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Genetically encodable bioluminescent system from fungi / A. A. Kotlobay [et al.] // Proc. Natl. Acad. Sci. U. S. A. - 2018. - Vol. 115, Is. 50. - P12728-12732, DOI 10.1073/pnas.1803615115 . - ISSN 0027-8424
Кл.слова (ненормированные):
Bioluminescence -- Fungal luciferase -- Fungal luciferin biosynthesis
Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. © 2018 National Academy of Sciences. All Rights Reserved.

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Держатели документа:
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation
Planta LLC, Moscow, 121205, Russian Federation
Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria
Medical Research Council London Institute of Medical Sciences, Imperial College London, London, W12 0NN, United Kingdom
Centre for Genomic Regulation, Barcelona Institute for Science and Technology, Barcelona, 08003, Spain
Universitat Pompeu Fabra, Barcelona, 08003, Spain
Evrogen JSC, Moscow, 117997, Russian Federation
Institute of Biophysics, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow, 142290, Russian Federation
Pirogov Russian National Research Medical University, Moscow, 117997, Russian Federation
Biomedical Nanomaterials, National Research Technological University (MISiS), Moscow, 119049, Russian Federation
Skolkovo Institute of Science and Technology, Moscow, 121205, Russian Federation
Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil
Departamento de Oceanografia Fisica, Quimica e Geologica, Instituto Oceanografico, Universidade de Sao Paulo, Sao Paulo, 05508-120, Brazil
Department of Environmental Biology, Chubu University, Kasugai, 487-8501, Japan
Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, 08010, Spain
Departamento de Quimica Fundamental, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil

Доп.точки доступа:
Kotlobay, A. A.; Sarkisyan, K. S.; Mokrushina, Y. A.; Marcet-Houben, M.; Serebrovskaya, E. O.; Markina, N. M.; Somermeyer, L. G.; Gorokhovatsky, A. Y.; Vvedensky, A.; Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Chepurnyh, T. V.; Fakhranurova, L. I.; Guglya, E. B.; Ziganshin, R.; Tsarkova, A. S.; Kaskova, Z. M.; Shender, V.; Abakumov, M.; Abakumova, T. O.; Povolotskaya, I. S.; Eroshkin, F. M.; Zaraisky, A. G.; Mishin, A. S.; Dolgov, S. V.; Mitiouchkina, T. Y.; Kopantzev, E. P.; Waldenmaier, H. E.; Oliveira, A. G.; Oba, Y.; Barsova, E.; Bogdanova, E. A.; Gabaldon, T.; Stevani, C. V.; Lukyanov, S.; Smirnov, I. V.; Gitelson, J. I.; Kondrashov, F. A.; Yampolsky, I. V.

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Computing-feasibility study of NASA nutrition requirements as applied to a bioregenerative life support system / V. S. Kovalev, N. S. Manukovsky, A. A. Tikhomirov // Acta Astronaut. - 2019. - Vol. 159. - P371-376, DOI 10.1016/j.actaastro.2019.04.001 . - ISSN 0094-5765
Кл.слова (ненормированные):
Constraint -- Food -- Modeling -- Nutrient -- Objective function -- Amino acids -- Animals -- Food products -- Models -- NASA -- Nutrients -- Nutrition -- Proteins -- Saturated fatty acids -- Uncertainty analysis -- Vitamins -- Bioregenerative life support systems -- Constraint -- Essential amino acids -- Independent variables -- Interpretation of models -- Long duration missions -- Lower and upper bounds -- Objective functions -- Life support systems (spacecraft)
Аннотация: In view of previous studies, a list of 46 foods designated for use in bioregenerative life support system was composed. With the help of a computer program, daily sets of foods of plant and animal origin were compiled from the list of foods. The objective function of modeling was intended to minimize the discrepancy between the calculated values of nutrients in daily food sets and NASA nutrition requirements for long-duration missions. The independent variables in the model were the masses of foods restricted by the lower and upper bounds. It was established that a food set is able to comprise 10-46 foods with violation of the NASA nutrition requirements for iron, vitamin B5 and vitamin D daily intakes. Inclusion of 9 foods in a set resulted in a further violation of the NASA standards concerning saturated fat. As the number of foods in a set has increased from 10 to 22, the objective function decreased from 1.0736 to 1.0332, followed by a gradual increase to 1.1233, when the maximum number of foods was selected from the list of foods. The source of uncertainty in the interpretation of modeling results are the standard NASA intakes of magnesium, potassium, zinc, manganese, vitamin C, thiamin, riboflavin, niacin, vitamin B6, folate, vitamin B12, vitamin E, vitamin K and n-6 fatty acids, given as exact values. Varying the nutrient content of food sets did not significantly affect the value of the objective function. However, some solutions were infeasible, due to the violation of the NASA standard concerning saturated fat. Also, there were food sets in which the scores of sulfur-containing amino acids and threonine were below 100. In order to reliably maintain the scores of essential amino acids above 100 in a food set, it is necessary to maintain a mass ratio of “animal protein/total protein” equal to 2/3 in accordance with the requirement of NASA. © 2019 IAA

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Kovalev, V. S.; Manukovsky, N. S.; Tikhomirov, A. A.

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Changes in athletes’ psycho-emotional stress in the course of annual macrocycle / M. A. Gamzin [et al.] // J. Siberian Fed. Univ. Humanit. Social Sci. - 2019. - Vol. 12, Is. 6. - P988-995, DOI 10.17516/1997–1370–0436 . - ISSN 1997-1370
Кл.слова (ненормированные):
Athletes -- Electroencephalography -- Psycho-emotional stress
Аннотация: Emotions significantly influence physiological processes in the athlete’s body. The level of his/ her mental stress during training, competitions and recreation is obviously not the same. Taking into account the athletes’ well-known predisposition to alexithymia, there is a need to search for objective ways of the level of psycho-emotional stress assessment. Using the calculated Stress Index (SI), based on electroencephalogram indicators and level of situational anxiety as per the standard Spielberger-Hanin test, our work assesses and compares the differences in the athletes’ psycho-emotional state in various phases of the annual macrocycle. The study involved 155 athletes doing various sports. 96 athletes were males, their average age being 24.34 ± 3.54 years; 59 athletes were females with the average age of 23.12 ± 2.3 years. The control group consisted of 101 individuals who did not experience systematic high physical exertion, 53 of them being males with the average age of 23.17 ± 2.54, 48 — females with the average age of 22.12 ± 3.01 years. The athletes’ SI values are significantly different from those in the control group, as well as among themselves at various stages of the annual macrocycle. In the preparatory and transitional periods, the SI is significantly lower than in the control group. However, during the competitive period this index increases considerably. This is characteristic of a significant increase in the athletes’ psycho-emotional stress. There is a high positive correlation (r = 0.81) between the SI value and the number of points scored in the Spielberger-Hanin situational anxiety test. © Siberian Federal University. All rights reserved.

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Держатели документа:
Academy Fight behalf D.G. Mindiashvili, 88 Markovskogo Str., Krasnoyarsk, 660049, Russian Federation
Institute of Biophysics SB RAS, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Research Institute for Medical Problems, North, 3g Partizana Zheleznyaka Str, Krasnoyarsk, 660022, Russian Federation
Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041, Russian Federation
Reshetnev Siberian State University of Science and Technology, 31 Krasnoyarsky Rabochy, Krasnoyarsk, 660037, Russian Federation

Доп.точки доступа:
Gamzin, M. A.; Bazarin, K. P.; Savchenko, A. A.; Kakukhin, A. D.

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Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients / N. G. Menzyanova [et al.] // Toxicol. Rep. - 2019. - Vol. 6. - P74-90, DOI 10.1016/j.toxrep.2018.11.009 . - ISSN 2214-7500
Кл.слова (ненормированные):
Atherosclerosis -- Cell morphology -- Intravascular stenting -- Macrophages -- Monocytes -- Polyhydroxyalkanoates
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients. © 2018 The Authors

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Держатели документа:
Siberian Federal University, 79, Svobodny av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk, 660020, Russian Federation

Доп.точки доступа:
Menzyanova, N. G.; Pyatina, S. А.; Nikolaeva, E. D.; Shabanov, A. V.; Nemtsev, I. V.; Stolyarov, D. P.; Dryganov, D. B.; Sakhnov, E. V.; Shishatskaya, E. I.

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Development of human exometabolite deep mineralization method for closed ecosystems / A. A. Tikhomirov [et al.] // Dokl. Biochem. Biophys. - 2016. - Vol. 470, Is. 1. - P316-318, DOI 10.1134/S1607672916050021. - Cited References:9. - The study was supported by the Russian Science Foundation (project no. 14-14-00599). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics

Аннотация: Methods of physicochemical further oxidation of hardly soluble sediment obtained from "wet combustion" of human exometabolites applied to space-purpose Bio Technological Life Support Systems (BTLLS) were studied. Most hardly dissoluble sediment containing Ca, P, Mg, and other essential plant nutrition elements were shown to dissolve in H2O2 and HNO3 aqueous media activated by alternating electric current. Dissolved additional mineral elements allowed (as demonstrated for lettuce) to increase the productivity of BTLLS phototrophic unit plants more than twice, which is comparable to their productivity on standard Knop solution with balanced chemical composition. Thus, dissolved mineral elements can be involved into BTLLS turnover process and increase its closure degree.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Tikhomirov, A. A.; Trifonov, S. V.; Morozov, E. A.; Kudenko, Yu. A.; Kalacheva, G. S.; Ushakova, S. A.; Russian Science Foundation [14-14-00599]

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Meta-analysis of factors associated with omega-3 fatty acid contents of wild fish / M. I. Gladyshev [et al.] // Rev. Fish. Biol. Fish. - 2018. - Vol. 28, Is. 2. - P277-299, DOI 10.1007/s11160-017-9511-0. - Cited References:138. - The work was supported by a Russian Science Foundation Grant (No. 16-14-10001). . - ISSN 0960-3166. - ISSN 1573-5184

РУБ Fisheries + Marine & Freshwater Biology
Рубрики:
FATTY-ACID-COMPOSITION
DIETARY DOCOSAHEXAENOIC ACID
LONG-CHAIN
Кл.слова (ненормированные):
Docosahexaenoic acid -- Ecomorphological factors -- Eicosapentaenoic acid -- Nutritive value -- Phylogenetic factors
Аннотация: Fish are recognized as the main source of physiologically important omega-3 long-chain polyunsaturated fatty acids, namely, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for human nutrition. However, muscle tissue contents of these fatty acids in diverse fish species, i.e., their nutritive value for humans, varied within two orders of magnitude. We reviewed contents of EPA and DHA, measured by similar methods using an internal standard during chromatography as mg per g of wet mass in 172 fish species belonging to 16 orders, to evaluate probable variations in phylogenetic and ecological drivers. EPA + DHA content varied from 25.6 mg g(-1) of wet mass (Sardinops sagax) to 0.12 mg g(-1) (Gymnura spp.). Multidimensional redundancy analysis revealed that among phylogenetic, ecomorphological and abiotic environmental factors, the highest proportion of variation contribution belonged to the shared contribution of sets of phylogenetic and ecomorphological factors. Specifically, the highest values of EPA + DHA content were characteristic of fish belonging to the orders Clupeiformes or Salmoniformes, were pelagic fast swimmers, ate zooplankton and inhabited marine waters or migrated from fresh to marine waters (anadromous migrations). High EPA and DHA content in muscle tissues of the above species appeared to be a metabolic adaptation for fast continuous swimming. In contrast to common beliefs, our meta-analysis did not support the significant influence of higher trophic levels (piscivory) and cold environments (homeoviscous adaptation) on EPA and DHA content in fish. However, many causes of high and low levels of physiologically important fatty acids in certain fish species remained unexplained and require evaluation in future studies.

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Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Inst Biophys,Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny Av 79, Krasnoyarsk 660041, Russia.
Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Leninsky Prospect 33, Moscow 119071, Russia.
Lomonosov Moscow State Univ, Moscow 119899, Russia.

Доп.точки доступа:
Gladyshev, Michail I.; Sushchik, Nadezhda N.; Tolomeev, Alexander P.; Dgebuadze, Yury Yu; Russian Science Foundation [16-14-10001]

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Modeling production processes in forest stands: An adaptation of the Solow growth model / V. Soukhovolsky, Y. Ivanova // Forests. - 2018. - Vol. 9, Is. 7, DOI 10.3390/f9070391 . - ISSN 1999-4907
Кл.слова (ненормированные):
Components of phytomass -- Forest stands -- Modeling of growth processes -- Net primary production (NPP) -- Economics -- Needles -- Energy productions -- Forest stand -- Growth process -- Independent model -- Model of economics -- Net primary production -- Phytomass -- Production process -- Forestry -- Hexapoda
Аннотация: The model of forest stand growth proposed in this study is based on R. Solow's model of economic growth. The variables introduced into the model are the "capital" (the phytomass of the non-synthesizing tree components in the stand-the stem, roots, and branches) and the "labor" (the phytomass of the photosynthesizing tree components in the stand-leaves or needles). Root phytomass is calculated with a special independent model. The process of energy production by the trees is described with the Cobb-Douglas equation. The proposed approach is used to describe growth processes in the forest stands comprising various species in Siberia and the age dynamics of net primary production. The model can explain a number of effects (such as death of the forest stand after the needles have been consumed by defoliating insects) that cannot be explained by standard logistic models. © 2018 by the authors.

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Держатели документа:
Sukachev Institute of Forest SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Akademgorodok 50-28, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Akademgorodok 50-50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Soukhovolsky, V.; Ivanova, Y.

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Dietary buckwheat enhances sirtuin1 without calorie restriction / S. Pande, R. Ranjan, A. N. Shuvaev [et al.] // J. Cereal Sci. - 2020. - Vol. 94. - Ст. 103004, DOI 10.1016/j.jcs.2020.103004 . - ISSN 0733-5210
Кл.слова (ненормированные):
Calorie restriction -- Dietary buckwheat -- Male wistar rats -- SIRT1 expression
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg?1 total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 ± 0.05, 210 ± 7, 63.26 ± 4, and 69.89 ± 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 ± 0.03, 156 ± 23.3, 31.07 ± 2 and 47.11 ± 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 ± 3.8, ca. 10%, and 40% higher than control (56.27 ± 5.6) and CR (45.05 ± 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress. © 2020 Elsevier Ltd

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Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny Prospect 79, Krasnoyarsk, 660041, Russian Federation
Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University Named After Prof. V.F. Voino-Yasenetsky, P. Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Department of Biochemistry, Medical, Pharmaceutical & Toxicological Chemistry, Krasnoyarsk State Medical University Named After Prof. V.F. Voino-Yasenetsky, P. Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Scientific Research Institute of Medical Problems of the North, P. Zheleznyaka 3g, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Pande, S.; Ranjan, R.; Shuvaev, A. N.; Malinovskaya, N. A.; Ryazanova, M.; Salmina, A. B.; Kolenchukova, O. A.; Kratasyuk, V. A.

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Software for Matching Standard Activity Enzyme Biosensors for Soil Pollution Analysis / V. A. Kratasyuk, E. M. Kolosova, O. S. Sutormin [et al.] // Sensors. - 2021. - Vol. 21, Is. 3. - Ст. 1017, DOI 10.3390/s21031017. - Cited References:20. - This research was funded by RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, Grant number 20-44-243001 and the Ministry of Science and Higher Education of the Russian Federation, Grant number FSRZ-2020-0006. . - ISSN 1424-8220

РУБ Chemistry, Analytical + Engineering, Electrical & Electronic + Instruments

Кл.слова (ненормированные):
biosensors -- enzyme -- butyrylcholinesterase -- lactic dehydrogenase -- bacterial luciferase -- soil pollution -- software
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechol, Dept Biophys, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Photobiol Lab, Fed Res Ctr,Siberian Branch,Inst Biophys, 50-50 Akagemgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Dept High Efficiency Calculat, 26 ULK Bldg Kirensky St, Krasnoyarsk 660074, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk Res Inst Agr, Siberian Branch,Fed Res Ctr, 66 Svobodny Pr, Krasnoyarsk 660037, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechol, Dept Aquat & Terr Ecosyst, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kratasyuk, Valentina A.; Kolosova, Elizaveta M.; Sutormin, Oleg S.; Lonshakova-Mukina, Viktoriya, I; Baygin, Matvey M.; Rimatskaya, Nadezhda, V; Sukovataya, Irina E.; Shpedt, Alexander A.; RFBRRussian Foundation for Basic Research (RFBR); Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science [20-44-243001]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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10.

To the question of genetic predisposition to the development of professional sensorineural hearing loss / E. E. Bashmakova, V. V. Krasitskaya, A. D. Yushkova [и др.] // Vestn. Otorinolaringologii. - 2021. - Vol. 86, Is. 1. - С. 15-19, DOI 10.17116/otorino20218601115 . - ISSN 0042-4668
Кл.слова (ненормированные):
Bioluminescent method of SNP genotyping -- Sensorineural hearing loss -- Single-nucleotide polymorphism (SNP)
Аннотация: Objective was to study single-nucleotide polymorphisms (SNP) in CAT, NCL, HSPA1L, PCDH15, and PON2 genes and their associations with hearing impairment among the people working among noise-exposed workers of the mashine-building plant (JSC «Krasmash», Krasnoyarsk, Eastern Siberia, Russia). Materials and methods. The 443 employees of Krasmash JSC, who have been working under conditions of increased noise for at least 1 year, were surveyed and examined. A hearing study was performed by speech and tonal audiometry. Tonal audiometry was carried out in accord with according to a standard method in the frequency range 125—8000 Hz. People with chronic hearing impairment, survivors of meningitis and family history of hearing impairment were excluded from the study. The allelic composition of the studied genes was determined in the remaining group of 288 workers (study group). Polymorphisms were detected using bioluminescent method, developed by the authors earlier. The study group comprised 122 people with hearing impairment (experimental group) and 166 people without impairment (control group). Results. The genotyping results of on allelic variants rs494024 (CAT), rs7598759 (NCL), rs2227956 (HSPA1L), rs7095441 (PCDH15) and rs7785846 (PON2) showed that their frequencies in the study group did not differ and were comparable with those for the European population. No statistically significant differences were revealed in the distribution of the genotypes of the studied mutations between the experimental and control groups. Also no statistically significant associations we found between hearing impairment and availability of two or several SNPs, or these SNPs and clinical characteristics of the disease (degree of hearing impairment, tinnitus). In the group of workers with an experience of 5 to 16 years, an association was found for hearing impairment and SNP rs494024, as well as when it is combined with rs7598759. Conclusions. The associations between SNP rs7598759, rs2227956, and rs7095441 and hearing impairment were not found. In the group of workers with 5—16 year experience, this association was found for SNP rs494024, as well as when it is combined with rs7598759. Discovered associations require further study. © 2021, Media Sphera Publishing Group. All rights reserved.

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Держатели документа:
Institute of Biophysics Siberian Branch of the Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Clinical Hospital 122 named after L.G. Sokolov, FMBA of Russia, St. Petersburg, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Yushkova, A. D.; Dobrecov, K. G.; Frank, L. A.

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11.

Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats / S. Pande, R. Ranjan, M. Ryazanova [et al.] // Food Chem. - 2022. - Vol. 373. - Ст. 131507, DOI 10.1016/j.foodchem.2021.131507. - Cited References:36. - The research was supported by the Ministry of Science and Higher Education of the Russian Federation (Grant no. FSRZ-2020-0006) and Russian Ministry of Education, Post-Doctoral Program of Project "5-100" [Grant No. M 2.2.3]. Authors profusely thank R. Sabirzyanov and I.A. Denisov for the needful in the arrangement of ultrapure BPA. . - ISSN 0308-8146. - ISSN 1873-7072

РУБ Chemistry, Applied + Food Science & Technology + Nutrition & Dietetics
Рубрики:
TRANSCRIPTION
   TOXICITY
   EXTRACT
   FIBER
Кл.слова (ненормированные):
Sirtuin1 -- Buckwheat -- Bisphenol A -- Dietary intervention -- Oxidative -- stress -- Male Wistar r a t s
Аннотация: Present study investigated effect of dietary buckwheat in alleviating bisphenol A (BPA) mediated oxidative stress, concomitant sirtuin1 levels in serum, stomach, and liver of rats. Experimental group A and B ingested standard diet, C and D consumed buckwheat (30%); group A and C drank normal water, B and C had BPA contamination (10 mg L 1). Sirtuin1 mean B/A ratio nearing unity in all tissues reveals inertness of BPA towards sirtuin1. Dietary buckwheat improved sirtuin1 levels both in normal (mean C/A ratio of serum, 1.65; liver, 1.24; stomach, 1.78) and BPA fed state (mean D/B ratio of serum, 1.9; liver, 1.26; stomach, 1.75). Buckwheat augmented antioxidant status in BPA fed rats as seen in mean D/B ratio of serum (catalase, 2.4; glutathione reductase (GR), 1.33; Thiols, 1.2), liver (catalase, 2; GR, 2.5; Thiols, 1.36) and stomach (catalase, 1.31; GR, 1.5; Thiols, 1.33). Therefore, buckwheat counters BPA-led oxidative stress and modulates sirtuin1.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, Svobodny Prospect 79, Krasnoyarsk 660041, Russia.
Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, P Zheleznyaka 1, Krasnoyarsk 660022, Russia.
Res Ctr Neurol, Lab Expt Brain Cytol, Moscow, Russia.
Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk Sci Ctr SB RAS, Akad Gorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Pande, Shubhra; Ranjan, Rajeev; Ryazanova, Maria; Shuvaev, Anton N.; Salmina, Alla B.; Kratasyuk, Valentina A.; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; Russian Ministry of Education, Post-Doctoral Program of Project "5-100" [M 2.2.3]

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12.

Software for matching standard activity enzyme biosensors for soil pollution analysis / V. A. Kratasyuk, E. M. Kolosova, O. S. Sutormin [et al.] // Sensors. - 2021. - Vol. 21, Is. 3. - Ст. 1017. - P1-10, DOI 10.3390/s21031017 . - ISSN 1424-8220
Кл.слова (ненормированные):
Bacterial luciferase -- Biosensors -- Butyrylcholinesterase -- Enzyme -- Lactic dehydrogenase -- Software -- Soil pollution -- Biosensors -- Soil pollution -- Soil surveys -- Soils -- Commercial standards -- Environmental Monitoring -- Enzyme biosensors -- Enzyme systems -- Inhibitory effect -- JavaScript programming -- Soil sample -- Toxic agents -- Enzyme activity
Аннотация: This work is dedicated to developing enzyme biosensor software to solve problems regarding soil pollution analysis. An algorithm and specialised software have been developed which stores, analyses and visualises data using JavaScript programming language. The developed software is based on matching data of 51 non-commercial standard soil samples and their inhibitory effects on three enzyme systems of varying complexity. This approach is able to identify the influence of chemical properties soil samples, without toxic agents, on enzyme biosensors. Such software may find wide use in environmental monitoring. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Department of Biophysics, Institute of Fundamental Biology and Biotechology, Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russian Federation
Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 50/50 Akagemgorodok, Krasnoyarsk, 660036, Russian Federation
Department of High-Efficiency Calculations, Siberian Federal University, 26-ULK building Kirensky St, Krasnoyarsk, 660074, Russian Federation
Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk Research Institute of Agriculture, Russian Academy of Sciences, Siberian Branch, 66 Svobodny pr, Krasnoyarsk, 660037, Russian Federation
Federal Research Center ‘Krasnoyarsk Scientific Center SB RAS’, Krasnoyarsk Research Institute of Agricultural, Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Kratasyuk, V. A.; Kolosova, E. M.; Sutormin, O. S.; Lonshakova-Mukina, V. I.; Baygin, M. M.; Rimatskaya, N. V.; Sukovataya, I. E.; Shpedt, A. A.

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13.

Dietary buckwheat enhances sirtuin1 without calorie restriction / S. Pande, R. Ranjan, A. N. Shuvaev [et al.] // J. Cereal Sci. - 2020. - Vol. 94. - Ст. 103004, DOI 10.1016/j.jcs.2020.103004. - Cited References:22. - This work was supported by the Russian Foundation for Basic Research (RFBR) [Grant No. 16-06-00-439] and by the Russian Ministry of Education, Post-Doctoral Program of Project "5-100" [Grant No. M 2.2.3]. . - ISSN 0733-5210. - ISSN 1095-9963

РУБ Food Science & Technology
Рубрики:
LIFE-SPAN
   STRESS
   FLOUR
   RATS
Кл.слова (ненормированные):
Dietary buckwheat -- SIRT1 expression -- Calorie restriction -- Male wistar -- rats
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg(-1) total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 +/- 0.05, 210 +/- 7, 63.26 +/- 4, and 69.89 +/- 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 +/- 0.03, 156 +/- 23.3, 31.07 +/- 2 and 47.11 +/- 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 +/- 3.8, ca. 10%, and 40% higher than control (56.27 +/- 5.6) and CR (45.05 +/- 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, Svobodny Prospect 79, Krasnoyarsk 660041, Russia.
Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, P Zheleznyaka 1, Krasnoyarsk 660022, Russia.
Krasnoyarsk State Med Univ, Dept Biochem Med Pharmaceut & Toxicol Chem, P Zheleznyaka 1, Krasnoyarsk 660022, Russia.
Sci Res Inst Med Problems North, P Zheleznyaka 3g, Krasnoyarsk 660022, Russia.
Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Pande, Shubhra; Ranjan, Rajeev; Shuvaev, Anton N.; Malinovskaya, Natalia A.; Ryazanova, Maria; Salmina, Alla B.; Kolenchukova, Oksana A.; Kratasyuk, Valentina A.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [16-06-00-439]; Russian Ministry of EducationMinistry of Education and Science, Russian Federation [M 2.2.3]

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14.

The effect of deicing salt solutes on Moina macrocopa and Allium cepa in a toxicity test experiment / T. S. Lopatina, Y. V. Aleksandrova, O. V. Anishchenko [и др.] // Vestn. Tomsk. Gos. Univ. Biol. - 2020. - Is. 51. - С. 162-178, DOI 10.17223/19988591/51/9 . - ISSN 1998-8591
Кл.слова (ненормированные):
Aquatic ecosystems -- Cladocera, Allium-test -- Salinity -- Toxicity test
Аннотация: Chloride salts are the most commonly used deicing materials for winter maintenance of roads. Numerous studies indicate a significant increase in the salinity of aquatic ecosystems associated with the long-term use of deicing materials in countries located in cold climates. The functioning of ecosystems largely depends on salinity, since salinity is one of the key factors determining the species composition, the structure of food webs and the productivity of aquatic communities. Given the growing threat of salinization of groundwater and surface waters, it is extremely important to study the effect of deicing materials on the biota and functioning of aquatic ecosystems. The aim of this research is to determine the threshold concentrations of solutions of the deicing salt mixture “Bionord” containing sodium and calcium chlorides, at which negative effects on the development of animal and plant test objects are observed. In this study, we used the salt-containing mixture “Bionord” as a model deicer. Similarly, with the most commonly used ice melting chemicals, the «Bionord» salt mixture contains a large amount of sodium and calcium chlorides (about 85% of the total weight). To evaluate the toxicity of the deicer solutions, we used acute and chronic toxicity tests with cladoceran Moina macrocopa (Straus, 1820) (Cladocera: Moinidae) and standard onion-based test with Allium cepa L. (Liliopsida: Amaryllidaceae) (Allium-test). In acute and chronic toxicity tests with Cladocera, the females on the first day of their life (body size 0.5-0.6 mm) were placed individually in jars with aged (not less than for 72 h) tap water with a volume of 20 ml with the addition of a deicer at a certain concentration. A group of animals that was placed in the medium without the deicer was used as a control. In the acute toxicity tests, we used the following concentration of the deicer: 1.3; 2.5; 4.0, 5.0; 6.0; 8.0; 10.0 g/l. The mortality of animals was recorded 24 and 48 hours after the start of the experiment. The concentration of the deicer (LC50) at which 50% of animals was observed to die, compared to the control, was determined in the acute toxicity test. In a chronic toxicity test, animals were tested in the following range of concentrations of the deicer: 0.3; 0.6; 1.3; 2.5; 5.0; 6.0 and 8.0 g/l. The chronic toxicity test was conducted until the death of all test animals. Based on the data obtained in the chronic toxicity test, the specific growth rate of juvenile females, average fecundity, and average life span of M. macrocopa were calculated for each concentration of the deicer. Bulbs of onions of the Stuttgartenrisen variety with a diameter of 1.8 ± 0.1 cm and a weight of 2.27 ± 0.17 g were used in the onion test. Bulbs with their bottoms were placed in test tubes containing 20 ml of a solute of the deicer or tap water for 48 hours. Three bulbs were tested for each concentration and for the control. The following concentrations of the deicer were used in the onion test: 1.0; 2.5; 5.0; 7.0; 10.0; 15.0; 20.0; 50.0 g/l. The general toxic and cytotoxic effects were evaluated in the onion test. The average root length and the total root length on each bulb were used as indicators of the total toxicity of the solutions of deicer. To evaluate proliferative activity, we calculated the mitotic index as the fraction of dividing cells in the apical root meristem to the total number of cells. Based on the results of the experiments, we determined median effective mixture concentrations (EC50) at which there is a 50% decrease, compared to the control, in the values of root growth indicators: average root length, sum of root lengths on each bulb and mitotic index. Median lethal concentration (LC50) of the deicing salt determined in the 48-hour acute toxicity test with females of M. macrocopa was equal to 5.1 g/l. In the chronic test, we showed that the exposure to the solutions of the deicing salt in the range of concentrations from 0.3 to 5.0 g/l does not affect the life span, specific growth rate of juveniles and fecundity of females of M. macrocopa. The median effective concentration (EC50) of the deicing salt determined in the Allium-tests were 6.3, 5.2 and 10.4 g/l for the sum of root lengths, average root length on each bulb and proliferative activity at the tips of roots (mitotic index), respectively (See Table 1 and 2). Complete inhibition of onion root growth was observed at the concentration of the decider equal to 20 g/l, while the death of all test animals in the acute toxicity test occurred at the concentration of the deicer equal to 8,0 g/l (See Fig. 1). Thus, we demonstrated that similar concentrations of the deicer induced 50% inhibition of the growth of onion roots and 50% mortality of cladocerans. These values, in general, corresponded to a critical salinity of 5-8 % above which qualitative changes occur both in the external and internal condition of aquatic animals. The electrical conductivity of the deicer solutions, which had a negative effect on the selected test species, coincides with the previously obtained values of the electrical conductivity of sodium chloride solutions harmful to cladocerans. We can assume that the main mechanism of the effect of the deicing material that we study is associated with the biological effect of its chlorine and sodium salts. Taking this into account, the value of electrical conductivity measured for solutions of deicing salt can be used to assess its negative potential effects. We estimated that in the absence of timely cleaning, regulated by the rules for using the material, the runoff from each square meter of the treated surface can lead to the pollution of 8-13 liters of fresh water. Thus, the basic requirement for the use of deicing salts on roads is the need to comply with the cleaning regime of the treated surfaces. Otherwise, the gradual accumulation of sodium and calcium chlorides in water bodies can cause an increase in salinity which will affect the survival of freshwater aquatic organisms and lead to serious disturbances in the functioning of aquatic ecosystems. © 2020 Tomsk State University. All rights reserved.

Scopus
Держатели документа:
Laboratory of Ecosystem Biophysics, Institute of Biophysics, Federal Research Centre Krasnoyarsk Scientific Centre, Siberian Branch, Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Laboratory of Bioluminescent and Environmental Technologies, Institute of Biophysics, Federal Research Centre Krasnoyarsk Scientific Centre, Siberian Branch, Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Analytical Laboratory, Institute of Biophysics, Federal Research Centre Krasnoyarsk Scientific Centre, Siberian Branch, Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Department of Biophysics, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodniy Ave, Krasnoyarsk, 660041, Russian Federation
Laboratory of Ecosystem Biophysics, Institute of Biophysics, Federal Research Centre Krasnoyarsk Scientific Centre, Siberian Branch, Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Lopatina, T. S.; Aleksandrova, Y. V.; Anishchenko, O. V.; Gribovskaya, I. V.; Oskina, N. A.; Zotina, T. A.; Zadereev, E. S.

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15.

РУБ Biology + Ecology
Рубрики:
DAPHNIA-MAGNA
   NACL SALINITY
   ROAD SALTS
   WATER
   FRESH
   CHLORIDE
Кл.слова (ненормированные):
salinity -- toxicity test -- Cladocera -- Allium-test -- aquatic ecosystems
Аннотация: Chloride salts are the most commonly used deicing materials for winter maintenance of roads. Numerous studies indicate a significant increase in the salinity of aquatic ecosystems associated with the long-term use of deicing materials in countries located in cold climates. The functioning of ecosystems largely depends on salinity, since salinity is one of the key factors determining the species composition, the structure of food webs and the productivity of aquatic communities. Given the growing threat of salinization of groundwater and surface waters, it is extremely important to study the effect of deicing materials on the biota and functioning of aquatic ecosystems. The aim of this research is to determine the threshold concentrations of solutions of the deicing salt mixture "Bionord" containing sodium and calcium chlorides, at which negative effects on the development of animal and plant test objects are observed. In this study, we used the salt-containing mixture "Bionord" as a model deicer. Similarly, with the most commonly used ice melting chemicals, the "Bionord" salt mixture contains a large amount of sodium and calcium chlorides (about 85% of the total weight). To evaluate the toxicity of the deicer solutions, we used acute and chronic toxicity tests with cladoceran Moina macrocopa (Straus, 1820) (Cladocera: Moinidae) and standard onion-based test with Allium cepa L. (Liliopsida: Amaryllidaceae) (Allium-test). In acute and chronic toxicity tests with Cladocera, the females on the first day of their life (body size 0.5-0.6 mm) were placed individually in jars with aged (not less than for 72 h) tap water with a volume of 20 ml with the addition of a deicer at a certain concentration. A group of animals that was placed in the medium without the deicer was used as a control. In the acute toxicity tests, we used the following concentration of the deicer: 1.3; 2.5; 4.0, 5.0; 6.0; 8.0; 10.0 g/l. The mortality of animals was recorded 24 and 48 hours after the start of the experiment. The concentration of the deicer (LC50) at which 50% of animals was observed to die, compared to the control, was determined in the acute toxicity test. In a chronic toxicity test, animals were tested in the following range of concentrations of the deicer: 0.3; 0.6; 1.3; 2.5; 5.0; 6.0 and 8.0 g/l. The chronic toxicity test was conducted until the death of all test animals. Based on the data obtained in the chronic toxicity test, the specific growth rate of juvenile females, average fecundity, and average life span of M. macrocopa were calculated for each concentration of the deicer. Bulbs of onions of the Stuttgartenrisen variety with a diameter of 1.8 +/- 0.1 cm and a weight of 2.27 +/- 0.17 g were used in the onion test. Bulbs with their bottoms were placed in test tubes containing 20 ml of a solute of the deicer or tap water for 48 hours. Three bulbs were tested for each concentration and for the control. The following concentrations of the deicer were used in the onion test: 1.0; 2.5; 5.0; 7.0; 10.0; 15.0; 20.0; 50.0 g/l. The general toxic and cytotoxic effects were evaluated in the onion test. The average root length and the total root length on each bulb were used as indicators of the total toxicity of the solutions of deicer. To evaluate proliferative activity, we calculated the mitotic index as the fraction of dividing cells in the apical root meristem to the total number of cells. Based on the results of the experiments, we determined median effective mixture concentrations (EC50) at which there is a 50% decrease, compared to the control, in the values of root growth indicators: average root length, sum of root lengths on each bulb and mitotic index. Median lethal concentration (LC50) of the deicing salt determined in the 48-hour acute toxicity test with females of Al. macrocopa was equal to 5.1 g/l. In the chronic test, we showed that the exposure to the solutions of the deicing salt in the range of concentrations from 0.3 to 5.0 g/l does not affect the life span, specific growth rate of juveniles and fecundity of females of M. macrocopa. The median effective concentration (EC50) of the deicing salt determined in the Allium-tests were 6.3, 5.2 and 10.4 g/l for the sum of root lengths, average root length on each bulb and proliferative activity at the tips of roots (mitotic index), respectively (See Table 1 and 2). Complete inhibition of onion root growth was observed at the concentration of the decider equal to 20 g/l, while the death of all test animals in the acute toxicity test occurred at the concentration of the deicer equal to 8,0 g/l (See Fig. 1). Thus, we demonstrated that similar concentrations of the deicer induced 50% inhibition of the growth of onion roots and 50% mortality of cladocerans. These values, in general, corresponded to a critical salinity of 5-8 %o above which qualitative changes occur both in the external and internal condition of aquatic animals. The electrical conductivity of the deicer solutions, which had a negative effect on the selected test species, coincides with the previously obtained values of the electrical conductivity of sodium chloride solutions harmful to cladocerans. We can assume that the main mechanism of the effect of the deicing material that we study is associated with the biological effect of its chlorine and sodium salts Taking this into account, the value of electrical conductivity measured for solutions of deicing salt can be used to assess its negative potential effects. We estimated that in the absence of timely cleaning, regulated by the rules for using the material, the runoff from each square meter of the treated surface can lead to the pollution of 8-13 liters of fresh water. Thus, the basic requirement for the use of deicing salts on roads is the need to comply with the cleaning regime of the treated surfaces. Otherwise, the gradual accumulation of sodium and calcium chlorides in water bodies can cause an increase in salinity which will affect the survival of freshwater aquatic organisms and lead to serious disturbances in the functioning of aquatic ecosystems.

WOS
Держатели документа:
Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Sci Ctr,Lab Ecosyst B, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Sci Ctr,Lab Biolumine, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Sci Ctr,Analyt Lab, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Dept Biophys, 79 Svobodniy Ave, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Lopatina, Tatiana S.; Aleksandrova, Yuliyana, V; Anishchenko, Olesya, V; Gribovskaya, Iliada, V; Oskina, Nataliya A.; Zotina, Tatiana A.; Zadereev, Egor S.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk Krai; Krasnoyarsk Krai Fund for Supporting Scientific and Scientific-Technical Activities [19-44-240014]

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16.

Man as a component of a closed ecological life support system / J. I. Gitelson, U - Okladnikov YuN // Life support & biosphere science : international journal of earth space. - 1994. - Vol. 1, Is. 2. - P73-81 . - ISSN 1069-9422
Кл.слова (ненормированные):
carbon dioxide -- oxygen -- article -- blood analysis -- blood cell count -- breathing -- construction work and architectural phenomena -- crop -- diet -- growth, development and aging -- human -- metabolism -- microclimate -- physiology -- Russian Federation -- space flight -- standard -- waste management -- water supply -- Blood Cell Count -- Blood Chemical Analysis -- Carbon Dioxide -- Crops, Agricultural -- Diet -- Ecological Systems, Closed -- Environment, Controlled -- Facility Design and Construction -- Humans -- Life Support Systems -- Oxygen -- Respiration -- Russia -- Space Flight -- Waste Management -- Water Supply
Аннотация: Material support of all manned space flights so far has been provided from a prestored stock of substances or replenished from the Earth's biosphere. Exploration of space will, however, become real only when man is able to break away from Earth completely, when he will be accompanied by a system providing everything necessary to sustain full-valued life for an unlimited time. The only known system to date meeting this requirement is the Earth's biosphere. To break away from his cradle, as K.E. Tsiolkovsky called Earth, it is necessary to devise a life support system functionally similar to the natural biosphere. This need not be similar in structure to the vast diversity of trophic relationships available on Earth, but requires the solution of a multitude of various problems of an ecological, physiological, engineering and social-psychological nature. Human life-support systems based on biological regeneration of environments in small volumes have been studied at the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) over many years. This work has resulted in the design of Bios-3, a biologically-based self-sustained human life support system.

Scopus
Держатели документа:
Institute of Biophysics (Siberian Branch of Russian Academy of Sciences), Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; , U - Okladnikov YuN

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17.

A mathematical model of "plants-microorganisms" interaction on complete mineral medium and under nitrogen limitation / T. I. Pisman [et al.] // Advances in Space Research. - 1999. - Vol. 24, Is. 3. - P383-387 . - ISSN 0273-1177
Кл.слова (ненормированные):
nitrogen -- ecological modeling -- interspecific interaction -- nutrient limitation -- plant -- rhizosphere -- article -- biological model -- biomass -- comparative study -- culture medium -- drug effect -- growth, development and aging -- mathematics -- microbiology -- plant root -- Pseudomonas fluorescens -- sweating -- wheat -- Biomass -- Culture Media -- Mathematics -- Models, Biological -- Nitrogen -- Plant Roots -- Plant Transpiration -- Pseudomonas fluorescens -- Triticum
Аннотация: A mathematical model concerning the interaction of plants and rhizospheric microorganisms on complete mineral medium and under nitrogen limitation has been constructed. The model takes into account the closeness of plants and microorganisms in terms of the matter released by the plant and consumed by the microorganisms. The effect of rhizospheric microorganisms on plant growth with normal carbon dioxide and complete mineral medium has been demonstrated. Plants interacting with microorganisms have a greater biomass than plants growing without microorganisms. Wheat growth stimulation by metabolites of rhizospheric microorganisms under laboratory conditions on artificial soil has been experimentally demonstrated (Pechurkin, 1997). Under nitrogen limitation , the biomass of plants, with or without microorganisms, is identical, and is substantially reduced as compared with the medium with standard nitrogen. В© 1999 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics (Siberian Branch, Russian Academy of Sciences), Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Pechurkin, N.S.; Mariasova, T.S.; Somova, L.A.; Sarangova, A.B.

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18.

Bioluminescent immunoassay of thyrotropin and thyroxine using obelin as a label [Text] / L. A. Frank, A. I. Petunin, E. S. Vysotski // Anal. Biochem. - 2004. - Vol. 325, Is. 2. - P240-246, DOI 10.1016/j.ab.2003.11.003. - Cited References: 16 . - ISSN 0003-2697

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Chemistry, Analytical
Рубрики:
AEQUORIN
   PURIFICATION
   PROTEIN
   CDNA
Кл.слова (ненормированные):
obelin -- human thyrotropin -- thyroxine -- immunoassay -- bioluminescence
Аннотация: Solid-phase bioluminescent immunoassay of thyroid hormones, human thyrotropin (hTSH) and two forms of thyroxine (T4), whose determinations are vitally important for diagnostics of thyroid diseases and the efficiency of treatment, is described. The recombinant obelin, a Ca2+-regulated photoprotein originally derived from the luminous marine hydroid Obelia longissima, is employed as a bioluminescent label. To produce obelin conjugates with anti-hTSH, anti-T4 immunoglobulins (IgG), and T4, additional SH groups are introduced into the obelin molecule using Traut's reagent (2-iminothiolane) and then obelin possessing extra SH groups is conjugated with succinimidyl 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate-activated IgGs or T4. The total yield of obelin conjugates determined by luminescent activity is 60-65% after all chemical and purification procedures. The obtained conjugates are stable to lyophilization and in solution for at least 9 months at 4 degreesC, with loss of activity not exceeding 10%. The application of obelin conjugates for determination of the hTSH, total T4, and free T4 in standard, control, and patient sera displays high sensitivity and reproducibility of results. The results of bioluminescent immunoassays are closely comparable to those obtained by the radioimmunoassay method (R = 0.95-0.99). (C) 2003 Elsevier Inc. All rights reserved.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
DIAS Ltd, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Petunin, A.I.; Vysotski, E.S.

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19.

Perspectives of different type biological life support systems (BLSS) usage in space missions / S. I. Bartsev [et al.] // Acta Astronautica. - 1996. - Vol. 39, Is. 8. - P617-622, DOI 10.1016/S0094-5765(97)00012-X . - ISSN 0094-5765
Кл.слова (ненормированные):
article -- bacterium -- comparative study -- construction work and architectural phenomena -- environmental planning -- green alga -- human -- instrumentation -- mathematics -- microclimate -- moon -- plant -- quality of life -- space flight -- standard -- weightlessness -- Algae, Green -- Bacteria -- Ecological Systems, Closed -- Environment Design -- Environment, Controlled -- Facility Design and Construction -- Humans -- Life Support Systems -- Mathematics -- Moon -- Plants -- Quality of Life -- Space Flight -- Weightlessness -- Biology -- Life support systems (spacecraft) -- Spreadsheets -- Biological life support systems (BLSS) -- Lunar missions
Аннотация: In the paper an attempt is made to combine three important criteria of LSS comparison: minimum mass, maximum safety and maximum quality of life. Well-known types of BLSS were considered: with higher plant, higher plants and mushrooms, microalgae, and hydrogen-oxidizing bacteria. These BLSSs were compared in terms of "integrated" mass for the case of a vegetarian diet and a "normal" one (with animal proteins and fats). It was shown that the BLSS with higher plants and incineration of wastes becomes the best when the exploitation period is more than 1 yr. The dependence of higher plants' LSS structure on operation time was found. Comparison of BLSSs in terms of integral reliability (this criterion includes mass and quality of life criteria) for a lunar base scenario showed that BLSSs with higher plants are advantageous in reliability and comfort. This comparison was made for achieved level of technology of closing and for perspective one. В© 1997 Elsevier Science Ltd.

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Держатели документа:
Biophys. Inst. of SB RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bartsev, S.I.; Gitelson, J.I.; Lisovsky, G.M.; Mezhevikin, V.V.; Okhonin, V.A.

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20.

A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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