Главная
Авторизация
Фамилия
Пароль
 

Базы данных


Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Область поиска
в найденном
 Найдено в других БД:Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (20)
Формат представления найденных документов:
полныйинформационныйкраткий
Отсортировать найденные документы по:
авторузаглавиюгоду изданиятипу документа
Поисковый запрос: (<.>K=spectroscopy<.>)
Общее количество найденных документов : 51
Показаны документы с 1 по 20
 1-20    21-40   41-51 
1.


   
    Constructing sustained-release herbicide formulations based on poly-3-hydroxybutyrate and natural materials as a degradable matrix / E. G. Kiselev, A. N. Boyandin, N. O. Zhila [et al.] // Pest Manag. Sci., DOI 10.1002/ps.5702. - Cited References:83. - This study was financially supported by the project 'Agropreparations of the new generation: a strategy of construction and realization' (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, 'On measures designed to attract leading scientists to the Russian institutions of higher learning'. . - ISSN 1526-498X. - ISSN 1526-4998
РУБ Agronomy + Entomology
Рубрики:
SOIL MICROBIAL COMMUNITY
   FENOXAPROP-P-ETHYL

   SLOW-RELEASE

   METRIBUZIN

Кл.слова (ненормированные):
degradation in soil -- fenoxaprop-P-ethyl -- herbicide release -- metribuzin -- physicochemical properties -- tribenuron-methyl
Аннотация: BACKGROUND The purpose of the present study was to develop ecofriendly herbicide formulations. Its main aim was to develop and investigate slow-release formulations of herbicides (metribuzin, tribenuron-methyl, and fenoxaprop-P-ethyl) of different structure, solubility, and specificity, which were loaded into a degradable matrix of poly-3-hydroxybutyrate (P(3HB)) blended with available natural materials (peat, clay, and wood flour). RESULTS Differences in the structure and physicochemical properties of the formulations were studied depending on the type of the matrix. Herbicide release and accumulation in soil were associated with the solubility of the herbicide. Fourier-transform infrared spectroscopy showed that no chemical bonds were formed between the components in the experimental formulations. Degradation of the formulations in agro-transformed soil in laboratory conditions was chiefly influenced by the shape of the specimens (granules or pellets) while the effect of the type of filler (peat, clay, or wood flour) was insignificant. The use of granules enabled more rapid accumulation of the herbicides in soil: their peak concentrations were reached after 3 weeks of incubation while the concentrations of the herbicides released from the pellets were the highest after 5-7 weeks. Loading of the herbicides into the polymer matrix composed of the slowly degraded P(3HB) and natural materials enabled both sustained function of the formulations in soil (lasting between 1.5 and >= 3 months) and stable activity of the otherwise rapidly inactivated herbicides such as tribenuron-methyl and fenoxaprop-P-ethyl. CONCLUSION The experimental herbicide formulations enabled slow release of the active ingredients to soil. (c) 2019 Society of Chemical Industry

WOS
Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Krasnoyarsk, Russia.
Inst Biophys SB RAS, Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Krasnoyarsk, Russia.
Mahatma Gandhi Univ, Int & Interuniv Ctr Nano Sci & Nano Technol, Kottayam, Kerala, India.

Доп.точки доступа:
Kiselev, Evgeniy G.; Boyandin, Anatoly N.; Zhila, Natalia O.; Prudnikova, Svetlana, V; Shumilova, Anna A.; Baranovskiy, Sergey, V; Shishatskaya, Ekaterina, I; Thomas, Sabu; Volova, Tatiana G.; Kiselev, Evgeniy; Boyandin, Anatoly; Government of the Russian Federation [074-02-2018-328, 220]

Найти похожие
2.


   
    Toxicity and antioxidant activity of fullerenol c60,70 with low number of oxygen substituents / E. S. Kovel, A. G. Kicheeva, N. G. Vnukova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 12. - Ст. 6382, DOI 10.3390/ijms22126382 . - ISSN 1661-6596
Кл.слова (ненормированные):
Antioxidant activity -- Bioluminescent assay -- Fullerenol -- Hormesis -- Reactive oxygen species -- Toxicity
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Physics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Kovel, E. S.; Kicheeva, A. G.; Vnukova, N. G.; Churilov, G. N.; Stepin, E. A.; Kudryasheva, N. S.

Найти похожие
3.


   
    Synthesis, Mass Spectroscopy Detection, and Density Functional Theory Investigations of the Gd Endohedral Complexes of C-82 Fullerenols / A. A. Shakirova, F. N. Tomilin, V. A. Pomogaev [et al.] // Computation. - 2021. - Vol. 9, Is. 5. - Ст. 58, DOI 10.3390/computation9050058. - Cited References:41. - The experimental results were funded by RFBR project No. 18-29-19003 MK. The quantum chemical study was funded by project 0721-2020-0033 of the Russian Ministry of Science and Education. The collaboration and coordination of Russian and Korean teams was supported by Collaborative NRF-RFBR grant (Korean ID: NRF-2019K2A9A1A06100125; Russian ID: Project No. 19-53-51005 NIFa RFFI-Korea) and NRF 2021R1A2C1010455 grant. . - ISSN 2079-3197
РУБ Mathematics, Interdisciplinary Applications
Рубрики:
ZETA VALENCE QUALITY
   BIOLOGICAL-ACTIVITY

   BASIS-SETS

   TOXICITY

Кл.слова (ненормированные):
endohedral fullerenes -- density functional theory -- antioxidant activity -- reactive oxygen species -- magnetic resonance imaging
Аннотация: Gd endohedral complexes of C-82 fullerenols were synthesized and mass spectrometry analysis of their composition was carried out. It was established that the synthesis yields a series of fullerenols Gd@C82Ox(OH)(y) (x = 0, 3; y = 8, 16, 24, 36, 44). The atomic and electronic structure and properties of the synthesized fullerenols were investigated using the density functional theory calculations. It was shown that the presence of endohedral gadolinium increases the reactivity of fullerenols. It is proposed that the high-spin endohedral fullerenols are promising candidates for application in magnetic resonance imaging.

WOS
Держатели документа:
Siberian Fed Univ, Dept Biophys, Sch Engn Phys & Radio Elect, Sch Petr & Gas Engn, Pr Svobodny 79, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Siberian Branch, Kirensky Inst Phys, Krasnoyarsk Sci Ctr, Akad Gorodok 50, Krasnoyarsk 660036, Russia.
Natl Res Tomsk State Univ, Dept Phys, Lenina Ave 36, Toms 634050, Russia.
Kyungpook Natl Univ, Dept Chem, 80 Daehak Ro, Daegu 41566, South Korea.
Kyungpook Natl Univ, Green Nano Mat Res Ctr, 80 Daehak Ro, Daegu 41566, South Korea.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk Sci Ctr, Akad Gorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Shakirova, Anastasia A.; Tomilin, Felix N.; Pomogaev, Vladimir A.; Vnukova, Natalia G.; Churilov, Grigory N.; Kudryasheva, Nadezhda S.; Tchaikovskaya, Olga N.; Ovchinnikov, Sergey G.; Avramov, Pavel V.; Tomilin, Felix; RFBRRussian Foundation for Basic Research (RFBR) [18-29-19003 MK]; Russian Ministry of Science and EducationMinistry of Education and Science, Russian Federation [0721-2020-0033]; Collaborative NRF-RFBR grant (Korean) [NRF-2019K2A9A1A06100125]; Collaborative NRF-RFBR grant (Russian) [19-53-51005 NIFa RFFI-Korea]; NRF [2021R1A2C1010455]

Найти похожие
4.


   
    Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory / F. N. Tomilin, A. V. Rogova, L. P. Burakova [et al.] // Photochem. Photobiol. Sci. - 2021. - Vol. 20, Is. 4. - P559-570, DOI 10.1007/s43630-021-00039-5 . - ISSN 1474-905X
Кл.слова (ненормированные):
Absorption spectra -- Absorption spectroscopy -- Blue shift -- Dihedral angle -- Substrates -- Absorption maxima -- Covalently bound -- Electronic excitation -- Linear scaling -- Mechanical methods -- Substrate complexes -- Time dependent density functional theory -- Visible absorption spectra -- Density functional theory
Аннотация: Active hydromedusan and ctenophore Ca2+-regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound 2-hydroperoxycoelenterazine (an oxygenated intermediate of coelenterazine). Whereas the absorption maximum of hydromedusan photoproteins is at 460–470 nm, ctenophore photoproteins absorb at 437 nm. Finding out a physical reason for this blue shift is the main objective of this work, and, to achieve it, the whole structure of the protein–substrate complex was optimized using a linear scaling quantum–mechanical method. Electronic excitations pertinent to the spectra of the 2-hydroperoxy adduct of coelenterazine were simulated with time-dependent density functional theory. The dihedral angle of 60° of the 6-(p-hydroxy)-phenyl group relative to the imidazopyrazinone core of 2-hydroperoxycoelenterazine molecule was found to be the key factor determining the absorption of ctenophore photoproteins at 437 nm. The residues relevant to binding of the substrate and its adopting the particular rotation were also identified. © 2021, The Author(s), under exclusive licence to European Photochemistry Association,European Society for Photobiology.

Scopus
Держатели документа:
Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/38, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodny 79 pr., Krasnoyarsk, 660041, Russian Federation
National Research Tomsk State University, Lenin Avenue 36, Tomsk, 634050, Russian Federation
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation
Kyungpook National University, 80 Daehakro, Bukgu, Daegu, 41566, South Korea
Research Center for Computational Design of Advanced Functional Materials (CD-FMat), National Institute of Advanced Industrial Science and Technology (AIST), Central 2, Umezono 1-1-1, Tsukuba, 305-8568, Japan

Доп.точки доступа:
Tomilin, F. N.; Rogova, A. V.; Burakova, L. P.; Tchaikovskaya, O. N.; Avramov, P. V.; Fedorov, D. G.; Vysotski, E. S.

Найти похожие
5.


   
    Bacterial luciferases from vibrio harveyi and photobacterium leiognathi demonstrate different conformational stability as detected by time-resolved fluorescence spectroscopy / E. V. Nemtseva, D. V. Gulnov, M. A. Gerasimova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 19. - Ст. 10449, DOI 10.3390/ijms221910449 . - ISSN 1661-6596
Кл.слова (ненормированные):
Bacterial luciferase -- Conforma-tional stability -- FRET -- Molecular dynamics -- Time-resolved spectroscopy -- Tryptophan fluorescence -- Unfolding pathway -- Urea-induced denaturation
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of ?-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Photobiology Laboratory, Institute of Biophysics SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290, Russian Federation

Доп.точки доступа:
Nemtseva, E. V.; Gulnov, D. V.; Gerasimova, M. A.; Sukovatyi, L. A.; Burakova, L. P.; Karuzina, N. E.; Melnik, B. S.; Kratasyuk, V. A.

Найти похожие
6.


   
    Bacterial Luciferases from Vibrio harveyi and Photobacterium leiognathi Demonstrate Different Conformational Stability as Detected by Time-Resolved Fluorescence Spectroscopy / E. V. Nemtseva, D. V. Gulnov, M. A. Gerasimova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 19. - Ст. 10449, DOI 10.3390/ijms221910449. - Cited References:45. - The research was partially funded by the Ministry of Science and Higher Education of the Russian Federation (projects No. FSRZ-2020-0006); by the RFBR and Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (projects No. 20-44-243002 and 20-44-240006); and by the RFBR (project No. 20-34-90118). . - ISSN 1422-0067
РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
TRYPTOPHAN FLUORESCENCE
   CRYSTAL-STRUCTURE

   SUBUNIT

   BIOLUMINESCENCE

Кл.слова (ненормированные):
bacterial luciferase -- urea-induced denaturation -- time-resolved -- spectroscopy -- conformational stability -- FRET -- tryptophan fluorescence -- molecular dynamics -- unfolding pathway
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of alpha-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods.



WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Photobiol Lab, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Prot Res, Pushchino 142290, Russia.

Доп.точки доступа:
Nemtseva, Elena, V; Gulnov, Dmitry, V; Gerasimova, Marina A.; Sukovatyi, Lev A.; Burakova, Ludmila P.; Karuzina, Natalya E.; Melnik, Bogdan S.; Kratasyuk, Valentina A.; Burakova, Lyudmila; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; RFBRRussian Foundation for Basic Research (RFBR) [20-34-90118]; Krasnoyarsk Regional Fund of Science [20-44-243002, 20-44-240006]; RFBRRussian Foundation for Basic Research (RFBR)

Найти похожие
7.


   
    Toxicity and Antioxidant Activity of Fullerenol C-60,C-70 with Low Number of Oxygen Substituents / E. S. Kovel, A. G. Kicheeva, N. G. Vnukova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 12. - Ст. 6382, DOI 10.3390/ijms22126382. - Cited References:93. - This research was funded by RFBR, N18-29-19003; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, N20-44-243001; and partly supported by the Program of the Federal Service for Surveillance on Consumer Rights Protection and HumanWellbeing, Fundamental Study 2020-2025 (Russian Federation). . - ISSN 1422-0067
РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
HUMIC SUBSTANCES
   DETOXIFICATION PROCESSES

   BIOLOGICAL-ACTIVITY

Кл.слова (ненормированные):
fullerenol -- toxicity -- antioxidant activity -- reactive oxygen species -- bioluminescent assay -- hormesis
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene's carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.

WOS
Держатели документа:
FRC KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
FRC KSC SB RAS, Inst Phys SB RAS, Krasnoyarsk 660036, Russia.
FRC KSC SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kovel, Ekaterina S.; Kicheeva, Arina G.; Vnukova, Natalia G.; Churilov, Grigory N.; Stepin, Evsei A.; Kudryasheva, Nadezhda S.; Kovel, Ekaterina; RFBRRussian Foundation for Basic Research (RFBR) [N18-29-19003]; RFBR, Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [N20-44-243001]; Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Fundamental Study 2020-2025 (Russian Federation)

Найти похожие
8.


   
    A new composite material based on alumina nanofibers and detonation nanodiamonds: synthesis, characterization, and sensing application / N. O. Ronzhin, E. D. Posokhina, E. V. Mikhlina [et al.] // J. Nanopart. Res. - 2021. - Vol. 23, Is. 9. - Ст. 199, DOI 10.1007/s11051-021-05309-y. - Cited References:57. - This work is partially supported by the Russian Foundation for Basic Research, Project 18-29-19078 (E. V. Mikhlina, M. M. Simunin, I. Ryzhkov). . - ISSN 1388-0764. - ISSN 1572-896X
РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
ELECTROCHEMICAL ENERGY-STORAGE
   SELECTIVE DETECTION

   PHENOL DETECTION

Кл.слова (ненормированные):
Nanodiamonds -- Alumina nanofibers -- Composite -- Indicator system -- Phenol
Аннотация: The development of inexpensive, easy-to-produce, and easy-to-use analytical tools for detection of harmful and toxic substances is a relevant research problem with direct applications in environmental monitoring and protection. In this work, we propose a novel composite material based on alumina nanofibers and detonation nanodiamonds for detection of phenol in aqueous medium. The composite material was obtained by mixing an aqueous suspension of alumina nanofibers with a diameter of 10-15 nm and a length of several microns and a hydrosol of nanodiamonds with an average cluster size of 70 nm. The mechanisms underlying the interaction of these nanomaterials are clarified and the physicochemical properties of the composite are investigated. The SEM and TEM studies show that the obtained composite has a network structure, in which clusters of nanodiamonds (10-20 nm in diameter) are distributed over the surface of nanofibers. Coupling of nanomaterials occurs due to opposite signs of their zeta potentials, which results in electrostatic attraction and subsequent chemical bonding as indicated by the X-ray photoelectron spectroscopy and simultaneous thermal analysis. The bonding apparently occurs between functional groups (mainly carboxyl) on the surface of nanodiamonds and amphoteric hydroxyl groups on the surface of alumina nanofibers. The proposed composite allows an easy-to-perform colorimetric analysis for qualitative and quantitative determination of phenol in aqueous samples with linear response over a wide range of concentrations (0.5-106 mu M). Multiple tests have shown that the composite is reusable and retains its catalytic function for at least 1 year during storage at room temperature.

WOS
Держатели документа:
Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny 79, Krasnoyarsk 660041, Russia.
Inst Computat Modelling SB RAS, Akademgorodok 50-44, Krasnoyarsk 660036, Russia.
Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia.
Fed Res Ctr KSC SB RAS, Akademgorodok 50-38, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ronzhin, Nikita O.; Posokhina, Ekaterina D.; Mikhlina, Elena, V; Mikhlin, Yuri L.; Simunin, Mikhail M.; Tarasova, Lyudmila S.; Vorobyev, Sergey A.; Bondar, Vladimir S.; Ryzhkov, Ilya I.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078]

Найти похожие
9.


   
    Traces of the Tunguska Event (1908) in Sediments of Zapovednoe Lake Based on SR–XRF Data / A. V. Darin, D. Y. Rogozin, A. V. Meydus [et al.] // Dokl. Earth Sci. - 2020. - Vol. 492, Is. 2. - P442-445, DOI 10.1134/S1028334X20060045 . - ISSN 1028-334X
Кл.слова (ненормированные):
lake sediments -- microelements -- synchrotron radiation (SR) -- Tunguska event 1908 -- X-ray fluorescent analysis (XRF) -- Catchments -- Chemical elements -- Fluorescence spectroscopy -- Lakes -- Synchrotron radiation -- Bottom sediments -- Extraterrestrial origin -- Micro-particles -- Sediment core -- Synchrotron radiation X-ray fluorescence -- Tunguska -- Water catchment -- Sediments -- chemical element -- explosion -- lacustrine deposit -- radionuclide -- sediment core -- terrigenous deposit -- wildfire -- Russian Federation -- Tunguska
Аннотация: Abstract: An anomalous layer enriched with chemical elements indicating the presence of terrigenous matter was discovered in the sediment core of Zapovednoe Lake located 60 km from the epicenter of the Tunguska event (1908) using synchrotron radiation X-ray fluorescence spectroscopy (SR–XRF). Radioisotope measurements indicate that the age of the layer is consistent with the date of the catastrophe. Apparently, the anomalous layer was formed as a result of an intense terrigenous matter inflow from the water catchment area due to massive forest falls and subsequent wildfires caused by the Tunguska event. Thus, it is established that targeted searches for microparticles of extraterrestrial origin can be carried out in the discovered and dated anomalous bottom sediment layer. © 2020, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Sobolev Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Tungusskii State Nature Reserve, Krasnoyarsk, 648490, Russian Federation
Budker Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation
Kurchatov Institute National Research Center, Moscow, 123182, Russian Federation

Доп.точки доступа:
Darin, A. V.; Rogozin, D. Y.; Meydus, A. V.; Babich, V. V.; Kalugin, I. A.; Markovich, T. I.; Rakshun, Y. V.; Darin, F. A.; Sorokoletov, D. S.; Gogin, A. A.; Senin, R. A.; Degermendzhi, A. G.

Найти похожие
10.


   
    Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites / S. Thomas, A. A. Shumilova, E. G. Kiselev [et al.] // Int. J. Biol. Macromol. - 2020. - Vol. 155. - P1373-1384, DOI 10.1016/j.ijbiomac.2019.11.112. - Cited References:38. - This studywas financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".; The surface of the samples was investigated using a scanning electron microscope Hitachi TM-3000 in the Joint Instrument Use Center at the Krasnoyarsk Scientific Center of Siberian Branch of Russian Academy of Sciences. . - ISSN 0141-8130. - ISSN 1879-0003
РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
FORMULATIONS
   POLYHYDROXYALKANOATES

   POLYHYDROXYBUTYRATE

   SOIL

Кл.слова (ненормированные):
Poly-3-hydroxybutyrate -- Biocomposite -- Physical properties -- Environmental -- degradation
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour - were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P (3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P (3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. (C) 2019 Published by Elsevier B.V.

WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
Int & Interuniv Ctr Nano Sci & Nano Technol, Kottayam, Kerala, India.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Inst Phys SB RAS, Fed Res Ctr, 43-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Thomas, Sabu; Shumilova, A. A.; Kiselev, E. G.; Baranovsky, S., V; Vasiliev, A. D.; Nemtsev, I., V; Kuzmin, Andrei Petrovich; Sukovatyi, A. G.; Avinash, R. Pai; Volova, T. G.; Nemtsev, Ivan; Government of the Russian Federation [220]; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]

Найти похожие
11.


   
    alpha-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp. / M. A. Dubinnyi, I. A. Ivanov, N. S. Rodionova [et al.] // ChemistrySelect. - 2020. - Vol. 5, Is. 42. - P13155-13159, DOI 10.1002/slct.202003075. - Cited References:49. - This work was supported by the State Assignment for Basic Research of the Russian Academy of Sciences (project no. 0356-2019-0019) and the Russian Foundation for Basic Research (project no. 19-04-00348-a). . - ISSN 2365-6549
РУБ Chemistry, Multidisciplinary
Рубрики:
STRUCTURE ELUCIDATION
   MANNOSYLATION

   TRYPTOPHAN

   PROTEIN

   COMPLEMENT

Кл.слова (ненормированные):
Bioluminescence -- Earthworm -- Henlea -- Natural products -- NMR spectroscopy
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as alpha-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp.

WOS
Держатели документа:
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, GSP-7,Miklukho Maklaya Str 16-10, Moscow 117997, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Pirogov Russian Natl Res Med Univ, 1 Ostrovityanova St, Moscow 117997, Russia.

Доп.точки доступа:
Dubinnyi, Maxim A.; Ivanov, Igor A.; Rodionova, Natalia S.; Kovalchuk, Sergey I.; Kaskova, Zinaida M.; Petushkov, Valentin N.; State Assignment for Basic Research of the Russian Academy of Sciences [0356-2019-0019]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00348-a]

Найти похожие
12.


   
    α-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp. / M. A. Dubinnyi, I. A. Ivanov, N. S. Rodionova [et al.] // ChemistrySelect. - 2020. - Vol. 5, Is. 42. - P13155-13159, DOI 10.1002/slct.202003075 . - ISSN 2365-6549
Кл.слова (ненормированные):
Bioluminescence -- Earthworm -- Henlea -- Natural products -- NMR spectroscopy
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as ?-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp. © 2020 Wiley-VCH GmbH

Scopus
Держатели документа:
Shemyakin-Ovchinnikov Institute of bioorganic chemistry, Russian academy of Sciences GSP-7, Miklukho-Maklaya str., 16/10, Moscow, 117997, Russian Federation
Institute of Biophysics, Krasnoyarsk Research Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Pirogov Russian National Research Medical University, 1 Ostrovityanova st., Moscow, 117997, Russian Federation

Доп.точки доступа:
Dubinnyi, M. A.; Ivanov, I. A.; Rodionova, N. S.; Kovalchuk, S. I.; Kaskova, Z. M.; Petushkov, V. N.

Найти похожие
13.


   
    Traces of the Tunguska Event (1908) in Sediments of Zapovednoe Lake Based on SR-XRF Data / A. V. Darin, D. Y. Rogozin, A. V. Meydus [et al.] // Dokl. Earth Sci. - 2020. - Vol. 492, Is. 2. - P442-445, DOI 10.1134/S1028334X20060045. - Cited References:10. - This study was performed as a part of a State Assignment of the Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, and supported by the Russian Foundation for Basic Research, project nos. 19-04-00320 and 19-05-50046. This study was per-formed in the Shared Research Center "Siberian Synchrotron and Terahertz Radiation Center" on the basis of the VEPP-4-VEPP-2000 Electron-Positron Collider Complex of the Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences, using equipment supported by project no. RFMEFI62119X0022. . - ISSN 1028-334X. - ISSN 1531-8354
РУБ Geosciences, Multidisciplinary

Кл.слова (ненормированные):
Tunguska event 1908 -- lake sediments -- X-ray fluorescent analysis (XRF) -- synchrotron radiation (SR) -- microelements
Аннотация: An anomalous layer enriched with chemical elements indicating the presence of terrigenous matter was discovered in the sediment core of Zapovednoe Lake located 60 km from the epicenter of the Tunguska event (1908) using synchrotron radiation X-ray fluorescence spectroscopy (SR-XRF). Radioisotope measurements indicate that the age of the layer is consistent with the date of the catastrophe. Apparently, the anomalous layer was formed as a result of an intense terrigenous matter inflow from the water catchment area due to massive forest falls and subsequent wildfires caused by the Tunguska event. Thus, it is established that targeted searches for microparticles of extraterrestrial origin can be carried out in the discovered and dated anomalous bottom sediment layer.

WOS
Держатели документа:
Russian Acad Sci, Sobolev Inst Geol & Mineral, Siberian Branch, Novosibirsk 630090, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Tungusskii State Nat Reserve, Krasnoyarsk 648490, Russia.
Russian Acad Sci, Budker Inst Nucl Phys, Siberian Branch, Novosibirsk 630090, Russia.
Kurchatov Inst Natl Res Ctr, Moscow 123182, Russia.

Доп.точки доступа:
Darin, A., V; Rogozin, D. Yu; Meydus, A., V; Babich, V. V.; Kalugin, I. A.; Markovich, T., I; Rakshun, Ya, V; Darin, F. A.; Sorokoletov, D. S.; Gogin, A. A.; Senin, R. A.; Degermendzhi, A. G.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00320, 19-05-50046]; Institute of Nuclear Physics, Siberian Branch, Russian Academy of SciencesRussian Academy of Sciences [RFMEFI62119X0022]

Найти похожие
14.


   
    Constructing Slow-Release Fungicide Formulations Based on Poly(3-hydroxybutyrate) and Natural Materials as a Degradable Matrix / T. Volova [et al.] // J. Agric. Food Chem. - 2019. - Vol. 67, Is. 33. - P9220-9231, DOI 10.1021/acs.jafc.9b01634. - Cited References:52. - This study was financially supported by Project "Agro-preparations of the New Generation: A Strategy of Construction and Realization" (Agreement 074-02-2018-328) in accordance with Resolution 220 of the Government of the Russian Federation of April 9, 2010, "On Measures Designed To Attract Leading Scientists to the Russian Institutions of Higher Learning". . - ISSN 0021-8561. - ISSN 1520-5118
РУБ Agriculture, Multidisciplinary + Chemistry, Applied + Food Science &
Рубрики:
BIODEGRADABLE POLY-3-HYDROXYBUTYRATE
   CHITOSAN NANOPARTICLES

Кл.слова (ненормированные):
poly(3-hydroxybutyrate) -- fungicides -- slow-release formulations -- antifungal activity -- degradation -- fungicide release
Аннотация: Slow-release fungicide formulations (azoxystrobin, epoxiconazole, and tebuconazole) shaped as pellets and granules in a matrix of biodegradable poly(3-hydroxybutyrate) and natural fillers (clay, wood flour, and peat) were constructed. Infrared spectroscopy showed no formation of chemical bonds between components in the experimental formulations. The formulations of pesticides had antifungal activity against Fusarium verticillioides in vitro. A study of biodegradation of the experimental fungicide formulations in the soil showed that the degradation process was mainly influenced by the type of formulation without significant influence of the type of filler. More active destruction of the granules led to a more rapid accumulation of fungicides in the soil. The content of fungicides present in the soil as a result of degradation of the formulations and fungicide release was determined by their solubility. Thus, all formulations are able to function in the soil for a long time, ensuring gradual and sustained delivery of fungicides.

WOS,
Смотреть статью,
Scopus
Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Ave, Krasnoyarsk 660041, Russia.
SB RAS, Inst Biophys, Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Mahatma Gandhi Univ, Int & Inter Univ Ctr Nanosci & Nanotechnol, Priyadarshini Hills, Kottayam 686560, Kerala, India.

Доп.точки доступа:
Volova, Tatiana; Prudnikova, Svetlana; Boyandin, Anatoly; Zhila, Natalia; Kiselev, Evgeniy; Shumilova, Anna; Baranovskiy, Sergey; Demidenko, Aleksey; Shishatskaya, Ekaterina; Thomas, Sabu; Project "Agro-preparations of the New Generation: A Strategy of Construction and Realization" [074-02-2018-328]; Resolution 220 of the Government of the Russian Federation of April 9, 2010, "On Measures Designed To Attract Leading Scientists to the Russian Institutions of Higher Learning"

Найти похожие
15.


   
    Recombinant Ca2+-regulated photoproteins of ctenophores: current knowledge and application prospects / L. P. Burakova, E. S. Vysotski // Appl. Microbiol. Biotechnol. - 2019. - Vol. 103, Is. 15. - P5929-5946, DOI 10.1007/s00253-019-09939-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Intracellular calcium -- Photoinactivation -- Absorption spectroscopy -- Alkalinity -- Animals -- Binding sites -- Cloning -- Encoding (symbols) -- Phosphorescence -- Physicochemical properties -- Signal encoding -- Amino acid sequence -- Application prospect -- Biotechnology applications -- Coelenterazine -- Intracellular calcium -- Marine animals -- Photoinactivation -- Structural feature -- Bioluminescence -- Animalia -- Cnidaria -- Ctenophora (coelenterates)
Аннотация: Bright bioluminescence of ctenophores is conditioned by Ca2+-regulated photoproteins. Although they share many properties characteristic of hydromedusan Ca2+-regulated photoproteins responsible for light emission of marine animals belonging to phylum Cnidaria, a substantial distinction still exists. The ctenophore photoproteins appeared to be extremely sensitive to light—they lose the ability for bioluminescence on exposure to light over the entire absorption spectrum. Inactivation is irreversible because keeping the inactivated photoprotein in the dark does not recover its activity. The capability to emit light can be restored only by incubation of inactivated photoprotein with coelenterazine in the dark at alkaline pH in the presence of oxygen. Although these photoproteins were discovered many years ago, only the cloning of cDNAs encoding these unique bioluminescent proteins in the early 2000s has provided a new impetus for their studies. To date, cDNAs encoding Ca2+-regulated photoproteins from four different species of luminous ctenophores have been cloned. The amino acid sequences of ctenophore photoproteins turned out to completely differ from those of hydromedusan photoproteins (identity less than 29%) though also similar to them having three EF-hand Ca2+-binding sites. At the same time, these photoproteins reveal the same two-domain scaffold characteristic of hydromedusan photoproteins. This review is an attempt to systemize and critically evaluate the data scattered through various articles regarding the structural features of recombinant light-sensitive Ca2+-regulated photoproteins of ctenophores and their bioluminescent and physicochemical properties as well as to compare them with those of hydromedusan photoproteins. In addition, we also discuss the prospects of their biotechnology applications. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

Scopus,
Смотреть статью,
WOS
Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Burakova, L. P.; Vysotski, E. S.

Найти похожие
16.


   
    Experimental approach to study the effect of mutations on the protein folding pathway / E. V. Nemtseva [et al.] // PLoS One. - 2019. - Vol. 14, Is. 1. - Ст. e0210361, DOI 10.1371/journal.pone.0210361. - Cited References:38. - The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Projects 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Project 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. . - ISSN 1932-6203
РУБ Multidisciplinary Sciences
Рубрики:
FLUORESCENCE LIFETIMES ORIGIN
   TRANSITION-STATE

   EXCHANGE

   TRYPTOPHAN

Аннотация: Is it possible to compare the physicochemical properties of a wild-type protein and its mutant form under the same conditions? Provided the mutation has destabilized the protein, it may be more correct to compare the mutant protein under native conditions to the wild-type protein destabilized with a small amount of the denaturant. In general, is it appropriate to compare the properties of proteins destabilized by different treatments: mutations, pH, temperature, and denaturants like urea? These issues have compelled us to search for methods and ways of presentation of experimental results that would allow a comparison of mutant forms of proteins under different conditions and lead to conclusions on the effect of mutations on the protein folding/unfolding pathway. We have studied equilibrium unfolding of wild-type bovine carbonic anhydrase II (BCA II) and its six mutant forms using different urea concentrations. BCA II has been already studied in detail and is a good model object for validating new techniques. In this case, time-resolved fluorescence spectroscopy was chosen as the basic research method. The main features of this experimental method allowed us to compare different stages of unfolding of studied proteins and prove experimentally that a single substitution of the amino acid in three mutant forms of BCA II affected the native state of the protein but did not change its unfolding pathway. On the contrary, the inserted disulfide bridge in three other mutant forms of BCA II affected the protein unfolding pathway. An important result of this research is that we have validated the new approach allowing investigation of the effect of mutations on the folding of globular proteins, because in this way it is possible to compare proteins in the same structural states rather than under identical conditions.

WOS,
Смотреть статью,
Scopus
Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Prot Res, Pushchino, Moscow Region, Russia.

Доп.точки доступа:
Nemtseva, Elena V.; Gerasimova, Marina A.; Melnik, Tatiana N.; Melnik, Bogdan S.; Gerasimova, Marina; Nemtseva, Elena; Ministry of Science and Education of the Russian Federation [6.7734.2017]; Russian Science Foundation [N14-24-00157]

Найти похожие
17.


   
    Constructing sustained-release herbicide formulations based on poly-3-hydroxybutyrate and natural materials as a degradable matrix / E. G. Kiselev, A. N. Boyandin, N. O. Zhila [et al.] // Pest Manage. Sci. - 2019, DOI 10.1002/ps.5702 . - Article in press. - ISSN 1526-498X
Кл.слова (ненормированные):
degradation in soil -- fenoxaprop-P-ethyl -- herbicide release -- metribuzin -- physicochemical properties -- tribenuron-methyl
Аннотация: BACKGROUND: The purpose of the present study was to develop ecofriendly herbicide formulations. Its main aim was to develop and investigate slow-release formulations of herbicides (metribuzin, tribenuron-methyl, and fenoxaprop-P-ethyl) of different structure, solubility, and specificity, which were loaded into a degradable matrix of poly-3-hydroxybutyrate (P(3HB)) blended with available natural materials (peat, clay, and wood flour). RESULTS: Differences in the structure and physicochemical properties of the formulations were studied depending on the type of the matrix. Herbicide release and accumulation in soil were associated with the solubility of the herbicide. Fourier-transform infrared spectroscopy showed that no chemical bonds were formed between the components in the experimental formulations. Degradation of the formulations in agro-transformed soil in laboratory conditions was chiefly influenced by the shape of the specimens (granules or pellets) while the effect of the type of filler (peat, clay, or wood flour) was insignificant. The use of granules enabled more rapid accumulation of the herbicides in soil: their peak concentrations were reached after 3 weeks of incubation while the concentrations of the herbicides released from the pellets were the highest after 5–7 weeks. Loading of the herbicides into the polymer matrix composed of the slowly degraded P(3HB) and natural materials enabled both sustained function of the formulations in soil (lasting between 1.5 and ?3 months) and stable activity of the otherwise rapidly inactivated herbicides such as tribenuron-methyl and fenoxaprop-P-ethyl. CONCLUSION: The experimental herbicide formulations enabled slow release of the active ingredients to soil. © 2019 Society of Chemical Industry. © 2019 Society of Chemical Industry

Scopus
Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
International and Interuniversity Centre for Nano Science and Nano Technology, Mahatma Gandhi University, Kottayam, Kerala, India

Доп.точки доступа:
Kiselev, E. G.; Boyandin, A. N.; Zhila, N. O.; Prudnikova, S. V.; Shumilova, A. A.; Baranovskiy, S. V.; Shishatskaya, E. I.; Thomas, S.; Volova, T. G.

Найти похожие
18.


   
    Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites / S. Thomas [et al.] // Int. J. Biol. Macromol. - 2019, DOI 10.1016/j.ijbiomac.2019.11.112 . - Article in press. - ISSN 0141-8130
Кл.слова (ненормированные):
Biocomposite -- Environmental degradation -- Physical properties -- Poly-3-hydroxybutyrate
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour – were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P(3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P(3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. © 2018

Scopus,
Смотреть статью
Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
International and Interuniversity Centre for Nano Science and Nano technology, Kottayam, Kerala, India
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 43/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of Russian, Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Thomas, S.; Shumilova, A. A.; Kiselev, E. G.; Baranovsky, S. V.; Vasiliev, A. D.; Nemtsev, I. V.; Kuzmin, A. P.; Sukovatyi, A. G.; Avinash, R. P.; Volova, T. G.

Найти похожие
19.


   
    Bioluminescence chemistry of fireworm Odontosyllis / A. A. Kotlobay [et al.] // Proc. Natl. Acad. Sci. U. S. A. - 2019. - Vol. 116, Is. 38. - P18911-18916, DOI 10.1073/pnas.1902095116. - Cited References:16. - We thank the late Dr. Shoji Inoue and Dr. Hisae Kakoi (Meijo University) for providing Odontosyllis materials, Sergey Shakhov for photography, and Drs. Mikhail Baranov and Andrey Mikhaylov for discussions. Some experiments were carried out using equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility. Some experiments were supported by Planta LLC. Structural and mechanistic studies were supported by Russian Science Foundation Grant 18-74-10102. Isolation, purification, and biochemical studies were supported by Russian Science Foundation Grant 16-14-00052p. B.R.B. acknowledges support from the Air Force Office of Scientific Research (FA9550-18-1-0017). . - ISSN 0027-8424
РУБ Multidisciplinary Sciences
Рубрики:
MECHANISM
   DECARBOXYLATION

   OXIDATION

Кл.слова (ненормированные):
bioluminescence -- Odontosyllis luciferin -- oxyluciferin -- heterocycles
Аннотация: Marine polychaetes Odontosyllis undecimdonta, commonly known as fireworms, emit bright blue-green bioluminescence. Until the recent identification of the Odontosyllis luciferase enzyme, little progress had been made toward characterizing the key components of this bioluminescence system. Here we present the biomolecular mechanisms of enzymatic (leading to light emission) and nonenzymatic (dark) oxidation pathways of newly described O. undecimdonta luciferin. Spectral studies, including 1D and 2D NMR spectroscopy, mass spectrometry, and X-ray diffraction, of isolated substances allowed us to characterize the luciferin as an unusual tricyclic sulfur-containing heterocycle. Odontosyllis luciferin does not share structural similarity with any other known luciferins. The structures of the Odontosyllis bioluminescent system's low molecular weight components have enabled us to propose chemical transformation pathways for the enzymatic and nonspecific oxidation of luciferin.

WOS,
Смотреть статью,
Scopus
Держатели документа:
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.
Moscow Inst Phys & Technol, Dolgoprudnyi 141701, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Pirogov Russian Natl Res Med Univ, Moscow 117997, Russia.
Russian Acad Sci, AN Nesmeyanov Inst Organoelement Cpds, Moscow 119991, Russia.
Natl Res Ctr, Kurchatov Inst, Moscow 123182, Russia.
St Petersburg Natl Res Acad Univ, Russian Acad Sci, St Petersburg 194021, Russia.
Connecticut Coll, New London, CT 06320 USA.
European Mol Biol Lab Hamburg, D-22603 Hamburg, Germany.
Chubu Univ, Dept Environm Biol, Kasugai, Aichi 4878501, Japan.

Доп.точки доступа:
Kotlobay, Alexey A.; Dubinnyi, Maxim A.; Purtov, Konstantin V.; Guglya, Elena B.; Rodionova, Natalja S.; Petushkov, Valentin N.; Bolt, Yaroslav V.; Kublitski, Vadim S.; Kaskova, Zinaida M.; Ziganshin, Rustam H.; Nelyubina, Yulia V.; Dorovatovskii, Pavel V.; Eliseev, Igor E.; Branchini, Bruce R.; Bourenkov, Gleb; Ivanov, Igor A.; Oba, Yuichi; Yampolsky, Ilia V.; Tsarkova, Aleksandra S.; Kaskova, Zinaida; Russian Science FoundationRussian Science Foundation (RSF) [18-74-10102, 16-14-00052p]; Air Force Office of Scientific ResearchUnited States Department of DefenseAir Force Office of Scientific Research (AFOSR) [FA9550-18-1-0017]

Найти похожие
20.


   
    Fluorescence lifetime components reveal kinetic intermediate states upon equilibrium denaturation of carbonic anhydrase II / E. V. Nemtseva [et al.] // Methods Appl. Fluoresc. - 2018. - Vol. 6, Is. 1. - Ст. 015006, DOI 10.1088/2050-6120/aa994a. - Cited References:28. - The study of time-resolved protein fluorescence was supported by the Ministry for Science and Education of the Russian Federation (project 6.7734.2017/BCH). Kinetic and genetic engineering studies of carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. . - ISSN 2050-6120
РУБ Chemistry, Analytical + Chemistry, Physical
Рубрики:
PROTEIN FLUORESCENCE
   TRYPTOPHAN PROTEINS

   RESIDUES

   STABILITY

Кл.слова (ненормированные):
time-resolved fluorescence spectroscopy -- carbonic anhydrase II -- protein -- intermediate states -- comparison of kinetic and equilibrium experiments -- protein fluorescence lifetime
Аннотация: In most cases, intermediate states of multistage folding proteins are not 'visible' under equilibrium conditions but are revealed in kinetic experiments. Time-resolved fluorescence spectroscopy was used in equilibrium denaturation studies. The technique allows for detecting changes in the conformation and environment of tryptophan residues in different structural elements of carbonic anhydrase II which in its turn has made it possible to study the intermediate states of carbonic anhydrase II under equilibrium conditions. The results of equilibrium and kinetic experiments using wild-type bovine carbonic anhydrase II and its mutant form with the substitution of leucine for alanine at position 139 (L139A) were compared. The obtained lifetime components of intrinsic tryptophan fluorescence allowed for revealing that, the same as in kinetic experiments, under equilibrium conditions the unfolding of carbonic anhydrase II ensues through formation of intermediate states.

WOS,
Смотреть статью,
Scopus
Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia.

Доп.точки доступа:
Nemtseva, Elena V.; Lashchuk, Olesya O.; Gerasimova, Marina A.; Melnik, Tatiana N.; Nagibina, Galina S.; Melnik, Bogdan S.; Ministry for Science and Education of the Russian Federation [6.7734.2017/BCH]; Russian Science Foundation [N14-24-00157]

Найти похожие
 1-20    21-40   41-51 
 

Другие библиотеки

© Международная Ассоциация пользователей и разработчиков электронных библиотек и новых информационных технологий
(Ассоциация ЭБНИТ)