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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G.
Заглавие : A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures
Колич.характеристики :9 с
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 8. - P915-923. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000036280.98763.c1
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLY(3-HYDROXYBUTYRATE)
POLYESTERS
POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G.
Заглавие : A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 8. - С. 915-923. - 9. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000036280.98763.c1
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLY(3-HYDROXYBUTYRATE)
POLYESTERS
POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Simoncelli S., Kirillin G., Tolomeev A. P., Grossart H. -P.
Заглавие : A low-cost underwater particle tracking velocimetry system for measuring in situ particle flux and sedimentation rate in low-turbulence environments
Место публикации : Limnol. Oceanogr. Methods: Wiley Blackwell, 2019. - Article in press. - ISSN 15415856 (ISSN) , DOI 10.1002/lom3.10341
Аннотация: We describe a low-cost three-dimensional underwater particle tracking velocimetry system to directly measure particle settling rate and flux in low-turbulence aquatic environments. The system consists of two waterproof cameras that acquire stereoscopic videos of sinking particles at 48 frames s?1 over a tunable sampling volume of about 45 ? 25 ? 24 cm. A dedicated software package has been developed to allow evaluation of particle velocities, concentration and flux, but also of morphometric parameters such as particle area, sinking angle, shape irregularity, and density. Our method offers several advantages over traditional approaches, like sediment trap or expensive in situ camera systems: (1) it does not require beforehand particle collection and handling; (2) it is not subjected to sediment trap biases from turbulence, horizontal advection, or presence of swimmers, that may alter particulate load and flux; (3) the camera system enables faster data processing and flux computation at higher spatial resolution; (4) apart from the particle settling rates, the particle size distribution, and morphology is determined. We tested the camera system in Lake Stechlin (Germany) in low turbulence and mean flow, and analyzed the morphological properties and settling rates of particles to determine their sinking behavior. The particle flux assessed from conventional sediment trap measurements agreed well with that determined by our system. By this, the low-cost approach demonstrated its reliability in low turbulence environments and a strong potential to provide new insights into particulate carbon transport in aquatic systems. Extension of the method to more turbulent and advective conditions is also discussed. © 2019 The Authors. Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhrov A. A.
Заглавие : A narrowing of the phenotypic diversity range after large rearrangements of the karyotype in salmonidae: The relationship between saltational genome rearrangements and gradual adaptive evolution
Место публикации : Genes: MDPI AG, 2017. - Vol. 8, Is. 11. - ISSN 20734425 (ISSN) , DOI 10.3390/genes8110297
Ключевые слова (''Своб.индексиров.''): ecology--evolution--genome--karyotype--morphology--ecology--gene rearrangement--genome--karyotype--morphology--nonhuman--salmonid
Аннотация: The problem of how a gradual development of ecological and morphological adaptations combines with large genome rearrangements, which have been found to occur in the phylogeny of many groups of organisms, is a matter of discussion in the literature. The objective of this work was to study the problem with the example of salmonids, whose evolution included at least six events of multiple chromosome fusions. Large karyotype rearrangements are associated with a decrease in ecological and morphological diversity in salmonids. In the above example, genome rearrangements seem to distort the function of the genetic systems that are responsible for the occurrence of certain ecological forms in salmonids. © 2017 by the authors; Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lunina O.N., Bryantseva I.A., Akimov V.N., Rusanov I.I., Barinova E.S., Lysenko A.M., Rogozin D.Yu., Pimenov N.V.
Заглавие : Anoxygenic phototrophic bacterial community of Lake Shira (Khakassia)
Место публикации : Microbiology. - 2007. - Vol. 76, Is. 4. - С. 469-479. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261707040133
Ключевые слова (''Своб.индексиров.''): anoxygenic photosynthesis--anoxygenic phototrophic bacteria--meromictic brackish lakes--ahrensia kielensis--bacteria (microorganisms)--chlorobi--chlorobium--chlorobium limicola--chromatiaceae--lamprocystis purpurea--photobacteria--proteobacteria--rhodomicrobium--rhodovulum--rhodovulum strictum--thiocapsa roseopersicina
Аннотация: The anoxygenic phototrophic bacterial community of the brackish meromictic Lake Shira (Khakassia) was investigated in August 2001, July 2002, and February-March 2003. In all the periods of investigation, the prevailing microorganisms were purple sulfur bacteria similar to Lamprocystis purpurea in morphology and pigment composition. Their highest number (3 ? 10 5 cells/ml) was recorded in July 2002 at the depth of 15 m. According to 16S rRNA gene analysis, the strain of purple sulfur bacteria isolated in 2001 and designated ShAm01 exhibited 98.6% similarity to the type strain of Thiocapsa roseopersicina and 97.1-94.4% similarity to the type strains of Tca. pendens, Tca. litoralis, and Tca. rosea. The minor microorganisms of the anoxygenic phototrophic bacterial community within the period of investigation were nonsulfur purple bacteria phylogenetically close to Rhodovulum strictum (98.3% similarity, strain ShRb01), Ahrensia kielensis (of 93.9% similarity, strain ShRb02), Rhodomicrobium vannieli (of 99.7% similarity, strain ShRmc01), and green sulfur bacteria, phylogenetically close to Chlorobium limicola (of 98.7% similarity, strain ShCl03). В© 2007 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Goreva A., Kalacheva G., Volova T.
Заглавие : Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - С. 2185-2203. - ISSN 09205063 (ISSN) , DOI 10.1163/092050610X537138
Ключевые слова (''Своб.индексиров.''): 14c products of polymer resorption--intravenous administration--microparticles--resorbable polyhydroxybutyrate--tissues of internal organs--3-hydroxybutyric acid--sup14/supc products of polymer resorption--adverse effect--drug-delivery systems--growth and development--high molecular weight--initial molecular weight--initial values--internal organs--intravenous administration--laboratory animals--local response--matrix--mean diameter--micro-particles--polymer degradation--polymer microparticles--preparation process--radioactivity level--residual content--resorbable--sustained-release--tail veins--wistar rat--animals--biocompatibility--degradation--histology--molecular weight--morphology--radiation--radioactivity--rats--tissue--poly(3 hydroxybutyric acid)--animal experiment--animal tissue--article--biocompatibility--concentration process--controlled study--degradation--drug delivery system--female--gel permeation chromatography--heart--kidney parenchyma--liver--lung parenchyma--molecular weight--nonhuman--organ culture--priority journal--radioactivity--rat--spleen--animals--animals, laboratory--biocompatible materials--drug delivery systems--female--infusions, intravenous--materials testing--microspheres--molecular weight--particle size--polymers--rats--rats, wistar--tissue distribution--animalia--rattus--rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Boyandin A. N., Sukhanova A. A., Nikolaeva E. D., Sukovatyi A. G., Nemtsev I. V.
Заглавие : Blends of poly-3-hydroxybutyrate and poly-ε-caprolactone: Morphology, properties and biocompatibility
Место публикации : AIP Conference Proceedings: American Institute of Physics Inc., 2021. - Vol. 2388: 4th International Conference on Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020 (16 November 2020 through 20 November 2020, ) Conference code: 174383. - Ст.020002. - , DOI 10.1063/5.0068677
Аннотация: A series of films was obtained from blends of poly-3-hydroxybutyrate (PHB) and poly-s-caprolactone (PCL). The structure, physical, mechanical and biological properties of the films were studied, depending on the ratio of the components. The microscopic structure of mixed films was determined by the main component of the blend: the films were porous with a predominance of PHB and relatively smooth with a predominance of PCL. The highest values of elongation at break (229-269%), as well as the local maximum values of tensile strength (14-15 MPa) were noted for blends with 5-25% PHB content. At PHB content of 25% and 75%, local maxima of Young's modulus were observed (507 and 655 MPa, respectively). When the ratio of the components in the blend was equal (50:50 by weight), the films were heterogeneous and had the lowest values of mechanical strength (ultimate strength and elongation at break were 3 MPa and 15.5%, respectively). A study of the biocompatibility of mixed films in a culture of NIH 3T3 mouse fibroblasts based on determination of the number of adhered cells in the MTT test showed the absence of cytotoxic action, as well as high adhesion and cell proliferation, in some cases exceeding the data for films from pure PHB and PCL. This demonstrates the suitability of PHB-PCL blends for the production of cell carriers for tissue engineering and other reconstructive technologies. © 2021 Author(s).
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gordeev S.A., Puzyr A.P.
Заглавие : Degradation of P(3HB) and P(3HB-co-3HV) in biological media
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - С. 643-657. - ISSN 09205063 (ISSN) , DOI 10.1163/1568562053783678
Ключевые слова (''Своб.индексиров.''): degradation rate--fiber properties--morphology--poly(hydroxybutyrate-co-hydroxyvalerate) (p(3hb-co-3hv))--polyhydroxyalkanoates(phas)--polyhydroxybutyrate (p(3hb))--copolymers--degradation--differential scanning calorimetry--enzymes--morphology--scanning electron microscopy--tensile strength--tissue--transmission electron microscopy--degradation rate--fiber properties--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (p(3hb))--biopolymers--buffer--copolymer--poly(3 hydroxybutyric acid)--polyhydroxybutyrate hydroxyvalerate copolymer--unclassified drug--animal experiment--animal model--animal tissue--article--biodegradation--controlled study--crystal structure--fiber--giant cell--in vitro study--in vivo study--macrophage--morphology--nonhuman--ph--priority journal--rat--structure analysis--tensile strength--tissue water--weight reduction--animals--biodegradation, environmental--buffers--humans--hydrogen-ion concentration--hydroxybutyrates--macrophages--microscopy, electron, scanning--microscopy, electron, transmission--muscle, skeletal--polyesters--rats--rats, wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Murueva A. V., Shershneva A. M., Abanina K. V., Prudnikova S. V., Shishatskaya E. I.
Заглавие : Development and characterization of ceftriaxone-loaded P3HB-based microparticles for drug delivery
Место публикации : Dry. Technol.: Taylor and Francis Inc., 2018. - Article in press. - ISSN 07373937 (ISSN) , DOI 10.1080/07373937.2018.1487451
Ключевые слова (''Своб.индексиров.''): ceftriaxone--drug release--emulsification technique--microbiological evaluation--p3hb-microparticles--spray-drying--drying--emulsification--encapsulation--functional polymers--spray drying--targeted drug delivery--anti-bacterial activity--ceftriaxone--double emulsifications--drug release--encapsulation efficiency--micro-particles--microbiological evaluation--polymeric microparticles--controlled drug delivery
Аннотация: In this study, polymer-based microparticles are used to improve the therapeutic properties of ceftriaxone (CEF) and render them safer. Poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (P3HB-PEG)-based microparticles were prepared by two methods: a double emulsification technique and spray-drying. The microparticles were characterized in terms of size and zeta potential, morphology, total drug loading and drug release. The microparticles had spherical shapes with diameters of a size range from 0.74 to 1.55 µm (emulsification technique) and from 3.84 to 6.51 µm (spray-drying); CEF encapsulation efficiency was around 63% and 49% for these methods respectively. The CEF release from microparticles obtained by spray-drying reached 100% after 150 h, while for microparticles obtained by emulsification technique the total release of CEF did not exceed 34% after 312 h. The release profiles could be best explained by Zero order kinetics model, Higuchi and Korsmeyer-Peppas models, as the plots showed high linearity. Antibacterial activity of the microparticles was evaluated against gram-positive and gram-negative bacterial strains. In general, CEF encapsulation in polymeric microparticles preserves the therapeutic efficacy of the CEF and provides its prolonged effect. © 2018, © 2018 Taylor & Francis.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lipaikin, Sergei Y., Yaremenko, Ivan A., Terent'ev, Alexander O., Volova, Tatiana G., Shishatskaya, Ekaterina, I
Заглавие : Development of Biodegradable Delivery Systems Containing Novel 1,2,4-Trioxolane Based on Bacterial Polyhydroxyalkanoates
Колич.характеристики :14 с
Коллективы : Government of the Russian Federation [074-02-2018-328]
Место публикации : Adv. Polym. Technol.: WILEY-HINDAWI, 2022. - Vol. 2022. - Ст.6353909. - ISSN 0730-6679, DOI 10.1155/2022/6353909. - ISSN 1098-2329(eISSN)
Примечания : Cited References:70. - \his study was financially supported by the project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning."
Предметные рубрики: IN-VITRO
BRIDGED 1,2,4,5-TETRAOXANES
ANTIMALARIAL ACTIVITY
RELEASE
Аннотация: In this work, delivery systems in the form of microparticles and films containing 1,2,4-trioxolane (ozonide, OZ) based on polyhydroxyalkanoates (PHAs) were developed. Main systems' characteristics were investigated: the particle yield, average diameter, zeta potential, surface morphology, loading capacity, and drug release profile of microparticles, as well as surface morphology and release profiles of OZ-containing films. PHA-based OZ-loaded microparticles have been found to have satisfactory size, zeta potential, and ozonide loading-release behavior. It was noted that OZ content influenced the surface morphology of obtained systems.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vinokurov A.P., Eremeev A.V., Setkov N.A.
Заглавие : Dobutamine prevents experimental postintoxication liver cirrhosis in mice
Место публикации : Bulletin of Experimental Biology and Medicine. - 2002. - Vol. 134, Is. 1. - P43-46. - ISSN 00074888 (ISSN) , DOI 10.1023/A:1020604621142
Ключевые слова (''Своб.индексиров.''): dobutamine--hepatocytes--liver cirrhosis--proliferation--adrenergic receptor stimulating agent--alpha 1 adrenergic receptor stimulating agent--beta 1 adrenergic receptor stimulating agent--beta 2 adrenergic receptor stimulating agent--carbon tetrachloride--catecholamine derivative--dobutamine--epidermal growth factor--animal cell--animal experiment--animal model--animal tissue--article--cell activity--cell culture--cell death--cell proliferation--cell stimulation--chronic hepatitis--connective tissue--controlled study--disease association--drug effect--drug potency--experimental test--female--liver cell--liver cirrhosis--liver fibrosis--liver hyperplasia--liver necrosis--liver parenchyma--liver regeneration--liver toxicity--morphology--mouse--nonhuman--toxicity testing--adrenergic beta-agonists--animals--carbon tetrachloride--cell division--dobutamine--dose-response relationship, drug--female--hepatocytes--liver--liver cirrhosis--mice--mice, inbred balb c--animalia
Аннотация: Various chronic inflammatory and necrotic processes in the liver parenchyma are accompanied by pathological morphofunctional changes, which are associated with hepatocyte death and hyperplasia of the connective tissue. Regeneration of the liver parenchyma should include not only prevention of fibrosis, but also stimulation of hepatocyte prolifearation. The adrenoceptor agonist dobutamine stimulated proliferative activity of cultured hepatocytes and prevented the development of postintoxication liver cirrhosis in mice produced by chronic poisoning with CCl4.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Alimova A.N., Chubun N.N., Belobrov P.I., Detkov P.Y., Zhirnov V.V.
Заглавие : Electrophoresis of nanodiamond powder for cold cathode fabrication
Место публикации : J. Vac. Sci. Technol. B: AMER INST PHYSICS, 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - С. 715-718. - 4. - ISSN 1071-1023, DOI 10.1116/1.590625
Примечания : Cited References: 5
Предметные рубрики: FIELD-EMISSION
TIPS
Аннотация: The process of electrophoresis/dielectrophoresis of shock-synthesized nanodiamond powders on the large-area Si field emission arrays was studied. The process was found to be very sensitive to the modification of nanodiamond powder. The growth rate and film morphology of nanodiamond coatings on Si tips were investigated. Results in fabrication and testing of nanodiamond-based cold cathodes and sealed device prototypes are presented. (C) 1999 American Vacuum Society. [S0734-211X(99)06002-3].
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13.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Y.
Заглавие : Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems
Колич.характеристики :6 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1581-1586. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00249-6
Примечания : Cited References: 17
Предметные рубрики: MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rota, Emilia, Martinsson, Svante, Erseus, Christer, Petushkov, Valentin N., Rodionova, Natalja S., Omodeo, Pietro
Заглавие : Green light to an integrative view of Microscolex phosphoreus (Duges, 1837) (Annelida: Clitellata: Acanthodrilidae)
Колич.характеристики :15 с
Коллективы : Russian Foundation for Basic Research [15-04-02695-a]; Adlerbert Foundation; [01201351504]
Место публикации : Zootaxa: MAGNOLIA PRESS, 2018. - Vol. 4496, Is. 1. - С. 175-189. - ISSN 1175-5326, DOI 10.11646/zootaxa.4496.1.13. - ISSN 1175-5334(eISSN)
Примечания : Cited References:53. - This work was partially supported by Grant 15-04-02695-a from the Russian Foundation for Basic Research and the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351504). Funding for travel to Western Australia (CE, SM) was provided by the Adlerbert Foundation.
Предметные рубрики: BIOLUMINESCENT EARTHWORMS
LUMINOUS EARTHWORM
OLIGOCHAETA
HAPLOTYPES
Ключевые слова (''Своб.индексиров.''): peregrine earthworm--cryptic diversity--morphology--dna-barcoding--bioluminescence
Аннотация: The small synanthropic and peregrine earthworm Microscolex phosphoreus (Duges, 1837) is reported for the first time from Siberia. Morphological and DNA barcode (COI) analyses of this and widely separate samples worldwide demonstrate that, as currently identified, M. phosphoreus is a heterogeneous taxon, with divergent lineages occurring often in the same locality and hardly providing geographically structured genetic signals. The combined morphological and genetic evidence suggests that at least four of the found clades should be reclassified as separate species, both morphologically and genetically distinct from each other. However, as the specimen number was limited and only the COI gene was studied for the genetic work, we hesitate in formally describing new species. There would also be the problem of assigning the available names to specific lineages. Our findings encourage careful external and anatomical examination and using reliable characters such as the interchaetal distances and spermathecal morphology for correct identification and for deeper evaluation of cryptic diversity in this interesting bioluminescent worm.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S.E., Mogil'naya O.A., Popova L.Yu.
Заглавие : Heterogeneity of the populations of marine luminescent bacteria Photobacterium leiognathi under different conditions of cultivation
Место публикации : Microbiology. - 2006. - Vol. 75, Is. 3. - С. 292-299. - ISSN 00262617 (ISSN) , DOI 10.1134/S002626170603009X
Ключевые слова (''Своб.индексиров.''): bioluminescence--luminescent bacteria--morphology of colonies--bacteria (microorganisms)--photobacterium leiognathi
Аннотация: Manifestation of pleiotropic effects in the isogenic variants of the luminescent bacterium Photobacterium leiognathi 54 was investigated. The decrease or increase of the expression level of bioluminescence was caused by changes in lux operon regulation. The dynamics of the bioluminescence of dark and dim variants did not differ from the dynamics of the initial luminescent variant, but dependence of the level of luminescence intensity on the exogenous autoinducer of the lux operon was revealed. The investigated variants of P. leiognathi 54 inherited fairly stable morphological characteristics, colony architectonics, level of luminescence, and activity of some enzymes; variants with reduced bioluminescence formed colonies of the S type. Stable bright variants with S-and R-type colonies appeared both in the initial strain population and in the dark variant population, but with smaller frequency. Populations of the bright variant with R-type colonies were most heterogeneous; this can be determined by the lack of glucose repression of the bioluminescence in contrast to other investigated inherited variants of P. leiognathi. В© Pleiades Publishing, Inc., 2006.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Verschoor A.M., Zadereev Y.S., Mooij W.M.
Заглавие : Infochemical-mediated trophic interactions between the rotifer Brachionus calyciflorus and its food algae
Место публикации : Limnology and Oceanography. - 2007. - Vol. 52, Is. 5. - С. 2109-2119. - ISSN 00243590 (ISSN)
Ключевые слова (''Своб.индексиров.''): alga--aquatic ecosystem--assimilation efficiency--experimental study--feeding behavior--food web--freshwater environment--ingestion rate--trophic interaction--algae--brachionus calyciflorus--rotifera--scenedesmus--scenedesmus obliquus
Аннотация: We studied how chemicals obtained as filtrates from algal monocultures (algal chemicals) and from rotifer cultures with or without algae (rotifer chemicals) affected feeding rates of the rotifer Brachionus calyciflorus on its food algae, both directly and indirectly (through chemical-induced changes in algal morphology). Algal chemicals had a strong stimulating effect on the feeding rate of B. calyciflorus, but these effects were counteracted by rotifer chemicals. In functional response experiments, rotifer chemicals lowered maximum ingestion rates and had strong effects on assimilation rates and assimilation efficiencies of B. calyciflorus, probably due to the release of unspecific (auto)toxic metabolites. Furthermore, rotifer chemicals induced colony formation in the food alga Scenedesmus obliquus. Above the optimum particle size for ingestion by B. calyciflorus, larger algal colony sizes increased the food-handling time, thus lowering ingestion and assimilation rates. Through their effects on trophic interactions, infochemicals may play a role in structuring and the functioning of aquatic food webs. В© 2007, by the American Society of Limnology and Oceanography, Inc.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L., Vlasova I., Mogilnaya O., Bolsunovsky A., Kalmykov S.
Заглавие : Microdistribution of 241Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River
Место публикации : Journal of Environmental Radioactivity. - 2010. - Vol. 101, Is. 1. - С. 16-21. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2009.08.003
Ключевые слова (''Своб.индексиров.''): alpha-track analysis--americium--elodea canadensis--liquid-scintillation spectrometry--microdistribution--alpha-track analysis--elodea canadensis--microdistribution--scintillation spectrometry--track analysis--liquids--luminescence--radioisotopes--scintillation--spectrometry--spectroscopy--americium--americium 241--americium--americium--bioaccumulation--experimental study--leaf--macrophyte--mass spectrometry--morphology--radionuclide--spatial distribution--stem--submerged vegetation--tracking--aquatic flora--article--bioaccumulation--concentration (parameters)--elodea canadensis--environmental radioactivity--isotope analysis--isotope tracing--leaf lamina--leaf surface--macrophyte--nonhuman--plant cell--plant morphology--plant stem--radioisotope distribution--river--chemistry--hydrocharitaceae--metabolism--plant--plant leaf--radiation monitoring--river--water pollutant--eurasia--russian federation--yenisei river--elodea canadensis--americium--hydrocharitaceae--plant leaves--plant shoots--plant stems--radiation monitoring--rivers--water pollutants, radioactive
Аннотация: A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide 241Am among different components of the plant. The total amount of 241Am added to the experimental system was 1850 В± 31 Bq/L. The total amount of 241Am accumulated by the plants was 182 Bq per sample, or 758,333 В± 385 Bq/kg dry mass. It has been found that the major portion of 241Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of 241Am within different components of the submerged plant E. canadensis was not uniform. 241Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem. В© 2009 Elsevier Ltd. All rights reserved.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Borovikova E. A., Budin Y. V.
Заглавие : Morphological and Genetic Diversity of Two Forms of Muksun Coregonus muksun (Salmonidae) of the Khatanga River Basin as a Key for Understanding the Phylogenetic Relationships between Muksun and Whitefish C. lavaretus
Место публикации : J. Ichthyol.: Pleiades journals, 2020. - Vol. 60, Is. 6. - С. 909-922. - ISSN 00329452 (ISSN), DOI 10.1134/S0032945220060016
Аннотация: Abstract: Morphological analysis of muksun Coregonus muksun of the Khatanga River basin revealed that along with its typical high-density-rakered form, individuals of the low-density-rakered form also live in this region. Analysis of the genetic polymorphism of three marker regions of mitochondrial (ND1 and COI fragments) and nuclear (ITS1) DNA indicates the polyphyletic origin of these forms. The revealed low level of genetic differentiation of muksun and whitefish C. lavaretus, along with the available data on the absence of clear diagnostic species characteristics, suggest that they belong to the same biological species C. lavaretus. © 2020, The Author(s).
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Borovikova E. A., Budin, Yu. V.
Заглавие : Morphological and Genetic Diversity of Two Forms of Muksun Coregonus muksun (Salmonidae) of the Khatanga River Basin as a Key for Understanding the Phylogenetic Relationships between Muksun and Whitefish C. lavaretus
Колич.характеристики :14 с
Коллективы : Russian Scientific FoundationRussian Science Foundation (RSF) [16-14-10001]
Место публикации : J. Ichthyol.: PLEIADES PUBLISHING INC, 2020. - Vol. 60, Is. 6. - С. 909-922. - ISSN 0032-9452, DOI 10.1134/S0032945220060016. - ISSN 1555-6425(eISSN)
Примечания : Cited References:52. - The paper is prepared with financial support of the Russian Scientific Foundation, grant no. 16-14-10001.
Предметные рубрики: PHYLOGEOGRAPHY
POPULATION
ALBULA
IDENTIFICATION
PLESHCHEYEVO
MTDNA
Аннотация: Morphological analysis of muksun Coregonus muksun of the Khatanga River basin revealed that along with its typical high-density-rakered form, individuals of the low-density-rakered form also live in this region. Analysis of the genetic polymorphism of three marker regions of mitochondrial (ND1 and COI fragments) and nuclear (ITS1) DNA indicates the polyphyletic origin of these forms. The revealed low level of genetic differentiation of muksun and whitefish C. lavaretus, along with the available data on the absence of clear diagnostic species characteristics, suggest that they belong to the same biological species C. lavaretus.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Borovikova E. A., Kodukhova, J., V, Semenova, A., V
Заглавие : Phenotypic Plasticity and Allometry of Craniological Characters of Anadromous and Lacustrine Forms of Whitefish Coregonus lavaretus (Linnaeus, 1758) as an Indication of the Wrong Species Status of Coregonus pidschian
Колич.характеристики :11 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-10001]
Место публикации : Contemp. Probl. Ecol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 13, Is. 6. - С. 620-630. - ISSN 1995-4255, DOI 10.1134/S1995425520060049. - ISSN 1995-4263(eISSN)
Примечания : Cited References:64. - This article was prepared with financial support from the Russian Science Foundation, grant no. 16-14-10001.
Предметные рубрики: SALMON SALMO-SALAR
SKULL MORPHOLOGY
ARCTIC CHARR
SEA
L.
Аннотация: The variability of craniological characters has been studied for anadromous and lacustrine forms of whitefish Coregonus lavaretus (Linnaeus, 1758) from waterbodies of the Karelian coast of the White Sea. The significant phenotypic plasticity of craniological characters of the whitefish depends on the environmental conditions. Moreover, there is a correlation between indexes of the craniological traits (except for the index of the snout length) and the body and/or head length (allometric effect). These results suggest that it is unreasonable to separate anadromous and lacustrine forms of C. lavaretus as distinct taxonomical units and argues for the invalidity of C. pidschian and C. oxyrinchus species.
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