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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vydryakova G.A., Gusev A.A., Medvedeva S.E.
Заглавие : Effect of organic and inorganic toxic compounds on luminescence of luminous Fungi
Место публикации : Applied Biochemistry and Microbiology. - 2011. - Vol. 47, Is. 3. - С. 293-297. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683811010194
Ключевые слова (''Своб.индексиров.''): armillaria--armillaria borealis--armillaria gallica--armillaria mellea--bacteria (microorganisms)--fungi--omphalotus japonicus
Аннотация: The possibility of the development of the solid phase bioluminescent biotest using aerial mycelium of luminous fungi was investigated. Effect of organic and inorganic toxic compounds (TC) at concentrations from 10 -6 to 1 mg/ml on luminescence of aerial mycelia of four species of luminous fungi-Armillaria borealis (Culture Collection of the Institute of Forest, Siberian Branch, Russian Academy of Sciences), A. mellea, A. gallica, and Lampteromyces japonicus (Fungi Collection of the Botanical Institute, Russian Academy of Sciences)-has been studied. Culture of A. mellea was shown to be most sensitive to solutions of the model TC. It was demonstrated that the sensitivity of the luminous fungi is comparable with the sensitivity of the bacteria that are used for environmental monitoring. Use of the aerial mycelium of luminous fungi on the solid support as a test object is a promising approach in biotesting for the development of continuous biosensors for air monitoring. В© 2011 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Manukovsky N.S., Kovalev V.S.
Заглавие : Effect of biohumus on growth of oyster fungus Pleurotus ostreatus and Agaricus bisporus
Колич.характеристики :4 с
Место публикации : Mikol. Fitopatol.: MEZHDUNARODNAYA KNIGA, 1998. - Vol. 32, Is. 5. - P67-70. - ISSN 0026-3648
Примечания : Cited References: 8
Аннотация: The influence of biohumus on the yeild of oester mushroom has been studied. It was shown, that the highest yeild 9.73 g of dry mass per 100 g of dry substrate mass was an the substrate, containing 25 % of biohumus and 75 % of wheat straw. The possible cause of this effect could arise from increasing phosphorus content in the substrate. The hughest growth rate of champignon mycelium was 2.2-2.4 mm/day on the substrate, containing 94-96 % of wheat grains and 4-6 % of biohumus.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kobzeva T.V., Melnikov A.R., Karogodina T.Y., Zikirin S.B., Stass D.V., Molin Y.N., Rodicheva E.K., Medvedeva S.E., Puzyr A.P., Burov A.A., Bondar V.S., Gitelson J.I.
Заглавие : Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation
Колич.характеристики :8 с
Коллективы : Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29, Is. 7. - С. 703-710. - ISSN 1522-7235, DOI 10.1002/bio.2656. - ISSN 1522-7243
Примечания : Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058).
Предметные рубрики: BIOLUMINESCENCE
COMPONENTS
MECHANISMS
SYSTEM
Ключевые слова (''Своб.индексиров.''): higher luminous fungi--neonothopanus nambi--ionizing irradiation--reactive oxygen species--lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Manukovsky N.S., Kovalev V.S., Gribovskaya I.V.
Заглавие : Growing of Pleurotus florida on substrates with inedible potato biomass
Колич.характеристики :7 с
Место публикации : Mikol. Fitopatol.: MEZHDUNARODNAYA KNIGA, 2002. - Vol. 36, Is. 4. - P48-54. - ISSN 0026-3648
Примечания : Cited References: 14
Аннотация: The possibility to grow oyster mushroom Pleurotus florida Fovose on the substrate prepared from inedible potato biomass was tested. It was shown that mycelium growth rate was increased by 61-69 % after boiling or soaking of inedible potato biomass and mixing it with wheat straw. The increase in growth rate can be accounted for the decrease of potassium content in the substrate from 5,53 % to 0.87-0.88 %. Mixing inedible potato biomass with wheat straw has had a positive impact on the oyster mushroom fruiting and the convertion of spent mushroom compost into biohumus.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dementyev D.V., Zotina T.A., Manukovsky N.S., Kalacheva G.S., Bolsunovsky, A. Ya.
Заглавие : Biosorption of Am-241 from aqueous solutions and its biochemical fractionation in Pleurotus ostreatus mycelium
Колич.характеристики :3 с
Коллективы : Russian Foundation for Basic Research [12-04-00915]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 460, Is. 1. - С. 34-36. - ISSN 1607-6729, DOI 10.1134/S160767291501010X. - ISSN 1608-3091(eISSN)
Примечания : Cited References:11. - This work was supported by the Russian Foundation for Basic Research (project no. 12-04-00915).
Предметные рубрики: SACCHAROMYCES-CEREVISIAE
REMOVAL
AMERICIUM
MECHANISM
BIOMASS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Puzyr A.P., Burov A.E., Medvedeva S.E., Rodicheva E.K., Kobzeva T.V., Melnikov A.R., Karogodina T.Y., Zikirin S.B., Stass D.V., Molin, Yu. N., Gitelson J.I.
Заглавие : Effect of ionizing radiation on the luminescence of mycelium of luminous fungus Neonothopanus nambi
Колич.характеристики :4 с
Коллективы : Support Program for Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences [71], Program of the President of the Russian Federation for Support of Leading Scientific Schools [NSh-5744.2014.3]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 460, Is. 1. - С. 30-33. - ISSN 1607-6729, DOI 10.1134/S1607672915010093. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15. - This work was supported in part by the Support Program for Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences (project no. 71) and the Program of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh-5744.2014.3).
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya, O. A., Ronzhin, N. O., Medvedeva, S. E., Bondar', V. S.
Заглавие : Total peroxidase and catalase activity of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in comparison with the level of light emission
Колич.характеристики :6 с
Коллективы : Siberian Branch of the Russian Academy of Sciences [71]
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 51, Is. 4. - С. 419-424. - ISSN 0003-6838, DOI 10.1134/S0003683815040110. - ISSN 1573-8183(eISSN)
Примечания : Cited References:35. - The authors are grateful to N. V. Psurtseva (curator of the collection of basidiomycetes of the Botanical Institute, Russian Academy of Science) for help with the species affiliation of the IBSO 2328 culture. This work was supported by the Program of Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences, project no. 71.
Предметные рубрики: OXIDATIVE STRESS
SYSTEM
FUNGI
BIOLUMINESCENCE
LUMINESCENCE
Ключевые слова (''Своб.индексиров.''): basidiomycetes--luminescence--peroxidase--catalase
Аннотация: The peroxidase and catalase activities in the mycelium of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in normal conditions and under stress were compared. An increase in the luminescence level was observed under stress, as well as an increase in peroxidase and catalase activities. Moreover, the peroxidase activity in extracts of A. borealis mycelium was found to be almost one and a half orders of magnitude lower, and the catalase activity more than two orders of magnitude higher in comparison with the N. nambi mycelium. It can be suggested that the difference between the brightly luminescent and dimly luminescent mycelium of N. nambi is due to the content of (HO2)-O-2 or other peroxide compounds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S. E., Artemenko K. S., Krivosheenko A. A., Rusinova A. G., Rodicheva E. K., Puzyr A. P., Bondar V. S.
Заглавие : Growth and light emission of luminous basidiomycetes cultivated on solid media and in submerged culture
Колич.характеристики :13 с
Коллективы : RF Government [11.G34.31.058]; SB RAS [71, 38]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2014. - Vol. 5, Is. 4. - С. 565-577. - ISSN 2077-7000, DOI 10.5943/mycosphere/5/4/9
Примечания : Cited References:23. - This study was supported by grant No. 11.G34.31.058 (RF Government) and Projects No. 71 and No. 38 (SB RAS).
Предметные рубрики: MYCELIAL GROWTH
PANELLUS-STYPTICUS
BIOLUMINESCENCE
LUMINESCENCE
Ключевые слова (''Своб.индексиров.''): luminescence--luminous higher fungi--mycelium
Аннотация: There are higher fungi that emit visible light; however, little is known about their requirements for good growth and bright luminescence. Knowledge of these requirements is extremely important for maintaining fungal cultures in laboratory conditions and preparation of luminous mycelia for research purposes. Luminous higher fungi Panellus stipticus, Armillaria sp. and Neonothopanus nambi isolated from different climatic areas and maintained in CCIBSO 836 (Collection of IBP SB RAS, Russia) were used for experiments. Techniques for static and submerged cultivation of mycelia of higher fungi have been developed and optimized for the production of samples of aerial and globular mycelia with prolonged and stable luminescence. We investigated the growth characteristics and luminescence of mycelia cultivated in/on different nutrient media, and the effects of deionized water and mechanical damage on the light emission of mycelia. An increase in luminescence intensity of fungal mycelia can be obtained during cultivation of fungi on a nutrient medium with a certain composition. A significant increase in light emission from N. nambi mycelium can also be obtained after its incubation in water and mechanical damage. The light emission from N. nambi mycelium was greatly enhanced after these treatments, in contrast to the mycelia of Armillaria sp. or P. stipticus. Cultivation conditions that enable growing mycelia with high levels of luminescence will expedite further studies to gain a better understanding of fungal bioluminescence.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dementyev D. V., Zotina T. A., Manukovsky N. S., Kalacheva G. S.
Заглавие : Biosorption of 241Am from solution and its biochemical fractionation in the mycelium of macromycetes
Место публикации : Radiochemistry. - 2015. - Vol. 57, Is. 6. - С. 661-665. - ISSN 10663622 (ISSN) , DOI 10.1134/S1066362215060144
Ключевые слова (''Своб.индексиров.''): americium--fungus mycelium--sorption
Аннотация: Experiments with macromycetes Pleurotus ostreatus, Neonothopanus nambi, and Agaricus bisporus demonstrated for the first time that live mycelium of mushrooms, cultivated on a liquid culture medium, can efficiently take up dissolved 241Am in its biomass. Biochemical fractionation of the mycelium biomass demonstrated for the first time that the major fraction (up to 90%) of 241Am accumulated in the mycelium is bonded to structural polysaccharides of the cell walls. The sorption capacity of cell wall polysaccharides for 241Am is 2.7-3.6 times higher than that of the initial mycelium biomass, and the activity concentration of 241Am in the polysaccharides reached 950 Bq g-1 dry weight. © 2015 Pleiades Publishing, Inc.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Bondar V. S.
Заглавие : The use of glowing wood as a source of luminescent culture of fungus mycelium
Колич.характеристики :17 с
Коллективы : RF Government [11.G34.31.0058]; SB RAS [71]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2016. - Vol. 7, Is. 1. - С. 1-17. - ISSN 2077-7000, DOI 10.5943/mycosphere/7/1/1
Примечания : Cited References:22. - The authors are grateful to Prof. A. Frank, Director of North Borneo Biostation, for the opportunity to carry out studies of glowing wood; to Nadezhda N. Kudashova, a senior researcher at the Institute of Biology and Biophysics at the Tomsk University, for identifying the species of nonluminous fungi. This study was supported by grant no. 11.G34.31.0058 (RF Government) and Projects no. 71 (SB RAS).
Предметные рубрики: BIOLUMINESCENCE CHARACTERISTICS
NEONOTHOPANUS-NAMBI
LIGHT-EMISSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--culture of luminous mycelia--kinetics of luminescent--reaction--light emitting wood--luminous fungus
Аннотация: In studies of fungal bioluminescence, not only fruiting bodies and spores of the fungus, but also samples of luminescent wood, leaf litter or soil may need to be used to derive pure mycelial culture. This study describes an approach to isolating the culture of luminescent fungal mycelium from samples of light-emitting wood found on Borneo Island in November-December 2013. A GelDoc XR Imaging System (Bio-Rad Laboratories, Inc., U.S.) was used for the first time to monitor luminescence and select luminous samples. This study shows that for successful isolation of the culture of luminescent mycelium out of the luminescent wood found in the forest, it is imperative to keep the samples moist (mycelium alive until there is water), while immediate and aseptic delivery of the samples to the laboratory is not a crucial condition (inner layers of wood is "sterile"). Investigation of the growth features of the isolated mycelium in various growing conditions revealed some peculiar properties of its luminescence in comparison with the known luminescent cultures of basidiomycetes. When grown on solid nutrient media, mycelium exhibits low growth rates, long-lasting luminescence (140 days or longer), and emergence and disappearance of local zones with high levels of light emission. Mycelium produced in submerged culture does not emit light, and this effect must be caused by the absence or a very low level of the luminescent reaction substrate in the biomass. The luminescence system isolated from mycelial biomass did not induce luminescent reaction in vitro upon the addition of NADPH (recording intensity is 60 100 URL/sec). We found that enzymes of the luminescence systems isolated from mycelium pellets retained their activity and catalyzed luminescent reaction when a hot extract of the luminous fungus Armillaria sp. (IBSO 2360) was added (near 1900 URL/sec). The same effect was obtained after addition of hot extracts from the fruiting bodies of nonluminous higher fungi Pholiota squarrosa, Cortinarius sp., Hypholoma capnoides and Chroogomphus rutilus (near 3500 URL/sec). The pure culture of luminescent mycelium has been registered in the Culture Collection of IBP SB RAS as IBSO 2371; now it can be used for various in vivo and in vitro studies, including identification of the fungus.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E.
Заглавие : Luminescence of wood samples during long-term storage
Место публикации : Mycosphere: Guizhou Key Laboratory of Agricultural Biotechnology, 2016. - Vol. 7, Is. 6. - С. 716-727. - ISSN 20777000 (ISSN) , DOI 10.5943/mycosphere/7/6/2
Ключевые слова (''Своб.индексиров.''): light emitting wood--luminous mycelia
Аннотация: The present study describes changes in the mycelium of the fungus growing on the luminescent wood collected on Borneo Island in early December 2013 that occurred during 31 months of storage. The study shows that wood samples retain their ability to emit light, forming two types of luminescent mycelium: surface mycelium and aerial mycelium. The hyphae of the surface mycelium form on the surface of the wood sample and then spread over the surface of the polyethylene bag or over the surface of the bottom of tissue culture flasks containing the samples. The aerial mycelium develops later and only in tissue culture flasks, forming biomass composed of local interlaced hyphae, growing upward. The surface mycelium is characterized by non-uniform "flickering" luminescence along the hyphae. There is no diurnal periodicity in the luminescence of this fungus, but luminescence is increased by mechanical disturbance or exposure to ultraviolet radiation. The local impact of these factors causes an increase in luminescence of the mycelium regions that have not been directly affected. It has been assumed that the variable level of luminescence is an individual trait of this fungus species. The results obtained in this study suggest that luminescent wood found on Borneo Island contains mycelium of a fungus species, whose luminescent properties are essentially different from those of the fungi described in the scientific literature.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Bondar V. S.
Заглавие : Biochemical changes causes lack of bioluminescence in fruiting bodies of Armillaria
Место публикации : Mycosphere: Guizhou Key Laboratory of Agricultural Biotechnology, 2017. - Vol. 8, Is. 1. - С. 9-17. - ISSN 20777000 (ISSN) , DOI 10.5943/mycosphere/8/1/2
Ключевые слова (''Своб.индексиров.''): enzymes and substrate of luminescent reaction--kinetics of luminescence--luminous mycelia--nonluminous fruiting bodies of fungus
Аннотация: Mycelium of Armillaria species exhibit bioluminescence in nature and when cultivated on artificial nutrient media. However, fruiting bodies do not emit visible light. The present study investigates biochemical changes which cause this phenomenon. Light emission was studied in experiments with mixtures of cold and hot extracts of the luminous mycelium of Armillaria borealis IBSO 2328 and nonluminous fruiting bodies of this fungus and an unidentified species of the genus (Armillaria sp.). Hot extracts of fruiting bodies of the nonluminous Pholiota squarrosa were used as the substrate analog of the luminescent reaction, as previously this fungus had been found to contain a high amount of this substance. Control experiments showed that cold extracts of A. borealis IBSO 2328 mycelium contained enzymes for the luminescent reaction, which is initiated after addition hot extracts of P. squarrosa fruiting bodies. Parallel experiments with extracts of the fruiting bodies of Armillaria showed that: (i) - cold extracts did not contain enzymes of the luminescent reaction or contain very small amounts of these enzymes and (ii) - hot extracts did not contain substrate of the luminescent reaction. Thus, the reason why fruiting bodies of Armillaria do not emit light is that they do not contain components required for visible luminescence. The study discusses possible causes why the enzymes and substrate of the luminescent reaction are not synthesized in fruiting bodies of Armillaria. © Guizhou Academy of Agricultural Sciences.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K. V., Petushkov V. N., Rodionova N. S., Gitelson J. I.
Заглавие : Why does the bioluminescent fungus Armillaria mellea have luminous mycelium but nonluminous fruiting body?
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 474, Is. 1. - С. 217-219. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672917030176
Аннотация: By determining the components involved in the bioluminescence process in luminous and nonluminous organs of the honey fungus Armillaria mellea, we have established causes of partial luminescence of this fungus. The complete set of enzymes and substrates required for bioluminescence is formed only in the mycelium and only under the conditions of free oxygen access. Since the synthesis of luciferin precursor (hispidin) and 3-hydroxyhispidin hydroxylase in the fruiting bodies is blocked, the formation of luciferin—the key component of fungal bioluminescent system—was not observed. That is why the fruiting body of Armillaria mellea is nonluminous despite the presence of luciferase, the enzyme that catalyzes the oxidation of luciferin with a photon emission. © 2017, Pleiades Publishing, Ltd.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A. P., Medvedeva S. E., Artemenko K. S., Bondar V. S.
Заглавие : Luminescence of cold extracts from mycelium of luminous basidiomycetes during long-term storage
Место публикации : Curr. Res. Environ. Appl. Mycol. J. Fungal: Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection, 2017. - Vol. 7, Is. 3. - С. 227-235. - ISSN 22292225 (ISSN) , DOI 10.5943/cream/7/3/9
Ключевые слова (''Своб.индексиров.''): armillaria borealis--kinetics of luminescence--lyophilic preparations--mycena citricolor--neonothopanus nambi
Аннотация: Cold extracts with high activities of enzymes of luminescent reaction were prepared from mycelia of luminous fungi Armillaria borealis IBSO 2328, Mycena citricolor IBSO 2331, and Neonothopanus nambi IBSO 2391. The authors describe techniques of preparing cold extracts with high levels of luminescence from mycelial biomass of different species of luminous basidiomycetes. The investigation of cold extracts showed that in experiments with freezing and thawing of the samples as well as in experiments with lyophilization followed by dissolution of the dry samples, the levels of enzyme activity were high, with in vitro luminescence exhibited after addition of NADPH and the hot extract containing the substrate. High activity levels of the enzymes of luminescent reaction were measured in lyophilized cold extracts stored over three years. In lyophilized preparations, the enzymes of luminescent reaction had high thermostability, even when dry preparations of cold extracts were exposed to a temperature of 100°C for 60 minutes. © Beijing Academy of Agriculture and Forestry Sciences.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Artemenko K. S., Bondar V. S.
Заглавие : Morphological properties and levels of extracellular peroxidase activity and light emission of the basidiomycete Armillaria borealis treated with beta-glucosidase and chitinase
Колич.характеристики :12 с
Коллективы : Russian Academy of Sciences [0356-2016-0709, 0356-2015-0103]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2017. - Vol. 8, Is. 4. - С. 649-+. - ISSN 2077-7000, DOI 10.5943/mycosphere/8/4/11
Примечания : Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project no. 0356-2016-0709) and Program No. II. 2 "Integration and Development" of the Siberian Branch of the Russian Academy of Sciences (project no. 0356-2015-0103).
Предметные рубрики: FUNGAL CELL-WALL
OXIDATIVE STRESS
PHANEROCHAETE-CHRYSOSPORIUM
Ключевые слова (''Своб.индексиров.''): basidiomycetes--bioluminescence--cell wall--beta-glucosidase--chitinase--peroxidase
Аннотация: The study estimates morphological properties and levels of extracellular peroxidase activity and light emission of mycelium of the basidiomycete Armillaria borealis IBSO 2328 treated with beta-glucosidase and chitinase. Mycelium incubated with the enzymes shows considerable morphological changes and indications of osmotic shock. Injuries observed in the cell envelope of the fungal hyphae are primarily attributed to the partial (in the beta-glucosidase treatment) or complete (in the chitinase treatment) disintegration of the melanin layer on the surface of the cell wall. Changes in the cell wall of hyphae are accompanied by release of extracellular peroxidases of the fungus into the incubation medium and an increase in light emission relative to the luminescence of the control pellets. We assume that higher level of luminescence of the enzyme-treated mycelium samples could be related to the disintegration of the surface pigment layer of the hyphae and the partial loss of extracellular peroxidases. The data obtained confirm the previously proposed hypothesis in which light producing reaction of the fungus may be an additional way to neutralize active oxygen radicals under stress.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Bondar V. S.
Заглавие : Estimating levels of light emission and extracellular peroxidase activity of mycelium of luminous fungus Neonothopanus nambi treated with ?-glucosidase
Место публикации : Curr. Res. Environ. Appl. Mycol. J. Fungal: Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection, 2018. - Vol. 8, Is. 1. - С. 75-85. - ISSN 22292225 (ISSN) , DOI 10.5943/cream/8/1/6
Ключевые слова (''Своб.индексиров.''): basidiomycetes--cell wall--luminescence--polysaccharide sheath
Аннотация: The present study estimates the level of extracellular peroxidase activity and light emission intensity of mycelium of luminescent basidiomycete Neonothopanus nambi treated with ?-glucosidase. A hypothesis has been proposed that treatment with ?-glucosidase may trigger biochemical mechanisms of activation of ROS (primarily hydrogen peroxide) generation in N. nambi mycelium. The results obtained indicate that the enzyme causes partial disintegration of the slimy sheath of fungal hyphae and intracellular matrix, which leads to release of the extracellular peroxidases to the incubation medium. Mycelial cells treated with the enzyme reach the peak of their luminescence sooner. It has been assumed that partial loss of extracellular peroxidases, as important enzymes of antioxidant defense, may be compensated for by an increase in the level of light emission by the fungus. © 2018 Beijing Academy of Agriculture and Forestry Sciences.
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17.

Вид документа : Статья из журнала
Шифр издания : 577.151.45/П 65
Автор(ы) : Пуртов К.В., Петушков В.Н., Родионова Н.С., Гительзон И.И.
Заглавие : Почему у биолюминесцентного опёнка осеннего (Armillaria mellea) светится мицелий, но не светится плодовое тело? : статья
Место публикации : Доклады Академии наук. - Москва: Федеральное государственное унитарное предприятие Академический научно-издательский, производственно-полиграфический и книгораспространительский центр Наука, 2017. - Т. 474, № 4. - С. 510-512. - ISSN 0869-5652, DOI 10.7868/S0869565217040247
УДК : 577.151.45
Аннотация: С помощью определения компонентов-участников биолюминесцентного процесса в светящихся и несветящихся органах опёнка осеннего выяснили причины неполного свечения Armillaria mellea. Полный набор ферментов и субстратов, необходимых для биолюминесценции, образуется только в мицелии, причём исключительно в условиях свободного доступа кислорода. В плодовых телах синтез предшественника люциферина (гиспидина) и 3-гидроксигиспидингидроксилазы блокирован, вследствие этого и не происходило образование люциферина - ключевого компонента биолюминесцентной системы грибов. Именно поэтому плодовое тело опёнка осеннего не светится, несмотря на наличие люциферазы - фермента, катализирующего окисление люциферина с испусканием кванта света.
РИНЦ
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Asanova A. A., Polonskiy V. I., Manukovsky N. S., Khizhnyak S. V.
Заглавие : Fungistatic Activity of Engineered Nanoparticles
Место публикации : Nanotechnologies Russ.: Pleiades Publishing, 2018. - Vol. 13, Is. 5-6. - С. 277-280. - ISSN 19950780 (ISSN) , DOI 10.1134/S1995078018030023
Ключевые слова (''Своб.индексиров.''): cultivation--fungi--silver compounds--silver nanoparticles--titanium dioxide--bipolaris sorokiniana--engineered nanoparticles--high resistance--mycelium growth--nanoparticle concentrations--phytopathogenic fungi--pleurotus ostreatus--sio2 nanoparticles--silica nanoparticles
Аннотация: The influence of various concentrations of Ag, TiO2, and SiO2 nanoparticles on conidia germination of the phytopathogenic fungus Bipolaris sorokiniana and mycelium growth of the xylotrophic fungi Pleurotus ostreatus and Neonothopanus nambi was analyzed. It is established that a decrease in conidia germination of phytopathogenic fungus and mycelium growth of the xylotrophic fungi occurs at relatively high silver nanoparticle concentrations. The appropriate ЕС50 values for Bipolaris sorokiniana, Pleurotus ostreatus, and Neonothopanus nambi are found to be 30, 14, and 31 mg/dm3, respectively. No effect of various concentrations of TiO2 and SiO2 nanoparticles on the studied cultures of fungi is highlighted. Thus, the negative impact of nanosilver on the growth of fungi at a high concentration which is untypical for objects of the environment and a lack of this influence of titanium dioxide and silica nanoparticles testifies to the high resistance of mycelial forms of organisms to engineered nanoparticles. © 2018, Pleiades Publishing, Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Bondar V. S.
Заглавие : Extracellular peroxidase activity and light emission of the mycelium of the basidiomycete neonothopanus nambi in the presence of ?-glucosidase
Место публикации : Biophysics: Pleiades Publishing, 2018. - Vol. 63, Is. 1. - С. 93-99. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350918010104
Ключевые слова (''Своб.индексиров.''): basidiomycetes--cell wall--luminescence--peroxidase--?-glucosidase
Аннотация: A comparative evaluation of the level of extracellular peroxidase activity and light-emission intensity of the mycelium of the luminescent basidiomycete Neonothopanus nambi in the presence of ?-glucosidase was performed. The enzyme activity damages the hyphae of the fungus leading to osmotic imbalance, partial degradation of the mycelium, and release of extracellular peroxidases into the incubation medium. The presence of ?-glucosidase reduces the time necessary to reach the maximum luminescence. Putative biochemical mechanisms that underlie the stimulation of reactive oxygen species formation (first and foremost, of hydrogen peroxide) in the N. nambi mycelium in the presence of ?-glucosidase are proposed. © O.A. Mogilnaya, N.O. Ronzhin, V.S. Bondar and Pleiades Publishing, Inc., 2018.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Mogilnaya O. A., Artemenko K. S., Posokhina E. D., Bondar V. S.
Заглавие : Extracellular Oxidases of Basidiomycete Neonothopanus nambi: Isolation and Some Properties
Колич.характеристики :5 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 490, Is. 1. - С. 38-42. - ISSN 1607-6729, DOI 10.1134/S1607672920010135. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15
Предметные рубрики: PEROXIDASE-ACTIVITY
LIGHT-EMISSION
Аннотация: Using the original technique of treating biomass with beta-glucosidase, a pool of extracellular fungal enzymes was obtained for the first time from the mycelium of basidiomycete Neonothopanus nambi. Two protein fractions containing enzymes with oxidase activity were isolated from the extract by gel-filtration chromatography and conventionally called F1 and F2. Enzyme F1 has a native molecular weight of 80-85 kDa and does not contain chromophore components; however, it catalyzes the oxidation of veratryl alcohol with K-m = 0.52 mM. Probably, this enzyme is an alcohol oxidase. Enzyme F2 with a native molecular weight of approximately 60 kDa is a FAD-containing protein. It catalyzes the cooxidation of phenol with 4-aminoantipyrine without the addition of exogenous hydrogen peroxide, which distinguishes it from the known peroxidases. It was assumed that this enzyme may be a mixed-function oxidase. F2 oxidase has K-m value 0.27 mM for phenol. The temperature optimums for oxidases F1 and F2 are 22-35 and 55-70 degrees C, and pH optimums are 6 and 5, respectively.
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