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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trusova M.Y., Kolmakova O.V., Gladyshev M.I.
Заглавие : Seasonal features of consumption of lysine by uncultivated bacterial plankton of Eutrophic water reservoir
Место публикации : Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - С. 391-398. - ISSN 19954255 (ISSN) , DOI 10.1134/S1995425512040154
Ключевые слова (''Своб.индексиров.''): 16s ribosomal rna--biogeochemical function--denaturing gradient gel electrophoresis--eutrophic water reservoir--lysine--microecosystem (mes)--uncultivated bacterial plankton--amino acid--bacterioplankton--bacterium--biogeochemistry--electrokinesis--eutrophication--laboratory method--microbial community--polymerase chain reaction--reservoir--seasonal variation--specialization--bacteria (microorganisms)
Аннотация: Dynamics of bacterial plankton community of eutrophic water reservoir in laboratory microecosystems with amino acid lysine was studied using PCR-DGGE technique. The addition of lysine to the microecosystems resulted in changes in the composition of the bacterial plankton in summer; in particular, a number of Lys1 and Lys2 species (genotypes) that consume this amino acid grew fast in the bacterial community. The plank tonic bacterial communities did not respond to the addition of lysine in spring and late summer. The obtained data confirm the hypothesis for the narrow specialization of bacterial plankton species to the consumption of individual organic substances. В© 2012 Pleiades Publishing, Ltd.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Lobova T.I., Krylova T.Yu., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1571-1579. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00248-4
Ключевые слова (''Своб.индексиров.''): ecosystems--escherichia coli--genes--interferons--luminescence--vaccines--microcosms--microorganisms--fresh water--microorganism--adaptation--article--bacillus subtilis--comparative study--ecosystem--escherichia coli--gene expression--genetics--growth, development and aging--hazardous waste--microbiology--micrococcus--osmotic pressure--plasmid--population dynamics--risk assessment--transgenic organism--adaptation, physiological--bacillus subtilis--containment of biohazards--ecosystem--escherichia coli--fresh water--gene expression--micrococcus--organisms, genetically modified--osmotic pressure--plasmids--population dynamics--risk assessment--water microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Popova L.Y., Lobova T.I., Krylova T.Y., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Колич.характеристики :9 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1571-1579. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00248-4
Примечания : Cited References: 8
Предметные рубрики: GENETICALLY-ENGINEERED MICROORGANISMS
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap(r)Lux(+)) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km(r)lnf(+)), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Y., Kargatova T.V., Maksimova E.E., Belyavskaya V.A.
Заглавие : A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 6. - P637-641. - ISSN 0026-2617
Примечания : Cited References: 13
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Ключевые слова (''Своб.индексиров.''): microecosystem--bacillus subtilis--recombinant plasmid--interferon--adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.
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