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Progress in the Study of Bioluminescent Earthworms / N. S. Rodionova [et al.] // Photochem. Photobiol. - 2017. - Vol. 93, Is. 2. - P416-428, DOI 10.1111/php.12709 . - ISSN 0031-8655
Аннотация: Even though bioluminescent oligochaetes rarely catch people's eyes due to their secretive lifestyle, glowing earthworms sighting reports have come from different areas on all continents except Antarctica. A major breakthrough in the research of earthworm bioluminescence occurred in the 1960s with the studies of the North American Diplocardia longa. Comparative studies conducted on 13 earthworm species belonging to six genera showed that N-isovaleryl-3-aminopropanal (Diplocardia luciferin) is the common substrate for bioluminescence in all examined species, while luciferases appeared to be responsible for the color of bioluminescence. The second momentous change in the situation has occurred with the discovery in Siberia (Russia) of two unknown luminous enchytraeids. The two bioluminescent systems belong to different types, have different spectral characteristics and localization, and different temperature and pH optima. They are unique, and this fact is confirmed by the negative results of all possible cross-reactions. The bioluminescent system of Henlea sp. comprises four essential components: luciferase, luciferin, oxygen and calcium ion. For Friderica heliota, the luminescent reaction requires five components: luciferase, luciferin, ATP, magnesium ion and oxygen. Along with luciferin, more than a dozen analogues were isolated from worm biomass. These novel peptide-like natural compounds represent an unprecedented chemistry found in terrestrial organisms. © 2017 The American Society of Photobiology

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Держатели документа:
Laboratory of Photobiology, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Department of Physics, Earth and Environmental Sciences, University of Siena, Siena, Italy
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Pirogov Russian National Research Medical University, Moscow, Russian Federation

Доп.точки доступа:
Rodionova, N. S.; Rota, E.; Tsarkova, A. S.; Petushkov, V. N.

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577.34
И 73


    Интенсивность накопления урана-238 представителями разных экологических уровней экосистемы р. Енисей [Текст] / Сибирское Отделение Российской академии наук, Институт биофизики СО РАН ; Институт биофизики СО РАН // Вестник Томского государственного университета. Биология. - 2016. - № 2 (34) . - С. 161-171DOI 10.17223/19988591/34/11
   Перевод заглавия: Accumulation of uranium-238 by representatives of different ecological levels in the Yenisei River ecosystem

УДК

577.34

597.5

Кл.слова (ненормированные):
коэффициент накопления -- трофический уровень -- Philolimnogammarus viridis -- Fointinalis antipyretica -- Thymallus arcticus -- Esox lucius)
Аннотация: В результате многолетних исследований (2010-2013) получены данные по накоплению урана-238 в гидробионтах разных трофических уровней экосистемы р. Енисей. Пробы водного мха (Fointinalis antipyretica), зообентоса (Philolimnogammarus viridis и Ph. Cyaneus), сибирского хариуса (Thymallus arcticus) и щуки (Esox lucius) отбирали в районе ранее зарегистрированного повышенного содержания урана в воде р. Енисей (вблизи радиоактивных сбросов Горно-химического комбината Росатома). Показано, что уран способен эффективно накапливаться из воды в биомассе всех исследованных гидробионтов. Максимальные коэффициенты накопления (КН) урана получены для водного мха, что многократно превышает КН урана для зообентоса и мышечной ткани рыб. Средние значения КН урана для зообентоса статистически значимо превышают КН в мышцах рыб за весь период наблюдения. Не выявлено статистически значимых отличий в накоплении урана рыбами разного трофического уровня (хариус и щука)
The aim of the research was a comparative evaluation of the intensity of uranium-238 accumulation in the biomass of aquatic organisms, occupying different trophic levels in the ecosystem of the Yenisei river. Previously, uranium-238 at concentrations higher than the background ones was detected in water and sediment samples of the Yenisei River collected close to the discharge site of one of the Rosatom facilities (the Mining-and-Chemical Combine). However, radioecological studies of the Yenisei River ecosystem have not focused on uranium isotopes in aquatic organisms so far. The toxic effects of uranium on living organisms are known to be based on its both radioactive and chemical properties. We conducted a study on accumulation of uranium-238 in aquatic organisms at different trophic levels in the Yenisei River ecosystem between 2010 and 2013. We collected samples of aquatic moss (Fointinalis antipyretica), zoobenthos (Philolimnogammarus viridis and Ph. cyaneus), arctic grayling (Thymallus arcticus), and pike (Esox lucius) from the Yenisei region at a distance of 85-90 km downstream of Krasnoyarsk (5-10 km downstream of the radioactive discharge of the Mining-and-Chemical Combine). Data on samples are shown in Table 1. We determined uranium concentration in the samples of aquatic organisms by neutron activation analysis, in water - by ICP-MS (Table 2). The study shows that uranium can be effectively concentrated from water and accumulate in the biomass of all aquatic organisms used in experiments. The highest uranium concentration factors (CFs) were obtained for aquatic moss, and they were several times higher than the uranium CFs for zoobenthos and fish muscles. Based on the uranium concentration from water, all aquatic organisms of the Yenisei River used in this study can be ranked as follows: aquatic moss > gammarids > grayling = pike. No statistically significant difference has been found between uranium accumulation by fish of different trophic levels (Arctic grayling and pike). The uranium CFs obtained in this study is comparable with the literature data on the CFs of artificial radionuclides for aquatic moss and fish of the Yenisei River

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Держатели документа:
Институт биофизики Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Болсуновский, Александр Яковлевич; Трофимова, Елена Александровна; Дементьев, Дмитрий Владимирович; Карпов, Антон Дмитриевич; Институт биофизики Сибирского отделения Российской Академии наук (Красноярск)

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Bacterial Communities of Three Saline Meromictic Lakes in Central Asia [Text] / B. Baatar [et al.] // PLoS One. - 2016. - Vol. 11, Is. 3. - Ст. e0150847, DOI 10.1371/journal.pone.0150847. - Cited References:65. - This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99- 2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a.; We thank our Russian and Mongolian colleagues in the Institute of Biophysics SB RAS and National University of Mongolia for assistance with providing samples and hydro-parameter data. This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99-2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
PURPLE SULFUR BACTERIA
REAL-TIME PCR
MICROBIAL COMMUNITIES
Аннотация: Meromictic lakes located in landlocked steppes of central Asia (similar to 2500 km inland) have unique geophysiochemical characteristics compared to other meromictic lakes. To characterize their bacteria and elucidate relationships between those bacteria and surrounding environments, water samples were collected from three saline meromictic lakes (Lakes Shira, Shunet and Oigon) in the border between Siberia and the West Mongolia, near the center of Asia. Based on in-depth tag pyrosequencing, bacterial communities were highly variable and dissimilar among lakes and between oxic and anoxic layers within individual lakes. Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria and Firmicutes were the most abundant phyla, whereas three genera of purple sulfur bacteria (a novel genus, Thiocapsa and Halochromatium) were predominant bacterial components in the anoxic layer of Lake Shira (similar to 20.6% of relative abundance), Lake Shunet (similar to 27.1%) and Lake Oigon (similar to 9.25%), respectively. However, few known green sulfur bacteria were detected. Notably, 3.94% of all sequencing reads were classified into 19 candidate divisions, which was especially high (23.12%) in the anoxic layer of Lake Shunet. Furthermore, several hydro-parameters (temperature, pH, dissolved oxygen, H2S and salinity) were associated (P0.05) with variations in dominant bacterial groups. In conclusion, based on highly variable bacterial composition in water layers or lakes, we inferred that the meromictic ecosystem was characterized by high diversity and heterogenous niches.

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Держатели документа:
Acad Sinica, Taiwan Int Grad Program, Mol & Biol Agr Sci Program, Taipei 115, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan.
Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan.
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia.
Natl Pingtung Univ Sci & Technol, Pingtung 91201, Taiwan.
Natl Univ Mongolia, Sch Art & Sci, Ulaanbaatar 14201, Mongol Peo Rep.
Natl Chung Hsing Univ, Ctr Biotechnol, Taichung 40227, Taiwan.

Доп.точки доступа:
Baatar, Bayanmunkh; Chiang, Pei-Wen; Rogozin, Denis Yu; Wu, Yu-Ting; Tseng, Ching-Hung; Yang, Cheng-Yu; Chiu, Hsiu-Hui; Oyuntsetseg, Bolormaa; Degermendzhy, Andrey G.; Tang, Sen-Lin; National Sciences Council of Taiwan [NSC99- 2923-B-001-001-MY3, NSC 102-2923-B-001-004, NSC101-2923-B-001-003-MY3, NSC99-2923-B-001-001-MY3]; Biodiversity Research Center, Academia Sinica, Taiwan; Russian Foundation for Basic Research [14-04-01060-a]

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Methanolobus psychrotolerans sp. nov., a psychrotolerant methanoarchaeon isolated from a saline meromictic lake in siberia / S. -C. Chen [et al.] // Int. J. Syst. Evol. Microbiol. - 2018. - Vol. 68, Is. 4. - P1378-1383, DOI 10.1099/ijsem.0.002685 . - ISSN 1466-5026
Кл.слова (ненормированные):
Archaea -- Lake shira -- Methanogen -- Methanolobus -- Psychrotolerant -- Saline meromictic lake
Аннотация: A psychrotolerant, methylotrophic methanogen, strain YSF-03T, was isolated from the saline meromictic Lake Shira in Siberia. Cells of strain YSF-03T were non-motile, irregular cocci and 0.8–1.2?m in diameter. The methanogenic substrates utilized by strain YSF-03T were methanol and trimethylamine. The temperature range of growth for strain YSF-03T was from 0 to 37 °C. The optimum growth conditions were 30–37 °C, pH 7.0–7.4 and 0.17M NaCl. The G+C content of the genome of strain YSF-03T was 41.3 mol%. Phylogenetic analysis revealed that strain YSF-03T was most closely related to Methanolobus profundi MobMT (98.15% similarity in 16S rRNA gene sequence). Genome relatedness between strain YSF-03T and MobMT was computed using the Genome-to-Genome Distance Calculator and average nucleotide identity, which gave values of 23.5 and 79.3 %, respectively. Based on the morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain YSF-03T represents a novel species of the genus Methanolobus, for which the name Methanolobus psychrotolerans sp. nov. is proposed. The type strain is YSF-03T (=BCRC AR10049T=DSM 104044T=NBRC 112514T). © 2018 IUMS.

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Держатели документа:
Department of Life Science, National Chung Hsing University, Taiwan
Agricultural Biotechnology Center, National Chung Hsing University, Taiwan
Biodiversity Research Center, Academia Sinica, Taiwan
Institute of Biophysics Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Chen, S. -C.; Huang, H. -H.; Lai, M. -C.; Weng, C. -Y.; Chiu, H. -H.; Tang, S. -L.; Rogozin, D. Y.; Degermendzhy, A. G.

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Low-temperature argon and ammonia plasma treatment of poly-3-hydroxybutyrate films: Surface topography and chemistry changes affect fibroblast cells in vitro / R. A. Surmenev [et al.] // Eur Polym J. - 2019. - Vol. 112. - P137-145, DOI 10.1016/j.eurpolymj.2018.12.040 . - ISSN 0014-3057
Кл.слова (ненормированные):
Cell adhesion -- Dielectric barrier discharge -- Plasma treatment -- Surface etching -- Wetting behaviour -- Ammonia -- Argon -- Cell adhesion -- Cell culture -- Chemical modification -- Contact angle -- Dielectric materials -- Electric discharges -- Fibroblasts -- Mammals -- Nitrogen plasma -- Plasma applications -- Surface roughness -- Surface treatment -- Temperature -- Topography -- Ammonia plasma treatment -- Dielectric barrier discharges -- Different proportions -- Plasma treatment -- Poly-3-hydroxybutyrate -- Poly3-hydroxybutyrate (PHB) -- Surface etching -- Wetting behaviour -- Wetting
Аннотация: Poly-3-hydroxybutyrate (PHB) films were plasma-treated using pure NH3, pure Ar or mixtures of the two different proportions (20%, 30%, 40%, 50%, 70% NH3 in Ar). Surface chemistry and surface topography changes of PHB films were observed after plasma processing in all plasma regimes. The XPS results confirmed the absence of chemical modification in the case of pure Ar plasma treatment. Nitrogen-containing groups (e.g., N–C[dbnd]O) were detected on the surfaces of P3HB films treated with NH3-containing plasma. The surfaces of the untreated P3HB films were hydrophobic, and plasma treatment turned the surfaces hydrophilic, irrespective of the treatment. A significant decrease in the contact angle and an increase in the free surface energy were observed. An insignificant surface ageing effect was observed when P3HB samples were exposed to air for 10 days. In NIH 3T3 mice fibroblast cells, cell adhesion was significantly improved after plasma treatment in an Ar atmosphere, which is likely related to the fact that there was a surface ? potential of 88.6 mV at neutral pH, causing a cleavage of the polymer chains and an increase in surface roughness. © 2018 Elsevier Ltd

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Держатели документа:
Physical Materials Science and Composite Materials Centre, National Research Tomsk Polytechnic University, Tomsk, 634050, Russian Federation
Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, 70569, Germany
Synchrotron Radiation Facility ANKA, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Surmenev, R. A.; Chernozem, R. V.; Syromotina, D. S.; Oehr, C.; Baumbach, T.; Krause, B.; Boyandin, A. N.; Dvoinina, L. M.; Volova, T. G.; Surmeneva, M. A.

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Production and properties of bacterial cellulose by the strain Komagataeibacter xylinus B-12068 / T. G. Volova [et al.] // Appl. Microbiol. Biotechnol. - 2018. - P1-12, DOI 10.1007/s00253-018-9198-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
Bacterial cellulose -- Growth conditions -- Komagataeibacter xylinus -- Biocompatibility -- Cell culture -- Cellulose -- Cultivation -- Glucose -- 3t3 mouse fibroblasts -- Bacterial cellulose -- Cultivation conditions -- Emission spectrometry -- Ethanol concentrations -- Growth conditions -- Komagataeibacter xylinus -- Physical and mechanical properties -- Substrates
Аннотация: A strain of acetic acid bacteria, Komagataeibacter xylinus B-12068, was studied as a source for bacterial cellulose (BC) production. The effects of cultivation conditions (carbon sources, temperature, and pH) on BC production and properties were studied in surface and submerged cultures. Glucose was found to be the best substrate for BC production among the sugars tested; ethanol concentration of 3% (w/v) enhanced the productivity of BC. Optimization of medium and cultivation conditions ensures a high production of BC on glucose and glycerol, up to 2.4 and 3.3 g/L/day, respectively. C/N elemental analysis, emission spectrometry, SEM, DTA, and X-ray were used to investigate the structure and physical and mechanical properties of the BC produced under different conditions. MTT assay and SEM showed that native cellulose membrane did not cause cytotoxicity upon direct contact with NIH 3T3 mouse fibroblast cells and was highly biocompatible. © 2018 Springer-Verlag GmbH Germany, part of Springer Nature

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Держатели документа:
Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Siberian Federal University, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Prudnikova, S. V.; Sukovatyi, A. G.; Shishatskaya, E. I.

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^a341.27.17.09.07.23.19^2VINITI
Г 51

Гительзон, И. И.
Регуляция биолюминесцентной системы бактерий и ее экологическая функция [Текст] : научное издание / И. И. Гительзон, Б. А. Илларионов // Всес. конф. Регуляция микроб. метаболизма, Пущино, 12-14 июня, 1989. - Пущино, 1989. - С. 144

ГРНТИ

34.27.17

РУБ 341.27.17.09.07.23.19
Рубрики:
СВЕТЯЩИЕСЯ БАКТЕРИИ
   БИОЛЮМИНЕСЦЕНЦИЯ
   БИОЛЮМИНЕСЦЕНТНАЯ СИСТЕМА
   ЭКОЛОГИЧЕСКАЯ ФУНКЦИЯ
   ЛЮЦИФЕРАЗА
   РЕГУЛЯЦИЯ
   РЕГУЛЯТОРНЫЕ ГЕНЫ
   LUX-ГЕНЫ
   КОНФЕРЕНЦИИ
   СССР
   ПУЩИНО
   1989
   ИЮНЬ
Аннотация: Способность фотобактерий излучать свет при соокислении восстановленного FMHH[2] и длинноцепочечного альдегида (C[1][0]-С[1][6]) обеспечена специфическим ферментом - люциферазой, представляющим собой гетеродимер с массой для 'ню'-субъединицы около 40 кДа и 'бета' - около 36 кД с незначительными межродовыми различиями. Гены, относящиеся к люминесцентной системе, тесно сцеплены, что позволило клонировать их в одном фрагменте ДНК. Lux-гены организованы в два оперона, расположенные в хромосоме рядом. Оперон L содержит ген Lux R, продукт которого участвует в регуляции биолюминесценции. Оперон R содержит пять генов: Lux I (регуляция биолюминесценции), Lux A и Lux B ('альфа'- и 'бета'-субъединицы люциферазы). У Ph. leiognathi нами обнаружен после Lux B ген Lux G, функция которого пока не выяснена, кодируемый им белок имеет 26% гомологию с 'бета'-субъединицей люциферазы. У светящихся бактерий обнаружена своеобразная, практически нигде более не встреченная система регуляции синтеза люциферазы через аутоиндукцию видоспецифическим метаболитом. Для N. fischeri это N-('бета'-кетакапроил) гомосеринлактон, его синтез определяется Lux I геном. В Кл аутоиндуктор связывается с рецепторным белком, продуктом Lux R-гена. При достижении пороговой конц-ии этот комплекс активирует транскрипцию R-оперона. Аутоиндуктор свободно проникает через клеточную мембрану. Т. обр., синтез люциферазы регулируется плотностью культуры бактерий. Показано, что светящиеся бактерии в море встречаются преимущественно в форме агрегаций, то есть в культурах высокой плотности; в этих условиях их свечение, стимулированное аутоиндуктором, привлекает потенциального хозяина - рыб, моллюсков, в кишечнике к-рых они наиболее часто встречаются в роли факультативных симбионтов. Свечение бактерий, благодаря легкости его регистрации, представляет собой исключительно благородный объект для изучения регуляции бактериального метаболизма.

Доп.точки доступа:
Илларионов, Б.А.

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Comparative study of temperature effects on bacterial luciferases [Text] / N. A. Tyulkova, T. P. Sandalova // Biochem.-Moscow. - 1996. - Vol. 61, Is. 2. - P. 205-214. - Cited References: 23 . - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
BIOLUMINESCENCE
Кл.слова (ненормированные):
bacterial luciferase -- temperature -- activation energy
Аннотация: Effects of temperature on bioluminescent patterns of luciferases from luminescent bacteria Vibrio harveyi, Vibrio fischeri, Photobacterium leiognathi, and Photobacterium phosphoreum were studied. The highest luminescence level was observed at 15-25 degrees C for the luciferase from P. phosphoreum, at 20-30 degrees C for the V. fischeri and P. leiognathi enzymes, and at 30-37 degrees C for the enzyme from V. harveyi. All the luciferases were significantly stabilized at increased salt concentrations, at low pH values, or in the presence of dithiothreitol (DTT) and EDTA. The addition of DTT and EDTA affected the reversible stage of enzyme inactivation, while salts reduced the rate of the irreversible stage. A peak corresponding to aggregated protein was detected by gel chromatography of irreversibly inactivated luciferase. Activation energies were calculated for each luciferase in bioluminescent reactions with decanal, dodecanal, tetradecanal, and without aldehydes. The activation energy of the reaction with tetradecanal was much lower than those with the other aldehydes. The temperature dependence of the lifetime of the long-lived reaction intermediate showed that in the 10-30 degrees C interval all the luciferases, except for the enzyme from V. harveyi, have only one active form.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tyulkova, N.A.; Sandalova, T.P.

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INFLUENCE OF THE PH OF THE MEDIUM ON THE GROWTH AND PHYSIOLOGY OF HYDROGEN OXIDIZING BACTERIA [Text] / T. G. VOLOVA [et al.] // Microbiology. - 1986. - Vol. 55, Is. 5. - P. 585-589. - Cited References: 20 . - ISSN 0026-2617

РУБ Microbiology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
VOLOVA, T.G.; TERESHKOVA, G.M.; KALACHEVA, G.S.; SALNIKOV, M.V.

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10.

Gelatin and starch as stabilizers of the coupled enzyme system of luminous bacteria NADH:FMN-oxidoreductase-luciferase [Text] / A. . Bezrukikh [et al.] // Anal. Bioanal. Chem. - 2014. - Vol. 406, Is. 23. - P5743-5747, DOI 10.1007/s00216-014-7987-1. - Cited References: 14. - The work was supported by the Program of the Government of Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant no. 11.G34.31.0058), the Russian Academy of Sciences (program "Molecular and Cell Biology", grant no. 6.8), and the state contract between the Ministry of Education and Science and Siberian Federal University, no. 1762. . - ISSN 1618-2642. - ISSN 1618-2650

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
IMMOBILIZATION
CHEMISTRY
Кл.слова (ненормированные):
Bacterial luciferase -- NADH:FMN-oxidoreductase -- Bioluminescence -- Stabilization of enzymes -- Gelatin -- Starch
Аннотация: We have studied the effects of a gel-like environment on the characteristics of enzyme preparations based on the coupled enzyme system of luminous bacteria, NADH:FMN-oxidoreductase-luciferase, to design a stable immobilizing reagent for bioluminescent analysis. Natural polymers, gelatin and starch, were used to create a viscous, structured microenvironment. The stability of the coupled enzyme system to such physical and chemical environmental factors as temperature, pH, and ionic strength in gelatin and starch-containing media was examined. It was shown that both gelatin and starch have a stabilizing effect on the enzymes of luminous bacteria under specific conditions. In particular, the enzymes' activity is increased twofold in the presence of 1 and 5 % of gelatin at 20 A degrees C and 25 A degrees C, respectively (temperatures lower than the gel point). Also, the acceptable pH range of the coupled enzyme system expands into the alkaline region and becomes 6.8-8.1. Stabilization at low ionic strength (0.01-0.06 mol L-1) is observed. At the same time, microenvironments based on either gelatin or starch do not change the enzymes' thermal inactivation rate constants in the temperature range from 25 to 43 A degrees C. Finally, gelatin and starch are suitable for development of a reagent for immobilization of enzymes which would be stable and resistant to physical and chemical environmental conditions.

WOS
Держатели документа:
[Bezrukikh, Anna
Esimbekova, Elena
Nemtseva, Elena
Kratasyuk, Valentina
Shimomura, Osamu] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia
[Esimbekova, Elena
Nemtseva, Elena
Kratasyuk, Valentina] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bezrukikh, A...; Esimbekova, E...; Nemtseva, E...; Kratasyuk, V...; Shimomura, O...; Government of Russian Federation [11.G34.31.0058]; Russian Academy of Sciences [6.8]; Ministry of Education and Science [1762]; Siberian Federal University [1762]

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11.

Practical enzymology course based on bioluminescence [Text] / V. A. Kratasyuk, I. Y. Kudinova // Luminescence. - 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P. 189-192, DOI 10.1002/(SICI)1522-7243(199907/08)14:4189::AID-BIO5273.0.CO;2-E. - Cited References: 7 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Кл.слова (ненормированные):
enzyme -- science education -- luciferase -- bioluminescence
Аннотация: We describe our experience with laboratory courses in enzymology based on the phenomenon of bioluminescence. The soluble and immobilized enzymes of luminous bacteria are used and the practical enzymological course consists of four main courses: (1) training in measuring the activities of soluble and immobilized enzymes; (2) the investigation of kinetic characteristics (kinetic constants) and enzyme-substrate and enzyme-inhibitor interactions in the bacterial bioluminescent reaction; (3) The testing of physico-chemical characteristics of enzymes (pH, temperature, ion strength, etc.); (4) the effect of inhibitors on enzymes. Training is possible in groups of about ten persons. Our practice work has been introduced in the biological, pedagogical and physical departments of Krasnoyarsk State University. Students of the pedagogical department have created a popular and interesting series of laboratory works for high school children aged 14-17 years. Copyright (C) 1999 John Wiley & Sons, Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Kudinova, I.Y.

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12.

Designing a reusable system based on nanodiamonds for biochemical determination of urea / N. O. Ronzhin [et al.] // Doklad. Biochem. Biophys. - 2015. - Vol. 465, Is. 1. - P428-431, DOI 10.1134/S1607672915060216 . - ISSN 1607-6729
Аннотация: A reusable system including urease covalently bound to the surface of modified nanodiamonds (MNDs) has been developed for the multiple determination of urea. The immobilized enzyme exhibits functional activity and catalyzes the hydrolysis of urea to yield ammonia. The presence of ammonia is confirmed by the formation of a colored product after the addition of chemical reagents. It was shown that the MNDs-urease complex can function in a wide range of temperatures and pH as well as in deionized water. The complex provides a linear yield of the product at low analyte concentrations and allows the multiple determination of urea in vitro. © 2015, Pleiades Publishing, Ltd.

Scopus,
WOS
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ronzhin, N. O.; Baron, A. V.; Bondar, V. S.; Gitelson, I. I.

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13.

Oxygen and ammonia plasma treatment of poly(3-hydroxybutyrate) films for controlled surface zeta potential and improved cell compatibility [Text] / D. S. Syromotina [et al.] // Mater. Lett. - 2016. - Vol. 163. - P277-280, DOI 10.1016/j.matlet.2015.10.080. - Cited References:10. - The authors thank Mr. T.M Mukhametkaliyev, Mrs. A.A. Sharonova and Dr. A. Wittmar for their assistance with the experiments. This research was supported by the Russian President's Stipend SP-6664.2013.4, Ministry for Education and Science Grant MK-485.2014.8, the State order NAUKA #11.1359.2014/K, and the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 0167-577X. - ISSN 1873-4979

РУБ Materials Science, Multidisciplinary + Physics, Applied

Кл.слова (ненормированные):
Plasma treatment -- Polymer -- Cell adhesion -- Surface modification
Аннотация: The oxygen and ammonia radio-frequency (RF) plasma treatment of poly(3-hydroxybutyrate) P3HB films was performed. We revealed significant changes in the topography, a decrease in the surface zeta potential from -63 to -75 mV after the oxygen-plasma treatment and an increase after ammonia plasma treatment from -63 to -45 mV at a pH of 7.4. Investigations into the NIH 3T3 fibroblast adhesion and growth demonstrated the best cell vitality and a higher cell number for the ammonia plasma treatment at 150W. (C) 2015 Elsevier B.V. All rights reserved.

WOS
Держатели документа:
Natl Res Tomsk Polytech Univ, Tomsk 634050, Russia.
Fraunhofer Inst Interfacial Engn & Biotechnol IGB, D-70569 Stuttgart, Germany.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Univ Duisburg Essen, Inorgan Chem, D-45117 Essen, Germany.
Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45117 Essen, Germany.
Univ Duisburg Essen, Tech Chem 2, D-45141 Essen, Germany.
Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45141 Essen, Germany.

Доп.точки доступа:
Syromotina, D. S.; Surmenev, R. A.; Surmeneva, M. A.; Boyandin, A. N.; Epple, M.; Ulbricht, M.; Oehr, C.; Volova, T. G.; Russian President's Stipend [SP-6664.2013.4]; Ministry for Education and Science Grant [MK-485.2014.8]; State order NAUKA [11.1359.2014/K]; State budget [01201351505]

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14.

High-resolution structures of scytalone dehydratase-inhibitor complexes crystallized at physiological pH [Text] / Z. . Wawrzak [et al.] // Proteins. - 1999. - Vol. 35, Is. 4. - P. 425-439, DOI 10.1002/(SICI)1097-0134(19990601)35:4425::AID-PROT63.0.CO;2-1. - Cited References: 33 . - ISSN 0887-3585

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
MAGNAPORTHE-GRISEA
   HEMAGGLUTININ
   GLYCOPROTEIN
   REFINEMENT
   MELANIN
   DISEASE
   SITE
Кл.слова (ненормированные):
structure-based design -- enzyme inhibitors -- X-ray crystallography -- fungicides -- melanin biosynthesis
Аннотация: Scytalone dehydratase is a molecular target of inhibitor design efforts aimed at preventing the fungal disease caused by Magnaporthe grisea. A method for cocrystallization of enzyme with inhibitors at neutral pH has produced several crystal structures of enzyme-inhibitor complexes at resolutions ranging from 1.5 to 2.2 Angstrom Four high resolution structures of different enzyme-inhibitor complexes are described. In contrast to the original X-ray structure of the enzyme, the four new structures have well-defined electron density for the loop region comprising residues 115-119 and a different conformation between residues 154 and 160. The structure of the enzyme complex with an aminoquinazoline inhibitor showed that the inhibitor is in a position to form a hydrogen bond with the amide of the Asn131 side chain and with two water molecules in a fashion similar to the salicylamide inhibitor in the original structure, thus confirming design principles. The aminoquinazoline structure also allows for a more confident assignment of donors and accepters in the hydrogen bonding network, The structures of the enzyme complexes with two dichlorocyclopropane carboxamide inhibitors showed the two chlorine atoms nearly in plane with the amide side chain of Asn131. The positions of Phe53 and Phe158 are significantly altered in the new structures in comparison to the two structures obtained from crystals grown at acidic pH, The multiple structures help define the mobility of active site amino acids critical for catalysis and inhibitor binding. Proteins 1999;35:425-439. (C) 1999 Wiley-Liss, Inc.

WOS
Держатели документа:
Dupont Co, Stine Haskell Res Ctr, Agr Prod, Newark, DE 19714 USA
Dupont Co, Expt Stn, Life Sci, Wilmington, DE USA
Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Wawrzak, Z...; Sandalova, T...; Steffens, J.J.; Basarab, G.S.; Lundqvist, T...; Lindqvist, Y...; Jordan, D.B.

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15.

Experimental approach to study the effect of mutations on the protein folding pathway / E. V. Nemtseva [et al.] // PLoS One. - 2019. - Vol. 14, Is. 1. - Ст. e0210361, DOI 10.1371/journal.pone.0210361. - Cited References:38. - The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Projects 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Project 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
FLUORESCENCE LIFETIMES ORIGIN
   TRANSITION-STATE
   EXCHANGE
   TRYPTOPHAN
Аннотация: Is it possible to compare the physicochemical properties of a wild-type protein and its mutant form under the same conditions? Provided the mutation has destabilized the protein, it may be more correct to compare the mutant protein under native conditions to the wild-type protein destabilized with a small amount of the denaturant. In general, is it appropriate to compare the properties of proteins destabilized by different treatments: mutations, pH, temperature, and denaturants like urea? These issues have compelled us to search for methods and ways of presentation of experimental results that would allow a comparison of mutant forms of proteins under different conditions and lead to conclusions on the effect of mutations on the protein folding/unfolding pathway. We have studied equilibrium unfolding of wild-type bovine carbonic anhydrase II (BCA II) and its six mutant forms using different urea concentrations. BCA II has been already studied in detail and is a good model object for validating new techniques. In this case, time-resolved fluorescence spectroscopy was chosen as the basic research method. The main features of this experimental method allowed us to compare different stages of unfolding of studied proteins and prove experimentally that a single substitution of the amino acid in three mutant forms of BCA II affected the native state of the protein but did not change its unfolding pathway. On the contrary, the inserted disulfide bridge in three other mutant forms of BCA II affected the protein unfolding pathway. An important result of this research is that we have validated the new approach allowing investigation of the effect of mutations on the folding of globular proteins, because in this way it is possible to compare proteins in the same structural states rather than under identical conditions.

WOS,
Смотреть статью,
Scopus
Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Prot Res, Pushchino, Moscow Region, Russia.

Доп.точки доступа:
Nemtseva, Elena V.; Gerasimova, Marina A.; Melnik, Tatiana N.; Melnik, Bogdan S.; Gerasimova, Marina; Nemtseva, Elena; Ministry of Science and Education of the Russian Federation [6.7734.2017]; Russian Science Foundation [N14-24-00157]

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16.

Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications / O. Mogilnaya, N. Ronzhin, E. Posokhina, V. Bondar // Protein J. - 2021, DOI 10.1007/s10930-021-10010-z. - Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, Project No. 0356-2019-0022. . - Article in press. - ISSN 1572-3887. - ISSN 1573-4943

РУБ Biochemistry & Molecular Biology
Рубрики:
ARYL-ALCOHOL OXIDASE
   GLUCOSE-OXIDASE
   PEROXIDASES
   MECHANISM
Кл.слова (ненормированные):
Extracellular oxidase -- Flavoprotein -- Fungi -- Phenol
Аннотация: The extracellular enzyme with oxidase function was extracted from the Neonothopanus nambi luminescent fungus by using mild processing of mycelium with beta-glucosidase and then isolated by gel-filtration chromatography. The extracted enzyme is found to be a FAD-containing protein, catalyzing phenol co-oxidation with 4-aminoantipyrine without addition of H2O2, which distinguishes it from peroxidases. This fact allowed us to assume that this enzyme may be a mixed-function oxidase. According to gel-filtration chromatography and SDS-PAGE, the oxidase has molecular weight of 60 kDa. The enzyme exhibits maximum activity at 55-70 degrees C and pH 5.0. Kinetic parameters K-m and V-max of the oxidase for phenol were 0.21 mM and 0.40 mu M min(-1). We suggest that the extracted enzyme can be useful to develop a simplified biosensor for colorimetric detection of phenol in aqueous media, which does not require using hydrogen peroxide.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Mogilnaya, Olga; Ronzhin, Nikita; Posokhina, Ekaterina; Bondar, Vladimir; [0356-2019-0022]

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17.

Spatial and temporal variation in Arctic freshwater chemistry—Reflecting climate-induced landscape alterations and a changing template for biodiversity / B. J. Huser, M. N. Futter, D. Bogan [et al.] // Freshw. Biol. - 2020, DOI 10.1111/fwb.13645 . - Article in press. - ISSN 0046-5070
Кл.слова (ненормированные):
biogeochemistry -- eutrophication -- lakes -- oligotrophication -- rivers
Аннотация: Freshwater chemistry across the circumpolar region was characterised using a pan-Arctic data set from 1,032 lake and 482 river stations. Temporal trends were estimated for Early (1970–1985), Middle (1986–2000), and Late (2001–2015) periods. Spatial patterns were assessed using data collected since 2001. Alkalinity, pH, conductivity, sulfate, chloride, sodium, calcium, and magnesium (major ions) were generally higher in the northern-most Arctic regions than in the Near Arctic (southern-most) region. In particular, spatial patterns in pH, alkalinity, calcium, and magnesium appeared to reflect underlying geology, with more alkaline waters in the High Arctic and Sub Arctic, where sedimentary bedrock dominated. Carbon and nutrients displayed latitudinal trends, with lower levels of dissolved organic carbon (DOC), total nitrogen, and (to a lesser extent) total phosphorus (TP) in the High and Low Arctic than at lower latitudes. Significantly higher nutrient levels were observed in systems impacted by permafrost thaw slumps. Bulk temporal trends indicated that TP was higher during the Late period in the High Arctic, whereas it was lower in the Near Arctic. In contrast, DOC and total nitrogen were both lower during the Late period in the High Arctic sites. Major ion concentrations were higher in the Near, Sub, and Low Arctic during the Late period, but the opposite bulk trend was found in the High Arctic. Significant pan-Arctic temporal trends were detected for all variables, with the most prevalent being negative TP trends in the Near and Sub Arctic, and positive trends in the High and Low Arctic (mean trends ranged from +0.57%/year in the High/Low Arctic to ?2.2%/year in the Near Arctic), indicating widespread nutrient enrichment at higher latitudes and oligotrophication at lower latitudes. The divergent P trends across regions may be explained by changes in deposition and climate, causing decreased catchment transport of P in the south (e.g. increased soil binding and trapping in terrestrial vegetation) and increased P availability in the north (deepening of the active layer of the permafrost and soil/sediment sloughing). Other changes in concentrations of major ions and DOC were consistent with projected effects of ongoing climate change. Given the ongoing warming across the Arctic, these region-specific changes are likely to have even greater effects on Arctic water quality, biota, ecosystem function and services, and human well-being in the future. © 2020 The Authors. Freshwater Biology published by John Wiley & Sons Ltd.

Scopus
Держатели документа:
Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Uppsala, Sweden
Alaska Center for Conservation Science, University of Alaska Anchorage, Anchorage, AK, United States
Norwegian Water Resources & Energy Directorate, Oslo, Norway
Natural History Museum, University of Oslo, Oslo, Norway
Cold Regions Research Centre, Wilfrid Laurier University, Waterloo, ON, Canada
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Department of Ecology and Environmental Science, Climate Impacts Research Centre, Umea University, Abisko, Sweden
Paleoecological Environmental Assessment and Research Laboratory (PEARL), Department of Biology, Queen’s University, Kingston, ON, Canada
Norwegian Institute for Nature Research, Oslo, Norway
Canadian Rivers Institute and Department of Biology, University of New Brunswick, Fredericton, NB, Canada

Доп.точки доступа:
Huser, B. J.; Futter, M. N.; Bogan, D.; Brittain, J. E.; Culp, J. M.; Goedkoop, W.; Gribovskaya, I.; Karlsson, J.; Lau, D. C.P.; Ruhland, K. M.; Schartau, A. K.; Shaftel, R.; Smol, J. P.; Vrede, T.; Lento, J.

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18.

Spatial and temporal variation in Arctic freshwater chemistry-Reflecting climate-induced landscape alterations and a changing template for biodiversity / B. J. Huser, M. N. Futter, D. Bogan [et al.] // Freshw. Biol. - 2020, DOI 10.1111/fwb.13645. - Cited References:98. - Environment and Climate Change Canada; Cumulative Impact Monitoring Program, Government of Northwest Territories . - Article in press. - ISSN 0046-5070. - ISSN 1365-2427

РУБ Ecology + Marine & Freshwater Biology
Рубрики:
DISSOLVED ORGANIC-CARBON
PERMAFROST THAW
CHEMICAL LIMNOLOGY
Кл.слова (ненормированные):
biogeochemistry -- eutrophication -- lakes -- oligotrophication -- rivers
Аннотация: Freshwater chemistry across the circumpolar region was characterised using a pan-Arctic data set from 1,032 lake and 482 river stations. Temporal trends were estimated for Early (1970-1985), Middle (1986-2000), and Late (2001-2015) periods. Spatial patterns were assessed using data collected since 2001. Alkalinity, pH, conductivity, sulfate, chloride, sodium, calcium, and magnesium (major ions) were generally higher in the northern-most Arctic regions than in the Near Arctic (southern-most) region. In particular, spatial patterns in pH, alkalinity, calcium, and magnesium appeared to reflect underlying geology, with more alkaline waters in the High Arctic and Sub Arctic, where sedimentary bedrock dominated. Carbon and nutrients displayed latitudinal trends, with lower levels of dissolved organic carbon (DOC), total nitrogen, and (to a lesser extent) total phosphorus (TP) in the High and Low Arctic than at lower latitudes. Significantly higher nutrient levels were observed in systems impacted by permafrost thaw slumps. Bulk temporal trends indicated that TP was higher during the Late period in the High Arctic, whereas it was lower in the Near Arctic. In contrast, DOC and total nitrogen were both lower during the Late period in the High Arctic sites. Major ion concentrations were higher in the Near, Sub, and Low Arctic during the Late period, but the opposite bulk trend was found in the High Arctic. Significant pan-Arctic temporal trends were detected for all variables, with the most prevalent being negative TP trends in the Near and Sub Arctic, and positive trends in the High and Low Arctic (mean trends ranged from +0.57%/year in the High/Low Arctic to -2.2%/year in the Near Arctic), indicating widespread nutrient enrichment at higher latitudes and oligotrophication at lower latitudes. The divergent P trends across regions may be explained by changes in deposition and climate, causing decreased catchment transport of P in the south (e.g. increased soil binding and trapping in terrestrial vegetation) and increased P availability in the north (deepening of the active layer of the permafrost and soil/sediment sloughing). Other changes in concentrations of major ions and DOC were consistent with projected effects of ongoing climate change. Given the ongoing warming across the Arctic, these region-specific changes are likely to have even greater effects on Arctic water quality, biota, ecosystem function and services, and human well-being in the future.

WOS
Держатели документа:
Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, Box 7050, S-75007 Uppsala, Sweden.
Univ Alaska Anchorage, Alaska Ctr Conservat Sci, Anchorage, AK USA.
Norwegian Water Resources & Energy Directorate, Oslo, Norway.
Univ Oslo, Nat Hist Museum, Oslo, Norway.
Wilfrid Laurier Univ, Cold Regions Res Ctr, Waterloo, ON, Canada.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Umea Univ, Climate Impacts Res Ctr, Dept Ecol & Environm Sci, Umea, Sweden.
Queens Univ, Dept Biol, Paleoecol Environm Assessment & Res Lab PEARL, Kingston, ON, Canada.
Norwegian Inst Nat Res, Oslo, Norway.
Univ New Brunswick, Canadian Rivers Inst, Fredericton, NB, Canada.
Univ New Brunswick, Dept Biol, Fredericton, NB, Canada.

Доп.точки доступа:
Huser, Brian J.; Futter, Martyn N.; Bogan, Daniel; Brittain, John E.; Culp, Joseph M.; Goedkoop, Willem; Gribovskaya, Iliada; Karlsson, Jan; Lau, Danny C. P.; Ruhland, Kathleen M.; Schartau, Ann Kristin; Shaftel, Rebecca; Smol, John P.; Vrede, Tobias; Lento, Jennifer; Environment and Climate Change Canada; Cumulative Impact Monitoring Program, Government of Northwest Territories

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19.

Positive feedback between acidosis and hypoxia during the transition of COVID-19 to a severe form of the disease / D. Lagutkin, D. Semyonov, Y. E. Yegorov [et al.] // FEBS Open Bio. - 2021. - Vol. 11. - P450-450. - Cited References:0 . - ISSN 2211-5463

РУБ Biochemistry & Molecular Biology

Аннотация: SARSCoV2 virus causes disease that has several distinctive characteristics compared to diseases caused by other viruses. We have put forward a hypothesis that relates COVID19 pathogenesis with acidosis, which frequently characterizes severe cases of this disease. It has been shown that hypoxia and acidosis affect the progression of severe COVID19 at various physiological levels such as organs, tissues, and cells. The physiological effects of acidosis and hypoxia range from the level of compensatory capabilities of the whole organism to the functioning of a single hemoglobin molecule. In our work, we consider several mechanisms that link the damaging factors of COVID19 with acidosis. These mechanisms reveal stepbystep processes with a pronounced positive feedback. In accordance with the well known Bohr effect, a decrease in blood pH leads to a drop in blood oxygen saturation. At the same time, this drop in saturation contributes to the further development of acidosis. This indicates a depletion of the body's compensatory capabilities to regulate acidosis and leads to deterioration of the patient's condition. In addition, a decrease in pH can cause conformational changes in the viral Sprotein, followed by changes in ability of some antibodies to recognize the virus. This might lead to the decrease in antibodies affinity and avidity, negatively affecting virus clearance. Low levels of pH and hypoxia in blood and tissues can induce a proinflammatory innate response even in the absence of antigen stimulation. Therefore, hypoxia and acidosis can lead to a restructuring of the immune system and multidirectional pro and antiinflammatory responses, which often, instead of recovery, lead to the disease aggravation.

WOS
Держатели документа:
Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow, Russia.
Voyno Yasenetsky Krasnoyarsk State Med Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Voronezh State Univ, Voronezh, Russia.
Lomonosov Moscow State Univ, Fac Phys, 1-2 Leninskie Gory, Moscow 119991, Russia.
Russian Acad Sci, Inst Analyt Instrumentat, Moscow, Russia.
Sendai Viralyt LLC, Acton, MA USA.

Доп.точки доступа:
Lagutkin, D.; Semyonov, D.; Yegorov, Y. E.; Lavrinenko, I.; Generalov, E.; Zaitceva, A. Y.; Matveeva, O.; Nechipurenko, Y. D.

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20.

^a341.47.51.15.19.13^2VINITI
A99

Aдсорбция афлатоксина В1 наноалмазами детонационного синтеза [Текст] : научное издание / А. П. Пузырь, К. В. Пуртов, О. А. Шендерова [и др.] // Докл. РАН. - 2007. - Т. 417, N 1. - С. 117-120 . - ISSN 0869-5652

ГРНТИ

34.47.51

РУБ 341.47.51.15.19.13
Рубрики:
ПИЩЕВЫЕ ПРОДУКТЫ
   КОРМА
   ЗАГРЯЗНЕНИЕ МИКОТОКСИНАМИ
   АФЛАТОКСИНЫ
   УДАЛЕНИЕ
   АДСОРБЕНТЫ
   ЭНТЕРОСОРБЕНТЫ
   НАНОАЛМАЗЫ
   ДЕТОНАЦИОННОГО СИНТЕЗА
Аннотация: Загрязнение кормов и продуктов питания токсинами микроскопических грибов, в частности афлатоксинами, представляет опасность для здоровья животных и человека. В качестве адсорбента для нейтрализации микотоксинов представляют интерес наноалмазы (НА) детонационного синтеза. Высокоразвитая поверхность НА, наличие на поверхности наночастиц различных химических активных функциональных групп, углеводородных фрагментов и микропримесей металлов определяют их высокие сорбционные свойства к биомолекулам. Установлена способность детонационных МНА адсорбировать АфB1 из водных растворов при кислых, слабокислых и щелочных значениях pH, причем время адсорбции микотоксина наночастицами не превышает 2-3 мин. Показано, что сорбционная емкость МНА к АфB1 зависит от соотношения количеств микотоксина и сорбента. В целом, полученные данные позволяют прогнозировать возможность практического применения данного наноматериала для энтеросорбции и нейтрализации микотоксинов, что является предметом дальнейших исследований. Россия, Ин-т биофизики СО РАН, Красноярск. Библ. 15

Доп.точки доступа:
Пузырь, А.П.; Пуртов, К.В.; Шендерова, О.А.; Луо, М.; Бреннер, Д.В.; Бондарь, В.С.

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