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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Высоцкий, Евгений Степанович, Бондарь, Владимир Станиславович, Трофимов К. П., Гительзон, Иосиф Исаевич
Заглавие : Люминесценция Ca{2}{+}-активируемого фотопротеина обелина под действием активных форм кислорода : научное издание
Место публикации : Докл. АН СССР. - 1991. - Т. 321, N 4. - С. 850-854. - ISSN 0002-3264
ГРНТИ : 34.17.09
Предметные рубрики: БЕЛОК
ОБЕЛИН
КАЛЬЦИЙ-АКТИВИРУЕМЫЙ
ЛЮМИНЕСЦЕНЦИЯ
КИСЛОРОД АКТИВНЫЙ
КИШЕЧНОПОЛОСТНЫЕ
PROTEIN
LUMINESCENCE
ACTION OXYGEN
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K. V., Petushkov V. N., Rodionova N. S., Gitelson J. I.
Заглавие : Why does the bioluminescent fungus Armillaria mellea have luminous mycelium but nonluminous fruiting body?
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 474, Is. 1. - С. 217-219. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672917030176
Аннотация: By determining the components involved in the bioluminescence process in luminous and nonluminous organs of the honey fungus Armillaria mellea, we have established causes of partial luminescence of this fungus. The complete set of enzymes and substrates required for bioluminescence is formed only in the mycelium and only under the conditions of free oxygen access. Since the synthesis of luciferin precursor (hispidin) and 3-hydroxyhispidin hydroxylase in the fruiting bodies is blocked, the formation of luciferin—the key component of fungal bioluminescent system—was not observed. That is why the fruiting body of Armillaria mellea is nonluminous despite the presence of luciferase, the enzyme that catalyzes the oxidation of luciferin with a photon emission. © 2017, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotski E.S., Liu Z.J., Markova S.V., Blinks J.R., Deng L..., Frank L.A., Herko M..., Malikova N.P., Rose J.P., Wang B.C., Lee J...
Заглавие : Violet bioluminescence and fast kinetics from W92F obelin: Structure-based proposals for the bioluminescence triggering and the identification of the emitting species
Колич.характеристики :12 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 2003. - Vol. 42, Is. 20. - С. 6013-6024. - ISSN 0006-2960, DOI 10.1021/bi027258h
Примечания : Cited References: 45
Предметные рубрики: RAY CRYSTALLOGRAPHIC ANALYSIS
PHOTOPROTEIN AEQUORIN
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
CALCIUM
LUMINESCENCE
LONGISSIMA
EVOLUTION
PROTEINS
COELENTERAZINE
Аннотация: Obelin from the hydroid Obelia longissima and aequorin are members of a subfamily of Ca2+-regulated photoproteins that is a part of the larger EF-hand calcium binding protein family. On the addition of Ca2+, obelin generates a blue bioluminescence emission (lambda(max) = 485 nm) as the result of the oxidative decarboxylation of the bound substrate, coelenterazine. The W92F obelin mutant is noteworthy because of the unusually high speed with which it responds to sudden changes of [Ca2+] and because it emits violet light rather than blue due to a prominent band with lambda(max) = 405 nm. Increase of pH in the range from 5.5 to 8.5 and using D2O both diminish the contribution of the 405 nm band, indicating that excited state proton transfer is involved. Fluorescence model studies have suggested the origin of the 485 nm emission as the excited state of an anion of coelenteramide, the bioluminescence reaction product, and 405 nm from the excited neutral state. Assuming that the dimensions of the substrate binding cavity do not change during the excited state formation, a His22 residue within hydrogen bonding distance to the 6-(p-hydroxy)-phenyl group of the excited coelenteramide is a likely candidate for accepting the phenol proton to produce an ion-pair excited state, in support of recent suggestions for the bioluminescence emitting state. The proton transfer could be impeded by removal of the Trp92 H-bond, resulting in strong enhancement of a 405 nm band giving the violet color of bioluminescence. Comparative analysis of 3D structures of the wild-type (WT) and W92F obelins reveals that there are structural displacements of certain key Ca2+-ligating residues in the loops of the two C-terminal EF hands as well as clear differences in hydrogen bond networks in W92F. For instance, the hydrogen bond between the side-chain oxygen atom of Asp 169 and the main-chain nitrogen of Arg112 binds together the incoming alpha-helix of loop III and the exiting cc-helix of loop IV in WT, providing probably concerted changes in these EF hands on calcium binding. But this linkage is not found in W92F obelin. These differences apparently do not change the overall affinity to calcium of W92F obelin but may account for the kinetic differences between the WT and mutant obelins. From analysis of the hydrogen bond network in the coelenterazine binding cavity, it is proposed that the trigger for bioluminescence reaction in these Ca2+-regulated photoproteins may be a shift of the hydrogen bond donor-acceptor separations around the coelenterazine-2-hydroperoxy substrate, initiated by small spatial adjustment of the exiting a-helix of loop IV.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Biel K.Y., Fomina I.R., Nazarova G.N., Soukhovolsky V.G., Khlebopros R.G., Nishio J.N.
Заглавие : Untangling metabolic and spatial interactions of stress tolerance in plants. 1. Patterns of carbon metabolism within leaves
Место публикации : Protoplasma. - 2010. - Vol. 245, Is. 1. - С. 49-73. - ISSN 0033183X (ISSN) , DOI 10.1007/s00709-010-0135-7
Ключевые слова (''Своб.индексиров.''): carbon metabolism--leaf anatomy--leaf form and function--maximal ecological utility--photosynthesis--stress tolerance spinacia oleracea--aspartate aminotransferase isoenzyme 1--bicarbonate--carbon--carbon dioxide--catalase--chlorophyll--malate dehydrogenase--oxygen--ribulosebisphosphate carboxylase--vegetable protein--article--enzymology--histology--light--metabolism--oxidation reduction reaction--photosynthesis--physiological stress--physiology--plant leaf--spinach--theoretical model--aspartate aminotransferase, cytoplasmic--bicarbonates--carbon--carbon dioxide--catalase--chlorophyll--light--malate dehydrogenase--models, theoretical--oxidation-reduction--oxygen--photosynthesis--plant leaves--plant proteins--ribulose-bisphosphate carboxylase--spinacia oleracea--stress, physiological--spinacia oleracea
Аннотация: The localization of the key photoreductive and oxidative processes and some stress-protective reactions within leaves of mesophytic C3 plants were investigated. The role of light in determining the profile of Rubisco, glutamate oxaloacetate transaminase, catalase, fumarase, and cytochrome-c-oxidase across spinach leaves was examined by exposing leaves to illumination on either the adaxial or abaxial leaf surfaces. Oxygen evolution in fresh paradermal leaf sections and CO2 gas exchange in whole leaves under adaxial or abaxial illumination was also examined. The results showed that the palisade mesophyll is responsible for the midday depression of photosynthesis in spinach leaves. The photosynthetic apparatus was more sensitive to the light environment than the respiratory apparatus. Additionally, examination of the paradermal leaf sections by optical microscopy allowed us to describe two new types of parenchyma in spinach-pirum mesophyll and pillow spongy mesophyll. A hypothesis that oxaloacetate may protect the upper leaf tissue from the destructive influence of active oxygen is presented. The application of mathematical modeling shows that the pattern of enzymatic distribution across leaves abides by the principle of maximal ecological utility. Light regulation of carbon metabolism across leaves is discussed. В© 2010 Springer-Verlag.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Degermendzhy A.G., Gulati R.D.
Заглавие : Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia)
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 331-340. - ISSN 13862588 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon budget--cyanobacteria--heterotrophic bacteria--hydrogen sulphide--mathematical models of stratification--meromictic lakes--microbial loop--stratification--trophic scheme--algal bloom--ecosystem modeling--limiting factor--nutrient availability--phytoplankton--saline lake--trophic interaction--russian federation--algae--bacteria (microorganisms)--cyanobacteria--lyngbya--lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovel E. S., Kicheeva A. G., Vnukova N. G., Churilov G. N., Stepin E. A., Kudryasheva N. S.
Заглавие : Toxicity and antioxidant activity of fullerenol c60,70 with low number of oxygen substituents
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2021. - Vol. 22, Is. 12. - Ст.6382. - ISSN 16616596 (ISSN), DOI 10.3390/ijms22126382
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovel, Ekaterina S., Kicheeva, Arina G., Vnukova, Natalia G., Churilov, Grigory N., Stepin, Evsei A., Kudryasheva, Nadezhda S.
Заглавие : Toxicity and Antioxidant Activity of Fullerenol C-60,C-70 with Low Number of Oxygen Substituents
Колич.характеристики :17 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [N18-29-19003]; RFBR, Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [N20-44-243001]; Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Fundamental Study 2020-2025 (Russian Federation)
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 12. - Ст.6382. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms22126382
Примечания : Cited References:93. - This research was funded by RFBR, N18-29-19003; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, N20-44-243001; and partly supported by the Program of the Federal Service for Surveillance on Consumer Rights Protection and HumanWellbeing, Fundamental Study 2020-2025 (Russian Federation).
Предметные рубрики: HUMIC SUBSTANCES
DETOXIFICATION PROCESSES
BIOLOGICAL-ACTIVITY
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene's carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A., Shikhov V.N., Gros J.-B., Golovko T.K., Dal'Ke I.V., Zakhozhii I.G.
Заглавие : Tolerance of wheat and lettuce plants grown on human mineralized waste to high temperature stress
Место публикации : Advances in Space Research. - 2013. - Vol. 51, Is. 11. - С. 2075-2083. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2013.01.017
Ключевые слова (''Своб.индексиров.''): air temperature step--blss--heat resistance--human waste recycling--lettuce--wheat--air temperature--blss--human waste--lettuce--wheat--atmospheric temperature--electromagnetic field effects--electromagnetic fields--gas plants--heat resistance--oxygen supply--plants (botany)--productivity--specific heat--thermal stress--waste utilization--plant shutdowns
Аннотация: The main objective of a life support system for space missions is to supply a crew with food, water and oxygen, and to eliminate their wastes. The ultimate goal is to achieve the highest degree of closure of the system using controlled processes offering a high level of reliability and flexibility. Enhancement of closure of a biological life support system (BLSS) that includes plants relies on increased regeneration of plant waste, and utilization of solid and liquid human wastes. Clearly, the robustness of a BLSS subjected to stress will be substantially determined by the robustness of the plant components of the phototrophic unit. The aim of the present work was to estimate the heat resistance of two plants (wheat and lettuce) grown on human wastes. Human exometabolites mineralized by hydrogen peroxide in an electromagnetic field were used to make a nutrient solution for the plants. We looked for a possible increase in the heat tolerance of the wheat plants using changes in photosynthetically active radiation (PAR) intensity during heat stress. At age 15 days, plants were subjected to a rise in air temperature (from 23 В± 1 В°C to 44 В± 1 В°) under different PAR intensities for 4 h. The status of the photosynthetic apparatus of the plants was assessed by external 2 gas exchange and fluorescence measurements. The increased irradiance of the plants during the high temperature period demonstrated its protective action for both the photosynthetic apparatus of the leaves and subsequent plant growth and development. The productivity of the plants subjected to temperature changes at 250 W m-2 of PAR did not differ from that of controls, whereas the productivity of the plants subjected to the same heat stress but in darkness was halved. В© 2012 COSPAR. Published by Elsevier Ltd.
Scopus
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Shikhov V.N., Gros J.-B., Golovko T.K., Dal'Ke I.V., Zakhozhii I.G.
Заглавие : Tolerance of plants grown on human mineralized waste to changes in air temperature
Место публикации : International Astronautical Federation - 59th International Astronautical Congress 2008, IAC 2008. - 2008. - Vol. 1: 59th International Astronautical Congress 2008, IAC 2008 (29 September 2008 through 3 October 2008, Glasgow) Conference code: 79748. - С. 311-320
Ключевые слова (''Своб.индексиров.''): air temperature--alternating electromagnetic field--biological life support systems--closedness--controlled process--fluorescence measurements--gas exchange--heat stress--high temperature--human waste--nutrient solution--old plants--photosynthetic apparatus--photosynthetically active radiation--plant components--plant growth--plant wastes--protective action--reproductive organs--temperature changes--temperature conditions--atmospheric temperature--electromagnetic field effects--electromagnetic fields--gas plants--heat resistance--hydrogen peroxide--oxygen supply--plant life extension--productivity--specific heat--thermal stress--waste utilization--plant shutdowns
Аннотация: The main objective of an LSS is to supply a crew with food, water and oxygen, and to eliminate its waste. The ultimate goal is to achieve the highest degree of closure of the system using controlled processes offering a high level of reliability and flexibility. Enhancement of closedness of biological life support systems (BLSS) including plants relies on increased regeneration of gas, water and plant waste, and utilization of solid and liquid human wastes. Clearly, the robustness of an LSS subjected to stress will be substantially determined by the robustness of the plant components of the phototrophic unit. The aim of the present work was to estimate the heat resistance of plants grown on human wastes. Human exometabolites mineralized by hydrogen peroxide in an alternating electromagnetic field were used to make a nutrient solution for the plants. We looked for a possible increase in the heat resistance of the plants using changes in photosynthetically active radiation (PAR) intensity during heat stress. At ages 15 and 25 days, plants were subjected to a rise in air temperature (from 22-24В°C to 44В°C) under different PAR intensities for 4 hours. The status of the photosynthetic apparatus of the plants was assessed by external CO2 gas exchange and fluorescence measurements. The increased irradiance of the plants during the high temperature period demonstrated its protective action for both the photosynthetic apparatus of the leaves and subsequent plant growth and development. The productivity of the plants subjected to temperature changes at 250 W/m2 of PAR did not differ from that of controls, whereas the productivity of the plants subjected to the same heat stress but in darkness was halved. The heat resistance of the reproductive organs of 25-day-old plants was significantly lower than that of 15-day-old plants subjected to similar light and temperature conditions.
Scopus
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zadereev E.S., Tolomeyev A.P., Drobotov A.V., Emeliyanova A.Y., Gubanov M.V.
Заглавие : The vertical distribution and abundance of Gammarus lacustris in the pelagic zone of the meromictic lakes Shira and Shunet (Khakassia, Russia)
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 531-539. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9329-5
Ключевые слова (''Своб.индексиров.''): amphipod--gammarus lacustris--meromictic lake--thermocline--vertical distribution--abundance--amphipod--dissolved oxygen--intertidal environment--meromictic lake--pelagic environment--seston--stratification--thermocline--vertical distribution--videography--water temperature--khakassia--lake shira--lake shunet--russian federation--amphipoda--animalia--gammarus--gammarus lacustris
Аннотация: The vertical distribution and abundance of Gammarus lacustris in the pelagic zone of two fishless meromictic lakes, L. Shira and L. Shunet, in Southern Siberia (Russia), was studied with the underwater video recording system and using vertical hauls. In both lakes, during summer stratification, Gammarus was distributed non-homogenously, with a stable peak in the metalimnion. The average depth of Gammarus population in the pelagic zone was significantly correlated with the depth of the thermocline. Gammarus abundances obtained using vertical plankton hauls with net were quite comparable with those obtained from video records. The peak abundance of Gammarus in the pelagic zone of the lakes observed with underwater video amounted up to 400 individuals m-2, while the peak animal densities in the metalimnion reached 50 ind. m-3. The data are compared with previously published abundances of Gammarus in the littoral of Lake Shira. Both littoral and pelagic can be equally important habitats for amphipods in meromictic lakes. The absence of fish in the pelagic zone, high oxygen concentration, low water temperature, increased seston concentration, elevated water density in the metalimnion and the anoxic hypolimnion can be the most probable combination of factors that are responsible for the peak of Gammarus in the metalimnion of these lakes. В© 2010 Springer Science+Business Media B.V.
Scopus
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nechipurenko Y. D., Semyonov D. A., Lavrinenko I. A., Lagutkin D. A., Generalov E. A., Zaitceva A. Y., Matveeva O. V., Yegorov Y. E.
Заглавие : The role of acidosis in the pathogenesis of severe forms of COVID-19
Место публикации : Biology: MDPI, 2021. - Vol. 10, Is. 9. - Ст.852. - ISSN 20797737 (ISSN), DOI 10.3390/biology10090852
Аннотация: COVID-19 has specific characteristics that distinguish this disease from many other infec-tions. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient’s condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregu-lation of the immune system and multidirectional pro-and anti-inflammatory reactions, resulting in the development of a “cytokine storm”. In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nechipurenko, Yury D., Semyonov, Denis A., Lavrinenko, Igor A., Lagutkin, Denis A., Generalov, Evgenii A., Zaitceva, Anna Y., Matveeva, Olga, V, Yegorov, Yegor E.
Заглавие : The Role of Acidosis in the Pathogenesis of Severe Forms of COVID-19
Колич.характеристики :15 с
Коллективы : Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]
Место публикации : Biology-Basel: MDPI, 2021. - Vol. 10, Is. 9. - Ст.852. - ISSN 2079-7737(eISSN), DOI 10.3390/biology10090852
Примечания : Cited References:86. - This research was funded by the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818.
Предметные рубрики: RESPIRATORY-ACIDOSIS
LACTATE
COAGULATION
GLYCOLYSIS
SECRETION
Аннотация: Simple Summary Recently, several studies have shown that acidosis, which is increased acidity in the blood and other body tissues, is often associated with severe COVID-19. In this article, we look at the mechanisms and consequences of acidosis that can lead to an exacerbation of COVID-19. We want to draw the attention of readers to the threshold values of such disease characteristics as hypoxia and acidosis, which are associated with a sharp deterioration in the patient's condition. Hypoxia and acidosis mutually reinforce each other according to the principle of a vicious cycle (that is, they are involved in a system of positive feedbacks). Elevated blood lactate (lactic acid) levels are associated with poor clinical outcomes in COVID patients. As a practical recommendation, we propose to pay more attention to the prevention of acidosis, including in the early stages of the disease, when the adjustment of homeostasis requires less effort and is less risky. COVID-19 has specific characteristics that distinguish this disease from many other infections. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient's condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregulation of the immune system and multidirectional pro- and anti-inflammatory reactions, resulting in the development of a "cytokine storm". In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shakirova, Anastasia A., Tomilin, Felix N., Pomogaev, Vladimir A., Vnukova, Natalia G., Churilov, Grigory N., Kudryasheva, Nadezhda S., Tchaikovskaya, Olga N., Ovchinnikov, Sergey G., Avramov, Pavel V.
Заглавие : Synthesis, Mass Spectroscopy Detection, and Density Functional Theory Investigations of the Gd Endohedral Complexes of C-82 Fullerenols
Колич.характеристики :12 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [18-29-19003 MK]; Russian Ministry of Science and EducationMinistry of Education and Science, Russian Federation [0721-2020-0033]; Collaborative NRF-RFBR grant (Korean) [NRF-2019K2A9A1A06100125]; Collaborative NRF-RFBR grant (Russian) [19-53-51005 NIFa RFFI-Korea]; NRF [2021R1A2C1010455]
Место публикации : Computation: MDPI, 2021. - Vol. 9, Is. 5. - Ст.58. - ISSN 2079-3197(eISSN), DOI 10.3390/computation9050058
Примечания : Cited References:41. - The experimental results were funded by RFBR project No. 18-29-19003 MK. The quantum chemical study was funded by project 0721-2020-0033 of the Russian Ministry of Science and Education. The collaboration and coordination of Russian and Korean teams was supported by Collaborative NRF-RFBR grant (Korean ID: NRF-2019K2A9A1A06100125; Russian ID: Project No. 19-53-51005 NIFa RFFI-Korea) and NRF 2021R1A2C1010455 grant.
Предметные рубрики: ZETA VALENCE QUALITY
BIOLOGICAL-ACTIVITY
BASIS-SETS
TOXICITY
Аннотация: Gd endohedral complexes of C-82 fullerenols were synthesized and mass spectrometry analysis of their composition was carried out. It was established that the synthesis yields a series of fullerenols Gd@C82Ox(OH)(y) (x = 0, 3; y = 8, 16, 24, 36, 44). The atomic and electronic structure and properties of the synthesized fullerenols were investigated using the density functional theory calculations. It was shown that the presence of endohedral gadolinium increases the reactivity of fullerenols. It is proposed that the high-spin endohedral fullerenols are promising candidates for application in magnetic resonance imaging.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Syromotina D. S., Surmenev R. A., Surmeneva M. A., Boyandin A. N., Nikolaeva E. D., Prymak O., Epple M., Ulbricht M., Oehr C., Volova T. G.
Заглавие : Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma
Место публикации : Mater. Sci. Eng. C. - 2016. - Vol. 62. - С. 450-457. - ISSN 09284931 (ISSN) , DOI 10.1016/j.msec.2016.01.075
Ключевые слова (''Своб.индексиров.''): plasma--polar component--poly-3-hydroxybutyrate membranes--surface energy--surface treatment--ammonia--biodegradable polymers--cell adhesion--contact angle--free energy--interfacial energy--plasma applications--plasmas--surface chemistry--surface properties--surface roughness--surface topography--wetting--biological performance--crystalline structure--polar components--poly-3-hydroxybutyrate--surface free energy--surface roughness changes--surface wettability--water contact angle--surface treatment
Аннотация: The surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer, with the exception being a power level of 250 W. The wettability of the modified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. It was revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma. © 2016 Elsevier B.V.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maillard-Schaller E., Kuettel O.M., Diederich L., Schlapbach L., Zhirnov V.V., Belobrov P.I.
Заглавие : Surface properties of nanodiamond films deposited by electrophoresis on Si(100)
Место публикации : Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - С. 805-808. - ISSN 09259635 (ISSN)
Ключевые слова (''Своб.индексиров.''): energy band diagram--nanodiamond--raman spectroscopy--surface characterization--band structure--electrodeposition--electrophoresis--hydrogen--nanostructured materials--nitrogen--oxidation--oxygen--phonons--plasma applications--silicon wafers--surface properties--dielectrophoresis--negative electron affinity (nea)--phonon confinement effect--diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Liu Z.J., Vysotski E.S., Chen C.J., Rose J.P., Lee J..., Wang B.C.
Заглавие : Structure of the Ca2+-regulated photoprotein obelin at 1.7 angstrom resolution determined directly from its sulfur substructure
Колич.характеристики :9 с
Место публикации : Protein Sci.: CAMBRIDGE UNIV PRESS, 2000. - Vol. 9, Is. 11. - С. 2085-2093. - ISSN 0961-8368
Примечания : Cited References: 41
Предметные рубрики: CALCIUM-MODULATED PROTEINS
AMINO-ACID SEQUENCE
CA-2+-BINDING PHOTOPROTEIN
CA2+-BINDING PHOTOPROTEIN
MACROMOLECULAR STRUCTURES
3-DIMENSIONAL STRUCTURE
ANOMALOUS SCATTERING
CRYSTAL-STRUCTURES
DIFFRACTION DATA
BINDING SITE
Ключевые слова (''Своб.индексиров.''): bioluminescence--crystallography--obelin--photoprotein--single wavelength anomalous scattering--solvent flattening--sulfur phasing
Аннотация: The crystal structure of the photoprotein obelin (22.2 kDa) from Obelia longissima has been determined and refined to 1.7 Angstrom resolution. Contrary to the prediction of a peroxide, the noncovalently bound substrate, coelenterazine, has only a single oxygen atom bound at the C2-position. The protein-coelenterazine 2-oxy complex observed in the crystals is photo-active because, in the presence of calcium ion, bioluminescence emission within the crystal is observed. This structure represents only the second de novo protein structure determined using the anomalous scattering signal of the sulfur substructure in the crystal. The method used here is theoretically different from that used for crambin in 1981 (4.72 kDa) and represents a significant advancement in protein crystal structure determination.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kobzeva T.V., Melnikov A.R., Karogodina T.Y., Zikirin S.B., Stass D.V., Molin Y.N., Rodicheva E.K., Medvedeva S.E., Puzyr A.P., Burov A.A., Bondar V.S., Gitelson J.I.
Заглавие : Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation
Колич.характеристики :8 с
Коллективы : Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29, Is. 7. - С. 703-710. - ISSN 1522-7235, DOI 10.1002/bio.2656. - ISSN 1522-7243
Примечания : Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058).
Предметные рубрики: BIOLUMINESCENCE
COMPONENTS
MECHANISMS
SYSTEM
Ключевые слова (''Своб.индексиров.''): higher luminous fungi--neonothopanus nambi--ionizing irradiation--reactive oxygen species--lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stepanyuk G.A., Liu Z.J., Burakova L.P., Lee J..., Rose J..., Vysotski E.S., Wang B.C.
Заглавие : Spatial structure of the novel light-sensitive photoprotein berovin from the ctenophore Beroe abyssicola in the Ca2+-loaded apoprotein conformation state
Колич.характеристики :8 с
Коллективы : RFBR [09-04-00172, 12-04-00131, 12-04-91153]; NSFC [31270795, 31021062]; Government of Russian Federation of the RAS [11.G34.31.0058]; National Institutes of Health [GM62407]; Georgia Research Alliance; University of Georgia Research Foundation; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [W-31-109-Eng-38]
Место публикации : BBA-Proteins Proteomics: ELSEVIER SCIENCE BV, 2013. - Vol. 1834, Is. 10. - С. 2139-2146. - ISSN 1570-9639, DOI 10.1016/j.bbapap.2013.07.006
Примечания : Cited References: 64. - This work was supported by RFBR grants 09-04-00172, 12-04-00131, 12-04-91153, and NSFC 31270795 and 31021062, by the Programs of the Government of Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.0058) "Molecular and Cellular Biology" of the RAS. It was also supported in part with funds from the National Institutes of Health (GM62407), The Georgia Research Alliance and the University of Georgia Research Foundation. Data were collected at Southeast Regional Collaborative Access Team (SER-CAT) 22-ID beamline at the Advanced Photon Source, Argonne National Laboratory. Supporting institutions may be found at www.ser-cat.org/members.html. The use of the Advanced Photon Source was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. W-31-109-Eng-38.
Предметные рубрики: CALCIUM-ACTIVATED PHOTOPROTEINS
COELENTERAZINE-BINDING PROTEIN
CRYSTAL-STRUCTURE
MNEMIOPSIS-SP
CA2+-REGULATED PHOTOPROTEINS
OBELIN BIOLUMINESCENCE
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
RENILLA-RENIFORMIS
APO-OBELIN
Ключевые слова (''Своб.индексиров.''): coelenterazine--calcium--bioluminescence--luciferase
Аннотация: The bright bioluminescence of ctenophores, found in oceans worldwide, is determined by Ca2+-regulated photoproteins, functionally identical to and sharing many properties of hydromedusan photoproteins. In contrast, however, the ctenophore photoproteins are extremely sensitive to UV and visible light over the range of their absorption spectrum. The spatial structure of a novel light-sensitive photoprotein from the ctenophore Beroe abyssicola in its apoform bound with three calcium ions is determined at 2.0 angstrom. We demonstrate that the apoberovin is a slightly asymmetrical compact globular protein formed by two domains with a cavity in the center, which exactly retains the fold architecture characteristic of hydromedusan photoproteins despite their low amino acid sequence identity. However, the structural alignment of these two photoprotein classes clearly shows that despite the high similarity of shape and geometry of their coelenterazine-binding cavities, their interiors differ drastically. The key residues appearing to be crucial for stabilizing the 2-hydroperoxycoelenterazine and for formation of the emitter in hydromedusan photoproteins, are replaced in berovin by amino acid residues having completely different side chain properties. Evidently, these replacements must be responsible for the distinct properties of ctenophore photoproteins such as sensitivity to light or the fact that the formation of active photoprotein from apophotoprotein, coelenterazine, and oxygen is more effective at alkaline pH. (C) 2013 Elsevier B.V. All rights reserved.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D.Y., Genova S.N., Gulati R.D., Degermendzhy A.G.
Заглавие : Some generalizations based on stratification and vertical mixing in meromictic Lake Shira, Russia, in the period 2002-2009
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 485-496. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9328-6
Ключевые слова (''Своб.индексиров.''): 1-d model--meromixis--mixolimnion--oxic-anoxic interface--thermocline--weather conditions--brackish water--meromictic lake--numerical model--one-dimensional modeling--overturn--physicochemical property--salinity--seasonality--stratification--temperate environment--vertical mixing--water temperature--weather--khakassia--lake shira--russian federation
Аннотация: In a brackish, temperate, 24-m-deep Lake Shira, the profiles of salinity, temperature, oxygen and sulfide concentrations were measured on a seasonal basis from 2002 to 2009. The lake was shown to be meromictic with autumnal overturn restricted to mixolimnion. The depth of mixolimnion and position of oxic-anoxic interface varied annually. The spring mixing processes contribute to the formation of mixolimnion in autumn. The exceptionally windy spring of 2007 caused the deepening of mixolimnion in the winter of 2008. The winter position of oxic-anoxic interface was affected by the position of lower boundary of mixolimnion in all winters. The salinity in the winter mixolimnion increased compared with the autumn because of freezing out of salts from the upper water layers meters during ice formation and their dissolution in water below. The profiles of salinity and temperature were simulated by the mathematical 1-D model of temperature and salinity conditions taking into account ice formation. The simulated profiles generally coincided with the measured ones. The coincidence implies that simplified one-dimensional model can be applied to roughly describe salinity and density profiles and mixing behavior of Lake Shira. В© 2010 The Author(s).
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lunina O.N., Bryantseva I.A., Akimov V.N., Rusanov I.I., Rogozin D.Yu., Barinova E.S., Lysenko A.M., Pimenov N.V.
Заглавие : Seasonal changes in the structure of the anoxygenic photosynthetic bacterial community in Lake Shunet, Khakassia
Место публикации : Microbiology. - 2007. - Vol. 76, Is. 3. - С. 368-379. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261707030149
Ключевые слова (''Своб.индексиров.''): anoxygenic photosynthesis--eutrophic meromictic saline lakes--green sulfur phototrophic bacteria--purple nonsulfur phototrophic bacteria--purple sulfur phototrophic bacteria--bacteria (microorganisms)--chlorobi--chromatiaceae--halochromatium--photobacteria--prosthecochloris vibrioformis--proteobacteria--rhodospirillaceae--rhodovulum--rhodovulum euryhalinum--thiocapsa
Аннотация: Seasonal studies of the anoxygenic phototrophic bacterial community of the water column of the saline eutrophic meromictic Lake Shunet (Khakassia) were performed in 2002 (June) and 2003 (February-March and August). From the redox zone down, the lake water was of dark green color. Green sulfur bacteria predominated in every season. The maximum number of green sulfur bacteria was 107 cells/ml in summer and 106 cells/ml in winter. A multi-syringe stratification sampler was applied for the study of the fine vertical distribution of phototrophs in August 2003; the sampling was performed every 5 cm. A 5-cm-thick pink-colored water layer inhabited by purple sulfur bacteria was shown to be located above the layer of green bacteria. The species composition and ratio of purple bacterial species depended on the sampling depth and on the season. In summer, the number of purple sulfur bacteria in the layer of pink water was 1.6 ? 108 cells/ml. Their number in winter was 3 ? 105 cells/ml. In the upper oxygen-containing layer of the chemocline the cells of purple nonsulfur bacteria were detected in summer. The maximum number of nonsulfur purple bacteria, 5 ? 102 cells/ml, was recorded in August 2003. According to the results of the phylogenetic analysis of pure cultures of the isolated phototrophic bacteria, which were based on 16S rDNA sequencing, green sulfur bacteria were close to Prosthecochloris vibrioformis, purple sulfur bacteria, to Thiocapsa and Halochromatium species, and purple nonsulfur bacteria, to Rhodovulum euryhalinum and Pinkicyclus mahoneyensis. В© Nauka/Interperiodica 2007.
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