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All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2 +-loaded apo-berovin / L. P. Burakova [et al.] // J. Photochem. Photobiol. B Biol. - 2016. - Vol. 154. - P57-66, DOI 10.1016/j.jphotobiol.2015.11.012 . - ISSN 1011-1344
Кл.слова (ненормированные):
Aequorin -- Bioluminescence -- Calcium -- Coelenterazine -- Obelin
Аннотация: Light-sensitive photoprotein berovin accounts for a bright bioluminescence of ctenophore Beroe abyssicola. Berovin is functionally identical to the well-studied Ca2+-regulated photoproteins of jellyfish, however in contrast to those it is extremely sensitive to the visible light. Berovin contains three EF-hand Ca2+-binding sites and consequently belongs to a large family of the EF-hand Ca2+-binding proteins. Here we report the spatial structure of apo-berovin with bound Mg2+ determined at 1.75 A. The magnesium ion is found in each functional EF-hand loop of a photoprotein and coordinated by oxygen atoms donated by the side-chain groups of aspartate, carbonyl groups of the peptide backbone, or hydroxyl group of serine with characteristic oxygen-Mg2+ distances. As oxygen supplied by the side-chain of the twelfth residue of all Ca2+-binding loops participates in the magnesium ion coordination, it was suggested that Ca2+-binding loops of berovin belong to the mixed Ca2+/Mg2+ rather than Ca2+-specific type. In addition, we report an effect of physiological concentration of Mg2+ on bioluminescence of berovin (sensitivity to Ca2+, rapid-mixed kinetics, light-sensitivity, thermostability, and apo-berovin conversion into active protein). The different impact of physiological concentration of Mg2+ on berovin bioluminescence as compared to hydromedusan photoproteins was attributed to different affinities of the Ca2 +-binding sites of these photoproteins to Mg2+. © 2015 Elsevier B.V. All rights reserved.

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Держатели документа:
Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok 50, Krasnoyarsk, Russian Federation
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing, China
IHuman Institute, ShanghaiTech University, 99 Haike Road, Shanghai, China

Доп.точки доступа:
Burakova, L. P.; Natashin, P. V.; Malikova, N. P.; Niu, F.; Pu, M.; Vysotski, E. S.; Liu, Z.-J.
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Oxygen and ammonia plasma treatment of poly(3-hydroxybutyrate) films for controlled surface zeta potential and improved cell compatibility [Text] / D. S. Syromotina [et al.] // Mater. Lett. - 2016. - Vol. 163. - P277-280, DOI 10.1016/j.matlet.2015.10.080. - Cited References:10. - The authors thank Mr. T.M Mukhametkaliyev, Mrs. A.A. Sharonova and Dr. A. Wittmar for their assistance with the experiments. This research was supported by the Russian President's Stipend SP-6664.2013.4, Ministry for Education and Science Grant MK-485.2014.8, the State order NAUKA #11.1359.2014/K, and the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 0167-577X. - ISSN 1873-4979

РУБ Materials Science, Multidisciplinary + Physics, Applied

Кл.слова (ненормированные):
Plasma treatment -- Polymer -- Cell adhesion -- Surface modification
Аннотация: The oxygen and ammonia radio-frequency (RF) plasma treatment of poly(3-hydroxybutyrate) P3HB films was performed. We revealed significant changes in the topography, a decrease in the surface zeta potential from -63 to -75 mV after the oxygen-plasma treatment and an increase after ammonia plasma treatment from -63 to -45 mV at a pH of 7.4. Investigations into the NIH 3T3 fibroblast adhesion and growth demonstrated the best cell vitality and a higher cell number for the ammonia plasma treatment at 150W. (C) 2015 Elsevier B.V. All rights reserved.

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Держатели документа:
Natl Res Tomsk Polytech Univ, Tomsk 634050, Russia.
Fraunhofer Inst Interfacial Engn & Biotechnol IGB, D-70569 Stuttgart, Germany.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Univ Duisburg Essen, Inorgan Chem, D-45117 Essen, Germany.
Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45117 Essen, Germany.
Univ Duisburg Essen, Tech Chem 2, D-45141 Essen, Germany.
Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45141 Essen, Germany.

Доп.точки доступа:
Syromotina, D. S.; Surmenev, R. A.; Surmeneva, M. A.; Boyandin, A. N.; Epple, M.; Ulbricht, M.; Oehr, C.; Volova, T. G.; Russian President's Stipend [SP-6664.2013.4]; Ministry for Education and Science Grant [MK-485.2014.8]; State order NAUKA [11.1359.2014/K]; State budget [01201351505]

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Characteristics of oxygen transport through the surface of the isolated perfused rat liver / K. V. Shadrin [et al.] // Doklad. Biochem. Biophys. - 2015. - Vol. 464, Is. 1. - P298-300, DOI 10.1134/S1607672915050075 . - ISSN 1607-6729
Аннотация: It is shown that the transport of oxygen through the surface of the isolated perfused rat liver is an energy-dependent process that requires the energy of ATP hydrolysis. © 2015, Pleiades Publishing, Ltd.

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Держатели документа:
Krasnoyarsk Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, Russian Federation
Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Krasnoyarsk Krai, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Shadrin, K. V.; Morgulis, I. I.; Pahomova, V. G.; Rupenko, A. P.; Khlebopros, R. G.

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Field emission luminescence of nanodiamonds deposited on the aligned carbon nanotube array [Text] / Y. V. Fedoseeva [et al.] // Sci Rep. - 2015. - Vol. 5. - Ст. 9379, DOI 10.1038/srep09379. - Cited References:49. - The work was supported by RFBR grant 13-03-12118 in the part of electroluminescence measurements and the bilateral Program "Russian-German Laboratory at BESSY''. We are grateful to Mr. A.V. Ischenko for the TEM measurements, Mr. S.I. Kozhemyachenko for the Raman spectra, Mrs. N.I. Alferova for the IR spectra, and Mr. D.V. Gulyaev for the photoluminescence spectra. . - ISSN 2045-2322

РУБ Multidisciplinary Sciences
Рубрики:
DETONATION NANODIAMOND
ULTRANANOCRYSTALLINE DIAMOND
Аннотация: Detonation nanodiamonds (NDs) were deposited on the surface of aligned carbon nanotubes (CNTs) by immersing a CNT array in an aqueous suspension of NDs in dimethylsulfoxide (DMSO). The structure and electronic state of the obtained CNT-ND hybrid material were studied using optical and electron microscopy and Infrared, Raman, X-ray photoelectron and near-edge X-ray absorption fine structure spectroscopy. A non-covalent interaction between NDs and CNT and preservation of vertical orientation of CNTs in the hybrid were revealed. We showed that current-voltage characteristics of the CNT-ND cathode are changed depending on the applied field; below similar to 3 V/mu m they are similar to those of the initial CNT array and at the higher field they are close to the ND behavior. Involvement of the NDs in field emission process resulted in blue luminescence of the hybrid surface at an electric field higher than 3.5 V/mu m. Photoluminescence measurements showed that the NDs emit blue-green light, while blue luminescence prevails in the CNT-ND hybrid. The quenching of green luminescence was attributed to a partial removal of oxygen-containing groups from the ND surface as the result of the hybrid synthesis.

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Держатели документа:
Nikolaev Inst Inorgan Chem SB RAS, Novosibirsk 630090, Russia.
Novosibirsk State Univ, Novosibirsk 630090, Russia.
Tech Univ Dresden, Inst Solid State Phys, D-01062 Dresden, Germany.
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
ИБФ СО РАН

Доп.точки доступа:
Fedoseeva, Yu. V.; Bulusheva, L.G.; Okotrub, A.V.; Kanygin, M.A.; Gorodetskiy, D.V.; Asanov, I.P.; Vyalikh, D.V.; Puzyr, A.P.; Bondar, V.S.; RFBR grant [13-03-12118]; bilateral Program "Russian-German Laboratory at BESSY''

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Effect of low-dose ionizing radiation on luminous marine bacteria: radiation hormesis and toxicity [Text] / N. S. Kudryasheva, T. V. Rozhko // J. Environ. Radioact. - 2015. - Vol. 142. - P68-77, DOI 10.1016/j.jenvrad.2015.01.012. - Cited References:131. - This work was supported by the Russian Foundation for Basic Research, Grant No.13-04-01305a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1. The part of the work (review of effects of americium-241) was supported by the Russian Science Foundation, Grant No. 14-14-00076. . - ISSN 0265-931X. - ISSN 1879-1700

РУБ Environmental Sciences
Рубрики:
RECOMBINANT LUMINESCENT MICROORGANISMS
PHOTOBACTERIUM-LEIOGNATHI
Кл.слова (ненормированные):
Marine bacteria -- Low-dose effects -- Radiation hormesis -- Radiotoxicity -- Reactive oxygen species
Аннотация: The paper summarizes studies of effects of alpha- and beta-emitting radionuclides (americium-241, uranium-235+238, and tritium) on marine microorganisms under conditions of chronic low-dose irradiation in aqueous media. Luminous marine bacteria were chosen as an example of these microorganisms; bioluminescent intensity was used as a tested physiological parameter. Non-linear dose-effect dependence was demonstrated. Three successive stages in the bioluminescent response to americium-241 and tritium were found: 1 - absence of effects (stress recognition), 2 - activation (adaptive response), and 3 - inhibition (suppression of physiological function, i.e. radiation toxicity). The effects were attributed to radiation hormesis phenomenon. Biological role of reactive oxygen species, secondary products of the radioactive decay, is discussed. The study suggests an approach to evaluation of non-toxic and toxic stages under conditions of chronic radioactive exposure. (C) 2015 Elsevier Ltd. All rights reserved.

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Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Krasnoyarsk 660041, Russia
Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia

Доп.точки доступа:
Kudryasheva, N. S.; Rozhko, T. V.; Russian Foundation for Basic Research [13-04-01305a]; Program "Molecular and Cellular Biology" of the Russian Academy of Sciences [VI 57.1.1]; Russian Science Foundation [14-14-00076]

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Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma / D. S. Syromotina [et al.] // Mater. Sci. Eng. C. - 2016. - Vol. 62. - P450-457, DOI 10.1016/j.msec.2016.01.075 . - ISSN 0928-4931
Кл.слова (ненормированные):
Plasma -- Polar component -- poly-3-hydroxybutyrate membranes -- Surface energy -- Surface treatment -- Ammonia -- Biodegradable polymers -- Cell adhesion -- Contact angle -- Free energy -- Interfacial energy -- Plasma applications -- Plasmas -- Surface chemistry -- Surface properties -- Surface roughness -- Surface topography -- Wetting -- Biological performance -- Crystalline structure -- Polar components -- Poly-3-hydroxybutyrate -- Surface free energy -- Surface roughness changes -- Surface wettability -- Water contact angle -- Surface treatment
Аннотация: The surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer, with the exception being a power level of 250 W. The wettability of the modified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. It was revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma. © 2016 Elsevier B.V.

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Держатели документа:
Department of Experimental Physics, National Research Tomsk Polytechnic University, Tomsk, Russian Federation
Fraunhofer Institute for Interfacial Engineering and Biotechnology, IGB, Stuttgart, Germany
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, Russian Federation
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany
Technical Chemistry II and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany

Доп.точки доступа:
Syromotina, D. S.; Surmenev, R. A.; Surmeneva, M. A.; Boyandin, A. N.; Nikolaeva, E. D.; Prymak, O.; Epple, M.; Ulbricht, M.; Oehr, C.; Volova, T. G.

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Why does the bioluminescent fungus Armillaria mellea have luminous mycelium but nonluminous fruiting body? / K. V. Purtov [et al.] // Doklad. Biochem. Biophys. - 2017. - Vol. 474, Is. 1. - P217-219, DOI 10.1134/S1607672917030176 . - ISSN 1607-6729
Аннотация: By determining the components involved in the bioluminescence process in luminous and nonluminous organs of the honey fungus Armillaria mellea, we have established causes of partial luminescence of this fungus. The complete set of enzymes and substrates required for bioluminescence is formed only in the mycelium and only under the conditions of free oxygen access. Since the synthesis of luciferin precursor (hispidin) and 3-hydroxyhispidin hydroxylase in the fruiting bodies is blocked, the formation of luciferin—the key component of fungal bioluminescent system—was not observed. That is why the fruiting body of Armillaria mellea is nonluminous despite the presence of luciferase, the enzyme that catalyzes the oxidation of luciferin with a photon emission. © 2017, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Krasnoyarsk Research Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Gitelson, J. I.

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Morphological properties and levels of extracellular peroxidase activity and light emission of the basidiomycete Armillaria borealis treated with beta-glucosidase and chitinase / O. A. Mogilnaya [et al.] // Mycosphere. - 2017. - Vol. 8, Is. 4. - P649-+, DOI 10.5943/mycosphere/8/4/11. - Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project no. 0356-2016-0709) and Program No. II. 2 "Integration and Development" of the Siberian Branch of the Russian Academy of Sciences (project no. 0356-2015-0103). . - ISSN 2077-7000

РУБ Mycology
Рубрики:
FUNGAL CELL-WALL
OXIDATIVE STRESS
PHANEROCHAETE-CHRYSOSPORIUM
Кл.слова (ненормированные):
basidiomycetes -- bioluminescence -- cell wall -- beta-glucosidase -- chitinase -- peroxidase
Аннотация: The study estimates morphological properties and levels of extracellular peroxidase activity and light emission of mycelium of the basidiomycete Armillaria borealis IBSO 2328 treated with beta-glucosidase and chitinase. Mycelium incubated with the enzymes shows considerable morphological changes and indications of osmotic shock. Injuries observed in the cell envelope of the fungal hyphae are primarily attributed to the partial (in the beta-glucosidase treatment) or complete (in the chitinase treatment) disintegration of the melanin layer on the surface of the cell wall. Changes in the cell wall of hyphae are accompanied by release of extracellular peroxidases of the fungus into the incubation medium and an increase in light emission relative to the luminescence of the control pellets. We assume that higher level of luminescence of the enzyme-treated mycelium samples could be related to the disintegration of the surface pigment layer of the hyphae and the partial loss of extracellular peroxidases. The data obtained confirm the previously proposed hypothesis in which light producing reaction of the fungus may be an additional way to neutralize active oxygen radicals under stress.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Mogilnaya, O. A.; Ronzhin, N. O.; Artemenko, K. S.; Bondar, V. S.; Russian Academy of Sciences [0356-2016-0709, 0356-2015-0103]

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Mechanism and color modulation of fungal bioluminescence / Z. M. Kaskova [et al.] // Sci. Adv. - 2017. - Vol. 3, Is. 4. - Ст. e1602847, DOI 10.1126/sciadv.1602847. - Cited References:40. - This work was supported by the Sao Paulo Research Foundation [FAPESP grants 10/11578-5 (to A.G.O.), 13/16885-1 (to C.V.S.), 14/14866-2 (to E.L.B.), 13/07914-8 (to E.P. and F.A.D.), and 2012/12663-1 (to P.D.M.) and CEPID Redoxoma 2013/07937-8 (to P.D.M.)], the National Council for Scientific and Technological Development (CNPq) [301307/2013-0 (to P.D.M.)], NAP Redoxoma (PRPUSP) [2011.1.9352.1.8. (to P.D.M.)], the Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) [grant no. 16K07715 (to Y.O.)], Chubu University [grant AII28II M01 (to Y.O.)], and the Russian Science Foundation (grant 16-14-00052 to all Russian authors). . - ISSN 2375-2548

РУБ Multidisciplinary Sciences
Рубрики:
SINGLET MOLECULAR-OXYGEN
QUANTUM YIELDS
CHEMILUMINESCENCE
Аннотация: Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates.

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Держатели документа:
Russian Acad Sci, Inst Bioorgan Chem, Miklukho Maklaya 16-10, Moscow 117997, Russia.
Pirogov Russian Natl Res Med Univ, OStrovitianov 1, Moscow 117997, Russia.
SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicolgicas, BR-05508900 Sao Paulo, Brazil.
Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan.
Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo, Brazil.
Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508900 Sao Paulo, Brazil.
Univ Sao Paulo, Inst Oceanografico, Dept Oceanografia Fis Quim & Geol, BR-05508120 Sao Paulo, Brazil.
Chubu Univ, Dept Environm Biol, Kasugai, Aichi 4878501, Japan.

Доп.точки доступа:
Kaskova, Zinaida M.; Dorr, Felipe A.; Petushkov, Valentin N.; Purtov, Konstantin V.; Tsarkova, Aleksandra S.; Rodionova, Natalja S.; Mineev, Konstantin S.; Guglya, Elena B.; Kotlobay, Alexey; Baleeva, Nadezhda S.; Baranov, Mikhail S.; Arseniev, Alexander S.; Gitelson, Josef I.; Lukyanov, Sergey; Suzuki, Yoshiki; Kanie, Shusei; Pinto, Ernani; Di Mascio, Paolo; Waldenmaier, Hans E.; Pereira, Tatiana A.; Carvalho, Rodrigo P.; Oliveira, Anderson G.; Oba, Yuichi; Bastos, Erick L.; Stevani, Cassius V.; Yampolsky, Ilia V.; Sao Paulo Research Foundation [FAPESP] [10/11578-5, 13/16885-1, 14/14866-2, 13/07914-8, 2012/12663-1]; CEPID Redoxoma [2013/07937-8]; National Council for Scientific and Technological Development (CNPq) [301307/2013-0]; NAP Redoxoma (PRPUSP) [2011.1.9352.1.8]; Japan Society for the Promotion of Science [16K07715]; Chubu University [AII28II M01]; Russian Science Foundation [16-14-00052]

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10.

Bacterial Communities of Three Saline Meromictic Lakes in Central Asia [Text] / B. Baatar [et al.] // PLoS One. - 2016. - Vol. 11, Is. 3. - Ст. e0150847, DOI 10.1371/journal.pone.0150847. - Cited References:65. - This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99- 2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a.; We thank our Russian and Mongolian colleagues in the Institute of Biophysics SB RAS and National University of Mongolia for assistance with providing samples and hydro-parameter data. This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99-2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
PURPLE SULFUR BACTERIA
REAL-TIME PCR
MICROBIAL COMMUNITIES
Аннотация: Meromictic lakes located in landlocked steppes of central Asia (similar to 2500 km inland) have unique geophysiochemical characteristics compared to other meromictic lakes. To characterize their bacteria and elucidate relationships between those bacteria and surrounding environments, water samples were collected from three saline meromictic lakes (Lakes Shira, Shunet and Oigon) in the border between Siberia and the West Mongolia, near the center of Asia. Based on in-depth tag pyrosequencing, bacterial communities were highly variable and dissimilar among lakes and between oxic and anoxic layers within individual lakes. Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria and Firmicutes were the most abundant phyla, whereas three genera of purple sulfur bacteria (a novel genus, Thiocapsa and Halochromatium) were predominant bacterial components in the anoxic layer of Lake Shira (similar to 20.6% of relative abundance), Lake Shunet (similar to 27.1%) and Lake Oigon (similar to 9.25%), respectively. However, few known green sulfur bacteria were detected. Notably, 3.94% of all sequencing reads were classified into 19 candidate divisions, which was especially high (23.12%) in the anoxic layer of Lake Shunet. Furthermore, several hydro-parameters (temperature, pH, dissolved oxygen, H2S and salinity) were associated (P0.05) with variations in dominant bacterial groups. In conclusion, based on highly variable bacterial composition in water layers or lakes, we inferred that the meromictic ecosystem was characterized by high diversity and heterogenous niches.

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Держатели документа:
Acad Sinica, Taiwan Int Grad Program, Mol & Biol Agr Sci Program, Taipei 115, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan.
Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan.
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia.
Natl Pingtung Univ Sci & Technol, Pingtung 91201, Taiwan.
Natl Univ Mongolia, Sch Art & Sci, Ulaanbaatar 14201, Mongol Peo Rep.
Natl Chung Hsing Univ, Ctr Biotechnol, Taichung 40227, Taiwan.

Доп.точки доступа:
Baatar, Bayanmunkh; Chiang, Pei-Wen; Rogozin, Denis Yu; Wu, Yu-Ting; Tseng, Ching-Hung; Yang, Cheng-Yu; Chiu, Hsiu-Hui; Oyuntsetseg, Bolormaa; Degermendzhy, Andrey G.; Tang, Sen-Lin; National Sciences Council of Taiwan [NSC99- 2923-B-001-001-MY3, NSC 102-2923-B-001-004, NSC101-2923-B-001-003-MY3, NSC99-2923-B-001-001-MY3]; Biodiversity Research Center, Academia Sinica, Taiwan; Russian Foundation for Basic Research [14-04-01060-a]

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11.

Progress in the Study of Bioluminescent Earthworms / N. S. Rodionova [et al.] // Photochem. Photobiol. - 2017. - Vol. 93, Is. 2. - P416-428, DOI 10.1111/php.12709 . - ISSN 0031-8655
Аннотация: Even though bioluminescent oligochaetes rarely catch people's eyes due to their secretive lifestyle, glowing earthworms sighting reports have come from different areas on all continents except Antarctica. A major breakthrough in the research of earthworm bioluminescence occurred in the 1960s with the studies of the North American Diplocardia longa. Comparative studies conducted on 13 earthworm species belonging to six genera showed that N-isovaleryl-3-aminopropanal (Diplocardia luciferin) is the common substrate for bioluminescence in all examined species, while luciferases appeared to be responsible for the color of bioluminescence. The second momentous change in the situation has occurred with the discovery in Siberia (Russia) of two unknown luminous enchytraeids. The two bioluminescent systems belong to different types, have different spectral characteristics and localization, and different temperature and pH optima. They are unique, and this fact is confirmed by the negative results of all possible cross-reactions. The bioluminescent system of Henlea sp. comprises four essential components: luciferase, luciferin, oxygen and calcium ion. For Friderica heliota, the luminescent reaction requires five components: luciferase, luciferin, ATP, magnesium ion and oxygen. Along with luciferin, more than a dozen analogues were isolated from worm biomass. These novel peptide-like natural compounds represent an unprecedented chemistry found in terrestrial organisms. © 2017 The American Society of Photobiology

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Держатели документа:
Laboratory of Photobiology, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Department of Physics, Earth and Environmental Sciences, University of Siena, Siena, Italy
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Pirogov Russian National Research Medical University, Moscow, Russian Federation

Доп.точки доступа:
Rodionova, N. S.; Rota, E.; Tsarkova, A. S.; Petushkov, V. N.

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12.

Interrelations of epibiontic microalgae and crustacean zooplankton under conditions of a blooming eutrophic water body [Text] / N. A. Gaevskii [et al.] // Russ. J. Ecol. - 2004. - Vol. 35, Is. 1. - P. 35-41, DOI 10.1023/B:RUSE.0000011107.72097.1c. - Cited References: 22 . - ISSN 1067-4136

РУБ Ecology
Рубрики:
ALGAL EPIBIONT
   DAPHNIA
   HOST
   COMMUNITIES
Кл.слова (ненормированные):
epibiontic microalgae -- Cyclops vicinus -- Daphnia longispina -- mutualism -- algal bloom
Аннотация: The possibility of symbiotic, mutualistic (++) interrelations between epibiontic microalgae (Characidiopsis ellipsoidea, Colacium vesiculosum) and cyclopoids (Cyclops vicinus, Mesocyclops leukarti) in the Bugach recreational reservoir with a bloom of cyanobacteria is demonstrated. Overgrown cyclopoids, compared to those free from microalgae, have advantages in oxygen supply, which may be insufficient in the period of intensive algal bloom. Calculations have shown that oxygen requirements of cyclopoids may be fully satisfied due to C. vesiculosum photosynthetic activity in the dysphotic zone if the density of overgrowth exceeds 1000 algal cells per individual. There is no significant correlation between the natural mortality of crustaceans and the indices of their overgrowth by microalgae.

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Держатели документа:
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevskii, N.A.; Kolmakov, V.I.; Dubovskaya, O.P.; Klimova, E.P.

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13.

Mineralization of wastes of human vital activity and plants in LSS [Text] / Y. A. Kudenko, I. V. Gribovskaya, R. A. Pavlenko ; ed. ya, IV Gribovs // SIXTH EUROPEAN SYMPOSIUM ON SPACE ENVIRONMENTAL CONTROL SYSTEMS, VOLS 1 AND 2. Ser. ESA SPECIAL PUBLICATIONS : EUROPEAN SPACE AGENCY, 1997. - Vol. 400: 6th European Symposium on Space Environmental Control Systems (MAY 20-22, 1997, NOORDWIJK, NETHERLANDS). - P. 803-806. - Cited References: 0 . - ISBN 0379-6566. - ISBN 92-9092-283-4

РУБ Engineering, Aerospace

Аннотация: Available methods for mineralizing wastes of human activity and inedible biomass of plants used in this country and abroad are divided into two types: dry mineralization at high temperatures up to 1270 degrees K with subsequent partial dissolution of the ash and the other wet oxidation by acids. In this case mineralization is performed at the temperature of 470-460 degrees K and the pressure of 220-270 atmospheres in pure oxygen with the output of mineral solution and dissoluble sediments in the form of scale. The drawback of the former method is formation of dioxins, CO, SO2, NO2 and other toxic compounds. The latter method is too sophisticated and presently is confined to bench testing. The proposed method to mineralize the wastes is in mid-position between the thermal and physical chemical methods. At the temperature of 80-90 degrees C the mixture was exposed to controlled electromagnetic field at normal atmospheric pressure. The method merits simplicity, reliability, produces no dissoluble sediment, emissions noxious for humans and plants. The basic difference from the above said methods is to employ for oxidizer atomic oxygen, its active forms including OH-radicals with hydrogen peroxide as the source. Hydrogen peroxide can be produced with electric power from water inside LSS.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudenko, Y.A.; Gribovskaya, I.V.; Pavlenko, R.A.; Gribovs, ya, IV \ed.\

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14.

Cotranslational formation of active photoprotein obelin in a cell-free translation system: Direct ultrahigh sensitive measure of the translation course [Text] / N. G. Berestovskaya [et al.] // Anal. Biochem. - 1999. - Vol. 268, Is. 1. - P. 72-78, DOI 10.1006/abio.1998.3051. - Cited References: 22 . - ISSN 0003-2697

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Chemistry, Analytical
Рубрики:
SEQUENCE-ANALYSIS
   MESSENGER-RNA
   CA-2+-ACTIVATED PHOTOPROTEIN
   LIGHT-EMISSION
   AEQUORIN
   CDNA
   CLONING
   EXPRESSION
Аннотация: Translation of apoobelin mRNA in a cell-free wheat germ translation system in the presence of coelenterazine and molecular oxygen results in cotranslational formation of active photoprotein. Active obelin formation is recorded by its luminescence, either direct in the translation mixture in the presence of coelenterazine and calcium ions or in aliquots from the translation mixture. In the second case translation is carried out with coelenterazine and EGTA. Registration of the translation course by luminescence of the synthesized product in both cases allows use of apoobelin mRNA at very low concentrations as an internal marker for immediate measure of protein biosynthesis activity of in vitro translation systems. It is shown that the simultaneous translation of any other mRNA does not affect translation of photoprotein mRNAs under standard conditions. Continuous registration of luminescence in a cuvette of a liquid scintillation counter in photon-counting mode varies the time of signal accumulation in a wide temporal range, thus increasing the numerical values of the recorded signals. Registration of photoprotein luminescence during translation can be used to obtain additional information about the translation process, for example codon reading speed, about protein folding, and about the formation of active proteins on ribosomes. (C) 1999 Academic Press.

WOS
Держатели документа:
Russian Acad Sci, Branch Inst Bioorgan Chem, Pushchino 142292, Russia
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Tech Univ Berlin, Inst Biochem & Mol Biol, D-10587 Berlin, Germany
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Berestovskaya, N.G.; Shaloiko, L.A.; Gorokhovatsky, A.Y.; Bondar, V.S.; Vysotski, E.S.; Maximov, J.E.; von Doehren, H...; Alakhov, Y.B.

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15.

Mineralization of wastes of human vital activity and plants to be used in a life support system [Text] / Y. A. Kudenko, I. V. Gribovskaya, R. A. Pavlenko // Acta Astronaut. - 1997. - Vol. 41, Is. 3. - P. 193-196, DOI 10.1016/S0094-5765(97)00215-4. - Cited References: 8 . - ISSN 0094-5765

РУБ Engineering, Aerospace

Аннотация: Available methods for mineralizing wastes of human activity and inedible biomass of plants used in this country and abroad are divided into two types: dry mineralization at high temperatures up to 1270 K with subsequent partial dissolution of the ash and the other-wet oxidation by acids. In this case mineralization is performed at a temperature of 470-460 K and a pressure of 220-270 atmospheres in pure oxygen with the output of mineral solution and dissoluble sediments in the form of scale. The drawback of the first method is the formation of dioxins, CO, SO2, NO2 and other toxic compounds. The latter method is too sophisticated and is presently confined to bench testing. The here proposed method to mineralize the wastes is in mid-position between the thermal and physical chemical methods. At a temperature of 89-90 degrees C the mixture was exposed to a controlled electromagnetic field at normal atmospheric pressure. The method merits simplicity, reliability, produces no dissoluble sediment or emissions noxious for human and plants. The basic difference from the above said methods is to employ as an oxidizer atomic oxygen, its active forms including OH-radicals with hydrogen peroxide as the sourer. Hydrogen peroxide can be produced with electric power from water inside the Life Support System (LSS). (C) 1998 Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudenko, Y.A.; Gribovskaya, I.V.; Pavlenko, R.A.

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16.

EFFECT OF CULTIVATION PARAMETERS ON THE LEVEL OF HYDROGENASE ACTIVITY IN ALCALIGENES-EUTROPHUS Z-1 CELLS [Text] / O. A. GUSEINOV, V. F. PLOTNIKOV // Microbiology. - 1991. - Vol. 60, Is. 2. - P. 144-148. - Cited References: 13 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
SOLUBLE HYDROGENASE
BACTERIA
Аннотация: The effect of different cultivation parameters on the specific activities of soluble and membrane-bound hydrogenases was studied in Alcaligenes eutrophus Z-1 cells. These activities were shown to be dependent primarily on the availability of electrons from the substrate used. Changes in cultivation temperature and oxygen concentration versus the optimal values as well as addition of low amounts of carbon monoxide to the gas phase exerted a lesser effect on the specific hydrogenase activities. Autotrophic and heterotrophic methods were developed for growing A. eutrophus Z-1 cells with a high hydrogenase activity.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GUSEINOV, O.A.; PLOTNIKOV, V.F.

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17.

Oxidation of elemental sulfur by Thiobacillus ferrooxidans [Text] / A. V. Belyi [et al.] // Appl. Biochem. Microbiol. - 1997. - Vol. 33, Is. 5. - P. 503-506. - Cited References: 10 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: In batch cultures of Thiobacillus ferrooxidans, the initial linear phase of sulfur oxidation was followed by the exponential one. It was shown that both free cells and cells adsorbed to the substrate were involved in the oxidation. Initially, only cells adsorbed to the substrate consumed oxygen. During the exponential phase, they accounted for only half the oxygen consumed; the other half was consumed by free cells. After the cells adsorbed to the substrate were removed, the oxygen consumption by free cells decreased significantly.

WOS
Держатели документа:
RUSSIAN ACAD SCI,SIBERIAN DIV,INST BIOPHYS,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Belyi, A.V.; Gurevich, Y.L.; Pustoshilov, P.P.; Kadochnikova, G.G.

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18.

Evaluation of the effect of light intensity on the measurement of the photosynthetic rate in plankton microalgae by the chlorophyll fluorescence method [Text] / N. A. Gaevskii [et al.] // Russ. J. Plant Physiol. - 2000. - Vol. 47, Is. 6. - P. 820-825, DOI 10.1023/A:1026671531500. - Cited References: 21 . - ISSN 1021-4437

РУБ Plant Sciences
Рубрики:
QUANTUM YIELDS
   PHYTOPLANKTON
   CHLOROPLASTS
   ALGAE
Кл.слова (ненормированные):
fluorescence -- chlorophyll -- microalgae -- photosynthetic rate -- photosynthetic activity
Аннотация: The use of relative variable fluorescence (RVF) of chlorophyll, as measured in the presence of Diuron, an inhibitor of electron transfer, for the estimation of the photosynthetic activity of plankton microalgae was analyzed under a wide range of light intensities in the PAR region. Oxygen evolution rates (estimated by the method of light and dark bottles and the amperometric method), RVF, and chlorophyll a concentration were measured in parallel in natural algal cenoses and microecosystems. When the previously used regression equation, in the form A = b(DeltaF/F-d)CchlI, where A is O-2 evolution rate (g/(m(3) h), DeltaF/F-d is RVF (relative units), C-chl is chlorophyll a concentration (mg/m(3)), and I is light intensity (W/m(2)), was verified in the PAR region, we observed a nonlinear dependence of the correction coefficient b on I, which can be described by the formula b = 6.227 x 10(3)rootI. This result agrees with the hypothesis that chlorophyll a fluorescence quenching comprises photochemical (qQ) and energy (qE) components. On the basis of the energy model, we determined the upper limit b(max) = 0.003 for light intensity range I < 4.4 W/m(2) and the lower limit b(min) = 0.0003 for I = 400 W/m(2).

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Держатели документа:
Krasnoyarsk State Univ, Dept Biol, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevskii, N.A.; Kolmakov, V.I.; Popel'nitskii, V.A.; Gold, V.M.; Dubovskaya, O.P.

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19.

Competition of the natural and manmade biotic cycles in the closed aquatic system [Text] / T. I. Pisman, L. A. Somova ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1757-1761, DOI 10.1016/S0273-1177(03)00117-0. - Cited References: 10 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: This study addresses competition between the Paramecium bursaria and zoochlorella - endosymbiosis and the infusoria Paramecium caudatum in a closed aquatic system. The system is a natural model of a simple biotic cycle. P. bursaria consumes glucose and oxygen released by its zoochlorella and releases nitrogenous compounds and carbon dioxide necessary for algal photosynthesis. P. caudatum was fed on bacteria. It was shown that the infusoria P. bursaria united in one cycle with Chlorella had a higher competitive ability than P. caudatum. With any initial percentage of the infusoria in the mixed culture, the end portion of P. bursaria reached 90 - 99%, which was significantly higher than the end portion of the P. caudatum population. It is assumed that the sustenance expenditures of P. caudatum were greater than those of the endosymbiotic paramecium, i.e. the closing of the components into a biotic cycle leads to a decrease in sustenance expenditures. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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20.

Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1711-1720, DOI 10.1016/S0273-1177(03)00108-X. - Cited References: 13 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system, The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia
Univ Strasbourg 1, Clermont Ferrand, France
ESA, Estec, Environm Control & Life Support Sect, Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Y.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Y.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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