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Methanolobus halotolerans sp. nov., isolated from the saline Lake Tus in Siberia / Y. Shen, S. C. Chen, M. C. Lai [et al.] // Int. J. Syst. Evol. Microbiol. - 2020. - Vol. 70, Is. 10. - P5586-5593, DOI 10.1099/ijsem.0.004453. - Cited References:38. - This work was supported by grants from the Ministry of Education, Taiwan, ROC under the ATU plan (to M.C.L.), by the thematic project funding of Taiwan-Russian Joint Project (NSC99-2923-B-001-001-MY3 and NSC102-2923-B-001-004 to S.L.T.), by the Ministry of Science and Technology (MOST103/104/105/106/107-3113-M-005-001 to M.C.L.), Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a (to D.Y.R. and A.G.D.). . - ISSN 1466-5026. - ISSN 1466-5034

РУБ Microbiology
Рубрики:
TAIWANENSIS SP NOV.
   METHYLOTROPHIC METHANOGEN
   SEDIMENTS
   ACCURATE
   DNA
Аннотация: A halotolerant, psychrotolerant and methylotrophic methanogen, strain SY-01(T), was isolated from the saline Lake Tus in Siberia. Cells of strain SY-01(T) were non-motile, cocci and 0.8-1.0 mu m in diameter. The only methanogenic substrate utilized by strain SY-01(T) was methanol. The temperature range of growth for strain SY-01(T) was from 4 to 40 degrees C and the optimal temperature for growth was 30 degrees C. The pH range of growth was from pH 7.2 to 9.0, with optimal growth at pH 8.0. The NaCl range of growth was 0-1.55 M with optimal growth at 0.51 M NaCl. The G+C content of the genome of strain SY-01(T) was 43.6 mol% as determined by genome sequencing. Phylogenetic analysis revealed that strain SY-01(T) was most closely related to Methanolobus zinderi SD1(T) (97.3% 16S rRNA gene sequence similarity), and had 95.5-97.2 % similarities to other Methanolobus species with valid names. Genome relatedness between strain SY-01(T) and DSM 21339(T) was computed using average nucleotide identity and digital DNA-DNA-hybridization, which yielded values of 79.7 and 21.7 %, respectively. Based on morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain SY-01(T) represents a novel species of the genus Methanolobus, and the name Methanolobus halotolerans sp. nov. is proposed. The type strain is SY-01(T) (=BCRC AR10051(T)=NBRC 113166(T)=DSM 107642(T)).

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Держатели документа:
Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan.
Sanming Univ, Sch Resources & Chem Engn, Sanming 365004, Fujian, Peoples R China.
Sanming Univ, Fujian Prov Key Lab Resources & Environm Monitori, Sanming 365004, Fujian, Peoples R China.
Natl Chung Hsing Univ, Agr Biotechnol Ctr, Taichung, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Shen, Y.a.; Chen, Sheng-Chung; Lai, Mei-Chin; Huang, Hsing-Hua; Chiu, Hsiu-Hui; Tang, Sen-Lin; Rogozin, Denis Yu; Degermendzhy, Andrey G.; Ministry of Education, Taiwan, ROC under the ATU planMinistry of Education, Taiwan; thematic project funding of Taiwan-Russian Joint Project [NSC99-2923-B-001-001-MY3, NSC102-2923-B-001-004]; Ministry of Science and Technology, TaiwanMinistry of Science and Technology, Taiwan [MOST103/104/105/106/107-3113-M-005-001]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [14-04-01060-a]

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Recombinant Ca2+-regulated photoproteins of ctenophores: current knowledge and application prospects / L. P. Burakova, E. S. Vysotski // Appl. Microbiol. Biotechnol. - 2019. - Vol. 103, Is. 15. - P5929-5946, DOI 10.1007/s00253-019-09939-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Intracellular calcium -- Photoinactivation -- Absorption spectroscopy -- Alkalinity -- Animals -- Binding sites -- Cloning -- Encoding (symbols) -- Phosphorescence -- Physicochemical properties -- Signal encoding -- Amino acid sequence -- Application prospect -- Biotechnology applications -- Coelenterazine -- Intracellular calcium -- Marine animals -- Photoinactivation -- Structural feature -- Bioluminescence -- Animalia -- Cnidaria -- Ctenophora (coelenterates)
Аннотация: Bright bioluminescence of ctenophores is conditioned by Ca2+-regulated photoproteins. Although they share many properties characteristic of hydromedusan Ca2+-regulated photoproteins responsible for light emission of marine animals belonging to phylum Cnidaria, a substantial distinction still exists. The ctenophore photoproteins appeared to be extremely sensitive to light—they lose the ability for bioluminescence on exposure to light over the entire absorption spectrum. Inactivation is irreversible because keeping the inactivated photoprotein in the dark does not recover its activity. The capability to emit light can be restored only by incubation of inactivated photoprotein with coelenterazine in the dark at alkaline pH in the presence of oxygen. Although these photoproteins were discovered many years ago, only the cloning of cDNAs encoding these unique bioluminescent proteins in the early 2000s has provided a new impetus for their studies. To date, cDNAs encoding Ca2+-regulated photoproteins from four different species of luminous ctenophores have been cloned. The amino acid sequences of ctenophore photoproteins turned out to completely differ from those of hydromedusan photoproteins (identity less than 29%) though also similar to them having three EF-hand Ca2+-binding sites. At the same time, these photoproteins reveal the same two-domain scaffold characteristic of hydromedusan photoproteins. This review is an attempt to systemize and critically evaluate the data scattered through various articles regarding the structural features of recombinant light-sensitive Ca2+-regulated photoproteins of ctenophores and their bioluminescent and physicochemical properties as well as to compare them with those of hydromedusan photoproteins. In addition, we also discuss the prospects of their biotechnology applications. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Burakova, L. P.; Vysotski, E. S.

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Effects of zooplankton carcasses degradation on freshwater bacterial community composition and implications for carbon cycling / O. V. Kolmakova [et al.] // Environ. Microbiol. - 2018, DOI 10.1111/1462-2920.14418 . - Article in press. - ISSN 1462-2912
Аннотация: Non-predatory mortality of zooplankton provides an abundant, yet, little studied source of high quality labile organic matter (LOM) in aquatic ecosystems. Using laboratory microcosms, we followed the decomposition of organic carbon of fresh 13C-labelled Daphnia carcasses by natural bacterioplankton. The experimental setup comprised blank microcosms, that is, artificial lake water without any organic matter additions (B), and microcosms either amended with natural humic matter (H), fresh Daphnia carcasses (D) or both, that is, humic matter and Daphnia carcasses (HD). Most of the carcass carbon was consumed and respired by the bacterial community within 15 days of incubation. A shift in the bacterial community composition shaped by labile carcass carbon and by humic matter was observed. Nevertheless, we did not observe a quantitative change in humic matter degradation by heterotrophic bacteria in the presence of LOM derived from carcasses. However, carcasses were the main factor driving the bacterial community composition suggesting that the presence of large quantities of dead zooplankton might affect the carbon cycling in aquatic ecosystems. Our results imply that organic matter derived from zooplankton carcasses is efficiently remineralized by a highly specific bacterial community, but does not interfere with the bacterial turnover of more refractory humic matter. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Siberian Federal University, Institute of Fundamental Biology and Biotechnology, Krasnoyarsk, Russian Federation
Department of Experimental Limnology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, Germany
GFZ German Research Centre for Geosciencess, Section 5.3 Geomicrobiology, Potsdam, Germany
Experimental Phycology and Culture Collection of Algae (SAG), University of Gottingen, Gottingen, Germany
Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany

Доп.точки доступа:
Kolmakova, O. V.; Gladyshev, M. I.; Fonvielle, J. A.; Ganzert, L.; Hornick, T.; Grossart, H. -P.

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Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production / T. Volova [et al.] // Appl. Microbiol. Biotechnol. - 2018, DOI 10.1007/s00253-018-9460-0 . - Article in press. - ISSN 0175-7598
Кл.слова (ненормированные):
Glycerol -- Polyhydroxyalkanoates -- Productivity -- Properties -- Synthesis -- Batch cell culture -- Fermentation -- Organic polymers -- Pilot plants -- Productivity -- Purification -- Synthesis (chemical) -- Degree of crystallinity -- Fed-batch fermentation -- Poly-3-hydroxybutyrate -- Poly-hydroxyalkanoate -- Polyhydroxyalkanoates -- Properties -- Temperature characteristic -- Volumetric productivity -- Glycerol
Аннотация: The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Ave, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Volova, T.; Demidenko, A.; Kiselev, E.; Baranovskiy, S.; Shishatskaya, E.; Zhila, N.

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Comprehensive Insights Into Composition, Metabolic Potentials, and Interactions Among Archaeal, Bacterial, and Viral Assemblages in Meromictic Lake Shunet in Siberia / Y. T. Wu [et al.] // Front. Microbiol. - 2018. - Vol. 9. - Ст. 1763, DOI 10.3389/fmicb.2018.01763. - Cited References:103. - This research was supported by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC 99-2923-B-001-001-MY3 and NSC 102-2923-B-001-004 and the Russian Foundation for Basic Research, Grant No. 16-05-00091-a. The research was partially supported by the Council on grants from the President of the Russian Federation for support of leading scientific schools (grant NSh-9249.2016.5). . - ISSN 1664-302X

РУБ Microbiology
Рубрики:
BACTERIOPLANKTON COMMUNITY COMPOSITION
RIBOSOMAL-RNA GENES
ANTARCTIC
Кл.слова (ненормированные):
Lake Shunet -- metagenomics -- archaeal -- bacterial and viral assemblages -- meromictic lake
Аннотация: Microorganisms are critical to maintaining stratified biogeochemical characteristics in meromictic lakes; however, their community composition and potential roles in nutrient cycling are not thoroughly described. Both metagenomics and metaviromics were used to determine the composition and capacity of archaea, bacteria, and viruses along the water column in the landlocked meromictic Lake Shunet in Siberia. Deep sequencing of 265 Gb and high-quality assembly revealed a near-complete genome corresponding to Nonlabens sp. sh3vir. in a viral sample and 38 bacterial bins (0.2-5.3 Mb each). The mixolimnion (3.0 m) had the most diverse archaeal, bacterial, and viral communities, followed by the monimolimnion (5.5 m) and chemocline (5.0 m). The bacterial and archaeal communities were dominated by Thiocapsa and Methanococcoides, respectively, whereas the viral community was dominated by Siphoviridae. The archaeal and bacterial assemblages and the associated energy metabolism were significantly related to the various depths, in accordance with the stratification of physicochemical parameters. Reconstructed elemental nutrient cycles of the three layers were interconnected, including co-occurrence of denitrification and nitrogen fixation in each layer and involved unique processes due to specific biogeochemical properties at the respective depths. According to the gene annotation, several pre-dominant yet unknown and uncultured bacteria also play potentially important roles in nutrient cycling. Reciprocal BLAST analysis revealed that the viruses were specific to the host archaea and bacteria in the mixolimnion. This study provides insights into the bacterial, archaeal, and viral assemblages and the corresponding capacity potentials in Lake Shunet, one of the three meromictic lakes in central Asia. Lake Shunet was determined to harbor specific and diverse viral, bacterial, and archaeal communities that intimately interacted, revealing patterns shaped by indigenous physicochemical parameters.

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Держатели документа:
Natl Pingtung Univ Sci & Technol, Dept Forestry, Neipu, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan.
Germark Biotechnol Co Ltd, Taichung, Taiwan.
Univ Melbourne, Melbourne Sch Engn, Optimisat & Pattern Recognit Grp, Melbourne, Vic, Australia.
Acad Sinica, Taiwan Int Grad Program, Mol & Biol Agr Sci, Taipei, Taiwan.
Natl Chung Hsing Univ, Grad Inst Biotechnol, Dept Life Sci, Taichung, Taiwan.
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Wu, Yu-Ting; Yang, Cheng-Yu; Chiang, Pei-Wen; Tseng, Ching-Hung; Chiu, Hsiu-Hui; Saeed, Isaam; Baatar, Bayanmunkh; Rogozin, Denis; Halgamuge, Saman; Degermendzhi, Andrei; Tang, Sen-Lin; Ministry of Science and Technology in Taiwan [NSC 99-2923-B-001-001-MY3, NSC 102-2923-B-001-004]; Russian Foundation for Basic Research [16-05-00091-a]; Russian Federation [NSh-9249.2016.5]

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Structure of microbial communities of peat soils in two bogs in Siberian tundra and forest zones / I. D. Grodnitskaya [et al.] // Microbiology. - 2018. - Vol. 87, Is. 1. - P89-102, DOI 10.1134/S0026261718010083 . - ISSN 0026-2617
Кл.слова (ненормированные):
16S rRNA gene -- bacterial diversity -- CH4 and CO2 emission -- cryogenic conditions -- methanogenesis -- methanotrophy -- microbial biomass and chemoorganotroph respiration -- oligo-mesotrophic and polygonal bogs -- permafrost -- subarctic tundra
Аннотация: The structure and functional activity of microbial complexes of a forest oligo-mesotrophic subshrub- grass-moss bog (OMB, Central Evenkiya) and a subshrub-sedge bog in the polygonal tundra (PB, Lena River Delta Samoylovsky Island) was studied. Soil of the forest bog (OMB) differed from that of the polygonal tundra bog (PB) in higher productivity (Corg, Ntotal, P, and K reserves), higher biomass of aerobic chemoorganotrophs (2.0 to 2.6 times), and twice the level of available organic matter. The contribution of microorganisms to the carbon pool was different, with the share of Cmic in Corg 1.4 to 2.5 times higher in PB compared to OMB. Qualitative composition of the methane cycle microorganisms in PB and OMB soils differed significantly. Methanogenic archaea (Euryarchaeota) in the shrub-sedge PB of tundra were more numerous and diverse than in the oligo-mesotrophic bog (OMB) and belonged to six families (Methanomassiliicoccaceae, Methanoregulaceae, Methanobacteriaceae, Methanomicrobiaceaee, Methanosarcinaceae, and Methanotrichaceae), while members of only four families (Methanosarcinacea, Methanobacteriaceae, Methanotrichaceae, and Methanomassiliicoccaceae) were revealed in OMB. In both bogs, methane-oxidizing bacteria belonged to Alphaproteobacteria (II) and Gammaproteobacteria (I). Methanotroph diversity was higher in OMB than in PB. Microbial communities of PB soils had higher potential activity of methanogenesis and methanotrophy compared to those of OMB. Methanogenic and methanotrophic activities in PB were 20 and 2.3 times higher, respectively, than in OMB. © 2018, Pleiades Publishing, Ltd.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Information and Methodical Center for Expertise, Accounting, and Analysis of Rotation of Medical Agents, Kranoyarsk, Russian Federation
Roche Diagnostika Rus, Moscow, Russian Federation

Доп.точки доступа:
Grodnitskaya, I. D.; Trusova, M. Y.; Syrtsov, S. N.; Koroban, N. V.

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Methanolobus psychrotolerans sp. nov., a psychrotolerant methanoarchaeon isolated from a saline meromictic lake in siberia / S. -C. Chen [et al.] // Int. J. Syst. Evol. Microbiol. - 2018. - Vol. 68, Is. 4. - P1378-1383, DOI 10.1099/ijsem.0.002685 . - ISSN 1466-5026
Кл.слова (ненормированные):
Archaea -- Lake shira -- Methanogen -- Methanolobus -- Psychrotolerant -- Saline meromictic lake
Аннотация: A psychrotolerant, methylotrophic methanogen, strain YSF-03T, was isolated from the saline meromictic Lake Shira in Siberia. Cells of strain YSF-03T were non-motile, irregular cocci and 0.8–1.2?m in diameter. The methanogenic substrates utilized by strain YSF-03T were methanol and trimethylamine. The temperature range of growth for strain YSF-03T was from 0 to 37 °C. The optimum growth conditions were 30–37 °C, pH 7.0–7.4 and 0.17M NaCl. The G+C content of the genome of strain YSF-03T was 41.3 mol%. Phylogenetic analysis revealed that strain YSF-03T was most closely related to Methanolobus profundi MobMT (98.15% similarity in 16S rRNA gene sequence). Genome relatedness between strain YSF-03T and MobMT was computed using the Genome-to-Genome Distance Calculator and average nucleotide identity, which gave values of 23.5 and 79.3 %, respectively. Based on the morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain YSF-03T represents a novel species of the genus Methanolobus, for which the name Methanolobus psychrotolerans sp. nov. is proposed. The type strain is YSF-03T (=BCRC AR10049T=DSM 104044T=NBRC 112514T). © 2018 IUMS.

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Держатели документа:
Department of Life Science, National Chung Hsing University, Taiwan
Agricultural Biotechnology Center, National Chung Hsing University, Taiwan
Biodiversity Research Center, Academia Sinica, Taiwan
Institute of Biophysics Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Chen, S. -C.; Huang, H. -H.; Lai, M. -C.; Weng, C. -Y.; Chiu, H. -H.; Tang, S. -L.; Rogozin, D. Y.; Degermendzhy, A. G.

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Production and properties of bacterial cellulose by the strain Komagataeibacter xylinus B-12068 / T. G. Volova [et al.] // Appl. Microbiol. Biotechnol. - 2018. - P1-12, DOI 10.1007/s00253-018-9198-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
Bacterial cellulose -- Growth conditions -- Komagataeibacter xylinus -- Biocompatibility -- Cell culture -- Cellulose -- Cultivation -- Glucose -- 3t3 mouse fibroblasts -- Bacterial cellulose -- Cultivation conditions -- Emission spectrometry -- Ethanol concentrations -- Growth conditions -- Komagataeibacter xylinus -- Physical and mechanical properties -- Substrates
Аннотация: A strain of acetic acid bacteria, Komagataeibacter xylinus B-12068, was studied as a source for bacterial cellulose (BC) production. The effects of cultivation conditions (carbon sources, temperature, and pH) on BC production and properties were studied in surface and submerged cultures. Glucose was found to be the best substrate for BC production among the sugars tested; ethanol concentration of 3% (w/v) enhanced the productivity of BC. Optimization of medium and cultivation conditions ensures a high production of BC on glucose and glycerol, up to 2.4 and 3.3 g/L/day, respectively. C/N elemental analysis, emission spectrometry, SEM, DTA, and X-ray were used to investigate the structure and physical and mechanical properties of the BC produced under different conditions. MTT assay and SEM showed that native cellulose membrane did not cause cytotoxicity upon direct contact with NIH 3T3 mouse fibroblast cells and was highly biocompatible. © 2018 Springer-Verlag GmbH Germany, part of Springer Nature

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Держатели документа:
Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Siberian Federal University, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Prudnikova, S. V.; Sukovatyi, A. G.; Shishatskaya, E. I.

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Analytical Enzymatic Reactions in Microfluidic Chips / K. A. Lukyanenko [et al.] // Appl. Biochem. Microbiol. - 2017. - Vol. 53, Is. 7. - P775-780, DOI 10.1134/S0003683817070043. - Cited References:15. - The study was supported by a grant from the Russian Science Foundation (project No. 15-19-10041). . - ISSN 0003-6838. - ISSN 1573-8183

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
BIOAVAILABLE HEAVY-METALS
   DEVICES
   POINT
   LAB
Кл.слова (ненормированные):
bioluminescence -- luciferase -- microfluidics -- microfluidic chip -- enzymatic -- bioassay
Аннотация: A number of approaches have been proposed and tested to transfer enzymatic reactions into the functional elements of microfluidic chips on the example of the bienzyme bioluminescent reaction involving NAD(P)H:FMN-oxidoreductase and luciferase. Measurement of the catalytic activity of these enzymes (under the influence of pollutants) is the basis of enzymatic bioassay of various liquids. It was found that all of the components of the reaction must be placed in the same cell of the chip to improve the reproducibility of the measurements. The use of starch gel as a carrier for immobilization and gelatin as a scaffold in the reactor of the chip enables the preservation of enzyme activity in the course of sealing the chip at room temperature. It is shown that the components of the reaction should be vigorously stirred in a microfluidic chip reactor to improve the efficiency of the analysis. As a result of the studies, a prototype of microfluidic chip based on the enzymatic bioluminescent reaction is proposed. It is characterized by a detection limit of copper sulfate of 3 mu M that corresponds to the sensitivity of traditional lux-biosensors based on living cells. The analysis time is reduced to 1 min, and the analysis can be performed by individuals without special laboratory skills.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
St Petersburg Inst Fine Mech & Opt, St Petersburg 197101, Russia.
Inst Analyt Instrumentat, St Petersburg 198095, Russia.

Доп.точки доступа:
Lukyanenko, K. A.; Denisov, I. A.; Yakimov, A. S.; Esimbekova, E. N.; Belousov, K. I.; Bukatin, A. S.; Kukhtevich, I. V.; Sorokin, V. V.; Evstrapov, A. A.; Belobrov, P. I.; Russian Science Foundation [15-19-10041]

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10.

Mercury, selenium and fish oils in marine food webs and implications for human health / M. O. Gribble [et al.] // J. Mar. Biol. Assoc. U.K. - 2016. - Vol. 96, Is. 1. - P43-59, DOI 10.1017/S0025315415001356 . - ISSN 0025-3154
Кл.слова (ненормированные):
Docosahexaenoic acid -- Ecotoxicology -- Eicosapentaenoic acid -- Fish oils -- Mercury -- N-3 fatty acids -- Oceans and human health -- OHH -- Public health -- Selenium
Аннотация: Humans who eat fish are exposed to mixtures of healthful nutrients and harmful contaminants that are influenced by environmental and ecological factors. Marine fisheries are composed of a multitude of species with varying life histories, and harvested in oceans, coastal waters and estuaries where environmental and ecological conditions determine fish exposure to both nutrients and contaminants. Many of these nutrients and contaminants are thought to influence similar health outcomes (i.e., neurological, cardiovascular, immunological systems). Therefore, our understanding of the risks and benefits of consuming seafood require balanced assessments of contaminants and nutrients found in fish and shellfish. In this paper, we review some of the reported benefits of fish consumption with a focus on the potential hazards of mercury exposure, and compare the environmental variability of fish oils, selenium and mercury in fish. A major scientific gap identified is that fish tissue concentrations are rarely measured for both contaminants and nutrients across a range of species and geographic regions. Interpreting the implications of seafood for human health will require a better understanding of these multiple exposures, particularly as environmental conditions in the oceans change. © Marine Biological Association of the United Kingdom, 2015.

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Держатели документа:
Department of Preventive Medicine, University of Southern California, Keck School of Medicine, Los Angeles, CA, United States
School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, United States
Department of Environmental Health Sciences, University at Albany School of Public Health, State University of New York, Rensselaer, NY, United States
Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, United States
Department of Veterinary Medicine, College of Natural Science and Mathematics, University of Alaska Fairbanks, Fairbanks, AK, United States
Institute of Biophysics of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Department of Biological Sciences, Dartmouth College, Hanover, NH, United States

Доп.точки доступа:
Gribble, M. O.; Karimi, R.; Feingold, B. J.; Nyland, J. F.; O'Hara, T. M.; Gladyshev, M. I.; Chen, C. Y.

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11.

Dynamics of purple sulfur bacteria in a meromictic saline Lake Shunet (Khakassia, Siberia) in 2007–2013 / D. Y. Rogozin, V. V. Zykov, M. O. Tarnovskii // Microbiology. - 2016. - Vol. 85, Is. 1. - P93-101, DOI 10.1134/S0026261716010100 . - ISSN 0026-2617
Кл.слова (ненормированные):
long-term dynamics -- meromictic lake -- purple sulfur bacteria -- stratification
Аннотация: According to the results of seasonal monitoring, in 2007–2013 purple sulfur bacteria morphologically similar to Thiocapsa sp. Shira_1 (AJ633676 in EMBL/GenBank) predominated in the anoxygenic phototrophic community of the water column of the meromictic Lake Shira (Khakassia, Siberia). No pronounced seasonal periodicity in the total cell number in the water column was revealed during the period of observation. In some years cell number during the period when the lake was covered with ice was reliably higher than in summer. The absence of seasonal periodicity was probably due to the low amplitude of seasonal variations in temperature and illumination in the redox zone, resulting from its relatively deep location (12–16 m). The year-to-year dynamics was characterized by a reliable decrease of the total cell number in 2009–2010 and maxima in 2007 and 2011–2012. Canonical correlation analysis revealed that water temperature in the redox zone was the best predictor of the PSB abundance in Lake Shira. Water temperature, in turn, depended on the depth of mixing of the water column. Intense mixing in 2009–2011 was probably responsible for decreased PSB abundance in the lake. On the other hand, the absence of deep winter mixing, resulting in stable conditions in the chemocline, favored the preservation of relatively high PSB biomass. Prediction of circulation depth, which depends mainly on the weather conditions and dynamics of the water level, is required for prediction of PSB abundance in Lake Shira. These results may be useful for paleolimnological reconstructions of the history of the lake based on the remnants of purple sulfur bacteria in bottom sediments. © 2016, Pleiades Publishing, Ltd.

Scopus,
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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Rogozin, D. Y.; Zykov, V. V.; Tarnovskii, M. O.

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12.

Total peroxidase and catalase activity of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in comparison with the level of light emission [Text] / O. A. Mogil'naya [et al.] // Appl. Biochem. Microbiol. - 2015. - Vol. 51, Is. 4. - P419-424, DOI 10.1134/S0003683815040110. - Cited References:35. - The authors are grateful to N. V. Psurtseva (curator of the collection of basidiomycetes of the Botanical Institute, Russian Academy of Science) for help with the species affiliation of the IBSO 2328 culture. This work was supported by the Program of Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences, project no. 71. . - ISSN 0003-6838. - ISSN 1573-8183

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
OXIDATIVE STRESS
   SYSTEM
   FUNGI
   BIOLUMINESCENCE
   LUMINESCENCE
Кл.слова (ненормированные):
basidiomycetes -- luminescence -- peroxidase -- catalase
Аннотация: The peroxidase and catalase activities in the mycelium of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in normal conditions and under stress were compared. An increase in the luminescence level was observed under stress, as well as an increase in peroxidase and catalase activities. Moreover, the peroxidase activity in extracts of A. borealis mycelium was found to be almost one and a half orders of magnitude lower, and the catalase activity more than two orders of magnitude higher in comparison with the N. nambi mycelium. It can be suggested that the difference between the brightly luminescent and dimly luminescent mycelium of N. nambi is due to the content of (HO2)-O-2 or other peroxide compounds.

WOS,
Scopus
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Mogil'naya, O. A.; Ronzhin, N. O.; Medvedeva, S. E.; Bondar', V. S.; Siberian Branch of the Russian Academy of Sciences [71]

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13.

Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium Seliberia carboxydohydrogena Z-1062 [Text] / T. Volova, N. Zhila, E. Shishatskaya // J. Ind. Microbiol. Biotechnol. - 2015. - Vol. 42, Is. 10. - P1377-1387, DOI 10.1007/s10295-015-1659-9. - Cited References:36. - The research was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 1367-5435. - ISSN 1476-5535

РУБ Biotechnology & Applied Microbiology
Рубрики:
CARBON-MONOXIDE
   RALSTONIA-EUTROPHA
   POLYHYDROXYALKANOATES
   GROWTH
   H-2
Кл.слова (ненормированные):
Carboxydobacteria -- Synthesis -- Poly(3-hydroxybutyrate) -- Carbon monoxide
Аннотация: The present study addresses growth parameters and physiological and biochemical characteristics of the aerobic CO-oxidizing carboxydobacterium Seliberia carboxydohydrogena Z-1062. Poly(3-hydroxybutyrate) yields were investigated in experiments with limiting concentrations of mineral nutrients (nitrogen or sulfur or nitrogen and sulfur) in batch culture of S. carboxydohydrogena Z-1062 grown on gas mixtures consisting of CO2, O-2, H-2, and CO. CO concentrations of 10, 20, and 30 % v/v did not affect polymer synthesis, whose content after 56-h cultivation under limiting concentrations of nitrogen and sulfur was 52.6-62.8 % of biomass weight at a productivity of 0.13-0.22 g/L h. The inhibitory effect of CO on cell concentration was revealed at CO concentration of 30 % v/v. That also caused a decrease in substrate (H-2 and O-2) use efficiency. Thus, this carboxydobacterium can be regarded as a potential producer of polyhydroxyalkanoates from industrial hydrogenous sources.

WOS,
Scopus
Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Volova, Tatiana; Zhila, Natalia; Shishatskaya, Ekaterina; Russian Academy of Sciences [01201351505]

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14.

Aliidiomarina shirensis sp. nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina [] / H. -H. Chiu [et al.] // Int. J. Syst. Evol. Microbiol. - 2014. - Vol. 64, Is. PART 4. - Ст. 057851. - P1334-1339, DOI 10.1099/ijs.0.057851-0 . - ISSN 1466-5026
Аннотация: Strain AIST, an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Aliidiomarina sanyensis', Idiomarina maris and AIST formed a distinct lineage. The sequence similarities between AIST and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIST was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C17: 0, iso-C15: 0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIST represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIST (= JCM 17761T = BCRC 80327T). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina maris be transferred to the genus Aliidiomarina. © 2014 IUMS.

Scopus
Держатели документа:
Biodiversity Research Center, Academia Sinica, Taipei, Taiwan
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Chiu, H.-H.; Rogozin, D.Y.; Huang, S.-P.; Degermendzhy, A.G.; Shieh, W.Y.; Tang, S.-L.

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15.

Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing [Text] / O. V. Kolmakova [et al.] // FEMS Microbiol. Ecol. - 2014. - Vol. 89, Is. 2. - P442-450, DOI 10.1111/1574-6941.12355. - Cited References: 36. - This work was supported by the Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation, agreement 11.G34.31.0014, and by the project G-1 of Siberian Federal University, carried out according to Federal tasks of the Ministry of Education and Science of Russian Federation. . - ISSN 0168-6496. - ISSN 1574-6941

РУБ Microbiology
Рубрики:
DISSOLVED ORGANIC-MATTER
   INLAND WATERS
   CARBON
   BACTERIOPLANKTON
   COMMUNITY
   GREENGENES
   ECOSYSTEM
   RESERVOIR
   PATTERNS
   PRIMERS
Кл.слова (ненормированные):
bacterial community -- diversity -- 16S rRNA gene -- Yenisei River
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section.

WOS
Держатели документа:
[Kolmakova, Olesya V.
Gladyshev, Michail I.] Siberian Fed Univ, Krasnoyarsk, Russia
[Kolmakova, Olesya V.
Gladyshev, Michail I.
Trusova, Maria Y.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Rozanov, Alexey S.
Peltek, Sergey E.] Russian Acad Sci, Inst Cytol & Genet, Siberian Branch, Novosibirsk 630090, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kolmakova, O.V.; Gladyshev, M.I.; Rozanov, A.S.; Peltek, S.E.; Trusova, M.Y.; Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation [11.G34.31.0014]; Siberian Federal University

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16.

Aliidiomarina shirensis sp nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina [Text] / H. H. Chiu [et al.] // Int. J. Syst. Evol. Microbiol. - 2014. - Vol. 64. - P1334-1339, DOI 10.1099/ijs.0.057851-0. - Cited References: 22. - We thank Dr Egor S. Zadereev and Dr Vladimir V. Zykov at the Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, for assistance with sampling. This study was supported by Russia Taiwan joint project funding (NSC 99-2923-B-001-001-MY3) from the National Science Council, Taiwan, the Russian Foundation for Basic Research, Grant No. 14-04-01060-a and Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project No. 11. . - ISSN 1466-5026. - ISSN 1466-5034

РУБ Microbiology
Рубрики:
SHALLOW COASTAL WATER
   RIBOSOMAL-RNA GENE
   EMENDED DESCRIPTION
   PSEUDIDIOMARINA
   PHYLOTYPES
   SEQUENCE
   TAIWAN
Аннотация: Strain AIS(T), an aerobic halophilic, Gram-reaction-negative, heterotrophic bacterium isolated from the water of Shira Lake in Khakasia, southern Siberia, was characterized using a polyphasic approach. Our analysis of the 16S rRNA gene sequences showed that 'Aliidiomarina haloalkalitolerans', 'Allidiomarina sanyensis', Idiomarina maris and AIS(T) formed a distinct lineage. The sequence similarities between AIS(T) and the type strains of species of the genera Idiomarina and Aliidiomarina were 91.6-95.1 % and 94.0-96.9 %, respectively. The major isoprenoid quinone of AIS(T) was ubiquinone 8 (Q-8). Predominant cellular fatty acids were iso-C-17:0, iso-C-15:0 and summed feature 9. The genomic DNA G+C content was 45.8 mol%. It is concluded that AIS(T) represents a novel species of the genus Aliidiomarina, and the name Aliidiomarina shirensis sp. nov. is herein proposed for it. The type strain is AIST (=JCM 17761(T)=BCRC 80327(T)). Based on its fatty acid profile and our phylogenetic analysis, we propose that Idiomarina mans be transferred to the genus Aliidiomarina.

WOS
Держатели документа:
[Chiu, Hsiu-Hui
Tang, Sen-Lin] Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan
[Rogozin, Denis Yu.
Degermendzhy, Andrei G.] Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia
[Rogozin, Denis Yu.] Siberian Fed Univ, Krasnoyarsk, Russia
[Huang, Ssu-Po
Shieh, Wung Yang] Natl Taiwan Univ, Inst Oceanog, Taipei 10764, Taiwan
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Chiu, H.H.; Rogozin, D.Y.; Huang, S.P.; Degermendzhy, A.G.; Shieh, W.Y.; Tang, S.L.; National Science Council, Taiwan [NSC 99-2923-B-001-001-MY3]; Russian Foundation for Basic Research [14-04-01060-a]; Siberian Branch of Russian Academy of Sciences, joint Taiwan-Siberian Project [11]

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17.

In vivo study of 2D PHA matrices of different chemical compositions: Tissue reactions and biodegradations [] / T. G. Volova [et al.] // Mater. Sci. Technol. - 2014. - Vol. 30, Is. 5. - P549-557, DOI 10.1179/1743284713Y.0000000470 . - ISSN 1743-2847
Кл.слова (ненормированные):
Biocompatibility -- Biodegradation -- Implantation -- PHA -- Polyhydroxyalkanoates -- Tissue response -- Biocompatibility -- Biodegradable polymers -- Copolymers -- Degradation -- Ion implantation -- Microbiology -- Tissue -- Chemical compositions -- Foreign body giant cells -- Hydroxybutyric acids -- Maximum thickness -- PHA -- Polyhydroxyalkanoates -- Polymer matrices -- Tissue response -- Biodegradation
Аннотация: Matrices based on resorbable polyhydroxyalkanoates (PHAs) of five types {a homopolymer of 3- hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)], 3-hydroxybutyric and 3-hydroxyvaleric acids [P(3HB/3HV)], 3-hydroxybutyric and 3-hydroxyhexanoic acids [P(3HB/3HHx)]} have been constructed and characterised. No significant differences have been found in tissue response to implantation of these PHAs. Non-coarse fibrous capsules that formed around PHA matrices reached their maximum thickness (60-90 ?m) 90 days after implantation; by day 180, the average thickness of the capsules had decreased by 1·5-2·3 times. The number of foreign body giant cells, resorbing PHAs, remained high. In vivo biodegradation behaviour of polymer matrices is related to the chemical composition of the PHA. Matrices prepared from copolymers P(3HB/4HB) and P(3HB/3HHx) exhibited the fastest degradation rates. P3HB/3HV matrices were degraded more slowly, and P3HB matrices were the most durable. In the PHA matrices that were degraded more slowly, giant cell reaction developed later. © 2014 Institute of Materials, Minerals and Mining.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Institute of Modern Biology and Biotechnology, Siberian Federal University, Svobodniy av. 79, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
Engineering Systems Division, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, Cambridge, MA 02139, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Shishatskaya, E.I.; Nikolaeva, E.D.; Sinskey, A.J.

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18.

Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing / O. V. Kolmakova [et al.] // FEMS Microbiol. Ecol. - 2014. - Vol. 89, Is. 2. - P442-450, DOI 10.1111/1574-6941.12355 . - ISSN 1574-6941
Кл.слова (ненормированные):
16S rRNA gene -- Bacterial community -- Diversity -- Yenisei River -- Actinobacteria -- Bacteria (microorganisms) -- Cyanobacteria -- Proteobacteria
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section. © 2014 Federation of European Microbiological Societies.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kolmakova, O.V.; Gladyshev, M.I.; Rozanov, A.S.; Peltek, S.E.; Trusova, M.Y.

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19.

Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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20.

Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms [Text] / A. N. Boyandin [et al.] // Appl. Biochem. Microbiol. - 2012. - Vol. 48, Is. 1. - P28-36, DOI 10.1134/S0003683812010024. - Cited References: 39. - The work was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013) and the Program of Integrated Research of the Presidium of the Siberian Branch of Russian Academy of Sciences (project no. 96). . - 9. - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
POLY-BETA-HYDROXYBUTYRATE
   CHAIN-LENGTH POLYHYDROXYALKANOATES
   DEGRADATION
   FILMS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
   BACTERIA
   ACID
Аннотация: Biodegradation of microbial linear polymers of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) by soil microbial communities of different structures has been studied during two field seasons in different weather conditions. This process was shown to be influenced by the polymer chemical composition, temperature, humidity, and the microbial soil component. The PHA degradation was accompanied by a decrease in the polymer molecular weight and an increase in the degree of crystallinity, indicating the preferential destruction of the amorphous phase compared to the crystalline one. The quantity of the true PHA destructors developing at the surface of the polymer samples was lower than the quantity of accompanying bacteria. The dominant PHA degrading microorganisms under the test conditions were identified as bacteria of the genera Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Xanthomonas and as micromycetes from Penicillium, Paecilomyces, Acremonium, Verticillium, and Zygosporium.

WOS,
SCOPUS,
Полный текст на сайте издателя
Держатели документа:
[Boyandin, A. N.
Volova, T. G.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Prudnikova, S. V.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Filipenko, M. L.
Khrapov, E. A.] Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
[Vasil'ev, A. D.] Russian Acad Sci, Siberian Branch, Kirenskii Inst Phys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A. N.; Prudnikova, S.V.; Filipenko, M.L.; Khrapov, E.A.; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Volova, Tatiana G.; Волова, Татьяна Григорьевна; Government of the Russian Federation [11.G34.31.0013]; Presidium of the Siberian Branch of Russian Academy of Sciences [96]

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