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Detonation Nanodiamonds as a New Tool for Phenol Detection in Aqueous Medium / N. Ronzhin, A. Puzyr, V. Bondar // J. Nanosci. Nanotechnol. - 2018. - Vol. 18, Is. 8. - P5448-5453, DOI 10.1166/jnn.2018.15382. - Cited References:27. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (Project No. 0356-2016-0709) and Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund to the research (Project No. 16-43-243027). . - ISSN 1533-4880. - ISSN 1533-4899

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
ENVIRONMENTAL-POLLUTANTS
   DRUG-DELIVERY
   PARTICLES
   CARBON
Кл.слова (ненормированные):
Detonation Nanodiamonds -- Catalytic Activity -- Metal Ions -- Azo Coupling -- Reaction -- Phenol Detection
Аннотация: This paper demonstrates the effectiveness of using detonation nanodiamonds (DNDs) for detecting phenol in aqueous medium. The study has shown that the catalytic effect of DNDs in the oxidative azo coupling reaction (phenol-4-aminoantipyrine-hydrogen peroxide) is produced by trace amounts of iron and copper ions adsorbed on the surface of nanoparticles. The effectiveness of DNDs as a catalyst is determined by the amounts of these adsorbates and can be enhanced by a factor of two by additional adsorption of these ions onto the nanoparticles. A rise in the temperature of the ONO-catalyzed azo coupling reaction leads to a considerable (4.5-fold) increase in the reaction product yield. DNDs used to detect phenol in aqueous medium enable a linear increase in the yield of the product of the azo coupling reaction at concentrations of the analyte of between 0.05 and 10 mu g/mlThe study demonstrates that DNDs can be reused to detect phenol in water samples.

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Держатели документа:
RAS, SB, Krasnoyarsk Sci Ctr, Inst Biophys,Fed Res Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ronzhin, Nikita; Puzyr, Alexey; Bondar, Vladimir; Russian Academy of Sciences [0356-2016-0709]; Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology [16-43-243027]

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Creation of Bifunctional Indicating Complex Based on Nanodiamonds and Extracellular Oxidases of Luminous Fungus Neonothopanus nambi / O. A. Mogilnaya [et al.] // Dokl. Biochem. Biophys. - 2018. - Vol. 480, Is. 1. - P135-138, DOI 10.1134/S160767291803002X. - Cited References:13 . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
PHANEROCHAETE-CHRYSOSPORIUM
NANOPARTICLES
Аннотация: A bifunctional indicating complex was created by immobilization of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MNDs) synthesized by detonation. It was found that the enzymes firmly adsorb onto MND particles and exhibit their catalytic activity. Model in vitro experiments showed that the created MND-enzymes complex is suitable for repeated use for analyte (glucose and phenol) testing and retains its activity after storage at 4 degrees C in deionized water for 1 month. The data obtained offer the prospects for developing a new class of reusable multifunctional indicating and diagnostic test systems on the basis of MNDs and higher fungal enzymes for medical and ecological analytics.

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Scopus
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk, Russia.

Доп.точки доступа:
Mogilnaya, O. A.; Ronzhin, N. O.; Artemenko, K. S.; Bondar, V. S.

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Nanodiamonds as an effective adsorbent for immobilization of extracellular peroxidases from luminous fungus Neonothopanus nambi to construct a phenol detection system / O. Mogilnaya [et al.] // Biocatal. Biotransform. - 2019. - Vol. 37, Is. 2. - P97-105, DOI 10.1080/10242422.2018.1472586. - Cited References:50. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences [project no. 0356-2016-0709]. . - ISSN 1024-2422. - ISSN 1029-2446

РУБ Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
CARBON NANOTUBES
   ARMILLARIA-BOREALIS
   LIGHT-EMISSION
   DEGRADATION
Кл.слова (ненормированные):
Nanodiamonds -- immobilization -- luminous fungus -- beta-glucosidase -- peroxidase -- indicator system
Аннотация: Modified nanodiamonds (MNDs) produced by detonation synthesis can be used as an effective adsorbent to immobilize extracellular peroxidases of the luminous basidiomycete Neonothopanus nambi. The enzymes are firmly immobilized on MND particles and exhibit catalytic activity. The indicator system (the MND-enzyme complex) reused many times retains its ability to catalyze reaction of co-oxidation of phenol and 4-aminoantipirine in the presence of hydrogen peroxide and remains functionally active during long-term storage (for 1 month or longer) in aqueous suspensions at 4 degrees C. MNDs and enzymes of higher fungi can be effectively used to construct new reusable indicator systems for analytical applications such as monitoring contamination of aquatic environments by phenolic compounds.

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Держатели документа:
RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr,SB, Krasnoyarsk, Russia.

Доп.точки доступа:
Mogilnaya, Olga; Ronzhin, Nikita; Artemenko, Karina; Bondar, Vladimir; Russian Academy of Sciences [0356-2016-0709]

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PHENOL BIODEGRADATION IN THE YENISEI RIVER AND THE KRASNOYARSK RESERVOIR, RUSSIA [Text] / M. I. GLADYSHEV, I. V. GRIBOVSKAYA ; ed.: NE Peters, RJ Allan, Allan, RJ // HYDROLOGICAL, CHEMICAL AND BIOLOGICAL PROCESSES OF TRANSFORMATION AND TRANSPORT OF CONTAMINANTS IN AQUATIC ENVIRONMENTS. Ser. IAHS PUBLICATIONS : INT ASSOC HYDROLOGICAL SCIENCES, 1994. - Hydrochemistry 1993 Symposium on Hydrological, Chemical and Biological Processes of Transformation and Transport of Contaminants in Aquatic Environments (MAY 24-28, 1993, ROSTOV NA DONU, RUSSIA), Is. 219. - P. 217-221. - Cited References: 0 . - ISBN 0144-7815. - ISBN 0-947571-88-4

РУБ Environmental Sciences + Toxicology + Water Resources

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Держатели документа:
RUSSIAN ACAD SCI,INST BIOPHYS,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
GLADYSHEV, M.I.; GRIBOVSKAYA, I.V.; Peters, NE \ed.\; Allan, RJ \ed.\

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Flocculation of bacterial cells in a culture with protozoans [Текст] / Y. L. Gurevich, M. I. Teremova, A. V. Latyntseva // Izv. Akad. Nauk Ser. Biol. - 1998. - Is. 3. - P. 351-355. - Cited References: 13 . - ISSN 0002-3329

РУБ Biology

Аннотация: The initial phase of formation of the biogenic suspension was studied in experimental communities of bacteria and protozoans that simulate degradation of the phenol technogenic flows. Protozoans were shown to initiate formation of bacterial aggregates and increase the size of bacterial flocules several hundred times. Factors were found by the methods of mathematical planning, which markedly affect the size and amount of flocules. Unlike natural water bodies, in the sample communites, the presence of abiogenic substrate was not essential for aggregation of the bacteria, The aggregated bacterial cells make an important contribution to degradation of organic compounds.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gurevich, Y.L.; Teremova, M.I.; Latyntseva, A.V.

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On the applicability of nanodiamonds produced by detonation synthesis for phenol testing in aqueous media / N. O. Ronzhin, A. P. Puzyr, V. S. Bondar // Dokl. Chem. - 2017. - Vol. 475, Is. 1. - P155-158, DOI 10.1134/S0012500817070011 . - ISSN 0012-5008
Аннотация: It was found that the catalytic effect of modified nanodiamonds (MND) in the H2O2–4-aminoantipyrine–phenol oxidative azo coupling reaction is due to microimpurities of iron and copper ions on the surface of nanoparticles. The efficiency of MND as a catalyst is determined by the amount of surface impurities of these ions and can be doubled by their additional adsorption on nanoparticles. Using MND for phenol indication ensures a linear yield of the colored product of the azo coupling reaction over an analyte concentration range of 0.05–10 ?g/mL. The possibility of reusing MND for phenol testing in aqueous samples was demonstrated. © 2017, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ronzhin, N. O.; Puzyr, A. P.; Bondar, V. S.

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Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications / O. Mogilnaya, N. Ronzhin, E. Posokhina, V. Bondar // Protein J. - 2021, DOI 10.1007/s10930-021-10010-z. - Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, Project No. 0356-2019-0022. . - Article in press. - ISSN 1572-3887. - ISSN 1573-4943

РУБ Biochemistry & Molecular Biology
Рубрики:
ARYL-ALCOHOL OXIDASE
   GLUCOSE-OXIDASE
   PEROXIDASES
   MECHANISM
Кл.слова (ненормированные):
Extracellular oxidase -- Flavoprotein -- Fungi -- Phenol
Аннотация: The extracellular enzyme with oxidase function was extracted from the Neonothopanus nambi luminescent fungus by using mild processing of mycelium with beta-glucosidase and then isolated by gel-filtration chromatography. The extracted enzyme is found to be a FAD-containing protein, catalyzing phenol co-oxidation with 4-aminoantipyrine without addition of H2O2, which distinguishes it from peroxidases. This fact allowed us to assume that this enzyme may be a mixed-function oxidase. According to gel-filtration chromatography and SDS-PAGE, the oxidase has molecular weight of 60 kDa. The enzyme exhibits maximum activity at 55-70 degrees C and pH 5.0. Kinetic parameters K-m and V-max of the oxidase for phenol were 0.21 mM and 0.40 mu M min(-1). We suggest that the extracted enzyme can be useful to develop a simplified biosensor for colorimetric detection of phenol in aqueous media, which does not require using hydrogen peroxide.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Mogilnaya, Olga; Ronzhin, Nikita; Posokhina, Ekaterina; Bondar, Vladimir; [0356-2019-0022]

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Reusable System for Phenol Detection in an Aqueous Medium Based on Nanodiamonds and Extracellular Oxidase from Basidiomycete Neonothopanus nambi / N. O. Ronzhin, O. A. Mogilnaya, E. D. Posokhina, V. S. Bondar // Dokl. Biochem. Biophys. - 2021. - Vol. 499, Is. 1. - P220-224, DOI 10.1134/S1607672921040141. - Cited References:15 . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
PEROXIDASES
EXPRESSION
Кл.слова (ненормированные):
nanodiamond -- extracellular oxidase -- basidiomycete Neonothopanus nambi -- indication system -- phenol
Аннотация: A reusable system for phenol determination in an aqueous medium was obtained by adsorption of extracellular oxidase from fungus Neonothopanus nambi onto modified nanodiamonds (MND) synthesized by detonation. It was found that the enzyme strongly binds to MND and exhibits catalytic activity in the reaction of co-oxidation of phenol with 4-aminoantipyrine without the addition of hydrogen peroxide. In the presence of the MND-oxidase complex, a significantly (by an order of magnitude) higher yield of the reaction product is recorded as compared to the yield in the presence of a free enzyme; the mechanism of the revealed effect is discussed. Model experiments have demonstrated the multiple use of the MND-oxidase complex for testing phenol in aqueous samples. The immobilized enzyme exhibits functional activity during long-term (2 months) storage of the MND-oxidase complex at 4 degrees C. The data obtained create the prerequisites for using the created system in environmental monitoring of water pollution with phenol.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys,Fed Res Ctr, Krasnoyarsk, Russia.

Доп.точки доступа:
Ronzhin, N. O.; Mogilnaya, O. A.; Posokhina, E. D.; Bondar, V. S.

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A new composite material based on alumina nanofibers and detonation nanodiamonds: synthesis, characterization, and sensing application / N. O. Ronzhin, E. D. Posokhina, E. V. Mikhlina [et al.] // J. Nanopart. Res. - 2021. - Vol. 23, Is. 9. - Ст. 199, DOI 10.1007/s11051-021-05309-y. - Cited References:57. - This work is partially supported by the Russian Foundation for Basic Research, Project 18-29-19078 (E. V. Mikhlina, M. M. Simunin, I. Ryzhkov). . - ISSN 1388-0764. - ISSN 1572-896X

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
ELECTROCHEMICAL ENERGY-STORAGE
SELECTIVE DETECTION
PHENOL DETECTION
Кл.слова (ненормированные):
Nanodiamonds -- Alumina nanofibers -- Composite -- Indicator system -- Phenol
Аннотация: The development of inexpensive, easy-to-produce, and easy-to-use analytical tools for detection of harmful and toxic substances is a relevant research problem with direct applications in environmental monitoring and protection. In this work, we propose a novel composite material based on alumina nanofibers and detonation nanodiamonds for detection of phenol in aqueous medium. The composite material was obtained by mixing an aqueous suspension of alumina nanofibers with a diameter of 10-15 nm and a length of several microns and a hydrosol of nanodiamonds with an average cluster size of 70 nm. The mechanisms underlying the interaction of these nanomaterials are clarified and the physicochemical properties of the composite are investigated. The SEM and TEM studies show that the obtained composite has a network structure, in which clusters of nanodiamonds (10-20 nm in diameter) are distributed over the surface of nanofibers. Coupling of nanomaterials occurs due to opposite signs of their zeta potentials, which results in electrostatic attraction and subsequent chemical bonding as indicated by the X-ray photoelectron spectroscopy and simultaneous thermal analysis. The bonding apparently occurs between functional groups (mainly carboxyl) on the surface of nanodiamonds and amphoteric hydroxyl groups on the surface of alumina nanofibers. The proposed composite allows an easy-to-perform colorimetric analysis for qualitative and quantitative determination of phenol in aqueous samples with linear response over a wide range of concentrations (0.5-106 mu M). Multiple tests have shown that the composite is reusable and retains its catalytic function for at least 1 year during storage at room temperature.

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Держатели документа:
Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny 79, Krasnoyarsk 660041, Russia.
Inst Computat Modelling SB RAS, Akademgorodok 50-44, Krasnoyarsk 660036, Russia.
Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia.
Fed Res Ctr KSC SB RAS, Akademgorodok 50-38, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ronzhin, Nikita O.; Posokhina, Ekaterina D.; Mikhlina, Elena, V; Mikhlin, Yuri L.; Simunin, Mikhail M.; Tarasova, Lyudmila S.; Vorobyev, Sergey A.; Bondar, Vladimir S.; Ryzhkov, Ilya I.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078]

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10.

Production of a Composite Based on Alumina Nanofibers and Detonation Nanodiamonds for Creating Phenol Indication Systems / N. O. Ronzhin, E. D. Posokhina, E. V. Mikhlina [et al.] // Dokl. Chem. - 2019. - Vol. 489, Is. 1. - P267-271, DOI 10.1134/S001250081911003X . - ISSN 0012-5008
Аннотация: Abstract: A composite of alumina nanofibers (ANF) and modified detonation nanodiamonds (MDND) was produced by mixing aqueous suspensions of the components in a weight ratio of 5 : 1 with subsequent incubation of the mixture for 15 min at 32°C. It was assumed that the formation of the composite is ensured by the difference of the zeta potentials of the components, which is negative for MDND and positive for ANF. Vacuum filtration of the mixture through a fluoroplastic filter (pore diameter 0.6 ?m) formed disks 40 mm in diameter, which were then heat-treated at 300°C to impart structural stability to the composite. Scanning electron microscopy detected that the obtained composite has a network structure, in which MDND particles are distributed over the surface of ANF. It was determined that the MDND particles incorporated in the composite catalyze the phenol–4-aminoantipyrine–H2O2 oxidative azo coupling reaction to form a colored product (quinoneimine). The applicability of the composite to repeated phenol detection in aqueous samples was demonstrated. © 2019, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, AkademgorodokKrasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Computational Modeling, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, AkademgorodokKrasnoyarsk, 660036, Russian Federation
Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, AkademgorodokKrasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Ronzhin, N. O.; Posokhina, E. D.; Mikhlina, E. V.; Simunin, M. M.; Nemtsev, I. V.; Ryzhkov, I. I.; Bondar, V. S.

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11.

РУБ Chemistry, Multidisciplinary
Рубрики:
NANOPARTICLES
GRAPHENE
Аннотация: A composite of alumina nanofibers (ANF) and modified detonation nanodiamonds (MDND) was produced by mixing aqueous suspensions of the components in a weight ratio of 5 : 1 with subsequent incubation of the mixture for 15 min at 32 degrees C. It was assumed that the formation of the composite is ensured by the difference of the zeta potentials of the components, which is negative for MDND and positive for ANF. Vacuum filtration of the mixture through a fluoroplastic filter (pore diameter 0.6 mu m) formed disks 40 mm in diameter, which were then heat-treated at 300 degrees C to impart structural stability to the composite. Scanning electron microscopy detected that the obtained composite has a network structure, in which MDND particles are distributed over the surface of ANF. It was determined that the MDND particles incorporated in the composite catalyze the phenol-4-aminoantipyrine-H2O2 oxidative azo coupling reaction to form a colored product (quinoneimine). The applicability of the composite to repeated phenol detection in aqueous samples was demonstrated.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Siberian Branch, Inst Computat Modeling, Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ronzhin, N. O.; Posokhina, E. D.; Mikhlina, E. V.; Simunin, M. M.; Nemtsev, I. V.; Ryzhkov, I. I.; Bondar, V. S.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078 mk]

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12.

Extracellular Oxidases of Basidiomycete Neonothopanus nambi: Isolation and Some Properties / N. O. Ronzhin, O. A. Mogilnaya, K. S. Artemenko [et al.] // Dokl. Biochem. Biophys. - 2020. - Vol. 490, Is. 1. - P38-42, DOI 10.1134/S1607672920010135. - Cited References:15 . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
PEROXIDASE-ACTIVITY
LIGHT-EMISSION
Кл.слова (ненормированные):
extracellular oxidases -- basidiomycete Neonothopanus nambi -- beta-glucosidase -- gel-filtration chromatography -- veratryl alcohol -- phenol -- FAD
Аннотация: Using the original technique of treating biomass with beta-glucosidase, a pool of extracellular fungal enzymes was obtained for the first time from the mycelium of basidiomycete Neonothopanus nambi. Two protein fractions containing enzymes with oxidase activity were isolated from the extract by gel-filtration chromatography and conventionally called F1 and F2. Enzyme F1 has a native molecular weight of 80-85 kDa and does not contain chromophore components; however, it catalyzes the oxidation of veratryl alcohol with K-m = 0.52 mM. Probably, this enzyme is an alcohol oxidase. Enzyme F2 with a native molecular weight of approximately 60 kDa is a FAD-containing protein. It catalyzes the cooxidation of phenol with 4-aminoantipyrine without the addition of exogenous hydrogen peroxide, which distinguishes it from the known peroxidases. It was assumed that this enzyme may be a mixed-function oxidase. F2 oxidase has K-m value 0.27 mM for phenol. The temperature optimums for oxidases F1 and F2 are 22-35 and 55-70 degrees C, and pH optimums are 6 and 5, respectively.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci, Inst Biophys,Fed Res Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Ronzhin, N. O.; Mogilnaya, O. A.; Artemenko, K. S.; Posokhina, E. D.; Bondar, V. S.

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13.

SEASONAL DYNAMICS OF THE KINETICS OF NATURAL PHENOL DECONTAMINATION IN THE SYDIN BAY OF THE KRASNOYARSK WATER RESERVOIR [Текст] / M. I. GLADYSHEV, I. V. GRIBOVSKAIA, L. A. SHCHUR // DOKLADY AKADEMII NAUK SSSR. - 1990. - Vol. 313, Is. 6. - P. 1512-1514. - Cited References: 4 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
GLADYSHEV, M.I.; GRIBOVSKAIA, I.V.; SHCHUR, L.A.

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14.

A new scheme of lignin biosynthesis and the mechanism of its regulation of functional properties. / D. A. Semenov, N. E. Sudachkova, R. G. Khlebopros // Doklady. Biochemistry and biophysics. - 2002. - Vol. 382. - P50-52 . - ISSN 1607-6729
Кл.слова (ненормированные):
lignin -- peroxidase -- phenol derivative -- polymer -- article -- biosynthesis -- cell wall -- chemistry -- metabolism -- oxidation reduction reaction -- physiology -- plant -- Cell Wall -- Lignin -- Oxidation-Reduction -- Peroxidase -- Phenols -- Plants -- Polymers

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Semenov, D.A.; Sudachkova, N.E.; Khlebopros, R.G.

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15.

Comparative study of PHA degradation in natural reservoirs having various types of ecosystems [Text] / O. . Voinova, M. I. Gladyshev, T. G. Volova // Macromol. Symp. - 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - P34-37, DOI 10.1002/masy.zoo850906. - Cited References: 15 . - 4. - ISSN 1022-1360

РУБ Polymer Science
Рубрики:
POLYHYDROXYALKANOATES
   BIODEGRADATION
   DISAPPEARANCE
   POLYESTERS
   PHENOL
Кл.слова (ненормированные):
biodegradable polyesters -- ecosystem -- natural environment -- PHA -- water reservoir
Аннотация: The kinetics of polyhydroxyalkanoate (PHA) degradation in natural environment in two water reservoirs having various ecological characteristics was studied. It was shown that biodegradation of polymer essentially depends on the environment temperature and inorganic composition of water. The processes of polymer degradation under aerobic and anaerobic conditions were compared. The polymer degradation was slower under anaerobic conditions.

Держатели документа:
[Voinova, Olga
Gladyshev, Michail I
Volova, Tatiana G.] Inst Biophys SB RAS, Akad Gorodok, Krasnoyarsk 660036, Russia
[Gladyshev, Michail I
Volova, Tatiana G.] Siberian Fed Natl Univ, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Voinova, O...; Gladyshev, M.I.; Volova, T.G.

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16.

Violet bioluminescence and fast kinetics from W92F obelin: Structure-based proposals for the bioluminescence triggering and the identification of the emitting species [Text] / E. S. Vysotski [et al.] // Biochemistry. - 2003. - Vol. 42, Is. 20. - P6013-6024, DOI 10.1021/bi027258h. - Cited References: 45 . - ISSN 0006-2960

РУБ Biochemistry & Molecular Biology
Рубрики:
RAY CRYSTALLOGRAPHIC ANALYSIS
   PHOTOPROTEIN AEQUORIN
   ANGSTROM RESOLUTION
   RECOMBINANT OBELIN
   CALCIUM
   LUMINESCENCE
   LONGISSIMA
   EVOLUTION
   PROTEINS
   COELENTERAZINE
Аннотация: Obelin from the hydroid Obelia longissima and aequorin are members of a subfamily of Ca2+-regulated photoproteins that is a part of the larger EF-hand calcium binding protein family. On the addition of Ca2+, obelin generates a blue bioluminescence emission (lambda(max) = 485 nm) as the result of the oxidative decarboxylation of the bound substrate, coelenterazine. The W92F obelin mutant is noteworthy because of the unusually high speed with which it responds to sudden changes of [Ca2+] and because it emits violet light rather than blue due to a prominent band with lambda(max) = 405 nm. Increase of pH in the range from 5.5 to 8.5 and using D2O both diminish the contribution of the 405 nm band, indicating that excited state proton transfer is involved. Fluorescence model studies have suggested the origin of the 485 nm emission as the excited state of an anion of coelenteramide, the bioluminescence reaction product, and 405 nm from the excited neutral state. Assuming that the dimensions of the substrate binding cavity do not change during the excited state formation, a His22 residue within hydrogen bonding distance to the 6-(p-hydroxy)-phenyl group of the excited coelenteramide is a likely candidate for accepting the phenol proton to produce an ion-pair excited state, in support of recent suggestions for the bioluminescence emitting state. The proton transfer could be impeded by removal of the Trp92 H-bond, resulting in strong enhancement of a 405 nm band giving the violet color of bioluminescence. Comparative analysis of 3D structures of the wild-type (WT) and W92F obelins reveals that there are structural displacements of certain key Ca2+-ligating residues in the loops of the two C-terminal EF hands as well as clear differences in hydrogen bond networks in W92F. For instance, the hydrogen bond between the side-chain oxygen atom of Asp 169 and the main-chain nitrogen of Arg112 binds together the incoming alpha-helix of loop III and the exiting cc-helix of loop IV in WT, providing probably concerted changes in these EF hands on calcium binding. But this linkage is not found in W92F obelin. These differences apparently do not change the overall affinity to calcium of W92F obelin but may account for the kinetic differences between the WT and mutant obelins. From analysis of the hydrogen bond network in the coelenterazine binding cavity, it is proposed that the trigger for bioluminescence reaction in these Ca2+-regulated photoproteins may be a shift of the hydrogen bond donor-acceptor separations around the coelenterazine-2-hydroperoxy substrate, initiated by small spatial adjustment of the exiting a-helix of loop IV.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Univ Georgia, Dept Chem, Athens, GA USA
RAS, SB, Photobiol Lab, Inst Biophys, Krasnoyarsk, Russia
Univ Washington, Friday Harbor Labs, Seattle, WA 98195 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vysotski, E.S.; Liu, Z.J.; Markova, S.V.; Blinks, J.R.; Deng, L...; Frank, L.A.; Herko, M...; Malikova, N.P.; Rose, J.P.; Wang, B.C.; Lee, J...

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17.

Influence of anthropogenic pollution on content of essential polyunsaturated fatty acids in links of food chain of river ecosystem / M. I. Gladyshev [et al.] // Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - P376-385, DOI 10.1134/S1995425512040051 . - ISSN 1995-4255
Кл.слова (ненормированные):
gammarus -- grayling -- heavy metals -- polyunsaturated fatty acids -- amphipod -- anthropogenic effect -- anthropogenic source -- fatty acid -- food chain -- heavy metal -- littoral environment -- oil pollution -- phenol -- river system -- salmonid -- sampling -- Krasnoyarsk [Russian Federation] -- Russian Federation -- Yenisei River -- Gammarus
Аннотация: In the course of monthly sampling in 2008-2010, two regions of the littoral of the Yenisei river were compared. One of these regions (conventionally pure) was situated upstream of Krasnoyarsk, while the other (conventionally polluted) was downstream of Krasnoyarsk. The concentrations of heavy metals, oil products, phenols, biogenic elements and essential polyunsaturated fatty acids (PUFAs) in various components of the river ecosystem were determined. It was discovered that the anthropogenic pollution causes a decrease in the resources of essential PUFA in the biomass of the upper links of the food chain of the river ecosystem. В© 2012 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnii prosp. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Anishchenko, O.V.; Sushchnik, N.N.; Kalacheva, G.S.; Gribovskaya, I.V.; Ageev, A.V.

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18.

The effect of algal blooms on the disappearance of phenol in a small forest pond / M. I. Gladyshev [et al.] // Water Research. - 1998. - Vol. 32, Is. 9. - P2769-2775, DOI 10.1016/S0043-1354(98)00009-8 . - ISSN 0043-1354
Кл.слова (ненормированные):
Algal blooms -- Phenol -- Seasonal dynamics of biodegradation -- Self-purification -- Algae -- Biodegradation -- Ecosystems -- Phenols -- Purification -- Reaction kinetics -- Reservoirs (water) -- Surface waters -- Experimental microecosystems -- Forest pond waters -- Green algae Volvox aureus -- Inorganic nutrients -- Krasnoyarsk reservoir -- Water pollution -- lake water -- phenol -- article -- ecosystem -- forest -- green alga -- priority journal -- russian federation -- water pollutant -- water temperature
Аннотация: Using experimental microecosystems the kinetics of phenol disappearance in small forest pond waters (Siberia, Russia) in the summer of 1995-96 were investigated. Despite of high variability of components of the ecosystem (plankton biomass and species composition) and two pronounced 'blooms' of green algae Volvox aureus the same kinetics of the disappearance took place over the investigated period. Half-lives of the pollutant depended on water temperature only. A comparison of the self-purification of the pond with that of the Krasnoyarsk reservoir, 'blooming' with blue-greens was carried out. Half-lives in the pond were significantly lower than that in the reservoir. During the periods of 'blooms' of the green algae in the pond the concentrations of inorganic nutrients were comparatively high and the phenol-degrading bacteria likely were not limited by these nutrients, in contrast to the periods of 'bloom' of the blue-green algae in the reservoir.

Scopus
Держатели документа:
Inst. Biophys. Siberian Br. Russ. A., Akademgorodok, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Kalachova, G.S.; Shchur, L.A.

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19.

Bioluminescent method in studying the complex effect of sewage components / D. I. Stom [et al.] // Archives of Environmental Contamination and Toxicology. - 1992. - Vol. 22, Is. 2. - P203-208 . - ISSN 0090-4341
Кл.слова (ненормированные):
heavy metal -- phenol derivative -- quinone derivative -- article -- bacterium -- bioluminescence -- cell membrane -- nonhuman -- priority journal -- sludge -- ultrastructure -- Benzoquinones -- Catechin -- Hydroquinones -- Luminescent Measurements -- Metals -- Phenol -- Phenols -- Photobacterium -- Sewage -- Vibrio -- Water Pollutants, Chemical -- Bacteria (microorganisms)
Аннотация: The inhibition of bacterial luminescence has been used in testing industrial enterprises sewage. The toxicity of the sewage is less than the total toxicity of separate components due to neutralization of quinone products of polyphenol oxidation in the reactions with the other phenol components of sewage. Toxicity increase is due to their influence on the cell membrane. Studies of cell ultrastructure confirm this fact. The studied mechanism of the complex effect allowed a more accurate forecast of the ecological situation during the discharge of phenol compounds and metals. It also showed the necessity of taking into account the complex effect of sewage components on contaminant discharge into water reservoirs.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Stom, D.I.; Geel, T.A.; Balayan, A.E.; Shachova, G.I.; Kuznetsov, A.M.; Medvedeva, S.E.

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20.

Composition of free fatty acids of the surface film of water and kinetic of self-purification from phenol in forest pond at ''bloom'' of Volvox aureus [Текст] / M. I. Gladyshev [и др.] // Dokl. Akad. Nauk. - 1996. - Vol. 349, Is. 6. - С. 840-843. - Cited References: 5 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
KRASNOYARSK RESERVOIR
MODELS
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Kalacheva, G.S.; Shchur, L.A.

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