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Electrophoresis of nanodiamond powder for cold cathode fabrication [Text] / A. N. Alimova [et al.] // J. Vac. Sci. Technol. B. - 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - P715-718, DOI 10.1116/1.590625. - Cited References: 5 . - 4. - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
FIELD-EMISSION
TIPS
Аннотация: The process of electrophoresis/dielectrophoresis of shock-synthesized nanodiamond powders on the large-area Si field emission arrays was studied. The process was found to be very sensitive to the modification of nanodiamond powder. The growth rate and film morphology of nanodiamond coatings on Si tips were investigated. Results in fabrication and testing of nanodiamond-based cold cathodes and sealed device prototypes are presented. (C) 1999 American Vacuum Society. [S0734-211X(99)06002-3].

Держатели документа:
Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia
ISTOK, State Res & Prod Corp, Fryazino 141120, Moscow Region, Russia
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
Russian Fed Nucl Ctr, Snezhinsk 456770, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Alimova, A.N.; Chubun, N.N.; Belobrov, P.I.; Detkov, P.Y.; Zhirnov, V.V.

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Physicochemical properties of Al2O3 powder produced by explosive synthesis [Text] / A. A. Bukaemskii, A. G. Beloshapko, A. P. Puzyr // Combust. Explos. - 2000. - Vol. 36, Is. 5. - P. 660-666, DOI 10.1007/BF02699530. - Cited References: 18 . - ISSN 0010-5082

РУБ Thermodynamics + Energy & Fuels + Engineering, Multidisciplinary + Engineering, Chemical + Materials Science, Multidisciplinary

Аннотация: The phase, dispersive, and morphological features of an aluminum oxide powder produced by explosive synthesis are examined experimentally. It is shown that the particle-size distribution has three distinct maxima, which are due to different combustion regimes. The relationship between the sizes and morphology of the starting powder and the product is determined. Considerable attention is given to a study of the ultrafine fraction of the product powder. The ultrafine particles are shown to have a regular spherical shape, and sinters are not revealed. Besides spherical particles, the synthesized powder contains faceted crystals. X-ray phase analysis of the ultrafine fraction of the synthesized powders shows that this fraction consists only of the metastable phases of the oxide - the delta- or oxynitride modifications. The delta -modification differs from that described in the literature.

WOS
Держатели документа:
Krasnoyarsk State Univ, Inst Physicotech, Krasnoyarsk 660036, Russia
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bukaemskii, A.A.; Beloshapko, A.G.; Puzyr, A.P.

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Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems [Text] / E. E. Maksimova, L. Y. Popova ; ed. a, EE Maksim // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1581-1586, DOI 10.1016/S0273-1177(01)00249-6. - Cited References: 17 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Y.; Maksim, a, EE \ed.\

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Dobutamine prevents experimental postintoxication liver cirrhosis in mice / A. P. Vinokurov, A. V. Eremeev, N. A. Setkov // Bulletin of Experimental Biology and Medicine. - 2002. - Vol. 134, Is. 1. - P. 43-46, DOI 10.1023/A:1020604621142 . - ISSN 0007-4888
Кл.слова (ненормированные):
Dobutamine -- Hepatocytes -- Liver cirrhosis -- Proliferation -- adrenergic receptor stimulating agent -- alpha 1 adrenergic receptor stimulating agent -- beta 1 adrenergic receptor stimulating agent -- beta 2 adrenergic receptor stimulating agent -- carbon tetrachloride -- catecholamine derivative -- dobutamine -- epidermal growth factor -- animal cell -- animal experiment -- animal model -- animal tissue -- article -- cell activity -- cell culture -- cell death -- cell proliferation -- cell stimulation -- chronic hepatitis -- connective tissue -- controlled study -- disease association -- drug effect -- drug potency -- experimental test -- female -- liver cell -- liver cirrhosis -- liver fibrosis -- liver hyperplasia -- liver necrosis -- liver parenchyma -- liver regeneration -- liver toxicity -- morphology -- mouse -- nonhuman -- toxicity testing -- Adrenergic beta-Agonists -- Animals -- Carbon Tetrachloride -- Cell Division -- Dobutamine -- Dose-Response Relationship, Drug -- Female -- Hepatocytes -- Liver -- Liver Cirrhosis -- Mice -- Mice, Inbred BALB C -- Animalia
Аннотация: Various chronic inflammatory and necrotic processes in the liver parenchyma are accompanied by pathological morphofunctional changes, which are associated with hepatocyte death and hyperplasia of the connective tissue. Regeneration of the liver parenchyma should include not only prevention of fibrosis, but also stimulation of hepatocyte prolifearation. The adrenoceptor agonist dobutamine stimulated proliferative activity of cultured hepatocytes and prevented the development of postintoxication liver cirrhosis in mice produced by chronic poisoning with CCl4.

Scopus
Держатели документа:
Department of General Surgery, Krasnoyarsk State Medical Academy, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vinokurov, A.P.; Eremeev, A.V.; Setkov, N.A.

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Study of the biological properties of polyoxyalkanoates in the in vivo chronic experiment / E. I. Shishatskaya, T. G. Volova, T. G. Popova // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 29-33 . - ISSN 0025-8075
Кл.слова (ненормированные):
biomaterial -- polymer -- animal -- article -- bioremediation -- comparative study -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Biocompatibility -- Immunology -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Rat models -- Suture threads -- Biomaterials
Аннотация: Chronic experiments on mature Wistar rats studied the biological properties of suture threads from two types of polyoxyalkanoates (POA), such as polyoxybutyrate (POB) and the copolymer of POB and polyoxyvalerate (POV) (POB/POV) as compared to the conventional surgical material (silk and catgut). The physiological and biochemical parameters that reflect the status of the animals, including their behavior, height and development, the weight of the body and viscera, the morphology of peripheral blood, the biochemical parameters of blood, the activity of some enzymes, as well as histological findings of the viscera, and lymphoid tissue responses were assessed. POA was found to produce no negative effect on the physiological, biochemical, and functional characteristics of the animals irrespective of the duration of contact with the internal medium of the body and of the chemical composition of a material.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Popova, T.G.

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Studies of Hydrochemical and Kinetic Characteristics of Small Water Bodies in the Context of Their Eutrophication / I. V. Gribovskaya [et al.] // Water Resources. - 2003. - Vol. 30, Is. 1. - P68-71, DOI 10.1023/A:1022055802258 . - ISSN 0097-8078
Кл.слова (ненормированные):
eutrophication -- hydrodynamics -- phytoplankton -- pond -- water quality -- Eurasia -- Krasnoyarsk [Russian Federation] -- Russian Federation
Аннотация: Comparison study data on the hydrochemical parameters, bacterio- and phytoplankton, and reduction-oxidation characteristics of two ponds in Krasnoyarsk are presented. These water bodies are of interest due to the fact that the ecosystems of these natural model objects respond to eutrophication in different ways. It is assumed that the reason for this difference is in the hydrophysical characteristics depending on the morphology of the basins of the water bodies rather than in the hydrochemical characteristics.

Scopus
Держатели документа:
Siberian Branch, Russian Academy of Sciences, Academgorodok, Krasnoyarsk 660036, Russian Federation
Krasnoyarsk Stt. Agrarian University, prosp. Mira 88, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gribovskaya, I.V.; Ivanova, E.A.; Kalacheva, G.S.; Kravchuk, E.S.

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A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - 9. - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P. 915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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10.

Heterogeneity of the populations of marine luminescent bacteria Photobacterium leiognathi under different conditions of cultivation / S. E. Medvedeva, O. A. Mogil'naya, L. Yu. Popova // Microbiology. - 2006. - Vol. 75, Is. 3. - P292-299, DOI 10.1134/S002626170603009X . - ISSN 0026-2617
Кл.слова (ненормированные):
Bioluminescence -- Luminescent bacteria -- Morphology of colonies -- Bacteria (microorganisms) -- Photobacterium leiognathi
Аннотация: Manifestation of pleiotropic effects in the isogenic variants of the luminescent bacterium Photobacterium leiognathi 54 was investigated. The decrease or increase of the expression level of bioluminescence was caused by changes in lux operon regulation. The dynamics of the bioluminescence of dark and dim variants did not differ from the dynamics of the initial luminescent variant, but dependence of the level of luminescence intensity on the exogenous autoinducer of the lux operon was revealed. The investigated variants of P. leiognathi 54 inherited fairly stable morphological characteristics, colony architectonics, level of luminescence, and activity of some enzymes; variants with reduced bioluminescence formed colonies of the S type. Stable bright variants with S-and R-type colonies appeared both in the initial strain population and in the dark variant population, but with smaller frequency. Populations of the bright variant with R-type colonies were most heterogeneous; this can be determined by the lack of glucose repression of the bioluminescence in contrast to other investigated inherited variants of P. leiognathi. В© Pleiades Publishing, Inc., 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, 50, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Mogil'naya, O.A.; Popova, L.Yu.

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11.

Anoxygenic phototrophic bacterial community of Lake Shira (Khakassia) / O. N. Lunina [et al.] // Microbiology. - 2007. - Vol. 76, Is. 4. - P469-479, DOI 10.1134/S0026261707040133 . - ISSN 0026-2617
Кл.слова (ненормированные):
Anoxygenic photosynthesis -- Anoxygenic phototrophic bacteria -- Meromictic brackish lakes -- Ahrensia kielensis -- Bacteria (microorganisms) -- Chlorobi -- Chlorobium -- Chlorobium limicola -- Chromatiaceae -- Lamprocystis purpurea -- Photobacteria -- Proteobacteria -- Rhodomicrobium -- Rhodovulum -- Rhodovulum strictum -- Thiocapsa roseopersicina
Аннотация: The anoxygenic phototrophic bacterial community of the brackish meromictic Lake Shira (Khakassia) was investigated in August 2001, July 2002, and February-March 2003. In all the periods of investigation, the prevailing microorganisms were purple sulfur bacteria similar to Lamprocystis purpurea in morphology and pigment composition. Their highest number (3 ? 10 5 cells/ml) was recorded in July 2002 at the depth of 15 m. According to 16S rRNA gene analysis, the strain of purple sulfur bacteria isolated in 2001 and designated ShAm01 exhibited 98.6% similarity to the type strain of Thiocapsa roseopersicina and 97.1-94.4% similarity to the type strains of Tca. pendens, Tca. litoralis, and Tca. rosea. The minor microorganisms of the anoxygenic phototrophic bacterial community within the period of investigation were nonsulfur purple bacteria phylogenetically close to Rhodovulum strictum (98.3% similarity, strain ShRb01), Ahrensia kielensis (of 93.9% similarity, strain ShRb02), Rhodomicrobium vannieli (of 99.7% similarity, strain ShRmc01), and green sulfur bacteria, phylogenetically close to Chlorobium limicola (of 98.7% similarity, strain ShCl03). В© 2007 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russian Federation
Skryabin Institute of Microbial Biochemistry and Physiology, Russian Academy of Sciences, Pushchino
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lunina, O.N.; Bryantseva, I.A.; Akimov, V.N.; Rusanov, I.I.; Barinova, E.S.; Lysenko, A.M.; Rogozin, D.Yu.; Pimenov, N.V.

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12.

Infochemical-mediated trophic interactions between the rotifer Brachionus calyciflorus and its food algae / A. M. Verschoor, Y. S. Zadereev, W. M. Mooij // Limnology and Oceanography. - 2007. - Vol. 52, Is. 5. - P2109-2119 . - ISSN 0024-3590
Кл.слова (ненормированные):
alga -- aquatic ecosystem -- assimilation efficiency -- experimental study -- feeding behavior -- food web -- freshwater environment -- ingestion rate -- trophic interaction -- algae -- Brachionus calyciflorus -- Rotifera -- Scenedesmus -- Scenedesmus obliquus
Аннотация: We studied how chemicals obtained as filtrates from algal monocultures (algal chemicals) and from rotifer cultures with or without algae (rotifer chemicals) affected feeding rates of the rotifer Brachionus calyciflorus on its food algae, both directly and indirectly (through chemical-induced changes in algal morphology). Algal chemicals had a strong stimulating effect on the feeding rate of B. calyciflorus, but these effects were counteracted by rotifer chemicals. In functional response experiments, rotifer chemicals lowered maximum ingestion rates and had strong effects on assimilation rates and assimilation efficiencies of B. calyciflorus, probably due to the release of unspecific (auto)toxic metabolites. Furthermore, rotifer chemicals induced colony formation in the food alga Scenedesmus obliquus. Above the optimum particle size for ingestion by B. calyciflorus, larger algal colony sizes increased the food-handling time, thus lowering ingestion and assimilation rates. Through their effects on trophic interactions, infochemicals may play a role in structuring and the functioning of aquatic food webs. В© 2007, by the American Society of Limnology and Oceanography, Inc.

Scopus
Держатели документа:
Netherlands Institute of Ecology (NIOO-KNAW), Centre for Limnology, Rijksstraatweg 6, 3631 AC Nieuwersluis, Netherlands
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Verschoor, A.M.; Zadereev, Y.S.; Mooij, W.M.

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13.

Microdistribution of 241Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River / L. Bondareva [et al.] // Journal of Environmental Radioactivity. - 2010. - Vol. 101, Is. 1. - P16-21, DOI 10.1016/j.jenvrad.2009.08.003 . - ISSN 0265-931X
Кл.слова (ненормированные):
Alpha-track analysis -- Americium -- Elodea canadensis -- Liquid-scintillation spectrometry -- Microdistribution -- Alpha-track analysis -- Elodea canadensis -- Microdistribution -- Scintillation spectrometry -- Track analysis -- Liquids -- Luminescence -- Radioisotopes -- Scintillation -- Spectrometry -- Spectroscopy -- Americium -- americium 241 -- americium -- americium -- bioaccumulation -- experimental study -- leaf -- macrophyte -- mass spectrometry -- morphology -- radionuclide -- spatial distribution -- stem -- submerged vegetation -- tracking -- aquatic flora -- article -- bioaccumulation -- concentration (parameters) -- elodea canadensis -- environmental radioactivity -- isotope analysis -- isotope tracing -- leaf lamina -- leaf surface -- macrophyte -- nonhuman -- plant cell -- plant morphology -- plant stem -- radioisotope distribution -- river -- chemistry -- Hydrocharitaceae -- metabolism -- plant -- plant leaf -- radiation monitoring -- river -- water pollutant -- Eurasia -- Russian Federation -- Yenisei River -- Elodea canadensis -- Americium -- Hydrocharitaceae -- Plant Leaves -- Plant Shoots -- Plant Stems -- Radiation Monitoring -- Rivers -- Water Pollutants, Radioactive
Аннотация: A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide 241Am among different components of the plant. The total amount of 241Am added to the experimental system was 1850 В± 31 Bq/L. The total amount of 241Am accumulated by the plants was 182 Bq per sample, or 758,333 В± 385 Bq/kg dry mass. It has been found that the major portion of 241Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of 241Am within different components of the submerged plant E. canadensis was not uniform. 241Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem. В© 2009 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.; Vlasova, I.; Mogilnaya, O.; Bolsunovsky, A.; Kalmykov, S.

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14.

Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals / E. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - P2185-2203, DOI 10.1163/092050610X537138 . - ISSN 0920-5063
Кл.слова (ненормированные):
14C products of polymer resorption -- intravenous administration -- microparticles -- Resorbable polyhydroxybutyrate -- tissues of internal organs -- 3-hydroxybutyric acid -- sup14/supC products of polymer resorption -- Adverse effect -- Drug-delivery systems -- Growth and development -- High molecular weight -- Initial molecular weight -- Initial values -- Internal organs -- Intravenous administration -- Laboratory animals -- Local response -- matrix -- Mean diameter -- Micro-particles -- Polymer degradation -- Polymer microparticles -- Preparation process -- Radioactivity level -- Residual content -- Resorbable -- Sustained-release -- Tail veins -- Wistar rat -- Animals -- Biocompatibility -- Degradation -- Histology -- Molecular weight -- Morphology -- Radiation -- Radioactivity -- Rats -- Tissue -- poly(3 hydroxybutyric acid) -- animal experiment -- animal tissue -- article -- biocompatibility -- concentration process -- controlled study -- degradation -- drug delivery system -- female -- gel permeation chromatography -- heart -- kidney parenchyma -- liver -- lung parenchyma -- molecular weight -- nonhuman -- organ culture -- priority journal -- radioactivity -- rat -- spleen -- Animals -- Animals, Laboratory -- Biocompatible Materials -- Drug Delivery Systems -- Female -- Infusions, Intravenous -- Materials Testing -- Microspheres -- Molecular Weight -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Animalia -- Rattus -- Rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.

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Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.; Goreva, A.; Kalacheva, G.; Volova, T.

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15.

Ultrastructure and morphology of the bioluminescent system in "bioluminescent" and "non-bioluminescent" scale-worms (Polychaeta, Polynoidae) [Text] / N. B. Aneli [et al.] // Luminescence. - 2014. - Vol. 29. - P41-42. - Cited References: 5 . - ISSN 1522-7235. - ISSN 1522-7243
Рубрики:
ORGANS

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Держатели документа:
[Aneli, N. B.] Russian Acad Sci EZAN, Expt Factory Sci Engn, Chernogolovka, Moscow Region, Russia
[Belousova, A. A.] Moscow MV Lomonosov State Univ, Fac Biol, Dept Invertebrate Zool, Moscow, Russia
[Saprunova, V. B.] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow, Russia
[Petushkov, V. N.] Russian Acad Sci, Siberian Branch, Lab Photobiol, Inst Biophys, Krasnoyarsk, Russia
[Kondrashov, F. A.
Plyuscheva, M. V.] CRG, Barcelona, Spain
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Aneli, N.B.; Belousova, A.A.; Saprunova, V.B.; Petushkov, V.N.; Kondrashov, F.A.; Plyuscheva, M.V.

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16.

A narrowing of the phenotypic diversity range after large rearrangements of the karyotype in salmonidae: The relationship between saltational genome rearrangements and gradual adaptive evolution / A. A. Makhrov // Genes. - 2017. - Vol. 8, Is. 11, DOI 10.3390/genes8110297 . - ISSN 2073-4425
Кл.слова (ненормированные):
Ecology -- Evolution -- Genome -- Karyotype -- Morphology -- ecology -- gene rearrangement -- genome -- karyotype -- morphology -- nonhuman -- salmonid
Аннотация: The problem of how a gradual development of ecological and morphological adaptations combines with large genome rearrangements, which have been found to occur in the phylogeny of many groups of organisms, is a matter of discussion in the literature. The objective of this work was to study the problem with the example of salmonids, whose evolution included at least six events of multiple chromosome fusions. Large karyotype rearrangements are associated with a decrease in ecological and morphological diversity in salmonids. In the above example, genome rearrangements seem to distort the function of the genetic systems that are responsible for the occurrence of certain ecological forms in salmonids. © 2017 by the authors; Licensee MDPI, Basel, Switzerland.

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Держатели документа:
A.N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, Moscow, Russian Federation
Institute of Biophysics of Siberian Branch of Federal Research Center, “Krasnoyarsk Science Center” of Russian Academy of Sciences, Akademgorodok, 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Makhrov, A. A.

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17.

Green light to an integrative view of Microscolex phosphoreus (Duges, 1837) (Annelida: Clitellata: Acanthodrilidae) / E. Rota [et al.] // Zootaxa. - 2018. - Vol. 4496, Is. 1. - P175-189, DOI 10.11646/zootaxa.4496.1.13. - Cited References:53. - This work was partially supported by Grant 15-04-02695-a from the Russian Foundation for Basic Research and the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351504). Funding for travel to Western Australia (CE, SM) was provided by the Adlerbert Foundation. . - ISSN 1175-5326. - ISSN 1175-5334

РУБ Zoology
Рубрики:
BIOLUMINESCENT EARTHWORMS
   LUMINOUS EARTHWORM
   OLIGOCHAETA
   HAPLOTYPES
Кл.слова (ненормированные):
peregrine earthworm -- cryptic diversity -- morphology -- DNA-barcoding -- bioluminescence
Аннотация: The small synanthropic and peregrine earthworm Microscolex phosphoreus (Duges, 1837) is reported for the first time from Siberia. Morphological and DNA barcode (COI) analyses of this and widely separate samples worldwide demonstrate that, as currently identified, M. phosphoreus is a heterogeneous taxon, with divergent lineages occurring often in the same locality and hardly providing geographically structured genetic signals. The combined morphological and genetic evidence suggests that at least four of the found clades should be reclassified as separate species, both morphologically and genetically distinct from each other. However, as the specimen number was limited and only the COI gene was studied for the genetic work, we hesitate in formally describing new species. There would also be the problem of assigning the available names to specific lineages. Our findings encourage careful external and anatomical examination and using reliable characters such as the interchaetal distances and spermathecal morphology for correct identification and for deeper evaluation of cryptic diversity in this interesting bioluminescent worm.

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Держатели документа:
Univ Siena, Dept Phys Earth & Environm Sci, Via PA Mattioli 4, IT-53100 Siena, Italy.
Univ Gothenburg, Dept Biol & Environm Sci, Systemat & Biodivers, Box 463, SE-40530 Gothenburg, Sweden.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Lab Photobiol, Krasnoyarsk, Russia.

Доп.точки доступа:
Rota, Emilia; Martinsson, Svante; Erseus, Christer; Petushkov, Valentin N.; Rodionova, Natalja S.; Omodeo, Pietro; Russian Foundation for Basic Research [15-04-02695-a]; Adlerbert Foundation; [01201351504]

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18.

Toxic effects of the fungicide tebuconazole on the root system of fusarium-infected wheat plants / E. Shishatskaya [et al.] // Plant Physiol. Biochem. - 2018. - Vol. 132. - P400-407, DOI 10.1016/j.plaphy.2018.09.025 . - ISSN 0981-9428
Кл.слова (ненормированные):
Border cells -- Carbonylated proteins -- Free proline -- Fusarium -- Malondialdehyde -- Tebuconazole
Аннотация: The study investigates toxic effects of the fungicide tebuconazole (TEB) on Fusarium-infected wheat (Triticum aestivum) plants based on the morphological characteristics of root apices and changes in the integrated parameters of redox homeostasis, including the contents of free proline and products of peroxidation of proteins (carbonylated proteins, CP) and lipids (malondialdehyde, MDA) in roots. In two-day-old wheat sprouts infected by Fusarium graminearum, the levels of proline, CP, and border cells of root apices are higher than in roots of uninfected sprouts by a factor of 1.4, 8.0, and 3, respectively. The triazole fungicide tebuconazole (TEB) at the concentrations of 0.01, 0.10, and 1.00 ?g ml?1 of medium causes a dose-dependent decrease in the number of border cells. The study of the effects of TEB and fusarium infection on wheat plants in a 30-day experiment shows that the effect of the fungicide TEB on redox homeostasis in wheat roots varies depending on the plant growth stage and is significantly different in ecosystems with soil and plants infected by Fusarium phytopathogens. The study of the morphology of root apices shows that the toxic effects of TEB and fusarium infection are manifested in the destructive changes in root apices and the degradation of the root tip mantle. © 2018 Elsevier Masson SAS

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Ave., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
International and Interuniversity Centre for Nano Science and Nano Technology, Kottayam, Kerala, India

Доп.точки доступа:
Shishatskaya, E.; Menzyanova, N.; Zhila, N.; Prudnikova, S.; Volova, T.; Thomas, S.

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19.

Development and characterization of ceftriaxone-loaded P3HB-based microparticles for drug delivery / A. V. Murueva [et al.] // Dry. Technol. - 2018, DOI 10.1080/07373937.2018.1487451 . - Article in press. - ISSN 0737-3937
Кл.слова (ненормированные):
Ceftriaxone -- drug release -- emulsification technique -- microbiological evaluation -- P3HB-microparticles -- spray-drying -- Drying -- Emulsification -- Encapsulation -- Functional polymers -- Spray drying -- Targeted drug delivery -- Anti-bacterial activity -- Ceftriaxone -- Double emulsifications -- Drug release -- Encapsulation efficiency -- Micro-particles -- Microbiological evaluation -- Polymeric microparticles -- Controlled drug delivery
Аннотация: In this study, polymer-based microparticles are used to improve the therapeutic properties of ceftriaxone (CEF) and render them safer. Poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (P3HB-PEG)-based microparticles were prepared by two methods: a double emulsification technique and spray-drying. The microparticles were characterized in terms of size and zeta potential, morphology, total drug loading and drug release. The microparticles had spherical shapes with diameters of a size range from 0.74 to 1.55 µm (emulsification technique) and from 3.84 to 6.51 µm (spray-drying); CEF encapsulation efficiency was around 63% and 49% for these methods respectively. The CEF release from microparticles obtained by spray-drying reached 100% after 150 h, while for microparticles obtained by emulsification technique the total release of CEF did not exceed 34% after 312 h. The release profiles could be best explained by Zero order kinetics model, Higuchi and Korsmeyer-Peppas models, as the plots showed high linearity. Antibacterial activity of the microparticles was evaluated against gram-positive and gram-negative bacterial strains. In general, CEF encapsulation in polymeric microparticles preserves the therapeutic efficacy of the CEF and provides its prolonged effect. © 2018, © 2018 Taylor & Francis.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Murueva, A. V.; Shershneva, A. M.; Abanina, K. V.; Prudnikova, S. V.; Shishatskaya, E. I.

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20.

Postglacial Colonization of the North European Seas by Pacific Fishes and Lamprey / A. A. Makhrov, D. L. Lajus // Contemp. Probl. Ecol. - 2018. - Vol. 11, Is. 3. - P247-258, DOI 10.1134/S1995425518030071. - Cited References:134. - This work was supported by Russian Science Foundation, project no. 16-14-10001. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
MULTIPLE GLACIAL REFUGIA
   GENETIC DIFFERENTIATION
   SPECIES COMPLEX
   1956
Кл.слова (ненормированные):
Arctic Ocean -- zoogeography -- phylogeography -- fish -- lamprey -- evolution -- immobilization
Аннотация: A critical analysis of literature data on the distribution, morphology, and phylogeography of the Arctic lamprey (Lethenteron camtschaticum) and five species of marine and anadromous fish such as navaga (Eleginus navaga), pollock (Theragra chalcogramma), rainbow smelt (Osmerus mordax dentex), Pacific herring (Clupea pallasii), and pond smelt (Hypomesus olidus) has been performed. The results show that all these species have colonized Northern European seas, distributing along the Arctic coastline of Eurasia after the glacier retreat. The reasons that the dispersal of these species in the Atlantic Ocean may be impeded (preference for a cold environment, competition, and decrease of the evolutionary potential) are discussed.

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Держатели документа:
Russian Acad Sci, Severtsov Inst Ecol & Evolut, Moscow 119071, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
St Petersburg State Univ, St Petersburg 199178, Russia.

Доп.точки доступа:
Makhrov, A. A.; Lajus, D. L.; Russian Science Foundation [16-14-10001]

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