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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Degermendzhy A.G., Zadereev E.S., Rogozin D.Y., Prokopkin I.G., Barkhatov Y.V., Tolomeev A.P., Khromechek E.B., Janse J.H., Mooij W.M., Gulati R.D.
Заглавие : Vertical stratification of physical, chemical and biological components in two saline lakes Shira and Shunet (South Siberia, Russia)
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 619-632. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9336-6
Ключевые слова (''Своб.индексиров.''): ciliates--cryptomonas--fishless lakes--gammarus--mathematical modelling--meromictic lakes--phytoplankton--stratification--sulphur bacteria--amphipod--bacterium--biomass--ciliate--ecosystem modeling--flagellate--meromictic lake--microbial community--numerical model--physicochemical property--phytoplankton--population density--saline lake--salinity--stratification--thermocline--trophic interaction--vertical profile--zooplankton--khakassia--lake shira--lake shunet--russian federation--siberia--amphipoda--bacteria (microorganisms)--ciliophora--copepoda--cryptomonas--cryptomonas sp.--gammaridae--gammarus--gammarus lacustris--phytomastigophorea--protista--rotifera
Аннотация: A feature of meromictic lakes is that several physicochemical and biological gradients affect the vertical distribution of different organisms. The vertical stratification of physical, chemical and biological components in saline, fishless meromictic lakes Shira and Shunet (Siberia, Russia) is quite different mainly because both mean depth and maximum depth of lakes differ as well as their salinity levels differ. The chemocline of the Lake Shira, as in many meromictic lakes, is inhabited by bacterial community consisting of purple sulphur and heterotrophic bacteria. As the depth of the chemocline is variable, the bacterial community does not attain high densities. The mixolimnion in Lake Shira, which is thermally stratified in summer, also creates different habitat for various species. The distribution of phytoplankton is non-uniform with its biomass peak in the metalimnion. The distribution of zooplankton is also heterogeneous with rotifers and juvenile copepods inhabiting the warmer epilimnion and older copepods found in the cold but oxic hypolimnion. The amphipod Gammarus lacustris which can be assigned to the higher trophic link in the fishless lake's ecosystem, such as Lake Shira, is also distributed non-uniformly, with its peak density generally observed in the thermocline region. The chemocline in Lake Shunet is located at the depth of 5 m, and unlike in Lake Shira, due to a sharp salinity gradient between the mixolimnion and monimolimnion, this depth is very stable. The mixolimnion in Lake Shunet is relatively shallow and the chemocline is inhabited by (1) an extremely dense bacterial community; (2) a population of Cryptomonas sp.; and (3) ciliate community comprising several species. As the mixolimnion of Lake Shunet is not thermally stratified for long period, the phytoplankton and zooplankton populations are not vertically stratified. The gammarids, however, tend to concentrate in a narrow layer located 1-2 m above the chemocline. We believe that in addition to vertical inhomogeneities of both physicochemical parameters, biological and physical factors also play a role in maintaining these inhomogeneities. We conclude that the stratified distributions of the major food web components will have several implications for ecosystem structure and dynamics. Trophic interactions as well as mass and energy flows can be significantly impacted by such heterogeneous distributions. Species spatially separated even by relatively short distances, say a few centimetres will not directly compete. Importantly, we demonstrate that not only bacteria, phytoflagellates and ciliate tend to concentrate in thin layers but also larger-sized species such Gammarus (amphipods) can also under certain environmental conditions have stratified distribution with maxima in relatively thin layer. As the vertical structure of the lake ecosystem is rather complex in such stratified lakes as ours, the strategy of research, including sampling techniques, should consider potentially variable and non-homogeneous distributions. В© 2010 The Author(s).
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vinogradov M.E., Gitelzon I.I., Sorokin Yu.I.
Заглавие : The vertical structure of a pelagic community in the tropical ocean
Место публикации : Marine Biology. - 1970. - Vol. 6, Is. 3. - С. 187-194. - ISSN 00253162 (ISSN) , DOI 10.1007/BF00347226
Аннотация: The vertical structure of the ecosystem in the euphotic zone of the western Equatorial Pacific has been studied. The studies were based on a continuous sounding of the bioluminescence field, with simultaneous, vertically aimed sampling made with the aid of a 5l water bottle and plankton nets. The bioluminescence field has a two-maxima structure with a more pronounced and permanent lower maximum found in the oligotrophic regions as deep as 60 to 100 m. In the narrow (10 m) layer of maximum bioluminescence, the concentration of zooplankton, as well as the concentration, activity and production of bacteria and phytoplankton, are several times higher than those in immediately adjacent waters at greater or lesser depth. At the same time, the concentration of nutrient salts in that layer diminishes sharply and approaches zero in the overlying water. Analysis of the data obtained shows that the production processes above the lower maximum layer are dependent not only on the turbulent influx of nutrient salts from the underlying layers, but also on the horizontal supply from the zone of upwelling. A pelagic community, in its different time aspects from the moment of water ascending until its sinking in the convergence zone, is suggested as a common system for modelling. В© 1970 Springer-Verlag.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubovskaya O. P., Glushchenko L. A.
Заглавие : THE FIRST RECORDS OF SENECELLA SIBERICA VYSHKVARTZEVA 1994 (COPEPODA, CALANOIDA) IN LARGE NORILSK LAKES, PYASINA RIVER BASIN, CENTRAL SIBERIA
Колич.характеристики :8 с
Место публикации : Zool. Zhurnal: MAIK NAUKA-INTERPERIODICA PUBL, 2018. - Vol. 97, Is. 10. - С. 1264-1271. - ISSN 0044-5134, DOI 10.1134/S0044513418100057
Примечания : Cited References:26
Предметные рубрики: LIMNOCALANUS-MACRURUS
FRESH-WATER
MICHIGAN
ESTUARY
Аннотация: The large, deep, freshwater Norilsk lakes (Lake Lama, Lake Sobachye, Lake Glubokoe and Lake Keta) are located beyond the Arctic Circle, at the northwestern edge of the Putorana Plateau. They underwent a Pleistocene boreal marine transgression. A relict of that transgression, Limnocalanus macrurus Sars 1863 (Copepoda, Calanoida), presently dominates the crustacean zooplankton of these lakes. Sympatric with the native relict species L. macrurus, Senecella siberica Vyshkvartzeva 1994, was found in our zooplankton samples taken from the deep parts of lakes Lama and Sobachye in the summer-autumn of 2014-2016. Sampling was performed using a Juday net hauled vertically from a depth of 15, 20(25/30) and/or 50 m to the surface. All Senecella specimens were older copepodites, mainly CIV stage in Lama and Sobachye lakes in August and CV in Sobachye Lake in September; their abundance was low (25-2000 ind. m(-2)). They inhabited the central deeper regions of the lakes, contributing up to 7-14% to the total wet biomass of net zooplankton in 0-20 and 0-50 m strata. Previous sporadic and rather superficial samplings in these lakes missed Senecella due to its deep locations, low abundance levels and the absence of both the oldest copepodites and adults in summer.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Muratova E., Sukovaty A., Kornilova M.
Заглавие : The effect of radionuclide and heavy metal contamination of the Yenisei River on cytogenetics of aquatic plant Elodea canadensis
Место публикации : Radioprotection. - 2009. - Vol. 44, Is. 5. - С. 83-88. - ISSN 00338451 (ISSN) , DOI 10.1051/radiopro/20095021
Аннотация: The study was done to determine concentrations of radionuclides and heavy metals and to evaluate the frequency of chromosomal aberrations in samples of Elodea canadensis, a submerged plant, collected in different parts of the Yenisei River. Samples were collected in the area subjected to radioactive impact of theMining-and-Chemical Combine (MCC) at Zheleznogorsk and in the control areas, upstream of the MCC. The investigations showed that Elodea biomass in the area affected by MCC operation contained a long inventory of artificial radionuclides typical of the MCC discharges. Upstream of the MCC, in the control sampling areas, the sediments and the Elodea biomass contained only one artificial radionuclide - 137Cs. Thus, the exposure doses to Elodea shoots and roots upstream of the MCC are small (not more than 8 ?Gy/d) and the main contribution to them is made by natural radionuclides. At the MCC discharge site (the village of Atamanovo) and downstream of it, the total dose rate increases almost an order of magnitude, reaching its maximal values - 72 ?Gy/d for Elodea shoots and 58 ? Gy/d for roots. Cytogenetic investigations of Elodea roots showed that at the MCC discharge site (the village of Atamanovo) and downstream of it the occurrence of chromosomal aberrations in ana-telophase and metaphase cells of Elodea was considerably higher than in the control area. It is highly probable that this simultaneous dramatic increase in the total exposure rate and the occurrence of chromosomal aberrations in Elodea is associated with the radiation factor. It is suggested that Elodea is affected not only by the radiation factor but also by the chemical factor - toxicity of heavy metals. В© 2009 EDP Sciences.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubovskaya O.P., Gladyshev M.I., Gubanov V.G., Makhutova O.N.
Заглавие : Study of non-consumptive mortality of Crustacean zooplankton in a Siberian reservoir using staining for live/dead sorting and sediment traps
Место публикации : Hydrobiologia. - 2003. - Vol. 504. - С. 223-227. - ISSN 00188158 (ISSN) , DOI 10.1023/B:HYDR.0000008522.88010.45
Ключевые слова (''Своб.индексиров.''): cyclops vicinus--daphnia--live/dead sorting--non-consumptive mortality--sedimentation--reservoirs (water)--sampling--sediment traps--non-consumptive mortality--population dynamics--biodiversity--mortality--population dynamics--reservoir--sediment trap--sedimentation--zooplankton--cladocera--copepoda--crustacea--cyclopia--cyclops--cyclops vicinus--daphnia
Аннотация: We studied non-consumptive (non-predatory) mortality of Daphnia and Cyclops vicinus during four sampling seasons. Mortality estimations were based on live/dead sorting using special staining and measurements of sedimentation rates for dead individuals, depended on wind speed. Original equations were used for calculations. The estimated specific non-consumptive mortality never had biologically senseless negative values, which were often obtained on the basis of the other ways of mortality estimations, and was in a good agreement with other components of population dynamics. As found, the non-consumptive mortality was the important, often the determinant component of the zooplankton population dynamics.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubovskaya O.P., Gladyshev M.I., Gubanov V.G., Makhutova O.N.
Заглавие : Study of non-consumptive mortality of Crustacean zooplankton in a Siberian reservoir using staining for live/dead sorting and sediment traps
Колич.характеристики :5 с
Место публикации : Hydrobiologia: KLUWER ACADEMIC PUBL, 2003. - Vol. 504: 4th International Conference on Reservoir Limnology and Water Quality (AUG, 2002, CESKE BUDEJOVICE, CZECH REPUBLIC), Is. 01.03.2013. - P223-227. - ISSN 0018-8158, DOI 10.1023/B:HYDR.0000008522.88010.45
Примечания : Cited References: 19
Предметные рубрики: SEASONAL DYNAMICS
MIDSUMMER DECLINE
DAPHNIA
DETRITUS
FISH
POND
Ключевые слова (''Своб.индексиров.''): cyclops vicinus--daphnia--live/dead sorting--non-consumptive mortality--sedimentation
Аннотация: We studied non-consumptive (non-predatory) mortality of Daphnia and Cyclops vicinus during four sampling seasons. Mortality estimations were based on live/dead sorting using special staining and measurements of sedimentation rates for dead individuals, depended on wind speed. Original equations were used for calculations. The estimated specific non-consumptive mortality never had biologically senseless negative values, which were often obtained on the basis of the other ways of mortality estimations, and was in a good agreement with other components of population dynamics. As found, the non-consumptive mortality was the important, often the determinant component of the zooplankton population dynamics.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Popova T.G.
Заглавие : Study of biological properties of polyhydroxyalkanoates in a long-term experiment in vivo
Место публикации : Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - С. 218-222. - ISSN 00063398 (ISSN) , DOI 10.1023/A:1021136203338
Ключевые слова (''Своб.индексиров.''): hemoglobin--polyhydroxyalkanoic acid--animal experiment--animal tissue--article--biocompatibility--biodegradability--biosynthesis--blood cell count--blood sampling--comparative study--controlled study--dna synthesis--elasticity--enzyme activity--enzyme mechanism--erythrocyte sedimentation rate--female--in vivo study--liver function--nonhuman--organ weight--rat--suture--animalia
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Kalachova G.S., Makhutova O.N.
Заглавие : Stable isotope composition of fatty acids in organisms of different trophic levels in the yenisei river
Место публикации : PLoS ONE. - 2012. - Vol. 7, Is. 3. - Ст.e34059. - ISSN 19326203 (ISSN) , DOI 10.1371/journal.pone.0034059
Ключевые слова (''Своб.индексиров.''): carbon 13--fatty acid--carbon--apatania crymophila--article--benthos--eulimnogammarus viridis--fatty acid analysis--fatty acid desaturation--fontinalis antipyretica--food chain--food web--freshwater fish--isotope analysis--lipid composition--microalga--moss--nonhuman--pelagic zone--river ecosystem--russian federation--stable isotope analysis--thymallus arcticus--trophic level--animal--chemistry--larva--metabolism--river--bryophyta--gammaridae--trichoptera--animals--carbon isotopes--fatty acids--food chain--larva--microalgae--rivers
Аннотация: We studied four-link food chain, periphytic microalgae and water moss (producers), trichopteran larvae (consumers I), gammarids (omnivorous - consumers II) and Siberian grayling (consumers III) at a littoral site of the Yenisei River on the basis of three years monthly sampling. Analysis of bulk carbon stable isotopes and compound specific isotope analysis of fatty acids (FA) were done. As found, there was a gradual depletion in 13C contents of fatty acids, including essential FA upward the food chain. In all the trophic levels a parabolic dependence of ? 13C values of fatty acids on their degree of unsaturation/chain length occurred, with 18:2n-6 and 18:3n-3 in its lowest point. The pattern in the ? 13C differences between individual fatty acids was quite similar to that reported in literature for marine pelagic food webs. Hypotheses on isotope fractionation were suggested to explain the findings. В© 2012 Gladyshev et al.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O.V., Gladyshev M.I., Rozanov A.S., Peltek S.E., Trusova M.Y.
Заглавие : Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing
Место публикации : FEMS Microbiol. Ecol. - 2014. - Vol. 89, Is. 2. - С. 442-450. - ISSN 15746941 (ISSN) , DOI 10.1111/1574-6941.12355
Ключевые слова (''Своб.индексиров.''): 16s rrna gene--bacterial community--diversity--yenisei river--actinobacteria--bacteria (microorganisms)--cyanobacteria--proteobacteria
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section. © 2014 Federation of European Microbiological Societies.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O.V., Gladyshev M.I., Rozanov A.S., Peltek S.E., Trusova M.Y.
Заглавие : Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing
Колич.характеристики :9 с
Коллективы : Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation [11.G34.31.0014]; Siberian Federal University
Место публикации : FEMS Microbiol. Ecol.: WILEY-BLACKWELL, 2014. - Vol. 89, Is. 2. - С. 442-450. - ISSN 0168-6496, DOI 10.1111/1574-6941.12355. - ISSN 1574-6941
Примечания : Cited References: 36. - This work was supported by the Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation, agreement 11.G34.31.0014, and by the project G-1 of Siberian Federal University, carried out according to Federal tasks of the Ministry of Education and Science of Russian Federation.
Предметные рубрики: DISSOLVED ORGANIC-MATTER
INLAND WATERS
CARBON
BACTERIOPLANKTON
COMMUNITY
GREENGENES
ECOSYSTEM
RESERVOIR
PATTERNS
PRIMERS
Ключевые слова (''Своб.индексиров.''): bacterial community--diversity--16s rrna gene--yenisei river
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section.
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11.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Bolsunovsky A., Dementyev D.
Заглавие : Sediments of the Yenisei River: Monitoring of radionuclide levels and estimation of sedimentation rates
Место публикации : IAHS-AISH Publication. - 2010. - Vol. 337: Symposium on Sediment Dynamics for a Changing Future (14 June 2010 through 18 June 2010, Warsaw) Conference code: 84220. - С. 143-148
Ключевые слова (''Своб.индексиров.''): artificial and natural radionuclides--dating methods--river sediments--sedimentation rates--yenisei river--dating methods--natural radionuclides--river sediments--sedimentation rates--yenisei river--anoxic sediments--cesium--europium--lead--nuclear energy--nuclear weapons--plutonium--radioisotopes--river pollution--sedimentation--sedimentology--strontium--uranium--rivers--cesium isotope--cobalt isotope--europium--fluvial deposit--plutonium isotope--radioactive pollution--radioactive waste--radionuclide--sampling--sediment core--sediment pollution--sedimentation rate--krasnoyarsk [russian federation]--russian federation--yenisei river
Аннотация: The Yenisei River, one of the largest rivers in the world, is contaminated with artificial radionuclides released by a Russian nuclear facility producing weapon-grade plutonium, which has been in operation for many years. Examination of Yenisei River sediment samples revealed the presence of artificial radionuclides typical of radioactive discharge from the Mining-and-Chemical- Combine (MCC) nuclear facility: isotopes of europium ( 152Eu, 154Eu, and 155Eu), caesium ( 137Cs and 134Cs), 60Co, 90Sr, and transuranium elements. Maximum radionuclide concentrations in sediments remained high as far as 240 km downstream of the MCC. In sediment cores collected upstream of the MCC, ?-spectrometric measurements registered only one artificial radionuclide, 137Cs, with a maximum activity of approx. 8 Bq kg -1 dry mass. Sediments of the Yenisei River also contain natural radionuclides. Sedimentation rates in several sections of the Yenisei River were determined using, different approaches: the 210Pb dating method and the ratios of artificial radionuclides - 137Cs/ 60Co and 152Eu/ 154Eu. With increasing distance downstream of the city of Krasnoyarsk, sedimentation rates increased from 0.88 cm year -1 to 1.30-1.51 cm year -1. Copyright В© 2010 IAHS Press.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Anishchenko O.V., Gladyshev M.I., Kravchuk E.S., Ivanova E.A., Gribovskaya I.V., Sushchik N.N.
Заглавие : Seasonal variations of metal concentrations in periphyton and taxonomic composition of the algal community at a Yenisei River littoral site
Место публикации : Central European Journal of Biology. - 2010. - Vol. 5, Is. 1. - С. 125-134. - ISSN 1895104X (ISSN) , DOI 10.2478/s11535-009-0060-y
Ключевые слова (''Своб.индексиров.''): heavy metals--periphyton--seasonal dynamics--algae--chlorophyta--cyanobacteria--ulothrix zonata
Аннотация: The concentrations of metals K, Na, Ca, Mg, Fe, Mn, Zn, Cu, Ni, Pb, Co and Cr, in the water and periphyton (epilithic algal communities) were studied at a site in the middle stream of the Yenisei River (Siberia, Russia) during three years using monthly sampling frequencies. Despite considerable seasonal variations in aquatic concentrations of some metals, there was no correlation between metal contents in the water and in periphyton. Seasonal concentration variations of some metals in periphyton were related to the species (taxonomic) composition of periphytic microalgae and cyanobacteria. Enhanced levels of Ni and Co in periphyton in late autumn, winter, and early spring were likely caused by the predominance of cyanobacteria in the periphytic community, and annual maximum levels of K in periphyton in late spring and early summer were attributed to the domination of Chlorophyta, primarily Ulothrix zonata. В© Versita Warsaw and Springer-Verlag Berlin Heidelberg.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kravchuk E.S., Ivanova E.A., Ageev A.V., Kalachova G.S.
Заглавие : Seasonal dynamics of long-chain polyunsaturated fatty acids in littoral benthos in the upper Yenisei river
Место публикации : Aquatic Ecology. - 2007. - Vol. 41, Is. 2. - С. 349-365. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-006-9065-z
Ключевые слова (''Своб.индексиров.''): essential compounds--periphytonic microalgae--riverine food chain--zoobenthos--amphipod--annelid--benthos--biomass--diatom--dominance--fatty acid--food chain--larva--littoral environment--seasonal variation--eurasia--krasnoyarsk [russian federation]--russian federation--yenisei river--amphipoda--annelida--arthropoda--bacillariophyta--chironomidae--gammaridae--hexapoda--invertebrata--oligochaeta (metazoa)--trichoptera
Аннотация: We studied composition and concentrations of fatty acids (FAs) in benthos from pebbly littoral region of the Yenisei River in a sampling site near Krasnoyarsk city (Siberia, Russia) for 1 year from March 2003 to February 2004. Special attention was paid to major long-chain polyunsaturated fatty acids (PUFAs) of the ?3 family: eicosapentaenoic acid (EPA, 20:5?3) and docosahexaenoic acids (DHA, 22:6?3). In phytobenthos, which was dominated by diatoms, the annual maxima of EPA and DHA pool occurred in spring and early summer. In zoobenthos, EPA and DHA pool peaked in autumn, due mainly to an increase of the biomass of dominant taxa (gammarids) and to a moderate increase of the PUFA content per body weight. Seasonal peaks of EPA in overwintering insect larvae (chironomids and caddisflies) generally coincided with those of biomass of these larvae, while there was no such trend for amphipods and oligochaetes. In spring and early summer, the main part of ?3 PUFA, 40-97% of total amount, in the littoral region was contained in biomass of producers, i.e., benthic microalgae, and in autumn it was transferred to primary consumers-benthic invertebrates, which contained ?76-93% of total ?3 PUFAs. В© 2006 Springer Science+Business Media, B.V.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lunina O.N., Bryantseva I.A., Akimov V.N., Rusanov I.I., Rogozin D.Yu., Barinova E.S., Lysenko A.M., Pimenov N.V.
Заглавие : Seasonal changes in the structure of the anoxygenic photosynthetic bacterial community in Lake Shunet, Khakassia
Место публикации : Microbiology. - 2007. - Vol. 76, Is. 3. - С. 368-379. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261707030149
Ключевые слова (''Своб.индексиров.''): anoxygenic photosynthesis--eutrophic meromictic saline lakes--green sulfur phototrophic bacteria--purple nonsulfur phototrophic bacteria--purple sulfur phototrophic bacteria--bacteria (microorganisms)--chlorobi--chromatiaceae--halochromatium--photobacteria--prosthecochloris vibrioformis--proteobacteria--rhodospirillaceae--rhodovulum--rhodovulum euryhalinum--thiocapsa
Аннотация: Seasonal studies of the anoxygenic phototrophic bacterial community of the water column of the saline eutrophic meromictic Lake Shunet (Khakassia) were performed in 2002 (June) and 2003 (February-March and August). From the redox zone down, the lake water was of dark green color. Green sulfur bacteria predominated in every season. The maximum number of green sulfur bacteria was 107 cells/ml in summer and 106 cells/ml in winter. A multi-syringe stratification sampler was applied for the study of the fine vertical distribution of phototrophs in August 2003; the sampling was performed every 5 cm. A 5-cm-thick pink-colored water layer inhabited by purple sulfur bacteria was shown to be located above the layer of green bacteria. The species composition and ratio of purple bacterial species depended on the sampling depth and on the season. In summer, the number of purple sulfur bacteria in the layer of pink water was 1.6 ? 108 cells/ml. Their number in winter was 3 ? 105 cells/ml. In the upper oxygen-containing layer of the chemocline the cells of purple nonsulfur bacteria were detected in summer. The maximum number of nonsulfur purple bacteria, 5 ? 102 cells/ml, was recorded in August 2003. According to the results of the phylogenetic analysis of pure cultures of the isolated phototrophic bacteria, which were based on 16S rDNA sequencing, green sulfur bacteria were close to Prosthecochloris vibrioformis, purple sulfur bacteria, to Thiocapsa and Halochromatium species, and purple nonsulfur bacteria, to Rhodovulum euryhalinum and Pinkicyclus mahoneyensis. В© Nauka/Interperiodica 2007.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova L. P., Stepanyuk G. A., Eremeeva E. V., Vysotski E. S.
Заглавие : Role of certain amino acid residues of the coelenterazine-binding cavity in bioluminescence of light-sensitive Ca2+-regulated photoprotein berovin
Место публикации : Photochem. Photobiol. Sci. - 2016. - Vol. 15, Is. 5. - С. 691-704. - ISSN 1474905X (ISSN) , DOI 10.1039/c6pp00050a
Аннотация: Bright bioluminescence of ctenophores is caused by Ca2+-regulated photoproteins. Although these photoproteins are functionally identical to and share many properties of cnidarian photoproteins, like aequorin and obelin, and retain the same spatial architecture, they are extremely sensitive to light, i.e. lose the ability to bioluminesce on exposure to light over the entire absorption spectrum. In addition, the degree of identity of their amino acid sequences with those of cnidarian photoproteins is only 29.4%. This suggests that the residues involved in bioluminescence of ctenophore and cnidarian photoproteins significantly differ. Here we describe the bioluminescent properties of berovin mutants with substitution of the residues located in the photoprotein internal cavity. Since the spatial structure of berovin bound with a substrate is not determined yet, to identify these residues we have modeled it with an accommodated substrate using the structures of some cnidarian Ca2+-regulated photoproteins with bound coelenterazine or coelenteramide as templates in order to obtain an adequate sampling and to take into account all possible conformers and variants for ligand-protein docking. Based on the impact of substitutions on the bioluminescent properties and model structures we speculate that within the internal cavity of ctenophore photoproteins, coelenterazine is bound as a 2-peroxy anion adduct which is stabilized owing to Coulomb interaction with a positively charged guanidinium group of Arg41 paired with Tyr204. In this case, the bioluminescence reaction is triggered by only calcium-induced conformational changes leading to the disturbance of charge-charge interaction. © 2016 The Royal Society of Chemistry and Owner Societies.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Dementyev D.
Заглавие : Radioactive contamination of pine (Pinus sylvestris) in Krasnoyarsk (Russia) following fallout from the Fukushima accident
Место публикации : J. Environ. Radioact. - 2014. - Vol. 138. - С. 87-91. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2014.08.003
Ключевые слова (''Своб.индексиров.''): fukushima accident--pine samples--radiocesium activity ratio--radionuclides--russia--activity ratios--fukushima accidents--pinus sylvestris--radioactive contamination--russia--radioisotopes--pinus sylvestris
Аннотация: Following the Fukushima accident in March 2011, samples of pine trees (Pinus sylvestris) were collected from three sites near the city of Krasnoyarsk (Siberia, Russia) during 2011-2012 and analyzed for artificial radionuclides. Concentrations of Fukushima-derived radionuclides in the samples of pine needles in April 2011 reached 5.51 ± 0.52 Bq kg-1 131I, 0.92 ± 0.04 Bq kg-1 134Cs, and 1.51 ± 0.07 Bq kg-1 137Cs. An important finding was the detection of 134Cs from the Fukushima accident not only in the pine needles and branches but also in the new shoots in 2012, which suggested a transfer of Fukushima cesium isotopes from branches to shoots. In 2011 and 2012, the 137Cs/134Cs ratio for pine needles and branches collected in sampling areas Krasnoyarsk-1 and Krasnoyarsk-2 was greater than 1 (varying within a range of 1.2-2.6), suggesting the presence of “older“, pre-Fukushima accident 137Cs. Calculations showed that for pine samples growing in areas of the Krasnoyarskii Krai unaffected by contamination from the nuclear facility, the activity of the Fukushima-derived cesium isotopes was two-three times higher than the activity of the pre-accident 137Cs.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - С. 1029-1042. - ISSN 09205063 (ISSN) , DOI 10.1163/156856203769231547
Ключевые слова (''Своб.индексиров.''): ?-hydroxy acids--endotoxins--hemocompatibility--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--bacterium lipopolysaccharide--carbon--complement--copolymer--hydroxyacid--long chain fatty acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--valeric acid derivative--adult--article--biofilm--biotechnology--blood analysis--blood clotting--blood compatibility--cell function--chemical analysis--chemical composition--complement activation--concentration (parameters)--controlled study--gas chromatography--hemostasis--human--human cell--mass spectrometry--micromorphology--nonhuman--priority journal--purification--quantitative analysis--sampling--synthesis--thrombocyte adhesion--wautersia eutropha--biocompatible materials--blood--blood coagulation tests--chromatography, gas--complement activation--cupriavidus necator--fatty acids--humans--platelet adhesiveness--polyesters--surface properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D.Y., Trusova M.Y., Khromechek E.B., Degermendzhy A.G.
Заглавие : Microbial community of the chemocline of the meromictic Lake Shunet (Khakassia, Russia) during summer stratification
Место публикации : Microbiology. - 2010. - Vol. 79, Is. 2. - С. 253-261. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261710020189
Ключевые слова (''Своб.индексиров.''): chemocline--cyanobacteria--green sulfur bacteria--meromictic lakes--microstratification--pcr-dgge--phytoflagellates--purple sulfur bacteria--bacteria (microorganisms)--chlorobi--chromatiaceae--cryptomonas--cyanobacteria--lamprocystis purpurea--phytomastigophorea--proteobacteria--synechococcus
Аннотация: The spatio-temporal organization of the bacterial community inhabiting the chemocline of the stratified meromictic Lake Shunet (Khakassia, Russia) was investigated from May to September 2005 by means of microscopy, analysis of photosynthetic pigments, and PCR-DGGE with subsequent 16S rDNA analysis. The samples were collected with a multisyringe stratification sampler, sampling being performed every 5 cm. It was demonstrated that, during the period of investigation, there were no large changes in the bacterial community of the chlemocline, at least among the detected forms. During the whole period of study, purple sulfur bacteria related to Lamprocystis purpurea (Chromatiaceae) were predominant in the chemocline. Beneath the layer of purple bacteria, green sulfur bacteria were revealed that were phylogenetically distant from strain ShNPel02, which was previously isolated from this lake. Development of phytoflagellates of the genus Cryptomonas was observed in the upper zone of the chemocline. In the chemocline of Lake Shunet, the numbers of picoplankton cyanobacteria of the genus Synechococcus increased from May to September. It was demonstrated that the application of universal bacterial primers for DGGE resulted in the same qualitative distributional pattern of predominant species as microscopic studies. В© Pleiades Publishing, Ltd., 2010.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Anishchenko O.V., Sushchnik N.N., Kalacheva G.S., Gribovskaya I.V., Ageev A.V.
Заглавие : Influence of anthropogenic pollution on content of essential polyunsaturated fatty acids in links of food chain of river ecosystem
Место публикации : Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - С. 376-385. - ISSN 19954255 (ISSN) , DOI 10.1134/S1995425512040051
Ключевые слова (''Своб.индексиров.''): gammarus--grayling--heavy metals--polyunsaturated fatty acids--amphipod--anthropogenic effect--anthropogenic source--fatty acid--food chain--heavy metal--littoral environment--oil pollution--phenol--river system--salmonid--sampling--krasnoyarsk [russian federation]--russian federation--yenisei river--gammarus
Аннотация: In the course of monthly sampling in 2008-2010, two regions of the littoral of the Yenisei river were compared. One of these regions (conventionally pure) was situated upstream of Krasnoyarsk, while the other (conventionally polluted) was downstream of Krasnoyarsk. The concentrations of heavy metals, oil products, phenols, biogenic elements and essential polyunsaturated fatty acids (PUFAs) in various components of the river ecosystem were determined. It was discovered that the anthropogenic pollution causes a decrease in the resources of essential PUFA in the biomass of the upper links of the food chain of the river ecosystem. В© 2012 Pleiades Publishing, Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalugin I. A., Darin A. V., Babich V. V., Markovich T. I., Rakshun Y. V., Darin F. A., Sorokoletov D. S., Rogozin D. Y.
Заглавие : Indicators of Oxic and Anoxic Conditions in the System of the Current Sedimentation of Saline Lake Shira (Khakassia), According to High-Resolution SR XRF Data on Bottom Sediments Frozen In Situ
Место публикации : Bull. Russ. Acad. Sci. Phys.: Pleiades Publishing, 2019. - Vol. 83, Is. 2. - С. 198-203. - ISSN 10628738 (ISSN) , DOI 10.3103/S1062873819020163
Ключевые слова (''Своб.индексиров.''): fluorescence--geochemistry--lakes--lithology--terahertz waves--x rays--annual variations--anoxic conditions--bottom sediments--geochemical indicators--hydrological regime--quantitative estimates--terahertz radiation--x ray fluorescence--oxic sediments
Аннотация: Abstract: Frozen upper layers of the bottom sediment of Lake Shira are selected using special sampling equipment. Frozen samples and solid samples prepared from the upper layers of a sediment core are examined by means of X-ray fluorescence at the Local and Scanning X-Ray Fluorescence Elemental Analysis Station of the Siberian Synchrotron and Terahertz Radiation Center’s Shared Resource Center. Analytical data on the initial frozen and processed solid samples demonstrate the good repeatability of the results. Quantitative estimates of the sedimentation regimes are obtained, and geochemical indicators of the change in redox conditions are determined from the analysis data. Lithological and geochemical records are synchronized with regional seasonal and annual variations in weather and climate, and with the hydrological regime of the lake for the last 50 years. © 2019, Allerton Press, Inc.
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