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Assessing the reliability of quantitative fatty acid signature analysis and compound-specific isotope analysis-based mixing models for trophic studies / I. Prokopkin, O. Makhutova, E. Kravchuk [et al.] // Biomolecules. - 2021. - Vol. 11, Is. 11. - Ст. 1590, DOI 10.3390/biom11111590 . - ISSN 2218-273X
Кл.слова (ненормированные):
CSIA?based mixing model -- Daphnia -- Fatty acids -- Food -- IsoError -- QFASA -- fatty acid -- algal cell culture -- animal experiment -- Article -- Chlorella -- compound specific isotope analysis -- controlled study -- Cryptomonas -- Daphnia -- fatty acid analysis -- gas chromatography -- isotope analysis -- lipid composition -- mathematical model -- nonhuman -- quantitative fatty acid signature analysis -- reliability -- zooplankton
Аннотация: The study of the trophic relationships of aquatic animals requires correct estimates of their diets. We compared the quantitative fatty acid signature analysis (QFASA) and the isotope?mixing model IsoError, based on the compound?specific isotope analysis of fatty acids (CSIA?FA), which are potentially effective models for quantitative diet estimations. In a 21?day experiment, Daphnia was fed a mixture of two food items, Chlorella and Cryptomonas, which were supplied in nearly equal proportions. The percentages and isotope values of the FAs of the algal species and Daphnia were measured. The IsoError based on CSIA?FA gave an estimation of algae consumption using only one FA, 18:3n?3. According to this model, the proportion of consumption of Chlorella decreased while the proportion of consumption of Cryptomonas increased during the experiment. The QFASA model was used for two FA subsets—the extended?dietary subset, which included sixteen FAs, and the dietary one, which included nine FAs. According to both subsets, the portion of consumed Chlorella decreased from Day 5 to 10 and then increased at Day 21. The comparison of the two model approaches showed that the QFASA model is a more reliable method to determine the contribution of different food sources to the diet of zooplankton than the CSIA?based mixing model. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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Держатели документа:
Institute of Biophysics, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Prokopkin, I.; Makhutova, O.; Kravchuk, E.; Sushchik, N.; Anishchenko, O.; Gladyshev, M.

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Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia / Y.-T. Wu, P.-W. Chiang, K. Tandon [et al.] // Microb. Genomics. - 2021. - Vol. 7, Is. 12. - Ст. 000712, DOI 10.1099/mgen.0.000712 . - ISSN 2057-5858
Кл.слова (ненормированные):
Flow cytometry -- Lake Shunet -- Purple sulfur bacteria -- Single-cell genomics -- genomic DNA -- RNA 16S -- Article -- bioinformatics -- carbon metabolism -- Enterobacter -- fluorescence activated cell sorting -- gene amplification -- gene ontology -- high throughput sequencing -- metagenomics -- microbial community -- microbial diversity -- molecular genetics -- nitrogen metabolism -- nonhuman -- nucleotide sequence -- phylogenetic tree -- phylogeny -- polymerase chain reaction -- Sanger sequencing -- Thiocapsa
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.

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Держатели документа:
Department of Forestry, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan
Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Wu, Y. -T.; Chiang, P. -W.; Tandon, K.; Rogozin, D. Y.; Degermendzhy, A. G.; Tang, S. -L.

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Biomonitoring of radioactive contamination of the Yenisei River using aquatic plants / A. Bolsunovsky, D. Dementyev, E. Trofimova // J. Environ. Radioact. - 2020. - Vol. 211. - Ст. 106100, DOI 10.1016/j.jenvrad.2019.106100. - Cited References:39. - The authors are grateful to researchers T. Zotina, M. Medvedeva, A. Zueva, E. Iniatkina, and Yu. Kladko of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) for their assistance in collecting plant samples and conducting cytogenetic analysis. The study was partly supported by grant of the Krasnoyarsk Regional Fund of Science - Russian Foundation for Basic Research No. 18-44-240001. . - ISSN 0265-931X. - ISSN 1879-1700

РУБ Environmental Sciences
Рубрики:
GAMMA-EMITTING RADIONUCLIDES
ELODEA-CANADENSIS
NONHUMAN BIOTA
Кл.слова (ненормированные):
The Yenisei river -- Aquatic plants -- Artificial radionuclides -- The dose -- rate -- Elodea canadensis -- The frequency of chromosomal aberrations
Аннотация: The Yenisei River is contaminated by artificial radionuclides released by one of the Russian facilities producing weapons-grade plutonium (the Mining-and-Chemical Combine, MCC), which has been in operation for 60 years. The paper presents results of long-term monitoring of radionuclide concentrations in six aquatic plant species collected from the 1400 km Yenisei River stretch downstream of the city of Krasnoyarsk. Before the last MCC reactor was shut down (in 2010), up to 30 artificial radionuclides were detected in the plant biomass, and 2 and 5 years after the reactor shutdown, 11 and 3-5 radionuclides, respectively, were detected. The highest concentrations of radionuclides were recorded in Fontinalis antipyretica. The aquatic plant Elodea canadensis, which commonly occurs in the Yenisei River, was used in the cytogenetic study. High frequencies of chromosomal aberrations (up to 33%) were revealed in cells of Elodea canadensis roots from the Yenisei region affected by the MCC radioactive discharge, at dose rates of 45-72 mu Gy/d, while in the cells of the plant roots from the reference areas, the frequency of chromosomal aberrations was 5-7%, at dose rates below 2 mu Gy/d. The higher frequencies of chromosomal aberrations in Elodea canadensis collected from the parts of the River with increased concentrations of artificial radionuclides are associated with the radiation factor. The aquatic plant Elodea canadensis can be recommended as a bio-indicator for radioactively contaminated aquatic ecosystems.

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Держатели документа:
Russian Acad Sci, Radioecol Lab, Siberian Branch, Inst Biophys, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Bolsunovsky, Alexander; Dementyev, Dmitry; Trofimova, Elena; Krasnoyarsk Regional Fund of Science - Russian Foundation for Basic Research [18-44-240001]

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Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system / D. Chang, Y. Liu, Y. Chen [et al.] // BMC Vet. Res. - 2020. - Vol. 16, Is. 1. - Ст. 202, DOI 10.1186/s12917-020-02422-3 . - ISSN 1746-6148
Кл.слова (ненормированные):
Baculovirus expression system -- Canine parvovirus -- VP2 protein -- canine parvovirus vaccine -- protein VP2 -- recombinant protein -- unclassified drug -- virus antibody -- virus vaccine -- affinity chromatography -- animal experiment -- antibody titer -- Article -- baculovirus expression system -- Canine parvovirus -- controlled study -- DNA transposition -- enzyme linked immunosorbent assay -- female -- fluorescence microscopy -- gene expression level -- hemagglutination inhibition -- hemagglutination inhibition test -- immunogenicity -- mouse -- nonhuman -- parvovirus infection -- protein expression -- Sf9 cell line -- vaccination -- Western blotting
Аннотация: Background: Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results: The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions: Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection. © 2020 The Author(s).

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Держатели документа:
Henan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, Key Laboratory of Ecological Security, Collab. Innov. Ctr. of Water Secty. for Water Src. Reg. of Mid-line of S.-to-N. Diversion Proj. of Henan Prov., School of Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China
Institute of Biophysics, Siberian Branch, Russian Academy of Science, Federal Research Center Krasnoyarsk Science Center SB RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Chang, D.; Liu, Y.; Chen, Y.; Hu, X.; Burov, A.; Puzyr, A.; Bondar, V.; Yao, L.

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The smallest isoform of Metridia longa luciferase as a fusion partner for hybrid proteins / M. D. Larionova, S. V. Markova, N. V. Tikunova, E. S. Vysotski // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 14. - Ст. 4971. - P1-16, DOI 10.3390/ijms21144971 . - ISSN 1661-6596
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Copepod luciferase -- Immunoassay -- Single-chain antibody -- Tick-borne encephalitis virus -- fusion protein -- glycoprotein -- histidine -- messenger RNA -- Metridia longa luciferase -- recombinant protein -- single chain fragment variable antibody -- unclassified drug -- amino terminal sequence -- antibody affinity -- antigen binding -- Article -- binding assay -- binding site -- bioluminescence -- bioluminescence resonance energy transfer -- cross reaction -- dissociation constant -- enzyme activity -- Escherichia coli -- gene -- genetic engineering -- genetic transfection -- immunoassay -- limit of detection -- mluc7 gene -- molecular cloning -- nonhuman -- nucleotide sequence -- protein expression -- protein purification -- protein unfolding -- spectral sensitivity -- tick borne encephalitis -- Tick borne encephalitis virus
Аннотация: Bioluminescent proteins are widely used as reporter molecules in various in vitro and in vivo assays. The smallest isoform of Metridia luciferase (MLuc7) is a highly active, naturally secreted enzyme which, along with other luciferase isoforms, is responsible for the bright bioluminescence of marine copepod Metridia longa. In this study, we report the construction of two variants of a hybrid protein consisting of MLuc7 and 14D5a single-chain antibody to the surface glycoprotein E of tick-borne encephalitis virus as a model fusion partner. We demonstrate that, whereas fusion of a single-chain antibody to either N-or C-terminus of MLuc7 does not affect its bioluminescence properties, the binding site on the single-chain antibody influences its binding capacity. The affinity of 14D5a-MLuc7 hybrid protein (KD = 36.2 nM) where the C-terminus of the single-chain antibody was fused to the N-terminus of MLuc7, appeared to be 2.5-fold higher than that of the reverse, MLuc7-14D5a (KD = 87.6 nM). The detection limit of 14D5a-MLuc7 hybrid protein was estimated to be 45 pg of the recombinant glycoprotein E. Although the smallest isoform of M. longa luciferase was tested as a fusion partner only with a single-chain antibody, it is reasonable to suppose that MLuc7 can also be successfully used as a partner for genetic fusion with other proteins. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation

Доп.точки доступа:
Larionova, M. D.; Markova, S. V.; Tikunova, N. V.; Vysotski, E. S.

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Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB) / T. G. Volova [et al.] // Int. J. Biol. Macromol. - 2019. - Vol. 131. - P230-240, DOI 10.1016/j.ijbiomac.2019.03.068. - Cited By :1 . - ISSN 0141-8130
Кл.слова (ненормированные):
Bacterial cellulose -- Composites -- Polyhydroxyalkanoates -- 3 hydroxybutyric acid 4 hydroxybutyric acid copolymer -- actovegin -- bacterial cellulose -- cellulose -- collagen type 1 -- copolymer -- cytokeratin 10 -- cytokeratin 14 -- solcoseryl -- unclassified drug -- angiogenesis -- animal cell -- animal experiment -- animal model -- animal tissue -- biochemical analysis -- biocompatibility -- burn -- cell growth -- controlled study -- cutaneous parameters -- differential scanning calorimetry -- drug release -- elemental analysis -- female -- fibroblast -- histopathology -- in vitro study -- in vivo study -- inflammation -- mechanics -- nonhuman -- rat -- Review -- wound healing -- wound planimetry -- X ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizan Zheleznyak Street, Krasnoyarsk, 660022, Russian Federation

Доп.точки доступа:
Volova, T. G.; Shumilova, A. A.; Nikolaeva, E. D.; Kirichenko, A. K.; Shishatskaya, E. I.

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Role of Hsp90 and ATP in modulating apyrase activity and firefly luciferase kinetics / M. A. Kirillova [et al.] // Int. J. Biol. Macromol. - 2019. - Vol. 131. - P691-696, DOI 10.1016/j.ijbiomac.2019.03.110 . - ISSN 0141-8130
Кл.слова (ненормированные):
Bioluminescence -- Heat shock protein 90 -- High-throughput screening -- adenosine triphosphate -- apyrase -- bovine serum albumin -- firefly luciferase -- heat shock protein 90 -- stabilizing agent -- Article -- bioluminescence -- clinical study -- conformation -- controlled study -- denaturation -- enzyme activity -- enzyme kinetics -- high throughput screening -- incubation time -- nonhuman -- protein protein interaction -- protein refolding -- temperature -- thermal denaturation -- time
Аннотация: The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90 (Hsp90) without additional co-chaperone supplementation; intended for high-throughput screening of anti-cancer drugs and prognosis of stress. In this regard, Hsp90 and adenosine 5?-triphosphate (ATP) mediated firefly luciferase (FLuc) kinetics was investigated using apyrase and FLuc as client proteins. Bioluminescent assay containing Hsp90, ATP, and apyrase led to complete loss of luminescence at 50 °C which indicates the protective role of Hsp90 against thermal denaturation. Similarly, the assay sample comprising Hsp90, ATP, and FLuc showed 2 fold increments in luminescence than their counterparts. Introduction of bovine serum albumin (BSA) to the pre-incubated assay mixture led to an initial rise in the luminescence (28%) in comparison to the sample containing Hsp90, ATP and FLuc. Therefore, FLuc based HTS assays are not suitable for clinical samples which may contain stabilizing agents. However, thermally denatured FLuc and apyrase could not regain their active conformation even when Hsp90 and ATP were introduced in the assay system. This observation justifies the role of Hsp90 to be protective rather than a reparation agent when acts without co-chaperones. © 2019

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Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Kirillova, M. A.; Ranjan, R.; Esimbekova, E. N.; Kratasyuk, V. A.

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Estimating CO2 gas exchange in mixed age vegetable plant communities grown on soil-like substrates for life support systems / V. V. Velichko, A. A. Tikhomirov, S. A. Ushakova // Life Sci. Space Res. - 2018. - Vol. 16. - P47-51, DOI 10.1016/j.lssr.2017.11.001 . - ISSN 2214-5524
Кл.слова (ненормированные):
Bioconversion of plant waste -- CO2 gas exchange -- Conveyor mode -- Plant cultivation -- Soil-like substrate -- carbon dioxide -- Article -- atmosphere -- beet -- carrot -- concentration (parameters) -- Cyperus esculentus -- gas exchange -- genetic variation -- microclimate -- nonhuman -- plant age -- plant community -- plant growth -- planting density -- priority journal -- reproducibility -- soil and soil related phenomena -- soil like substrate -- vegetable
Аннотация: If soil-like substrate (SLS) is to be used in human life support systems with a high degree of mass closure, the rate of its gas exchange as a compartment for mineralization of plant biomass should be understood. The purpose of this study was to compare variations in CO2 gas exchange of vegetable plant communities grown on the soil-like substrate using a number of plant age groups, which determined the so-called conveyor interval. Two experimental plant communities were grown as plant conveyors with different conveyor intervals. The first plant community consisted of conveyors with intervals of 7 days for carrot and beet and 14 days for chufa sedge. The conveyor intervals in the second plant community were 14 days for carrot and beet and 28 days for chufa sedge. This study showed that increasing the number of age groups in the conveyor and, thus, increasing the frequency of adding plant waste to the SLS, decreased the range of variations in CO2 concentration in the “plant–soil-like substrate” system. However, the resultant CO2 gas exchange was shifted towards CO2 release to the atmosphere of the plant community with short conveyor intervals. The duration of the conveyor interval did not significantly affect productivity and mineral composition of plants grown on the SLS. © 2017

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Velichko, V. V.; Tikhomirov, A. A.; Ushakova, S. A.

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Gamma irradiation of resting eggs of Moina macrocopa affects individual and population performance of hatchlings / E. Zadereev [et al.] // J. Environ. Radioact. - 2017. - Vol. 175-176. - P126-134, DOI 10.1016/j.jenvrad.2017.05.002 . - ISSN 0265-931X
Кл.слова (ненормированные):
Cladocera -- Life cycle parameters -- Population performance -- Resting eggs -- ?-radiation -- Gamma rays -- Irradiation -- Life cycle -- Radiation effects -- Radioactive materials -- Sediments -- Cladocera -- Cycle parameters -- Dose response relationships -- Population performance -- Reproductive rates -- Resting eggs -- Sensitive parameter -- Zooplankton communities -- Radiation -- adolescent -- Cladocera -- contamination -- controlled study -- dose response -- female -- gamma irradiation -- gamma radiation -- hatchling -- human -- human experiment -- life cycle -- Moina macrocopa -- newborn -- nonhuman -- sediment -- zooplankton -- Animalia -- Cladocera -- Moina macrocopa
Аннотация: We investigated the effects of ?-radiation on the survival of resting eggs of the cladoceran Moina macrocopa, on the parameters of the life cycle of neonates hatched from the irradiated eggs and on the performance of the population initiated from irradiated eggs. The study showed that ?-radiation in a range of doses from the background level to 100 Gy had no effect on survival of irradiated eggs. The absorbed dose of 200 Gy was lethal to resting eggs of M. macrocopa. The number of clutches and net reproductive rate (R0) of hatchlings from eggs exposed to radiation were the strongly affected parameters in experiments with individual females. The number of clutches per female was drastically reduced for females hatched from egg exposed to 80–100 Gy. The most sensitive parameter was the R0. The estimated ED50 for the R0 (effective dose that induces 50% R0 reduction) was 50 Gy. Population performance was also affected by the irradiation of the resting stage of animals that initiated population. Populations that was initiated from hatchlings from resting eggs exposed to 100 Gy was of smaller size and with fewer juvenile and parthenogenetic females in comparison with control populations. Thus, we determined the dose-response relationship for the effect of gamma radiation on survival of resting eggs and individual and population responses of hatchlings from irradiated resting eggs. We conclude that for highly polluted areas contamination of bottom sediments with radioactive materials could affect zooplankton communities through adverse chronic effects on resting eggs, which will be transmitted to hatchlings at individual or population levels. © 2017 Elsevier Ltd

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Держатели документа:
Institute of Biophysics, Federal Research Centre Krasnoyarsk Scientific Centre, Siberian Branch, Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodniy Ave., Krasnoyarsk, Russian Federation
Budker Institute of Nuclear Physics, 11 Akademika Lavrent'eva Ave., Novosibirsk, Russian Federation

Доп.точки доступа:
Zadereev, E.; Lopatina, T.; Oskina, N.; Zotina, T.; Petrichenkov, M.; Dementyev, D.

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10.

A narrowing of the phenotypic diversity range after large rearrangements of the karyotype in salmonidae: The relationship between saltational genome rearrangements and gradual adaptive evolution / A. A. Makhrov // Genes. - 2017. - Vol. 8, Is. 11, DOI 10.3390/genes8110297 . - ISSN 2073-4425
Кл.слова (ненормированные):
Ecology -- Evolution -- Genome -- Karyotype -- Morphology -- ecology -- gene rearrangement -- genome -- karyotype -- morphology -- nonhuman -- salmonid
Аннотация: The problem of how a gradual development of ecological and morphological adaptations combines with large genome rearrangements, which have been found to occur in the phylogeny of many groups of organisms, is a matter of discussion in the literature. The objective of this work was to study the problem with the example of salmonids, whose evolution included at least six events of multiple chromosome fusions. Large karyotype rearrangements are associated with a decrease in ecological and morphological diversity in salmonids. In the above example, genome rearrangements seem to distort the function of the genetic systems that are responsible for the occurrence of certain ecological forms in salmonids. © 2017 by the authors; Licensee MDPI, Basel, Switzerland.

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Держатели документа:
A.N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, Moscow, Russian Federation
Institute of Biophysics of Siberian Branch of Federal Research Center, “Krasnoyarsk Science Center” of Russian Academy of Sciences, Akademgorodok, 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Makhrov, A. A.

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11.

Low doses of gamma-radiation induce SOS response and increase mutation frequency in Escherichia coli and Salmonella typhimurium cells / A. Bolsunovsky [et al.] // Ecotoxicol. Environ. Saf. - 2016. - Vol. 134. - P233-238, DOI 10.1016/j.ecoenv.2016.09.009 . - ISSN 0147-6513
Кл.слова (ненормированные):
Absorbed dose -- Ames test -- Dose rate -- Mutation rate -- SOS chromotest -- Ames test -- cell death -- DNA repair -- Escherichia coli -- experimental model -- gamma radiation -- limit of detection -- long term exposure -- mutation rate -- nonhuman -- SOS chromotest -- Bacteria (microorganisms) -- Escherichia coli -- Salmonella typhimurium
Аннотация: This study addresses use of two bacterial test systems (the Ames test and the SOS chromotest) to estimate the effects of low doses of γ-radiation. The most substantial increases in induction of SOS response and mutation frequencies were observed in the first 24 h of exposure to γ-radiation as compared to the cells in the exposure-free control. Gamma-radiation also impaired growth and survival of S. typhimurium cells in the first 24 h. The effects were attenuated at lower exposure doses and at longer exposure times. In the experiments conducted in this study, at 96 h of exposure, the values of some of the γ-radiation effects were lower than the MID (minimum inducing dose) detection limits and, thus, were neglected. Long-term exposure to γ-radiation could also result in combined effects of γ-radiation and the death of cells in the culture. © 2016 Elsevier Inc.

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Держатели документа:
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
FRC Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Novosibirsk State University, Novosibirsk, Russian Federation

Доп.точки доступа:
Bolsunovsky, A.; Frolova, T.; Dementyev, D.; Sinitsyna, O.

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12.

Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments / T. A. Zotina [et al.] // Environ. Toxicol. Chem. - 2015. - Vol. 34, Is. 10. - P2310-2321, DOI 10.1002/etc.3057 . - ISSN 0730-7268
Кл.слова (ненормированные):
Aquatic plants -- Biomarkers -- Genotoxicity -- Sediment quality -- Sediment toxicity -- Biomarkers -- Chromosomes -- Cytotoxicity -- Pollution -- Pollution control -- Radioactive waste disposal -- Radioactivity -- River pollution -- Sediments -- Toxicity -- Aquatic plants -- Genotoxicities -- Laboratory bioassay -- Radioactive contamination -- Radioactive pollution -- Sediment quality -- Sediment toxicity -- Toxicity endpoints -- Rivers -- Article -- bioassay -- controlled study -- cytotoxicity -- Elodea canadensis -- environmental exposure -- genotoxicity -- indicator organism -- lake sediment -- mitosis index -- nonhuman -- plant growth -- plant root -- priority journal -- radioactive pollution -- river -- root length -- Russian Federation -- sensitivity analysis -- shoot -- toxicity testing -- Elodea -- Elodea canadensis
Аннотация: The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shoots
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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zotina, T. A.; Trofimova, E. A.; Medvedeva, M. Y.; Dementyev, D. V.; Bolsunovsky, A. Y.

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13.

A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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14.

Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug / A. V. Murueva [et al.] // Journal of Materials Science: Materials in Medicine. - 2013. - Vol. 24, Is. 8. - P1905-1915, DOI 10.1007/s10856-013-4941-2 . - ISSN 0957-4530
Кл.слова (ненормированные):
3-hydroxybutyric acid -- Average diameter -- Cell attachments -- Chemical compositions -- Mass concentration -- Mouse-fibroblasts -- Polyhydroxyalkanoates -- Solvent evaporation techniques -- Biocompatibility -- Cell culture -- Cells -- Loading -- 3 hydroxybutyric acid -- 3 hydroxyhexanoic acid -- 4 hydroxybutyric acid -- 4',6 diamidino 2 phenylindole -- DNA -- doxorubicin -- nanoparticle -- polyhydroxyalkanoic acid -- polymer -- polystyrene -- solvent -- unclassified drug -- animal cell -- article -- biocompatibility -- biodegradability -- cell adhesion -- cell proliferation -- cell strain 3T3 -- cell viability -- chemical composition -- chemical structure -- controlled study -- cytotoxicity -- drug efficacy -- drug release -- electrophoretic mobility -- encapsulation -- evaporation -- fibroblast -- in vitro study -- nonhuman -- particle size -- priority journal -- stain -- study -- surface charge -- zeta potential
Аннотация: Microparticles made from degradable polyhydroxyalkanoates of different chemical compositions a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric and 3-hydroxyvaleric acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoic acids (P3HB/3HHx) were prepared using the solvent evaporation technique, from double emulsions. The study addresses the influence of the chemical compositions on the size and ?-potential of microparticles. P3HB microparticles loaded with doxorubicin have been prepared and investigated. Their average diameter and ?-potential have been found to be dependent upon the level of loading (1, 5, and 10 % of the polymer mass). Investigation of the in vitro drug release behavior showed that the total drug released from the microparticle into the medium increased with mass concentration of the drug. In this study mouse fibroblast NIH 3T3 cells were cultivated on PHA microparticles, and results of using fluorescent DAPI DNA stain, and MTT assay showed that microparticles prepared from PHAs of different chemical compositions did not exhibit cytotoxicity to cells cultured on them and proved to be highly biocompatible. Cell attachment and proliferation on PHA microparticles were similar to those on polystyrene. The cytostatic drug encapsulated in P3HB/3HV microparticles has been proven to be effective against HeLa tumor cells. В© 2013 Springer Science+Business Media New York.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Institute of Modern Biology and Biotechnology, Siberian Federal University, Svobodny Av. 79, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
Engineering Systems Division, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, Cambridge, MA 02139, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Murueva, A.V.; Shishatskaya, E.I.; Kuzmina, A.M.; Volova, T.G.; Sinskey, A.J.

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15.

Study of the efficiency of doxorubicin deposited in microparticles from resorbable bioplastotaneв„ў on laboratory animals with Ehrlich's solid carcinoma / E. I. Shishatskaya, A. V. Goreva, A. M. Kuzmina // Bulletin of Experimental Biology and Medicine. - 2013. - Vol. 154, Is. 6. - P773-777, DOI 10.1007/s10517-013-2053-0 . - ISSN 0007-4888
Кл.слова (ненормированные):
Bioplastotane -- controlled drug delivery systems -- Ehrlich's carcinoma -- microparticles -- resorbable polymers -- doxorubicin -- drug carrier -- animal experiment -- animal model -- animal tissue -- antineoplastic activity -- article -- cancer inhibition -- controlled study -- drug delivery device -- drug delivery system -- drug dosage form comparison -- drug efficacy -- drug mechanism -- Ehrlich ascites tumor -- encapsulation -- leukocyte count -- mouse -- multiple cycle treatment -- nonhuman -- oncological parameters -- tumor volume -- tumor weight -- Animalia -- Mus
Аннотация: Antitumor efficiency of an experimental form of an experimental form of anthracyclin antibiotic (doxorubicin), resorbable microparticles from Bioplastotaneв„ў, was studied on laboratory mice with transplanted Ehrlich's solid carcinoma. Use of the experimental form of the cytostatic in polymeric microparticles from resorbable Bioplastotaneв„ў in animals with solid tumor led to inhibition of the cancerous process, comparable to that in response to intravenous free doxorubicin, but without negative effects on the blood system. В© 2013 Springer Science+Business Media New York.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Institute of Basic Biology and Biotechnology, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Goreva, A.V.; Kuzmina, A.M.

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16.

Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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17.

Stable isotope composition of fatty acids in organisms of different trophic levels in the yenisei river / M. I. Gladyshev [et al.] // PLoS ONE. - 2012. - Vol. 7, Is. 3. - Ст. e34059, DOI 10.1371/journal.pone.0034059 . - ISSN 1932-6203
Кл.слова (ненормированные):
carbon 13 -- fatty acid -- carbon -- Apatania crymophila -- article -- benthos -- Eulimnogammarus viridis -- fatty acid analysis -- fatty acid desaturation -- Fontinalis antipyretica -- food chain -- food web -- freshwater fish -- isotope analysis -- lipid composition -- microalga -- moss -- nonhuman -- pelagic zone -- river ecosystem -- Russian Federation -- stable isotope analysis -- Thymallus arcticus -- trophic level -- animal -- chemistry -- larva -- metabolism -- river -- Bryophyta -- Gammaridae -- Trichoptera -- Animals -- Carbon Isotopes -- Fatty Acids -- Food Chain -- Larva -- Microalgae -- Rivers
Аннотация: We studied four-link food chain, periphytic microalgae and water moss (producers), trichopteran larvae (consumers I), gammarids (omnivorous - consumers II) and Siberian grayling (consumers III) at a littoral site of the Yenisei River on the basis of three years monthly sampling. Analysis of bulk carbon stable isotopes and compound specific isotope analysis of fatty acids (FA) were done. As found, there was a gradual depletion in 13C contents of fatty acids, including essential FA upward the food chain. In all the trophic levels a parabolic dependence of ? 13C values of fatty acids on their degree of unsaturation/chain length occurred, with 18:2n-6 and 18:3n-3 in its lowest point. The pattern in the ? 13C differences between individual fatty acids was quite similar to that reported in literature for marine pelagic food webs. Hypotheses on isotope fractionation were suggested to explain the findings. В© 2012 Gladyshev et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Kalachova, G.S.; Makhutova, O.N.

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18.

Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals / E. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - P2185-2203, DOI 10.1163/092050610X537138 . - ISSN 0920-5063
Кл.слова (ненормированные):
14C products of polymer resorption -- intravenous administration -- microparticles -- Resorbable polyhydroxybutyrate -- tissues of internal organs -- 3-hydroxybutyric acid -- sup14/supC products of polymer resorption -- Adverse effect -- Drug-delivery systems -- Growth and development -- High molecular weight -- Initial molecular weight -- Initial values -- Internal organs -- Intravenous administration -- Laboratory animals -- Local response -- matrix -- Mean diameter -- Micro-particles -- Polymer degradation -- Polymer microparticles -- Preparation process -- Radioactivity level -- Residual content -- Resorbable -- Sustained-release -- Tail veins -- Wistar rat -- Animals -- Biocompatibility -- Degradation -- Histology -- Molecular weight -- Morphology -- Radiation -- Radioactivity -- Rats -- Tissue -- poly(3 hydroxybutyric acid) -- animal experiment -- animal tissue -- article -- biocompatibility -- concentration process -- controlled study -- degradation -- drug delivery system -- female -- gel permeation chromatography -- heart -- kidney parenchyma -- liver -- lung parenchyma -- molecular weight -- nonhuman -- organ culture -- priority journal -- radioactivity -- rat -- spleen -- Animals -- Animals, Laboratory -- Biocompatible Materials -- Drug Delivery Systems -- Female -- Infusions, Intravenous -- Materials Testing -- Microspheres -- Molecular Weight -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Animalia -- Rattus -- Rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.; Goreva, A.; Kalacheva, G.; Volova, T.

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19.

Is the fatty acid composition of freshwater zoobenthic invertebrates controlled by phylogenetic or trophic factors? / O. N. Makhutova [et al.] // Lipids. - 2011. - Vol. 46, Is. 8. - P709-721, DOI 10.1007/s11745-011-3566-9 . - ISSN 0024-4201
Кл.слова (ненормированные):
Benthic invertebrates -- Feeding strategy -- Fish -- Phylogeny -- Polyunsaturated fatty acids -- arachidonic acid -- docosahexaenoic acid -- icosapentaenoic acid -- article -- controlled study -- Dendrocoelopsis -- Eulimnogammarus viridis -- fatty acid analysis -- feeding behavior -- freshwater fish -- habitat -- lipid composition -- nonhuman -- nutritional value -- phylogeny -- species differentiation -- taxonomy -- Animals -- Environment -- Fatty Acids -- Fresh Water -- Humans -- Invertebrates -- Phylogeny -- Ara -- Crustacea -- Dendrocoelopsis -- Eulimnogammarus viridis -- Invertebrata -- Turbellaria
Аннотация: We studied the fatty acid (FA) content and composition of ten zoobenthic species of several taxonomic groups from different freshwater bodies. Special attention was paid to essential polyunsaturated fatty acids, eicosapentaenoic acid (EPA, 20:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and arachidonic acid (ARA, 20:4n-6); and the n-3/n-6 and DHA/ARA ratios, which are important for consumers of higher trophic levels, i.e., fish. The content and ratios of these FA varied significantly in the studied zoobenthic species, consequently, the invertebrates were of different nutritional quality for fish. Eulimnogammarus viridis (Crustacea) and Dendrocoelopsis sp. (Turbellaria) had the highest nutrition value for fish concerning the content of EPA and DHA and n-3/n-6 and DHA/ARA ratios. Using canonical correspondence analysis we compared the FA profiles of species of the studied taxa taking into account their feeding strategies and habitats. We gained evidence that feeding strategy is of importance to determine fatty acid profiles of zoobenthic species. However, the phylogenetic position of the zoobenthic species is also responsible and may result in a similar fatty acid composition even if species or populations inhabit different water bodies or have different feeding strategies. В© 2011 AOCS.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Science, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodny av 79, Krasnoyarsk 660041, Russian Federation
Institute for Biology of Inland Waters of the Russian Academy of Science, Borok 152742, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Makhutova, O.N.; Sushchik, N.N.; Gladyshev, M.I.; Ageev, A.V.; Pryanichnikova, E.G.; Kalachova, G.S.

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20.

Biochemical fractionation and cellular distribution of americium and plutonium in the biomass of freshwater macrophytes / T. A. Zotina, G. S. Kalacheva, A. Y. Bolsunovsky // Journal of Radioanalytical and Nuclear Chemistry. - 2011. - Vol. 290, Is. 2. - P447-451, DOI 10.1007/s10967-011-1228-2 . - ISSN 0236-5731
Кл.слова (ненормированные):
238, 242Pu -- 241Am -- Carbohydrates -- Cellulose -- Fractionation -- Protein -- Submerged macrophyte -- americium 241 -- plutonium -- plutonium 238 -- plutonium 242 -- polysaccharide -- unclassified drug -- article -- bioaccumulation -- biomass -- cellular distribution -- Ceratophyllum demersum -- controlled study -- cytosol -- Elodea canadensis -- Fontinalis antipyretica -- fractionation -- freshwater species -- macrophyte -- moss -- Myriophyllum spicatum -- nonhuman -- plant cell -- radiation absorption -- radioactivity -- shoot
Аннотация: Accumulation of americium ( 241Am) and plutonium ( 238,242Pu) and their distribution in cell compartments and biochemical components of the biomass of freshwater aquatic plants Elodea canadensis, Ceratophyllum demersum and Myrioplyllum spicatum and aquatic moss Fontinalis antipyretica have been investigated in laboratory experiments. Americium and plutonium taken up from water by Elodea canadensis apical shoots were mainly absorbed by structural components of plant cells (90% for 241Am; 89% for 238Pu and 82-87% for 242Pu). About 10-18% of isotope activity was recorded in the cytosol fraction. The major concentration (76-92%) of americium was bound to cell wall cellulose-like polysaccharides of Elodea canadensis, Myriophyllum spicatum, Ceratophyllum demersum and Fontinalis antipyretica, 8-24% of americium activity was registered in the fraction of proteins and carbohydrates, and just a minor concentration (<1%) in the lipid fraction. The distribution of plutonium in the biomass fractions of Elodea was similar to that of americium. Hence, americium and plutonium had the highest affinity to cellulose-like polysaccharides of cell walls of freshwater submerged macrophytes. В© 2011 Akademiai Kiado, Budapest, Hungary.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Kalacheva, G.S.; Bolsunovsky, A.Y.

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