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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 2049-2052. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): catalase--hydrogen peroxide--aerobic metabolism--article--bacterium--biomass--bioremediation--enzymology--metabolism--methodology--microbiology--sewage--waste management--water management--aerobiosis--bacteria--biodegradation, environmental--biomass--catalase--hydrogen peroxide--sewage--waste management--water microbiology--water purification
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Manukovsky N.S., Kovalev V.S., Somova L.A., Gurevich Yu.L., Sadovsky M.G.
Заглавие : Material balance and diet in bioregenerative life support systems: Connection with coefficient of closure
Место публикации : Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - С. 1563-1569. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.01.002
Ключевые слова (''Своб.индексиров.''): bioregenerative life support system--coefficient of closure--diet--soil-like substrate--additives--degradation--materials balance--nitrogen--nutrition--stoichiometry--bioregenerative life support systems--coefficient of closure--soil-like substrates--life support systems (spacecraft)--vegetable protein--biomass--conference paper--culture medium--diet--feces--growth, development and aging--human--methodology--microclimate--plant--urine--waste management--biomass--culture media--diet--ecological systems, closed--feces--humans--life support systems--plant proteins--plants, edible--urine--waste management
Аннотация: Bioregenerative life support systems (BLSS) with different coefficients of closure are considered. The 66.2% coefficient of closure achieved in "BIOS-3" facility experiments has been taken as a base value. The increase in coefficient of closure up to 72.6-93.0% is planned due to use of soil-like substrate (SLS) and concentrating of urine. Food values were estimated both in a base variant ("BIOS-3"), and with increases in the coefficient of closure. It is shown that food requirements will be more fully satisfied by internal crop production with an increase in the coefficient of closure of the BLSS. Changes of massflow rates on an 'input-output' and inside BLSS are considered. Equations of synthesis and degradation of organic substances in BLSS were examined using a stoichiometric model. The paper shows that at incomplete closure of BLSS containing SLS there is a problem of nitrogen balancing. To compensate for the removal of nitrogen from the system in urine and feces, it is necessary to introduce food and a nitrogen-containing additive. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kuznetsov A.M., Rodicheva E.K., Medvedeva S.E.
Заглавие : Analysis of river water by bioluminescent biotests
Место публикации : Luminescence. - 1999. - Vol. 14, Is. 5. - С. 263-265. - ISSN 15227235 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescent bioassay--pollution--water quality--fresh water--article--bioassay--escherichia coli--freeze drying--genetic procedures--luminescence--methodology--photobacterium--russian federation--sensitivity and specificity--water pollutant--biological assay--biosensing techniques--escherichia coli--freeze drying--fresh water--luminescence--photobacterium--russia--sensitivity and specificity--water pollutants, chemical
Аннотация: The bacterial bioluminescence has high sensitivity to the action of various inhibitors of biological activity. The lyophilized luminous bacteria Photobacterium phosphoreum (Microbiosensor B17 677F) and luminous strain Escherichia coli (Microbiosensor EC) from the Culture Collection IBSO were used to create bioluminescent biotests. They have been applied in ecological monitoring to determine the overall toxicity of the Yenisei and Angara Rivers and some water sources of Altai Territory. As a rule the heaviest pollution of water in studied rivers was registered near cities and settlements. The luminous bacteria biotests are simple and convenient in work, standardized and quantitative, have rapid response to actions of different substances and high sensitivity to environmental pollutants. It takes less than 30 min to do the biotest (the other biotests take 48-96 h). Copyright В© 1999 John Wiley & Sons, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Gitelson J., Terskov I., Sidko F.
Заглавие : Hydrogen bacteria as a potential regenerative LSS component and producer of ecologically clean degradable plastic.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 209-213. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--hydroxybutyric acid--polymer--air conditioning--article--biomass--catering service--chemistry--metabolism--methodology--microclimate--urine--waste management--water management--wautersia eutropha--air conditioning--biomass--carbon dioxide--cupriavidus necator--ecological systems, closed--food supply--hydroxybutyrates--life support systems--polymers--urine--waste management--water purification
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I.
Заглавие : Rubomycin microincapsulation with biodegradable polymer matrix
Место публикации : Antibiotiki i Khimioterapiya. - 2005. - Vol. 50, Is. 8-9. - С. 4-9. - ISSN 02352990 (ISSN)
Ключевые слова (''Своб.индексиров.''): daunorubicin (rubomycin)--microspheres--polyhydroxybutirate--antineoplastic antibiotic--daunorubicin--hydroxybutyric acid--microsphere--poly(3 hydroxybutyric acid)--poly-beta-hydroxybutyrate--polyester--polymer--article--chemistry--delayed release formulation--electronics--methodology--microcapsule--temperature--antibiotics, antineoplastic--capsules--daunorubicin--delayed-action preparations--hydroxybutyrates--microspheres--miniaturization--polyesters--polymers--temperature
Аннотация: A procedure for preparation of microspheres from biodegradable linear polyether of microbiological origin (polyhydroxybutirate, PHB) with using the technology of solvent evaporation was developed considering a specific example of two- and three-component emulsions. The procedure provided permanent preparation of the microspheres of high quality. The influence of the procedure (emulsion type, dispersion process and medium temperature) on the yield of the microspheres, their structure and size was shown. The temperature had a significant impact on incorporation of the antitumor anthracycline antibiotic daunorubicin (rubomycin) to the polymer matrix. The microspheres with various levels of the drug load (29 and 90% of the initial content in the emulsion) were prepared and the kinetics of the in vitro rubomycin release was studied. The dynamics of the highly toxic rubomycin release from the microspheres was on the whole even with the curve profile reaching the plateau in 20-22 hours of the observation period. The rate of the rubomycin release to the medium depended on the value of the antibiotic incorporation and was maximum within the first two hours (3.3 and 13.0 mcg/mlВ·h) that corresponded to the release of 0.97 and 3.89 of the incorporated antibiotic. The average rate of rubomycin release during 300 hours was 0.81В·10-4 and 2.3В·10-4 mcg/mlВ·h. The release constituted respectively 3.9 and 13.11% of the antibiotic incorporated to the microspheres.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S.
Заглавие : Management and control of microbial populations' development in LSS of missions of different durations
Место публикации : Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - С. 1621-1625. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2004.12.046
Ключевые слова (''Своб.индексиров.''): long-term missions--lss--macro- and microorganism--microbial populations--ecosystems--population statistics--probability--recycling--life support systems (lss)--long-term mission--macro-and microorganism--microbial populations--microorganisms--aerospace medicine--bioreactor--bioremediation--chlorella--conference paper--drug resistance--human--hygiene--immune system--instrumentation--methodology--microbiology--microclimate--physiology--space flight--waste management--weightlessness--aerospace medicine--biodegradation, environmental--bioreactors--chlorella--drug resistance--ecological systems, closed--environmental microbiology--humans--hygiene--immune system--life support systems--space flight--waste management--weightlessness
Аннотация: The problem of interaction between man and microorganisms in closed habitats is an inextricable part of the whole problem of co-existence between macro- and microorganisms. Concerning the support of human life in closed habitat, we can, conventionally, divide microorganisms, acting in life support system (LSS) into three groups: useful, neutral and harmful. The tasks, for human beings for optimal coexistence with microhabitants seem to be trivial: (1) to increase the activity of useful forms, (2) decrease the activity harmful forms, (3) not allow the neutral forms to become the harmful ones and even to help them to gain useful activity. The task of efficient management and control of microbial population's development in LSS highly depends on mission duration. As for short-term missions without recycling, the proper hygienic procedures are developed. For longer missions, the probability of transformation of the neutral forms into the harmful ones is becoming more dangerous. The LSS for long-term missions are to use cycling-recycling systems, including system with biological recycling. In these systems, microbial populations as regenerative link should be useful and active agents. Some problems of microbial populations control and management are discussed in the paper. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S., Pisman T.I.
Заглавие : Principles of biological adaptation of organisms in artificial ecosystems to changes of environmental factors
Место публикации : Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - С. 1512-1515. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2004.12.038
Ключевые слова (''Своб.индексиров.''): artificial ecosystems--biotic cycles--microorganisms--energy utilization--environmental impact--microorganisms--plants (botany)--purification--water--aes--artificial ecosystems--biotic cycles--material transformations--ecosystems--bioremediation--comparative study--conference paper--ecosystem--methodology--microbiology--microclimate--plant--sewage--waste management--water management--biodegradation, environmental--ecological systems, closed--ecosystem--environmental microbiology--life support systems--plants--sewage--soil microbiology--waste management--water purification
Аннотация: Studying material transformations and biotic cycling in artificial ecosystems (AES), we need to know the principles of biological adaptation of active organisms to change in the environment. Microorganisms in AES for water purification are the most active transforming organisms and consumers of the organic substances contained in wastes. Utilization of organic substances is directly connected with the energy fluxes used by AES. According to energy criteria, the energy fluxes used by a biological system tend to reach maximum values under stable conditions. Unutilized substrate concentration decreases as a result of biological adaptations. After a dramatic change in environmental factors, for example, after a sharp increase in the flow rate of organic substances, the biological system is not able to react quickly. The concentration of unutilized substrate increases and the energy flux used by the biological system decreases. The structure of the microbial community also changes, with a decrease in biological diversity. The efficiency of energy use by simple terrestrial ecosystems depends on the energetic intensity and interactions between plants and rhizospheric microorganisms. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Brilkov A.V., Loginov I.A., Morozova E.V., Pechurkin N.S.
Заглавие : Trends in microevolution of microbial populations in open systems
Место публикации : Doklady Biochemistry and Biophysics. - 2005. - Vol. 404, Is. 1-6. - С. 349-352. - ISSN 16076729 (ISSN) , DOI 10.1007/s10628-005-0111-x
Ключевые слова (''Своб.индексиров.''): article--bacterial phenomena and functions--bacterium--biological model--culture technique--escherichia coli--evolution--genetics--growth, development and aging--mathematics--methodology--mutation--nanotechnology--ph--physiology--population dynamics--time--bacteria--bacterial physiology--cell culture techniques--escherichia coli--evolution--hydrogen-ion concentration--mathematics--models, biological--mutation--nanotechnology--population dynamics--time factors
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Maryasova T.
Заглавие : Methodology of biospherics for theoretical sciences and practical use.
Место публикации : Life support & biosphere science : international journal of earth space. - 2000. - Vol. 7, Is. 2. - С. 219-224. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--biomass--ecosystem--energy metabolism--food chain--microclimate--theoretical model--biomass--ecological systems, closed--ecosystem--energy metabolism--food chain--life support systems--models, theoretical
Аннотация: This article deals with some methodological aspects of biospherics connected with theoretical sciences development and prospective use for practical application. Properties of experimental objects, methods and goals of biospherics as synthesising science have been discussed. The problem of stability of incomplete (natural and artificial) ecosystems has been considered. The concept of the ecosystem health based on effective functioning of different types of ecosystems has been developed.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Shirobokova I.M.
Заглавие : System analysis of links interactions and development of ecosystems of different types
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1667-1674. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)80013-3
Ключевые слова (''Своб.индексиров.''): free energy--heuristic methods--hierarchical systems--mathematical models--photosynthesis--systems analysis--biological interactions--ecosystems--anthropogenic effect--ecosystem function--systems analysis--article--biological model--biomass--ecology--ecosystem--energy transfer--environmental protection--food chain--methodology--microclimate--plankton--population dynamics--statistics--biomass--conservation of natural resources--ecological systems, closed--ecology--ecosystem--energy transfer--food chain--models, biological--plankton--population dynamics
Аннотация: The anthropogenic impact on the Earth's ecosystems are leading to dramatic changes in ecosystem functioning and even to destruction of them. System analysis and the use of heuristic modeling can be an effective means to determine the main biological interactions and key factors that are of high importance for understanding the development of ecosystems. Cycling of limiting substances, induced by the external free energy flux, and trophic links interaction is the basis of the mathematical modeling studies presented in this paper. Mathematical models describe the dynamics of simplified ecosystems having different characteristics: 1) different degrees of biotic turnover closure (from open to completely closed); 2) different numbers of trophic links (including both "topdown", "bottom-up" regulation types); 3) different intensities of input - output flows of the limiting nutrient and its total amount in the system. Adaptive values of the changes of lower hierarchical levels (populational, trophic chain level) are to be estimated by integrity indices for total system functioning (e.g. NPP, total photosynthesis). The approach developed can be used for evaluating the contributions of lower hierarchical levels to the functioning of the higher hierarchical levels of the system. This approach may have value for determining biomanipulation management and their assessment. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : Information capacity of symbol sequences
Колич.характеристики :13 с
Место публикации : Open Syst. Inf. Dyn.: KLUWER ACADEMIC PUBL, 2002. - Vol. 9, Is. 1. - P37-49. - ISSN 1230-1612, DOI 10.1023/A:1014230928565
Примечания : Cited References: 23
Предметные рубрики: REDUNDANCY
INTRONS
Аннотация: The information capacity of sequences is considered through the calculation of specific entropy of their frequency dictionary. The specific entropy was calculated against the reconstructed dictionary which bears the most probable continuations of shorter strings. The measure developed allows to distinguish the sequences both from the random ones, and those with high level of (rather simple) order. Some applications of the developed methodology to genetics, bioinformatics, and linguistics axe discussed.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GORBAN A.N., MIRKES E.M., POPOVA T.G., SADOVSKY M.G.
Заглавие : THE COMPARATIVE REDUNDANCY OF GENES OF VARIOUS ORGANISMS AND VIRUSES
Колич.характеристики :7 с
Место публикации : Genetika: MEZHDUNARODNAYA KNIGA, 1993. - Vol. 29, Is. 9. - P1413-1419. - ISSN 0016-6758
Примечания : Cited References: 20
Предметные рубрики: CODON
Аннотация: This paper is devoted to the comparative stude of redundancy of genetic texts of various organisms and viruses. To determine the tedundance of a gene, we have introduced the strict measure for that latter. The measure for a text's redundance is the length of restriction of Frequence/Correlation Dictionary of a given genetic text. Frequence/Correlation Dictionary is the ser of all subsequences belang to a given genetic text, accompanied by the frequencies of their occurrence. The restriction length is defined as that one, for which all the subsequences (of that length) are unique. We have found, that genes of human viruses are less redundant, in comparison to those of human genes. Other aspects of a comparative redundance invastigations of the genes are discussed. The problem of the determination of truet intron could be treated by this methodology, as well, as the evolution of genome.
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13.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Lankin J.P.
Заглавие : Adaptive simulation of atmospheric phenomena
Колич.характеристики :12 с
Место публикации : EIGHTH INTERNATIONAL SYMPOSIUM ON ATMOSPHERIC AND OCEAN OPTICS: ATMOSPHERIC PHYSICS. Ser. PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE): SPIE-INT SOC OPTICAL ENGINEERING, 2002. - Vol. 4678: 8th International Symposium on Atmospheric and Ocean Optics - Atmospheric Physics (JUN 25-29, 2001, IRKUTSK, RUSSIA). - P668-679. - ISBN 0277-786X, DOI 10.1117/12.458507. - ISBN 0-8194-4433-2
Примечания : Cited References: 46
Ключевые слова (''Своб.индексиров.''): atmosphere--system--adaptation--self-adaptation--neuroinformatics
Аннотация: The paper describes scientific methodology of developing complex nonlinear dynamic models which form the basis of a new scientific trend called Stikhioniks(1) and are a efficient tool to devise atmosphere models. The model is aimed to develop complexly organized hierarchical non-equilibrium adaptive systems with a wide range of connections and elements and is a new step as related to the now dominant mechanical-statistical paradigm.
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14.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Lankin J.P., Baskanova T.F.
Заглавие : Algorithms of self-adaptation for atmospheric model designing
Колич.характеристики :11 с
Место публикации : TENTH JOINT INTERNATIONAL SYMPOSIUM ON ATMOSPHERIC AND OCEAN OPTICS/ATMOSPHERIC PHYSICS, PT 2: LASER SENSING AND ATMOSPHERIC PHYSICS. Ser. PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE): SPIE-INT SOC OPTICAL ENGINEERING, 2004. - Vol. 5397: 10th Joint International Symposium on Atmospheric and Ocean Optics/Atmospheric Physics (JUN 24-28, 2003, Tomsk, RUSSIA). - P260-270. - ISBN 0277-786X, DOI 10.1117/12.548609. - ISBN 0-8194-5316-1
Примечания : Cited References: 23
Аннотация: The paper describes the principal limitations of the traditional methods used to construct atmospheric models. These limitations would not allow any fundamental improvement of atmospheric modeling. Ways are proposed to overcome the current limitations, based oil the methodology of constructing adaptive models and neuroinformatics. Algorithms of self-adaptation for neural networks intended for the construction of atmospheric models are given. Essentially, the developed algorithms are adaptive shells and can be easily transferred to other models.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : Comparison of real frequencies of strings vs. the expected ones reveals the information capacity of macromoleculae
Колич.характеристики :16 с
Место публикации : J. Biol. Phys.: SPRINGER, 2003. - Vol. 29, Is. 1. - P23-38. - ISSN 0092-0606, DOI 10.1023/A:1022554613105
Примечания : Cited References: 45
Предметные рубрики: DNA-SEQUENCE
ENTROPY
REDUNDANCY
Ключевые слова (''Своб.индексиров.''): dictionary--entropy--information capacity--markov model--ordered sequence--random sequence--reconstructed dictionary--specific entropy
Аннотация: The information capacity of nucleotide sequences is defined through the calculation of specific entropy of their frequency dictionary. The specific entropy of the frequency dictionary is calculated against the reconstructed dictionary; this latter bears the most probable continuations of the shorter strings. This developed measure allows to distinguish the sequences both from the randons ones, and from those with high level of (rather simple) order. Some implications of the developed methodology in the fields of genetics, bioinformatics, and molecular biology are discussed.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Terskov I.A., Gitelzon I.I., Sidko Ya. F.
Заглавие : Biological and nutritional value of the hydrogen bacteria biomass
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1984. - Vol. 20, Is. 4. - С. 540-550. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): alcaligenes eutrophus--diet--methodology--nonhuman--nutritional value
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Terskov I.A., Kovrov B.G., Lisovskii G.M., Okladnikov Yu.N., Sid'ko F.Ya., Trubachev I.N., Shilenko M.P., Alekseev S.S., Pan'kova I.M., Tirranen L.S.
Заглавие : Long-term experiments on man's stay in biological life-support system
Место публикации : Advances in Space Research. - 1989. - Vol. 9, Is. 8. - С. 65-71. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): agricultural products--ecosystems--food products--personnel - health--space flight - manned flight--bios-3 complex--phytotrons--spacecraft--water--air conditioning--article--biomass--crop--evaluation--growth, development and aging--human--instrumentation--light--metabolism--methodology--microclimate--photosynthesis--plant--waste management--wheat--air conditioning--biomass--crops, agricultural--ecological systems, closed--evaluation studies--humans--life support systems--light--photosynthesis--plants--triticum--waste management--water
Аннотация: We describe the experimental system having maximal possible closure of material recycling in an ecosystem, including people and plants, which was carried out in a hermetically sealed experimental complex "BIOS-3", 315 m2 in volume. The system included 2 experimentators and 3 phytotrons with plants (total sowing area of 63 m2). Plants were grown with round-the-clock lamp irradiation with 130 Wm-2 PAR intensity. The plants production was food for people. Water exchange of ecosystem, as well as gas exchange, was fully closed excluding liquids and gas samples taken for chemical analysis outside the system. The total closure of material turnover constituted 91%. Health state of the crew was estimated before, during and after the experiment. A 5-months period did not affect their health. The experiments carried out prove that the closed ecosystem of "man-plants" is a prototype of a life-support system for long-term space expeditions. В© 1989.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid'ko A.F., Shevyrnogov A.P.
Заглавие : Seasonal dependence of the spectral brightness of agricultural crops on plant chlorophyll content and physiological parameters
Место публикации : Earth Observ. Remote Sens.: HARWOOD ACAD PUBL GMBH, 2000. - Vol. 16, Is. 3. - С. 487-500. - 14. - ISSN 1024-5251
Примечания : Cited References: 18
Аннотация: The paper analyses the results of multi-year studies of the state of agricultural crops based on plant reflectance during the growth period. A universal methodology for determining the physiological state, and plant phytopigment content from the value of the chlorophyll potential S(t) in the region of the red absorption band lambda = 680 nm, which can be determined from the SEC values, is presented. It is shown that addition of mineral fertilisers (and, in particular, nitrogen) to soils, in on average 10 days slows the process of chlorophyll breakdown in phytocoenoses in comparison with control areas.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Titushin M.S., Feng Y.G., Lee J..., Vysotski E.S., Liu Z.J.
Заглавие : Protein-protein complexation in bioluminescence
Колич.характеристики :16 с
Место публикации : Protein Cell: HIGHER EDUCATION PRESS, 2011. - Vol. 2, Is. 12. - С. 957-972. - ISSN 1674-800X, DOI 10.1007/s13238-011-1118-y
Примечания : Cited References: 114. - The work was funded by "Fellowship for Young International Scientists" of Chinese Academy of Sciences. This work was supported by the National Natural Science Foundation of China (Grant Nos: 30870483, 31070660, 31021062 and 81072449), Ministry of Science and Technology of China (Nos. 2009DFB30310, 2009CB918803 and 2011CB911103), CAS Research Grants (Nos. YZ200839 and KSCX2-EW-J-3).
Предметные рубрики: GREEN-FLUORESCENT PROTEIN
LUCIFERIN-BINDING-PROTEIN
RENILLA-RENIFORMIS LUCIFERASE
VIBRIO-FISCHERI Y1
JELLYFISH CLYTIA-GREGARIA
ALPHA/BETA-HYDROLASE FOLD
AMINO-ACID-SEQUENCE
BACTERIAL LUCIFERASE
ENERGY-TRANSFER
CRYSTAL-STRUCTURE
Ключевые слова (''Своб.индексиров.''): green-fluorescent protein (gfp)--photoprotein--luciferase--lumazine protein--forster resonance energy transfer (fret)--docking
Аннотация: In this review we summarize the progress made towards understanding the role of protein-protein interactions in the function of various bioluminescence systems of marine organisms, including bacteria, jellyfish and soft corals, with particular focus on methodology used to detect and characterize these interactions. In some bioluminescence systems, protein-protein interactions involve an "accessory protein" whereby a stored substrate is efficiently delivered to the bioluminescent enzyme luciferase. Other types of complexation mediate energy transfer to an "antenna protein" altering the color and quantum yield of a bioluminescence reaction. Spatial structures of the complexes reveal an important role of electrostatic forces in governing the corresponding weak interactions and define the nature of the interaction surfaces. The most reliable structural model is available for the protein-protein complex of the Ca2+-regulated photoprotein clytin and green-fluorescent protein (GFP) from the jellyfish Clytia gregaria, solved by means of X-ray crystallography, NMR mapping and molecular docking. This provides an example of the potential strategies in studying the transient complexes involved in bioluminescence. It is emphasized that structural studies such as these can provide valuable insight into the detailed mechanism of bioluminescence.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lobova T.I., Barkhatov Y.V., Salamatina O.V., Popova L.Yu.
Заглавие : Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of Lake Shira as an indicator of human impact on the ecosystem
Место публикации : Microbiological Research. - 2008. - Vol. 163, Is. 2. - С. 152-160. - ISSN 09445013 (ISSN) , DOI 10.1016/j.micres.2006.03.014
Ключевые слова (''Своб.индексиров.''): antibiotic resistance--aquatic ecosystems--heterotrophic bacteria--human impact--monitoring--antibiotics--bacteria--ecosystems--environmental impact--mammals--antibiotic resistance--aquatic ecosystems--heterotrophic bacteria--materials--antiinfective agent--fresh water--allochthony--anthropogenic effect--antibiotic resistance--bacterium--concentration (composition)--heterotrophy--intertidal environment--monitoring--recreational facility--spring (season)--summer--animal--article--bacterial count--bacterium--drug effect--ecosystem--environmental monitoring--heterotrophy--human--isolation and purification--methodology--microbiological examination--microbiology--multidrug resistance--russian federation--season--animals--anti-bacterial agents--bacteria--colony count, microbial--drug resistance, multiple, bacterial--ecosystem--environmental monitoring--fresh water--heterotrophic processes--humans--microbial sensitivity tests--russia--seasons--eurasia--khakassia--lake shira--russian federation--animalia--bacteria (microorganisms)
Аннотация: Resistance to Ampicillin and Kanamycin displayed by heterotrophic bacteria isolated in Summer and in Spring from the littoral and the central parts of Lake Shira (a therapeutic lake in the Khakasia Republic, Russia) has been investigated. It has been found that in Summer, human and animal microflora featuring multiple antibiotic resistance (to Ampicillin and Kanamycin) predominates in all the studied stations of the littoral zone of the lake. In Spring, concentrations of bacteria featuring multiple antibiotic resistance decrease significantly and bacteria sensitive to antibiotics predominate in the lake. Emergence of multiple antibiotic resistance in bacteria of Lake Shira is caused by the input of allochthonous bacteria into the lake; this feature of heterotrophic bacteria of Lake Shira can be used to monitor the impact on the ecosystem made by health resorts. В© 2006 Elsevier GmbH. All rights reserved.
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