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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Goreva A.V., Voinova O.N., Kalacheva G.S., Volova T.G.
Заглавие : Distribution and resorption of polymeric microparticles in visceral organs of laboratory animals after intravenous injection
Место публикации : Bulletin of Experimental Biology and Medicine. - 2009. - Vol. 148, Is. 5. - С. 789-793. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-010-0817-3
Ключевые слова (''Своб.индексиров.''): 14c--intravenous injection--microparticles--resorbable polyhydroxybutyrate--visceral tissues--carbon 14--microsphere--polyhydroxyaminobutyric acid--polymer--unclassified drug--animal experiment--animal tissue--article--biocompatibility--biodegradability--body growth--controlled drug release--controlled study--degradation kinetics--drug delivery system--drug dosage form--experimental animal--female--liver parenchyma--molecular weight--nonhuman--particle size--physical development--radioactivity--rat--spleen--tissue distribution--tissue structure--animals--animals, laboratory--carbon radioisotopes--female--injections, intravenous--particle size--polymers--rats--rats, wistar--tissue distribution--viscera--animalia
Аннотация: Microparticles obtained by using 14C-labeled resorbable hydroxyaminobutyric acid polymer were injected into the caudal vein of laboratory animals without negative aftereffects for their growth and development and without changes in the macro- and microstructure of organs and tissues. The distribution of microparticles in the viscera and the dynamics of accumulation of carbon-containing polymer degradation products in the viscera were studied. The main targets for the particles are liver tissues, as well as renal and splenic tissues. The polymeric matrix of the microparticles is most actively destroyed in the spleen and liver. The presence of high-molecular- weight polymeric matrix in organs indicates the integrity of microparticles and the possibility of long-term (up to 12 weeks) functioning of polymeric particles in vivo. В© 2009 Springer Science+Business Media, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Goreva A.V., Voinova O.N., Inzhevatkin E.V., Khlebopros R.G., Volova T.G.
Заглавие : Evaluation of antitumor activity of rubomycin deposited in absorbable polymeric microparticles
Место публикации : Bulletin of Experimental Biology and Medicine. - 2008. - Vol. 145, Is. 3. - С. 358-361. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-008-0091-9
Ключевые слова (''Своб.индексиров.''): absorbable polymers--ehrlich's ascitic carcinoma--microencapsulation--polyhydroxybutyrate--rubomycin--poly(3 hydroxybutyric acid)--polymer--rubomycin--animal cell--animal experiment--animal model--antineoplastic activity--article--bagg albino mouse--cancer inhibition--cancer mortality--cancer survival--controlled study--drug delivery system--ehrlich ascites tumor--microencapsulation--mouse--nonhuman--polymerization--survival rate--tumor cell--tumor volume--absorption--animals--antibiotics, antineoplastic--carcinoma, ehrlich tumor--daunorubicin--hydroxybutyrates--mice--mice, inbred balb c--microspheres--polyesters--mus
Аннотация: An experimental dosage form of rubomycin is developed: the drug is incorporated in absorbable polymeric (polyhydroxybutyrate) matrix in the form of microparticles. Antitumor efficiency of this rubomycin dosage form was studied in laboratory mice with transplanted Ehrlich ascitic carcinoma. Rubomycin deposited in polymeric microparticles exhibited pronounced antitumor activity, inhibited the proliferative activity of Ehrlich ascitic carcinoma, and improved survival of mice with tumors. This dosage form of the drug can be used for local injections. В© Springer Science+Business Media, Inc. 2008.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A., Kalacheva G.S., Medvedeva S., Rodicheva E., Volova T.G.
Заглавие : Luminous bacteria as producers of polyhydroxyalkanoates
Место публикации : Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - С. 17-22. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.200850904
Ключевые слова (''Своб.индексиров.''): luminous bacteria--polyhydroxyalkanoates--polyhydroxybutyrate--abs resins--acids--bacteriology--batch cell culture--biological materials--biomass--biopolymers--biotechnology--cell culture--esters--hydrocarbons--organic compounds--polymers--renewable energy resources--supramolecular chemistry--batch cultures--dry cells--luminous bacteria--micro-organisms--photobacterium leiognathi--photobacterium phosphoreum--polyhydroxyalkanoates--polyhydroxybutyrate--polymer yields--vibrio fischeri--bioluminescence
Аннотация: The study addresses the ability of luminous bacteria of different taxa (Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio harveyi, Vibrio fischeri) to synthesize polyesters of hydrocarbon acids (polyhydroxyalkanoates, PHAs) as storage macromolecules. The screened strains widely varied in their PHA productivity. Conditions for attaining high polymer yields (including two- and three-component polymers) in batch culture have been determined. The attained polymer yields reached 40-70% of dry cell biomass. The results suggest a conclusion that luminous microorganisms can be considered as producers of multi-component PHAs. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Biocompatibility of polyhydroxybutyrate microspheres: In vitro and in vivo evaluation
Место публикации : Journal of Materials Science: Materials in Medicine. - 2008. - Vol. 19, Is. 6. - С. 2493-2502. - ISSN 09574530 (ISSN) , DOI 10.1007/s10856-007-3345-6
Ключевые слова (''Своб.индексиров.''): biocompatibility--drug delivery--fibroblasts--ion implantation--microspheres--polymer matrix--fibroblast cells--hydroxybutyric acid--intramuscular implantation--polyhydroxybutyrate--organic polymers--microsphere--poly(3 hydroxybutyric acid)--polymer--animal cell--animal experiment--animal tissue--article--biocompatibility--cell infiltration--controlled study--giant cell--implantation--in vitro study--in vivo study--inflammation--macrophage--mouse--nonhuman--priority journal--rat--3-hydroxybutyric acid--animals--biocompatible materials--cell survival--inflammation--materials testing--mice--microspheres--necrosis--nih 3t3 cells--polymers--rats--rats, wistar--tetrazolium salts--thiazoles--time factors--rattus norvegicus
Аннотация: Microspheres have been prepared from the resorbable linear polyester of ?-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection. В© 2008 Springer Science+Business Media, LLC.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Tissue reaction to intramuscular injection of resorbable polymer microparticles
Место публикации : Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - С. 786-790. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-007-0432-0
Ключевые слова (''Своб.индексиров.''): microencapsulation--polyhydroxybutyrate--resorbable polymers--tissue reaction--poly(3 hydroxybutyric acid)--polymer--animal experiment--animal tissue--article--cell infiltration--controlled study--drug delivery system--drug formulation--female--giant cell--inflammation--macrophage--microencapsulation--nonhuman--rat--tissue reaction--absorbable implants--animals--drug compounding--female--foreign-body reaction--injections, intramuscular--microspheres--polyesters--rats--rats, wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Khlusov I.A., Volova T.G.
Заглавие : A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - С. 481-498. - ISSN 09205063 (ISSN) , DOI 10.1163/156856206776986242
Ключевые слова (''Своб.индексиров.''): biocompatibility--hydroxyapatite (ha)--phb-hydroxyapatite composite--polyhydroxyalkanoate (pha)--polyhydroxybutyrate (p(3hb))--properties--biocompatibility--differential thermal analysis--electron microscopy--free energy--interfacial energy--physical properties--surface properties--x ray analysis--biomedical application--physicochemical properties--polyhydroxyalkanoate (pha)--polyhydroxybutyrate (phb)--hydroxyapatite--hydroxyapatite--poly(3 hydroxybutyric acid)--polymer--biomaterial--hydroxybutyric acid--adhesion--animal cell--animal tissue--article--biomedicine--bone marrow cell--cell differentiation--cell growth--chemical structure--composite material--controlled study--crystallization--decomposition--electron microscopy--in vitro study--in vivo study--melting point--mouse--nonhuman--ossification--osteoblast--physical chemistry--priority journal--rat--strength--structure analysis--surface property--synthesis--temperature measurement--thermal analysis--tissue engineering--wettability--animal--biomechanics--bioremediation--bone prosthesis--cattle--cell culture--chemistry--cytology--differential scanning calorimetry--drug effect--human--materials testing--prostheses and orthoses--scanning electron microscopy--standard--wistar rat--murinae--animals--biocompatible materials--biodegradation, environmental--biomechanics--bone substitutes--cattle--cells, cultured--differential thermal analysis--durapatite--humans--hydroxybutyrates--materials testing--microscopy, electron, scanning--osteoblasts--prostheses and implants--rats--rats, wistar--surface properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gordeev S.A., Puzyr A.P.
Заглавие : Degradation of P(3HB) and P(3HB-co-3HV) in biological media
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - С. 643-657. - ISSN 09205063 (ISSN) , DOI 10.1163/1568562053783678
Ключевые слова (''Своб.индексиров.''): degradation rate--fiber properties--morphology--poly(hydroxybutyrate-co-hydroxyvalerate) (p(3hb-co-3hv))--polyhydroxyalkanoates(phas)--polyhydroxybutyrate (p(3hb))--copolymers--degradation--differential scanning calorimetry--enzymes--morphology--scanning electron microscopy--tensile strength--tissue--transmission electron microscopy--degradation rate--fiber properties--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (p(3hb))--biopolymers--buffer--copolymer--poly(3 hydroxybutyric acid)--polyhydroxybutyrate hydroxyvalerate copolymer--unclassified drug--animal experiment--animal model--animal tissue--article--biodegradation--controlled study--crystal structure--fiber--giant cell--in vitro study--in vivo study--macrophage--morphology--nonhuman--ph--priority journal--rat--structure analysis--tensile strength--tissue water--weight reduction--animals--biodegradation, environmental--buffers--humans--hydrogen-ion concentration--hydroxybutyrates--macrophages--microscopy, electron, scanning--microscopy, electron, transmission--muscle, skeletal--polyesters--rats--rats, wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Plotnikov V.F., Shishatskaya E.I., Mironov P.V., Vasil'ev A.D.
Заглавие : Physicochemical properties of two-component polyhydroxyalkanoates, 3-hydroxybutyrate-3-hydroxyvalerate copolymers
Место публикации : Biophysics. - 2004. - Vol. 49, Is. 6. - С. 934-942. - ISSN 00063509 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydroxybutyrate-hydroxyvalerate copolymers--physicochemical properties--polyhydroxyalkanoates--structure--bacteria (microorganisms)--cupriavidus necator
Аннотация: A series of two-component polyhydroxyalkanoates composed of hydroxybutyrate-hydroxyvalerate copolymers with different monomer ratio was obtained with the use of bacteria Ralstonia eutropha B5786. The properties of the polyhydroxyalkanoates in comparison with the homopolymer of hydroxybutyric acid were studied by X-ray diffraction analysis, IR spectroscopy, differential thermal analysis, and viscometry. The ratio of crystalline to amorphous phase in the copolymers tends to unity with increasing hydroxyvalerate content. This is accompanied by a decrease in the degree of crystallinity of the copolymers from 70-80 to 45-50%, the dependence is virtually linear within the range, of hydroxyvalerate mole fraction from several to 25-30 mol%. Thermal characteristics, melting temperature (Tm) and decomposition temperature (Td), of the polyhydroxyalkanoate copolymers are lower than those for polyhydroxybutyrate, whose Tm and Td are 168-170 and 260-265В°C, respectively. Both parameters decrease to 150-160 and 200-220В°C, respectively, when the hydroxyvalerate mole fraction is raised. No distinct correlation between polymer composition and molecular weight has been revealed. Copyright В© 2004 by MAIK "Nauka/Interperiodica".
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Место публикации : Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - С. 719-728. - ISSN 09574530 (ISSN) , DOI 10.1023/B:JMSM.0000030215.49991.0d
Ключевые слова (''Своб.индексиров.''): necrosis--polymeric implants--suppurative inflammation--tissue reaction--biodegradation--calcification (biochemistry)--cells--copolymers--implants (surgical)--silk--tissue--tumors--materials science--acid phosphatase--copolymer--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polyhydroxyvaleric acid--unclassified drug--animal experiment--animal model--article--biodegradable implant--blood vessel reactivity--catgut--controlled study--enzyme activity--female--giant cell--histochemistry--inflammation--macrophage--nonhuman--priority journal--rat--silk--suture--tensile strength--tissue reaction--tissue structure--wound healing--young modulus--absorbable implants--animals--female--fibrosis--foreign-body reaction--hydroxybutyrates--muscle, skeletal--polyesters--polymers--rats--rats, wistar--sutures--treatment outcome--wounds, penetrating--animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Gorbunova O.V., Zhila N.O.
Заглавие : Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786
Место публикации : Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - С. 170-177. - ISSN 00036838 (ISSN) , DOI 10.1023/B:ABIM.0000018921.04863.d5
Ключевые слова (''Своб.индексиров.''): acetyl coenzyme a acyltransferase--bacterial enzyme--carbon--carbon dioxide--fructose--hydrogen--hydroxybutyrate dehydrogenase--oxidoreductase--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--synthetase--article--bacterial metabolism--carbon source--catalysis--controlled study--degradation--depolymerization--enzyme activity--enzyme analysis--molecular dynamics--nonhuman--protein function--ralstonia eutropha--recording--statistical significance--synthesis--bacteria (microorganisms)--ralstonia--wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Gorbunova O.V., Zhila N.O.
Заглавие : Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha
Место публикации : Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 2. - С. 201-209. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): acetoacetyl coenzyme a reductase--acetoacetyl-coa reductase--acetyl coenzyme a acyltransferase--acyltransferase--alcohol dehydrogenase--carboxylesterase--hydroxybutyrate dehydrogenase--hydroxybutyric acid--poly(3 hydroxyalkanoic acid) depolymerase--poly(3-hydroxyalkanoic acid) depolymerase--poly(3-hydroxyalkanoic acid) synthase--polyhydroxyalkanoate synthase--polymer--article--chemistry--comparative study--culture medium--enzymology--growth, development and aging--metabolism--wautersia eutropha--acetyl-coa c-acyltransferase--acyltransferases--alcohol oxidoreductases--carboxylic ester hydrolases--culture media--cupriavidus necator--hydroxybutyrate dehydrogenase--hydroxybutyrates--polymers
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of the key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, D-hydroxybutyrate dehydrogenase, and PHA depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase were recorded at the stage of acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only at stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not cause any marked changes in the time course of enzyme activity.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - С. 1029-1042. - ISSN 09205063 (ISSN) , DOI 10.1163/156856203769231547
Ключевые слова (''Своб.индексиров.''): ?-hydroxy acids--endotoxins--hemocompatibility--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--bacterium lipopolysaccharide--carbon--complement--copolymer--hydroxyacid--long chain fatty acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--valeric acid derivative--adult--article--biofilm--biotechnology--blood analysis--blood clotting--blood compatibility--cell function--chemical analysis--chemical composition--complement activation--concentration (parameters)--controlled study--gas chromatography--hemostasis--human--human cell--mass spectrometry--micromorphology--nonhuman--priority journal--purification--quantitative analysis--sampling--synthesis--thrombocyte adhesion--wautersia eutropha--biocompatible materials--blood--blood coagulation tests--chromatography, gas--complement activation--cupriavidus necator--fatty acids--humans--platelet adhesiveness--polyesters--surface properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Kinetic Parameters of a Culture of the Hydrogen-Oxidizing Bacterium Ralstonia eutropha Grown under Conditions Favoring Polyhydroxybutyrate Biosynthesis
Место публикации : Applied Biochemistry and Microbiology. - 2003. - Vol. 39, Is. 2. - С. 166-170. - ISSN 00036838 (ISSN) , DOI 10.1023/A:1022585912873
Ключевые слова (''Своб.индексиров.''): hydrogen--poly(3 hydroxybutyric acid)--article--bacterial growth--bacterium culture--biosynthesis--controlled study--culture medium--density--fermentation--gas--gravimetry--heat exchange--nonhuman--oxidation--ralstonia eutropha--surface tension--temperature dependence--thermogenesis--bacteria (microorganisms)--ralstonia--wautersia eutropha
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown on a gas substrate under conditions favoring autotrophic biosynthesis of polyhydroxybutyrate were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of the culture medium, and coefficients of heat emission and mass transfer.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Kinetic parameters of a culture of the hydrogen-oxidizing Ralstonia eutropha, grown under the regimen of biosynthesis of polyhydroxybutyrate
Место публикации : Prikladnaia biokhimiia i mikrobiologiia. - 2003. - Vol. 39, Is. 2. - С. 189-193. - ISSN 05551099 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydrogen--hydroxybutyric acid--polyester--article--culture medium--enzyme specificity--growth, development and aging--kinetics--metabolism--oxidation reduction reaction--temperature--wautersia eutropha--culture media--cupriavidus necator--hydrogen--hydroxybutyrates--kinetics--oxidation-reduction--polyesters--substrate specificity--temperature
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha, grown on a gas substrate under the conditions favoring autotrophic biosynthesis of polyhydroxybutyrate, were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of culture medium, and coefficients of heat emission and mass transfer.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogil'naya O.A., Efremov S.N., Gitelson I.I.
Заглавие : Tissue morphogenesis under the conditions of implantation of polyhydroxybutyrate, a biodegradable polymer.
Место публикации : Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - С. 123-126. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): biomaterial--hydroxybutyric acid--polymer--animal--article--bioremediation--female--morphogenesis--rat--wistar rat--animals--biocompatible materials--biodegradation, environmental--female--hydroxybutyrates--morphogenesis--polymers--rats--rats, wistar
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beliaev G.A., Volova T.G., Drokin N.A., Shepov V.N.
Заглавие : Study of the dielectric permeability in the superhigh frequency range of a degraded polyoxybutyrate biopolymer
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 4. - С. 636-640. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): biopolymer--hydroxybutyric acid--alcaligenes--article--chemistry--electric conductivity--radiofrequency radiation--alcaligenes--biopolymers--electric conductivity--hydroxybutyrates--radio waves
Аннотация: The dielectric permeability of the degradable biopolymer polyhydroxybutyrate synthesized by hydrogen-oxidizing bacteria Alcaligenes eutrophus was investigated by the resonance method using original highly sensitive microstrip sensors. For the first time, a linear growth of dielectric permeability (delta epsilon/delta T = 7 x 10(-4) degree-1) due to the flexibility of the polymer chain in the temperature range from 10 to 70 degrees C was revealed. The energy of a bend of the nearest fragments was evaluated (E = 392 K), and its correspondence to the energies of bends of the alcyl groups of low-molecular substances like liquid crystals was established. It was shown that at low values of dielectric permeability in the high-frequency range (epsilon' = 2.25 +/- 0.02), which are stable, in a wide range of frequencies of the electromagnetic field (1 MHz - 1 Hz), polyoxybutyrate can be used in the microwave equipment.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Vasil'ev A.D., Zeer E.P., Petrakovskaia E.A., Falaleev O.V.
Заглавие : Study of molecular structure of polyhydroxybutyrate-a termoplastic anddegradable biopolymer
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 3. - С. 445-451. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon--hydroxybutyric acid--polyester--alcaligenes--article--chemistry--crystallization--alcaligenes--carbon--crystallization--hydroxybutyrates--polyesters
Аннотация: The molecular structure of polyhydroxybutyrate from hydrogen-oxidizing bacteria Alcaligenes eutrophus was studied by X-ray diffraction analysis. It was shown that the degree of crystallinity of various samples depends little on the conditions of their preparation and is equal to 0.62-0.76. The molecular structure of solid samples and solution of polyhydroxybutyrate in chloroform were studied by the NMR and EPR methods. The conclusion is made that the molecular structure of polyhydroxybutyrate does not depend on the features of the strain and conditions of carbon nutrition of microorganisms producing polyhydroxybutyrate. Defects induced by gamma-radiation in polyhydroxybutyrate were studied. Free radicals were isolated, and their structure was decoded.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belyaev B.A., Volova T.G., Drokin N.A., Shepov V.N.
Заглавие : The SHF dielectric permeability of polyhydroxybutyrate, a degradable biological polymer.
Место публикации : Doklady Biophysics. - 2000. - Vol. 370-372. - С. 9-12. - ISSN 00124974 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydroxybutyric acid--polymer--article--chemistry--electricity--electromagnetic field--temperature--electromagnetic fields--electrostatics--hydroxybutyrates--polymers--temperature
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Goreva A., Kalacheva G., Volova T.
Заглавие : Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - С. 2185-2203. - ISSN 09205063 (ISSN) , DOI 10.1163/092050610X537138
Ключевые слова (''Своб.индексиров.''): 14c products of polymer resorption--intravenous administration--microparticles--resorbable polyhydroxybutyrate--tissues of internal organs--3-hydroxybutyric acid--sup14/supc products of polymer resorption--adverse effect--drug-delivery systems--growth and development--high molecular weight--initial molecular weight--initial values--internal organs--intravenous administration--laboratory animals--local response--matrix--mean diameter--micro-particles--polymer degradation--polymer microparticles--preparation process--radioactivity level--residual content--resorbable--sustained-release--tail veins--wistar rat--animals--biocompatibility--degradation--histology--molecular weight--morphology--radiation--radioactivity--rats--tissue--poly(3 hydroxybutyric acid)--animal experiment--animal tissue--article--biocompatibility--concentration process--controlled study--degradation--drug delivery system--female--gel permeation chromatography--heart--kidney parenchyma--liver--lung parenchyma--molecular weight--nonhuman--organ culture--priority journal--radioactivity--rat--spleen--animals--animals, laboratory--biocompatible materials--drug delivery systems--female--infusions, intravenous--materials testing--microspheres--molecular weight--particle size--polymers--rats--rats, wistar--tissue distribution--animalia--rattus--rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Plotnikov V.F., Puzyr' A.P.
Заглавие : Studies of Biodegradation of Microbial Polyhydroxyalkanoates
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1998. - Vol. 34, Is. 5. - С. 543. - ISSN 05551099 (ISSN)
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.
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