preparation of the microspheres of high quality. The influence of the procedure (emulsion type, dispersion process and medium temperature) on the yield of the microspheres, their structure and size was shown. The temperature had a significant impact on incorporation of the antitumor anthracycline antibiotic daunorubicin (rubomycin) to the polymer matrix. The microspheres with various levels of the drug load (29 and 90% of the initial content in the emulsion) were prepared and the kinetics of the in vitro rubomycin release was studied. The dynamics of the highly toxic rubomycin release from the microspheres was on the whole even with the curve profile reaching the plateau in 20-22 hours of the observation period. The rate of the rubomycin release to the medium depended on the value of the antibiotic incorporation and was maximum within the first two hours (3.3 and 13.0 mcg/mlВ·h) that corresponded to the release of 0.97 and 3.89 of the incorporated antibiotic. The average rate of rubomycin release during 300 hours was 0.81В·10-4 and 2.3В·10-4 mcg/mlВ·h. The release constituted respectively 3.9 and 13.11% of the antibiotic incorporated to the microspheres.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.

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15.

Biological and physicochemical methods for utilization of plant wastes and human exometabolites for increasing internal cycling and closure of life support systems / I. G. Zolotukhin [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1559-1562, DOI 10.1016/j.asr.2005.01.006 . - ISSN 0273-1177
Кл.слова (ненормированные):
BLSS -- Desalting -- Higher plants -- NaCl utilization -- SLS -- Biomass -- Crops -- Decomposition -- Electrodialysis -- Harvesting -- Metabolites -- Soils -- Wastes -- BLSS -- Higher plants -- NaCl utilization -- SLS -- Plants (botany) -- Biomass -- Decay -- Deionization -- Harvesting -- Plants -- Soil -- Wastes -- Wheat -- sodium chloride -- article -- biomass -- bioremediation -- culture medium -- feces -- growth, development and aging -- human -- metabolism -- methodology -- microbiology -- microclimate -- urine -- waste management -- wheat -- Biodegradation, Environmental -- Biomass -- Culture Media -- Ecological Systems, Closed -- Feces -- Humans -- Life Support Systems -- Sodium Chloride -- Soil Microbiology -- Triticum -- Urine -- Waste Management
Аннотация: Wheat was cultivated on soil-like substrate (SLS) produced by the action of worms and microflora from the inedible biomass of wheat. After the growth of the wheat crop, the inedible biomass was restored in SLS and exposed to decomposition ("biological" combustion) and its mineral compounds were assimilated by plants. Grain was returned to the SLS in the amount equivalent to human solid waste produced by consumption of the grain. Human wastes (urine and feces) after physicochemical processing turned into mineralized form (mineralized urine and mineralized feces) and entered the plants' nutrient solution amounts equal to average daily production. Periodically (once every 60-70 days) the nutrient solution was partly (up to 50%) desalinated by electrodialysis. Due to this NaCl concentration in the nutrient solution was sustained at a fixed level of about 0.26%. The salt concentrate obtained could be used in the human nutrition through NaCl extraction and the residuary elements were returned through the mineralized human liquid wastes into matter turnover. The control wheat cultivation was carried out on peat with use of the Knop nutrient solution. Serial cultivation of several wheat vegetations within 280 days was conducted during the experiment. Grain output varied and yield/harvest depended, in large part, upon the amount of inedible biomass returned to SLS and the speed of its decomposition. After achieving a stationary regime, (when the quantity of wheat inedible biomass utilized during vegetation in SLS is equal to the quantity of biomass introduced into SLS before vegetation) grain harvest in comparison with the control was at most 30% less, and in some cases was comparable to the control harvest values. The investigations carried out on the wheat example demonstrated in principle the possibility of long-term functioning of the LSS photosynthesizing link based on optimizations of biological and physicochemical methods of utilization of the human and plants wastes. The possibilities for the use of these technologies for the creation integrated biological-physicochemical LSS with high closure degree of internal matter turnover are discussed in this paper. В© 2005 Published by Elsevier Ltd on behalf of COSPAR.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zolotukhin, I.G.; Tikhomirov, A.A.; Kudenko, Yu.A.; Gribovskaya, I.V.

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16.

Material balance and diet in bioregenerative life support systems: Connection with coefficient of closure / N. S. Manukovsky [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1563-1569, DOI 10.1016/j.asr.2005.01.002 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bioregenerative life support system -- Coefficient of closure -- Diet -- Soil-like substrate -- Additives -- Degradation -- Materials balance -- Nitrogen -- Nutrition -- Stoichiometry -- Bioregenerative life support systems -- Coefficient of closure -- Soil-like substrates -- Life support systems (spacecraft) -- vegetable protein -- biomass -- conference paper -- culture medium -- diet -- feces -- growth, development and aging -- human -- methodology -- microclimate -- plant -- urine -- waste management -- Biomass -- Culture Media -- Diet -- Ecological Systems, Closed -- Feces -- Humans -- Life Support Systems -- Plant Proteins -- Plants, Edible -- Urine -- Waste Management
Аннотация: Bioregenerative life support systems (BLSS) with different coefficients of closure are considered. The 66.2% coefficient of closure achieved in "BIOS-3" facility experiments has been taken as a base value. The increase in coefficient of closure up to 72.6-93.0% is planned due to use of soil-like substrate (SLS) and concentrating of urine. Food values were estimated both in a base variant ("BIOS-3"), and with increases in the coefficient of closure. It is shown that food requirements will be more fully satisfied by internal crop production with an increase in the coefficient of closure of the BLSS. Changes of massflow rates on an 'input-output' and inside BLSS are considered. Equations of synthesis and degradation of organic substances in BLSS were examined using a stoichiometric model. The paper shows that at incomplete closure of BLSS containing SLS there is a problem of nitrogen balancing. To compensate for the removal of nitrogen from the system in urine and feces, it is necessary to introduce food and a nitrogen-containing additive. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Academgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Manukovsky, N.S.; Kovalev, V.S.; Somova, L.A.; Gurevich, Yu.L.; Sadovsky, M.G.

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17.

Algorithms of self-adaptation for atmospheric model designing [Text] / J. P. Lankin, T. F. Baskanova ; ed.: GG Matvienko, o, GG Matvie // TENTH JOINT INTERNATIONAL SYMPOSIUM ON ATMOSPHERIC AND OCEAN OPTICS/ATMOSPHERIC PHYSICS, PT 2: LASER SENSING AND ATMOSPHERIC PHYSICS. Ser. PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) : SPIE-INT SOC OPTICAL ENGINEERING, 2004. - Vol. 5397: 10th Joint International Symposium on Atmospheric and Ocean Optics/Atmospheric Physics (JUN 24-28, 2003, Tomsk, RUSSIA). - P. 260-270, DOI 10.1117/12.548609. - Cited References: 23 . - ISBN 0277-786X. - ISBN 0-8194-5316-1

РУБ Environmental Sciences + Meteorology & Atmospheric Sciences + Optics

Аннотация: The paper describes the principal limitations of the traditional methods used to construct atmospheric models. These limitations would not allow any fundamental improvement of atmospheric modeling. Ways are proposed to overcome the current limitations, based oil the methodology of constructing adaptive models and neuroinformatics. Algorithms of self-adaptation for neural networks intended for the construction of atmospheric models are given. Essentially, the developed algorithms are adaptive shells and can be easily transferred to other models.

WOS
Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lankin, J.P.; Baskanova, T.F.; Matvienko, GG \ed.\; Matvie, o, GG \ed.\

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18.

Radioactive contamination of aquatic organisms of the Yenisei river in the area affected by the activity of the mining-and-chemical combine / A. Ya. Bolsunovskij, A. G. Sukovatyj // Radiatsionnaya Biologiya. Radioekologiya. - 2004. - Vol. 44, Is. 3. - С. 361-366 . - ISSN 0869-8031
Кл.слова (ненормированные):
Dosimetry -- Environmental impact -- Radioactivity -- River pollution -- Water analysis -- Aquatic organisms -- Diatoms -- Exposure dose rate -- Fontinalis antipyretica -- Phylolimnogammarus viridis -- The Enisei River -- Radioisotopes -- Animalia -- Bacillariophyta -- Bryophyta -- Fontinalis -- Fontinalis antipyretica -- fresh water -- radioisotope -- animal -- article -- chemical industry -- Crustacea -- diatom -- environmental monitoring -- industrial waste -- methodology -- mining -- plant -- radiation exposure -- radiation response -- Russian Federation -- water pollutant -- water pollution -- Animals -- Chemical Industry -- Crustacea -- Diatoms -- Dose-Response Relationship, Radiation -- Environmental Monitoring -- Fresh Water -- Industrial Waste -- Mining -- Plants -- Radioisotopes -- Siberia -- Water Pollutants, Radioactive -- Water Pollution
Аннотация: The study was done to investigate the content of manmade radionuclides in aquatic organisms of the Yenisei River near the Mining-and-Chemical Combine (MCC) and to estimate the exposure dose rates to organisms from various sources. The results of the investigation and calculations suggest that the main source of radioactive contamination of aquatic organisms is the coolant of the third MCC reactor, which is still being released into the Yenisei. Gamma-spectrometric analysis revealed 23 manmade radionuclides in the biomass of aquatic plants. The aquatic animal Phylolimnogammarus viridis and diatoms also contain manmade radionuclides. Among aquatic organisms, the highest dose rate is received by aquatic plants (up to 39 ?Gy/day). For most aquatic organisms under study, the dose received from the technogenic irradiation is an order of magnitude higher than the dose received from natural irradiation. The water moss (Fontinalis antipyretica) features the highest capacity to accumulate manmade radionuclides; hence, it accumulates the largest technogenic exposure dose among the study aquatic organisms.

Scopus
Держатели документа:
Inst. of Biophysics, Sib. Div. of RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Sukovatyj, A.G.

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19.

Assessment of the rate of accumulation of the transuranium element americium-241 by the aquatic plant Elodea canadensis / A. Ya. Bolsunovskii [et al.] // Doklady Biological Sciences. - 2004. - Vol. 399, Is. 1-6. - P467-469, DOI 10.1007/s10630-005-0014-0 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- radioisotope -- water -- article -- biomass -- drug effect -- environmental monitoring -- Hydrocharitaceae -- methodology -- pH -- plant -- plant root -- radiation exposure -- radiochemistry -- time -- toxicity testing -- water pollutant -- Americium -- Biomass -- Environmental Monitoring -- Hydrocharitaceae -- Hydrogen-Ion Concentration -- Plant Roots -- Plant Shoots -- Plants -- Radiochemistry -- Radioisotopes -- Time Factors -- Toxicity Tests -- Water -- Water Pollutants, Radioactive -- Elodea canadensis

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

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20.

System analysis of links interactions and development of ecosystems of different types / N. S. Pechurkin, I. M. Shirobokova // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1667-1674, DOI 10.1016/S0273-1177(03)80013-3 . - ISSN 0273-1177
Кл.слова (ненормированные):
Free energy -- Heuristic methods -- Hierarchical systems -- Mathematical models -- Photosynthesis -- Systems analysis -- Biological interactions -- Ecosystems -- anthropogenic effect -- ecosystem function -- systems analysis -- article -- biological model -- biomass -- ecology -- ecosystem -- energy transfer -- environmental protection -- food chain -- methodology -- microclimate -- plankton -- population dynamics -- statistics -- Biomass -- Conservation of Natural Resources -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Food Chain -- Models, Biological -- Plankton -- Population Dynamics
Аннотация: The anthropogenic impact on the Earth's ecosystems are leading to dramatic changes in ecosystem functioning and even to destruction of them. System analysis and the use of heuristic modeling can be an effective means to determine the main biological interactions and key factors that are of high importance for understanding the development of ecosystems. Cycling of limiting substances, induced by the external free energy flux, and trophic links interaction is the basis of the mathematical modeling studies presented in this paper. Mathematical models describe the dynamics of simplified ecosystems having different characteristics: 1) different degrees of biotic turnover closure (from open to completely closed); 2) different numbers of trophic links (including both "topdown", "bottom-up" regulation types); 3) different intensities of input - output flows of the limiting nutrient and its total amount in the system. Adaptive values of the changes of lower hierarchical levels (populational, trophic chain level) are to be estimated by integrity indices for total system functioning (e.g. NPP, total photosynthesis). The approach developed can be used for evaluating the contributions of lower hierarchical levels to the functioning of the higher hierarchical levels of the system. This approach may have value for determining biomanipulation management and their assessment. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Shirobokova, I.M.

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