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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PETUSHKOV V.N., KRATASYUK G.A., RODIONOVA N.S., FISH A.M., BELOBROV P.I.
Заглавие : 2-ENZYME NADH-FMN-OXIDOREDUCTASE-LUCIFERASE SYSTEM FROM LUMINESCENT BACTERIA
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1984. - Vol. 49, Is. 4. - С. 593-603. - 11. - ISSN 0006-2979
Примечания : Cited References: 24
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Egorova O.I., Esimbekova E.N., Kudryashova N.S., Orlova N.Y., L'vova L.S.
Заглавие : A biological luciferase test for the bioluminescent assay of wheat grain infection with Fusarium
Колич.характеристики :3 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 1998. - Vol. 34, Is. 6. - P622-624. - ISSN 0003-6838
Примечания : Cited References: 7
Аннотация: The extent of inhibition of the bioluminescence reaction by wheat grain extracts was studied as a function of the scabby kernel content in wheat. The NADH : flavine mononucleotide oxidoreductase-luciferase bienzyme bioluminescence system was found to be the most sensitive to mycotoxins produced by fungi of the genus Fusarium. A biological luciferase test was developed for monitoring wheat grain infection with Fusarium.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KRATASYUK V.A., ABAKUMOVA V.V., KIM N.B.
Заглавие : A GEL MODEL FOR THE FUNCTIONING OF LUCIFERASE IN THE CELL
Колич.характеристики :5 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1994. - Vol. 59, Is. 7. - P761-765. - ISSN 0006-2979
Примечания : Cited References: 11
Предметные рубрики: BIOLUMINESCENT
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferase--nadh, fmn-oxidoreductase--immobilization
Аннотация: A gel model for the functioning of luciferase in cells has been constructed using bacterial NADH:FMN-oxidoreductase and luciferase immobilized in starch gel disks. The characteristics of the immobilized luciferase depend on the duration of drying, the amount and concentration of the gel, the nature of the support used for drying, and the properties of the initial enzyme preparation. Functionally important enzyme groups remain intact in the immobilized preparation, and luciferase retains its high specificity with respect to aldehydes. The gel microenvironment appears to be optimal for luciferase, judging from its high activity and increased stability. Conditions allowing repeated use of the preparation have been found. The approach permits co-immobilization of luciferase with other enzymes and their substrates. The error in bioluminescence measurements using the disks is 5-10%. A procedure for stabilization of the immobilized luciferase during repeated use has been devised.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk, Valentina A., Stepanova, Lyudmila, V, Ranjan, Rajeev, Sutormin, Oleg S., Pande, Shubhra, Zhukova, Galina, V, Miller, Olga M., Maznyak, Natalya, V, Kolenchukova, Oksana A.
Заглавие : A noninvasive and qualitative bioluminescent assay for express diagnostics of athletes' responses to physical exertion
Колич.характеристики :7 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; Krasnoyarsk Regional Foundation of Science [KF-537]
Место публикации : Luminescence: WILEY, 2020. - Article in press. - ISSN 1522-7235, DOI 10.1002/bio.3954. - ISSN 1522-7243(eISSN)
Примечания : Cited References:33. - The Ministry of Science and Higher Education of the Russian Federation, Grant/Award Number: FSRZ-2020-0006; Krasnoyarsk Regional Foundation of Science, Grant/Award Number: KF-537
Предметные рубрики: SALIVARY BIOMARKERS
EXERCISE
Аннотация: Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence-based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc-Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc-Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I-exp, mean maximum bioluminescence intensity of the experimental measurement (I-exp); I-c, luminescence intensity in control; I-exp/I-c, %) values for participants in the wrestler group while a decrease in the student group (P 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lukyanenko, Kirill A., Belousov, Kirill I., Denisov, Ivan A., Yakimov, Anton S., Esimbekova, Elena N., Bukatin, Anton S., Evstrapov, Anatoly A., Belobrov, Peter I.
Заглавие : Active mixing of immobilised enzymatic system in microfluidic chip
Колич.характеристики :5 с
Коллективы : Russian Science Foundation [15-19-10041]
Место публикации : Micro Nano Lett.: INST ENGINEERING TECHNOLOGY-IET, 2017. - Vol. 12, Is. 6. - С. 377-381. - ISSN 1750-0443, DOI 10.1049/mnl.2016.0646
Примечания : Cited References:17. - The research was supported by the grant of the Russian Science Foundation (project no. 15-19-10041).
Предметные рубрики: POLY(METHYL METHACRYLATE)
SURFACE MODIFICATION
POINT
DEVICES
PMMA
Аннотация: Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to achieve higher sensitivity of bioluminescent reaction. Results of numerical simulations were verified experimentally. The active mixer for dried reagents was made from an electro-mechanical speaker's membrane which was connected to the input of the chip. Such a mixer provided better efficiency than a passive mixing, and it is simple enough for use in point-of-care devices with any systems based on immobilised enzymes in chips.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lukyanenko K. A., Denisov I. A., Yakimov A. S., Esimbekova E. N., Belousov K. I., Bukatin A. S., Kukhtevich I. V., Sorokin V. V., Evstrapov A. A., Belobrov P. I.
Заглавие : Analytical Enzymatic Reactions in Microfluidic Chips
Колич.характеристики :6 с
Коллективы : Russian Science Foundation [15-19-10041]
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2017. - Vol. 53, Is. 7. - С. 775-780. - ISSN 0003-6838, DOI 10.1134/S0003683817070043. - ISSN 1573-8183(eISSN)
Примечания : Cited References:15. - The study was supported by a grant from the Russian Science Foundation (project No. 15-19-10041).
Предметные рубрики: BIOAVAILABLE HEAVY-METALS
DEVICES
POINT
LAB
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferase--microfluidics--microfluidic chip--enzymatic--bioassay
Аннотация: A number of approaches have been proposed and tested to transfer enzymatic reactions into the functional elements of microfluidic chips on the example of the bienzyme bioluminescent reaction involving NAD(P)H:FMN-oxidoreductase and luciferase. Measurement of the catalytic activity of these enzymes (under the influence of pollutants) is the basis of enzymatic bioassay of various liquids. It was found that all of the components of the reaction must be placed in the same cell of the chip to improve the reproducibility of the measurements. The use of starch gel as a carrier for immobilization and gelatin as a scaffold in the reactor of the chip enables the preservation of enzyme activity in the course of sealing the chip at room temperature. It is shown that the components of the reaction should be vigorously stirred in a microfluidic chip reactor to improve the efficiency of the analysis. As a result of the studies, a prototype of microfluidic chip based on the enzymatic bioluminescent reaction is proposed. It is characterized by a detection limit of copper sulfate of 3 mu M that corresponds to the sensitivity of traditional lux-biosensors based on living cells. The analysis time is reduced to 1 min, and the analysis can be performed by individuals without special laboratory skills.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tarasova A.S., Stom D.I., Kudryasheva N.S.
Заглавие : Antioxidant activity of humic substances via bioluminescent monitoring in vitro
Колич.характеристики :8 с
Коллективы : Russian Foundation for Basic Research [15-03-06786a], Russian Academy of Sciences [VI 57.1.1]
Место публикации : Environ. Monit. Assess.: SPRINGER, 2015. - Vol. 187, Is. 3. - Ст.89. - ISSN 0167-6369, DOI 10.1007/s10661-015-4304-1. - ISSN 1573-2959(eISSN)
Примечания : Cited References:51. - This work was supported by the Russian Foundation for Basic Research, Grant No. 15-03-06786a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1.
Предметные рубрики: DETOXIFICATION PROCESSES
TOXICITY
BIOASSAYS
BACTERIA
ASSAY
Ключевые слова (''Своб.индексиров.''): antioxidant activity--oxidative toxicity--general toxicity--humic--substances--bioassay--bioluminescence
Аннотация: This work considers antioxidant properties of natural detoxifying agents-humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature-complex salt K-3[Fe(CN)(6)] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P) H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters-bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients D-GT and D-OxT, respectively. Dependencies of D-GT and D-OxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were 20-min incubation time and 0.5x10(-4) to 2x10(-4) M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Esimbekova, Elena, Kratasyuk, Valentina, Shimomura, Osamu
Заглавие : Application of Enzyme Bioluminescence in Ecology
Колич.характеристики :43 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 67-109. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_3
Примечания : Cited References:85
Предметные рубрики: BACTERIAL LUCIFERASE
IN-VITRO
PYRETHROID INSECTICIDES
FRESH-WATER
Ключевые слова (''Своб.индексиров.''): bioluminescence--ecological monitoring--enzymatic assay--immobilization--integral water toxicity--luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V. A., Esimbekova E. N.
Заглавие : Applications of luminous bacteria enzymes in toxicology
Место публикации : Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - С. 952-959. - ISSN 13862073 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioluminescent toxicity enzymatic assay--immobilization of enzymes--luciferase--total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure. © 2015 Bentham Science Publishers.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk, Valentina A., Esimbekova, Elena N.
Заглавие : Applications of Luminous Bacteria Enzymes in Toxicology
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [15-19-10041]
Место публикации : Comb. Chem. High Throughput Screen: BENTHAM SCIENCE PUBL LTD, 2015. - Vol. 18, Is. 10. - С. 952-959. - ISSN 1386-2073, DOI 10.2174/1386207318666150917100257. - ISSN 1875-5402(eISSN)
Примечания : Cited References:88. - The research was supported by the Russian Science Foundation, project No. 15-19-10041.
Предметные рубрики: NADHFMN-OXIDOREDUCTASE-LUCIFERASE
HUMIC SUBSTANCES
BIOLUMINESCENT
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioluminescent toxicity enzymatic assay--immobilization--of enzymes--luciferase--total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH: FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Место публикации : Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - С. 675-678. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-002-0941-8
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyric acid--acetoacetyl coenzyme a reductase--acetyl coenzyme a acyltransferase--beta hydroxyvalerate--butyrate dehydrogenase--carbon monoxide--electrolyte--hydrogen--oxidoreductase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid)synthase--polyhydroxyalkanoic acid--polymer--unclassified drug--valeric acid--bacterium--article--autotrophy--bacterial growth--bacterial strain--biomass production--controlled study--crystallization--enzyme activity--molecular weight--nonhuman--synthesis--temperature--wautersia eutropha--carbon monoxide--culture media--cupriavidus necator--fatty acids--lipids--polyesters--bacteria (microorganisms)--negibacteria--ralstonia--wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N..., Vetrova E..., Kuznetsov A..., Kratasyuk V..., Stom D...
Заглавие : Bioluminescence assays: Effects of quinones and phenols
Колич.характеристики :5 с
Место публикации : Ecotox. Environ. Safe.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2002. - Vol. 53, Is. 2. - P221-225. - ISSN 0147-6513, DOI 10.1006/eesa.2002.2214
Примечания : Cited References: 18
Ключевые слова (''Своб.индексиров.''): bioluminescence assays--quinones--phenols
Аннотация: The influence of a series of quinones and phenols on bacterial bioluminescence systems was investigated. Three bioluminescence systems used in ecological monitoring were compared: (1) water-soluble; (2) immobilized in starch gel coupled enzyme systems: NADH:FMN-oxidoreductase-luciferase; (3) luminescent bacteria. Bioluminescence inhibition constants of quinones and phenols and bioluminescence induction periods were compared. These kinetic parameters are proportional to quinone concentrations and depend on the quinone redox potential. Different effects of the substances are related to structure and properties of the bioluminescence systems. The set of bioluminescence assays for quinones and phenols monitoring should include two bioluminescence systems: 1 (or 2) and 3. (C) 2002 Elsevier Science (USA).
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova E. N., Nemtseva E. V., Kirillova M. A., Asanova A. A., Kratasyuk V. A.
Заглавие : Bioluminescent assay for toxicological assessment of nanomaterials
Колич.характеристики :4 с
Коллективы : Russian Science Foundation [16-14-10115]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2017. - Vol. 472, Is. 1. - С. 60-63. - ISSN 1607-6729, DOI 10.1134/S1607672917010173. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15. - We are sincerely grateful to the staff of the Institute of Physiological Active Compounds (Kharkiv, Ukraine) for providing fullerene samples. This study was supported by the Russian Science Foundation (project no. 16-14-10115).
Предметные рубрики: LUMINOUS BACTERIA
TOXICITY
Аннотация: A new method for assessing biotoxicity of nanomaterials, based on the use of soluble bioluminescent coupled enzyme system NAD(P)ai...H:FMN oxidoreductase and luciferase, is proposed. The results of this study indicate a significant adverse biological effect exerted by nanoparticles at the molecular level. It was found that the most toxic nanoparticles the nanoparticles are based on copper and copper oxide, as well as single-walled carbon nanotubes and multi-walled carbon nanofibers, which are referred to hazard class II.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva, Nadezhda S., Kovel, Ekaterina S., Sachkova, Anna S., Vorobeva, Anna A., Isakova, Viktoriya G., Churilov, Grigoriy N.
Заглавие : Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds
Колич.характеристики :5 с
Коллективы : Russian Foundation for Basic Research [15-03-06786, 15-43-04377-sibir]; Russian Academy of Sciences [01201351504]
Место публикации : Photochem. Photobiol.: WILEY, 2017. - Vol. 93, Is. 2. - С. 536-540. - ISSN 0031-8655, DOI 10.1111/php.12639. - ISSN 1751-1097(eISSN)
Примечания : Cited References:40. - The work was supported by the Russian Foundation for Basic Research, Grants 15-03-06786 and 15-43-04377-sibir; the state budget allocated to the fundamental research at the Russian Academy of Sciences (project 01201351504).
Предметные рубрики: LUMINOUS MARINE-BACTERIA
HUMIC SUBSTANCES
DETOXIFICATION PROCESSES
Аннотация: A bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH:FMN-oxidoreductase was developed to monitor toxicity and antioxidant activity of bioactive compounds. The assay enables studying toxic effects at the level of biomolecules and physicochemical processes, as well as determining the toxicity of general and oxidative types. Toxic and detoxifying effects of bioactive compounds were studied. Fullerenols, perspective pharmaceutical agents, nanosized particles, water-soluble polyhydroxylated fullerene-60 derivatives were chosen as bioactive compounds. Two homologous fullerenols with different number and type of substituents, C60O2-4(OH)(20-24) and Fe0.5C60(OH) O-x(y) (x + y = 40-42), were used. They suppressed bioluminescent intensity at concentrations 0.01 g L-1 and 0.001 g L-1 for C60O2-4(OH)(20-24) and Fe0.5C60(OH)(x)O-y, respectively; hence, a lower toxicity of C60O2-4(OH)(20-24) was demonstrated. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic oxidizers; changes in toxicities of general and oxidative type were determined; detoxification coefficients were calculated. Fullerenol C60O2-4(OH)(20-24) revealed higher antioxidant ability at concentrations 10(-17)-10(-5) g L-1. The difference in the toxicity and antioxidant activity of fullerenols was explained through their electron donor/acceptor properties and different catalytic activity. Principles of bioluminescent enzyme assay application for evaluating the toxic effect and antioxidant activity of bioactive compounds were summarized and the procedure steps were described.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolosova, Elizaveta M., Sutormin, Oleg S., Stepanova L. V., Shpedt, Aleksandr A., Rimatskaya N. V., Sukovataya, Irina E., Kratasyuk, Valentina A.
Заглавие : Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils
Колич.характеристики :9 с
Коллективы : Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia; Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [18-47-240005]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Environ. Technol. Innov.: ELSEVIER, 2021. - Vol. 24. - Ст.101842. - ISSN 2352-1864, DOI 10.1016/j.eti.2021.101842
Примечания : Cited References:46. - This work was supported by the Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia, and Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [grant number 18-47-240005] in the field of statistical analysis and interpretation of the data; and the work related to the sample collection was supported by the Ministry of Science and Higher Education of the Russian Federation [grant number FSRZ-2020-0006].
Предметные рубрики: FLUORIDE
BIOASSAYS
POLLUTION
METALS
WATER
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. (C) 2021 Elsevier B.V. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolosova E. M., Sutormin O. S., Stepanova L. V., Shpedt A. A., Rimatskaya N. V., Sukovataya I. E., Kratasyuk V. A.
Заглавие : Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils
Место публикации : Environ. Technol. Innov.: Elsevier B.V., 2021. - Vol. 24. - Ст.101842. - ISSN 23521864 (ISSN), DOI 10.1016/j.eti.2021.101842
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. © 2021 Elsevier B.V.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova, Elena N., Nemtseva, Elena V., Bezrukikh, Anna E., Jukova, Galina V., Lisitsa, Albert E., Lonshakova-Mukina, Viktoriya I., Rimatskaya, Nadezhda V., Sutormin, Oleg S., Kratasyuk, Valentina A.
Заглавие : Bioluminescent enzyme inhibition-based assay to predict the potential toxicity of carbon nanomaterials
Колич.характеристики :6 с
Коллективы : Russian Science Foundation [16-14-10115]
Место публикации : Toxicol. Vitro: PERGAMON-ELSEVIER SCIENCE LTD, 2017. - Vol. 45. - С. 128-133. - ISSN 0887-2333, DOI 10.1016/j.tiv.2017.08.022
Примечания : Cited References:55. - This study was supported by the Russian Science Foundation (project no. 16-14-10115).
Предметные рубрики: IN-VIVO
ENGINEERED NANOPARTICLES
NANOTUBE TOXICITY
C-60
FULLERENE
Ключевые слова (''Своб.индексиров.''): nanotoxicity--enzyme inhibition-based assay--bioluminescence--luciferase--nanomaterials--nanotubes
Аннотация: A bioluminescent enzyme inhibition-based assay was applied to predict the potential toxicity of carbon nanomaterials (CNM) presented by single- and multi-walled nanotubes (SWCNT and MWCNT) and aqueous solutions of hydrated fullerene C-60 (C(60)HyFn). This assay specifically detects the influence of substances on parameters of the soluble or immobilised coupled enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc). A protocol based on the optical properties of CNM for correcting the results of the bioluminescent assay was also developed. It was shown that the inhibitory activity of CNM on Red + Luc decreased in the following order: MWCNT SWCNT C(60)HyFn. The soluble enzyme system Red + Luc had high sensitivity to MWCNT and SWCNT, with values of the inhibition parameter IC50 equal to 0.012 and 0.16 mg/L, respectively. The immobilised enzyme system was more vulnerable to C(60)HyFn than its soluble form, with an IC50 equal to 1.4 mg/L. Due to its technical simplicity, rapid response time and high sensitivity, this bioluminescent method has the potential to be developed as a general enzyme inhibition-based assay for a wide variety of nanomaterials.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Vetrova E.V., Kudryasheva N.S.
Заглавие : Bioluminescent water quality monitoring of salt lake Shira
Колич.характеристики :3 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P193-195. - ISSN 1522-7235, DOI 10.1002/(SICI)1522-7243(199907/08)14:4193::AID-BIO5283.3.CO;2-J
Примечания : Cited References: 9
Ключевые слова (''Своб.индексиров.''): bioluminescence--biotest--ecological monitoring--salt lake
Аннотация: The coupled bioluminescent enzyme system luciferase-NADH:FMN-oxidoreductase was used as a biotest in ecological monitoring of the health resort salt lake Shira (South Siberia, Russia). The technique was adapted to saltwater conditions. Bioluminescence kinetic parameters sensitive to pollutants were determined. Conditions for the use of bacterial bioluminescence biotests in salty environmental media were established. Copyright (C) 1999 John Wiley & Sons, Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vetrova E.V., Kudryasheva N.S., Visser AJWG, van Hoek A...
Заглавие : Characteristics of enclogenous flavin fluorescence of Photobacterium leiognathi luciferase and Vibrio fischeri NAD(P)H : FMN-oxidoreductase
Колич.характеристики :5 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2005. - Vol. 20: 11th International Symposium on Luminescence Spectrometry - Detection Techniques in Biomedical and Environmental Analysis (MAY 05-08, 2004, Beijing, JAPAN), Is. 3. - P205-209. - ISSN 1522-7235, DOI 10.1002/bio.815
Примечания : Cited References: 22
Предметные рубрики: FLAVODOXIN
ANISOTROPY
REDUCTASE
DYNAMICS
SYSTEM
Ключевые слова (''Своб.индексиров.''): bacterial bioluminescence--flavin fluorescence
Аннотация: The bioluminescent bacterial enzyme system NAD(P)H:FMN-oxidoreductase-luciferase has been used as a test system for ecological monitoring. One of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. The use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from Photobacterium leiognathi and NAD(P)H:FMN-oxidoreductase from Vibrio fischeri has been proposed. Fluorescence spectroscopy methods have been used to study characteristics of endogenous flavin fluorescence (fluorophore lifetime, the rotational correlation time). The fluorescence anisotropy behaviour of FMN has been analysed and compared to that of the enzyme-bound flavin. The fluorescence characteristics of endogenous flavin of luciferase and NAD(P)H:FMN-oxidoreductase have been shown to be applicable in studying enzymes' interactions with non-fluorescent compounds. Copyright (c) 2005 John Wiley & Sons, Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova E. N., Kalyabina V. P., Kopylova K. V., Torgashina I. G., Kratasyuk V. A.
Заглавие : Design of bioluminescent biosensors for assessing contamination of complex matrices
Место публикации : Talanta: Elsevier B.V., 2021. - Vol. 233. - Ст.122509. - ISSN 00399140 (ISSN), DOI 10.1016/j.talanta.2021.122509
Примечания : Cited By :1
Аннотация: The presence of potentially toxic xenobiotics in complex matrices has become rather the rule than the exception. Therefore, there is a need for highly sensitive inexpensive techniques for analyzing environmental and food matrices for toxicants. Enzymes are selectively sensitive to various toxic compounds, and, thus, they can be used as the basis for detection of contaminants in complex matrices. There are, however, a number of difficulties associated with the analysis of complex matrices using enzyme assays, including the necessity to take into account properties and effects of the natural components of the test media for accurate interpretation of results. The present study describes the six-stage procedure for designing new enzyme sensors intended for assessing the quality of complex matrices. This procedure should be followed both to achieve the highest possible sensitivity of the biosensor to potentially toxic substances and to minimize the effect of the uncontaminated components of complex mixtures on the activity of the biosensor. The proposed strategy has been tested in designing a bioluminescent biosensor for integrated rapid assessment of the safety of fruits and vegetables. The biosensor is based on the coupled enzyme system NAD(P)H:FMN-oxidoreductase and luciferase as the biorecognition element. The study describes methods and techniques for attaining the desired result in each stage. The proposed six-stage procedure for designing bioluminescent enzyme biosensors can be used to design the enzymatic biosensors based on other enzymes. © 2021 Elsevier B.V.
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