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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PETUSHKOV V.N., KRATASYUK G.A., RODIONOVA N.S., FISH A.M., BELOBROV P.I.
Заглавие : 2-ENZYME NADH-FMN-OXIDOREDUCTASE-LUCIFERASE SYSTEM FROM LUMINESCENT BACTERIA
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1984. - Vol. 49, Is. 4. - С. 593-603. - 11. - ISSN 0006-2979
Примечания : Cited References: 24
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PETUSHKOV V.N., RODIONOVA N.S., BELOBROV P.I.
Заглавие : EFFICIENCY OF THE FUNCTIONING OF THE BIENZYMATIC SYSTEM NADH-FMN OXIDOREDUCTASE LUCIFERASE OF LUMINESCENT BACTERIA
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1985. - Vol. 50, Is. 3. - С. 338-342. - 5. - ISSN 0006-2979
Примечания : Cited References: 13
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Петушков В.Н., Родионова Н.С., Белобров П.И.
Заглавие : Изучение эффективности работы биферментной системы NADH: FMN-оксидоредуктаза-люцифераза светящихся бактерий : научное издание
Место публикации : Биохимия. - 1985. - Т. 50, N 3. - С. 401-405. - ISSN 0320-9725
ГРНТИ : 31.27.17
Предметные рубрики: НАДН:ФМН-ОКСИДОРЕДУКТАЗА-ЛЮЦИФЕРАЗА
МЕХАНИЗМ РАБОТЫ
БАКТЕРИИ
СВЕТЯЩИЕСЯ
NADH:FMN-OXIDOREDUCTASE-LUCIFERASE
LUMINESCENT BACTERIA
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PETUSHKOV V.N., RODIONOVA N.S., BELOBROV P.I.
Заглавие : EFFICIENCY OF THE FUNCTIONING OF THE BIENZYMATIC SYSTEM NADH-FMN OXIDOREDUCTASE LUCIFERASE OF LUMINESCENT BACTERIA
Колич.характеристики :5 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1985. - Vol. 50, Is. 3. - P338-342. - ISSN 0006-2979
Примечания : Cited References: 13
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodionova N.S., Petushkov V.N., Belobrov P.I.
Заглавие : Kinetic features of switching of bacterial luciferase from one aldehyde substrate to another
Место публикации : Biophysics. - 1988. - Vol. 33, Is. 3. - С. 424-430. - ISSN 00063509 (ISSN)
Аннотация: In luciferase isolated from luminescing bacteria Vibrio harveyi the authors have studied the dynamics of the luminescence with aliphatic aldehydes C10, C12 and C14 taken in pairs in the reaction with photoreduced flavin mononucleotide (FMN) and in the conjugated system NAD В· H: :FMN-oxidoreductase-luciferase. The kinetic characteristics of endogenous aldehyde have been determined. It is shown that the process of switching of luciferase from one aldehyde substrate to another is dependent on chain length and the order of introducing the aldehydes into the reaction mixture. Analysis of the "matrix of successive perturbations" gave a numerical matrix of the probabilities of oxidation of the aldehydes in the luminescent reaction. An order of preference of the aldehydes on their binding to luciferase is constructed. В© 1989.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KRATASYUK V.A., ABAKUMOVA V.V., KIM N.B.
Заглавие : A GEL MODEL FOR THE FUNCTIONING OF LUCIFERASE IN THE CELL
Колич.характеристики :5 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1994. - Vol. 59, Is. 7. - P761-765. - ISSN 0006-2979
Примечания : Cited References: 11
Предметные рубрики: BIOLUMINESCENT
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferase--nadh, fmn-oxidoreductase--immobilization
Аннотация: A gel model for the functioning of luciferase in cells has been constructed using bacterial NADH:FMN-oxidoreductase and luciferase immobilized in starch gel disks. The characteristics of the immobilized luciferase depend on the duration of drying, the amount and concentration of the gel, the nature of the support used for drying, and the properties of the initial enzyme preparation. Functionally important enzyme groups remain intact in the immobilized preparation, and luciferase retains its high specificity with respect to aldehydes. The gel microenvironment appears to be optimal for luciferase, judging from its high activity and increased stability. Conditions allowing repeated use of the preparation have been found. The approach permits co-immobilization of luciferase with other enzymes and their substrates. The error in bioluminescence measurements using the disks is 5-10%. A procedure for stabilization of the immobilized luciferase during repeated use has been devised.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., L'vova L.S., Egorova O.I., Kudryasheva N.S., Orlova N.Y., Bytev V.O.
Заглавие : Effect of Fusarium mycotoxins an a bacterial bioluminescence system in vitro
Колич.характеристики :3 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 1998. - Vol. 34, Is. 2. - P190-192. - ISSN 0003-6838
Примечания : Cited References: 7
Аннотация: The effects of the mycotoxins produced by fungi of the genus Fusarium on the NADH : Ravine mononucleotide oxidoreductase-luciferase bacterial bioluminescence system was studied. The sensitivity of the bioluminescence system to mycotoxins decreases in the following order: zearalenone, deoxynivalenol, toxin T-2, and diacetoxiscripenol. These results allow the development of a luciferase bioluminescence test system for rapid control of grain infection with Fusarium.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Egorova O.I., Esimbekova E.N., Kudryashova N.S., Orlova N.Y., L'vova L.S.
Заглавие : A biological luciferase test for the bioluminescent assay of wheat grain infection with Fusarium
Колич.характеристики :3 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 1998. - Vol. 34, Is. 6. - P622-624. - ISSN 0003-6838
Примечания : Cited References: 7
Аннотация: The extent of inhibition of the bioluminescence reaction by wheat grain extracts was studied as a function of the scabby kernel content in wheat. The NADH : flavine mononucleotide oxidoreductase-luciferase bienzyme bioluminescence system was found to be the most sensitive to mycotoxins produced by fungi of the genus Fusarium. A biological luciferase test was developed for monitoring wheat grain infection with Fusarium.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Kudinova I.Y., Esimbekova E.N., Kratasyuk V.A., Stom D.I.
Заглавие : The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems
Колич.характеристики :8 с
Место публикации : Chemosphere: PERGAMON-ELSEVIER SCIENCE LTD, 1999. - Vol. 38, Is. 4. - P751-758. - ISSN 0045-6535, DOI 10.1016/S0045-6535(98)00218-5
Примечания : Cited References: 7
Аннотация: Kinetics of the triple bioluminescent enzyme system: alcohol dehydrogenase - NADH:FMN-oxidoreductase - luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD(+)/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring. (C) 1998 Elsevier Science Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Vetrova E.V., Kudryasheva N.S.
Заглавие : Bioluminescent water quality monitoring of salt lake Shira
Колич.характеристики :3 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P193-195. - ISSN 1522-7235, DOI 10.1002/(SICI)1522-7243(199907/08)14:4193::AID-BIO5283.3.CO;2-J
Примечания : Cited References: 9
Ключевые слова (''Своб.индексиров.''): bioluminescence--biotest--ecological monitoring--salt lake
Аннотация: The coupled bioluminescent enzyme system luciferase-NADH:FMN-oxidoreductase was used as a biotest in ecological monitoring of the health resort salt lake Shira (South Siberia, Russia). The technique was adapted to saltwater conditions. Bioluminescence kinetic parameters sensitive to pollutants were determined. Conditions for the use of bacterial bioluminescence biotests in salty environmental media were established. Copyright (C) 1999 John Wiley & Sons, Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Esimbekova E.N., Kudinova I.Y., Kratasyuk V.A., Stom D.I.
Заглавие : Effects of quinones on NADH-dependent enzymatic bioluminescent systems
Колич.характеристики :5 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2000. - Vol. 36, Is. 4. - P409-413. - ISSN 0003-6838, DOI 10.1007/BF02738052
Примечания : Cited References: 13
Аннотация: The effects of a number of quinones on the bioluminescence characteristics of a three-component enzymatic system containing alcohol dehydrogenase, bacterial luciferase, and NADH-FMN oxidoreductase were studied to find the most sensitive kinetic parameters of the system intended to be used in biological testing. Both direct and back reactions catalyzed by alcohol dehydrogenase were studied in the presence and in the absence of quinones. The kinetic parameters of the bioluminescent system were found to depend on the redox potentials and concentrations of quinones. The quinone-induced effects were shown to be associated with changes in the NAD(+)/NADH ratio in the chain of NADH-dependent enzymes, The three-enzyme system based on alcohol dehydrogenase is suggested as a bioluminescence test for ecological monitoring of waste water.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Esimbekova E.N., Gladyshev M.I., Khromichek E.B., Kuznetsov A.M., Ivanova E.A.
Заглавие : The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems
Место публикации : Chemosphere. - 2001. - Vol. 42, Is. 8. - С. 909-915. - ISSN 00456535 (ISSN) , DOI 10.1016/S0045-6535(00)00177-6
Ключевые слова (''Своб.индексиров.''): alcohol dehydrogenase--bacterial luciferase--bioluminescence--blooming--pollution--trypsin--water toxicity--alcohol dehydrogenase--benzoquinone--luciferase--trypsin--aquatic ecosystem--bioluminescence--water quality--article--bacterium culture--bioluminescence--blue green alga--ecosystem--pond--seasonal variation--water pollution--water quality--benzoquinones--biological assay--cyanobacteria--ecosystem--environmental monitoring--eutrophication--fmn reductase--indicators and reagents--luminescent measurements--nadh, nadph oxidoreductases--water pollutants--russian federation--algae--bacteria (microorganisms)--chlorophyta--cyanobacteria--uncultured cyanobacterium
Аннотация: A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. В© 2001 Elsevier Science Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A., Pechurkin N.S.
Заглавие : On monitoring the bacterial component as an indicator of the state of small man-made ecosystems
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1605-1609. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00256-3
Ключевые слова (''Своб.индексиров.''): bacteria--ecosystems--substrates--intracellular substrate concentration--space research--catalase--oxidoreductase--artificial ecosystem--article--bacterial phenomena and functions--biomass--culture medium--ecosystem--enzymology--growth, development and aging--metabolism--microbiology--oxygen consumption--pseudomonas--bacterial physiology--biomass--catalase--culture media--ecosystem--oxidoreductases--oxygen consumption--pseudomonas--water microbiology
Аннотация: High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity - intracellular substrate concentration - estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gerasimova M.A., Kudryasheva N.S.
Заглавие : Effects of potassium halides on bacterial bioluminescence
Колич.характеристики :5 с
Место публикации : J. Photochem. Photobiol. B-Biol.: ELSEVIER SCIENCE SA, 2002. - Vol. 66, Is. 3. - P218-222. - ISSN 1011-1344, DOI 10.1016/S1011-1344(02)00240-3
Примечания : Cited References: 20
Предметные рубрики: COMPONENTS
SEWAGE
Ключевые слова (''Своб.индексиров.''): bioluminescence--halides--heavy atom--inhibition--activation
Аннотация: The effects of potassium halides KCl, KBr and KI on NADH:FMN-oxidoreductase-luciferase bioluminescent coupled enzyme system were studied. The influence of salt additions on bioluminescence intensity and bioluminescence light yield was investigated. The inhibition and activation parameters of the salts were calculated using their dependencies on concentration of the salts. The correlation between the inhibition of bioluminescence intensity and the halide mass was demonstrated: the inhibiting ability of the salts increases with the increase of atomic weight of the anions. The inhibition parameters increase and the activation parameters decrease, accordingly. (C) 2002 Elsevier Science B.V. All rights reserved.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Nemtseva E.V., Sizykh A.G., Kratasyuk V.A., Visser AJWG
Заглавие : Estimation of energy of the upper electron-excited states of the bacterial bioluminescent emitter
Колич.характеристики :5 с
Место публикации : J. Photochem. Photobiol. B-Biol.: ELSEVIER SCIENCE SA, 2002. - Vol. 68, Is. 02.03.2013. - P88-92. - ISSN 1011-1344, DOI 10.1016/S1011-1344(02)00360-3
Примечания : Cited References: 25
Предметные рубрики: MECHANISM
Ключевые слова (''Своб.индексиров.''): bioluminescence--electron-excited states--energy transfer
Аннотация: The hypothesis of activity of the upper electron-excited states of the bacterial bioluminescent emitter was verified using dye molecules as foreign energy acceptors. Six compounds were selected having fluorescent state energies ranging from 25 700 to 32 000 cm(-1) (anthracene, pyrene, 1.4-bis(5-phenyloxasol-2-yl)benzene (POPOP), p-bis(o-methylstyryl)benzene (MSB), 2-methoxy-naphtalene, p-terphenyl), exceeding that of the bioluminescent emitter (22 000 cm(-1)). Their absorption spectra do not overlap with the bioluminescence spectrum; the trivial light absorption and the intermolecular resonance S-S energy transfer were excluded. Bacterial bioluminescent spectra of the coupled enzyme system NADH:FMN-oxidoreductase-luciferase in the presence of MSB were presented as an example. The weak sensitized fluorescence of MSB was registered. The results obtained have confirmed the activity of the energetic precursor in the bacterial bioluminescence. Its energy can be located in the interval of 26 000-27 000 cm(-1). (C) 2002 Published by Elsevier Science B.V.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N..., Vetrova E..., Kuznetsov A..., Kratasyuk V..., Stom D...
Заглавие : Bioluminescence assays: Effects of quinones and phenols
Колич.характеристики :5 с
Место публикации : Ecotox. Environ. Safe.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2002. - Vol. 53, Is. 2. - P221-225. - ISSN 0147-6513, DOI 10.1006/eesa.2002.2214
Примечания : Cited References: 18
Ключевые слова (''Своб.индексиров.''): bioluminescence assays--quinones--phenols
Аннотация: The influence of a series of quinones and phenols on bacterial bioluminescence systems was investigated. Three bioluminescence systems used in ecological monitoring were compared: (1) water-soluble; (2) immobilized in starch gel coupled enzyme systems: NADH:FMN-oxidoreductase-luciferase; (3) luminescent bacteria. Bioluminescence inhibition constants of quinones and phenols and bioluminescence induction periods were compared. These kinetic parameters are proportional to quinone concentrations and depend on the quinone redox potential. Different effects of the substances are related to structure and properties of the bioluminescence systems. The set of bioluminescence assays for quinones and phenols monitoring should include two bioluminescence systems: 1 (or 2) and 3. (C) 2002 Elsevier Science (USA).
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Место публикации : Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - С. 675-678. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-002-0941-8
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyric acid--acetoacetyl coenzyme a reductase--acetyl coenzyme a acyltransferase--beta hydroxyvalerate--butyrate dehydrogenase--carbon monoxide--electrolyte--hydrogen--oxidoreductase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid)synthase--polyhydroxyalkanoic acid--polymer--unclassified drug--valeric acid--bacterium--article--autotrophy--bacterial growth--bacterial strain--biomass production--controlled study--crystallization--enzyme activity--molecular weight--nonhuman--synthesis--temperature--wautersia eutropha--carbon monoxide--culture media--cupriavidus necator--fatty acids--lipids--polyesters--bacteria (microorganisms)--negibacteria--ralstonia--wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Gorbunova O.V., Zhila N.O.
Заглавие : Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786
Место публикации : Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - С. 170-177. - ISSN 00036838 (ISSN) , DOI 10.1023/B:ABIM.0000018921.04863.d5
Ключевые слова (''Своб.индексиров.''): acetyl coenzyme a acyltransferase--bacterial enzyme--carbon--carbon dioxide--fructose--hydrogen--hydroxybutyrate dehydrogenase--oxidoreductase--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--synthetase--article--bacterial metabolism--carbon source--catalysis--controlled study--degradation--depolymerization--enzyme activity--enzyme analysis--molecular dynamics--nonhuman--protein function--ralstonia eutropha--recording--statistical significance--synthesis--bacteria (microorganisms)--ralstonia--wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vetrova E.V., Kudryasheva N.S., Visser AJWG, van Hoek A...
Заглавие : Characteristics of enclogenous flavin fluorescence of Photobacterium leiognathi luciferase and Vibrio fischeri NAD(P)H : FMN-oxidoreductase
Колич.характеристики :5 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2005. - Vol. 20: 11th International Symposium on Luminescence Spectrometry - Detection Techniques in Biomedical and Environmental Analysis (MAY 05-08, 2004, Beijing, JAPAN), Is. 3. - P205-209. - ISSN 1522-7235, DOI 10.1002/bio.815
Примечания : Cited References: 22
Предметные рубрики: FLAVODOXIN
ANISOTROPY
REDUCTASE
DYNAMICS
SYSTEM
Ключевые слова (''Своб.индексиров.''): bacterial bioluminescence--flavin fluorescence
Аннотация: The bioluminescent bacterial enzyme system NAD(P)H:FMN-oxidoreductase-luciferase has been used as a test system for ecological monitoring. One of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. The use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from Photobacterium leiognathi and NAD(P)H:FMN-oxidoreductase from Vibrio fischeri has been proposed. Fluorescence spectroscopy methods have been used to study characteristics of endogenous flavin fluorescence (fluorophore lifetime, the rotational correlation time). The fluorescence anisotropy behaviour of FMN has been analysed and compared to that of the enzyme-bound flavin. The fluorescence characteristics of endogenous flavin of luciferase and NAD(P)H:FMN-oxidoreductase have been shown to be applicable in studying enzymes' interactions with non-fluorescent compounds. Copyright (c) 2005 John Wiley & Sons, Ltd.
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20.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Esimbekova, Elena, Kratasyuk, Valentina, Shimomura, Osamu
Заглавие : Application of Enzyme Bioluminescence in Ecology
Колич.характеристики :43 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 67-109. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_3
Примечания : Cited References:85
Предметные рубрики: BACTERIAL LUCIFERASE
IN-VITRO
PYRETHROID INSECTICIDES
FRESH-WATER
Ключевые слова (''Своб.индексиров.''): bioluminescence--ecological monitoring--enzymatic assay--immobilization--integral water toxicity--luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.
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