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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitel'son I.I., Tikhomirov A.A., Parshina O.V., Ushakova S.A., Kalacheva G.S.
Заглавие : Volatile metabolites and external CO2 exchange of wheat cenoses under optimal conditions and thermal stress
Место публикации : Applied Biochemistry and Microbiology. - 2002. - Vol. 38, Is. 1. - С. 78-82. - ISSN 00036838 (ISSN) , DOI 10.1023/A:1013212907872
Ключевые слова (''Своб.индексиров.''): carbon dioxide--volatile agent--article--biosynthesis--carbon dioxide transport--chemical composition--concentration (parameters)--controlled study--cultivar--metabolite--nonhuman--photosynthesis--photosynthetically active radiation--plant metabolism--qualitative analysis--quantitative analysis--stress--temperature sensitivity--thermal exposure--thermostability--wheat--rickettsia sp. par--triticum--triticum aestivum
Аннотация: The effects of elevated temperature (35 and 45В°C) on photosynthesis, respiration, and both the qualitative and quantitative compositions of volatile emissions (VE) of wheat (Triticum aestuvum L. cultivar 232) cenoses at light intensities of 70, 150, or 240 W/m2 of photosynthetically available radiation (PAR) were studied. At a PAR of 240 W/m2, the thermal stabilities of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. Elevated temperatures nonuniformly changed the rates and direction of VE syntheses. In this process, the highest increase in VE evolution was observed at 70 W/m2 and 35В°C; the lowest, at 240 W/m2. In addition, the concentrations and composition of VE during the repair period differed from the initial values.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina T. A., Trofimova E. A., Medvedeva M. Y., Dementyev D. V., Bolsunovsky A. Y.
Заглавие : Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments
Место публикации : Environ. Toxicol. Chem. - 2015. - Vol. 34, Is. 10. - С. 2310-2321. - ISSN 07307268 (ISSN) , DOI 10.1002/etc.3057
Ключевые слова (''Своб.индексиров.''): aquatic plants--biomarkers--genotoxicity--sediment quality--sediment toxicity--biomarkers--chromosomes--cytotoxicity--pollution--pollution control--radioactive waste disposal--radioactivity--river pollution--sediments--toxicity--aquatic plants--genotoxicities--laboratory bioassay--radioactive contamination--radioactive pollution--sediment quality--sediment toxicity--toxicity endpoints--rivers--article--bioassay--controlled study--cytotoxicity--elodea canadensis--environmental exposure--genotoxicity--indicator organism--lake sediment--mitosis index--nonhuman--plant growth--plant root--priority journal--radioactive pollution--river--root length--russian federation--sensitivity analysis--shoot--toxicity testing--elodea--elodea canadensis
Аннотация: The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shootslength of shootsmitotic indexlength of rootspercentage of abnormal cells. The response of the genotoxicity endpoint (percentage of cells with chromosome abnormalities in roots of Elodea) was the highest in sediments with chemical pollution, whereas the highest inhibition of toxicity endpoints (shoot and root length) occurred in sediments with the highest level of radioactive pollution. The extreme response of Elodea endpoints to the quality of certain sediment samples may be regarded as related to the possible presence of unknown toxicants. The results show that E. canadensis can be used as an indicator species in laboratory contact testing of bottom sediment. The responses of shoot and root length growth endpoints of Elodea can be recommended as basic sensitivity indicators of bottom sediment toxicity. Analysis of cells carrying abnormal chromosomes in the apical root meristem of Elodea can be performed optionally in the same test to assess the genotoxicity of sediments. © 2015 SETAC.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Место публикации : Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - С. 719-728. - ISSN 09574530 (ISSN) , DOI 10.1023/B:JMSM.0000030215.49991.0d
Ключевые слова (''Своб.индексиров.''): necrosis--polymeric implants--suppurative inflammation--tissue reaction--biodegradation--calcification (biochemistry)--cells--copolymers--implants (surgical)--silk--tissue--tumors--materials science--acid phosphatase--copolymer--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polyhydroxyvaleric acid--unclassified drug--animal experiment--animal model--article--biodegradable implant--blood vessel reactivity--catgut--controlled study--enzyme activity--female--giant cell--histochemistry--inflammation--macrophage--nonhuman--priority journal--rat--silk--suture--tensile strength--tissue reaction--tissue structure--wound healing--young modulus--absorbable implants--animals--female--fibrosis--foreign-body reaction--hydroxybutyrates--muscle, skeletal--polyesters--polymers--rats--rats, wistar--sutures--treatment outcome--wounds, penetrating--animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Efremov S.N., Puzyr' A.P., Mogil'Naya O.A.
Заглавие : Tissue response to biodegradable suture threads made of polyhydroxyalkanoates
Место публикации : Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - С. 210-217. - ISSN 00063398 (ISSN) , DOI 10.1023/A:1021184119268
Ключевые слова (''Своб.индексиров.''): acid phosphatase--alkaline phosphatase--polyhydroxyalkanoic acid--animal experiment--animal tissue--article--biocompatibility--biodegradability--controlled study--elasticity--enzyme activity--enzyme mechanism--female--histochemistry--incision--nonhuman--physical chemistry--postoperative period--rat--rigidity--suture--thickness--tissue reaction--wound healing--animalia
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Tissue reaction to intramuscular injection of resorbable polymer microparticles
Место публикации : Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - С. 786-790. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-007-0432-0
Ключевые слова (''Своб.индексиров.''): microencapsulation--polyhydroxybutyrate--resorbable polymers--tissue reaction--poly(3 hydroxybutyric acid)--polymer--animal experiment--animal tissue--article--cell infiltration--controlled study--drug delivery system--drug formulation--female--giant cell--inflammation--macrophage--microencapsulation--nonhuman--rat--tissue reaction--absorbable implants--animals--drug compounding--female--foreign-body reaction--injections, intramuscular--microspheres--polyesters--rats--rats, wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein
Место публикации : Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - С. 774-779. - ISSN 0006291X (ISSN) , DOI 10.1006/bbrc.1995.1880
Ключевые слова (''Своб.индексиров.''): riboflavin--article--bioluminescence--fluorescence--nonhuman--priority journal--protein analysis--protein synthesis--vibrio--vibrionaceae--bacterial proteins--chromatography, gel--chromatography, thin layer--flavin mononucleotide--flavoproteins--luminescence--riboflavin--spectrometry, fluorescence--support, u.s. gov't, p.h.s.--vibrio--bacteria (microorganisms)--photobacterium--vibrio--vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova M. D., Markova S. V., Tikunova N. V., Vysotski E. S.
Заглавие : The smallest isoform of Metridia longa luciferase as a fusion partner for hybrid proteins
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2020. - Vol. 21, Is. 14. - Ст.4971. - С. 1-16. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21144971
Аннотация: Bioluminescent proteins are widely used as reporter molecules in various in vitro and in vivo assays. The smallest isoform of Metridia luciferase (MLuc7) is a highly active, naturally secreted enzyme which, along with other luciferase isoforms, is responsible for the bright bioluminescence of marine copepod Metridia longa. In this study, we report the construction of two variants of a hybrid protein consisting of MLuc7 and 14D5a single-chain antibody to the surface glycoprotein E of tick-borne encephalitis virus as a model fusion partner. We demonstrate that, whereas fusion of a single-chain antibody to either N-or C-terminus of MLuc7 does not affect its bioluminescence properties, the binding site on the single-chain antibody influences its binding capacity. The affinity of 14D5a-MLuc7 hybrid protein (KD = 36.2 nM) where the C-terminus of the single-chain antibody was fused to the N-terminus of MLuc7, appeared to be 2.5-fold higher than that of the reverse, MLuc7-14D5a (KD = 87.6 nM). The detection limit of 14D5a-MLuc7 hybrid protein was estimated to be 45 pg of the recombinant glycoprotein E. Although the smallest isoform of M. longa luciferase was tested as a fusion partner only with a single-chain antibody, it is reasonable to suppose that MLuc7 can also be successfully used as a partner for genetic fusion with other proteins. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Furyaeva A.V., Shevchenko V.T., Pechurkin N.S.
Заглавие : The effect of temperature on the growth kinetics of Candida tropicalis 29-10 continuously cultivated in the pH-stat at different growth-limiting conditions
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1986. - Vol. 22, Is. 2. - С. 243-247. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): candida tropicalis--continuous culture--fungus--growth--nonhuman--ph--temperature
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Makurina V.I., Kuznetsov A.M., Kudryasheva N.S., Plotnikova N.B., Medvedeva S.E., Gritsenko I.S., Chernykh V.P.
Заглавие : The effect of succinic acid sulfoderivatives on bacterial luminescence
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1991. - Vol. 27, Is. 1. - С. 127-133. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--n (arylsulfonamido)succinimide--unclassified drug--article--bacterium--bioluminescence--nonhuman--bacteria (microorganisms)
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorban A.N., Mirkes E.M., Popova T.G., Sadovsky M.G.
Заглавие : The comparative redundancy of genes of various organisms and viruses
Место публикации : Genetika. - 1993. - Vol. 29, Is. 9. - P1413-1419. - ISSN 00166758 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--dna content--evolution--genome--nonhuman--restriction mapping--virus--amino acid sequence--comparative study--gene frequency--human--molecular genetics--nucleotide sequence--restriction mapping--virus gene--amino acid sequence--base sequence--comparative study--english abstract--gene frequency--genes, viral--human--molecular sequence data--restriction mapping
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S., Bondar' V.S.
Заглавие : Study of the luminescence system of the soil enchytraeid Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady Biochemistry and Biophysics. - 2003. - Vol. 391, Is. 1-6. - С. 204-207. - ISSN 16076729 (ISSN) , DOI 10.1023/A:1025105323648
Ключевые слова (''Своб.индексиров.''): adsorption chromatography--animal cell--annelid worm--article--luminescence--nonhuman--purification--animal--drug antagonism--isolation and purification--metabolism--physiology--soil--animalia--annelida--clitellata--enchytraeidae--edetic acid--luciferase--magnesium--animals--edetic acid--luciferases--luminescent measurements--magnesium--oligochaeta--soil
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chang D., Liu Y., Chen Y., Hu X., Burov A., Puzyr A., Bondar V., Yao L.
Заглавие : Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system
Место публикации : BMC Vet. Res.: BioMed Central Ltd., 2020. - Vol. 16, Is. 1. - Ст.202. - ISSN 17466148 (ISSN), DOI 10.1186/s12917-020-02422-3
Аннотация: Background: Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results: The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions: Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection. © 2020 The Author(s).
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Goreva A.V., Kuzmina A.M.
Заглавие : Study of the efficiency of doxorubicin deposited in microparticles from resorbable bioplastotaneв„ў on laboratory animals with Ehrlich's solid carcinoma
Место публикации : Bulletin of Experimental Biology and Medicine. - 2013. - Vol. 154, Is. 6. - С. 773-777. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-013-2053-0
Ключевые слова (''Своб.индексиров.''): bioplastotane--controlled drug delivery systems--ehrlich's carcinoma--microparticles--resorbable polymers--doxorubicin--drug carrier--animal experiment--animal model--animal tissue--antineoplastic activity--article--cancer inhibition--controlled study--drug delivery device--drug delivery system--drug dosage form comparison--drug efficacy--drug mechanism--ehrlich ascites tumor--encapsulation--leukocyte count--mouse--multiple cycle treatment--nonhuman--oncological parameters--tumor volume--tumor weight--animalia--mus
Аннотация: Antitumor efficiency of an experimental form of an experimental form of anthracyclin antibiotic (doxorubicin), resorbable microparticles from Bioplastotaneв„ў, was studied on laboratory mice with transplanted Ehrlich's solid carcinoma. Use of the experimental form of the cytostatic in polymeric microparticles from resorbable Bioplastotaneв„ў in animals with solid tumor led to inhibition of the cancerous process, comparable to that in response to intravenous free doxorubicin, but without negative effects on the blood system. В© 2013 Springer Science+Business Media New York.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Popova T.G.
Заглавие : Study of biological properties of polyhydroxyalkanoates in a long-term experiment in vivo
Место публикации : Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - С. 218-222. - ISSN 00063398 (ISSN) , DOI 10.1023/A:1021136203338
Ключевые слова (''Своб.индексиров.''): hemoglobin--polyhydroxyalkanoic acid--animal experiment--animal tissue--article--biocompatibility--biodegradability--biosynthesis--blood cell count--blood sampling--comparative study--controlled study--dna synthesis--elasticity--enzyme activity--enzyme mechanism--erythrocyte sedimentation rate--female--in vivo study--liver function--nonhuman--organ weight--rat--suture--animalia
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing
Место публикации : Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 3. - С. 249-252. - ISSN 00036838 (ISSN) , DOI 10.1023/B:ABIM.0000025946.47013.03
Ключевые слова (''Своб.индексиров.''): alkanoic acid--carbon monoxide--coal--hydrogen--lignite--algorithm--article--bacterial strain--bacterium culture--coal gasification--controlled study--gas--gas exchange--mathematical computing--nonhuman--ralstonia eutropha--bacteria (microorganisms)--ralstonia--wautersia eutropha
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Kalachova G.S., Makhutova O.N.
Заглавие : Stable isotope composition of fatty acids in organisms of different trophic levels in the yenisei river
Место публикации : PLoS ONE. - 2012. - Vol. 7, Is. 3. - Ст.e34059. - ISSN 19326203 (ISSN) , DOI 10.1371/journal.pone.0034059
Ключевые слова (''Своб.индексиров.''): carbon 13--fatty acid--carbon--apatania crymophila--article--benthos--eulimnogammarus viridis--fatty acid analysis--fatty acid desaturation--fontinalis antipyretica--food chain--food web--freshwater fish--isotope analysis--lipid composition--microalga--moss--nonhuman--pelagic zone--river ecosystem--russian federation--stable isotope analysis--thymallus arcticus--trophic level--animal--chemistry--larva--metabolism--river--bryophyta--gammaridae--trichoptera--animals--carbon isotopes--fatty acids--food chain--larva--microalgae--rivers
Аннотация: We studied four-link food chain, periphytic microalgae and water moss (producers), trichopteran larvae (consumers I), gammarids (omnivorous - consumers II) and Siberian grayling (consumers III) at a littoral site of the Yenisei River on the basis of three years monthly sampling. Analysis of bulk carbon stable isotopes and compound specific isotope analysis of fatty acids (FA) were done. As found, there was a gradual depletion in 13C contents of fatty acids, including essential FA upward the food chain. In all the trophic levels a parabolic dependence of ? 13C values of fatty acids on their degree of unsaturation/chain length occurred, with 18:2n-6 and 18:3n-3 in its lowest point. The pattern in the ? 13C differences between individual fatty acids was quite similar to that reported in literature for marine pelagic food webs. Hypotheses on isotope fractionation were suggested to explain the findings. В© 2012 Gladyshev et al.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Zolotukhin I.G., Tikhomirov A.A., Tikhomirova N.A., Kudenko Yu.A., Gribovskaya I.V., Balnokin Yu., Gros J.B.
Заглавие : Some methods for human liquid and solid waste utilization in bioregenerative life-support systems
Место публикации : Applied Biochemistry and Biotechnology. - 2008. - Vol. 151, Is. 2-3. - С. 676-685. - ISSN 02732289 (ISSN) , DOI 10.1007/s12010-008-8291-3
Ключевые слова (''Своб.индексиров.''): closure--human waste--life-support systems--salicornia--sodium chloride--above-ground biomass--biological lives--bioregenerative--closure--cultivation process--culture methods--human waste--irrigation waters--life-support systems--manned space missions--mineral elements--physico-chemical methods--salicornia--salicornia europaea--salt-tolerant--soil-like substrates--biomass--body fluids--electrodialysis--grain (agricultural product)--irrigation--liquids--metal refining--minerals--mining--oxidation--plant shutdowns--sodium chloride--soils--solid wastes--substrates--water supply--vegetation--article--biomass--controlled study--electrodialysis--halophyte--irrigation (agriculture)--microclimate--nonhuman--recycling--salicornia europaea--solid waste--bioremediation--dialysis--feces--goosefoot--growth, development and aging--human--methodology--salt tolerance--urine--waste management--wheat--batis maritima--salicornia--salicornia europaea--triticum aestivum--biodegradation, environmental--chenopodiaceae--dialysis--feces--humans--life support systems--salt-tolerance--triticum--urine--waste management
Аннотация: Bioregenerative life-support systems (BLSS) are studied for developing the technology for a future biological life-support system for long-term manned space missions. Ways to utilize human liquid and solid wastes to increase the closure degree of BLSS were investigated. First, urine and faeces underwent oxidation by Kudenko's physicochemical method. The products were then used for root nutrition of wheat grown by the soil-like substrate culture method. Two means of eliminating sodium chloride, introduced into the irrigation solution together with the products of urine oxidation, were investigated. The first was based on routine electrodialysis of irrigation water at the end of wheat vegetation. Dialysis eliminated about 50% of Na from the solution. This desalinization was performed for nine vegetations. The second method was new: after wheat cultivation, the irrigation solution and the solution obtained by washing the substrate containing mineral elements not absorbed by the plants were used to grow salt-tolerant Salicornia europaea L. plants (saltwort). The above-ground biomass of this plant can be used as a food, and roots can be added to the soil-like substrate. Four consecutive wheat and Salicornia vegetations were cultivated. As a result of this wheat and Salicornia cultivation process, the soil-like substrate salinization by NaCl were considerably decreased. В© 2008 Humana Press.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wu Y. -T., Chiang P. -W., Tandon K., Rogozin D. Y., Degermendzhy A. G., Tang S. -L.
Заглавие : Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia
Место публикации : Microb. Genomics: Microbiology Society, 2021. - Vol. 7, Is. 12. - Ст.000712. - ISSN 20575858 (ISSN), DOI 10.1099/mgen.0.000712
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kirillova M. A., Ranjan R., Esimbekova E. N., Kratasyuk V. A.
Заглавие : Role of Hsp90 and ATP in modulating apyrase activity and firefly luciferase kinetics
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 691-696. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.110
Ключевые слова (''Своб.индексиров.''): bioluminescence--heat shock protein 90--high-throughput screening--adenosine triphosphate--apyrase--bovine serum albumin--firefly luciferase--heat shock protein 90--stabilizing agent--article--bioluminescence--clinical study--conformation--controlled study--denaturation--enzyme activity--enzyme kinetics--high throughput screening--incubation time--nonhuman--protein protein interaction--protein refolding--temperature--thermal denaturation--time
Аннотация: The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90 (Hsp90) without additional co-chaperone supplementation; intended for high-throughput screening of anti-cancer drugs and prognosis of stress. In this regard, Hsp90 and adenosine 5?-triphosphate (ATP) mediated firefly luciferase (FLuc) kinetics was investigated using apyrase and FLuc as client proteins. Bioluminescent assay containing Hsp90, ATP, and apyrase led to complete loss of luminescence at 50 °C which indicates the protective role of Hsp90 against thermal denaturation. Similarly, the assay sample comprising Hsp90, ATP, and FLuc showed 2 fold increments in luminescence than their counterparts. Introduction of bovine serum albumin (BSA) to the pre-incubated assay mixture led to an initial rise in the luminescence (28%) in comparison to the sample containing Hsp90, ATP and FLuc. Therefore, FLuc based HTS assays are not suitable for clinical samples which may contain stabilizing agents. However, thermally denatured FLuc and apyrase could not regain their active conformation even when Hsp90 and ATP were introduced in the assay system. This observation justifies the role of Hsp90 to be protective rather than a reparation agent when acts without co-chaperones. © 2019
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20.

Вид документа : Статья из журнала
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Автор(ы) : Volova T., Shishatskaya E., Sevastianov V., Efremov S., Mogilnaya O.
Заглавие : Results of biomedical investigations of PHB and PHB/PHV fibers
Место публикации : Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - С. 125-133. - ISSN 1369703X (ISSN) , DOI 10.1016/S1369-703X(03)00038-X
Ключевые слова (''Своб.индексиров.''): biocompatibility--fibers--implants--poly-3-hydroxybutyrate (phb)--poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (phb/phv)--polyhydroxyalkanoates (phas)--tissue reaction--bacteriology--calcification (biochemistry)--copolymers--flammability--tissue--tissue response--biomedical engineering--acid phosphatase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid)--polyhydroxyalkanoic acid--suture material--unclassified drug--animal experiment--article--bacterial metabolism--biochemistry--biocompatibility--biomedicine--calcification--catgut--correlation analysis--dose time effect relation--enzyme activity--fascia--fiber--foreign body--functional assessment--giant cell--implantation--in vivo study--inflammation--intermethod comparison--macrophage--materials testing--multinuclear cell--muscle injury--necrosis--nonhuman--parameter--phagocytosis--physiology--postoperative period--priority journal--process monitoring--ralstonia eutropha b 5786--scar formation--silk--strength--suture--synthesis--tissue reaction--wautersia eutropha--wound healing--animalia--ralstonia--wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.
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