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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T. V., Shikhov V. N., Tikhomirov A. A.
Заглавие : The fluorescence method for determining of photosynthetic apparatus reactivity in plant leaves
Колич.характеристики :13 с
Место публикации : Zhurnal Obshchei Biol.: MEZHDUNARODNAYA KNIGA, 2019. - Vol. 80, Is. 3. - С. 187-199. - ISSN 0044-4596, DOI 10.1134/S0044459619030060
Примечания : Cited References:31
Предметные рубрики: ELECTRIC-POTENTIAL DIFFERENCE
FREQUENTLY ASKED QUESTIONS
CHLOROPHYLL
Аннотация: Presently, the most promising way of studying, forecasting, and enhancing of organisms' tolerance to harsh environmental impacts is considered to be the estimation of initial functional state of an organism's regulatory systems. To resolve the problem of photosynthetic apparatus (PSA) tolerance to harsh impacts at the level of such a complicated functional system as a plant leaf, it is necessary to assess integral responses of the leaf's PSA to the impact. At that, simple and versatile traits may have certain advantages. At present, chlorophyll fluorescence seems to be one of the main indices of PSA activity, which can be measured relatively fast and easy. One of the possible approaches to operational integrative assessment of PSA activity may consist in usage of the parameters introduced for the curves of chlorophyll fluorescence induction (CFI) slow phase. Temporal patterns of CFI are of special interest. The simplest index T-0.5 (i.e., half-time of chlorophyll fluorescence intensity decrease during the slow phase of CFI) appears to be an integral characteristic of activation rate with regard to a number of photo-assimilation and photo-protective processes in leaves. On basis of the studies, conducted earlier, and published data, we have analyzed the behavior of T-0.5 parameter with comparison to other CFI traits (namely: qN - non-photochemical quenching coefficient, ETR - electron transport rate, Phi(PSII) - effective quantum yield of PSII photochemistry, F-p/F-T ratio) under different conditions. The influence of leaf senescence, changes in intensity of excitation light, slight dehydration of plants and their recovery from water deficiency have been examined. The pattern of T-0.5 behavior, observed in laboratory experiments, and the results of its comparison with other indices of CFI give occasion to propose the usage of T-0.5 for indirect estimation of PSA activity when operational integrative monitoring of PSA state is required. Further studies are necessary for establishing quantitative relationships between PSA activity and fluorescence parameter T-0.5 under specific stress conditions.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Baranovsky, Sergey, Petrovskaya, Olga, Shumilova, Anna, Sukovatyi, Alexey
Заглавие : Biological effects of the free and embedded metribuzin and tribenuron-methyl herbicides on various cultivated weed species
Колич.характеристики :11 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" [220]
Место публикации : J. Environ. Sci. Health Part B-Pestic. Contam. Agric. Wastes: TAYLOR & FRANCIS INC, 2020. - ISSN 0360-1234, DOI 10.1080/03601234.2020.1807835. - ISSN 1532-4109(eISSN)
Примечания : Cited References:42. - This work was supported by Project "Agropreparations of the new generation: a strategy of construction and realization" [Agreement No 074-02-2018-328] in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".
Предметные рубрики: SYNTHASE-INHIBITING HERBICIDES
CHLOROPHYLL FLUORESCENCE
RELEASE
Аннотация: The present study addresses the herbicidal activity and biological effects of the metribuzin (MET) and tribenuron-methyl (TBM) herbicides used to control various weed species (Amaranthus retroflexus, Sinapis arvensis,andLeucanthemum maximum). The effects of the free herbicides and the herbicides embedded in granules of degradable polymer poly-3-hydroxybutyrate [P(3HB)] blended with birch wood flour were compared. Metribuzin, regardless of the form, caused 100% mortality of the three weeds by day 21. The herbicidal activity of tribenuron-methyl was lower than that of metribuzin, but the embedded TBM was superior to the free herbicide in the length and strength of its action on the weeds. Both metribuzin forms dramatically decreased the main parameters of fluorescence: maximum quantum yield of photosystem-II [Y(II)(max)], maximum quantum yield of non-photochemical quenching [Y(NPQ)(max)], and maximum rate of non-cyclic electron transport [ETRmax] and concentrations of chlorophyllaandb. The effect of the embedded TBM on the photosynthetic activity of the weeds was lower in the first two weeks of the growth of herbicide-treated plants but lasted longer than the effect of the free TBM and increased over time. Embedding of metribuzin in the matrix of degradable blend did not decrease its herbicidal activity.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T. V., Shikhov V. N., Tikhomirov A. A.
Заглавие : Effect of light intensity on the age dependence of nonphotochemical fluorescence quenching in wheat leaf
Колич.характеристики :4 с
Коллективы : RAS [56.1.4]
Место публикации : Photosynthetica: SPRINGER, 2015. - Vol. 53, Is. 4. - С. 617-620. - ISSN 0300-3604, DOI 10.1007/s11099-015-0133-2. - ISSN 1573-9058(eISSN)
Примечания : Cited References:16. - The study was performed within the framework of subject No. 56.1.4., Section VI in accordance with State Program of RAS Fundamental Research for 2013-2020.
Предметные рубрики: CHLOROPHYLL FLUORESCENCE
PHOTOSYNTHESIS
LEAVES
INDUCTION
ENERGY
Ключевые слова (''Своб.индексиров.''): chlorophyll fluorescence induction--leaf age--photosystem ii
Аннотация: The effects of actinic light (AL) intensity on the age dependence of nonphotochemical fluorescence quenching (q(N)) and effective quantum yield in PSII (I broken vertical bar(PSII)) were studied in continuously illuminated wheat leaves of the upper tier. Regular changes were revealed in both age dependence of q(N) at elevated AL intensities and light curves of q(N). These changes are related to alterations in strategies of redistribution and use of absorbed light energy by the photosynthetic apparatus at different stages of wheat leaf development. Unlike I broken vertical bar(PSII), q(N) as a parameter was more sensitive to the differences in the leaf age at a certain range of light intensities. At the same time, the stability of q(N) at moderate light intensities may serve as an indication of leaf maturity.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T. V., Shikhov V. N., Tikhomirov A. A.
Заглавие : Estimation of changes in the activity of photosynthetic apparatus of plant leaves based on half-time of fluorescence intensity decrease
Колич.характеристики :5 с
Место публикации : Photosynthetica: SPRINGER, 2019. - Vol. 57, Is. 1. - С. 132-136. - ISSN 0300-3604, DOI 10.32615/ps.2019.005. - ISSN 1573-9058(eISSN)
Примечания : Cited References:34
Предметные рубрики: ELECTRIC-POTENTIAL DIFFERENCE
CHLOROPHYLL FLUORESCENCE
THYLAKOID
Аннотация: The range of variations in parameter tau(0.5) - half-time of fluorescence intensity decrease during the slow phase of chlorophyll fluorescence induction (CFI) - has been studied during ontogeny of leaves of monocotyledonous and dicotyledonous plants in plant communities of different structures. Plants were grown hydroponically on expanded clay aggregate in growth chambers, under PPFD of 400 mu mol(photon) m(-2) s(-1), under controlled conditions. Analysis of the literature data and results of experimental observations of tau(0.5) behavior compared to other CFI parameters, nonphotochemical quenching coefficient, q(N), in particular, leads to the conclusion that parameter tau(0.5) can be effectively used for indirect estimation of variations in the activity of photosynthetic apparatus during ontogeny of plant leaves.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nemtseva, Elena, V, Gulnov, Dmitry, V, Gerasimova, Marina A., Sukovatyi, Lev A., Burakova, Ludmila P., Karuzina, Natalya E., Melnik, Bogdan S., Kratasyuk, Valentina A.
Заглавие : Bacterial Luciferases from Vibrio harveyi and Photobacterium leiognathi Demonstrate Different Conformational Stability as Detected by Time-Resolved Fluorescence Spectroscopy
Колич.характеристики :17 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; RFBRRussian Foundation for Basic Research (RFBR) [20-34-90118]; Krasnoyarsk Regional Fund of Science [20-44-243002, 20-44-240006]; RFBRRussian Foundation for Basic Research (RFBR)
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 19. - Ст.10449. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms221910449
Примечания : Cited References:45. - The research was partially funded by the Ministry of Science and Higher Education of the Russian Federation (projects No. FSRZ-2020-0006); by the RFBR and Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (projects No. 20-44-243002 and 20-44-240006); and by the RFBR (project No. 20-34-90118).
Предметные рубрики: TRYPTOPHAN FLUORESCENCE
CRYSTAL-STRUCTURE
SUBUNIT
BIOLUMINESCENCE
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of alpha-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods./p
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pavlova A. M., Gaevskii N. A., Anishchenko O. V., Tikhomirova N. A., Tikhomirov A. A.
Заглавие : Influence of NaCl on Productivity and Fluorescence Parameters of Nasturtium officinale R. Br. and Its Relevance to Artificial Closed Ecosystems
Колич.характеристики :13 с
Коллективы : fundamental research program of the Russian Academy of SciencesRussian Academy of Sciences [56.1.4]
Место публикации : Russ. J. Plant Physiol.: PLEIADES PUBLISHING INC, 2021. - Vol. 68, Is. 6. - С. 1173-1185. - ISSN 1021-4437, DOI 10.1134/S1021443721050137. - ISSN 1608-3407(eISSN)
Примечания : Cited References:27. - This work was supported by the fundamental research program of the Russian Academy of Sciences for 2013-2020, project no. 56.1.4 Sustainability of Higher Plant Cenoses Grown on Nutrient Media with Mineralized Organic Waste in Closed Human-Inhabited Ecological Systems.
Предметные рубрики: CHLOROPHYLL FLUORESCENCE
SALT STRESS
TOLERANCE
PHOTOSYNTHESIS
Аннотация: Productivity values, sodium accumulation in aboveground biomass, and photosynthetic indices of watercress (Nasturtium officinale) leaves were investigated under conditions resembling artificial closed ecological systems (CES). The seedlings were grown on nutrient media with various NaCl concentrations (0.7, 1.4, and 1.8 g/L) for 7, 14, and 19 days after transferring them to saline solutions. The productivity of plants on the seventh day of their growth on saline media did not differ from that of control plants. The decrease in plant productivity was noted in all the treatments starting from the 14th day after transferring the plants to saline solutions. When NaCl concentration in the nutrient solution was raised from 0.7 to 1.8 g/L, a significant increase in relative Na+ content in plant tissues was observed, regardless of the duration of NaCl treatment. A substantial decrease in chlorophyll (a + b) to carotenoid content ratio was noted on the seventh and 14th days in plants grown at 1.8 g/L NaCl. In plants treated for 7 days with 0.7 and 1.4 g/L NaCl, the content of chlorophylls a and b and carotenoids was found to increase, which indicates the tolerance of N. officinale to CES conditions. The relative content of chlorophylls a and b in the light-harvesting chlorophyll (a + b) complex was independent of the extent of salinity. The maximum quantum yield of photosystem II reaction in N. officinale plants had typically high values (Y(II)(max) of 0.755 +/- 0.007). Using the Imaging Maxi version of the pulse amplitude-modulated (PAM) fluorometer, it was found that light curves for the effective quantum yield of photochemical and nonphotochemical fluorescence quenching (Y(II) and Y(NPQ), respectively) differed appreciably between the salt-treated and untreated plants in the case of long-term cultivation (19 days) at 0.7 and 1.4 g/L NaCl. The treatment with 1.8 g/L NaCl for the period from 14 to 19 days had no effect on light curves of Y(II) and Y(NPQ). It is argued that N. officinale can be used as a source of NaCl for humans under CES conditions.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Baranovsky S., Petrovskaya O., Shumilova A., Sukovatyi A.
Заглавие : Biological effects of the free and embedded metribuzin and tribenuron-methyl herbicides on various cultivated weed species
Место публикации : J. Environ. Sci. Health Part B Pestic. Food Contamin. Agric. Wastes: Taylor and Francis Inc., 2020. - Article in press. - ISSN 03601234 (ISSN), DOI 10.1080/03601234.2020.1807835
Аннотация: The present study addresses the herbicidal activity and biological effects of the metribuzin (MET) and tribenuron-methyl (TBM) herbicides used to control various weed species (Amaranthus retroflexus, Sinapis arvensis, and Leucanthemum maximum). The effects of the free herbicides and the herbicides embedded in granules of degradable polymer poly-3-hydroxybutyrate [P(3HB)] blended with birch wood flour were compared. Metribuzin, regardless of the form, caused 100% mortality of the three weeds by day 21. The herbicidal activity of tribenuron-methyl was lower than that of metribuzin, but the embedded TBM was superior to the free herbicide in the length and strength of its action on the weeds. Both metribuzin forms dramatically decreased the main parameters of fluorescence: maximum quantum yield of photosystem-II [Y(II)max], maximum quantum yield of non-photochemical quenching [Y(NPQ)max], and maximum rate of non-cyclic electron transport [ETRmax] and concentrations of chlorophyll a and b. The effect of the embedded TBM on the photosynthetic activity of the weeds was lower in the first two weeks of the growth of herbicide-treated plants but lasted longer than the effect of the free TBM and increased over time. Embedding of metribuzin in the matrix of degradable blend did not decrease its herbicidal activity. © 2020 Taylor & Francis Group, LLC.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nemtseva E. V., Gulnov D. V., Gerasimova M. A., Sukovatyi L. A., Burakova L. P., Karuzina N. E., Melnik B. S., Kratasyuk V. A.
Заглавие : Bacterial luciferases from vibrio harveyi and photobacterium leiognathi demonstrate different conformational stability as detected by time-resolved fluorescence spectroscopy
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 19. - Ст.10449. - ISSN 16616596 (ISSN), DOI 10.3390/ijms221910449
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of ?-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Shalayeva Ye.V., Zadorozhnaya Ye.N., Kratasyuk V.A., Balayan A.E., Stom D.I.
Заглавие : Patterns of inhibition of bacterial bioluminescence in vitro by quinones and phenols - Components of sewage
Место публикации : Biophysics. - 1994. - Vol. 39, Is. 3. - P441-451. - ISSN 00063509 (ISSN)
Аннотация: Quenching of bioluminescence of the bienzyme system bacterial luciferase-NAD В· H:FMN-oxidoreductaseвЂ вЂ NADВ·H is reduced nicotinamide adenine dinucleotide and FMN is flavine manonucleotide. is considered in terms of the patterns of intermolecular transfer of an electron and hydrogen between donor (bioluminescent centre) and acceptor (compound to be analysed) taking into account the structure of the acceptor molecule. The link between the induction period of bioluminescence and the redox potential of the quinone-acceptor has been confirmed. It is shown that the induction period and the time of passage to the bioluminescence maximum in the presence of quinones are determined by the size of the aromatic and aliphatic fragments of the quinone molecule; the intensity of bioluminescence depends on the efficiency of the processes of intermolecular electron transfer (donor-acceptor) and the competition of the compound being analysed with aldehyde for the binding site on luciferase. В© 1994.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Meshalkin Y.P., Nemtseva E.V., Alfimov E.E., Kudryasheva N.S.
Заглавие : On the quenching of bacterial luminescence by dyes
Колич.характеристики :5 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 1999. - Vol. 44, Is. 6. - P1083-1087. - ISSN 0006-3029
Примечания : Cited References: 5
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--dyes--quenching
Аннотация: It was shown that the addition of dyes (sodium fluorescein, rhodamine 6G, unsubstituted rhodamine) to a bienzymic reaction mixture. (luciferin-luciferase. complex): leads to a decrease: in fluorescence intensity and the appearance of the dye fluorescence band. A similar effect was observed when the luciferin-luciferase complex and dye molecules were separated by distances considerably. exceeding the Forster radius of transfer. It is assumed that the mechanism of dye. fluorescence is not related to the excitation energy resonance transfer but is based on the excitation of dye molecules due to direct absorption of quanta of bacterial bioluminescence.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kuznetsov A.M., Rodicheva E.K., Medvedeva S.E.
Заглавие : Biotesting of Effluent and River Water by Lyophilized Luminous Bacteria Biotest
Место публикации : Field Analytical Chemistry and Technology. - 1998. - Vol. 2, Is. 5. - С. 267-275. - ISSN 1086900X (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--effluent--luminous bacteria biotest--toxicity--water quality
Аннотация: Waters of the Yenisei River, certain rivers and lakes of the Altai Territory, and effluents of some industrial factories in Krasnoyarsk were studied by luminous bacteria biotest Microbiosensor B17 677F. The lyophilized luminous bacteria Photobacterium phosphoreum from the IBSO collection were used to design this biotest. The bioluminescent test is based on bioluminescence quenching resulting from the action of water samples on luminous bacteria. The test results indicated locations and zones of impaired water quality. The heaviest pollution of water in the Yenisei River was recorded in the zones 0-116 km downstream from Krasnoyarsk (Krasnoyarsk and satellite towns). The effluents of most factories were found to be toxic. Underground and surface waters of some areas of the Altai Territory had different toxicity levels; there were deviations from the norm in most water samples taken from the different lakes and rivers. The data from this study show that the luminous bacteria biotest is simple and convenient, and that the results obtained are within acceptable levels of accuracy for the evaluation of the toxicity of effluent and river water. It takes no more than 30 min to do the biotest. It can be used in ecological monitoring like the Microtox toxicity test. В© 1998 John Wiley & Sons, Inc.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Westphal A.H., Visser AJWG, van Berkel WJH, Vysotski E.S.
Заглавие : The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding
Колич.характеристики :6 с
Коллективы : Wageningen University Sandwich PhD-Fellowship Program [02.512.12. 2006]; Ministry of Education and Science of Russian Federation, MCB Program of RAS [1211.2008.4]; SB RAS
Место публикации : FEBS Lett.: ELSEVIER SCIENCE BV, 2009. - Vol. 583, Is. 12. - С. 1939-1944. - ISSN 0014-5793, DOI 10.1016/j.febslet.2009.04.043
Примечания : Cited References: 28. - We thank Prof. John Lee for valuable suggestions and providing constructive criticisms. The work was supported by Wageningen University Sandwich PhD-Fellowship Program, Grants 02.512.12. 2006 and 1211.2008.4 of Ministry of Education and Science of Russian Federation, MCB Program of RAS, and by Grant No. 2 of SB RAS.
Предметные рубрики: CRYSTAL-STRUCTURE
CA2+-REGULATED PHOTOPROTEINS
VIOLET BIOLUMINESCENCE
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
W92F OBELIN
CALCIUM
REGENERATION
APOAEQUORIN
EXPRESSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--photoprotein--trp fluorescence
Аннотация: The intrinsic fluorescence of two apo-photoproteins has been characterized and its concentration-dependent quenching by coelenterazine has been for the first time applied to determine the apparent dissociation constants for coelenterazine binding with apo-aequorin (1.2 +/- 0.12 mu M) and apo-obelin (0.2 +/- 0.04 mu M). Stopped-flow measurements of fluorescence quenching showed that coelenterazine binding is a millisecond-scale process, in contrast to the formation of an active photoprotein complex taking several hours. This finding evidently shows that the rate-limiting step of active photoprotein formation is the conversion of coelenterazine into its 2-hydroperoxy derivative. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Fedoseeva, Yu. V., Bulusheva L.G., Okotrub A.V., Kanygin M.A., Gorodetskiy D.V., Asanov I.P., Vyalikh D.V., Puzyr A.P., Bondar V.S.
Заглавие : Field emission luminescence of nanodiamonds deposited on the aligned carbon nanotube array
Колич.характеристики :7 с
Коллективы : RFBR grant [13-03-12118], bilateral Program "Russian-German Laboratory at BESSY''
Место публикации : Sci Rep: NATURE PUBLISHING GROUP, 2015. - Vol. 5. - Ст.9379. - ISSN 2045-2322, DOI 10.1038/srep09379
Примечания : Cited References:49. - The work was supported by RFBR grant 13-03-12118 in the part of electroluminescence measurements and the bilateral Program "Russian-German Laboratory at BESSY''. We are grateful to Mr. A.V. Ischenko for the TEM measurements, Mr. S.I. Kozhemyachenko for the Raman spectra, Mrs. N.I. Alferova for the IR spectra, and Mr. D.V. Gulyaev for the photoluminescence spectra.
Предметные рубрики: DETONATION NANODIAMOND
ULTRANANOCRYSTALLINE DIAMOND
Аннотация: Detonation nanodiamonds (NDs) were deposited on the surface of aligned carbon nanotubes (CNTs) by immersing a CNT array in an aqueous suspension of NDs in dimethylsulfoxide (DMSO). The structure and electronic state of the obtained CNT-ND hybrid material were studied using optical and electron microscopy and Infrared, Raman, X-ray photoelectron and near-edge X-ray absorption fine structure spectroscopy. A non-covalent interaction between NDs and CNT and preservation of vertical orientation of CNTs in the hybrid were revealed. We showed that current-voltage characteristics of the CNT-ND cathode are changed depending on the applied field; below similar to 3 V/mu m they are similar to those of the initial CNT array and at the higher field they are close to the ND behavior. Involvement of the NDs in field emission process resulted in blue luminescence of the hybrid surface at an electric field higher than 3.5 V/mu m. Photoluminescence measurements showed that the NDs emit blue-green light, while blue luminescence prevails in the CNT-ND hybrid. The quenching of green luminescence was attributed to a partial removal of oxygen-containing groups from the ND surface as the result of the hybrid synthesis.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko, T. V., Shikhov, V. N., Tikhomirov, A. A.
Заглавие : Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves
Колич.характеристики :7 с
Коллективы : section VI of the Program for Basic Research of the State Academies of Sciences [56.1.4]
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 62, Is. 3. - С. 307-313. - ISSN 1021-4437, DOI 10.1134/S1021443715020144. - ISSN 1608-3407(eISSN)
Примечания : Cited References:30. - This work was supported by the State Assignment, topic no. 56.1.4, section VI of the Program for Basic Research of the State Academies of Sciences for 2013-2020.
Предметные рубрики: LEAF SENESCENCE
ONTOGENIC APPROACH
LIGHT DEPENDENCE
PARAMETERS
Ключевые слова (''Своб.индексиров.''): triticum sativus--chlorophyll fluorescence parameters--actinic light--intensity--leaf ontogeny
Аннотация: Wheat (Triticum sativus L.) seedlings of various ages (2- to 16-day-old plants) were used to study age-dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu- orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, controlled environmental conditions, and 690 A mu mol/(m(2) s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 A mu mol/(m(2) s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for measurements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the maximal photochemical quantum yield of photosystem II (F (v)/F (m)), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non-photochemical (qN and NPQ) quenching of chlorophyll fluorescence, the F (p)/F (t) ratio, and the "vitality index" (fluorescence decrease ratio, R (fd)). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 A mu mol/(m(2) s)), age-dependent changes in NPQ, F (p)/F (t), and R (fd) were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 A mu mol/(m(2) s) revealed that R (fd), NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S.
Заглавие : Mechanisms of the effect of xenobiotics on bacterial bioluminescence
Колич.характеристики :2 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P199-200. - ISSN 1522-7235, DOI 10.1002/(SICI)1522-7243(199907/08)14:4199::AID-BIO5303.0.CO;2-X
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): xenobiotics--bioluminescence quenching--energy--electron and hydrogen transfer
Аннотация: The influence of xenobiotics on the bioluminescent enzyme system is considered in terms of molecular action on the primary physicochemical processes-energy, electron and hydrogen (e(-) + H+) transduction. Dyes, non-fluorescent chemically inert organic compounds, redox-active organic compounds and metallic salts were investigated. The influence of the different xenobiotics depends in a complex way on physicochemical characteristics of the xenobiotic molecules (spectral-luminescent characteristics, electron-acceptation energy and redox potential). Copyright (C) 1999 John Wiley & Sons, Ltd.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevskii N.A., Kolmakov V.I., Popel'nitskii V.A., Gold V.M., Dubovskaya O.P.
Заглавие : Evaluation of the effect of light intensity on the measurement of the photosynthetic rate in plankton microalgae by the chlorophyll fluorescence method
Колич.характеристики :6 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2000. - Vol. 47, Is. 6. - P820-825. - ISSN 1021-4437, DOI 10.1023/A:1026671531500
Примечания : Cited References: 21
Предметные рубрики: QUANTUM YIELDS
PHYTOPLANKTON
CHLOROPLASTS
ALGAE
Ключевые слова (''Своб.индексиров.''): fluorescence--chlorophyll--microalgae--photosynthetic rate--photosynthetic activity
Аннотация: The use of relative variable fluorescence (RVF) of chlorophyll, as measured in the presence of Diuron, an inhibitor of electron transfer, for the estimation of the photosynthetic activity of plankton microalgae was analyzed under a wide range of light intensities in the PAR region. Oxygen evolution rates (estimated by the method of light and dark bottles and the amperometric method), RVF, and chlorophyll a concentration were measured in parallel in natural algal cenoses and microecosystems. When the previously used regression equation, in the form A = b(DeltaF/F-d)CchlI, where A is O-2 evolution rate (g/(m(3) h), DeltaF/F-d is RVF (relative units), C-chl is chlorophyll a concentration (mg/m(3)), and I is light intensity (W/m(2)), was verified in the PAR region, we observed a nonlinear dependence of the correction coefficient b on I, which can be described by the formula b = 6.227 x 10(3)rootI. This result agrees with the hypothesis that chlorophyll a fluorescence quenching comprises photochemical (qQ) and energy (qE) components. On the basis of the energy model, we determined the upper limit b(max) = 0.003 for light intensity range I 4.4 W/m(2) and the lower limit b(min)
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