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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodionova N.S., Petushkov V.N.
Заглавие : Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
Место публикации : Journal of Photochemistry and Photobiology B: Biology. - 2006. - Vol. 83, Is. 2. - С. 123-128. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2005.12.014
Ключевые слова (''Своб.индексиров.''): atp--bioluminescence--earthworms--ions--luciferin-luciferase systems--triton x-100--adenosine triphosphate--anion--bromine--calcium ion--carbonic acid--cation--chloride--chromium derivative--detergent--dodecyl sulfate sodium--inorganic salt--iodine--iron derivative--luciferase--luciferin--magnesium ion--manganese--nitrate--phosphate--sulfate--sulfite--triton x 100--annelid worm--article--bioluminescence--concentration (parameters)--controlled study--enzyme activation--enzyme activity--enzyme inhibition--enzyme mechanism--in vitro study--nonhuman--priority journal--qualitative analysis--quantitative analysis--adenosine triphosphate--animals--cations, divalent--cations, monovalent--detergents--firefly luciferin--kinetics--luciferases--luminescence--metals--oligochaeta--photobiology--salts--annelida--clitellata--earthworms (sp.)--enchytraeidae--fridericia heliota--oligochaeta (metazoa)--pheretima sieboldi
Аннотация: The study addresses the effect produced by different inorganic salts and detergents (SDS, Triton X-100, the Tween series) on the ATP-dependent bioluminescent reaction catalyzed by the luciferase of the new earthworm species Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae). It has been shown that the effect of divalent metal salts on luminescence is determined by the action of cations. Three of them - Mg2+, Mn2+ and Ca2+ - can stimulate luciferase activity at concentrations varying within a wide range, and Mn2+ can act as a 100%-effective substitute for Mg2+ in F. heliota luminescence reaction in vitro. The inhibitory effect of monovalent metal salts on luminescence is largely determined by the action of the anion part of the molecule. The effectiveness of the inhibitory effect of anions increases in the following order: {Mathematical expression}. Of the sodium salts, dodecyl sulfate, which is an anionic detergent, produces the strongest inhibitory effect on luciferase. On the contrary, nonionic detergents produce a stimulatory effect on the F. heliota luciferase. The action of the most effective of them - Triton X-100 - is determined by its ability to reduce the actual concentration of lipid inhibitors in the reaction mixture. В© 2006 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S.
Заглавие : New types of luminescent systems of soil enchytraeids (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady Biochemistry and Biophysics. - 2005. - Vol. 401, Is. 1-6. - С. 115-118. - ISSN 16076729 (ISSN) , DOI 10.1007/s10628-005-0047-1
Ключевые слова (''Своб.индексиров.''): annelid worm--article--energy transfer--epidermis cell--luminescence--nonhuman--soil analysis--animals--luciferases--luminescence--oligochaeta--annelida--clitellata--enchytraeidae
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Marques S.M., Petushkov V.N., Rodionova N.S., Esteves Da Silva J.C.G.
Заглавие : LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota
Место публикации : Journal of Photochemistry and Photobiology B: Biology. - 2011. - Vol. 102, Is. 3. - С. 218-223. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2010.12.006
Ключевые слова (''Своб.индексиров.''): bioluminescence--earthworm--fridericia heliota--luciferin--microscale nmr--rp-hplc-ms--alkene--alkyl group--benzothiazole--carboxylic acid--hydroxyl group--luciferin--pterin--absorption--article--bioluminescence--earthworm--fridericia heliota--isomer--molecular weight--nonhuman--nuclear magnetic resonance--priority journal--reversed phase high performance liquid chromatography--ultraviolet radiation--animals--chromatography, high pressure liquid--chromatography, reverse-phase--firefly luciferin--luminescent agents--magnetic resonance spectroscopy--mass spectrometry--oligochaeta--fridericia heliota
Аннотация: This paper presents the main results of RP-HPLC-MS and microscale NMR analysis performed on Accompanying similar to Luciferin (AsLn(x)), compounds present in extracts of the bioluminescent earthworm Fridericia heliota that display similarities with Fridericia's luciferin, the substrate of the bioluminescent reaction. Three isomers of AsLn were discovered, AsLn(1), AsLn(2) and AsLn(3), all of which present a molecular weight of 529 Da. Their UV-Vis absorption spectra show maxima at 235 nm for AsLn(1), 238 and 295 nm for AsLn(2) and 241 and 295 nm for AsLn(3). MS n fragmentation patterns suggest the existence of carboxylic acid and hydroxyl moieties, and possibly chemical groups found in other luciferins like pterin or benzothiazole. The major isomer, AsLn(2), presents an aromatic ring and alkene and alkyl moieties. These luciferin-like compounds can be used as models that could give further insights into the structure of this newly discovered luciferin. В© 2010 Elsevier Inc. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S.
Заглавие : Purification and partial spectral characterization of a novel luciferin from the luminous enchytraeid Fridericia heliota
Место публикации : Journal of Photochemistry and Photobiology B: Biology. - 2007. - Vol. 87, Is. 2. - С. 130-136. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2007.03.006
Ключевые слова (''Своб.индексиров.''): atp--bioluminescence--earthworms--enchytraeid--luciferase--luciferin--adenosine triphosphate--luciferase--luciferin--animal cell--anion exchange chromatography--annelid worm--article--controlled study--earthworm--firefly--luminance--luminescence--nonhuman--priority journal--protein analysis--protein purification--radiation absorption--reversed phase liquid chromatography--ultraviolet radiation--adenosine triphosphate--animals--luciferases--luminescent agents--oligochaeta--spectrum analysis--enchytraeidae--fridericia heliota
Аннотация: A homogeneous luciferin preparation has been obtained from the luminous soil enchytraeid Fridericia heliota, which has an ATP-dependent luminescent system. A procedure for luciferin purification without losing fractions of active luciferase has been developed. The luciferin specific activity is 4000 times increased; its UV absorption spectrum maximum is 294 nm with a local minimum at 262 nm. The luciferin of the enchytraeid F. heliota is significantly different from firefly luciferin, whose luminescent reaction also requires ATP, and it also appears to have no similarities to other known luciferins. В© 2007 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodionova N.S., Bondar' V.S., Petushkov V.N.
Заглавие : ATP is a cosubstrate of the luciferase of the earthworm Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady Biochemistry and Biophysics. - 2003. - Vol. 392, Is. 1-6. - С. 253-255. - ISSN 16076729 (ISSN) , DOI 10.1023/A:1026134628735
Ключевые слова (''Своб.индексиров.''): adenosine diphosphate--adenosine phosphate--adenosine triphosphate--luciferase--luciferin--magnesium--animal cell--article--controlled study--earthworm--hydrolysis--luminescence--nonhuman--adenosine diphosphate--adenosine triphosphate--animals--firefly luciferin--kinetics--luciferases--luminescent measurements--magnesium--oligochaeta--substrate specificity--animalia--annelida--clitellata--enchytraeidae--pheretima sieboldi
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein
Место публикации : Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - С. 774-779. - ISSN 0006291X (ISSN) , DOI 10.1006/bbrc.1995.1880
Ключевые слова (''Своб.индексиров.''): riboflavin--article--bioluminescence--fluorescence--nonhuman--priority journal--protein analysis--protein synthesis--vibrio--vibrionaceae--bacterial proteins--chromatography, gel--chromatography, thin layer--flavin mononucleotide--flavoproteins--luminescence--riboflavin--spectrometry, fluorescence--support, u.s. gov't, p.h.s.--vibrio--bacteria (microorganisms)--photobacterium--vibrio--vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Lee J.
Заглавие : Purification and characterization of flavoproteins and cytochromes from the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1
Место публикации : European Journal of Biochemistry. - 1997. - Vol. 245, Is. 3. - С. 790-796. - ISSN 00142956 (ISSN)
Ключевые слова (''Своб.индексиров.''): anisotropy--lumazine protein--photobacterium--thioredoxin reductase--time-resolved fluorescence--cytochrome--flavoprotein--article--bioluminescence--nonhuman--priority journal--protein analysis--protein purification--sea--vibrio--amino acid sequence--bacterial proteins--cytochromes--flavoproteins--molecular sequence data--sequence alignment--vibrio--azotobacter--bacteria (microorganisms)--escherichia coli--haemophilus--haemophilus influenza--murinae--negibacteria--photobacterium--photobacterium leiognathi--pseudomonas--uncultured marine bacterium--vibrio fischeri
Аннотация: Several flavoproteins and cytochromes that occur as major components in extracts of the yellow bioluminescence Y1 strain of the murine bacterium Vibrio fischeri have been purified and characterized with respect to their mass (SDS/PAGE) and matrix-assisted laser-desorption/ionization MS), chromatographic properties, N-terminal sequence, and spectroscopy (absorption, fluorescence emission and anisotropy decay). The investigated proteins were as follows: yellow fluorescence protein (YFP) with bound riboflavin, FMN or 6,7-dimethyl-8-ribityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflavin, or 6- methyl-7-oxo-ribityllumazine; thioredoxin reductase with FAD as ligand; and two c-type diheme cytochromes, c551 and c554. We present evidence that the riboflavin-bound YFP has an N-terminal sequence corresponding to that published for the dimeric YFP. We show that an equilibrium replacement of the riboflavin can be made with excess lumazine derivative and that lumazine- bound YFP has different bioluminescence properties to those of the lumazine protein from Photobacterium leiognathi. BFP is a different protein again, and in the bacterial lysate it occurs in multiple forms, ligated to either riboflavin, lumazine, or t he 7-oxolumazine derivative. The N-terminal sequence for BFP-shows similarities to those of the YFP proteins and to lumazine protein and riboflavin synthase from Photobacterium. BFP in any form has no bioluminescence or riboflavin-synthase activity. A 70-kDa fluorescent flavoprotein with FAD as ligand has an N-terminal sequence highly similar to those of thioredoxin reductases from Haemophilus influenza and Escherichia coli. Cytochrome contaminations in previous preparations of YFP have been removed and an identified as the two c-type cytochromes c551 and c554. Both inhibit the NADH-induced bioluminescence in the reductase/luciferase system with the luciferase from P. leiognathi and V. fischeri. The N-terminal amino acid sequence of the cytochrome (c551) corresponds to a diheme cytochrome c4. The spectral properties of c554 are similar to those of other c5 cytochromes, and both c554 and c551 have absorption spectra similar to those of the respective cytochromes from the gram-negative bacteria Pseudomonas and Azotobacter.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S., Bondar' V.S.
Заглавие : Study of the luminescence system of the soil enchytraeid Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady Biochemistry and Biophysics. - 2003. - Vol. 391, Is. 1-6. - С. 204-207. - ISSN 16076729 (ISSN) , DOI 10.1023/A:1025105323648
Ключевые слова (''Своб.индексиров.''): adsorption chromatography--animal cell--annelid worm--article--luminescence--nonhuman--purification--animal--drug antagonism--isolation and purification--metabolism--physiology--soil--animalia--annelida--clitellata--enchytraeidae--edetic acid--luciferase--magnesium--animals--edetic acid--luciferases--luminescent measurements--magnesium--oligochaeta--soil
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates
Место публикации : Biochemistry. - 1995. - Vol. 34, Is. 10. - С. 3300-3309. - ISSN 00062960 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--recombinant protein--article--ligand binding--nonhuman--priority journal--protein isolation--protein protein interaction--protein stability--vibrionaceae--bacterial proteins--binding sites--carrier proteins--circular dichroism--flavin mononucleotide--fluorescence polarization--genes, bacterial--kinetics--ligands--luciferase--luminescence--molecular sequence data--photobacterium--recombinant proteins--spectrophotometry--support, u.s. gov't, p.h.s.--photobacterium leiognathi--vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotsky E.S., Zavoruev V.V., Mezhevikin V.V.
Заглавие : NADPH- and ATP-dependent luminescence of extracts from luminous bacteria
Место публикации : Biokhimiya. - 1982. - Vol. 47, Is. 12. - С. 1983-1987. - ISSN 03209725 (ISSN)
Ключевые слова (''Своб.индексиров.''): adenosine triphosphate--reduced nicotinamide adenine dinucleotide phosphate--bacterium--bioluminescence--nonhuman
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Stasishina G.N., Kasayeva G.E.
Заглавие : Growth of hydrogen-oxidizing bacteria and carboxydobacteria studied during their combined cultivation
Место публикации : Mikrobiologiya. - 1984. - Vol. 53, Is. 5. - С. 709-713. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon monoxide--hydrogen--bacterial growth--nonhuman
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : terskov I.A., Sid'ko A.F., Sid'ko F.Y., Vasil'ev V.A.
Заглавие : Estimate of the content of chlorophyll a of phytoplankton in the surface layer of meso- and eutrophic waters by the optical remote-sensing method
Место публикации : Doklady Biophysics. - 1989. - Vol. 304-306. - С. 77-79. - ISSN 00124974 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--article--microorganism--nonhuman--phytoplankton
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nedorezov L.V., Khlebopros R.G.
Заглавие : Peculiarities of dynamics of population numbers in strongly inertial and without inertial regulation
Место публикации : ZH. OBSHCH. BIOL. - 1985. - Vol. 46, Is. 3. - С. 345-348
Ключевые слова (''Своб.индексиров.''): entomophage--mathematical model--nonbiological model--nonhuman--phytophage
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Gribovskaya I.V.
Заглавие : A growth medium for the carboxydobacterium Seliberia carboxydohydrogena
Место публикации : Mikrobiologiya. - 1986. - Vol. 55, Is. 1. - С. 72-76. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon monoxide--bacterial growth--culture medium--nonhuman
Аннотация: The growth of the carboxydobacterium Seliberia carboxydohydrogena Z-1062 was studied at a different concentration of mineral elements under the conditions of continuous turbidostat cultivation. The chemical composition of the bacterium was determined and the dynamics of its specific growth rate and the intracellular content of mineral elements were studied when the concentration of these elements was changed in the growth medium. The synthesis of 1 g of biomass by this strain required: N, 120 mg; P, 23 mg; S, 5.5 mg; K, 1.5 mg; Mg, 3.5 mg. Changes in the residual concentrations of the elements within a broad range had no effect on the specific rate of the carboxydobacterial growth and chemical composition.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Goreva A.V., Kuzmina A.M.
Заглавие : Study of the efficiency of doxorubicin deposited in microparticles from resorbable bioplastotaneв„ў on laboratory animals with Ehrlich's solid carcinoma
Место публикации : Bulletin of Experimental Biology and Medicine. - 2013. - Vol. 154, Is. 6. - С. 773-777. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-013-2053-0
Ключевые слова (''Своб.индексиров.''): bioplastotane--controlled drug delivery systems--ehrlich's carcinoma--microparticles--resorbable polymers--doxorubicin--drug carrier--animal experiment--animal model--animal tissue--antineoplastic activity--article--cancer inhibition--controlled study--drug delivery device--drug delivery system--drug dosage form comparison--drug efficacy--drug mechanism--ehrlich ascites tumor--encapsulation--leukocyte count--mouse--multiple cycle treatment--nonhuman--oncological parameters--tumor volume--tumor weight--animalia--mus
Аннотация: Antitumor efficiency of an experimental form of an experimental form of anthracyclin antibiotic (doxorubicin), resorbable microparticles from Bioplastotaneв„ў, was studied on laboratory mice with transplanted Ehrlich's solid carcinoma. Use of the experimental form of the cytostatic in polymeric microparticles from resorbable Bioplastotaneв„ў in animals with solid tumor led to inhibition of the cancerous process, comparable to that in response to intravenous free doxorubicin, but without negative effects on the blood system. В© 2013 Springer Science+Business Media New York.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Vysotsky E.S.
Заглавие : Changes of lipids and their fatty acid composition during batch cultivation of the luminescent bacterium
Место публикации : Mikrobiologiya. - 1984. - Vol. 53, Is. 6. - С. 932-937. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--lipid--bacterium--bioluminescence--nonhuman
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Salnikov M.V., Vysotsky E.S., Zavoruyev V.V., Mezhevikin V.V.
Заглавие : Changes in the periplasmic space of luminescent bacteria depending on the intensity of luminescence
Место публикации : Mikrobiologiya. - 1984. - Vol. 53, Is. 5. - С. 744-747. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--bacterium--bioluminescence--electron microscopy--nonhuman
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Terskov I.A., Gitelzon I.I., Sidko Ya. F.
Заглавие : Biological and nutritional value of the hydrogen bacteria biomass
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1984. - Vol. 20, Is. 4. - С. 540-550. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): alcaligenes eutrophus--diet--methodology--nonhuman--nutritional value
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Vysotsky E.S., Zavoruev V.V., Mezhevikin V.V., Raibekas A.A.
Заглавие : Obtaining bacterial luciferase for bioluminescent analysis
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1988. - Vol. 24, Is. 6. - С. 745-753. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--enzyme isolation--enzyme subunit structure--molecular weight--nonhuman--photobacterium leiognathi
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Nikolaeva E. D., Kirichenko A. K., Shishatskaya E. I.
Заглавие : Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 230-240. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.068
Примечания : Cited By :1
Ключевые слова (''Своб.индексиров.''): bacterial cellulose--composites--polyhydroxyalkanoates--3 hydroxybutyric acid 4 hydroxybutyric acid copolymer--actovegin--bacterial cellulose--cellulose--collagen type 1--copolymer--cytokeratin 10--cytokeratin 14--solcoseryl--unclassified drug--angiogenesis--animal cell--animal experiment--animal model--animal tissue--biochemical analysis--biocompatibility--burn--cell growth--controlled study--cutaneous parameters--differential scanning calorimetry--drug release--elemental analysis--female--fibroblast--histopathology--in vitro study--in vivo study--inflammation--mechanics--nonhuman--rat--review--wound healing--wound planimetry--x ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019
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