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A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

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A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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A QUANTUM CHEMICAL STUDY OF THE FORMATION OF 2-HYDROPEROXY-COELENTERAZINE IN THE Ca2+-REGULATED PHOTOPROTEIN OBELIN [Text] / L. Y. Antipina [et al.] // J. Struct. Chem. - 2011. - Vol. 52, Is. 5. - P870-875. - Cited References: 19. - The work was supported by RFBR (07-04-00930-a), the "Molecular and Cell Biology" Program of the Presidium of the Russian Academy of Sciences, and the Program of the Siberian Division of the Russian Academy of Sciences (project No. 2) within the implementation of the Federal Targeted Program "Scientific and Scientific Pedagogical Personnel of Innovative Russia, 2010" (P333 and P213). . - ISSN 0022-4766

РУБ Chemistry, Inorganic & Nuclear + Chemistry, Physical
Рубрики:
CALCIUM-DISCHARGED OBELIN
   SEMIEMPIRICAL METHODS
   1.7 ANGSTROM
   OPTIMIZATION
   PARAMETERS
   MECHANISM
   FLUORESCENCE
   ELEMENTS
   PROTEIN
   EMITTER
Кл.слова (ненормированные):
coelenterazine -- 2-hydroperoxy-coelenterazine -- Obelia longissima -- Renilla muelleri
Аннотация: The Ca2+-regulated photoprotein obelin determines the luminescence of the marine hydroid Obelia longissima. Bioluminescence is initiated by calcium and appears as a result of the oxidative decarboxylation related to the coelenterazine substrate. The luciferase of the luminescent marine coral Renilla muelleri (RM) also uses coelenterazine as a substrate. However, three proteins are involved in the in vivo bioluminescence of these animals: luciferase, green fluorescent protein, and Ca2+-regulated coelenterazine-binding protein (CBP). In fact, CBP that contains one strongly bound coelenterazine molecule is the RM luciferase substrate in the in vivo bioluminescent reaction. Coelenterazine becomes available for oxygen and the reaction with luciferase only after binding CBP with calcium ions. Unlike Ca2+-regulated photoproteins, the coelenterazine molecule is not activated by oxygen in the CBP molecule. In this work, by means of quantum chemical methods the behavior of substrates in these proteins is analyzed. It is shown that coelenterazine can form different tautomers: CLZ(2H) and CLZ(7H). The formation of 2-hydroperoxy-coelenterazine is studied. According to the obtained data, these proteins use different forms of the substrates for the reaction. In obelin, the substrate is in the CLZ(2H) form that affords hydrogen peroxide. In RM, coelenterazine is in the CLZ(7H) form, and therefore, CBP is not activated by oxygen.

Держатели документа:
[Antipina, L. Yu
Tomilin, F. N.
Ovchinnikov, S. G.] Russian Acad Sci, LV Kirensky Phys Inst, Siberian Div, Krasnoyarsk, Russia
[Vysotskii, E. S.] Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia
[Antipina, L. Yu
Ovchinnikov, S. G.] MF Reshetnev Siberian State Aerosp Univ, Krasnoyarsk, Russia
ИФ СО РАН
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Antipina, L.Y.; Tomilin, F.N.; Vysotskii, E.S.; Ovchinnikov, S.G.

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An optimization model of competition between species for resource. / V. G. Sukhovol'skii, R. G. Khlebopros, T. R. Iskhakov // Doklady. Biochemistry and biophysics. - 2003. - Vol. 390. - P171-173 . - ISSN 1607-6729
Кл.слова (ненормированные):
animal -- article -- biological model -- comparative study -- competitive behavior -- computer simulation -- ecosystem -- evaluation -- growth, development and aging -- insect -- physiology -- plankton -- population dynamics -- population growth -- quality control -- species difference -- validation study -- Animals -- Competitive Behavior -- Computer Simulation -- Ecosystem -- Insects -- Models, Biological -- Plankton -- Population Dynamics -- Population Growth -- Quality Control -- Species Specificity

Scopus
Держатели документа:
Sukachev Institute of Forest, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sukhovol'skii, V.G.; Khlebopros, R.G.; Iskhakov, T.R.

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An optimization model of the photosynthetic leaf: the model of optimal photosynthetic CO2 fixation within leaves of mesophytic C3 plants. / V. G. Soukhovolsky [et al.] // Doklady Biological Sciences. - 2002. - Vol. 382. - P28-30 . - ISSN 0012-4966
Кл.слова (ненормированные):
carbon dioxide -- ribulosebisphosphate carboxylase -- article -- biological model -- ecosystem -- light -- metabolism -- photosynthesis -- plant leaf -- radiation exposure -- Carbon Dioxide -- Ecosystem -- Light -- Models, Biological -- Photosynthesis -- Plant Leaves -- Ribulose-Bisphosphate Carboxylase

Scopus
Держатели документа:
Institute of Forest, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Soukhovolsky, V.G.; Fomina, I.R.; Bil, K.; Nishio, J.N.; Khlebopros, R.G.

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Model approaches to description of critical phenomena in forest ecosystems / A. S. Isaev, V. G. Soukhovolsky, R. G. Khlebopros // Contemporary Problems of Ecology. - 2011. - Vol. 4, Is. 7. - P699-705, DOI 10.1134/S1995425511070010 . - ISSN 1995-4255
Кл.слова (ненормированные):
critical phenomena -- ecosystem -- modeling -- phase transitions -- population -- population dynamics -- data set -- ecosystem modeling -- forest ecosystem -- forest fire -- insect -- optimization -- population dynamics -- succession -- Hexapoda
Аннотация: Methodological bases of optimization modeling approach are considered in regard to describing critical phenomena in forest ecosystems. Models of insects' outbreaks, forest successions, forest fires as second order phase transitions are proposed. It is shown that there is fair correlation between model calculations and observations data. В© 2011 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Center on the Problems of Ecology and Productivity of Forests, Russian Academy of Sciences, ul. Profsoyuznaya 84/32, Moscow 117997, Russian Federation
Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk 660036, Russian Federation
Intern Cntr 4 Rrch o Extr. St. of Organi w in Pres., of Krasnoyarsk Res Ctr of Sib Brnch of the Russ. Ac of Sc, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Isaev, A.S.; Soukhovolsky, V.G.; Khlebopros, R.G.

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Planar bandpass filter with 100-dB suppression up to Tenfold passband frequency [] / B. A. Belyaev [et al.] // Prog. Electromagn. Res. C. - 2014. - Vol. 48. - P37-44 . - ISSN 1937-8718
Кл.слова (ненормированные):
Fractional bandwidths -- Microwave bandpass filter -- Optimization rules -- Passband frequencies -- Resonator filters -- Stripline resonators -- Substrate surface -- Suspended substrates -- Microwave filters -- Substrates
Аннотация: This paper proposes a miniature microwave bandpass filter. It comprises quasi-lumped suspended substrate stripline resonators of a new type. Their common substrate consists of two contiguous dielectric layers. Every resonator in the filter has three parallel strip conductors. Two of them are placed on the outer substrate surfaces and the rest is placed inside the substrate. The filter of the sixth order was designed with the use of special optimization rules that are universal for all resonator filters. The substrate of the fabricated filter was made of RT/Duroid 5880 (?r = 2.2, ? = 0.002) with thickness of 0.127mm. It has dimensions of 12 mm ? 45.5 mm. The measured passband has a center frequency of 1.01 GHz, 3-dB fractional bandwidth of 11%, and minimum insertion loss of 1.4dB. The 100-dB upper stopband extends up to 10.5 GHz.

Scopus
Держатели документа:
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Institute of Engineering Physics and Radio Electronics, Siberian Federal University, Krasnoyarsk 660074, Russian Federation
Reshetnev Siberian State Aerospace University, Krasnoyarsk 660014, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Belyaev, B.A.; Serzhantov, A.M.; Tyurnev, V.V.; Bal'Va, Y.F.; Leksikov, A.A.

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Production and properties of bacterial cellulose by the strain Komagataeibacter xylinus B-12068 / T. G. Volova [et al.] // Appl. Microbiol. Biotechnol. - 2018. - P1-12, DOI 10.1007/s00253-018-9198-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
Bacterial cellulose -- Growth conditions -- Komagataeibacter xylinus -- Biocompatibility -- Cell culture -- Cellulose -- Cultivation -- Glucose -- 3t3 mouse fibroblasts -- Bacterial cellulose -- Cultivation conditions -- Emission spectrometry -- Ethanol concentrations -- Growth conditions -- Komagataeibacter xylinus -- Physical and mechanical properties -- Substrates
Аннотация: A strain of acetic acid bacteria, Komagataeibacter xylinus B-12068, was studied as a source for bacterial cellulose (BC) production. The effects of cultivation conditions (carbon sources, temperature, and pH) on BC production and properties were studied in surface and submerged cultures. Glucose was found to be the best substrate for BC production among the sugars tested; ethanol concentration of 3% (w/v) enhanced the productivity of BC. Optimization of medium and cultivation conditions ensures a high production of BC on glucose and glycerol, up to 2.4 and 3.3 g/L/day, respectively. C/N elemental analysis, emission spectrometry, SEM, DTA, and X-ray were used to investigate the structure and physical and mechanical properties of the BC produced under different conditions. MTT assay and SEM showed that native cellulose membrane did not cause cytotoxicity upon direct contact with NIH 3T3 mouse fibroblast cells and was highly biocompatible. © 2018 Springer-Verlag GmbH Germany, part of Springer Nature

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Держатели документа:
Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Siberian Federal University, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Prudnikova, S. V.; Sukovatyi, A. G.; Shishatskaya, E. I.

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Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass / T. G. Volova, E. G. Kiselev, A. V. Demidenko [et al.] // Polymers. - 2022. - Vol. 14, Is. 1. - Ст. 132, DOI 10.3390/polym14010132. - Cited References:93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties). . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
GLUCOSE-UTILIZING STRAIN
   RALSTONIA-EUTROPHA
   ASPERGILLUS-NIGER
   ACID
Кл.слова (ненормированные):
Jerusalem artichoke hydrolysates -- PHA synthesis -- productivity -- polyhydroxyalkanoates
Аннотация: One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains-C. necator B-10646 and R. eutropha B 5786 and B 8562-were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 degrees C followed by acid hydrolysis at 60 degrees C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100-120 degrees C difference between the T-melt and T-degr, prevailing of the crystalline phase over the amorphous one (C-x between 69 and 75%), and variations in weight average molecular weight (409-480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and epsilon-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology-finding widely available and inexpensive substrates.

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Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Basic Dept Biotechnol, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Phys Inst, Fed Res Ctr, SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana G.; Kiselev, Evgeniy G.; Demidenko, Alexey V.; Zhila, Natalia O.; Nemtsev, Ivan V.; Lukyanenko, Anna V.; Kiselev, Evgeniy; Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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10.

Theory and software of ecological-economical optimization of river water use [Text] / N. N. Degermendzhy, L. G. Kosolapova ; ed. AA Aldama [et al.] // COMPUTATIONAL METHODS IN WATER RESOURCES XI, VOL 2: COMPUTATIONAL METHODS IN SURFACE FLOW AND TRANSPORT PROBLEMS : COMPUTATIONAL MECHANICS PUBLICATIONS LTD, 1996. - 11th International Conference on Computational Methods in Water Resources (CMWR 96) (JUL, 1996, CANCUN, MEXICO). - P. 453-460. - Cited References: 0 . - ISBN 1-85312-487-7

РУБ Mathematics, Applied + Water Resources

Кл.слова (ненормированные):
mathematical model -- preparation of water -- self-purification -- optimization

WOS
Держатели документа:
RAS,INST BIOPHYS SB,ACAD GORODOK,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, N.N.; Kosolapova, L.G.; Aldama, AA \ed.\; Aparicio, J \ed.\; Brebbia, CA \ed.\; Gray, WG \ed.\; Herrera, I \ed.\; Herrer, I \ed.\

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