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Вид документа : Статья из журнала
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Автор(ы) : Volova T., Zhila N., Kiselev E., Shishatskaya E.
Заглавие : A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers
Место публикации : Biotechnol. Prog.: John Wiley and Sons Inc., 2016. - Vol. 32, Is. 4. - С. 1017-1028. - ISSN 87567938 (ISSN) , DOI 10.1002/btpr.2267
Ключевые слова (''Своб.индексиров.''): biocompatibility--biosynthesis--molecular weight--physicochemical and mechanical properties--poly(3-hydroxybutyrate)/diethylene glycol copolymers--biochemistry--biocompatibility--biosynthesis--cell culture--cell membranes--cells--cytology--fatty acids--glycols--molecular weight--american institute of chemical engineers--biological properties--chemical compositions--cytoplasmic membrane--degree of saturations--physico-chemical and mechanical properties--physiological effects--poly-3-hydroxybutyrate--biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers
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