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Accumulation of elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cav.) Trin. ex Steud.) macrophytes under different salinity levels / O. V. Anishchenko, A. P. Tolomeev, E. A. Ivanova [et al.] // Plant Physiol. Biochem. - 2020. - Vol. 154. - P328-340, DOI 10.1016/j.plaphy.2020.05.019 . - ISSN 0981-9428
Кл.слова (ненормированные):
Aquatic plants -- Essential/beneficial elements -- Non-essential elements -- Water conductivity
Аннотация: Accumulation of essential/beneficial and non-essential chemical elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cav.) Trin. ex Steud.) rooted macrophytes in three water bodies was studied as dependent on water salinity (specific conductivity) and main environmental factors (temperature, pH of water, growing period, element contents in water and sediments). Inductively coupled plasma optical emission spectrometry (ICP - OES) was used for the element detection in the plants, sediments, and water. Multivariate statistics redundancy analysis (RDA) showed that investigated factors together explained 37.8 - 44.7% and 29.9 - 42.6% of the essential/beneficial and non-essential elements variability, respectively, whereas salinity was one of the main factors determining the accumulation of a number of elements (Mg, S, Na, Li, Sr, Sb) by the macrophytes. A decrease in the essential element contents (K, P, N, Cu, Zn) was observed in both species of the macrophytes during the growing season. Environmental factors (salinity, growing period, and pH) were more significant than the element contents in water and the sediments for the essential/beneficial element accumulation by two species of the macrophytes. The element contents in the sediments to a greater extent determined non-essential element accumulation by P. australis than by S. pectinata. The study showed that the efficiency of using P. australis and S. pectinata for remediation of aquatic ecosystems contaminated by heavy metals should not decrease with a salinity increase. © 2020 Elsevier Masson SAS

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, 50/50, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79, Svobodny av., Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Anishchenko, O. V.; Tolomeev, A. P.; Ivanova, E. A.; Drobotov, A. V.; Kolmakova, A. A.; Zuev, I. V.; Gribovskaya, I. V.

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Accumulation of elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cay.) Trin. ex Steud.) macrophytes under different salinity levels / O. V. Anishchenko, A. P. Tolomeev, E. A. Ivanova [et al.] // Plant Physiol. Biochem. - 2020. - Vol. 154. - P328-340, DOI 10.1016/j.plaphy.2020.05.019. - Cited References:44. - The work was supported by Russian Foundation for Basic Research, grant No. 15-04-00797-a. The authors are grateful to the staff of Analytical Laboratory at the Institute of Biophysics SB RAS for the plant and sediment sample preparation for ICP-OES analysis and Elena Krasova for linguistic check and improvements. . - ISSN 0981-9428

РУБ Plant Sciences
Рубрики:
SEDIMENT QUALITY GUIDELINES
HEAVY-METAL ACCUMULATION
SALT-MARSH
Кл.слова (ненормированные):
Aquatic plants -- Essential/beneficial elements -- Non-essential elements -- Water conductivity
Аннотация: Accumulation of essential/beneficial and non-essential chemical elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cay.) Trin. ex Steud.) rooted macrophytes in three water bodies was studied as dependent on water salinity (specific conductivity) and main environmental factors (temperature, pH of water, growing period, element contents in water and sediments). Inductively coupled plasma optical emission spectrometry (ICP - OES) was used for the element detection in the plants, sediments, and water. Multivariate statistics redundancy analysis (RDA) showed that investigated factors together explained 37.8 44.7% and 29.9 - 42.6% of the essential/beneficial and non-essential elements variability, respectively, whereas salinity was one of the main factors determining the accumulation of a number of elements (Mg, S, Na, Li, Sr, Sb) by the macrophytes. A decrease in the essential element contents (K, P, N, Cu, Zn) was observed in both species of the macrophytes during the growing season. Environmental factors (salinity, growing period, and pH) were more significant than the element contents in water and the sediments for the essential/beneficial element accumulation by two species of the macrophytes. The element contents in the sediments to a greater extent determined non-essential element accumulation by P. australis than by S. pectinata. The study showed that the efficiency of using P. australis and S. pectinata for remediation of aquatic ecosystems contaminated by heavy metals should not decrease with a salinity increase.

WOS
Держатели документа:
Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Akad Gorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Av, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Anishchenko, O., V; Tolomeev, A. P.; Ivanova, E. A.; Drobotov, A., V; Kolmakova, A. A.; Zuev, I., V; Gribovskaya, I., V; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [15-04-00797-a]

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Assessment of the rate of accumulation of the transuranium element americium-241 by the aquatic plant Elodea canadensis / A. Ya. Bolsunovskii [et al.] // Doklady Biological Sciences. - 2004. - Vol. 399, Is. 1-6. - P467-469, DOI 10.1007/s10630-005-0014-0 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- radioisotope -- water -- article -- biomass -- drug effect -- environmental monitoring -- Hydrocharitaceae -- methodology -- pH -- plant -- plant root -- radiation exposure -- radiochemistry -- time -- toxicity testing -- water pollutant -- Americium -- Biomass -- Environmental Monitoring -- Hydrocharitaceae -- Hydrogen-Ion Concentration -- Plant Roots -- Plant Shoots -- Plants -- Radiochemistry -- Radioisotopes -- Time Factors -- Toxicity Tests -- Water -- Water Pollutants, Radioactive -- Elodea canadensis

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

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^a341.47.51.15.19.13^2VINITI
A99

Aдсорбция афлатоксина В1 наноалмазами детонационного синтеза [Текст] : научное издание / А. П. Пузырь, К. В. Пуртов, О. А. Шендерова [и др.] // Докл. РАН. - 2007. - Т. 417, N 1. - С. 117-120 . - ISSN 0869-5652

ГРНТИ

34.47.51

РУБ 341.47.51.15.19.13
Рубрики:
ПИЩЕВЫЕ ПРОДУКТЫ
   КОРМА
   ЗАГРЯЗНЕНИЕ МИКОТОКСИНАМИ
   АФЛАТОКСИНЫ
   УДАЛЕНИЕ
   АДСОРБЕНТЫ
   ЭНТЕРОСОРБЕНТЫ
   НАНОАЛМАЗЫ
   ДЕТОНАЦИОННОГО СИНТЕЗА
Аннотация: Загрязнение кормов и продуктов питания токсинами микроскопических грибов, в частности афлатоксинами, представляет опасность для здоровья животных и человека. В качестве адсорбента для нейтрализации микотоксинов представляют интерес наноалмазы (НА) детонационного синтеза. Высокоразвитая поверхность НА, наличие на поверхности наночастиц различных химических активных функциональных групп, углеводородных фрагментов и микропримесей металлов определяют их высокие сорбционные свойства к биомолекулам. Установлена способность детонационных МНА адсорбировать АфB1 из водных растворов при кислых, слабокислых и щелочных значениях pH, причем время адсорбции микотоксина наночастицами не превышает 2-3 мин. Показано, что сорбционная емкость МНА к АфB1 зависит от соотношения количеств микотоксина и сорбента. В целом, полученные данные позволяют прогнозировать возможность практического применения данного наноматериала для энтеросорбции и нейтрализации микотоксинов, что является предметом дальнейших исследований. Россия, Ин-т биофизики СО РАН, Красноярск. Библ. 15

Доп.точки доступа:
Пузырь, А.П.; Пуртов, К.В.; Шендерова, О.А.; Луо, М.; Бреннер, Д.В.; Бондарь, В.С.

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Bacterial Communities of Three Saline Meromictic Lakes in Central Asia [Text] / B. Baatar [et al.] // PLoS One. - 2016. - Vol. 11, Is. 3. - Ст. e0150847, DOI 10.1371/journal.pone.0150847. - Cited References:65. - This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99- 2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a.; We thank our Russian and Mongolian colleagues in the Institute of Biophysics SB RAS and National University of Mongolia for assistance with providing samples and hydro-parameter data. This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99-2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
PURPLE SULFUR BACTERIA
REAL-TIME PCR
MICROBIAL COMMUNITIES
Аннотация: Meromictic lakes located in landlocked steppes of central Asia (similar to 2500 km inland) have unique geophysiochemical characteristics compared to other meromictic lakes. To characterize their bacteria and elucidate relationships between those bacteria and surrounding environments, water samples were collected from three saline meromictic lakes (Lakes Shira, Shunet and Oigon) in the border between Siberia and the West Mongolia, near the center of Asia. Based on in-depth tag pyrosequencing, bacterial communities were highly variable and dissimilar among lakes and between oxic and anoxic layers within individual lakes. Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria and Firmicutes were the most abundant phyla, whereas three genera of purple sulfur bacteria (a novel genus, Thiocapsa and Halochromatium) were predominant bacterial components in the anoxic layer of Lake Shira (similar to 20.6% of relative abundance), Lake Shunet (similar to 27.1%) and Lake Oigon (similar to 9.25%), respectively. However, few known green sulfur bacteria were detected. Notably, 3.94% of all sequencing reads were classified into 19 candidate divisions, which was especially high (23.12%) in the anoxic layer of Lake Shunet. Furthermore, several hydro-parameters (temperature, pH, dissolved oxygen, H2S and salinity) were associated (P0.05) with variations in dominant bacterial groups. In conclusion, based on highly variable bacterial composition in water layers or lakes, we inferred that the meromictic ecosystem was characterized by high diversity and heterogenous niches.

WOS,
Scopus
Держатели документа:
Acad Sinica, Taiwan Int Grad Program, Mol & Biol Agr Sci Program, Taipei 115, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan.
Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan.
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia.
Natl Pingtung Univ Sci & Technol, Pingtung 91201, Taiwan.
Natl Univ Mongolia, Sch Art & Sci, Ulaanbaatar 14201, Mongol Peo Rep.
Natl Chung Hsing Univ, Ctr Biotechnol, Taichung 40227, Taiwan.

Доп.точки доступа:
Baatar, Bayanmunkh; Chiang, Pei-Wen; Rogozin, Denis Yu; Wu, Yu-Ting; Tseng, Ching-Hung; Yang, Cheng-Yu; Chiu, Hsiu-Hui; Oyuntsetseg, Bolormaa; Degermendzhy, Andrey G.; Tang, Sen-Lin; National Sciences Council of Taiwan [NSC99- 2923-B-001-001-MY3, NSC 102-2923-B-001-004, NSC101-2923-B-001-003-MY3, NSC99-2923-B-001-001-MY3]; Biodiversity Research Center, Academia Sinica, Taiwan; Russian Foundation for Basic Research [14-04-01060-a]

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CADMIUM-INDUCED LUMINESCENCE OF RECOMBINANT PHOTOPROTEIN OBELIN [Text] / V. S. BONDAR [et al.] // Biochim. Biophys. Acta-Bioenerg. - 1995. - Vol. 1231, Is. 1. - P29-32, DOI 10.1016/0005-2728(95)00059-R. - Cited References: 21 . - ISSN 0005-2728

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
AEQUORIN
Кл.слова (ненормированные):
PHOTOPROTEIN -- OBELIN -- CADMIUM -- BIOLUMINESCENCE
Аннотация: It has been shown for the first time that Cd2+ ions induce substantial bioluminescence of a Ca2+-binding photoprotein: recombinant obelin. The optimum pH for the bioluminescent rr:action in the presence of Cd2+ ions is pH 6. The intensity, L, of the light emission for the Cd2+ ions is 75% of the intensity of the signal in the presence of Ca2+. The quantum yields of the reactions in the presence of Cd2+ and Ca2+ are 0.18 and 0.24 respectively. The slope of the straight line (between 5 and 90% of L,,) in the coordinates of log(L/(L(max) - L)) vs. log([Cd2+]) is 1.75 +/- 0.06, which indicates positive cooperative character of this reaction. At a concentration exceeding 1 . 10(-3) M, Cd2+ inhibits the bioluminescent reaction.

Держатели документа:
AGR UNIV WAGENINGEN,DEPT BIOCHEM,6703 HA WAGENINGEN,NETHERLANDS
RUSSIAN ACAD SCI,INST BIOPHYS,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
BONDAR, V.S.; SERGEEV, A.G.; ILLARIONOV, B.A.; VERVOORT, J...; HAGEN, W.R.

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Comparative study of temperature effects on bacterial luciferases [Text] / N. A. Tyulkova, T. P. Sandalova // Biochem.-Moscow. - 1996. - Vol. 61, Is. 2. - P. 205-214. - Cited References: 23 . - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
BIOLUMINESCENCE
Кл.слова (ненормированные):
bacterial luciferase -- temperature -- activation energy
Аннотация: Effects of temperature on bioluminescent patterns of luciferases from luminescent bacteria Vibrio harveyi, Vibrio fischeri, Photobacterium leiognathi, and Photobacterium phosphoreum were studied. The highest luminescence level was observed at 15-25 degrees C for the luciferase from P. phosphoreum, at 20-30 degrees C for the V. fischeri and P. leiognathi enzymes, and at 30-37 degrees C for the enzyme from V. harveyi. All the luciferases were significantly stabilized at increased salt concentrations, at low pH values, or in the presence of dithiothreitol (DTT) and EDTA. The addition of DTT and EDTA affected the reversible stage of enzyme inactivation, while salts reduced the rate of the irreversible stage. A peak corresponding to aggregated protein was detected by gel chromatography of irreversibly inactivated luciferase. Activation energies were calculated for each luciferase in bioluminescent reactions with decanal, dodecanal, tetradecanal, and without aldehydes. The activation energy of the reaction with tetradecanal was much lower than those with the other aldehydes. The temperature dependence of the lifetime of the long-lived reaction intermediate showed that in the 10-30 degrees C interval all the luciferases, except for the enzyme from V. harveyi, have only one active form.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tyulkova, N.A.; Sandalova, T.P.

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Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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Designing a reusable system based on nanodiamonds for biochemical determination of urea / N. O. Ronzhin [et al.] // Doklad. Biochem. Biophys. - 2015. - Vol. 465, Is. 1. - P428-431, DOI 10.1134/S1607672915060216 . - ISSN 1607-6729
Аннотация: A reusable system including urease covalently bound to the surface of modified nanodiamonds (MNDs) has been developed for the multiple determination of urea. The immobilized enzyme exhibits functional activity and catalyzes the hydrolysis of urea to yield ammonia. The presence of ammonia is confirmed by the formation of a colored product after the addition of chemical reagents. It was shown that the MNDs-urease complex can function in a wide range of temperatures and pH as well as in deionized water. The complex provides a linear yield of the product at low analyte concentrations and allows the multiple determination of urea in vitro. © 2015, Pleiades Publishing, Ltd.

Scopus,
WOS
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ronzhin, N. O.; Baron, A. V.; Bondar, V. S.; Gitelson, I. I.

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10.

Ecological features of cyanobacteria and algae communities of the littoral of the meromictic Lake Shira (Khakassia Republic, Russia) / L. A. Gaysina, A. I. Fazlutdinova, N. Mukhina [et al.] // Theor. Appl. Ecol. - 2020. - Is. 1. - P47-50, DOI 10.25750/1995-4301-2020-1-047-050. - Cited References:18. - The study was supported by Russian Foundation for Basic Research in the frame of project 19-05-00428 and an internal grant of M. Akmullah Bashkir State Pedagogical University for the year 2019. This article is published with financial support from the RFBR grant No. 19-04-20031. . - ISSN 1995-4301. - ISSN 2618-8406

РУБ Ecology

Кл.слова (ненормированные):
meromixia -- Bacillariophyta -- alkaliphilic species -- Leptolyngbya -- voronichiniana -- Pseudophormidium pauciramosum -- Desmodesmus abundans -- Oocystis lacustris -- Amphora ovalis -- Grunowia tabellaria -- Surirella -- undulata
Аннотация: During the study of cyanobacteria and algae from littoral of the meromictic Lake Shira (Republic of Khakassia, Russia) forty-eight taxa were identified: Cyanobacteria - 7, Chlorophyta - 5, and Bacillariophyta - 36 species. Cyanobacteria were represented by cosmopolitan taxa Leptolyngbya voronichiniana, cf. Trichocoleus hospitus and widely distributed in the freshwater ecosystems species Phormidium cf. paulsenianum, Pseudophormidium cf. golenkinianum, P. pauciramosum, Leptolyngbya perforans, and L. subtilissima. Among green algae typical freshwater taxa Chloroidium saccharophilum, Desmodesmus abundans, Oocystis lacustris, Chlorella sp., Stigeoclonium sp. were detected. Among Bacillariophyta the most frequent species were Amphora ovalis, Cymbella affinis, Encyonema silesiacum, Eunotia fallax, Gomphonella olivacea, G. parvulum, Kobayasiella subtilissima, Navicula minima, N. radiosa, N. veneta, Nitzchia fonticola, N. palea, Grunowia tabellaria, Surirella undulata. Several rare species cf. Fragilaria tenera, Grunowia tabellaria and cf. Ulnaria capitata were found. In relation to mineralization indifferents was the largest group (73%). In relation to pH, a unique feature of the investigated lake was the predominance of alkaliphilic species of diatoms (56%), such as Amphora ovalis, Cymbella affinis, Gomphonema truncatum, Hantzschia amphioxys, Navicula minima, N. veneta, Nitzschia cf. linearis, N. palea, Planothidium lanceolatum and other. In the geographic structure cosmopolitan group included 25 species (69%), boreal group - 4 species (11%), arcto-apline group - 2 species (6%). To obtain more accurate information on the biodiversity of cyanobacteria and algae of Lake Shira further studies using molecular-genetic and electron microscopic methods are needed.

WOS
Держатели документа:
M Akmullah Bashkir State Pedag Univ, 3-A Oktyabrskoy Revolucii, Ufa 450008, Republic Of Bas, Russia.
All Russian Res Inst Phytopathol, 5 Inst St, Odintsovo Dist 143050, Moscow Region, Russia.
RAS, Inst Biophys, Siberian Branch, 50 Akad Gorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Prospekt, Krasnoyarsk 660041, Russia.
John Carroll Univ, John Carroll Blvd, University Hts, OH 41118 USA.

Доп.точки доступа:
Gaysina, L. A.; Fazlutdinova, A., I; Mukhina, N.; Akhrnadeyeva, L. E.; Rogozin, D. Yu; Bul'khin, A. O.; Sulehanova, N., V; Johansen, J. R.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-05-00428]; internal grant of M. Akmullah Bashkir State Pedagogical University; RFBRRussian Foundation for Basic Research (RFBR) [19-04-20031]

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11.

Ecological features of cyanobacteria and algae communities of the littoral of the meromictic Lake Shira (Khakassia Republic, Russia) / L. A. Gaysina, A. I. Fazlutdinova, O. N. Mukhina [et al.] // Theor. Appl. Ecol. - 2020. - Vol. 2020, Is. 1. - P47-50, DOI 10.25750/1995-4301-2020-1-047-050 . - ISSN 1995-4301
Кл.слова (ненормированные):
Alkaliphilic species -- Amphora ovalis -- Bacillariophyta -- Desmodesmus abundans -- Grunowia tabellaria -- Leptolyngbya voronichiniana -- Meromixia -- Oocystis lacustris -- Pseudophormidium pauciramosum -- Surirella undulata
Аннотация: During the study of cyanobacteria and algae from littoral of the meromictic Lake Shira (Republic of Khakassia, Russia) forty-eight taxa were identified: Cyanobacteria - 7, Chlorophyta - 5, and Bacillariophyta - 36 species. Cyanobacteria were represented by cosmopolitan taxa Leptolyngbya voronichiniana, cf. Trichocoleus hospitus and widely distributed in the freshwater ecosystems species Phormidium cf. paulsenianum, Pseudophormidium cf. golenkinianum, P. pauciramosum, Leptolyngbya perforans, and L. subtilissima. Among green algae typical freshwater taxa Chloroidium saccharophilum, Desmodesmus abundans, Oocystis lacustris, Chlorella sp., Stigeoclonium sp. were detected. Among Bacillariophyta the most frequent species were Amphora ovalis, Cymbella affinis, Encyonema silesiacum, Eunotia fallax, Gomphonella olivacea, G. parvulum, Kobayasiella subtilissima, Navicula minima, N. radiosa, N. veneta, Nitzchia fonticola, N. palea, Grunowia tabellaria, Surirella undulata. Several rare species cf. Fragilaria tenera, Grunowia tabellaria and cf. Ulnaria capitata were found. In relation to mineralization indifferents was the largest group (73%). In relation to pH, a unique feature of the investigated lake was the predominance of alkaliphilic species of diatoms (56%), such as Amphora ovalis, Cymbella affinis, Gomphonema truncatum, Hantzschia amphioxys, Navicula minima, N. veneta, Nitzschia cf. linearis, N. palea, Planothidium lanceolatum and other. In the geographic structure cosmopolitan group included 25 species (69%), boreal group - 4 species (11%), arcto-apline group - 2 species (6%). To obtain more accurate information on the biodiversity of cyanobacteria and algae of Lake Shira further studies using molecular-genetic and electron microscopic methods are needed. © 2020 Publishing House "O- Kratkoe". All rights reserved.

Scopus
Держатели документа:
M. Akmullah Bashkir State Pedagogical University, 3-a, Okt'yabrskoy revolucii, Ufa, 450008, Russian Federation
All-Russian Research Institute of Phytopathology, 5, Institute St., B. Vyazyomy, Odintsovo District, Moscow Region, 143050, Russian Federation
Institute of Biophysics Siberian Branch of RAS, 50, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79, Svobodny Prospekt, Krasnoyarsk, 660041, Russian Federation
John Carroll University, University Heights, John Carroll Blvd., Ohio, 44118, United States

Доп.точки доступа:
Gaysina, L. A.; Fazlutdinova, A. I.; Mukhina, O. N.; Akhmadeyeva, L. F.; Rogozin, D. Y.U.; Bul'Khin, A. O.; Sukhanova, N. V.; Johansen, J. R.

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12.

EFFECT OF INHIBITING SODIUM-CHLORIDE CONCENTRATIONS ON THE GROWTH OF CANDIDA-TROPICALIS UNDER CONTINUOUS PH-STAT CULTURING CONDITIONS [Text] / A. V. FURYAEVA [et al.] // Microbiology. - 1985. - Vol. 54, Is. 6. - P708-713. - Cited References: 18 . - ISSN 0026-2617

РУБ Microbiology

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
FURYAEVA, A.V.; SAUBENOVA, M.G.; PUZYREVSKAYA, O.M.; MUKHAMEDIEVA, U.S.; TUSHKOVA, G.I.; PISMAN, T.I.; PECHURKIN, N.S.

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13.

Effect of the intracellular poly-?-hydroxybutyrate pool on the growth of a hydrogen-oxidizing bacterium under nonoptimum conditions / T. G. Volova, G. S. Kalacheva, A. P. Puzyr // Mikrobiologiya. - 1996. - Vol. 65, Is. 5. - P594-598 . - ISSN 0026-3656
Аннотация: -Growth of the hydrogen-oxidizing bacterium Alcaligenes eutrophus under nonoptimum conditions (acidic and alkaline pH, elevated and lowered temperatures, absence of exogenous sources of carbon and energy) was studied in relation to the initial intracellular concentration of poly-?-hydroxybutyrate (PHB), which varied from virtually zero, to 60-70% of the dry biomass. An initial PHB pool of greater than 30-40% provided for a 2- to 2.5-fold higher biomass yield in acidic and alkaline media or at nonoptimum temperatures as compared with cultures initially containing no pommer. The intracellular PHB pool also provides for the growth of the culture in the absence of exogenous carbon sources and its survival (without growth) with no exogenous energy supply.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Puzyr, A.P.

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14.

Effect of the intracellular poly-beta-hydroxybutyrate pool on the growth of a hydrogen-oxidizing bacterium under nonoptimum conditions [Text] / T. G. Volova, G. S. Kalacheva, A. P. Puzyr // Microbiology. - 1996. - Vol. 65, Is. 5. - P521-524. - Cited References: 16 . - 4. - ISSN 0026-2617

РУБ Microbiology
Рубрики:
MEMBRANES
Аннотация: Growth of the hydrogen-oxidizing bacterium Alcaligenes eutrophus under nonoptimum conditions (acidic and alkaline pH, elevated and lowered temperatures, absence of exogenous sources of carbon and energy) was studied in relation to the initial intracellular concentration of poly-beta-hydroxybutyrate (PHB), which varied from virtually zero to 60-70% of the dry biomass. An initial PHB pool of greater than 30-40% provided for a 2- to 2.5-fold higher biomass yield in acidic and alkaline media or at nonoptimum temperatures as compared with cultures initially containing no polymer. The intracellular PHB pool also provides for the growth of the culture in the absence of exogenous carbon sources and its survival (without growth) with no exogenous energy supply.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Puzyr, A.P.

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15.

Electrosynthesis of hydrogen peroxide from oxygen in a gas-diffusion electrode in solutions of mineralized exometabolites / G. A. Kolyagin [et al.] // Russian Journal of Electrochemistry. - 2013. - Vol. 49, Is. 10. - P1004-1007, DOI 10.1134/S1023193513100091 . - ISSN 1023-1935
Кл.слова (ненормированные):
electroreduction of oxygen -- electrosynthesis of hydrogen peroxide -- gas diffusion electrode -- life-support system -- mineralization of biowaste -- processing of exometabolites
Аннотация: The electrosynthesis of H2O2 in aqueous solutions (pH 1-9) of mineralized liquid and solid bio-wastes (exometabolites) for their processing in closed life-support systems was studied. It was shown that H 2O2 can be obtained in these solutions by electrochemical reduction of oxygen in carbon black gas diffuse electrodes to concentrations of over 2 M with current efficiency 80%. The composition of the solution was found to affect the accumulation of H2O2 during the synthesis. The solutions can be concentrated further to 19 M H2O2. The results showed that the electrolytic method is promising for preparing H2O2 for closed life-support systems. В© 2013 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kolyagin, G.A.; Kornienko, V.L.; Kudenko, Y.A.; Tikhomirov, A.A.; Trifonov, S.V.

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16.

Experimental approach to study the effect of mutations on the protein folding pathway / E. V. Nemtseva [et al.] // PLoS One. - 2019. - Vol. 14, Is. 1. - Ст. e0210361, DOI 10.1371/journal.pone.0210361. - Cited References:38. - The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Projects 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Project 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
FLUORESCENCE LIFETIMES ORIGIN
   TRANSITION-STATE
   EXCHANGE
   TRYPTOPHAN
Аннотация: Is it possible to compare the physicochemical properties of a wild-type protein and its mutant form under the same conditions? Provided the mutation has destabilized the protein, it may be more correct to compare the mutant protein under native conditions to the wild-type protein destabilized with a small amount of the denaturant. In general, is it appropriate to compare the properties of proteins destabilized by different treatments: mutations, pH, temperature, and denaturants like urea? These issues have compelled us to search for methods and ways of presentation of experimental results that would allow a comparison of mutant forms of proteins under different conditions and lead to conclusions on the effect of mutations on the protein folding/unfolding pathway. We have studied equilibrium unfolding of wild-type bovine carbonic anhydrase II (BCA II) and its six mutant forms using different urea concentrations. BCA II has been already studied in detail and is a good model object for validating new techniques. In this case, time-resolved fluorescence spectroscopy was chosen as the basic research method. The main features of this experimental method allowed us to compare different stages of unfolding of studied proteins and prove experimentally that a single substitution of the amino acid in three mutant forms of BCA II affected the native state of the protein but did not change its unfolding pathway. On the contrary, the inserted disulfide bridge in three other mutant forms of BCA II affected the protein unfolding pathway. An important result of this research is that we have validated the new approach allowing investigation of the effect of mutations on the folding of globular proteins, because in this way it is possible to compare proteins in the same structural states rather than under identical conditions.

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Scopus
Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Prot Res, Pushchino, Moscow Region, Russia.

Доп.точки доступа:
Nemtseva, Elena V.; Gerasimova, Marina A.; Melnik, Tatiana N.; Melnik, Bogdan S.; Gerasimova, Marina; Nemtseva, Elena; Ministry of Science and Education of the Russian Federation [6.7734.2017]; Russian Science Foundation [N14-24-00157]

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17.

Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications / O. Mogilnaya, N. Ronzhin, E. Posokhina, V. Bondar // Protein J. - 2021, DOI 10.1007/s10930-021-10010-z. - Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, Project No. 0356-2019-0022. . - Article in press. - ISSN 1572-3887. - ISSN 1573-4943

РУБ Biochemistry & Molecular Biology
Рубрики:
ARYL-ALCOHOL OXIDASE
   GLUCOSE-OXIDASE
   PEROXIDASES
   MECHANISM
Кл.слова (ненормированные):
Extracellular oxidase -- Flavoprotein -- Fungi -- Phenol
Аннотация: The extracellular enzyme with oxidase function was extracted from the Neonothopanus nambi luminescent fungus by using mild processing of mycelium with beta-glucosidase and then isolated by gel-filtration chromatography. The extracted enzyme is found to be a FAD-containing protein, catalyzing phenol co-oxidation with 4-aminoantipyrine without addition of H2O2, which distinguishes it from peroxidases. This fact allowed us to assume that this enzyme may be a mixed-function oxidase. According to gel-filtration chromatography and SDS-PAGE, the oxidase has molecular weight of 60 kDa. The enzyme exhibits maximum activity at 55-70 degrees C and pH 5.0. Kinetic parameters K-m and V-max of the oxidase for phenol were 0.21 mM and 0.40 mu M min(-1). We suggest that the extracted enzyme can be useful to develop a simplified biosensor for colorimetric detection of phenol in aqueous media, which does not require using hydrogen peroxide.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Mogilnaya, Olga; Ronzhin, Nikita; Posokhina, Ekaterina; Bondar, Vladimir; [0356-2019-0022]

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18.

Extracellular Oxidases of Basidiomycete Neonothopanus nambi: Isolation and Some Properties / N. O. Ronzhin, O. A. Mogilnaya, K. S. Artemenko [et al.] // Dokl. Biochem. Biophys. - 2020. - Vol. 490, Is. 1. - P38-42, DOI 10.1134/S1607672920010135. - Cited References:15 . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
PEROXIDASE-ACTIVITY
LIGHT-EMISSION
Кл.слова (ненормированные):
extracellular oxidases -- basidiomycete Neonothopanus nambi -- beta-glucosidase -- gel-filtration chromatography -- veratryl alcohol -- phenol -- FAD
Аннотация: Using the original technique of treating biomass with beta-glucosidase, a pool of extracellular fungal enzymes was obtained for the first time from the mycelium of basidiomycete Neonothopanus nambi. Two protein fractions containing enzymes with oxidase activity were isolated from the extract by gel-filtration chromatography and conventionally called F1 and F2. Enzyme F1 has a native molecular weight of 80-85 kDa and does not contain chromophore components; however, it catalyzes the oxidation of veratryl alcohol with K-m = 0.52 mM. Probably, this enzyme is an alcohol oxidase. Enzyme F2 with a native molecular weight of approximately 60 kDa is a FAD-containing protein. It catalyzes the cooxidation of phenol with 4-aminoantipyrine without the addition of exogenous hydrogen peroxide, which distinguishes it from the known peroxidases. It was assumed that this enzyme may be a mixed-function oxidase. F2 oxidase has K-m value 0.27 mM for phenol. The temperature optimums for oxidases F1 and F2 are 22-35 and 55-70 degrees C, and pH optimums are 6 and 5, respectively.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci, Inst Biophys,Fed Res Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Ronzhin, N. O.; Mogilnaya, O. A.; Artemenko, K. S.; Posokhina, E. D.; Bondar, V. S.

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19.

Gelatin and starch as stabilizers of the coupled enzyme system of luminous bacteria NADH:FMN-oxidoreductase-luciferase / A. Bezrukikh [et al.] // . - 2014, DOI 10.1007/s00216-014-7987-1 . - ISSN 1618-2642
Кл.слова (ненормированные):
Bacterial luciferase -- Bioluminescence -- Gelatin -- NADH:FMN-oxidoreductase -- Stabilization of enzymes -- Starch
Аннотация: We have studied the effects of a gel-like environment on the characteristics of enzyme preparations based on the coupled enzyme system of luminous bacteria, NADH:FMN-oxidoreductase-luciferase, to design a stable immobilizing reagent for bioluminescent analysis. Natural polymers, gelatin and starch, were used to create a viscous, structured microenvironment. The stability of the coupled enzyme system to such physical and chemical environmental factors as temperature, pH, and ionic strength in gelatin and starch-containing media was examined. It was shown that both gelatin and starch have a stabilizing effect on the enzymes of luminous bacteria under specific conditions. In particular, the enzymes' activity is increased twofold in the presence of 1 and 5 % of gelatin at 20 °C and 25 °C, respectively (temperatures lower than the gel point). Also, the acceptable pH range of the coupled enzyme system expands into the alkaline region and becomes 6.8-8.1. Stabilization at low ionic strength (0.01-0.06 mol L-1) is observed. At the same time, microenvironments based on either gelatin or starch do not change the enzymes' thermal inactivation rate constants in the temperature range from 25 to 43 °C. Finally, gelatin and starch are suitable for development of a reagent for immobilization of enzymes which would be stable and resistant to physical and chemical environmental conditions. © 2014 Springer-Verlag Berlin Heidelberg.

Scopus
Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, pr. Svobodnyi 79, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bezrukikh, A.; Esimbekova, E.; Nemtseva, E.; Kratasyuk, V.; Shimomura, O.

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20.

Gelatin and starch as stabilizers of the coupled enzyme system of luminous bacteria NADH:FMN-oxidoreductase-luciferase [Text] / A. . Bezrukikh [et al.] // Anal. Bioanal. Chem. - 2014. - Vol. 406, Is. 23. - P5743-5747, DOI 10.1007/s00216-014-7987-1. - Cited References: 14. - The work was supported by the Program of the Government of Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant no. 11.G34.31.0058), the Russian Academy of Sciences (program "Molecular and Cell Biology", grant no. 6.8), and the state contract between the Ministry of Education and Science and Siberian Federal University, no. 1762. . - ISSN 1618-2642. - ISSN 1618-2650

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
IMMOBILIZATION
CHEMISTRY
Кл.слова (ненормированные):
Bacterial luciferase -- NADH:FMN-oxidoreductase -- Bioluminescence -- Stabilization of enzymes -- Gelatin -- Starch
Аннотация: We have studied the effects of a gel-like environment on the characteristics of enzyme preparations based on the coupled enzyme system of luminous bacteria, NADH:FMN-oxidoreductase-luciferase, to design a stable immobilizing reagent for bioluminescent analysis. Natural polymers, gelatin and starch, were used to create a viscous, structured microenvironment. The stability of the coupled enzyme system to such physical and chemical environmental factors as temperature, pH, and ionic strength in gelatin and starch-containing media was examined. It was shown that both gelatin and starch have a stabilizing effect on the enzymes of luminous bacteria under specific conditions. In particular, the enzymes' activity is increased twofold in the presence of 1 and 5 % of gelatin at 20 A degrees C and 25 A degrees C, respectively (temperatures lower than the gel point). Also, the acceptable pH range of the coupled enzyme system expands into the alkaline region and becomes 6.8-8.1. Stabilization at low ionic strength (0.01-0.06 mol L-1) is observed. At the same time, microenvironments based on either gelatin or starch do not change the enzymes' thermal inactivation rate constants in the temperature range from 25 to 43 A degrees C. Finally, gelatin and starch are suitable for development of a reagent for immobilization of enzymes which would be stable and resistant to physical and chemical environmental conditions.

WOS
Держатели документа:
[Bezrukikh, Anna
Esimbekova, Elena
Nemtseva, Elena
Kratasyuk, Valentina
Shimomura, Osamu] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia
[Esimbekova, Elena
Nemtseva, Elena
Kratasyuk, Valentina] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bezrukikh, A...; Esimbekova, E...; Nemtseva, E...; Kratasyuk, V...; Shimomura, O...; Government of Russian Federation [11.G34.31.0058]; Russian Academy of Sciences [6.8]; Ministry of Education and Science [1762]; Siberian Federal University [1762]

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