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Antioxidant activity of humic substances via bioluminescent monitoring in vitro [Text] / A. S. Tarasova, D. I. Stom, N. S. Kudryasheva // Environ. Monit. Assess. - 2015. - Vol. 187, Is. 3. - Ст. 89, DOI 10.1007/s10661-015-4304-1. - Cited References:51. - This work was supported by the Russian Foundation for Basic Research, Grant No. 15-03-06786a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1. . - ISSN 0167-6369. - ISSN 1573-2959

РУБ Environmental Sciences
Рубрики:
DETOXIFICATION PROCESSES
   TOXICITY
   BIOASSAYS
   BACTERIA
   ASSAY
Кл.слова (ненормированные):
Antioxidant activity -- Oxidative toxicity -- General toxicity -- Humic -- substances -- Bioassay -- Bioluminescence
Аннотация: This work considers antioxidant properties of natural detoxifying agents-humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature-complex salt K-3[Fe(CN)(6)] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P) H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters-bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients D-GT and D-OxT, respectively. Dependencies of D-GT and D-OxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were >20-min incubation time and 0.5x10(-4) to 2x10(-4) M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Irkutsk State Univ, Irkutsk 664003, Russia.
ИБФ СО РАН

Доп.точки доступа:
Tarasova, A.S.; Stom, D.I.; Kudryasheva, N.S.; Russian Foundation for Basic Research [15-03-06786a]; Russian Academy of Sciences [VI 57.1.1]

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Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring [Text] / N. S. Kudryasheva, A. S. Tarasova // Environ. Sci. Pollut. Res. - 2015. - Vol. 22, Is. 1. - P155-167, DOI 10.1007/s11356-014-3459-6. - Cited References:120. - The work was supported by the Russian Foundation for Basic Research,Grant No. 13-04-98072-sibir-a. Part of the work (analysis ofdetoxification of radioactive solutions) was supported by the RussianScience Foundation, Grant No. 14-14-00076. . - ISSN 0944-1344. - ISSN 1614-7499

РУБ Environmental Sciences
Рубрики:
PHOTOBACTERIUM-LEIOGNATHI LUCIFERASE
DISSOLVED ORGANIC-MATTER
Кл.слова (ненормированные):
Detoxification mechanisms -- Humic substances -- Pollutants -- Bioassays -- Bioluminescence
Аннотация: The paper considers mechanisms of detoxification of pollutant solutions by water-soluble humic substances (HSs), natural detoxifying agents. The problems and perspectives of bioassay application for toxicity monitoring of complex solutions are discussed from ecological point of view. Bioluminescence assays based on marine bacteria and their enzymes are of special attention here; they were shown to be convenient tools to study the detoxifying effects on cellular and biochemical levels. The advantages of bioluminescent enzymatic assay for monitoring both integral and oxidative toxicities in complex solutions of model pollutants and HS were demonstrated. The efficiencies of detoxification of the solutions of organic oxidizers and salts of metals (including radioactive ones) by HS were analyzed. The dependencies of detoxification efficiency on time of exposure to HS and HS concentrations were demonstrated. Antioxidant properties of HS were considered in detail. The detoxifying effects of HS were shown to be complex and regarded as 'external' (binding and redox processes in solutions outside the organisms) and/or 'internal' organismal processes. The paper demonstrates that the HS can stimulate a protective response of bacterial cells as a result of (1) changes of rates of biochemical reactions and (2) stabilization of mucous layers outside the cell walls. Acceleration of auto-oxidation of NADH, endogenous reducer, by HS was suggested as a reason for toxicity increase in the presence of HS due to abatement of reduction ability of intracellular media.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
RAS, Inst Biophys, SB, Krasnoyarsk 660036, Russia.
ИБФ СО РАН

Доп.точки доступа:
Kudryasheva, N.S.; Tarasova, A.S.; Russian Foundation for Basic Research [13-04-98072-sibir-a]; RussianScience Foundation [14-14-00076]

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Detonation Nanodiamonds as a New Tool for Phenol Detection in Aqueous Medium / N. Ronzhin, A. Puzyr, V. Bondar // J. Nanosci. Nanotechnol. - 2018. - Vol. 18, Is. 8. - P5448-5453, DOI 10.1166/jnn.2018.15382. - Cited References:27. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (Project No. 0356-2016-0709) and Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund to the research (Project No. 16-43-243027). . - ISSN 1533-4880. - ISSN 1533-4899

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
ENVIRONMENTAL-POLLUTANTS
   DRUG-DELIVERY
   PARTICLES
   CARBON
Кл.слова (ненормированные):
Detonation Nanodiamonds -- Catalytic Activity -- Metal Ions -- Azo Coupling -- Reaction -- Phenol Detection
Аннотация: This paper demonstrates the effectiveness of using detonation nanodiamonds (DNDs) for detecting phenol in aqueous medium. The study has shown that the catalytic effect of DNDs in the oxidative azo coupling reaction (phenol-4-aminoantipyrine-hydrogen peroxide) is produced by trace amounts of iron and copper ions adsorbed on the surface of nanoparticles. The effectiveness of DNDs as a catalyst is determined by the amounts of these adsorbates and can be enhanced by a factor of two by additional adsorption of these ions onto the nanoparticles. A rise in the temperature of the ONO-catalyzed azo coupling reaction leads to a considerable (4.5-fold) increase in the reaction product yield. DNDs used to detect phenol in aqueous medium enable a linear increase in the yield of the product of the azo coupling reaction at concentrations of the analyte of between 0.05 and 10 mu g/mlThe study demonstrates that DNDs can be reused to detect phenol in water samples.

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Держатели документа:
RAS, SB, Krasnoyarsk Sci Ctr, Inst Biophys,Fed Res Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ronzhin, Nikita; Puzyr, Alexey; Bondar, Vladimir; Russian Academy of Sciences [0356-2016-0709]; Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology [16-43-243027]

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A small closed ecosystem with an estimated portion of human metabolism / A. A. Tikhomirov [et al.] // Life Sci. Space Res. - 2018. - Vol. 19. - P63-67, DOI 10.1016/j.lssr.2018.10.001. - Cited References:11. - The study was supported by the Russian Science Foundation (Project No. 14-14-00599.) and carried out in the IBP SB RAS at FRC KRC SB RAS. . - ISSN 2214-5524. - ISSN 2214-5532

РУБ Astronomy & Astrophysics + Biology + Multidisciplinary Sciences

Кл.слова (ненормированные):
Closed ecosystem -- Physicochemical waste processing -- Biochemical waste -- processing -- Cycling process
Аннотация: The study describes a small closed ecosystem used to test technologies to be further employed in full-scale manned closed ecosystems. The experimental ecosystem is designed to use a certain portion of human metabolism, which is included in the gas, water, and organic waste loops of the system. In this experimental ecosystem, gas and water loops are fully closed, and the model enables processing of human waste and plant inedible biomass. A physicochemical method is used to remove pollutants from the air in the system. A human takes part in the gas exchange of the system through its respiration loop. This experimental ecosystem can be used for testing and improving new technologies to be further used in the future space stations.

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Scopus
Держатели документа:
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Tikhomirov, Alexander A.; Ushakova, Sofya A.; Velichko, Vladimir V.; Trifonov, Sergey, V; Tikhomirova, Natalia A.; Kalacheva, Galina S.; Russian Science Foundation [14-14-00599]

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Analytical Enzymatic Reactions in Microfluidic Chips / K. A. Lukyanenko [et al.] // Appl. Biochem. Microbiol. - 2017. - Vol. 53, Is. 7. - P775-780, DOI 10.1134/S0003683817070043. - Cited References:15. - The study was supported by a grant from the Russian Science Foundation (project No. 15-19-10041). . - ISSN 0003-6838. - ISSN 1573-8183

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
BIOAVAILABLE HEAVY-METALS
   DEVICES
   POINT
   LAB
Кл.слова (ненормированные):
bioluminescence -- luciferase -- microfluidics -- microfluidic chip -- enzymatic -- bioassay
Аннотация: A number of approaches have been proposed and tested to transfer enzymatic reactions into the functional elements of microfluidic chips on the example of the bienzyme bioluminescent reaction involving NAD(P)H:FMN-oxidoreductase and luciferase. Measurement of the catalytic activity of these enzymes (under the influence of pollutants) is the basis of enzymatic bioassay of various liquids. It was found that all of the components of the reaction must be placed in the same cell of the chip to improve the reproducibility of the measurements. The use of starch gel as a carrier for immobilization and gelatin as a scaffold in the reactor of the chip enables the preservation of enzyme activity in the course of sealing the chip at room temperature. It is shown that the components of the reaction should be vigorously stirred in a microfluidic chip reactor to improve the efficiency of the analysis. As a result of the studies, a prototype of microfluidic chip based on the enzymatic bioluminescent reaction is proposed. It is characterized by a detection limit of copper sulfate of 3 mu M that corresponds to the sensitivity of traditional lux-biosensors based on living cells. The analysis time is reduced to 1 min, and the analysis can be performed by individuals without special laboratory skills.

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Scopus
Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
St Petersburg Inst Fine Mech & Opt, St Petersburg 197101, Russia.
Inst Analyt Instrumentat, St Petersburg 198095, Russia.

Доп.точки доступа:
Lukyanenko, K. A.; Denisov, I. A.; Yakimov, A. S.; Esimbekova, E. N.; Belousov, K. I.; Bukatin, A. S.; Kukhtevich, I. V.; Sorokin, V. V.; Evstrapov, A. A.; Belobrov, P. I.; Russian Science Foundation [15-19-10041]

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Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River [Text] / M. Y. Medvedeva, A. Y. Bolsunovsky, T. A. Zotina // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 4. - P422-432, DOI 10.1134/S1995425514040088. - Cited References: 19. - The authors thank their colleagues from the Radioecology laboratory and Analytical laboratory for gamma spectrometric and chemical analyses. The authors express their appreciation to O.V. Kvitko (Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk) for her valuable methodical consultations. This research is partially funded by both a RFFI-r_Siberia-a, grant no. 13-04-98004 from the Russian Foundation for Basic Research and project no. 30.5 of the Biological Diversity Program of the Presidium of the Russian Academy of Sciences. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
ALLIUM-TEST
GENOTOXICITY
TOXICITY
Кл.слова (ненормированные):
Elodea canadensis -- bottom sediments -- chromosome abnormalities -- anthropogenic radionuclides -- heavy metals -- genotoxicity
Аннотация: Chromosome abnormalities in ana-telophase cells of apical root meristem of aquatic plant Elodea. canadensis (elodea), sampled in 2011-2012 in the Yenisei River at a site with background level of contamination and at several sites on the stretch contaminated with artificial radionuclides, and with chemical pollutants from municipal and industrial discharges of the Krasnoyarsk city. Lowest rate (5.2%) of cells with chromosome abnormalities was registered at sampling site with background level of contamination upstream of the Krasnoyarsk, highest rate of cells with abnormalities (39.7%)-in roots of elodea sampled in bottom sediments with highest concentration of Cs-137. Sum of rates of cells with abnormalities and rates of cells with all types of abnormalities positively correlated with total concentration of artificial and natural radionuclides, with concentration of artificial radionuclides and Cs-137 in bottom sediments of the Yenisei River (r (2) = 0.91-0.96, p < 0.0005 for sum of rates of cells with abnormalities; r (2) = 0.58-0.92, p < 0.05 for all types of abnormalities).

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Держатели документа:
[Medvedeva, M. Yu
Bolsunovsky, A. Ya
Zotina, T. A.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, M.Y.; Bolsunovsky, A.Y.; Zotina, T.A.; RFFI-r_Siberia-a from the Russian Foundation for Basic Research [13-04-98004]; Biological Diversity Program of the Presidium of the Russian Academy of Sciences [30.5]

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(19)F NMR study on the biodegradation of fluorophenols by various Rhodococcus species [Text] / V. S. Bondar [et al.] // Biodegradation. - 1998. - Vol. 9: NMR in Environmental Sciences Symposium (OCT 24, 1997, EDE, NETHERLANDS), Is. 6. - P475-486, DOI 10.1023/A:1008391906885. - Cited References: 37 . - ISSN 0923-9820

РУБ Biotechnology & Applied Microbiology
Рубрики:
NUCLEAR-MAGNETIC-RESONANCE
   PSEUDOMONAS-PUTIDA MT-2
   PHENOL HYDROXYLASE
   BACTERIAL-DEGRADATION
   3-OXOADIPATE PATHWAY
   FLUOROBENZOIC ACID
   CONVERSION
   METABOLISM
   CYCLOISOMERASES
   2-CHLORO-CIS,CIS-MUCONATE
Кл.слова (ненормированные):
biodegradation -- fluorophenols -- (19)F NMR -- oxidative defluorination -- Rhodococcus species
Аннотация: Of all NMR observable isotopes (19)F is the one perhaps most convenient for studies on biodegradation of environmental pollutants. The reasons underlying this potential of (19)F NMR are discussed and illustrated on the basis of a study on the biodegradation of fluorophenols by four Rhodococcus strains. The results indicate marked differences between the biodegradation pathways of fluorophenols among the various Rhodococcus species. This holds not only for the level and nature of the fluorinated biodegradation pathway intermediates that accumulate, but also for the regioselectivity of the initial hydroxylation step. Several of the Rhodococcus species contain a phenol hydroxylase that catalyses the oxidative defluorination of ortho-fluorinated di- and trifluorophenols. Furthermore, it is illustrated how the (19)F NMR technique can be used as a tool in the process of identification of an accumulated unknown metabolite, in this case most likely 5-fluoromaleylacetate. Altogether, the (19)F NMR technique proved valid to obtain detailed information on the microbial biodegradation pathways of fluorinated organics, but also to provide information on the specificity of enzymes generally considered unstable and, for this reason, not much studied so far.

Держатели документа:
Agr Univ Wageningen, Dept Biomol Sci, Biochem Lab, NL-6703 HA Wageningen, Netherlands
Russian Acad Sci, Inst Biochem & Physiol Microorganisms, Pushchino 142292, Russia
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondar, V.S.; Boersma, M.G.; Golovlev, E.L.; Vervoort, J...; Van Berkel, WJH; Finkelstein, Z.I.; Solyanikova, I.P.; Golovleva, L.A.; Rietjens, IMCM

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A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants [Text] / A. B. Sarangova, L. A. Somova ; ed. RM Wheeler [et al.] // LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P. 2049-2052, DOI 10.1016/S0273-1177(97)00940-X. - Cited References: 4 . - ISBN 0273-1177. - ISBN 0-08-043307-3

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. (C) 1997 COSPAR. Published by Elsevier Science Ltd.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Wheeler, RM \ed.\; Garland, JL \ed.\; Tibbitts, TW \ed.\; Nielsen, SS \ed.\

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Bioluminescent water quality monitoring of salt lake Shira [Text] / V. A. Kratasyuk, E. V. Vetrova, N. S. Kudryasheva // Luminescence. - 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P. 193-195, DOI 10.1002/(SICI)1522-7243(199907/08)14:4193::AID-BIO5283.3.CO;2-J. - Cited References: 9 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Кл.слова (ненормированные):
bioluminescence -- biotest -- ecological monitoring -- salt lake
Аннотация: The coupled bioluminescent enzyme system luciferase-NADH:FMN-oxidoreductase was used as a biotest in ecological monitoring of the health resort salt lake Shira (South Siberia, Russia). The technique was adapted to saltwater conditions. Bioluminescence kinetic parameters sensitive to pollutants were determined. Conditions for the use of bacterial bioluminescence biotests in salty environmental media were established. Copyright (C) 1999 John Wiley & Sons, Ltd.

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Держатели документа:
Russian Acad Sci, Inst Biophys, SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Vetrova, E.V.; Kudryasheva, N.S.

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10.

Development of bioluminescent bioindicators for analysis of environmental pollution [Text] / N. . Kudryasheva [et al.] // Field Anal. Chem. Technol. - 1998. - Vol. 2, Is. 5. - P. 277-280, DOI 10.1002/(SICI)1520-6521(1998)2:5277::AID-FACT43.0.CO;2-P. - Cited References: 17 . - ISSN 1086-900X

РУБ Chemistry, Analytical + Environmental Sciences + Instruments & Instrumentation

Кл.слова (ненормированные):
biotest -- bioluminescence -- enzymes -- environmental monitoring
Аннотация: The influence of several suites of pollutants (metallic salts, quinones, and phenols) on bacterial bioluminescence in vivo and in vitro (five test systems) was investigated. The sensitivity of bioluminescence to the different pollutants was evaluated, and inhibition constants were measured. The data obtained were shown to correlate with the physical and chemical characteristics of the substances and the structure of the bioluminescent systems. It has been found that three bioluminescent tests (water-soluble enzyme systems, immobilized enzyme systems, and bioluminescent bacteria) show higher sensitivity to pollutants and cover all types of widespread contamination. These tests were chosen as a set of bioluminescent assays for the detection of pollutants. (C) 1998 John Wiley & Sons, Inc.

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Держатели документа:
RAS, Inst Biophys, SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N...; Kratasyuk, V...; Esimbekova, E...; Vetrova, E...; Nemtseva, E...; Kudinova, I...

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11.

Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils / E. M. Kolosova, O. S. Sutormin, L. V. Stepanova [et al.] // Environ. Technol. Innov. - 2021. - Vol. 24. - Ст. 101842, DOI 10.1016/j.eti.2021.101842 . - ISSN 2352-1864
Кл.слова (ненормированные):
Bioassay -- Bioluminescence -- Industrial contamination -- Soil pollution -- Urbostratozems -- Arsenic -- Chemical analysis -- Enzyme activity -- Enzyme inhibition -- Fluorine compounds -- Soil surveys -- Soil testing -- Soils -- Toxicity -- Arsenic concentration -- Chemical and biologicals -- Comprehensive information -- Contaminated soils -- Environmental assessment methods -- Enzymatic bioassays -- Luminescent bacteria -- Sample preparation -- Soil pollution
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. © 2021 Elsevier B.V.

Scopus
Держатели документа:
Department of Biophysics, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Krasnoyarsk Agricultural Research Institute, Federal Research Center ‘Krasnoyarsk Science Center Siberian Branch of the Russian Academy of Sciences’, Krasnoyarsk, 660036, Russian Federation
Department of Aquatic and Terrestrial Ecosystems, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Photobiology Laboratory, Institute of Biophysics, Federal Research Center ‘Krasnoyarsk Science Center Siberian Branch of the Russian Academy of Sciences’, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Kolosova, E. M.; Sutormin, O. S.; Stepanova, L. V.; Shpedt, A. A.; Rimatskaya, N. V.; Sukovataya, I. E.; Kratasyuk, V. A.

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12.

Direct and Indirect Detoxification Effects of Humic Substances / L. Bondareva, N. Kudryasheva // Agronomy-Basel. - 2021. - Vol. 11, Is. 2. - Ст. 198, DOI 10.3390/agronomy11020198. - Cited References:79. - This review was prepared with the partial financial support of the Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Russian Federation) 2020-2025. . - ISSN 2073-4395

РУБ Agronomy + Plant Sciences

Кл.слова (ненормированные):
humic substances -- detoxification -- luminous bacteria -- adaptive response
Аннотация: The review summarizes studies on the detoxification effects of water-soluble humic substances (HS), which are products of the natural transformation of organic substances in soils and bottom sediments that serve as natural detoxifying agents in water solutions. The detoxifying effects of HS on microorganisms are quite complex: HS neutralize free pollutants (indirect bioeffects) and also stimulate the protective response of organisms (direct bioeffects). Prospects and potential problems of bioluminescent bacteria-based assay to monitor toxicity of solutions in the presence of HS are discussed. The main criterion for the bioassay application is versatility and ease of use. The detoxification efficiency of HS in different pollutant solutions was evaluated, and the detoxification mechanisms are discussed. Particular attention was paid to the direct and complex direct + indirect effects of HS. The review focuses on the protective function of HS in solutions of radionuclides and salts of stable metals, with special consideration of the antioxidant properties of HS.

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Держатели документа:
Fed Sci Ctr Hyg, Moscow 141014, Russia.
Russian Acad Sci, Inst Biophys, Krasnoyarsk Sci Ctr, Fed Res Ctr,Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Bondareva, Lydia; Kudryasheva, Nadezhda; Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Russian Federation) 2020-2025

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13.

Hydrochemical Indicators of Water Quality in the Norilsk-Pyasino Lake-River System after a Diesel Fuel Spill at Norilsk Heat and Power Plant 3 in 2020 / D. M. Bezmaternykh, A. V. Puzanov, A. V. Kotovshchikov [et al.] // Contemp. Probl. Ecol. - 2021. - Vol. 14, Is. 4. - P323-334, DOI 10.1134/S1995425521040028. - Cited References:22 . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology

Кл.слова (ненормированные):
hydrochemistry -- water quality -- Pyasino River -- Lake Pyasino -- Norilsk -- diesel fuel
Аннотация: The results of a hydrochemical analysis of the consequences of an accidental fuel release in the Norilsk-Pyasino water system are presented. The pollution of watercourses in the catchment of Lake Pyasino (a nameless (Nadezhdinsky) creek, the Daldykan River, and the Ambarnaya River) with oil products, phenols, easily oxidizable and hard to oxidize organic matter (COD, PO, and BOD5), suspended solids, inorganic salts, and heavy metals at concentrations exceeding the background levels and MPC for fishery water bodies, as well as a temperature rise in waters of the nameless creek near Norilsk Heat and Power Plant 3 (CHPP-3), have been revealed. The contamination of the surface water decreases downstream in ascending order: nameless creek-Daldykan River-Ambarnaya River. The occurrence of oil products, phenols, and organic substances in the surface waters 2 months after the fuel spill is obviously due to their diffusion from the river bottom sediments, which accumulated a considerable quantity of heavy fractions of diesel fuel after the accident. Increased concentrations of Ca, Cu, Zn, Mn, Co, and Ni in the waters of the studied tributaries of Lake Pyasino are not directly related to the accident; they result from the general technogenic pollution of the territory and the increased geochemical background for these elements. Water contamination with oil products and phenols in the studied areas of Lake Pyasino (its central and northern parts) and the Pyasino River has not been detected. However, Pb concentrations exceed the MPC and Cd is recorded in the water, which is probably due to pollutants that accumulated in previous years.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Water & Environm Problems, Barnaul 656038, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Bezmaternykh, D. M.; Puzanov, A. V.; Kotovshchikov, A. V.; Drobotov, A. V.; Tolomeev, A. P.

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14.

РУБ Biotechnology & Applied Microbiology + Engineering, Environmental
Рубрики:
FLUORIDE
   BIOASSAYS
   POLLUTION
   METALS
   WATER
Кл.слова (ненормированные):
Urbostratozems -- Soil pollution -- Industrial contamination -- Bioassay -- Bioluminescence
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. (C) 2021 Elsevier B.V. All rights reserved.

WOS
Держатели документа:
Siberian Fed Univ, Dept Biophys, 79 Svobodny St, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Krasnoyarsk Agr Res Inst, Fed Res Ctr Krasnoyarsk Sci Ctr Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Dept Aquat & Terr Ecosyst, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Photobiol Lab, Inst Biophys, Fed Res Ctr `Krasnoyarsk Sci Ctr Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Kolosova, Elizaveta M.; Sutormin, Oleg S.; Stepanova, L. V.; Shpedt, Aleksandr A.; Rimatskaya, N. V.; Sukovataya, Irina E.; Kratasyuk, Valentina A.; Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia; Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [18-47-240005]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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15.

Hydrochemical Indicators of Water Quality in the Norilsk–Pyasino Lake–River System after a Diesel Fuel Spill at Norilsk Heat and Power Plant 3 in 2020 / D. M. Bezmaternykh, A. V. Puzanov, A. V. Kotovshchikov [et al.] // Contemp. Probl. Ecol. - 2021. - Vol. 14, Is. 4. - P323-334, DOI 10.1134/S1995425521040028 . - ISSN 1995-4255
Кл.слова (ненормированные):
diesel fuel -- hydrochemistry -- Lake Pyasino -- Norilsk -- Pyasino River -- water quality
Аннотация: Abstract: The results of a hydrochemical analysis of the consequences of an accidental fuel release in the Norilsk–Pyasino water system are presented. The pollution of watercourses in the catchment of Lake Pyasino (a nameless (Nadezhdinsky) creek, the Daldykan River, and the Ambarnaya River) with oil products, phenols, easily oxidizable and hard to oxidize organic matter (COD, PO, and BOD5), suspended solids, inorganic salts, and heavy metals at concentrations exceeding the background levels and MPC for fishery water bodies, as well as a temperature rise in waters of the nameless creek near Norilsk Heat and Power Plant 3 (CHPP-3), have been revealed. The contamination of the surface water decreases downstream in ascending order: nameless creek–Daldykan River–Ambarnaya River. The occurrence of oil products, phenols, and organic substances in the surface waters 2 months after the fuel spill is obviously due to their diffusion from the river bottom sediments, which accumulated a considerable quantity of heavy fractions of diesel fuel after the accident. Increased concentrations of Ca, Cu, Zn, Mn, Co, and Ni in the waters of the studied tributaries of Lake Pyasino are not directly related to the accident; they result from the general technogenic pollution of the territory and the increased geochemical background for these elements. Water contamination with oil products and phenols in the studied areas of Lake Pyasino (its central and northern parts) and the Pyasino River has not been detected. However, Pb concentrations exceed the MPC and Cd is recorded in the water, which is probably due to pollutants that accumulated in previous years. © 2021, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute for Water and Environmental Problems, Siberian Branch, Russian Academy of Sciences, Barnaul, 656038, Russian Federation
Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Bezmaternykh, D. M.; Puzanov, A. V.; Kotovshchikov, A. V.; Drobotov, A. V.; Tolomeev, A. P.

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16.

Assessment of the Quality of Bottom Sediments in the Middle Reaches of the Yenisei River by Allium test / T. A. Zotina [et al.] // Contemp. Probl. Ecol. - 2019. - Vol. 12, Is. 3. - P265-274, DOI 10.1134/S1995425519030120. - Cited References:35 . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
PLANT ELODEA-CANADENSIS
ANTHROPOGENIC CONTAMINATION
Кл.слова (ненормированные):
ana-telophase -- genotoxicity -- root length -- artificial sediments -- mitotic -- index -- toxicity
Аннотация: The harmful potential of bulk bottom sediments of the Yenisei River has been assessed by the Allium test. Sediment samples have been taken in ten sites on a plot of about 100 km in length in the middle reaches of the Yenisei River affected by urban activity of the city of Krasnoyarsk and industrial plants. The samples of sediments differ considerably in the content of potentially toxic substances (heavy metals, artificial radionuclides, and organic pollutants) and nutrients and in physical properties. We use the mean length of onion root as an indicator of general toxicity, the mitotic index as an indicator of cytotoxicity, and the percentage of anatelophase cells in the apical root meristem with abnormal chromosomes as an indicator of genotoxicity. Both the stimulation and inhibition of indicator endpoints are revealed in sediment samples of the Yenisei River relative to the control (artificial sediments). An increased genotoxicity is recorded in the sample of sediments contaminated by a mixture of organic and chemical toxicants. A significant positive correlation between the percentage of abnormal cells and the content of copper and petrochemicals in the samples is revealed. No extremely strong reactions of onion endpoints to the quality of sediment samples are revealed, which is probably related to relatively low contamination of the sediments studied. This is in agreement with the data of chemical analyses. Based on the testing results, we can conclude that the endpoints of the Allium test are sensitive enough to the quality of bottom sediments of the Yenisei River and can be used for their biotesting.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Fed Res Ctr, Inst Biophys,Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zotina, T. A.; Trofimova, E. A.; Alexandrova, Yu. V.; Anishchenko, O. V.

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17.

Converting gaseous pollutants toxic to plants and humans into environmentally friendly compounds in artificial ecosystems / A. A. Tikhomirov [et al.] // IOP Conference Series: Materials Science and Engineering : Institute of Physics Publishing, 2018. - Vol. 450: 9th International Multidisciplinary Scientific and Research Conference on Modern Issues in Science and Technology Workshop in Advanced Technologies in Aerospace, Mechanical and Automation Engineering, MISTAerospace 2018 (20 October 2018 through 28 October 2018, ) Conference code: 143027, Is. 6, DOI 10.1088/1757-899X/450/6/062004
Кл.слова (ненормированные):
Ecosystems -- Environmental management -- Fog -- Artificial ecosystems -- Experimental conditions -- Gaseous pollutants -- Human waste -- Liquid products -- Plant growth -- Toxic effect -- Wheat plants -- Pollution
Аннотация: The present study describes detection of potential gaseous pollutants that can produce a toxic effect on plants and humans in the system with wheat plants cultivated on solutions containing liquid products of mineralization of human waste and fish waste. These gaseous pollutants do not inhibit plant growth and development under the experimental conditions, but they may accumulate in closed ecosystems functioning for extended periods of time. Ways to convert gaseous pollutants into environmentally friendly compounds have been proposed. © 2018 Institute of Physics Publishing. All rights reserved.

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WOS
Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Akademgorodok, 660036, Russian Federation

Доп.точки доступа:
Tikhomirov, A. A.; Ushakova, S. A.; Tikhomirova, N. A.; Trifonov, S. V.; Kalacheva, G. S.

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18.

Pine Stands as Bioindicators: Justification for Air Toxicity Monitoring in an Industrial Metropolis / G. Polyakova, N. Pashenova, V. Senashova [et al.] // Environments. - 2020. - Vol. 7, Is. 4. - Ст. 28, DOI 10.3390/environments7040028. - Cited References:35. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, projects 0356-2018-0742 and 0356-2017-0017, and by RFBR grant 15-04-06575, RFBR-Krasnoyarsk Regional Foundation N 18-44-240004, grant 18-44-242002. . - ISSN 2076-3298

РУБ Environmental Sciences
Рубрики:
HORMESIS
Кл.слова (ненормированные):
environmental monitoring -- pollutants -- toxicity -- creeping fire -- high-grade pine stands -- bioindicators
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middle-aged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich's model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye's adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown.

WOS
Держатели документа:
FRC KSC SB RAS, Sukachev Inst Forest SB RAS, Krasnoyarsk 660036, Russia.
Nonstate Educ Inst Higher Profess Educ Siberian I, Krasnoyarsk 660069, Russia.
FRC KSC SB RAS, Photobiol Lab, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Polyakova, Galina; Pashenova, Natalia; Senashova, Vera; Podolyak, Natalia; Kudryasheva, Nadezhda; Russian Academy of SciencesRussian Academy of Sciences [0356-2018-0742, 0356-2017-0017]; RFBRRussian Foundation for Basic Research (RFBR) [15-04-06575]; RFBR-Krasnoyarsk Regional Foundation [18-44-240004, 18-44-242002]

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19.

Resistance of Dormant Eggs of Cladocera to Anthropogenic Pollutants / E. Zadereev, T. S. Lopatina, N. Oskina ; ed.: V. R. Alekseev, B. . PinelAlloul // Monogr. Biol. : SPRINGER INTERNATIONAL PUBLISHING AG, 2019. - Vol. 92. - P121-135. - (Monographiae Biologicae), DOI 10.1007/978-3-030-21213-1_7. - Cited References:59. - The research was partially supported by the Russian Foundation for Basic Research (Project No. 15-04-05199) and the Krasnoyarsk Regional Science Foundation (Project No. 16-44-243041). We are grateful to Helen Krasova for linguistic improvements. . -

РУБ Marine & Freshwater Biology
Рубрики:
FRESH-WATER CLADOCERAN
RESTING EGGS
IONIZING-RADIATION
Кл.слова (ненормированные):
Resting eggs -- Resistance -- Heavy metals -- Ionizing radiation -- Toxicity
Аннотация: Many Cladocera species under unfavourable conditions produce resting eggs that can survive for years in deep diapause. Resting eggs form egg banks at the bottom sediments, which serve as a source of genetic diversity and replenish the population after periods of decline. Despite the obvious importance of resting eggs for the ecosystem functioning, studies assessing the sensitivity of resting eggs to different toxicants are scarce. We reviewed published data on the sensitivity of resting eggs to the effect of heavy metals, organic pollutants and ionizing radiation. Analysis shows that the effects of toxicants of different types on resting eggs will have different environmental consequences. Egg banks may suffer from prolonged contact of dormant eggs with heavy metals. However, the ecological relevance of these effects is low, since the effective concentrations of toxicants must be very high. In addition, the effect of heavy metals on resting eggs is not transmitted to hatchlings from exposed eggs. Taking into account high toxicity of heavy metals to active animals, we assume that the toxic effect of heavy metals is critical for active zooplankters and relatively safe for resting stages. Accumulation of artificial radionuclides in bottom sediments can have a significant impact on aquatic ecosystems through chronic effects both on survival of resting eggs and on the life cycle parameters of animals hatched from irradiated eggs. Resting eggs during reactivation are more sensitive to the effect of ionizing radiation. Pesticides and complex chemical compounds produce similar toxic effects on both resting eggs and life history parameters of hatchlings from exposed resting eggs. The bottom line is that to predict the effect of contamination of bottom sediments by different pollutants on the ecosystem structure and functioning, it is highly important to investigate the viability of the resting eggs under a wide range of concentrations of various toxicants and different durations of direct contact of eggs with toxicants.

WOS
Держатели документа:
Krasnoyarsk Res Ctr SB RAS, Inst Biophys, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Zadereev, Egor; Lopatina, Tatiana S.; Oskina, Natalia; Alekseev, V.R. \ed.\; PinelAlloul, B... \ed.\; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [15-04-05199]; Krasnoyarsk Regional Science Foundation [16-44-243041]

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20.

Pine stands as bioindicators: Justification for air toxicity monitoring in an industrial metropolis / G. Polyakova, N. Pashenova, V. Senashova [et al.] // Environ. - MDPI. - 2020. - Vol. 7, Is. 4. - Ст. 28, DOI 10.3390/environments7040028 . - ISSN 2076-3298
Кл.слова (ненормированные):
Bioindicators -- Creeping fire -- Environmental monitoring -- High-grade pine stands -- Pollutants -- Toxicity
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middleaged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich‘s model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye‘s adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown. © 2020 by the authors.

Scopus
Держатели документа:
Sukachev Institute of Forest SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Non-State Educational Institution of Higher Professional Education Siberian Institute of Business, Management and Psychology, Krasnoyarsk, 660069, Russian Federation
Photobiology Lab, Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Biophysics Department, Siberian Federal University, Krasnoyarsk, 660049, Russian Federation

Доп.точки доступа:
Polyakova, G.; Pashenova, N.; Senashova, V.; Podolyak, N.; Kudryasheva, N.

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