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Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics / S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180 . - ISSN 0045-6535
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd

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Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation

Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.

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Properties of degradable polyhydroxyalkanoates with different monomer compositions / T. Volova, E. Kiselev, I. Nemtsev [et al.] // Int. J. Biol. Macromol. - 2021. - Vol. 182. - P98-114, DOI 10.1016/j.ijbiomac.2021.04.008 . - ISSN 0141-8130
Кл.слова (ненормированные):
Chemical composition -- Films -- Microstructure -- Physicochemical properties -- Polyhydroxyalkanoates -- Surface properties -- 3 hydroxybutyric acid -- 3 hydroxyhexanoate -- 3 hydroxyvalerate -- 4 hydroxybutyric acid -- monomer -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polymer -- unclassified drug -- Article -- chemical composition -- comparative study -- crystallization -- degradation -- dispersity -- elasticity -- glass transition temperature -- hydrophilicity -- melting point -- molecular weight -- surface property -- synthesis -- thermoregulation -- thermostability
Аннотация: Purpose: To synthesize and investigate polyhydroxyalkanoates (PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390–600 kDa), and increased values of polydispersity (3.2–4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100–120 °C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20–40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB – 3HHx – 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3-hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials. © 2021 Elsevier B.V.

Scopus
Держатели документа:
Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/12 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Volova, T.; Kiselev, E.; Nemtsev, I.; Lukyanenko, А.; Sukovatyi, A.; Kuzmin, A.; Ryltseva, G.; Shishatskaya, E.

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A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals / E. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - P2185-2203, DOI 10.1163/092050610X537138 . - ISSN 0920-5063
Кл.слова (ненормированные):
14C products of polymer resorption -- intravenous administration -- microparticles -- Resorbable polyhydroxybutyrate -- tissues of internal organs -- 3-hydroxybutyric acid -- sup14/supC products of polymer resorption -- Adverse effect -- Drug-delivery systems -- Growth and development -- High molecular weight -- Initial molecular weight -- Initial values -- Internal organs -- Intravenous administration -- Laboratory animals -- Local response -- matrix -- Mean diameter -- Micro-particles -- Polymer degradation -- Polymer microparticles -- Preparation process -- Radioactivity level -- Residual content -- Resorbable -- Sustained-release -- Tail veins -- Wistar rat -- Animals -- Biocompatibility -- Degradation -- Histology -- Molecular weight -- Morphology -- Radiation -- Radioactivity -- Rats -- Tissue -- poly(3 hydroxybutyric acid) -- animal experiment -- animal tissue -- article -- biocompatibility -- concentration process -- controlled study -- degradation -- drug delivery system -- female -- gel permeation chromatography -- heart -- kidney parenchyma -- liver -- lung parenchyma -- molecular weight -- nonhuman -- organ culture -- priority journal -- radioactivity -- rat -- spleen -- Animals -- Animals, Laboratory -- Biocompatible Materials -- Drug Delivery Systems -- Female -- Infusions, Intravenous -- Materials Testing -- Microspheres -- Molecular Weight -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Animalia -- Rattus -- Rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.

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Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.; Goreva, A.; Kalacheva, G.; Volova, T.

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Biocompatibility of polyhydroxybutyrate microspheres: In vitro and in vivo evaluation / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2008. - Vol. 19, Is. 6. - P2493-2502, DOI 10.1007/s10856-007-3345-6 . - ISSN 0957-4530
Кл.слова (ненормированные):
Biocompatibility -- Drug delivery -- Fibroblasts -- Ion implantation -- Microspheres -- Polymer matrix -- Fibroblast cells -- Hydroxybutyric acid -- Intramuscular implantation -- Polyhydroxybutyrate -- Organic polymers -- microsphere -- poly(3 hydroxybutyric acid) -- polymer -- animal cell -- animal experiment -- animal tissue -- article -- biocompatibility -- cell infiltration -- controlled study -- giant cell -- implantation -- in vitro study -- in vivo study -- inflammation -- macrophage -- mouse -- nonhuman -- priority journal -- rat -- 3-Hydroxybutyric Acid -- Animals -- Biocompatible Materials -- Cell Survival -- Inflammation -- Materials Testing -- Mice -- Microspheres -- Necrosis -- NIH 3T3 Cells -- Polymers -- Rats -- Rats, Wistar -- Tetrazolium Salts -- Thiazoles -- Time Factors -- Rattus norvegicus
Аннотация: Microspheres have been prepared from the resorbable linear polyester of ?-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection. В© 2008 Springer Science+Business Media, LLC.

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Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Laboratory of Bacterial Bioluminescence, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svo Bodnyi Av. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Voinova, O.N.; Goreva, A.V.; Mogilnaya, O.A.; Volova, T.G.

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Evaluation of antitumor activity of rubomycin deposited in absorbable polymeric microparticles / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2008. - Vol. 145, Is. 3. - P358-361, DOI 10.1007/s10517-008-0091-9 . - ISSN 0007-4888
Кл.слова (ненормированные):
Absorbable polymers -- Ehrlich's ascitic carcinoma -- Microencapsulation -- Polyhydroxybutyrate -- Rubomycin -- poly(3 hydroxybutyric acid) -- polymer -- rubomycin -- animal cell -- animal experiment -- animal model -- antineoplastic activity -- article -- Bagg albino mouse -- cancer inhibition -- cancer mortality -- cancer survival -- controlled study -- drug delivery system -- Ehrlich ascites tumor -- microencapsulation -- mouse -- nonhuman -- polymerization -- survival rate -- tumor cell -- tumor volume -- Absorption -- Animals -- Antibiotics, Antineoplastic -- Carcinoma, Ehrlich Tumor -- Daunorubicin -- Hydroxybutyrates -- Mice -- Mice, Inbred BALB C -- Microspheres -- Polyesters -- Mus
Аннотация: An experimental dosage form of rubomycin is developed: the drug is incorporated in absorbable polymeric (polyhydroxybutyrate) matrix in the form of microparticles. Antitumor efficiency of this rubomycin dosage form was studied in laboratory mice with transplanted Ehrlich ascitic carcinoma. Rubomycin deposited in polymeric microparticles exhibited pronounced antitumor activity, inhibited the proliferative activity of Ehrlich ascitic carcinoma, and improved survival of mice with tumors. This dosage form of the drug can be used for local injections. В© Springer Science+Business Media, Inc. 2008.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
International Center for Studies of Critical Conditions, Presidium of Krasnoyarsk Research Center of Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Goreva, A.V.; Voinova, O.N.; Inzhevatkin, E.V.; Khlebopros, R.G.; Volova, T.G.

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Tissue reaction to intramuscular injection of resorbable polymer microparticles / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - P786-790, DOI 10.1007/s10517-007-0432-0 . - ISSN 0007-4888
Кл.слова (ненормированные):
Microencapsulation -- Polyhydroxybutyrate -- Resorbable polymers -- Tissue reaction -- poly(3 hydroxybutyric acid) -- polymer -- animal experiment -- animal tissue -- article -- cell infiltration -- controlled study -- drug delivery system -- drug formulation -- female -- giant cell -- inflammation -- macrophage -- microencapsulation -- nonhuman -- rat -- tissue reaction -- Absorbable Implants -- Animals -- Drug Compounding -- Female -- Foreign-Body Reaction -- Injections, Intramuscular -- Microspheres -- Polyesters -- Rats -- Rats, Wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Voinova, O.N.; Goreva, A.V.; Mogilnaya, O.A.; Volova, T.G.

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A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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10.

Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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11.

Rubomycin microincapsulation with biodegradable polymer matrix / E. I. Shishatskaya // Antibiotiki i Khimioterapiya. - 2005. - Vol. 50, Is. 8-9. - С. 4-9 . - ISSN 0235-2990
Кл.слова (ненормированные):
Daunorubicin (rubomycin) -- Microspheres -- Polyhydroxybutirate -- antineoplastic antibiotic -- daunorubicin -- hydroxybutyric acid -- microsphere -- poly(3 hydroxybutyric acid) -- poly-beta-hydroxybutyrate -- polyester -- polymer -- article -- chemistry -- delayed release formulation -- electronics -- methodology -- microcapsule -- temperature -- Antibiotics, Antineoplastic -- Capsules -- Daunorubicin -- Delayed-Action Preparations -- Hydroxybutyrates -- Microspheres -- Miniaturization -- Polyesters -- Polymers -- Temperature
Аннотация: A procedure for preparation of microspheres from biodegradable linear polyether of microbiological origin (polyhydroxybutirate, PHB) with using the technology of solvent evaporation was developed considering a specific example of two- and three-component emulsions. The procedure provided permanent preparation of the microspheres of high quality. The influence of the procedure (emulsion type, dispersion process and medium temperature) on the yield of the microspheres, their structure and size was shown. The temperature had a significant impact on incorporation of the antitumor anthracycline antibiotic daunorubicin (rubomycin) to the polymer matrix. The microspheres with various levels of the drug load (29 and 90% of the initial content in the emulsion) were prepared and the kinetics of the in vitro rubomycin release was studied. The dynamics of the highly toxic rubomycin release from the microspheres was on the whole even with the curve profile reaching the plateau in 20-22 hours of the observation period. The rate of the rubomycin release to the medium depended on the value of the antibiotic incorporation and was maximum within the first two hours (3.3 and 13.0 mcg/mlВ·h) that corresponded to the release of 0.97 and 3.89 of the incorporated antibiotic. The average rate of rubomycin release during 300 hours was 0.81В·10-4 and 2.3В·10-4 mcg/mlВ·h. The release constituted respectively 3.9 and 13.11% of the antibiotic incorporated to the microspheres.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.

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12.

Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - P719-728, DOI 10.1023/B:JMSM.0000030215.49991.0d . - ISSN 0957-4530
Кл.слова (ненормированные):
Necrosis -- Polymeric implants -- Suppurative inflammation -- Tissue reaction -- Biodegradation -- Calcification (biochemistry) -- Cells -- Copolymers -- Implants (surgical) -- Silk -- Tissue -- Tumors -- Materials science -- acid phosphatase -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polyhydroxyvaleric acid -- unclassified drug -- animal experiment -- animal model -- article -- biodegradable implant -- blood vessel reactivity -- catgut -- controlled study -- enzyme activity -- female -- giant cell -- histochemistry -- inflammation -- macrophage -- nonhuman -- priority journal -- rat -- silk -- suture -- tensile strength -- tissue reaction -- tissue structure -- wound healing -- young modulus -- Absorbable Implants -- Animals -- Female -- Fibrosis -- Foreign-Body Reaction -- Hydroxybutyrates -- Muscle, Skeletal -- Polyesters -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Treatment Outcome -- Wounds, Penetrating -- Animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.

Scopus
Держатели документа:
Inst. Biophys. Siberian Br. Russ. A., Akademgorodok, Krasnoyarsk 660036, Russia, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russia, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Puzyr, A.P.; Mogilnaya, O.A.; Efremov, S.N.

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13.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - P170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5 . - ISSN 0003-6838
Кл.слова (ненормированные):
acetyl coenzyme A acyltransferase -- bacterial enzyme -- carbon -- carbon dioxide -- fructose -- hydrogen -- hydroxybutyrate dehydrogenase -- oxidoreductase -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- synthetase -- article -- bacterial metabolism -- carbon source -- catalysis -- controlled study -- degradation -- depolymerization -- enzyme activity -- enzyme analysis -- molecular dynamics -- nonhuman -- protein function -- Ralstonia eutropha -- recording -- statistical significance -- synthesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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14.

Results of biomedical investigations of PHB and PHB/PHV fibers / T. Volova [et al.] // Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - P125-133, DOI 10.1016/S1369-703X(03)00038-X . - ISSN 1369-703X
Кл.слова (ненормированные):
Biocompatibility -- Fibers -- Implants -- Poly-3-hydroxybutyrate (PHB) -- Poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV) -- Polyhydroxyalkanoates (PHAs) -- Tissue reaction -- Bacteriology -- Calcification (biochemistry) -- Copolymers -- Flammability -- Tissue -- Tissue response -- Biomedical engineering -- acid phosphatase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid) -- polyhydroxyalkanoic acid -- suture material -- unclassified drug -- animal experiment -- article -- bacterial metabolism -- biochemistry -- biocompatibility -- biomedicine -- calcification -- catgut -- correlation analysis -- dose time effect relation -- enzyme activity -- fascia -- fiber -- foreign body -- functional assessment -- giant cell -- implantation -- in vivo study -- inflammation -- intermethod comparison -- macrophage -- materials testing -- multinuclear cell -- muscle injury -- necrosis -- nonhuman -- parameter -- phagocytosis -- physiology -- postoperative period -- priority journal -- process monitoring -- ralstonia eutropha b 5786 -- scar formation -- silk -- strength -- suture -- synthesis -- tissue reaction -- Wautersia eutropha -- wound healing -- Animalia -- Ralstonia -- Wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Inst. Transplantology Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Shishatskaya, E.; Sevastianov, V.; Efremov, S.; Mogilnaya, O.

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15.

Kinetic Parameters of a Culture of the Hydrogen-Oxidizing Bacterium Ralstonia eutropha Grown under Conditions Favoring Polyhydroxybutyrate Biosynthesis / T. G. Volova, N. A. Voinov // Applied Biochemistry and Microbiology. - 2003. - Vol. 39, Is. 2. - P166-170, DOI 10.1023/A:1022585912873 . - ISSN 0003-6838
Кл.слова (ненормированные):
hydrogen -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterium culture -- biosynthesis -- controlled study -- culture medium -- density -- fermentation -- gas -- gravimetry -- heat exchange -- nonhuman -- oxidation -- Ralstonia eutropha -- surface tension -- temperature dependence -- thermogenesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown on a gas substrate under conditions favoring autotrophic biosynthesis of polyhydroxybutyrate were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of the culture medium, and coefficients of heat emission and mass transfer.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660033, Russian Federation
Siberian State Technol. University, Min. of Educ. of the Russ. Fed., Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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16.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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17.

Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - P675-678, DOI 10.1007/s00253-002-0941-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
3 hydroxybutyric acid -- acetoacetyl coenzyme a reductase -- acetyl coenzyme A acyltransferase -- beta hydroxyvalerate -- butyrate dehydrogenase -- carbon monoxide -- electrolyte -- hydrogen -- oxidoreductase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid)synthase -- polyhydroxyalkanoic acid -- polymer -- unclassified drug -- valeric acid -- bacterium -- article -- autotrophy -- bacterial growth -- bacterial strain -- biomass production -- controlled study -- crystallization -- enzyme activity -- molecular weight -- nonhuman -- synthesis -- temperature -- Wautersia eutropha -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Lipids -- Polyesters -- Bacteria (microorganisms) -- Negibacteria -- Ralstonia -- Wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. Russian Academy of Sci., 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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18.

Cytotoxicity of polyhydroxyalkanoates in animal cell cultures. / E. I. Shishatskaya [et al.] // Doklady Biological Sciences. - 2000. - Vol. 374, Is. 1-6. - P539-542 . - ISSN 0012-4966
Кл.слова (ненормированные):
DNA -- hydroxybutyric acid -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3 hydroxybutyric acid) -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- poly-beta-hydroxybutyrate -- polyester -- RNA -- thymidine -- uridine -- animal -- article -- biosynthesis -- cell division -- cell strain 3T3 -- cell survival -- cytology -- drug effect -- liver -- liver cell -- metabolism -- mouse -- vascular endothelium -- 3T3 Cells -- Animals -- Cell Division -- Cell Survival -- DNA -- Endothelium, Vascular -- Hepatocytes -- Hydroxybutyrates -- Liver -- Mice -- Polyesters -- RNA -- Thymidine -- Uridine

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Eremeev, A.V.; Gitel'zon, I.I.; Setkov, N.A.; Volova, T.G.

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