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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin, Nikita, Puzyr, Alexey, Bondar, Vladimir
Заглавие : Detonation Nanodiamonds as a New Tool for Phenol Detection in Aqueous Medium
Колич.характеристики :6 с
Коллективы : Russian Academy of Sciences [0356-2016-0709]; Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology [16-43-243027]
Место публикации : J. Nanosci. Nanotechnol.: AMER SCIENTIFIC PUBLISHERS, 2018. - Vol. 18, Is. 8. - С. 5448-5453. - ISSN 1533-4880, DOI 10.1166/jnn.2018.15382. - ISSN 1533-4899(eISSN)
Примечания : Cited References:27. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (Project No. 0356-2016-0709) and Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund to the research (Project No. 16-43-243027).
Предметные рубрики: ENVIRONMENTAL-POLLUTANTS
DRUG-DELIVERY
PARTICLES
CARBON
Ключевые слова (''Своб.индексиров.''): detonation nanodiamonds--catalytic activity--metal ions--azo coupling--reaction--phenol detection
Аннотация: This paper demonstrates the effectiveness of using detonation nanodiamonds (DNDs) for detecting phenol in aqueous medium. The study has shown that the catalytic effect of DNDs in the oxidative azo coupling reaction (phenol-4-aminoantipyrine-hydrogen peroxide) is produced by trace amounts of iron and copper ions adsorbed on the surface of nanoparticles. The effectiveness of DNDs as a catalyst is determined by the amounts of these adsorbates and can be enhanced by a factor of two by additional adsorption of these ions onto the nanoparticles. A rise in the temperature of the ONO-catalyzed azo coupling reaction leads to a considerable (4.5-fold) increase in the reaction product yield. DNDs used to detect phenol in aqueous medium enable a linear increase in the yield of the product of the azo coupling reaction at concentrations of the analyte of between 0.05 and 10 mu g/mlThe study demonstrates that DNDs can be reused to detect phenol in water samples.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Artemenko K. S., Bondar V. S.
Заглавие : Creation of Bifunctional Indicating Complex Based on Nanodiamonds and Extracellular Oxidases of Luminous Fungus Neonothopanus nambi
Колич.характеристики :4 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2018. - Vol. 480, Is. 1. - С. 135-138. - ISSN 1607-6729, DOI 10.1134/S160767291803002X. - ISSN 1608-3091(eISSN)
Примечания : Cited References:13
Предметные рубрики: PHANEROCHAETE-CHRYSOSPORIUM
NANOPARTICLES
Аннотация: A bifunctional indicating complex was created by immobilization of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MNDs) synthesized by detonation. It was found that the enzymes firmly adsorb onto MND particles and exhibit their catalytic activity. Model in vitro experiments showed that the created MND-enzymes complex is suitable for repeated use for analyte (glucose and phenol) testing and retains its activity after storage at 4 degrees C in deionized water for 1 month. The data obtained offer the prospects for developing a new class of reusable multifunctional indicating and diagnostic test systems on the basis of MNDs and higher fungal enzymes for medical and ecological analytics.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya, Olga, Ronzhin, Nikita, Artemenko, Karina, Bondar, Vladimir
Заглавие : Nanodiamonds as an effective adsorbent for immobilization of extracellular peroxidases from luminous fungus Neonothopanus nambi to construct a phenol detection system
Колич.характеристики :9 с
Коллективы : Russian Academy of Sciences [0356-2016-0709]
Место публикации : Biocatal. Biotransform.: TAYLOR & FRANCIS LTD, 2019. - Vol. 37, Is. 2. - С. 97-105. - ISSN 1024-2422, DOI 10.1080/10242422.2018.1472586. - ISSN 1029-2446(eISSN)
Примечания : Cited References:50. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences [project no. 0356-2016-0709].
Предметные рубрики: CARBON NANOTUBES
ARMILLARIA-BOREALIS
LIGHT-EMISSION
DEGRADATION
Ключевые слова (''Своб.индексиров.''): nanodiamonds--immobilization--luminous fungus--beta-glucosidase--peroxidase--indicator system
Аннотация: Modified nanodiamonds (MNDs) produced by detonation synthesis can be used as an effective adsorbent to immobilize extracellular peroxidases of the luminous basidiomycete Neonothopanus nambi. The enzymes are firmly immobilized on MND particles and exhibit catalytic activity. The indicator system (the MND-enzyme complex) reused many times retains its ability to catalyze reaction of co-oxidation of phenol and 4-aminoantipirine in the presence of hydrogen peroxide and remains functionally active during long-term storage (for 1 month or longer) in aqueous suspensions at 4 degrees C. MNDs and enzymes of higher fungi can be effectively used to construct new reusable indicator systems for analytical applications such as monitoring contamination of aquatic environments by phenolic compounds.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : GLADYSHEV M.I., GRIBOVSKAYA I.V.
Заглавие : PHENOL BIODEGRADATION IN THE YENISEI RIVER AND THE KRASNOYARSK RESERVOIR, RUSSIA
Колич.характеристики :5 с
Место публикации : HYDROLOGICAL, CHEMICAL AND BIOLOGICAL PROCESSES OF TRANSFORMATION AND TRANSPORT OF CONTAMINANTS IN AQUATIC ENVIRONMENTS. Ser. IAHS PUBLICATIONS: INT ASSOC HYDROLOGICAL SCIENCES, 1994. - Hydrochemistry 1993 Symposium on Hydrological, Chemical and Biological Processes of Transformation and Transport of Contaminants in Aquatic Environments (MAY 24-28, 1993, ROSTOV NA DONU, RUSSIA), Is. 219. - P217-221. - ISBN 0144-7815. - ISBN 0-947571-88-4
Примечания : Cited References: 0
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gurevich Y.L., Teremova M.I., Latyntseva A.V.
Заглавие : Flocculation of bacterial cells in a culture with protozoans
Колич.характеристики :5 с
Место публикации : Izv. Akad. Nauk Ser. Biol.: MEZHDUNARODNAYA KNIGA, 1998. - Is. 3. - P351-355. - ISSN 0002-3329
Примечания : Cited References: 13
Аннотация: The initial phase of formation of the biogenic suspension was studied in experimental communities of bacteria and protozoans that simulate degradation of the phenol technogenic flows. Protozoans were shown to initiate formation of bacterial aggregates and increase the size of bacterial flocules several hundred times. Factors were found by the methods of mathematical planning, which markedly affect the size and amount of flocules. Unlike natural water bodies, in the sample communites, the presence of abiogenic substrate was not essential for aggregation of the bacteria, The aggregated bacterial cells make an important contribution to degradation of organic compounds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Puzyr A. P., Bondar V. S.
Заглавие : On the applicability of nanodiamonds produced by detonation synthesis for phenol testing in aqueous media
Место публикации : Dokl. Chem.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 475, Is. 1. - С. 155-158. - ISSN 00125008 (ISSN) , DOI 10.1134/S0012500817070011
Аннотация: It was found that the catalytic effect of modified nanodiamonds (MND) in the H2O2–4-aminoantipyrine–phenol oxidative azo coupling reaction is due to microimpurities of iron and copper ions on the surface of nanoparticles. The efficiency of MND as a catalyst is determined by the amount of surface impurities of these ions and can be doubled by their additional adsorption on nanoparticles. Using MND for phenol indication ensures a linear yield of the colored product of the azo coupling reaction over an analyte concentration range of 0.05–10 ?g/mL. The possibility of reusing MND for phenol testing in aqueous samples was demonstrated. © 2017, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya, Olga, Ronzhin, Nikita, Posokhina, Ekaterina, Bondar, Vladimir
Заглавие : Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications
Колич.характеристики :10 с
Коллективы : [0356-2019-0022]
Место публикации : Protein J.: SPRINGER, 2021. - Article in press. - ISSN 1572-3887, DOI 10.1007/s10930-021-10010-z. - ISSN 1573-4943(eISSN)
Примечания : Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, Project No. 0356-2019-0022.
Предметные рубрики: ARYL-ALCOHOL OXIDASE
GLUCOSE-OXIDASE
PEROXIDASES
MECHANISM
Аннотация: The extracellular enzyme with oxidase function was extracted from the Neonothopanus nambi luminescent fungus by using mild processing of mycelium with beta-glucosidase and then isolated by gel-filtration chromatography. The extracted enzyme is found to be a FAD-containing protein, catalyzing phenol co-oxidation with 4-aminoantipyrine without addition of H2O2, which distinguishes it from peroxidases. This fact allowed us to assume that this enzyme may be a mixed-function oxidase. According to gel-filtration chromatography and SDS-PAGE, the oxidase has molecular weight of 60 kDa. The enzyme exhibits maximum activity at 55-70 degrees C and pH 5.0. Kinetic parameters K-m and V-max of the oxidase for phenol were 0.21 mM and 0.40 mu M min(-1). We suggest that the extracted enzyme can be useful to develop a simplified biosensor for colorimetric detection of phenol in aqueous media, which does not require using hydrogen peroxide.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Mogilnaya O. A., Posokhina E. D., Bondar V. S.
Заглавие : Reusable System for Phenol Detection in an Aqueous Medium Based on Nanodiamonds and Extracellular Oxidase from Basidiomycete Neonothopanus nambi
Колич.характеристики :5 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 499, Is. 1. - С. 220-224. - ISSN 1607-6729, DOI 10.1134/S1607672921040141. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15
Предметные рубрики: PEROXIDASES
EXPRESSION
Аннотация: A reusable system for phenol determination in an aqueous medium was obtained by adsorption of extracellular oxidase from fungus Neonothopanus nambi onto modified nanodiamonds (MND) synthesized by detonation. It was found that the enzyme strongly binds to MND and exhibits catalytic activity in the reaction of co-oxidation of phenol with 4-aminoantipyrine without the addition of hydrogen peroxide. In the presence of the MND-oxidase complex, a significantly (by an order of magnitude) higher yield of the reaction product is recorded as compared to the yield in the presence of a free enzyme; the mechanism of the revealed effect is discussed. Model experiments have demonstrated the multiple use of the MND-oxidase complex for testing phenol in aqueous samples. The immobilized enzyme exhibits functional activity during long-term (2 months) storage of the MND-oxidase complex at 4 degrees C. The data obtained create the prerequisites for using the created system in environmental monitoring of water pollution with phenol.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin, Nikita O., Posokhina, Ekaterina D., Mikhlina, Elena, V, Mikhlin, Yuri L., Simunin, Mikhail M., Tarasova, Lyudmila S., Vorobyev, Sergey A., Bondar, Vladimir S., Ryzhkov, Ilya I.
Заглавие : A new composite material based on alumina nanofibers and detonation nanodiamonds: synthesis, characterization, and sensing application
Колич.характеристики :14 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078]
Место публикации : J. Nanopart. Res.: SPRINGER, 2021. - Vol. 23, Is. 9. - Ст.199. - ISSN 1388-0764, DOI 10.1007/s11051-021-05309-y. - ISSN 1572-896X(eISSN)
Примечания : Cited References:57. - This work is partially supported by the Russian Foundation for Basic Research, Project 18-29-19078 (E. V. Mikhlina, M. M. Simunin, I. Ryzhkov).
Предметные рубрики: ELECTROCHEMICAL ENERGY-STORAGE
SELECTIVE DETECTION
PHENOL DETECTION
Аннотация: The development of inexpensive, easy-to-produce, and easy-to-use analytical tools for detection of harmful and toxic substances is a relevant research problem with direct applications in environmental monitoring and protection. In this work, we propose a novel composite material based on alumina nanofibers and detonation nanodiamonds for detection of phenol in aqueous medium. The composite material was obtained by mixing an aqueous suspension of alumina nanofibers with a diameter of 10-15 nm and a length of several microns and a hydrosol of nanodiamonds with an average cluster size of 70 nm. The mechanisms underlying the interaction of these nanomaterials are clarified and the physicochemical properties of the composite are investigated. The SEM and TEM studies show that the obtained composite has a network structure, in which clusters of nanodiamonds (10-20 nm in diameter) are distributed over the surface of nanofibers. Coupling of nanomaterials occurs due to opposite signs of their zeta potentials, which results in electrostatic attraction and subsequent chemical bonding as indicated by the X-ray photoelectron spectroscopy and simultaneous thermal analysis. The bonding apparently occurs between functional groups (mainly carboxyl) on the surface of nanodiamonds and amphoteric hydroxyl groups on the surface of alumina nanofibers. The proposed composite allows an easy-to-perform colorimetric analysis for qualitative and quantitative determination of phenol in aqueous samples with linear response over a wide range of concentrations (0.5-106 mu M). Multiple tests have shown that the composite is reusable and retains its catalytic function for at least 1 year during storage at room temperature.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Posokhina E. D., Mikhlina E. V., Simunin M. M., Nemtsev I. V., Ryzhkov I. I., Bondar V. S.
Заглавие : Production of a Composite Based on Alumina Nanofibers and Detonation Nanodiamonds for Creating Phenol Indication Systems
Место публикации : Dokl. Chem.: Pleiades Publishing, 2019. - Vol. 489, Is. 1. - С. 267-271. - ISSN 00125008 (ISSN), DOI 10.1134/S001250081911003X
Аннотация: Abstract: A composite of alumina nanofibers (ANF) and modified detonation nanodiamonds (MDND) was produced by mixing aqueous suspensions of the components in a weight ratio of 5 : 1 with subsequent incubation of the mixture for 15 min at 32°C. It was assumed that the formation of the composite is ensured by the difference of the zeta potentials of the components, which is negative for MDND and positive for ANF. Vacuum filtration of the mixture through a fluoroplastic filter (pore diameter 0.6 ?m) formed disks 40 mm in diameter, which were then heat-treated at 300°C to impart structural stability to the composite. Scanning electron microscopy detected that the obtained composite has a network structure, in which MDND particles are distributed over the surface of ANF. It was determined that the MDND particles incorporated in the composite catalyze the phenol–4-aminoantipyrine–H2O2 oxidative azo coupling reaction to form a colored product (quinoneimine). The applicability of the composite to repeated phenol detection in aqueous samples was demonstrated. © 2019, Pleiades Publishing, Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Posokhina E. D., Mikhlina E. V., Simunin M. M., Nemtsev I. V., Ryzhkov I. I., Bondar V. S.
Заглавие : Production of a Composite Based on Alumina Nanofibers and Detonation Nanodiamonds for Creating Phenol Indication Systems
Колич.характеристики :5 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078 mk]
Место публикации : Dokl. Chem.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 489. - С. 267-271. - ISSN 0012-5008, DOI 10.1134/S001250081911003X. - ISSN 1608-3113(eISSN)
Примечания : Cited References:13. - This work was supported by the Russian Foundation for Basic Research (project no. 18-29-19078 mk).
Предметные рубрики: NANOPARTICLES
GRAPHENE
Аннотация: A composite of alumina nanofibers (ANF) and modified detonation nanodiamonds (MDND) was produced by mixing aqueous suspensions of the components in a weight ratio of 5 : 1 with subsequent incubation of the mixture for 15 min at 32 degrees C. It was assumed that the formation of the composite is ensured by the difference of the zeta potentials of the components, which is negative for MDND and positive for ANF. Vacuum filtration of the mixture through a fluoroplastic filter (pore diameter 0.6 mu m) formed disks 40 mm in diameter, which were then heat-treated at 300 degrees C to impart structural stability to the composite. Scanning electron microscopy detected that the obtained composite has a network structure, in which MDND particles are distributed over the surface of ANF. It was determined that the MDND particles incorporated in the composite catalyze the phenol-4-aminoantipyrine-H2O2 oxidative azo coupling reaction to form a colored product (quinoneimine). The applicability of the composite to repeated phenol detection in aqueous samples was demonstrated.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin N. O., Mogilnaya O. A., Artemenko K. S., Posokhina E. D., Bondar V. S.
Заглавие : Extracellular Oxidases of Basidiomycete Neonothopanus nambi: Isolation and Some Properties
Колич.характеристики :5 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 490, Is. 1. - С. 38-42. - ISSN 1607-6729, DOI 10.1134/S1607672920010135. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15
Предметные рубрики: PEROXIDASE-ACTIVITY
LIGHT-EMISSION
Аннотация: Using the original technique of treating biomass with beta-glucosidase, a pool of extracellular fungal enzymes was obtained for the first time from the mycelium of basidiomycete Neonothopanus nambi. Two protein fractions containing enzymes with oxidase activity were isolated from the extract by gel-filtration chromatography and conventionally called F1 and F2. Enzyme F1 has a native molecular weight of 80-85 kDa and does not contain chromophore components; however, it catalyzes the oxidation of veratryl alcohol with K-m = 0.52 mM. Probably, this enzyme is an alcohol oxidase. Enzyme F2 with a native molecular weight of approximately 60 kDa is a FAD-containing protein. It catalyzes the cooxidation of phenol with 4-aminoantipyrine without the addition of exogenous hydrogen peroxide, which distinguishes it from the known peroxidases. It was assumed that this enzyme may be a mixed-function oxidase. F2 oxidase has K-m value 0.27 mM for phenol. The temperature optimums for oxidases F1 and F2 are 22-35 and 55-70 degrees C, and pH optimums are 6 and 5, respectively.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GLADYSHEV M.I., GRIBOVSKAIA I.V., SHCHUR L.A.
Заглавие : SEASONAL DYNAMICS OF THE KINETICS OF NATURAL PHENOL DECONTAMINATION IN THE SYDIN BAY OF THE KRASNOYARSK WATER RESERVOIR
Колич.характеристики :3 с
Место публикации : DOKLADY AKADEMII NAUK SSSR: MEZHDUNARODNAYA KNIGA, 1990. - Vol. 313, Is. 6. - P1512-1514. - ISSN 0002-3264
Примечания : Cited References: 4
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Semenov D.A., Sudachkova N.E., Khlebopros R.G.
Заглавие : A new scheme of lignin biosynthesis and the mechanism of its regulation of functional properties.
Место публикации : Doklady. Biochemistry and biophysics. - 2002. - Vol. 382. - С. 50-52. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): lignin--peroxidase--phenol derivative--polymer--article--biosynthesis--cell wall--chemistry--metabolism--oxidation reduction reaction--physiology--plant--cell wall--lignin--oxidation-reduction--peroxidase--phenols--plants--polymers
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Voinova O..., Gladyshev M.I., Volova T.G.
Заглавие : Comparative study of PHA degradation in natural reservoirs having various types of ecosystems
Место публикации : Macromol. Symp.: WILEY-V C H VERLAG GMBH, 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - С. 34-37. - 4. - ISSN 1022-1360, DOI 10.1002/masy.zoo850906
Примечания : Cited References: 15
Предметные рубрики: POLYHYDROXYALKANOATES
BIODEGRADATION
DISAPPEARANCE
POLYESTERS
PHENOL
Ключевые слова (''Своб.индексиров.''): biodegradable polyesters--ecosystem--natural environment--pha--water reservoir
Аннотация: The kinetics of polyhydroxyalkanoate (PHA) degradation in natural environment in two water reservoirs having various ecological characteristics was studied. It was shown that biodegradation of polymer essentially depends on the environment temperature and inorganic composition of water. The processes of polymer degradation under aerobic and anaerobic conditions were compared. The polymer degradation was slower under anaerobic conditions.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotski E.S., Liu Z.J., Markova S.V., Blinks J.R., Deng L..., Frank L.A., Herko M..., Malikova N.P., Rose J.P., Wang B.C., Lee J...
Заглавие : Violet bioluminescence and fast kinetics from W92F obelin: Structure-based proposals for the bioluminescence triggering and the identification of the emitting species
Колич.характеристики :12 с
Место публикации : Biochemistry: AMER CHEMICAL SOC, 2003. - Vol. 42, Is. 20. - С. 6013-6024. - ISSN 0006-2960, DOI 10.1021/bi027258h
Примечания : Cited References: 45
Предметные рубрики: RAY CRYSTALLOGRAPHIC ANALYSIS
PHOTOPROTEIN AEQUORIN
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
CALCIUM
LUMINESCENCE
LONGISSIMA
EVOLUTION
PROTEINS
COELENTERAZINE
Аннотация: Obelin from the hydroid Obelia longissima and aequorin are members of a subfamily of Ca2+-regulated photoproteins that is a part of the larger EF-hand calcium binding protein family. On the addition of Ca2+, obelin generates a blue bioluminescence emission (lambda(max) = 485 nm) as the result of the oxidative decarboxylation of the bound substrate, coelenterazine. The W92F obelin mutant is noteworthy because of the unusually high speed with which it responds to sudden changes of [Ca2+] and because it emits violet light rather than blue due to a prominent band with lambda(max) = 405 nm. Increase of pH in the range from 5.5 to 8.5 and using D2O both diminish the contribution of the 405 nm band, indicating that excited state proton transfer is involved. Fluorescence model studies have suggested the origin of the 485 nm emission as the excited state of an anion of coelenteramide, the bioluminescence reaction product, and 405 nm from the excited neutral state. Assuming that the dimensions of the substrate binding cavity do not change during the excited state formation, a His22 residue within hydrogen bonding distance to the 6-(p-hydroxy)-phenyl group of the excited coelenteramide is a likely candidate for accepting the phenol proton to produce an ion-pair excited state, in support of recent suggestions for the bioluminescence emitting state. The proton transfer could be impeded by removal of the Trp92 H-bond, resulting in strong enhancement of a 405 nm band giving the violet color of bioluminescence. Comparative analysis of 3D structures of the wild-type (WT) and W92F obelins reveals that there are structural displacements of certain key Ca2+-ligating residues in the loops of the two C-terminal EF hands as well as clear differences in hydrogen bond networks in W92F. For instance, the hydrogen bond between the side-chain oxygen atom of Asp 169 and the main-chain nitrogen of Arg112 binds together the incoming alpha-helix of loop III and the exiting cc-helix of loop IV in WT, providing probably concerted changes in these EF hands on calcium binding. But this linkage is not found in W92F obelin. These differences apparently do not change the overall affinity to calcium of W92F obelin but may account for the kinetic differences between the WT and mutant obelins. From analysis of the hydrogen bond network in the coelenterazine binding cavity, it is proposed that the trigger for bioluminescence reaction in these Ca2+-regulated photoproteins may be a shift of the hydrogen bond donor-acceptor separations around the coelenterazine-2-hydroperoxy substrate, initiated by small spatial adjustment of the exiting a-helix of loop IV.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Anishchenko O.V., Sushchnik N.N., Kalacheva G.S., Gribovskaya I.V., Ageev A.V.
Заглавие : Influence of anthropogenic pollution on content of essential polyunsaturated fatty acids in links of food chain of river ecosystem
Место публикации : Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - С. 376-385. - ISSN 19954255 (ISSN) , DOI 10.1134/S1995425512040051
Ключевые слова (''Своб.индексиров.''): gammarus--grayling--heavy metals--polyunsaturated fatty acids--amphipod--anthropogenic effect--anthropogenic source--fatty acid--food chain--heavy metal--littoral environment--oil pollution--phenol--river system--salmonid--sampling--krasnoyarsk [russian federation]--russian federation--yenisei river--gammarus
Аннотация: In the course of monthly sampling in 2008-2010, two regions of the littoral of the Yenisei river were compared. One of these regions (conventionally pure) was situated upstream of Krasnoyarsk, while the other (conventionally polluted) was downstream of Krasnoyarsk. The concentrations of heavy metals, oil products, phenols, biogenic elements and essential polyunsaturated fatty acids (PUFAs) in various components of the river ecosystem were determined. It was discovered that the anthropogenic pollution causes a decrease in the resources of essential PUFA in the biomass of the upper links of the food chain of the river ecosystem. В© 2012 Pleiades Publishing, Ltd.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Kalachova G.S., Shchur L.A.
Заглавие : The effect of algal blooms on the disappearance of phenol in a small forest pond
Место публикации : Water Research. - 1998. - Vol. 32, Is. 9. - С. 2769-2775. - ISSN 00431354 (ISSN) , DOI 10.1016/S0043-1354(98)00009-8
Ключевые слова (''Своб.индексиров.''): algal blooms--phenol--seasonal dynamics of biodegradation--self-purification--algae--biodegradation--ecosystems--phenols--purification--reaction kinetics--reservoirs (water)--surface waters--experimental microecosystems--forest pond waters--green algae volvox aureus--inorganic nutrients--krasnoyarsk reservoir--water pollution--lake water--phenol--article--ecosystem--forest--green alga--priority journal--russian federation--water pollutant--water temperature
Аннотация: Using experimental microecosystems the kinetics of phenol disappearance in small forest pond waters (Siberia, Russia) in the summer of 1995-96 were investigated. Despite of high variability of components of the ecosystem (plankton biomass and species composition) and two pronounced 'blooms' of green algae Volvox aureus the same kinetics of the disappearance took place over the investigated period. Half-lives of the pollutant depended on water temperature only. A comparison of the self-purification of the pond with that of the Krasnoyarsk reservoir, 'blooming' with blue-greens was carried out. Half-lives in the pond were significantly lower than that in the reservoir. During the periods of 'blooms' of the green algae in the pond the concentrations of inorganic nutrients were comparatively high and the phenol-degrading bacteria likely were not limited by these nutrients, in contrast to the periods of 'bloom' of the blue-green algae in the reservoir.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stom D.I., Geel T.A., Balayan A.E., Shachova G.I., Kuznetsov A.M., Medvedeva S.E.
Заглавие : Bioluminescent method in studying the complex effect of sewage components
Место публикации : Archives of Environmental Contamination and Toxicology. - 1992. - Vol. 22, Is. 2. - С. 203-208. - ISSN 00904341 (ISSN)
Ключевые слова (''Своб.индексиров.''): heavy metal--phenol derivative--quinone derivative--article--bacterium--bioluminescence--cell membrane--nonhuman--priority journal--sludge--ultrastructure--benzoquinones--catechin--hydroquinones--luminescent measurements--metals--phenol--phenols--photobacterium--sewage--vibrio--water pollutants, chemical--bacteria (microorganisms)
Аннотация: The inhibition of bacterial luminescence has been used in testing industrial enterprises sewage. The toxicity of the sewage is less than the total toxicity of separate components due to neutralization of quinone products of polyphenol oxidation in the reactions with the other phenol components of sewage. Toxicity increase is due to their influence on the cell membrane. Studies of cell ultrastructure confirm this fact. The studied mechanism of the complex effect allowed a more accurate forecast of the ecological situation during the discharge of phenol compounds and metals. It also showed the necessity of taking into account the complex effect of sewage components on contaminant discharge into water reservoirs.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Kalacheva G.S., Shchur L.A.
Заглавие : Composition of free fatty acids of the surface film of water and kinetic of self-purification from phenol in forest pond at ''bloom'' of Volvox aureus
Колич.характеристики :4 с
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1996. - Vol. 349, Is. 6. - С. 840-843. - ISSN 0869-5652
Примечания : Cited References: 5
Предметные рубрики: KRASNOYARSK RESERVOIR
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