Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Найдено в других БД:

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (20)

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=spectroscopy<.>)

Общее количество найденных документов : 51
Показаны документы с 1 по 20

1-20 21-40 41-51

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi M. A., Ivanov I. A., Rodionova N. S., Kovalchuk S. I., Kaskova Z. M., Petushkov V. N.
Заглавие : α-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp.
Место публикации : ChemistrySelect: Wiley-Blackwell, 2020. - Vol. 5, Is. 42. - С. 13155-13159. - ISSN 23656549 (ISSN), DOI 10.1002/slct.202003075
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as ?-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp. © 2020 Wiley-VCH GmbH
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tomilin F. N., Rogova A. V., Burakova L. P., Tchaikovskaya O. N., Avramov P. V., Fedorov D. G., Vysotski E. S.
Заглавие : Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory
Место публикации : Photochem. Photobiol. Sci.: Springer Nature, 2021. - Vol. 20, Is. 4. - С. 559-570. - ISSN 1474905X (ISSN), DOI 10.1007/s43630-021-00039-5
Аннотация: Active hydromedusan and ctenophore Ca2+-regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound 2-hydroperoxycoelenterazine (an oxygenated intermediate of coelenterazine). Whereas the absorption maximum of hydromedusan photoproteins is at 460–470 nm, ctenophore photoproteins absorb at 437 nm. Finding out a physical reason for this blue shift is the main objective of this work, and, to achieve it, the whole structure of the protein–substrate complex was optimized using a linear scaling quantum–mechanical method. Electronic excitations pertinent to the spectra of the 2-hydroperoxy adduct of coelenterazine were simulated with time-dependent density functional theory. The dihedral angle of 60° of the 6-(p-hydroxy)-phenyl group relative to the imidazopyrazinone core of 2-hydroperoxycoelenterazine molecule was found to be the key factor determining the absorption of ctenophore photoproteins at 437 nm. The residues relevant to binding of the substrate and its adopting the particular rotation were also identified. © 2021, The Author(s), under exclusive licence to European Photochemistry Association,European Society for Photobiology.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Van Stokkum I.H.M., Gobets B., Van Mourik F., Lee J., Van Grondelle R., Visser A.J.W.G.
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Место публикации : Journal of Physical Chemistry B. - 2003. - Vol. 107, Is. 39. - С. 10934-10939. - ISSN 15206106 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--bioluminescence--chemical relaxation--chromophores--dielectric properties--proteins--solvents--bioluminescent bacteria--dimethyl ribityl lumazine--photobacterium leiognathi--riboflavin--ultrafast fluorescence relaxation spectroscopy--fluorescence
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by ?7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., van Stokkum IHM, Gobets B..., van Mourik F..., Lee J..., van Grondelle R..., Visser AJWG
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Колич.характеристики :6 с
Место публикации : J. Phys. Chem. B: AMER CHEMICAL SOC, 2003. - Vol. 107, Is. 39. - P10934-10939. - ISSN 1520-6106, DOI 10.1021/jp034266e
Примечания : Cited References: 52
Предметные рубрики: TIME-RESOLVED FLUORESCENCE
VIBRIO-FISCHERI Y1
FEMTOSECOND SOLVATION DYNAMICS
FLAVIN ADENINE-DINUCLEOTIDE
PHOTOBACTERIUM-LEIOGNATHI
BIOLOGICAL WATER
SOLVENT DYNAMICS
DIELECTRIC-RELAXATION
MOLECULAR-DYNAMICS
TRYPTOPHAN
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by similar to7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Darin A. V., Rogozin D. Y., Meydus A. V., Babich V. V., Kalugin I. A., Markovich T. I., Rakshun Y. V., Darin F. A., Sorokoletov D. S., Gogin A. A., Senin R. A., Degermendzhi A. G.
Заглавие : Traces of the Tunguska Event (1908) in Sediments of Zapovednoe Lake Based on SR–XRF Data
Место публикации : Dokl. Earth Sci.: Pleiades Publishing, 2020. - Vol. 492, Is. 2. - С. 442-445. - ISSN 1028334X (ISSN), DOI 10.1134/S1028334X20060045
Аннотация: Abstract: An anomalous layer enriched with chemical elements indicating the presence of terrigenous matter was discovered in the sediment core of Zapovednoe Lake located 60 km from the epicenter of the Tunguska event (1908) using synchrotron radiation X-ray fluorescence spectroscopy (SR–XRF). Radioisotope measurements indicate that the age of the layer is consistent with the date of the catastrophe. Apparently, the anomalous layer was formed as a result of an intense terrigenous matter inflow from the water catchment area due to massive forest falls and subsequent wildfires caused by the Tunguska event. Thus, it is established that targeted searches for microparticles of extraterrestrial origin can be carried out in the discovered and dated anomalous bottom sediment layer. © 2020, Pleiades Publishing, Ltd.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Darin, A., V, Rogozin, D. Yu, Meydus, A., V, Babich V. V., Kalugin I. A., Markovich, T., I, Rakshun, Ya, V, Darin F. A., Sorokoletov D. S., Gogin A. A., Senin R. A., Degermendzhi A. G.
Заглавие : Traces of the Tunguska Event (1908) in Sediments of Zapovednoe Lake Based on SR-XRF Data
Колич.характеристики :4 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00320, 19-05-50046]; Institute of Nuclear Physics, Siberian Branch, Russian Academy of SciencesRussian Academy of Sciences [RFMEFI62119X0022]
Место публикации : Dokl. Earth Sci.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 492, Is. 2. - С. 442-445. - ISSN 1028-334X, DOI 10.1134/S1028334X20060045. - ISSN 1531-8354(eISSN)
Примечания : Cited References:10. - This study was performed as a part of a State Assignment of the Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, and supported by the Russian Foundation for Basic Research, project nos. 19-04-00320 and 19-05-50046. This study was per-formed in the Shared Research Center "Siberian Synchrotron and Terahertz Radiation Center" on the basis of the VEPP-4-VEPP-2000 Electron-Positron Collider Complex of the Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences, using equipment supported by project no. RFMEFI62119X0022.
Аннотация: An anomalous layer enriched with chemical elements indicating the presence of terrigenous matter was discovered in the sediment core of Zapovednoe Lake located 60 km from the epicenter of the Tunguska event (1908) using synchrotron radiation X-ray fluorescence spectroscopy (SR-XRF). Radioisotope measurements indicate that the age of the layer is consistent with the date of the catastrophe. Apparently, the anomalous layer was formed as a result of an intense terrigenous matter inflow from the water catchment area due to massive forest falls and subsequent wildfires caused by the Tunguska event. Thus, it is established that targeted searches for microparticles of extraterrestrial origin can be carried out in the discovered and dated anomalous bottom sediment layer.
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovel E. S., Kicheeva A. G., Vnukova N. G., Churilov G. N., Stepin E. A., Kudryasheva N. S.
Заглавие : Toxicity and antioxidant activity of fullerenol c60,70 with low number of oxygen substituents
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2021. - Vol. 22, Is. 12. - Ст.6382. - ISSN 16616596 (ISSN), DOI 10.3390/ijms22126382
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovel, Ekaterina S., Kicheeva, Arina G., Vnukova, Natalia G., Churilov, Grigory N., Stepin, Evsei A., Kudryasheva, Nadezhda S.
Заглавие : Toxicity and Antioxidant Activity of Fullerenol C-60,C-70 with Low Number of Oxygen Substituents
Колич.характеристики :17 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [N18-29-19003]; RFBR, Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [N20-44-243001]; Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Fundamental Study 2020-2025 (Russian Federation)
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 12. - Ст.6382. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms22126382
Примечания : Cited References:93. - This research was funded by RFBR, N18-29-19003; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, N20-44-243001; and partly supported by the Program of the Federal Service for Surveillance on Consumer Rights Protection and HumanWellbeing, Fundamental Study 2020-2025 (Russian Federation).
Предметные рубрики: HUMIC SUBSTANCES
DETOXIFICATION PROCESSES
BIOLOGICAL-ACTIVITY
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene's carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.
WOS
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid'ko A.F., Pugacheva I.Yu., Shevyrnogov A.P.
Заглавие : Time course of the spectral brightness of agricultural crops during the vegetation period in Krasnoyarsk krai
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - С. 114-117. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608020130
Ключевые слова (''Своб.индексиров.''): article--crop--histology--light--metabolism--plant--russian federation--season--spectroscopy--crops, agricultural--light--plants--russia--seasons--spectrum analysis
Scopus
Найти похожие

10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Thomas S., Shumilova A. A., Kiselev E. G., Baranovsky S. V., Vasiliev A. D., Nemtsev I. V., Kuzmin A. P., Sukovatyi A. G., Avinash R. P., Volova T. G.
Заглавие : Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Article in press. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.11.112
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour – were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P(3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P(3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. © 2018
Scopus,
Смотреть статью
Найти похожие

11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Thomas, Sabu, Shumilova A. A., Kiselev E. G., Baranovsky, S., V, Vasiliev A. D., Nemtsev, I., V, Kuzmin, Andrei Petrovich, Sukovatyi A. G., Avinash, R. Pai, Volova T. G.
Заглавие : Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites
Колич.характеристики :12 с
Коллективы : Government of the Russian Federation [220]; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER, 2020. - Vol. 155. - С. 1373-1384. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2019.11.112. - ISSN 1879-0003(eISSN)
Примечания : Cited References:38. - This studywas financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".; The surface of the samples was investigated using a scanning electron microscope Hitachi TM-3000 in the Joint Instrument Use Center at the Krasnoyarsk Scientific Center of Siberian Branch of Russian Academy of Sciences.
Предметные рубрики: FORMULATIONS
POLYHYDROXYALKANOATES
POLYHYDROXYBUTYRATE
SOIL
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour - were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P (3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P (3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. (C) 2019 Published by Elsevier B.V.
WOS
Найти похожие

12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shakirova, Anastasia A., Tomilin, Felix N., Pomogaev, Vladimir A., Vnukova, Natalia G., Churilov, Grigory N., Kudryasheva, Nadezhda S., Tchaikovskaya, Olga N., Ovchinnikov, Sergey G., Avramov, Pavel V.
Заглавие : Synthesis, Mass Spectroscopy Detection, and Density Functional Theory Investigations of the Gd Endohedral Complexes of C-82 Fullerenols
Колич.характеристики :12 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [18-29-19003 MK]; Russian Ministry of Science and EducationMinistry of Education and Science, Russian Federation [0721-2020-0033]; Collaborative NRF-RFBR grant (Korean) [NRF-2019K2A9A1A06100125]; Collaborative NRF-RFBR grant (Russian) [19-53-51005 NIFa RFFI-Korea]; NRF [2021R1A2C1010455]
Место публикации : Computation: MDPI, 2021. - Vol. 9, Is. 5. - Ст.58. - ISSN 2079-3197(eISSN), DOI 10.3390/computation9050058
Примечания : Cited References:41. - The experimental results were funded by RFBR project No. 18-29-19003 MK. The quantum chemical study was funded by project 0721-2020-0033 of the Russian Ministry of Science and Education. The collaboration and coordination of Russian and Korean teams was supported by Collaborative NRF-RFBR grant (Korean ID: NRF-2019K2A9A1A06100125; Russian ID: Project No. 19-53-51005 NIFa RFFI-Korea) and NRF 2021R1A2C1010455 grant.
Предметные рубрики: ZETA VALENCE QUALITY
BIOLOGICAL-ACTIVITY
BASIS-SETS
TOXICITY
Аннотация: Gd endohedral complexes of C-82 fullerenols were synthesized and mass spectrometry analysis of their composition was carried out. It was established that the synthesis yields a series of fullerenols Gd@C82Ox(OH)(y) (x = 0, 3; y = 8, 16, 24, 36, 44). The atomic and electronic structure and properties of the synthesized fullerenols were investigated using the density functional theory calculations. It was shown that the presence of endohedral gadolinium increases the reactivity of fullerenols. It is proposed that the high-spin endohedral fullerenols are promising candidates for application in magnetic resonance imaging.
WOS
Найти похожие

13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maillard-Schaller E., Kuettel O.M., Diederich L., Schlapbach L., Zhirnov V.V., Belobrov P.I.
Заглавие : Surface properties of nanodiamond films deposited by electrophoresis on Si(100)
Место публикации : Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - С. 805-808. - ISSN 09259635 (ISSN)
Ключевые слова (''Своб.индексиров.''): energy band diagram--nanodiamond--raman spectroscopy--surface characterization--band structure--electrodeposition--electrophoresis--hydrogen--nanostructured materials--nitrogen--oxidation--oxygen--phonons--plasma applications--silicon wafers--surface properties--dielectrophoresis--negative electron affinity (nea)--phonon confinement effect--diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.
Scopus
Найти похожие

14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Peng J.L., Bulcock S..., Belobrov P.I., Bursill L.A.
Заглавие : Surface bonding states of nano-crystalline diamond balls
Колич.характеристики :15 с
Место публикации : Int. J. Mod. Phys. B: WORLD SCIENTIFIC PUBL CO PTE LTD, 2001. - Vol. 15, Is. 31. - P4071-4085. - ISSN 0217-9792, DOI 10.1142/S0217979201007865
Примечания : Cited References: 20
Предметные рубрики: PLASMON RESPONSE
POWDER
SPECTROSCOPY
MICROSCOPY
SILICON
SI(111)
Аннотация: The rough surface of nano-crystalline diamond spheres induces surface electronic states which appear as a broadened pre-peak over approx. 15 eV at the C K-edge energy threshold for carbon in the parallel electron energy loss spectrum (PEELS). This appears to be at least partially due to 1s-pi* transitions, although typically the latter occupy a range of only 4 eV for the sp(2) edge of highly-oriented pyrollytic graphite (HOPG). No pi* electrons appear in the conduction band inside the diamond particles, where all electrons are sp(3) hybridized. PEELS data were also obtained from a chemical vapour deposited diamond film (CVDF) and gem-quality diamond for comparison with the spectra of nano-diamonds. The density of sp(2) and sp(3) states on the surface of diamond nano-crystals is calculated for simple structural models of the diamond balls, including some conjecture about surface structures. The results are used to interpret the sp(2)/sp(3) ratios measured from the PEELS spectra recorded as scans across the particles. Surface roughness at the atomic scale was also examined using high-resolution transmission electron microscopy (HRTEM) and electron nano-diffraction patterns were used to confirm the crystal structures.
WOS
Найти похожие

15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A.
Заглавие : Spectral composition of light and plant productivity
Место публикации : Advances in Space Research. - 1996. - Vol. 18, Is. 4-5. - С. 259-263. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--biology--cucumber--growth, development and aging--illumination--light--maize--photon--photosynthesis--plant--radiation exposure--spectroscopy--sunflower--tomato--wheat--cucumis sativus--helianthus--light--lighting--lycopersicon esculentum--photobiology--photons--photosynthesis--plants--spectrum analysis--triticum--zea mays
Scopus
Найти похожие

16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova L. P., Vysotski E. S.
Заглавие : Recombinant Ca2+-regulated photoproteins of ctenophores: current knowledge and application prospects
Место публикации : Appl. Microbiol. Biotechnol.: Springer Verlag, 2019. - Vol. 103, Is. 15. - С. 5929-5946. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-019-09939-0
Аннотация: Bright bioluminescence of ctenophores is conditioned by Ca2+-regulated photoproteins. Although they share many properties characteristic of hydromedusan Ca2+-regulated photoproteins responsible for light emission of marine animals belonging to phylum Cnidaria, a substantial distinction still exists. The ctenophore photoproteins appeared to be extremely sensitive to light—they lose the ability for bioluminescence on exposure to light over the entire absorption spectrum. Inactivation is irreversible because keeping the inactivated photoprotein in the dark does not recover its activity. The capability to emit light can be restored only by incubation of inactivated photoprotein with coelenterazine in the dark at alkaline pH in the presence of oxygen. Although these photoproteins were discovered many years ago, only the cloning of cDNAs encoding these unique bioluminescent proteins in the early 2000s has provided a new impetus for their studies. To date, cDNAs encoding Ca2+-regulated photoproteins from four different species of luminous ctenophores have been cloned. The amino acid sequences of ctenophore photoproteins turned out to completely differ from those of hydromedusan photoproteins (identity less than 29%) though also similar to them having three EF-hand Ca2+-binding sites. At the same time, these photoproteins reveal the same two-domain scaffold characteristic of hydromedusan photoproteins. This review is an attempt to systemize and critically evaluate the data scattered through various articles regarding the structural features of recombinant light-sensitive Ca2+-regulated photoproteins of ctenophores and their bioluminescent and physicochemical properties as well as to compare them with those of hydromedusan photoproteins. In addition, we also discuss the prospects of their biotechnology applications. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
Scopus,
Смотреть статью,
WOS
Найти похожие

17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Lee J.
Заглавие : Purification and characterization of flavoproteins and cytochromes from the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1
Место публикации : European Journal of Biochemistry. - 1997. - Vol. 245, Is. 3. - С. 790-796. - ISSN 00142956 (ISSN)
Ключевые слова (''Своб.индексиров.''): anisotropy--lumazine protein--photobacterium--thioredoxin reductase--time-resolved fluorescence--cytochrome--flavoprotein--article--bioluminescence--nonhuman--priority journal--protein analysis--protein purification--sea--vibrio--amino acid sequence--bacterial proteins--cytochromes--flavoproteins--molecular sequence data--sequence alignment--vibrio--azotobacter--bacteria (microorganisms)--escherichia coli--haemophilus--haemophilus influenza--murinae--negibacteria--photobacterium--photobacterium leiognathi--pseudomonas--uncultured marine bacterium--vibrio fischeri
Аннотация: Several flavoproteins and cytochromes that occur as major components in extracts of the yellow bioluminescence Y1 strain of the murine bacterium Vibrio fischeri have been purified and characterized with respect to their mass (SDS/PAGE) and matrix-assisted laser-desorption/ionization MS), chromatographic properties, N-terminal sequence, and spectroscopy (absorption, fluorescence emission and anisotropy decay). The investigated proteins were as follows: yellow fluorescence protein (YFP) with bound riboflavin, FMN or 6,7-dimethyl-8-ribityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflavin, or 6- methyl-7-oxo-ribityllumazine; thioredoxin reductase with FAD as ligand; and two c-type diheme cytochromes, c551 and c554. We present evidence that the riboflavin-bound YFP has an N-terminal sequence corresponding to that published for the dimeric YFP. We show that an equilibrium replacement of the riboflavin can be made with excess lumazine derivative and that lumazine- bound YFP has different bioluminescence properties to those of the lumazine protein from Photobacterium leiognathi. BFP is a different protein again, and in the bacterial lysate it occurs in multiple forms, ligated to either riboflavin, lumazine, or t he 7-oxolumazine derivative. The N-terminal sequence for BFP-shows similarities to those of the YFP proteins and to lumazine protein and riboflavin synthase from Photobacterium. BFP in any form has no bioluminescence or riboflavin-synthase activity. A 70-kDa fluorescent flavoprotein with FAD as ligand has an N-terminal sequence highly similar to those of thioredoxin reductases from Haemophilus influenza and Escherichia coli. Cytochrome contaminations in previous preparations of YFP have been removed and an identified as the two c-type cytochromes c551 and c554. Both inhibit the NADH-induced bioluminescence in the reductase/luciferase system with the luciferase from P. leiognathi and V. fischeri. The N-terminal amino acid sequence of the cytochrome (c551) corresponds to a diheme cytochrome c4. The spectral properties of c554 are similar to those of other c5 cytochromes, and both c554 and c551 have absorption spectra similar to those of the respective cytochromes from the gram-negative bacteria Pseudomonas and Azotobacter.
Scopus
Найти похожие

18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Bondar' V.S., Belobrov P.I., Bukaemskii A.A.
Заглавие : Preparation of complexes nanodiamond-protein-delta-aluminum oxide.
Место публикации : Doklady Biochemistry. - 2000. - Vol. 373, Is. 1-6. - С. 139-141. - ISSN 00124958 (ISSN)
Ключевые слова (''Своб.индексиров.''): aluminum oxide--cytochrome c--diamond--article--chemistry--electron microscopy--metabolism--spectroscopy--synthesis--aluminum oxide--cytochrome c group--diamond--microscopy, electron--spectrum analysis
Scopus
Найти похожие

19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : van Oort B..., Eremeeva E.V., Koehorst RBM, Laptenok S.P., van Amerongen H..., van Berkel WJH, Malikova N.P., Markova S.V., Vysotski E.S., Visser AJWG, Lee J...
Заглавие : Picosecond Fluorescence Relaxation Spectroscopy of the Calcium-Discharged Photoproteins Aequorin and Obelin
Колич.характеристики :6 с
Коллективы : NATO Collaborative Linkage [979229]; RFBR [09-04-12-022]; 'Stichung voor Fundamenteel Onderzock der Materic (FOM)'; NWO; Wageningen University; European Community Marie Curie Research Training Network [MRTN-CT-2005-019481]; netherlands Organization [635 000 014]
Место публикации : Biochemistry: AMER CHEMICAL SOC, 2009. - Vol. 48, Is. 44. - С. 10486-10491. - ISSN 0006-2960, DOI 10.1021/bi901436m
Примечания : Cited References: 33. - This work was supported by NATO Collaborative Linkage Grant No 979229,Grants of SB RAS and RFBR 09-04-12-022, MCB program of RAS BvO was supported by 'Stichung voor Fundamenteel Onderzock der Materic (FOM)', which is financially supported by the NWO. and by I Rubicon grant of NWO E V E was supported by Wageningen University Sandwich Ph D-Fellowship program S P L was supported by Wageningen University Sandwich Ph D.-Fellowship program, European Community Marie Curie Research Training Network MRTN-CT-2005-019481 (From FLIM to FLIN), and Computational Science Gram 635 000 014 from the netherlands Organization for Scientific Research
Предметные рубрики: CA2+-REGULATED PHOTOPROTEINS
VIOLET BIOLUMINESCENCE
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
CRYSTAL-STRUCTURE
W92F OBELIN
COELENTERAZINE
MECHANISM
EXPRESSION
PROTEINS
Аннотация: Addition of calcium tons to the Ca(2+)-regulated photoproteins, such its aequorin and obelin, produces it blue bioluminescence originating from fluorescence transition of the protein-bound product coelenteramide. The kinetics of several transient fluorescent species of the bound coelenteramide is resolved after picosecond-laser excitation and streak camera detection. The Initially formed spectral distributions at picosecond-times are broad, evidently comprised of two contributions, One at higher energy (similar to 25 000 cm(-1)) assigned as from the Ca(2+)-discharged photoprotein-bound coelenteramide in its neutral state. This component decays much more rapidly (t(1/2) similar to 2 ps) in the case of the Ca(2+)-discharged obelin than aequorin (t(1/2) similar to 30 ps). The Second component at lower energy shows several intermediates in the 150-500 ps miles. with it Final species having spectral maxima 19 400 cm(-1), bound to Ca(2+)-discharged obelin. and 2 1300 cm(-1), bound to Ca(2+)-discharged aequorin, and both have it fluorescence decay lifetime of 4 ns It is proposed that the rapid kinetics of these fluorescence transients oil the picosecond time scale, correspond to times For relaxation of the protein Structural environment of the binding cavity
Найти похожие

20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Plotnikov V.F., Shishatskaya E.I., Mironov P.V., Vasil'ev A.D.
Заглавие : Physicochemical properties of two-component polyhydroxyalkanoates, 3-hydroxybutyrate-3-hydroxyvalerate copolymers
Место публикации : Biophysics. - 2004. - Vol. 49, Is. 6. - С. 934-942. - ISSN 00063509 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydroxybutyrate-hydroxyvalerate copolymers--physicochemical properties--polyhydroxyalkanoates--structure--bacteria (microorganisms)--cupriavidus necator
Аннотация: A series of two-component polyhydroxyalkanoates composed of hydroxybutyrate-hydroxyvalerate copolymers with different monomer ratio was obtained with the use of bacteria Ralstonia eutropha B5786. The properties of the polyhydroxyalkanoates in comparison with the homopolymer of hydroxybutyric acid were studied by X-ray diffraction analysis, IR spectroscopy, differential thermal analysis, and viscometry. The ratio of crystalline to amorphous phase in the copolymers tends to unity with increasing hydroxyvalerate content. This is accompanied by a decrease in the degree of crystallinity of the copolymers from 70-80 to 45-50%, the dependence is virtually linear within the range, of hydroxyvalerate mole fraction from several to 25-30 mol%. Thermal characteristics, melting temperature (Tm) and decomposition temperature (Td), of the polyhydroxyalkanoate copolymers are lower than those for polyhydroxybutyrate, whose Tm and Td are 168-170 and 260-265В°C, respectively. Both parameters decrease to 150-160 and 200-220В°C, respectively, when the hydroxyvalerate mole fraction is raised. No distinct correlation between polymer composition and molecular weight has been revealed. Copyright В© 2004 by MAIK "Nauka/Interperiodica".
Scopus
Найти похожие