Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=rat<.>)

Общее количество найденных документов : 22
Показаны документы с 1 по 20

1-20 21-22

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Khlusov I.A., Volova T.G.
Заглавие : A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - С. 481-498. - ISSN 09205063 (ISSN) , DOI 10.1163/156856206776986242
Ключевые слова (''Своб.индексиров.''): biocompatibility--hydroxyapatite (ha)--phb-hydroxyapatite composite--polyhydroxyalkanoate (pha)--polyhydroxybutyrate (p(3hb))--properties--biocompatibility--differential thermal analysis--electron microscopy--free energy--interfacial energy--physical properties--surface properties--x ray analysis--biomedical application--physicochemical properties--polyhydroxyalkanoate (pha)--polyhydroxybutyrate (phb)--hydroxyapatite--hydroxyapatite--poly(3 hydroxybutyric acid)--polymer--biomaterial--hydroxybutyric acid--adhesion--animal cell--animal tissue--article--biomedicine--bone marrow cell--cell differentiation--cell growth--chemical structure--composite material--controlled study--crystallization--decomposition--electron microscopy--in vitro study--in vivo study--melting point--mouse--nonhuman--ossification--osteoblast--physical chemistry--priority journal--rat--strength--structure analysis--surface property--synthesis--temperature measurement--thermal analysis--tissue engineering--wettability--animal--biomechanics--bioremediation--bone prosthesis--cattle--cell culture--chemistry--cytology--differential scanning calorimetry--drug effect--human--materials testing--prostheses and orthoses--scanning electron microscopy--standard--wistar rat--murinae--animals--biocompatible materials--biodegradation, environmental--biomechanics--bone substitutes--cattle--cells, cultured--differential thermal analysis--durapatite--humans--hydroxybutyrates--materials testing--microscopy, electron, scanning--osteoblasts--prostheses and implants--rats--rats, wistar--surface properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Goreva A., Kalacheva G., Volova T.
Заглавие : Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - С. 2185-2203. - ISSN 09205063 (ISSN) , DOI 10.1163/092050610X537138
Ключевые слова (''Своб.индексиров.''): 14c products of polymer resorption--intravenous administration--microparticles--resorbable polyhydroxybutyrate--tissues of internal organs--3-hydroxybutyric acid--sup14/supc products of polymer resorption--adverse effect--drug-delivery systems--growth and development--high molecular weight--initial molecular weight--initial values--internal organs--intravenous administration--laboratory animals--local response--matrix--mean diameter--micro-particles--polymer degradation--polymer microparticles--preparation process--radioactivity level--residual content--resorbable--sustained-release--tail veins--wistar rat--animals--biocompatibility--degradation--histology--molecular weight--morphology--radiation--radioactivity--rats--tissue--poly(3 hydroxybutyric acid)--animal experiment--animal tissue--article--biocompatibility--concentration process--controlled study--degradation--drug delivery system--female--gel permeation chromatography--heart--kidney parenchyma--liver--lung parenchyma--molecular weight--nonhuman--organ culture--priority journal--radioactivity--rat--spleen--animals--animals, laboratory--biocompatible materials--drug delivery systems--female--infusions, intravenous--materials testing--microspheres--molecular weight--particle size--polymers--rats--rats, wistar--tissue distribution--animalia--rattus--rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Biocompatibility of polyhydroxybutyrate microspheres: In vitro and in vivo evaluation
Место публикации : Journal of Materials Science: Materials in Medicine. - 2008. - Vol. 19, Is. 6. - С. 2493-2502. - ISSN 09574530 (ISSN) , DOI 10.1007/s10856-007-3345-6
Ключевые слова (''Своб.индексиров.''): biocompatibility--drug delivery--fibroblasts--ion implantation--microspheres--polymer matrix--fibroblast cells--hydroxybutyric acid--intramuscular implantation--polyhydroxybutyrate--organic polymers--microsphere--poly(3 hydroxybutyric acid)--polymer--animal cell--animal experiment--animal tissue--article--biocompatibility--cell infiltration--controlled study--giant cell--implantation--in vitro study--in vivo study--inflammation--macrophage--mouse--nonhuman--priority journal--rat--3-hydroxybutyric acid--animals--biocompatible materials--cell survival--inflammation--materials testing--mice--microspheres--necrosis--nih 3t3 cells--polymers--rats--rats, wistar--tetrazolium salts--thiazoles--time factors--rattus norvegicus
Аннотация: Microspheres have been prepared from the resorbable linear polyester of ?-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection. В© 2008 Springer Science+Business Media, LLC.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Remmel N.N., Kratasyuk V.A., Maznyak O.M., Inzhevatkin E.V., Nefedov V.P.
Заглавие : Bioluminescent analysis of intensity of pathological oxidative processes in cells of perfused rat liver after hyperthermia
Колич.характеристики :3 с
Место публикации : Bull. Exp. Biol. Med.: CONSULTANTS BUREAU, 2003. - Vol. 135, Is. 1. - P43-45. - ISSN 0007-4888, DOI 10.1023/A:1023489727703
Примечания : Cited References: 15
Ключевые слова (''Своб.индексиров.''): bioluminescent analysis--controlled perfusion of isolated liver--hyperthermia--oxidative stress
Аннотация: We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Remmel N.N., Kratasyuk V.A., Maznyak O.M., Inzhevatkin E.V., Nefedov V.P.
Заглавие : Bioluminescent analysis of intensity of pathological oxidative processes in cells of perfused rat liver after hyperthermia
Место публикации : Bulletin of Experimental Biology and Medicine. - 2003. - Vol. 135, Is. 1. - P43-45. - ISSN 00074888 (ISSN) , DOI 10.1023/A:1023489727703
Ключевые слова (''Своб.индексиров.''): bioluminescent analysis--controlled perfusion of isolated liver--hyperthermia oxidative stress--thiobarbituric acid reactive substance--animal experiment--animal model--animal tissue--article--bioluminescence--concentration (parameters)--controlled study--correlation analysis--female--hyperthermia--in vivo study--lipid peroxidation--liver metabolism--liver perfusion--male--nonhuman--oxidative stress--oxygen consumption--pathology--rat--thermal exposure--animals--female--hepatocytes--hyperthermia, induced--liver--luminescent measurements--male--oxidative stress--perfusion--rats--animalia
Аннотация: We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Shumilova A. A., Nikolaeva E. D., Kirichenko A. K., Shishatskaya E. I.
Заглавие : Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 230-240. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.068
Примечания : Cited By :1
Ключевые слова (''Своб.индексиров.''): bacterial cellulose--composites--polyhydroxyalkanoates--3 hydroxybutyric acid 4 hydroxybutyric acid copolymer--actovegin--bacterial cellulose--cellulose--collagen type 1--copolymer--cytokeratin 10--cytokeratin 14--solcoseryl--unclassified drug--angiogenesis--animal cell--animal experiment--animal model--animal tissue--biochemical analysis--biocompatibility--burn--cell growth--controlled study--cutaneous parameters--differential scanning calorimetry--drug release--elemental analysis--female--fibroblast--histopathology--in vitro study--in vivo study--inflammation--mechanics--nonhuman--rat--review--wound healing--wound planimetry--x ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019
Scopus,
Смотреть статью,
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shadrin K. V., Morgulis I. I., Pahomova V. G., Rupenko A. P., Khlebopros R. G.
Заглавие : Characteristics of oxygen transport through the surface of the isolated perfused rat liver
Место публикации : Doklad. Biochem. Biophys. - 2015. - Vol. 464, Is. 1. - С. 298-300. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672915050075
Аннотация: It is shown that the transport of oxygen through the surface of the isolated perfused rat liver is an energy-dependent process that requires the energy of ATP hydrolysis. © 2015, Pleiades Publishing, Ltd.
Scopus,
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gordeev S.A., Puzyr A.P.
Заглавие : Degradation of P(3HB) and P(3HB-co-3HV) in biological media
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - С. 643-657. - ISSN 09205063 (ISSN) , DOI 10.1163/1568562053783678
Ключевые слова (''Своб.индексиров.''): degradation rate--fiber properties--morphology--poly(hydroxybutyrate-co-hydroxyvalerate) (p(3hb-co-3hv))--polyhydroxyalkanoates(phas)--polyhydroxybutyrate (p(3hb))--copolymers--degradation--differential scanning calorimetry--enzymes--morphology--scanning electron microscopy--tensile strength--tissue--transmission electron microscopy--degradation rate--fiber properties--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (p(3hb))--biopolymers--buffer--copolymer--poly(3 hydroxybutyric acid)--polyhydroxybutyrate hydroxyvalerate copolymer--unclassified drug--animal experiment--animal model--animal tissue--article--biodegradation--controlled study--crystal structure--fiber--giant cell--in vitro study--in vivo study--macrophage--morphology--nonhuman--ph--priority journal--rat--structure analysis--tensile strength--tissue water--weight reduction--animals--biodegradation, environmental--buffers--humans--hydrogen-ion concentration--hydroxybutyrates--macrophages--microscopy, electron, scanning--microscopy, electron, transmission--muscle, skeletal--polyesters--rats--rats, wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Goreva A.V., Voinova O.N., Kalacheva G.S., Volova T.G.
Заглавие : Distribution and resorption of polymeric microparticles in visceral organs of laboratory animals after intravenous injection
Место публикации : Bulletin of Experimental Biology and Medicine. - 2009. - Vol. 148, Is. 5. - С. 789-793. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-010-0817-3
Ключевые слова (''Своб.индексиров.''): 14c--intravenous injection--microparticles--resorbable polyhydroxybutyrate--visceral tissues--carbon 14--microsphere--polyhydroxyaminobutyric acid--polymer--unclassified drug--animal experiment--animal tissue--article--biocompatibility--biodegradability--body growth--controlled drug release--controlled study--degradation kinetics--drug delivery system--drug dosage form--experimental animal--female--liver parenchyma--molecular weight--nonhuman--particle size--physical development--radioactivity--rat--spleen--tissue distribution--tissue structure--animals--animals, laboratory--carbon radioisotopes--female--injections, intravenous--particle size--polymers--rats--rats, wistar--tissue distribution--viscera--animalia
Аннотация: Microparticles obtained by using 14C-labeled resorbable hydroxyaminobutyric acid polymer were injected into the caudal vein of laboratory animals without negative aftereffects for their growth and development and without changes in the macro- and microstructure of organs and tissues. The distribution of microparticles in the viscera and the dynamics of accumulation of carbon-containing polymer degradation products in the viscera were studied. The main targets for the particles are liver tissues, as well as renal and splenic tissues. The polymeric matrix of the microparticles is most actively destroyed in the spleen and liver. The presence of high-molecular- weight polymeric matrix in organs indicates the integrity of microparticles and the possibility of long-term (up to 12 weeks) functioning of polymeric particles in vivo. В© 2009 Springer Science+Business Media, Inc.
Scopus
Найти похожие

10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vinokurov A.P., Eremeev A.V., Setkov N.A.
Заглавие : Dobutamine prevents experimental postintoxication liver cirrhosis in mice
Колич.характеристики :4 с
Место публикации : Bull. Exp. Biol. Med.: CONSULTANTS BUREAU, 2002. - Vol. 134, Is. 1. - P43-46. - ISSN 0007-4888, DOI 10.1023/A:1020604621142
Примечания : Cited References: 14
Предметные рубрики: GROWTH-FACTOR-BETA
HEPATIC-FIBROSIS
RAT HEPATOCYTES
NOREPINEPHRINE
INJURY
CELLS
Ключевые слова (''Своб.индексиров.''): hepatocytes--dobutamine--proliferation--liver cirrhosis
Аннотация: Various chronic inflammatory and necrotic processes in the liver parenchyma are accompanied by pathological morphofunctional changes, which are associated with hepatocyte death and, hyperplasia of the connective tissue. Regeneration of the liver parenchyma should include not only prevention of fibrosis, but also stimulation of hepatocyte proliferation. The adrenoceptor agonist dobutamine stimulated proliferative activity of cultured hepatocytes and prevented the development of postintoxication liver cirrhosis in mice produced by chronic poisoning with CCl4.
WOS
Найти похожие

11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gitelson I.I.
Заглавие : In vivo toxicological evaluation of polyhydroxyalkanoates.
Место публикации : Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - С. 109-111. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): alkane--animal--animal behavior--article--cytology--drug effect--erythrocyte sedimentation rate--female--heart--kidney--liver--lymphoid tissue--male--rat--spleen--wistar rat--alkanes--animals--behavior, animal--blood sedimentation--female--heart--kidney--liver--lymphoid tissue--male--rats--rats, wistar--spleen
Scopus
Найти похожие

12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Setkov N.A., Eremeev A.V.
Заглавие : Inhibitors of protein biosynthesis can stimulate proliferation of mouse hepatocytes in vitro
Колич.характеристики :8 с
Место публикации : Biol. Bull: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 30, Is. 3. - P212-219. - ISSN 1062-3590, DOI 10.1023/A:1023843409416
Примечания : Cited References: 43
Предметные рубрики: TRANSFORMING-GROWTH-FACTOR
RAT-LIVER REGENERATION
FACTOR-BETA
DNA-SYNTHESIS
PRIMARY CULTURE
PARTIAL-HEPATECTOMY
EPITHELIAL-CELLS
EXPRESSION
MECHANISMS
MODULATION
Аннотация: Hepatocyte proliferation in the liver regenerating after partial hepatectomy ceases when the organ is restored, and the mechanism of this phenomenon is still unclear. In the experiments on fusing hepatocytes from the reoenerated mouse liver (15 days after partial hepatectomy) with NIH 3T3 mouse fibroblasts, we revealed no DNA synthesis in the nuclei of stimulated fibroblasts in heterokaryons (in the presence of hepatocyte nuclei), whereas DNA synthesis in nonfused cells was undisturbed. In this work, our purpose was to find out whether the suppression of DNA synthesis in heterokaryons could be due to the appearance in hepatocytes of some endogenous factors having an inhibitory effect on proliferation. To this end, hepatocytes from the mouse liver regenerated after partial hepatectomy were treated with cycloheximide for 1-4 h and were then fused with stimulated fibroblasts. Such a short-term treatment of hepatocytes with cycloheximide proved to result in the loss of their ability to inhibit DNA synthesis in the nuclei of stimulated or quiescent fibroblasts in heterokaryons, but hepatocytes proper actively proliferated in the medium with a low serum content (0.2%). When the mice with the liver reoenerated after partial hepatectomy were treated with a single sublethal dose of cycloheximide (3 mg/kg), their hepatocytes taken two days after this treatment had no inhibitory effect. Puromycin, another inhibitor of protein synthesis, had the same effect on hepatocytes. These results may be interpreted as evidence that the final stage of liver regeneration after damage is controlled by the factors having a C C negative effect on cell proliferation.
WOS
Найти похожие

13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gitelson I.I.
Заглавие : On the involvement of macrophages and phosphomonoesterases in the tissue response to implantation of polyhydroxyalkanoates.
Место публикации : Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - С. 116-119. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): alkane--phosphatase--animal--article--comparative study--foreign body reaction--macrophage--metabolism--physiology--rat--wistar rat--alkanes--animals--foreign-body reaction--macrophages--phosphoric monoester hydrolases--rats--rats, wistar
Scopus
Найти похожие

14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Remmel N.N., Titova N.M., Kratasyuk V.A.
Заглавие : Oxidative stress monitoring in biological samples by bioluminescent method
Место публикации : Bulletin of Experimental Biology and Medicine. - 2003. - Vol. 136, Is. 2. - P209-211. - ISSN 00074888 (ISSN) , DOI 10.1023/A:1026347830283
Ключевые слова (''Своб.индексиров.''): bioluminescent analysis--lipid peroxidation--malonic dialdehyde--oxidative stress--malonaldehyde--animal tissue--article--bioluminescence--brain tissue--controlled study--fluorescence--freeze drying--kidney parenchyma--lipid peroxidation--liver--male--monitoring--nonhuman--oxidative stress--photobacterium phosphoreum--rat--serum--animals--bacteria--biological assay--lipid peroxidation--luminescent measurements--malondialdehyde--oxidative stress--photobacterium--rats--tissue extracts--animalia--negibacteria--photobacterium--photobacterium phosphoreum
Аннотация: The integral bioluminescent biotest with lyophilized fluorescent bacteria was used for monitoring of LPO processes in tissue extracts and serum of rats exposed to stress. A relationship between the content of MDA (LPO indicator) and fluorescence of bacteria was observed in all biological samples.
Scopus
Найти похожие

15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shumilova A. A., Myltygashev M. P., Kirichenko A. K., Nikolaeva E. D., Volova T. G., Shishatskaya E. I.
Заглавие : Porous 3D implants of degradable poly-3-hydroxybutyrate used to enhance regeneration of rat cranial defect
Колич.характеристики :12 с
Коллективы : Russian Academy of Sciences [01201351505]
Место публикации : J. Biomed. Mater. Res. Part A: WILEY-BLACKWELL, 2017. - Vol. 105, Is. 2. - С. 566-577. - ISSN 1549-3296, DOI 10.1002/jbm.a.35933. - ISSN 1552-4965(eISSN)
Примечания : Cited References:53. - Contract grant sponsor: The state budget allocated to the fundamental research at the Russian Academy of Sciences; contract grant number: 01201351505
Предметные рубрики: BONE TISSUE REGENERATION
CRITICAL SIZE DEFECT
IN-VITRO
CALVARIAL
Ключевые слова (''Своб.индексиров.''): 3d implants--poly-3-hydroxybutyrate--msc differentiation--grafts for--osteogenesis--regeneration of defect cranial
Аннотация: The study describes preparation and testing of porous 3D implants of natural degradable polymer of 3-hydroxybutyric acid P(3HB) for regeneration of bone tissue defects. The ability of the P(3HB) implants to favor attachment and facilitate proliferation and directed differentiation of mesenchymal stem cells (MSCs) was studied in the culture of MSCs isolated from bone marrow and adipose tissue. Tissue-engineered hybrid systems (grafts) constructed using P(3HB) and P(3HB) in combination with osteoblasts were used in experiments on laboratory animals (n=48) with bone defect model. The defect model (5 mm in diameter) was created in the rat parietal bone, and filling of the defect by the new bone tissue was monitored in the groups of animals with P(3HB) implants, with commercial material, and without implants (negative control). Computed tomography (CT) and histologic examination showed that after 120 days, in the group with the osteoblast-seeded P(3HB) implants, the defect was completely closed; in the group with the cell-free P(3HB) implants, the remaining defect was no more than 10% of the initial one (0.5 mm); in both the negative and positive controls, the size of the defect was about 1.0-1.2 mm. These results suggest that P(3HB) has good potential as osteoplastic material for reconstructive osteogenesis. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 566-577, 2017.
WOS,
Смотреть статью
Найти похожие

16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Popova T.G.
Заглавие : Study of biological properties of polyhydroxyalkanoates in a long-term experiment in vivo
Место публикации : Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - С. 218-222. - ISSN 00063398 (ISSN) , DOI 10.1023/A:1021136203338
Ключевые слова (''Своб.индексиров.''): hemoglobin--polyhydroxyalkanoic acid--animal experiment--animal tissue--article--biocompatibility--biodegradability--biosynthesis--blood cell count--blood sampling--comparative study--controlled study--dna synthesis--elasticity--enzyme activity--enzyme mechanism--erythrocyte sedimentation rate--female--in vivo study--liver function--nonhuman--organ weight--rat--suture--animalia
Scopus
Найти похожие

17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Popova T.G.
Заглавие : Study of the biological properties of polyoxyalkanoates in the in vivo chronic experiment
Место публикации : Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 29-33. - ISSN 00258075 (ISSN)
Ключевые слова (''Своб.индексиров.''): biomaterial--polymer--animal--article--bioremediation--comparative study--female--metabolism--rat--suture--wistar rat--animals--biocompatible materials--biodegradation, environmental--female--polymers--rats--rats, wistar--sutures--biocompatibility--immunology--implants (surgical)--materials testing--organic polymers--polyoxyalkanoates--rat models--suture threads--biomaterials
Аннотация: Chronic experiments on mature Wistar rats studied the biological properties of suture threads from two types of polyoxyalkanoates (POA), such as polyoxybutyrate (POB) and the copolymer of POB and polyoxyvalerate (POV) (POB/POV) as compared to the conventional surgical material (silk and catgut). The physiological and biochemical parameters that reflect the status of the animals, including their behavior, height and development, the weight of the body and viscera, the morphology of peripheral blood, the biochemical parameters of blood, the activity of some enzymes, as well as histological findings of the viscera, and lymphoid tissue responses were assessed. POA was found to produce no negative effect on the physiological, biochemical, and functional characteristics of the animals irrespective of the duration of contact with the internal medium of the body and of the chemical composition of a material.
Scopus
Найти похожие

18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogil'naya O.A., Efremov S.N., Gitelson I.I.
Заглавие : Tissue morphogenesis under the conditions of implantation of polyhydroxybutyrate, a biodegradable polymer.
Место публикации : Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - С. 123-126. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): biomaterial--hydroxybutyric acid--polymer--animal--article--bioremediation--female--morphogenesis--rat--wistar rat--animals--biocompatible materials--biodegradation, environmental--female--hydroxybutyrates--morphogenesis--polymers--rats--rats, wistar
Scopus
Найти похожие

19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Voinova O.N., Goreva A.V., Mogilnaya O.A., Volova T.G.
Заглавие : Tissue reaction to intramuscular injection of resorbable polymer microparticles
Место публикации : Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - С. 786-790. - ISSN 00074888 (ISSN) , DOI 10.1007/s10517-007-0432-0
Ключевые слова (''Своб.индексиров.''): microencapsulation--polyhydroxybutyrate--resorbable polymers--tissue reaction--poly(3 hydroxybutyric acid)--polymer--animal experiment--animal tissue--article--cell infiltration--controlled study--drug delivery system--drug formulation--female--giant cell--inflammation--macrophage--microencapsulation--nonhuman--rat--tissue reaction--absorbable implants--animals--drug compounding--female--foreign-body reaction--injections, intramuscular--microspheres--polyesters--rats--rats, wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.
Scopus
Найти похожие

20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Efremov S.N., Puzyr' A.P., Mogil'Naya O.A.
Заглавие : Tissue response to biodegradable suture threads made of polyhydroxyalkanoates
Место публикации : Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - С. 210-217. - ISSN 00063398 (ISSN) , DOI 10.1023/A:1021184119268
Ключевые слова (''Своб.индексиров.''): acid phosphatase--alkaline phosphatase--polyhydroxyalkanoic acid--animal experiment--animal tissue--article--biocompatibility--biodegradability--controlled study--elasticity--enzyme activity--enzyme mechanism--female--histochemistry--incision--nonhuman--physical chemistry--postoperative period--rat--rigidity--suture--thickness--tissue reaction--wound healing--animalia
Scopus
Найти похожие