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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Murueva A. V., Shershneva A. M., Abanina K. V., Prudnikova S. V., Shishatskaya E. I.
Заглавие : Development and characterization of ceftriaxone-loaded P3HB-based microparticles for drug delivery
Место публикации : Dry. Technol.: Taylor and Francis Inc., 2018. - Article in press. - ISSN 07373937 (ISSN) , DOI 10.1080/07373937.2018.1487451
Ключевые слова (''Своб.индексиров.''): ceftriaxone--drug release--emulsification technique--microbiological evaluation--p3hb-microparticles--spray-drying--drying--emulsification--encapsulation--functional polymers--spray drying--targeted drug delivery--anti-bacterial activity--ceftriaxone--double emulsifications--drug release--encapsulation efficiency--micro-particles--microbiological evaluation--polymeric microparticles--controlled drug delivery
Аннотация: In this study, polymer-based microparticles are used to improve the therapeutic properties of ceftriaxone (CEF) and render them safer. Poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (P3HB-PEG)-based microparticles were prepared by two methods: a double emulsification technique and spray-drying. The microparticles were characterized in terms of size and zeta potential, morphology, total drug loading and drug release. The microparticles had spherical shapes with diameters of a size range from 0.74 to 1.55 µm (emulsification technique) and from 3.84 to 6.51 µm (spray-drying); CEF encapsulation efficiency was around 63% and 49% for these methods respectively. The CEF release from microparticles obtained by spray-drying reached 100% after 150 h, while for microparticles obtained by emulsification technique the total release of CEF did not exceed 34% after 312 h. The release profiles could be best explained by Zero order kinetics model, Higuchi and Korsmeyer-Peppas models, as the plots showed high linearity. Antibacterial activity of the microparticles was evaluated against gram-positive and gram-negative bacterial strains. In general, CEF encapsulation in polymeric microparticles preserves the therapeutic efficacy of the CEF and provides its prolonged effect. © 2018, © 2018 Taylor & Francis.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Menshikova O., Zhila N., Vasiliev A., Kiselev E., Peterson I., Shishatskaya E., Thomas S.
Заглавие : Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646
Место публикации : Journal of Chemical Technology and Biotechnology. - 2018. - Article in press. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.5763
Ключевые слова (''Своб.индексиров.''): 3-hydroxy-4-methylvalerate--physicochemical properties--polyhydroxyalkanoates--synthesis--biochemistry--chemical industry--synthesis (chemical)--3-hydroxy-4-methylvalerate--crystalline-to-amorphous--degree of crystallinity--degrees of crystallinity--physicochemical property--polyhydroxyalkanoates--processing properties--wild-type strain--polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova N. G., Pyatina S. А., Nikolaeva E. D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov D. B., Sakhnov E. V., Shishatskaya E. I.
Заглавие : Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients
Место публикации : Toxicol. Rep.: Elsevier Inc., 2019. - Vol. 6. - С. 74-90. - ISSN 22147500 (ISSN) , DOI 10.1016/j.toxrep.2018.11.009
Ключевые слова (''Своб.индексиров.''): atherosclerosis--cell morphology--intravascular stenting--macrophages--monocytes--polyhydroxyalkanoates
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients. © 2018 The Authors
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Автор(ы) : Volova T. G., Vinnik Y. S., Shishatskaya E. I., Markelova N. M., Zaikov G. E.
Заглавие : Natural-based polymers for biomedical applications
Место публикации :: Apple Academic Press, 2017. - С. 1-439. - , DOI 10.1201/9781315366036
Аннотация: This new book presents the authors’ biomedical studies of natural degradable biopolymers (polyhydroxyalkanoates [PHAs]) and discusses the demand for medical-grade materials and modern trends, focusing on the present status and future potential of PHAs. The authors present and summarize their most important results and findings obtained during the last few years in experimental studies and clinical trials of PHAs at the Institute of Biophysics Siberian Branch of Russian Academy of Science. © 2017 by Apple Academic Press, Inc.
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Вид документа :
Шифр издания :
Автор(ы) : Volova T. G., Goncharov D. B., Nikolaeva E. D., Shishatskaya E. I.
Заглавие : Electrospinning of degradable phas: Process, properties, applications
Место публикации :: Nova Science Publishers, Inc., 2017. - С. 1-56
Ключевые слова (''Своб.индексиров.''): biological and physical/mechanical properties--cell proliferation--electrospinning--nonwoven membranes--pha--scaffolds--skin regeneration--ultrafine fibers--wound dressings
Аннотация: An integrated study has been performed to investigate the process of formation of ultrafine fibers and nonwoven membranes by electrospinning from natural degradable polymers-polyhydroxyalkanoates (PHAs); physical, mechanical, and biological properties of the products have been studied. Then, electrospinning was used to prepare ultrafine fibers from PHAs with different compositions: P(3HB) and its copolymers P(3HB-co-4HB), P(3HB-co-3HV), and P(3HB-co-3HHx). The main process parameters, that influence UF-fiber diameter and properties of fibrous non-woven membranes) (polymer concentration, solution feeding rate, working distance, and applied voltage), were investigated and their effects evaluated. This study was the first to compare biological and physical/mechanical parameters of PHAs with different chemical compositions as dependent upon the fractions of monomers, constituting the polymers and fiber orientation. Electrospun polymer membranes, prepared from the [P(3HB-co-4HB)], were tested as wound dressings. The developed nonwoven membranes can be used as the equivalent of collagen skine dressings in the treatment of burns of degree II. Experiments on laboratory animals with model skin defects showed, that the membranes fitted the wound shape good, protected the wound from external influences, and facilitated wound healing, promoting fast repair. The successful experiments on laboratory animals were followed by pilot clinical trials of nanomembranes, comprised of PHA membranes as wound dressings in the treatment of septic wounds. During the regeneration phase, PHA membranes served as a scaffold for the new tissue on the skin and filled out soft tissue defects. The formation of the uniform and sufficiently vascularized tissue is a prerequisite for quicker wound healing and can serve as a basis for the subsequent skin grafting and spontaneous re-epithelialization of superficial wounds. The wound dressing, tested in this clinical trial, performs important physiological functions of natural skin, provides a barrier against secondary infection, reduces fluid loss, and, at the same time, does not keep the air out. © 2017 Nova Science Publishers, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Shidlovskiy I. P., Nikolaeva E. D., Sukovatiy A. G., Vasiliev A. D., Shishatskaya E. I.
Заглавие : Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics
Место публикации : Polym Test: Elsevier Ltd, 2018. - Vol. 65. - С. 54-68. - ISSN 01429418 (ISSN) , DOI 10.1016/j.polymertesting.2017.10.023
Ключевые слова (''Своб.индексиров.''): antibacterial activity--antibiotics--bacterial cellulose--composites--properties--silver nanoparticles--antibiotics--atoms--boron carbide--cell culture--cellulose--cellulose films--composite materials--escherichia coli--materials testing apparatus--metal nanoparticles--nanocomposite films--nanoparticles--scanning electron microscopy--silver compounds--spectrum analysis--synthesis (chemical)--tensile testing--water pollution--x ray analysis--anti-bacterial activity--antibacterial properties--bacterial cellulose--mechanical characteristics--properties--silver nanoparticles--structure and properties--tensile testing machines--silver--antibiotics--cellulose--composites--properties--silver
Аннотация: The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO3: as AgNO3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones. © 2017 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lonshakova-Mukina V. I., Esimbekova E. N., Kratasyuk V. A.
Заглавие : Stabilization of Butyrylcholinesterase by the Entrapment into the Natural Polymer-Based Gels
Место публикации : Doklad. Biochem. Biophys.: Pleiades Publishing, 2018. - Vol. 479, Is. 1. - С. 98-100. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672918020126
Аннотация: A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE, which allowed us to simplify the procedure and reduce the time of analysis of organophosphorus pesticides. The resulting immobilized samples retained activity for at least 300 days. BuChE samples based on the starch gel showed a greater sensitivity in the determination of pesticides as compared to the samples based on the gelatin gel. © 2018, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila, Natalia, Shishatskaya, Ekaterina
Заглавие : Properties of PHA bi-, ter-, and quarter-polymers containing 4-hydroxybutyrate monomer units
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [17-15-01352]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER SCIENCE BV, 2018. - Vol. 111. - С. 1019-1026. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2018.01.130. - ISSN 1879-0003(eISSN)
Примечания : Cited References:39. - The research was supported by the Russian Science Foundation (grant No. 17-15-01352).
Предметные рубрики: ALCALIGENES-FAECALIS T1
COMAMONAS-ACIDOVORANS
ENZYMATIC DEGRADATION
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates--4-hydroxybutyrate monomer units--physicochemical--and mechanical properties
Аннотация: The present study investigates physicochemical, mechanical, and biological properties of polyhydroxyalkanoate (PHA) copolymers containing 4-hydroxybutyrate (4HB) synthesized in Cupriavidus eutrophus B10646 culture. In poly(3-hydroxybutyrate/4-hydroxybutyrate) [P(3HB/4HB)]bipolymers, 4HB varied between 10.4 and 75.0 mol%; in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate) terpolymers, 4HB constituted 28.7-55.6 mol%; and in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate/3-hydroxyhexanoate) quaterpolymers, 4HB varied between 9.3 and 13.3 mol%. The degree of crystallinity of P(3HB/4HB) copolymers decreased consistently with an increase in 4HB content, reaching 38%. The incorporation of 3-hydroxyvalerate and 3-hydroxyhexanoate into copolymers enhanced that effect. The effect of 4HB monomer units on temperature properties of copolymers was exhibited as lowering of the melting temperature and crystallization temperature, which improved the processing-related properties of the copolymers. All copolymers containing 4HB showed enhanced elongation at break compared to poly(3-hydroxybutyrate). Polymer films prepared from PHA5 with different chemical composition had similar microstructure and porosity and had no toxic effect on mouse fibroblast NIH 3 T3 cells, proving their high biocompatibility. (C) 2018 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Surmenev R. A., Chernozem R. V., Syromotina D. S., Oehr C., Baumbach T., Krause B., Boyandin A. N., Dvoinina L. M., Volova T. G., Surmeneva M. A.
Заглавие : Low-temperature argon and ammonia plasma treatment of poly-3-hydroxybutyrate films: Surface topography and chemistry changes affect fibroblast cells in vitro
Место публикации : Eur Polym J: Elsevier Ltd, 2019. - Vol. 112. - С. 137-145. - ISSN 00143057 (ISSN) , DOI 10.1016/j.eurpolymj.2018.12.040
Ключевые слова (''Своб.индексиров.''): cell adhesion--dielectric barrier discharge--plasma treatment--surface etching--wetting behaviour--ammonia--argon--cell adhesion--cell culture--chemical modification--contact angle--dielectric materials--electric discharges--fibroblasts--mammals--nitrogen plasma--plasma applications--surface roughness--surface treatment--temperature--topography--ammonia plasma treatment--dielectric barrier discharges--different proportions--plasma treatment--poly-3-hydroxybutyrate--poly3-hydroxybutyrate (phb)--surface etching--wetting behaviour--wetting
Аннотация: Poly-3-hydroxybutyrate (PHB) films were plasma-treated using pure NH3, pure Ar or mixtures of the two different proportions (20%, 30%, 40%, 50%, 70% NH3 in Ar). Surface chemistry and surface topography changes of PHB films were observed after plasma processing in all plasma regimes. The XPS results confirmed the absence of chemical modification in the case of pure Ar plasma treatment. Nitrogen-containing groups (e.g., N–C[dbnd]O) were detected on the surfaces of P3HB films treated with NH3-containing plasma. The surfaces of the untreated P3HB films were hydrophobic, and plasma treatment turned the surfaces hydrophilic, irrespective of the treatment. A significant decrease in the contact angle and an increase in the free surface energy were observed. An insignificant surface ageing effect was observed when P3HB samples were exposed to air for 10 days. In NIH 3T3 mice fibroblast cells, cell adhesion was significantly improved after plasma treatment in an Ar atmosphere, which is likely related to the fact that there was a surface ? potential of 88.6 mV at neutral pH, causing a cleavage of the polymer chains and an increase in surface roughness. © 2018 Elsevier Ltd
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kiselev, Evgeniy G., Boyandin, Anatoly N., Zhila, Natalia O., Prudnikova, Svetlana, V, Shumilova, Anna A., Baranovskiy, Sergey, V, Shishatskaya, Ekaterina, I, Thomas, Sabu, Volova, Tatiana G.
Заглавие : Constructing sustained-release herbicide formulations based on poly-3-hydroxybutyrate and natural materials as a degradable matrix
Колич.характеристики :14 с
Коллективы : Government of the Russian Federation [074-02-2018-328, 220]
Место публикации : Pest Manag. Sci.: JOHN WILEY & SONS LTD. - ISSN 1526-498X, DOI 10.1002/ps.5702. - ISSN 1526-4998(eISSN)
Примечания : Cited References:83. - This study was financially supported by the project 'Agropreparations of the new generation: a strategy of construction and realization' (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, 'On measures designed to attract leading scientists to the Russian institutions of higher learning'.
Предметные рубрики: SOIL MICROBIAL COMMUNITY
FENOXAPROP-P-ETHYL
SLOW-RELEASE
METRIBUZIN
Аннотация: BACKGROUND The purpose of the present study was to develop ecofriendly herbicide formulations. Its main aim was to develop and investigate slow-release formulations of herbicides (metribuzin, tribenuron-methyl, and fenoxaprop-P-ethyl) of different structure, solubility, and specificity, which were loaded into a degradable matrix of poly-3-hydroxybutyrate (P(3HB)) blended with available natural materials (peat, clay, and wood flour). RESULTS Differences in the structure and physicochemical properties of the formulations were studied depending on the type of the matrix. Herbicide release and accumulation in soil were associated with the solubility of the herbicide. Fourier-transform infrared spectroscopy showed that no chemical bonds were formed between the components in the experimental formulations. Degradation of the formulations in agro-transformed soil in laboratory conditions was chiefly influenced by the shape of the specimens (granules or pellets) while the effect of the type of filler (peat, clay, or wood flour) was insignificant. The use of granules enabled more rapid accumulation of the herbicides in soil: their peak concentrations were reached after 3 weeks of incubation while the concentrations of the herbicides released from the pellets were the highest after 5-7 weeks. Loading of the herbicides into the polymer matrix composed of the slowly degraded P(3HB) and natural materials enabled both sustained function of the formulations in soil (lasting between 1.5 and = 3 months) and stable activity of the otherwise rapidly inactivated herbicides such as tribenuron-methyl and fenoxaprop-P-ethyl. CONCLUSION The experimental herbicide formulations enabled slow release of the active ingredients to soil. (c) 2019 Society of Chemical Industry
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Thomas S., Shumilova A. A., Kiselev E. G., Baranovsky S. V., Vasiliev A. D., Nemtsev I. V., Kuzmin A. P., Sukovatyi A. G., Avinash R. P., Volova T. G.
Заглавие : Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Article in press. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.11.112
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour – were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P(3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P(3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. © 2018
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kiselev E. G., Boyandin A. N., Zhila N. O., Prudnikova S. V., Shumilova A. A., Baranovskiy S. V., Shishatskaya E. I., Thomas S., Volova T. G.
Заглавие : Constructing sustained-release herbicide formulations based on poly-3-hydroxybutyrate and natural materials as a degradable matrix
Место публикации : Pest Manage. Sci.: John Wiley and Sons Ltd, 2019. - Article in press. - ISSN 1526498X (ISSN), DOI 10.1002/ps.5702
Аннотация: BACKGROUND: The purpose of the present study was to develop ecofriendly herbicide formulations. Its main aim was to develop and investigate slow-release formulations of herbicides (metribuzin, tribenuron-methyl, and fenoxaprop-P-ethyl) of different structure, solubility, and specificity, which were loaded into a degradable matrix of poly-3-hydroxybutyrate (P(3HB)) blended with available natural materials (peat, clay, and wood flour). RESULTS: Differences in the structure and physicochemical properties of the formulations were studied depending on the type of the matrix. Herbicide release and accumulation in soil were associated with the solubility of the herbicide. Fourier-transform infrared spectroscopy showed that no chemical bonds were formed between the components in the experimental formulations. Degradation of the formulations in agro-transformed soil in laboratory conditions was chiefly influenced by the shape of the specimens (granules or pellets) while the effect of the type of filler (peat, clay, or wood flour) was insignificant. The use of granules enabled more rapid accumulation of the herbicides in soil: their peak concentrations were reached after 3 weeks of incubation while the concentrations of the herbicides released from the pellets were the highest after 5–7 weeks. Loading of the herbicides into the polymer matrix composed of the slowly degraded P(3HB) and natural materials enabled both sustained function of the formulations in soil (lasting between 1.5 and ?3 months) and stable activity of the otherwise rapidly inactivated herbicides such as tribenuron-methyl and fenoxaprop-P-ethyl. CONCLUSION: The experimental herbicide formulations enabled slow release of the active ingredients to soil. © 2019 Society of Chemical Industry. © 2019 Society of Chemical Industry
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya, Ekaterina I., Menzyanova, Natalia G., Shumilova, Anna A.
Заглавие : The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response
Колич.характеристики :11 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [17-15-01352]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER, 2019. - Vol. 141. - С. 765-+. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2019.09.015. - ISSN 1879-0003(eISSN)
Примечания : Cited References:57. - The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The study is supporting by the Russian Science Foundation, Project No 17-15-01352.
Предметные рубрики: 3-HYDROXYBUTYRATE
BIOCOMPATIBILITY
BIOSYNTHESIS
ADHESION
STENTS
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3 hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4 hydroxybutyrate (4HB), 3 hydroxyvalerate (3HV), or 3 hydroxyhexanoate (3HHx). All copolymers had the M-w of about 550-670 kDa, and the homopolymer P(3HB) had a significantly higher M-w - 920 kDa. P(3HB co 4HB) and P(3HB co 3HHx) had the lowest C-x (42 and 49%) while P(3HB co 3HV) and P(3HB) exhibited higher C-x values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71-75 nm, than the copolymer films, which were hydrophilic (57-60 degrees) and had considerably higher roughness (158-177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4 hydroxybutyrate and 3 hydroxyhexanoate were the best for contact with blood. (C) 2019 Elsevier B.V. All rights reserved.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Nikolaeva E. D., Kirichenko A. K., Shishatskaya E. I.
Заглавие : Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 230-240. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.068
Примечания : Cited By :1
Ключевые слова (''Своб.индексиров.''): bacterial cellulose--composites--polyhydroxyalkanoates--3 hydroxybutyric acid 4 hydroxybutyric acid copolymer--actovegin--bacterial cellulose--cellulose--collagen type 1--copolymer--cytokeratin 10--cytokeratin 14--solcoseryl--unclassified drug--angiogenesis--animal cell--animal experiment--animal model--animal tissue--biochemical analysis--biocompatibility--burn--cell growth--controlled study--cutaneous parameters--differential scanning calorimetry--drug release--elemental analysis--female--fibroblast--histopathology--in vitro study--in vivo study--inflammation--mechanics--nonhuman--rat--review--wound healing--wound planimetry--x ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shershneva A. M., Murueva A. V., Zhila N. O., Volova T. G.
Заглавие : Antifungal activity of P3HB microparticles containing tebuconazole
Место публикации : J. Environ. Sci. Health Part B Pestic. Food Contamin. Agric. Wastes: Taylor and Francis Inc., 2019. - Article in press. - ISSN 03601234 (ISSN) , DOI 10.1080/03601234.2018.1550299
Ключевые слова (''Своб.индексиров.''): antifungal activity--encapsulation--microparticles--poly-3-hydroxybutyrate--tebuconazole--agricultural chemicals--emulsification--encapsulation--fungicides--anti-fungal activity--average diameter--encapsulation efficiency--fusarium moniliforme--micro-particles--phytopathogenic fungi--poly-3-hydroxybutyrate--tebuconazole--fungi
Аннотация: In this study, tebuconazole (TEB)-loaded poly-3-hydroxybutyrate (P3HB)-based microparticles were developed and comprehensively characterized. TEB-loaded microparticles with the initial loading amounts of the fungicide of 10, 25, and 50% of the polymer mass (TEB 10, TEB 25, and TEB 50%) were prepared using emulsion technique. Encapsulation efficiency of TEB varied from 59 to 86%. As the loading amount was increased, the average diameter of microparticles increased too, from 41.3 to 71.7 µm, while zeta potential was not influenced by TEB loading, varying between –32.6 and –35.7 mV. TEB was gradually released from the microparticles to the model medium, and after 60 d, from 25 to 43% of TEB was released depending on the content of the encapsulated fungicide. The data obtained from in vitro TEB release were fitted to different mathematical models. It was shown that the release profiles of TEB could be best explained by the Zero-order, Higuchi, and Hixson–Crowell models. The antifungal activity of the P3HB/TEB microparticles against phytopathogenic fungi Fusarium moniliforme and Fusarium solani was demonstrated by in vitro tests conducted in Petri dishes. Thus, hydrophobic agrochemicals (TEB) can be effectively encapsulated into P3HB microparticles to construct slow-release formulations. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E. I., Menzyanova N. G., Shumilova A. A.
Заглавие : The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 141. - С. 765-774. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.09.015
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3?hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4?hydroxybutyrate (4HB), 3?hydroxyvalerate (3HV), or 3?hydroxyhexanoate (3HHx). All copolymers had the Mw of about 550–670 kDa, and the homopolymer P(3HB) had a significantly higher Mw – 920 kDa. P(3HB?co?4HB) and P(3HB?co?3HHx) had the lowest Cx (42 and 49%) while P(3HB?co?3HV) and P(3HB) exhibited higher Cx values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71–75 nm, than the copolymer films, which were hydrophilic (57–60°) and had considerably higher roughness (158–177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4?hydroxybutyrate and 3?hydroxyhexanoate were the best for contact with blood. © 2019 Elsevier B.V.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Zhila N., Shishatskaya E.
Заглавие : Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process
Место публикации : Biomacromolecules: American Chemical Society, 2019. - Article in press. - ISSN 15257797 (ISSN) , DOI 10.1021/acs.biomac.9b00295
Аннотация: The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture. © 2019 American Chemical Society.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Kazantseva E. A., Varygina D. E., Volova T. G.
Заглавие : Constructing Slow-Release Formulations of Ammonium Nitrate Fertilizer Based on Degradable Poly(3-hydroxybutyrate)
Место публикации : J. Agric. Food Chem.: American Chemical Society, 2017. - Vol. 65, Is. 32. - С. 6745-6752. - ISSN 00218561 (ISSN) , DOI 10.1021/acs.jafc.7b01217
Ключевые слова (''Своб.индексиров.''): ammonium nitrate--degradable poly-3-hydroxybutyrate--embedding--fillers--nitrogen fertilizers--tablets--chemical contamination--ecology--ecosystems--fertilizers--fillers--nitrates--plastic coatings--ammonium nitrate--ammonium nitrate fertilizers--embedding--in-laboratory experiments--poly-3-hydroxybutyrate--slow release fertilizers--tablets--wheat (triticum aestivum l.)--nitrogen fertilizers
Аннотация: The present study describes construction and investigation of experimental formulations of ammonium nitrate embedded in a matrix of degradable natural polymer poly-3-hydroxybutyrate [P(3HB)] and P(3HB) blended with wood flour shaped as tablets, some of them coated with P(3HB). Kinetics of ammonium release into soil as dependent on the composition of the polymer matrix was investigated in laboratory experiments. The rates of fertilizer release from formulations coated with a biopolymer layer were considerably (two months or longer) slower than the rates of fertilizer release from uncoated formulations, while release from polymer and composite (polymer/wood flour) formulations occurred with comparable rates. The use of the experimental formulations in laboratory ecosystems with wheat (Triticum aestivum L.) was more effective than application of free ammonium nitrate. The advantage of the slow-release fertilizer formulations is that they are buried in soil together with the seeds, and the fertilizer remains effective over the first three months of plant growth. The use of such slow-release formulations will reduce the amounts of chemicals released into the environment, which will curb their accumulation in food chains of ecosystems and mitigate their adverse effects on the biosphere. © 2017 American Chemical Society.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Zhila, Natalia, Vinogradova, Olga, Shumilova, Anna, Prudnikova, Svetlana, Shishatskaya, Ekaterina
Заглавие : Characterization of biodegradable poly-3-hydroxybutyrate films and pellets loaded with the fungicide tebuconazole
Колич.характеристики :12 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2016. - Vol. 23, Is. 6. - С. 5243-5254. - ISSN 0944-1344, DOI 10.1007/s11356-015-5739-1. - ISSN 1614-7499(eISSN)
Примечания : Cited References:33. - This study was supported by the Russian Science Foundation (grant no. 14-26-00039).
Предметные рубрики: CONTROLLED-RELEASE
MICROBIAL-DEGRADATION
BRASSICA-NAPUS
OILSEED RAPE
Ключевые слова (''Своб.индексиров.''): poly(3-hydroxybutyrate)--tebuconazole--slow-release formulations--controlled release--antifungal activity
Аннотация: Biodegradable polymer poly(3-hydroxybutyrate) (P3HB) has been used as a matrix to construct slow-release formulations of the fungicide tebuconazole (TEB). P3HB/TEB systems constructed as films and pellets have been studied using differential scanning calorimetry, X-ray structure analysis, and Fourier transform infrared spectroscopy. TEB release from the experimental formulations has been studied in aqueous and soil laboratory systems. In the soil with known composition of microbial community, polymer was degraded, and TEB release after 35 days reached 60 and 36 % from films and pellets, respectively. That was 1.23 and 1.8 times more than the amount released to the water after 60 days in a sterile aqueous system. Incubation of P3HB/TEB films and pellets in the soil stimulated development of P3HB-degrading microorganisms of the genera Pseudomonas, Stenotrophomonas, Variovorax, and Streptomyces. Experiments with phytopathogenic fungi F. moniliforme and F. solani showed that the experimental P3HB/TEB formulations had antifungal activity comparable with that of free TEB.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Syromotina D. S., Surmenev R. A., Surmeneva M. A., Boyandin A. N., Nikolaeva E. D., Prymak O., Epple M., Ulbricht M., Oehr C., Volova T. G.
Заглавие : Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma
Место публикации : Mater. Sci. Eng. C. - 2016. - Vol. 62. - С. 450-457. - ISSN 09284931 (ISSN) , DOI 10.1016/j.msec.2016.01.075
Ключевые слова (''Своб.индексиров.''): plasma--polar component--poly-3-hydroxybutyrate membranes--surface energy--surface treatment--ammonia--biodegradable polymers--cell adhesion--contact angle--free energy--interfacial energy--plasma applications--plasmas--surface chemistry--surface properties--surface roughness--surface topography--wetting--biological performance--crystalline structure--polar components--poly-3-hydroxybutyrate--surface free energy--surface roughness changes--surface wettability--water contact angle--surface treatment
Аннотация: The surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer, with the exception being a power level of 250 W. The wettability of the modified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. It was revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma. © 2016 Elsevier B.V.
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