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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Degermendzhy A.G., Kosolapova L.G., Vlasik P.V.
Заглавие : Theory, computational method and software for prognosis of migration and distribution of radioactive matters in river ecosystem
Место публикации : COMPUTATIONAL METHODS IN WATER RESOURCES XI, VOL 2: COMPUTATIONAL METHODS IN SURFACE FLOW AND TRANSPORT PROBLEMS: COMPUTATIONAL MECHANICS PUBLICATIONS LTD, 1996. - 11th International Conference on Computational Methods in Water Resources (CMWR 96) (JUL, 1996, CANCUN, MEXICO). - С. 277-284. - 8. - ISBN 1-85312-487-7
Примечания : Cited References: 0
Ключевые слова (''Своб.индексиров.''): mathematical modelling--migration of radionuclides--river ecosystem
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S.
Заглавие : The influence of microbial associations on germination of wheat seeds and growth of seedlings under impact of zinc salts
Место публикации : Advances in Space Research. - 2009. - Vol. 43, Is. 8. - С. 1224-1228. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2008.12.008
Ключевые слова (''Своб.индексиров.''): heavy metals--life support system--microbial associations--microorganisms--wheat seeds--concentration (process)--heavy metals--metals--microorganisms--recycling--salts--seed--zinc--adverse effects--biological recycling--growth and development--heavy metal salts--higher plants--investigate effects--life support system--long-term missions--maximum permissible concentrations--microbial associations--pathogenic organisms--plantlets--recycling systems--root systems--wheat seeds--zinc salts--grain (agricultural product)
Аннотация: The life support systems (LSS) for long-term missions are to use cycling-recycling systems, including biological recycling. Higher plants are the traditional regenerator of air and producer of food. They should be used in many successive generations of their reproduction in LSS. Studies of influence of microbial associations on germination of wheat seeds and on growth of seedlings under impact of heavy metals are necessary because of migration of heavy metals in LSS. Microbial associations are able to stimulate growth of plants, to protect them from pathogenic organisms and from toxicity of heavy metal salts. The goal of this work was to investigate effect of microbial associations on the germination of wheat seeds and on the growth of seedlings under impact of different concentrations of ZnSO4. The results of investigations showed that:(1)Zinc salt had an adverse effect on germination of wheat seeds, beginning with concentrations of 8 MPC (Maximum Permissible Concentration) and higher.(2)Microbial associations (concentrations -104 to 107 cells/ml) were able to decrease (partly or completely) the adverse effect of ZnSO4 on germination of wheat seeds.(3)Concentrations (104-107 cells/ml) of microbial associations were able to decrease partly the adverse effect of zinc salts (intervals: from 1 to 32 MPS{cyrillic}) on the growth and development of wheat plantlets during heterotrophic phase.(4)The root system of plants was more sensitive to the adverse effect of ZnSO4 than shoots of plants. В© 2008 COSPAR.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zuev I. V., Andrushchenko P. Y., Chuprov S. M., Zotina T. A.
Заглавие : Structural Features of Scales of Baikal Grayling Thymallus baicalensis under Conditions of an Altered Hydrological Regime
Место публикации : Inland Water Biol.: Pleiades journals, 2021. - Vol. 14, Is. 1. - С. 60-66. - ISSN 19950829 (ISSN), DOI 10.1134/S1995082920060176
Аннотация: Abstract: The number of circuli between annual scale rings of grayling Thymallus baicalensis Dybowski, 1874 sampled in the Yenisei River in the downstream section of the Krasnoyarsk Hydroelectric Power Station, which does not freeze in winter, have been investigated and compared with populations of grayling from large tributaries of the Yenisei River (Amyl, Kan, Nizhnyaya Tunguska, and Bolshaya Kheta). It has been shown that graylings inhabiting the Yenisei River have a significantly higher (1.5–2.0 times, p 0.01) number of circuli in the second, third, and fourth annual rings of the scales than in the populations from the tributaries, which corresponds to a higher growth rate of the grayling population in the Yenisei River. An assumption is made about the transition of graylings inhabiting the Yenisei River channel in the lower reaches of the hydroelectric station to a sedentary lifestyle. © 2021, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zuev, I., V, Andrushchenko, P. Yu, Chuprov S. M., Zotina T. A.
Заглавие : Structural Features of Scales of Baikal Grayling Thymallus baicalensis under Conditions of an Altered Hydrological Regime
Колич.характеристики :7 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-240003]; Krasnoyarsk Regional Foundation [18-44-240003]; Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academy of SciencesRussian Academy of Sciences
Место публикации : Inland Water Biol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 14, Is. 1. - С. 60-66. - ISSN 1995-0829, DOI 10.1134/S1995082920060176. - ISSN 1995-0837(eISSN)
Примечания : Cited References:39. - This study was partly supported by the Russian Foundation for Basic Research and Krasnoyarsk Regional Foundation for the Support of Scientific and Scientific-Technical Activity (2018-2020), project no. 18-44-240003, and budget themes of the Siberian Federal University and the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences
Аннотация: The number of circuli between annual scale rings of grayling Thymallus baicalensis Dybowski, 1874 sampled in the Yenisei River in the downstream section of the Krasnoyarsk Hydroelectric Power Station, which does not freeze in winter, have been investigated and compared with populations of grayling from large tributaries of the Yenisei River (Amyl, Kan, Nizhnyaya Tunguska, and Bolshaya Kheta). It has been shown that graylings inhabiting the Yenisei River have a significantly higher (1.5-2.0 times, p 0.01) number of circuli in the second, third, and fourth annual rings of the scales than in the populations from the tributaries, which corresponds to a higher growth rate of the grayling population in the Yenisei River. An assumption is made about the transition of graylings inhabiting the Yenisei River channel in the lower reaches of the hydroelectric station to a sedentary lifestyle.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Bolsunovskii A.Ya.
Заглавие : Speciation of artificial radionuclides 60Co, 137Cs, 152Eu, and 241Am in bottom sediments of the Yenisei river
Место публикации : Radiochemistry. - 2008. - Vol. 50, Is. 5. - С. 547-552. - ISSN 10663622 (ISSN) , DOI 10.1134/S1066362208050196
Аннотация: The speciation of artificial radionuclides 60Co, 137Cs, 152Eu, and 241Am in surface layers of bottom sediments of the Yenisei river, collected within the near zone of impact of the Mining and Chemical Combine (Rosatom), was studied. In these samples the radionuclides 60Co, 152Eu, and 241Am are mainly associated with fractions of humic and fulvic acids. The fraction of 152Eu associated with nonsilicate iron is approximately 92%. The migration capability of radionuclides collected near the Atamanovo settlement decreases in the order 152Eu > 241Am > 60Co > 137Cs. For samples collected near Bol'shoi Balchug settlement, this order is as follows: 152Eu ? 241Am > 60Co > 137Cs. The presence of radionuclide-bearing micro-particles in bottom sediments considerably complicates the distribution of radionuclides, in particular, of 241Am and 137Cs, among migration forms. В© 2008 MAIK Nauka.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zuev, Ivan V., Andrushchenko, Pavel Yu., Zotina, Tatiana A.
Заглавие : Seasonal formation of annual rings on the scales of Baikal grayling inhabiting the middle reaches of the Yenisei River under altered temperature regime
Колич.характеристики :10 с
Коллективы : Russian Foundation for Basic Research, Government of the Krasnoyarsk Territory; Krasnoyarsk Regional Scientific Foundation [20-44-240009]
Место публикации : Environ. Biol. Fishes: SPRINGER, 2021. - Article in press. - ISSN 0378-1909, DOI 10.1007/s10641-021-01155-y. - ISSN 1573-5133(eISSN)
Примечания : Cited References:40. - The research was supported by the grant of Russian Foundation for Basic Research, Government of the Krasnoyarsk Territory, and Krasnoyarsk Regional Scientific Foundation No. 20-44-240009.
Предметные рубрики: THYMALLUS-ARCTICUS
FLOW REGULATION
SOCKEYE-SALMON
SOMATIC GROWTH
Аннотация: The seasonal formation of annual rings on the scales of Baikal grayling from the middle reaches of the Yenisei River has been studied to find out the reasons for the high growth rate of the grayling under altered temperature regime downstream of the dam of the Krasnoyarsk Hydroelectric Plant. The number of circuli outside the last identified annuli and in the second, third, and fourth completed annuli was estimated on 569 fish caught during the annual cycle. The von Bertalanffy growth function was used to describe the increment in the number of circuli over a year. The calculation showed that a new annual ring was produced in July. In November, there was no statistically significant difference between the circulus number in scale increment and the circulus number in the corresponding completed annuli of older fish. Thus, despite the increased duration of the period with optimal water temperatures downstream of the dam of the Krasnoyarsk Hydroelectric Plant, fish growth was observed in a limited period of the year, from July to November. The probable reason for the high growth rate of grayling in the study area is that the fish do not have to waste energy on seasonal migration to the tributaries. Taking into account that grayling biomass production in the middle Yenisei occurs from July to November, we can recommend shifting the dates of commercial fishing for grayling to the end of this period-November-December.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zuev I. V., Andrushchenko P. Y., Zotina T. A.
Заглавие : Seasonal formation of annual rings on the scales of Baikal grayling inhabiting the middle reaches of the Yenisei River under altered temperature regime
Место публикации : Environ. Biol. Fishes: Springer Science and Business Media B.V., 2021. - Article in press. - ISSN 03781909 (ISSN), DOI 10.1007/s10641-021-01155-y
Аннотация: The seasonal formation of annual rings on the scales of Baikal grayling from the middle reaches of the Yenisei River has been studied to find out the reasons for the high growth rate of the grayling under altered temperature regime downstream of the dam of the Krasnoyarsk Hydroelectric Plant. The number of circuli outside the last identified annuli and in the second, third, and fourth completed annuli was estimated on 569 fish caught during the annual cycle. The von Bertalanffy growth function was used to describe the increment in the number of circuli over a year. The calculation showed that a new annual ring was produced in July. In November, there was no statistically significant difference between the circulus number in scale increment and the circulus number in the corresponding completed annuli of older fish. Thus, despite the increased duration of the period with optimal water temperatures downstream of the dam of the Krasnoyarsk Hydroelectric Plant, fish growth was observed in a limited period of the year, from July to November. The probable reason for the high growth rate of grayling in the study area is that the fish do not have to waste energy on seasonal migration to the tributaries. Taking into account that grayling biomass production in the middle Yenisei occurs from July to November, we can recommend shifting the dates of commercial fishing for grayling to the end of this period—November–December. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : Prey-predator model with purposeful migration of individuals
Колич.характеристики :7 с
Место публикации : Zhurnal Obshchei Biol.: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 62, Is. 3. - P239-245. - ISSN 0044-4596
Примечания : Cited References: 22
Аннотация: Two species "prey-predator model" where the individuals purposefully migrate between two habitats is considered. A migration is regarded as transference from one habitat to another yielding in maximization of average (over two habitats) coefficient of reproduction. Targeted migration results in the increase in average abundance of population comparing with discrete analogies of classic equations of Volterra and also to the enlargement of the area of the admissible meanings of parameters. A great variety of dynamic regimes has been observed, some of them could be interpreted as outbreak explosion.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Lobova T.I., Krylova T.Yu., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1571-1579. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00248-4
Ключевые слова (''Своб.индексиров.''): ecosystems--escherichia coli--genes--interferons--luminescence--vaccines--microcosms--microorganisms--fresh water--microorganism--adaptation--article--bacillus subtilis--comparative study--ecosystem--escherichia coli--gene expression--genetics--growth, development and aging--hazardous waste--microbiology--micrococcus--osmotic pressure--plasmid--population dynamics--risk assessment--transgenic organism--adaptation, physiological--bacillus subtilis--containment of biohazards--ecosystem--escherichia coli--fresh water--gene expression--micrococcus--organisms, genetically modified--osmotic pressure--plasmids--population dynamics--risk assessment--water microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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10.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Popova L.Y., Lobova T.I., Krylova T.Y., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Колич.характеристики :9 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1571-1579. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00248-4
Примечания : Cited References: 8
Предметные рубрики: GENETICALLY-ENGINEERED MICROORGANISMS
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap(r)Lux(+)) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km(r)lnf(+)), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Motolygin S.A., Sadovsky M.G., Chukov D.A.
Заглавие : Model of population with optimally migrating individuals
Колич.характеристики :10 с
Место публикации : Zhurnal Obshchei Biol.: MEZHDUNARODNAYA KNIGA, 1999. - Vol. 60, Is. 4. - P450-459. - ISSN 0044-4596
Примечания : Cited References: 21
Аннотация: The new model was proposed to study the dynamic of spatially distributed populations with nonrandom migrating individuals. All migrations are resulted in maximization of the average net reproduction rate taking into account the cost of migration. The model belongs to a box type: individuals migrate between two stations and have complete information about living conditions both in the stations of residence and migration. The information is determined by dependence of reproduction rate on the abundance of individuals in the station. Numerical experiments showed that taking into account the goal-directed migrations caused the following effects: significant extension of the value of external conditions leading to the stable existence of population; increase in the average abundance of population; broadening of the observed set of dynamic regimes. The limitation of the model application are discussed.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Bolsunovskii A.Ya., Sukhorukov F.V., Kazbanov V.I., Makarova I.V., Legler E.V.
Заглавие : Model assessment of the migration capability of transuranium radionuclides (241Am and Pu isotopes) and 152Eu in the system bottom sediments-Yenisei river water by chemical fractionation technique
Место публикации : Radiochemistry. - 2005. - Vol. 47, Is. 4. - С. 415-421. - ISSN 10663622 (ISSN) , DOI 10.1007/s11137-005-0112-8
Аннотация: Sorption of radiotracers 241Am and 242Pu and weighable amounts of uranium and stable Eu on bottom sediments in the simulated system bottom sediments-Yenisei river water was studied. At a contact time of 40 min these metals are completely sorbed on the bottom sediments, presumably via association with organomineral complexes and hydrated gels. Along with sorption, these metals can form soluble complexes and stable colloids (pseudocolloids). The distribution factors of 241Am and 242Pu, and stable Eu in the simulated system between bottom sediments and liquid phases were calculated from the results of sorption experiments. The introduced 241Am and 152Eu initially present in radioactively contaminated bottom sediments of the Yenisei river show similar pattern of distribution over differently mobile fractions. The distribution of weighable amounts of stable Eu significantly differs from that of initially present 152Eu and tracer 241Am due to decelerated sorption of stable Eu and its considerably higher concentration. A significant part of 242Pu, 241Am, and 152Eu is associated with mobile fractions of bottom sediments, which, under certain environmental conditions, can pass into river water as migrating species. В©2005 Pleiades Publishing, Inc.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Gribovskaya I.V., Ivanova E.A., Moskvichova A.V., Muchkina E.Ya., Chuprov S.M.
Заглавие : Metal Concentrations in the Ecosystem and Around Recreational and Fish-Breeding Pond Bugach
Место публикации : Water Resources. - 2001. - Vol. 28, Is. 3. - С. 288-296. - ISSN 00978078 (ISSN) , DOI 10.1023/A:1010400807660
Аннотация: Data of two field studies were used to analyze the concentrations of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb in the water, bottom deposits, zoobenthos, fish, and macrophytes of the Pond Bugach and in the soils near the pond. It was established that the majority of metals in the soils and bottom deposits correlate and their concentrations are governed by the universal geochemical factors of the region. The heavy metals were recognized that originate from anthropogenic sources and their concentrations were found to exceed the maximum admissible values for different components of the ecosystem. Five types of heavy metals migration were recognized in the soil-bottom deposits-zoobenthos-fish chain and in macrophytes. Statistically significant difference was found to exist between the concentrations of some heavy metals in the muscles of fish species with different food types (crucian carp and perch) as well as between the correlations of metals. The recorded concentrations were compared with the concentrations of metals measured in the last decade in other limnetic ecosystems in Siberia, Europe, North America, and China.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina T.A., Tolomeyev A.P., Degermendzhy N.N.
Заглавие : Lake Shira, a Siberian salt lake: Ecosystem structure and function. 1. Major physico-chemical and biological features
Место публикации : International Journal of Salt Lake Research. - 1999. - Vol. 8, Is. 3. - С. 211-232. - ISSN 10370544 (ISSN) , DOI 10.1023/A:1009030120324
Ключевые слова (''Своб.индексиров.''): bacterioplankton--blue-green algae--meromictic salt lake--phytoplankton--vertical structure--zooplankton--community composition--ecosystem function--ecosystem structure--migration--relative abundance--saline lake--vertical distribution--russian federation--arctodiaptomus salinus--brachionus plicatilis--cyclotella tuberculata--dictyosphaerium tetrachotomum--hexarthra oxiuris--lyngbya contorta--microcystis ichtyoblabe
Аннотация: The specific composition, vertical distribution and daily migration of plankton in a meromictic salt lake, Lake Shira, have been investigated. The main structural components of the ecosystem are bacteria, algae and crustaceans. Since the late 1970s, blue-green algae are reported as dominant. Nowadays the phytoplankton is dominated by Lyngbya contorta, Microcystis ichtyoblabe (Cyanophyta), Cyclotella tuberculata (Bacillariophyta) and Dictyosphaerium tetrachotomum (Chlorophyta). The zooplankton of Lake Shira comprises Arctodiaptomus salinus (Copepoda), Brachionus plicatilis and Hexarthra oxiuris (Rotatoria). Investigation revealed a stratified vertical distribution of all dominant species except Cyclotella with a maximum at a depth of 12-15 m during the summer growth period. Zooplanktons differ in the pattern of distribution and daily migration, but most zooplankton tend to concentrate near the surface. The possible reasons for the vertical distribution of the plankton are discussed.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubovtseva, I. Yu, Aksenenko M. B., Nikolaeva E. D., Averchuk A. S., Moshev, A., V, Savchenko A. A., Markova, S., V, Ruksha T. G.
Заглавие : FOXC1-Mediated Effects of miR-204-5p on Melanoma Cell Proliferation
Колич.характеристики :8 с
Место публикации : Mol. Biol.: PLEIADES PUBLISHING INC, 2021. - Vol. 55, Is. 4. - С. 610-617. - ISSN 0026-8933, DOI 10.1134/S0026893321020199. - ISSN 1608-3245(eISSN)
Примечания : Cited References:24
Предметные рубрики: FOXC1
Аннотация: MicroRNAs epigenetically regulate physiological and pathological processes. Previously, we found that miR-204-5p is expressed at low levels in melanoma cells, and an increase in its level leads to a change in proliferation, migration, and invasion of these cancer cells. Now, using bioinformatics analysis, it has been shown that the target of miR-204-5p is FOXC1 transcription factor, which is implicated in carcinogenesis. Using the luciferase reporter assay, it was found that miR-204-5p suppresses expression of the FOXC1 gene by binding to its 3' non-coding region. Transfection of small interfering RNA (siRNA) targeting FOXC1 into melanoma cells caused a decrease in miR-204-5p levels, which is consistent with the generally accepted concept of feedback regulation of miRNA expression by target genes. According to the results of the MTT test and fluorescence microscopy, the proliferation level of melanoma cells under the influence of siRNA to FOXC1 decreased 72 h after transfection. Changes in the ratio of cells by cell cycle phase were analyzed using flow cytometry. Regulatory relationships between FOXC1 and miR-204-5p, and an inhibitory effect of FOXC1 knockdown on melanoma cell proliferation were revealed. Based on the results, it can be assumed that miR-204-5p regulates proliferation of melanoma cells by affecting FOXC1 expression.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubovtseva I. Y., Aksenenko M. B., Nikolaeva E. D., Averchuk A. S., Moshev A. V., Savchenko A. A., Markova S. V., Ruksha T. G.
Заглавие : FOXC1-Mediated Effects of miR-204-5p on Melanoma Cell Proliferation
Место публикации : Mol Biol (Mosk): NLM (Medline), 2021. - Vol. 55, Is. 4. - С. 667-675. - ISSN 00268984 (ISSN), DOI 10.31857/S0026898421030058
Аннотация: MicroRNAs epigenetically regulate physiological and pathological processes. Previously, we found that miR-204-5p is expressed at low levels in melanoma cells, and an increase in its level leads to a change in proliferation, migration, and invasion of these cancer cells. Now, using bioinformatics analysis, it has been shown that the target of miR-204-5p is FOXC1 transcription factor, which is implicated in carcinogenesis. Using the luciferase reporter assay, it was found that miR-204-5p suppresses expression of the FOXC1 gene by binding to its 3' non-coding region. Transfection of small interfering RNA (siRNA) targeting FOXC1 into melanoma cells caused a decrease in miR-204-5p levels, which is consistent with the generally accepted concept of feedback regulation of miRNA expression by target genes. According to the results of the MTT test and fluorescence microscopy, the proliferation level of melanoma cells under the influence of siRNA to FOXC1 decreased 72 h after transfection. Changes in the ratio of cells by cell cycle phase were analyzed using flow cytometry. Regulatory relationships between FOXC1 and miR-204-5p, and an inhibitory effect of FOXC1 knockdown on melanoma cell proliferation were revealed. Based on the results, it can be assumed that miR-204-5p regulates proliferation of melanoma cells by affecting FOXC1 expression.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A.Ya., Ermakov A.I., Sobolev A.I., Degermendzhi A.G.
Заглавие : First evidence for concentrations of transuranium curium in the Yenisei floodplain ecosystem
Место публикации : Doklady Earth Sciences. - 2006. - Vol. 409, Is. 5. - С. 810-813. - ISSN 1028334X (ISSN) , DOI 10.1134/S1028334X06050308
Ключевые слова (''Своб.индексиров.''): actinide--background level--floodplain--fluvial deposit--radionuclide migration--river pollution--soil pollution--eurasia--krasnoyarsk [russian federation]--russian federation--yenisei river--zheleznogorsk
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E. I., Nikolaeva E. D., Vinogradova O. N., Volova T. G.
Заглавие : Experimental wound dressings of degradable PHA for skin defect repair
Место публикации : J. Mater. Sci. Mater. Med.: Springer New York LLC, 2016. - Vol. 27, Is. 11. - ISSN 09574530 (ISSN) , DOI 10.1007/s10856-016-5776-4
Ключевые слова (''Своб.индексиров.''): biomaterials--cell culture--cells--cytology--enzyme inhibition--fibroblasts--membranes--natural polymers--nonwoven fabrics--polymer films--proteins--stem cells--weaving--electrospun membranes--extracellular matrix protein--fibroblast cells--hydroxyderivative--mesenchymal stem cell--non-woven membranes--wound dressing materials--wound healing process--tissue
Аннотация: The present study reports construction of wound dressing materials from degradable natural polymers such as hydroxy derivatives of carboxylic acids (PHAs) and 3-hydroxybutyrate/4-hydroxybutyrate [P(3HB/4HB)] as copolymer. The developed polymer films and electrospun membranes were evaluated for its wound healing properties with Grafts—elastic nonwoven membranes carrying fibroblast cells derived from adipose tissue multipotent mesenchymal stem cells. The efficacy of nonwoven membranes of P(3HB/4HB) carrying the culture of allogenic fibroblasts was assessed against model skin defects in Wistar rats. The morphological, histological and molecular studies revealed the presence of fibroblasts on dressing materials which facilitated wound healing, vascularization and regeneration. Further it was also observed that cells secreted extracellular matrix proteins which formed a layer on the surface of membranes and promoted the migration of epidermal cells from the neighboring tissues surrounding the wound. The wounds under the P(3HB/4HB) membrane carrying cells healed 1.4 times faster than the wounds under the cell-free membrane and 3.5 times faster than the wounds healing under the eschar (control).The complete wound healing process was achieved at Day 14. Thus the study highlights the importance of nonwoven membranes developed from degradable P(3HB/4HB) polymers in reducing inflammation, enhancing angiogenic properties of skin and facilitating better wound healing process. © 2016, Springer Science+Business Media New York.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : U - Popova LYu, Pechurkin N.S., Maksimova E.E., Kargatova T.V. U - Krylova TYu, Lobova T.I., Boyandin A.N.
Заглавие : Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 193-197. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial dna--recombinant dna--adaptation--article--ecosystem--escherichia coli--genetics--microbiology--plasmid--risk assessment--adaptation, biological--dna, bacterial--dna, recombinant--ecosystem--escherichia coli--microbiology--plasmids--risk assessment--soil microbiology--water microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Kalyakina O.P., Bolsunovskii A.Y.
Заглавие : Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and gamma-ray spectrometry
Место публикации : J. Anal. Chem.: MAIK NAUKA/INTERPERIODICA, 2006. - Vol. 61, Is. 4. - С. 354-358. - 5. - ISSN 1061-9348, DOI 10.1134/S1061934806040101
Примечания : Cited References: 11
Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and gamma-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO42- and Cl- anions by a solid phase with the release of NO3-, PO43-, and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of gamma-ray spectrometry, it was found that the release of Co-60, Eu-152, and Am-241 radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: similar to 25% Am-241, similar to 3% Eu-152, and similar to 0.8% Co-60 in the system with added humic acid or 0.8% Eu-152 and 0.1% Co-60 in the system without the addition of humic acid. The Am-241 radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of Co-60, Eu-152, and Am-241 anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of K-40 and Cs-137 radionuclides.
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