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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Место публикации : Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - С. 719-728. - ISSN 09574530 (ISSN) , DOI 10.1023/B:JMSM.0000030215.49991.0d
Ключевые слова (''Своб.индексиров.''): necrosis--polymeric implants--suppurative inflammation--tissue reaction--biodegradation--calcification (biochemistry)--cells--copolymers--implants (surgical)--silk--tissue--tumors--materials science--acid phosphatase--copolymer--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polyhydroxyvaleric acid--unclassified drug--animal experiment--animal model--article--biodegradable implant--blood vessel reactivity--catgut--controlled study--enzyme activity--female--giant cell--histochemistry--inflammation--macrophage--nonhuman--priority journal--rat--silk--suture--tensile strength--tissue reaction--tissue structure--wound healing--young modulus--absorbable implants--animals--female--fibrosis--foreign-body reaction--hydroxybutyrates--muscle, skeletal--polyesters--polymers--rats--rats, wistar--sutures--treatment outcome--wounds, penetrating--animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Колич.характеристики :10 с
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 6. - P719-728. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000030215.49991.0d
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLYESTERS
POLYMERS
FIBERS
PHB
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types-polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV)-featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 mum thick, which became as thin as 40-60 mum after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner. (C) 2004 Kluwer Academic Publishers.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Efremova S.N., Puzyr S.P., Mogil'naya O.A.
Заглавие : Tissue response to biodegradable suture threads made of polyoxyalkanoates
Место публикации : Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 23-29. - ISSN 00258075 (ISSN)
Ключевые слова (''Своб.индексиров.''): biocompatibility--biodegradation--implants (surgical)--materials testing--organic polymers--polyoxyalkanoates--suture threads--biomaterials--biomaterial--polymer--animal--article--bioremediation--female--metabolism--rat--suture--wistar rat--animals--biocompatible materials--biodegradation, environmental--female--polymers--rats--rats, wistar--sutures
Аннотация: The paper presents data on the materials and methods of obtaining suture threads based on of the polymer batty acid derivatives (polyoxyalkanoates). Results of the study of tissue response to biodegradable polyoxyalkanoate suture threads in the postoperative period are analyzed. These suture threads have been shown to have the required strength and meet a number of other requirements for currently available materials.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Tissue reaction to intramuscular injection of resorbable polymer microparticles
Место публикации : Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - С. 786-790. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-007-0432-0
Ключевые слова (''Своб.индексиров.''): microencapsulation--polyhydroxybutyrate--resorbable polymers--tissue reaction--poly(3 hydroxybutyric acid)--polymer--animal experiment--animal tissue--article--cell infiltration--controlled study--drug delivery system--drug formulation--female--giant cell--inflammation--macrophage--microencapsulation--nonhuman--rat--tissue reaction--absorbable implants--animals--drug compounding--female--foreign-body reaction--injections, intramuscular--microspheres--polyesters--rats--rats, wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogil'naya O.A., Efremov S.N., Gitelson I.I.
Заглавие : Tissue morphogenesis under the conditions of implantation of polyhydroxybutyrate, a biodegradable polymer.
Место публикации : Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - С. 123-126. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): biomaterial--hydroxybutyric acid--polymer--animal--article--bioremediation--female--morphogenesis--rat--wistar rat--animals--biocompatible materials--biodegradation, environmental--female--hydroxybutyrates--morphogenesis--polymers--rats--rats, wistar
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Jose C., Chan C. H., Winie T., Joseph B., Tharayil A., Maria H. J., Volova T., Mantia F. P.L., Rouxel D., Morreale M., Laroze D., Mathew L., Thomas S.
Заглавие : Thermomechanical analysis of isora nanofibril incorporated polyethylene nanocomposites
Место публикации : Polym.: MDPI AG, 2021. - Vol. 13, Is. 2. - Ст.299. - С. 1-15. - ISSN 20734360 (ISSN), DOI 10.3390/polym13020299
Аннотация: The research on cellulose fiber-reinforced nanocomposites has increased by an unprecedented magnitude over the past few years due to its wide application range and low production cost. However, the incompatibility between cellulose and most thermoplastics has raised significant challenges in composite fabrication. This paper addresses the behavior of plasma-modified polyethylene (PE) reinforced with cellulose nanofibers extracted from isora plants (i.e., isora nanofibrils (INFs)). The crystallization kinetics of PE–INF composites were explained using the Avrami model. The effect of cellulose nanofillers on tuning the physiochemical properties of the nanocomposite was also explored in this work. The increase in mechanical properties was due to the uniform dispersion of fillers in the PE. The investigation on viscoelastic properties confirmed good filler–matrix interactions, facilitating the stress transfer. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Jose, Cintil, Chan, Chin Han, Winie, Tan, Joseph, Blessy, Tharayil, Abhimanyu, Maria, Hanna J., Volova, Tatiana, La Mantia, Francesco Paolo, Rouxel, Didier, Morreale, Marco, Laroze, David, Mathew, Lovely, Thomas, Sabu
Заглавие : Thermomechanical Analysis of Isora Nanofibril Incorporated Polyethylene Nanocomposites
Колич.характеристики :15 с
Коллективы : project "Agro preparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]
Место публикации : Polymers: MDPI, 2021. - Vol. 13, Is. 2. - Ст.299. - ISSN 2073-4360(eISSN), DOI 10.3390/polym13020299
Примечания : Cited References:46. - This study (polymer synthesis and investigation) was financially supported by the project "Agro preparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation on 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".
Аннотация: The research on cellulose fiber-reinforced nanocomposites has increased by an unprecedented magnitude over the past few years due to its wide application range and low production cost. However, the incompatibility between cellulose and most thermoplastics has raised significant challenges in composite fabrication. This paper addresses the behavior of plasma-modified polyethylene (PE) reinforced with cellulose nanofibers extracted from isora plants (i.e., isora nanofibrils (INFs)). The crystallization kinetics of PE-INF composites were explained using the Avrami model. The effect of cellulose nanofillers on tuning the physiochemical properties of the nanocomposite was also explored in this work. The increase in mechanical properties was due to the uniform dispersion of fillers in the PE. The investigation on viscoelastic properties confirmed good filler-matrix interactions, facilitating the stress transfer.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Thomas S., Shumilova A. A., Kiselev E. G., Baranovsky S. V., Vasiliev A. D., Nemtsev I. V., Kuzmin A. P., Sukovatyi A. G., Avinash R. P., Volova T. G.
Заглавие : Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Article in press. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.11.112
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour – were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P(3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P(3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. © 2018
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Thomas, Sabu, Shumilova A. A., Kiselev E. G., Baranovsky, S., V, Vasiliev A. D., Nemtsev, I., V, Kuzmin, Andrei Petrovich, Sukovatyi A. G., Avinash, R. Pai, Volova T. G.
Заглавие : Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites
Колич.характеристики :12 с
Коллективы : Government of the Russian Federation [220]; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER, 2020. - Vol. 155. - С. 1373-1384. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2019.11.112. - ISSN 1879-0003(eISSN)
Примечания : Cited References:38. - This studywas financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".; The surface of the samples was investigated using a scanning electron microscope Hitachi TM-3000 in the Joint Instrument Use Center at the Krasnoyarsk Scientific Center of Siberian Branch of Russian Academy of Sciences.
Предметные рубрики: FORMULATIONS
POLYHYDROXYALKANOATES
POLYHYDROXYBUTYRATE
SOIL
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour - were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P (3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P (3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. (C) 2019 Published by Elsevier B.V.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belyaev B.A., Volova T.G., Drokin N.A., Shepov V.N.
Заглавие : The SHF dielectric permeability of polyhydroxybutyrate, a degradable biological polymer.
Место публикации : Doklady Biophysics. - 2000. - Vol. 370-372. - С. 9-12. - ISSN 00124974 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydroxybutyric acid--polymer--article--chemistry--electricity--electromagnetic field--temperature--electromagnetic fields--electrostatics--hydroxybutyrates--polymers--temperature
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Zhila N.O., Shishatskaya E.I., Mironov P.V., Vasil'Ev A.D., Sukovatyi A.G., Sinskey A.J.
Заглавие : The physicochemical properties of polyhydroxyalkanoates with different chemical structures
Место публикации : Polymer Science - Series A. - 2013. - Vol. 55, Is. 7. - С. 427-437. - ISSN 0965545X (ISSN) , DOI 10.1134/S0965545X13070080
Ключевые слова (''Своб.индексиров.''): amorphous and crystalline phasis--degrees of crystallinity--physicochemical property--poly(3-hydroxybutirate)--polyhydroxyalkanoate copolymer--temperature characteristic--thermal degradation temperatures--thermoplastic materials--copolymers--monomers--thermoplastic elastomers--structure (composition)
Аннотация: A set of polyhydroxyalkanoates are synthesized, and a comparative study of their physicochemical properties is performed. The molecular masses and polydispersities of polyhydroxyalkanoates are found to be independent of their chemical structures. It is shown that the temperature characteristics and degrees of crystallinity of polyhydroxyalkanoates are affected by the chemical compositions of the monomers and their quantitative contents in the polymers. The incorporation of 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate units into the chain of poly(3-hydroxybutyrate) decreases its melting point and thermal degradation temperature relative to these parameters of a homogeneous poly(3-hydroxybutyrate) sample (175 В± 5 C and 275 В± 5 C, respectively). The higher the content of the second monomer units in the poly(3-hydroxybutirate) chain, the greater the changes. The degrees of crystallinity of polyhydroxyalkanoate copolymers are generally lower than that of poly(3-hydroxybutyrate) (75 В± 5%). The effect on the ratio of the amorphous and crystalline phases of the copolymer samples becomes more pronounced in the series 3-hydroxy-valerate-3-hydroxyhexanoate-4- hydroxybutyrate. The prepared samples exhibit different properties ranging from rigid thermoplastic materials to engineering elastomers. В© 2013 Pleiades Publishing, Ltd.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S., Streltsova N., Volova T.
Заглавие : The effect of the pesticide delivery method on the microbial community of field soil
Место публикации : Environ. Sci. Pollut. Res.: Springer Science and Business Media Deutschland GmbH, 2020. - Article in press. - ISSN 09441344 (ISSN), DOI 10.1007/s11356-020-11228-7
Аннотация: The study deals with the effects of herbicides (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl) and fungicides (tebuconazole, epoxiconazole, azoxystrobin) applied to soil as free pesticides or as slow release formulations embedded in a biodegradable composite matrix on the structure of the soil microbial community. The matrix consisted of a natural biopolymer poly-3-hydroxybutyrate [P(3HB)] and a filler—one of the natural materials (peat, clay, and wood flour). The soil microbial community was characterized, including the major eco-trophic groups of bacteria, dominant taxa of bacteria and fungi, and primary P(3HB)-degrading microorganisms, such as Pseudomonas, Bacillus, Pseudarthrobacter, Streptomyces, Penicillium, and Talaromyces. The addition of free pesticides adversely affected the abundance of soil microorganisms; the decrease varied from 1.4 to 56.0 times for different types of pesticides. The slow release pesticide formulations, in contrast to the free pesticides, exerted a much weaker effect on soil microorganisms, no significant inhibition in the abundance of saprotrophic bacteria was observed, partly due to the positive effects of the composite matrix (polymer/natural material), which was a supplementary substrate for microorganisms. The slow release fungicide formulations, like the free fungicides, reduced the total abundance of fungi and inhibited the development of the phytopathogens Fusarium and Alternaria. Thus, slow release formulations of pesticides preserve the bioremediation potential of soil microorganisms, which are the main factor of removing xenobiotics from the biosphere. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova, Svetlana, Streltsova, Nadezhda, Volova, Tatiana
Заглавие : The effect of the pesticide delivery method on the microbial community of field soil
Колич.характеристики :17 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation, project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2020. - Article in press. - ISSN 0944-1344, DOI 10.1007/s11356-020-11228-7. - ISSN 1614-7499(eISSN)
Примечания : Cited References:119. - This study was financially supported by the Ministry of Science and Higher Education of the Russian Federation, project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328).
Предметные рубрики: CONTROLLED-RELEASE
2,4-DICHLOROPHENOXYACETIC ACID
DEGRADATION
Аннотация: The study deals with the effects of herbicides (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl) and fungicides (tebuconazole, epoxiconazole, azoxystrobin) applied to soil as free pesticides or as slow release formulations embedded in a biodegradable composite matrix on the structure of the soil microbial community. The matrix consisted of a natural biopolymer poly-3-hydroxybutyrate [P(3HB)] and a filler-one of the natural materials (peat, clay, and wood flour). The soil microbial community was characterized, including the major eco-trophic groups of bacteria, dominant taxa of bacteria and fungi, and primary P(3HB)-degrading microorganisms, such asPseudomonas,Bacillus,Pseudarthrobacter,Streptomyces,Penicillium, andTalaromyces. The addition of free pesticides adversely affected the abundance of soil microorganisms; the decrease varied from 1.4 to 56.0 times for different types of pesticides. The slow release pesticide formulations, in contrast to the free pesticides, exerted a much weaker effect on soil microorganisms, no significant inhibition in the abundance of saprotrophic bacteria was observed, partly due to the positive effects of the composite matrix (polymer/natural material), which was a supplementary substrate for microorganisms. The slow release fungicide formulations, like the free fungicides, reduced the total abundance of fungi and inhibited the development of the phytopathogensFusariumandAlternaria. Thus, slow release formulations of pesticides preserve the bioremediation potential of soil microorganisms, which are the main factor of removing xenobiotics from the biosphere.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E. I., Menzyanova N. G., Shumilova A. A.
Заглавие : The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 141. - С. 765-774. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.09.015
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3?hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4?hydroxybutyrate (4HB), 3?hydroxyvalerate (3HV), or 3?hydroxyhexanoate (3HHx). All copolymers had the Mw of about 550–670 kDa, and the homopolymer P(3HB) had a significantly higher Mw – 920 kDa. P(3HB?co?4HB) and P(3HB?co?3HHx) had the lowest Cx (42 and 49%) while P(3HB?co?3HV) and P(3HB) exhibited higher Cx values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71–75 nm, than the copolymer films, which were hydrophilic (57–60°) and had considerably higher roughness (158–177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4?hydroxybutyrate and 3?hydroxyhexanoate were the best for contact with blood. © 2019 Elsevier B.V.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya, Ekaterina I., Menzyanova, Natalia G., Shumilova, Anna A.
Заглавие : The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response
Колич.характеристики :11 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [17-15-01352]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER, 2019. - Vol. 141. - С. 765-+. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2019.09.015. - ISSN 1879-0003(eISSN)
Примечания : Cited References:57. - The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The study is supporting by the Russian Science Foundation, Project No 17-15-01352.
Предметные рубрики: 3-HYDROXYBUTYRATE
BIOCOMPATIBILITY
BIOSYNTHESIS
ADHESION
STENTS
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3 hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4 hydroxybutyrate (4HB), 3 hydroxyvalerate (3HV), or 3 hydroxyhexanoate (3HHx). All copolymers had the M-w of about 550-670 kDa, and the homopolymer P(3HB) had a significantly higher M-w - 920 kDa. P(3HB co 4HB) and P(3HB co 3HHx) had the lowest C-x (42 and 49%) while P(3HB co 3HV) and P(3HB) exhibited higher C-x values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71-75 nm, than the copolymer films, which were hydrophilic (57-60 degrees) and had considerably higher roughness (158-177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4 hydroxybutyrate and 3 hydroxyhexanoate were the best for contact with blood. (C) 2019 Elsevier B.V. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Kalacheva G.S., Rodicheva E.K., Volova T.G.
Заглавие : Synthesis of reserve polyhydroxyalkanoates by luminescent bacteria
Место публикации : Microbiology. - 2008. - Vol. 77, Is. 3. - С. 318-323. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261708030119
Ключевые слова (''Своб.индексиров.''): biosynthesis--chemical structure--luminescent bacteria--polyhydroxyalkanoates (pha)--bacteria (microorganisms)--photobacterium leiognathi--vibrio harveyi
Аннотация: The ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, PHA) as reserve macromolecules was studied. Twenty strains from the collection of the luminescent bacteria CCIBSO (WDCM839) of the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, assigned to different taxa (Photobacterium leiognathi, Ph. phosphoreum, Vibrio harveyi, and V. fischeri) were analyzed. The most productive strains were identified, and the conditions ensuring high polymer yields in batch culture (40-70% of the cell dry mass weight) were determined. The capacity for synthesizing two-and three-component polymers containing hydroxybutyric acid as the main monomer and hydroxyvaleric and hydroxyhexanoic acids was revealed in Ph. leiognathi and V. harveyi strains. The results allow luminescent microorganisms to be regarded as new producers of multicomponent polyhydroxyalkanoates. В© 2008 MAIK Nauka.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Zhila N., Shishatskaya E.
Заглавие : Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process
Место публикации : Biomacromolecules: American Chemical Society, 2019. - Article in press. - ISSN 15257797 (ISSN) , DOI 10.1021/acs.biomac.9b00295
Аннотация: The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture. © 2019 American Chemical Society.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Zhila, Natalia, Shishatskaya, Ekaterina
Заглавие : Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium Seliberia carboxydohydrogena Z-1062
Колич.характеристики :11 с
Коллективы : Russian Academy of Sciences [01201351505]
Место публикации : J. Ind. Microbiol. Biotechnol.: SPRINGER HEIDELBERG, 2015. - Vol. 42, Is. 10. - С. 1377-1387. - ISSN 1367-5435, DOI 10.1007/s10295-015-1659-9. - ISSN 1476-5535(eISSN)
Примечания : Cited References:36. - The research was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505).
Предметные рубрики: CARBON-MONOXIDE
RALSTONIA-EUTROPHA
POLYHYDROXYALKANOATES
GROWTH
H-2
Ключевые слова (''Своб.индексиров.''): carboxydobacteria--synthesis--poly(3-hydroxybutyrate)--carbon monoxide
Аннотация: The present study addresses growth parameters and physiological and biochemical characteristics of the aerobic CO-oxidizing carboxydobacterium Seliberia carboxydohydrogena Z-1062. Poly(3-hydroxybutyrate) yields were investigated in experiments with limiting concentrations of mineral nutrients (nitrogen or sulfur or nitrogen and sulfur) in batch culture of S. carboxydohydrogena Z-1062 grown on gas mixtures consisting of CO2, O-2, H-2, and CO. CO concentrations of 10, 20, and 30 % v/v did not affect polymer synthesis, whose content after 56-h cultivation under limiting concentrations of nitrogen and sulfur was 52.6-62.8 % of biomass weight at a productivity of 0.13-0.22 g/L h. The inhibitory effect of CO on cell concentration was revealed at CO concentration of 30 % v/v. That also caused a decrease in substrate (H-2 and O-2) use efficiency. Thus, this carboxydobacterium can be regarded as a potential producer of polyhydroxyalkanoates from industrial hydrogenous sources.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Syrvacheva D. A., Zhila N. O., Sukovatiy A. G.
Заглавие : Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoate monomer by Cupriavidus eutrophus B10646
Место публикации : J. Chem. Technol. Biotechnol. - 2016. - Vol. 91, Is. 2. - С. 416-425. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.4592
Ключевые слова (''Своб.индексиров.''): growth kinetics--physicochemical and mechanical properties--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain cupriavidus eutrophus b10646--biomaterials--biomechanics--chemical industry--cultivation--growth kinetics--mechanical properties--organic compounds--polymers--sodium--3-hydroxyhexanoate--bacterial strains--cultivation conditions--kinetic properties--physico-chemical and mechanical properties--physiological range--poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)--wild-type strain--strain
Аннотация: BACKGROUND: P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS: Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION: For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5-6gL-1) and high content of the polymer (60-75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. © 2014 Society of Chemical Industry © 2016 Society of Chemical Industry.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Zhila N.O., Kalacheva G.S., Sokolenko V.A., Sinski E.J.
Заглавие : Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria
Место публикации : Applied Biochemistry and Microbiology. - 2011. - Vol. 47, Is. 5. - С. 494-499. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683811050152
Ключевые слова (''Своб.индексиров.''): bacteria (microorganisms)--cupriavidus--cupriavidus necator
Аннотация: Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-co-4HB), the most promising member of the biodegradable polyhydroxyalkanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-co-4HB copolymer. A set of highly pure samples of the 3HB-co-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol% has been obtained. Incorporation of 4HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-co-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa). В© 2010 Pleiades Publishing, Ltd.
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