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A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - 9. - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P. 915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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A database on natural and transgenic luminous microorganisms: BiolumBase / S. E. Medvedeva [et al.] // Microbiology. - 2005. - Vol. 74, Is. 2. - P236-243, DOI 10.1007/s11021-005-0057-0 . - ISSN 0026-2617
Кл.слова (ненормированные):
Bioluminescence -- Database -- Natural and transgenic luminous microorganisms -- Bacteria (microorganisms)
Аннотация: The database BiolumBase was designed for the collection and systematization of available information on microorganisms containing bioluminescent systems; it includes two sections: natural and transgenic luminous microorganisms. By now, logic schemes of these sections have been developed, classification of the objects has been performed, ways of presentation of characteristics and structure of fields for input of information have been elaborated, and the necessary program modules have been developed. The database is filled on the basis of published data and our own experimental results; subsequent linkage of the database to the Internet is envisaged. Users will be able to obtain not only catalogues of strains but also information concerning the properties and functions of the known species of luminous bacteria, the structure, regulatory mechanisms, and application of bioluminescent systems and genetically engineered constructions with lux genes, as well as to find references and to search strains by using any set of attributes. The database will provide information that is of interest for the development of microbial ecology and biotechnology, in particular, for the prediction of biological hazard from the application of transgenic strains. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Boyandin, A.N.; Lankin, Yu.P.; Kotov, D.A.; Kargatova, T.V.; Rodicheva, E.K.; Popova, L.Yu.

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A general one-dimensional vertical ecosystem model of Lake Shira (Russia, Khakasia): Description, parametrization and analysis / I. G. Prokopkin [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P585-618, DOI 10.1007/s10452-010-9326-8 . - ISSN 1386-2588
Кл.слова (ненормированные):
Meromictic brackish lake -- One-dimensional vertical ecological model -- Sensitivity analysis -- alga -- biomass -- brackish water -- diffusion -- ecosystem modeling -- meromictic lake -- microbial community -- model test -- nutrient -- one-dimensional modeling -- parameterization -- phytoplankton -- seasonality -- sensitivity analysis -- solar radiation -- vertical profile -- water chemistry -- water temperature -- zooplankton -- Khakassia -- Lake Shira -- Russian Federation -- algae
Аннотация: A one-dimensional ecological model of the meromictic brackish Lake Shira (Russia, Khakasia) was developed. The model incorporates state-of-the-art knowledge about the functioning of the lake ecosystem using the most recent field observations and ideas from PCLake, a general ecosystem model of shallow freshwater lakes. The model of Lake Shira presented here takes into account the vertical dynamics of biomasses of the main species of algae, zooplankton and microbial community, as well as the dynamics of oxygen, detritus, nutrients and hydrogen sulphide from spring to autumn. Solar radiation, temperature and diffusion are modelled using real meteorological data. The parameters of the model were calibrated to the field data, after applying different methods of sensitivity analysis to the model. The resulting patterns of phytoplankton and nutrients dynamics show a good qualitative and quantitative agreement with the field observations during the whole summer season. Results are less satisfactory with respect to the vertical distribution of zooplankton biomass. We hypothesize that this is due to the fact that the current model does not take the sex and age structure of zooplankton into account. The dynamics of oxygen, hydrogen sulphide and the modelled positions of the chemocline and thermocline are again in good agreement with field data. This resemblance confirms the validity of the approach we took in the model regarding the main physical, chemical and ecological processes. This general model opens the way for checking various hypotheses on the functioning of the Lake Shira ecosystem in future investigations and for analysing options for management of this economically important lake. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Biophysics, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Netherlands Institute of Ecology, Centre for Limnology, Rijksstraatweg 6, 3631 AC Nieuwersluis, Netherlands
Netherlands Environmental Assessment Agency (PBL), Postbus 303, 3720 AH Bilthoven, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Prokopkin, I.G.; Mooij, W.M.; Janse, J.H.; Degermendzhy, A.G.

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A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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A new composite material based on alumina nanofibers and detonation nanodiamonds: synthesis, characterization, and sensing application / N. O. Ronzhin, E. D. Posokhina, E. V. Mikhlina [et al.] // J. Nanopart. Res. - 2021. - Vol. 23, Is. 9. - Ст. 199, DOI 10.1007/s11051-021-05309-y. - Cited References:57. - This work is partially supported by the Russian Foundation for Basic Research, Project 18-29-19078 (E. V. Mikhlina, M. M. Simunin, I. Ryzhkov). . - ISSN 1388-0764. - ISSN 1572-896X

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
ELECTROCHEMICAL ENERGY-STORAGE
SELECTIVE DETECTION
PHENOL DETECTION
Кл.слова (ненормированные):
Nanodiamonds -- Alumina nanofibers -- Composite -- Indicator system -- Phenol
Аннотация: The development of inexpensive, easy-to-produce, and easy-to-use analytical tools for detection of harmful and toxic substances is a relevant research problem with direct applications in environmental monitoring and protection. In this work, we propose a novel composite material based on alumina nanofibers and detonation nanodiamonds for detection of phenol in aqueous medium. The composite material was obtained by mixing an aqueous suspension of alumina nanofibers with a diameter of 10-15 nm and a length of several microns and a hydrosol of nanodiamonds with an average cluster size of 70 nm. The mechanisms underlying the interaction of these nanomaterials are clarified and the physicochemical properties of the composite are investigated. The SEM and TEM studies show that the obtained composite has a network structure, in which clusters of nanodiamonds (10-20 nm in diameter) are distributed over the surface of nanofibers. Coupling of nanomaterials occurs due to opposite signs of their zeta potentials, which results in electrostatic attraction and subsequent chemical bonding as indicated by the X-ray photoelectron spectroscopy and simultaneous thermal analysis. The bonding apparently occurs between functional groups (mainly carboxyl) on the surface of nanodiamonds and amphoteric hydroxyl groups on the surface of alumina nanofibers. The proposed composite allows an easy-to-perform colorimetric analysis for qualitative and quantitative determination of phenol in aqueous samples with linear response over a wide range of concentrations (0.5-106 mu M). Multiple tests have shown that the composite is reusable and retains its catalytic function for at least 1 year during storage at room temperature.

WOS
Держатели документа:
Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny 79, Krasnoyarsk 660041, Russia.
Inst Computat Modelling SB RAS, Akademgorodok 50-44, Krasnoyarsk 660036, Russia.
Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia.
Fed Res Ctr KSC SB RAS, Akademgorodok 50-38, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ronzhin, Nikita O.; Posokhina, Ekaterina D.; Mikhlina, Elena, V; Mikhlin, Yuri L.; Simunin, Mikhail M.; Tarasova, Lyudmila S.; Vorobyev, Sergey A.; Bondar, Vladimir S.; Ryzhkov, Ilya I.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078]

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A novel ATP-dependent bioluminescent system from the Siberian earthworm Fridericia heliota: structure elucidation of luciferin and its analogs [Text] / V. . Petushkov [et al.] // Luminescence. - 2014. - Vol. 29. - P54-55. - Cited References: 3 . - ISSN 1522-7235. - ISSN 1522-7243

WOS
Держатели документа:
[Dubinnyi, Maxim
Tsarkova, Aleksandra
Baranov, Mikhail
Yampolsky, Ilia] Russian Acad Sci, Inst Bioorgan Chem, Moscow, Russia
[Petushkov, Valentin
Rodionova, Natalja
Shimomura, Osamu] Russian Acad Sci, Inst Biophys, Lab Photobiol, Siberian Branch, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V...; Dubinnyi, M...; Tsarkova, A...; Rodionova, N...; Baranov, M...; Shimomura, O...; Yampolsky, I...

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A Novel Type of Luciferin from the Siberian Luminous Earthworm Fridericia heliota: Structure Elucidation by Spectral Studies and Total Synthesis [Text] / V. N. Petushkov [et al.] // Angew. Chem.-Int. Edit. - 2014. - Vol. 53, Is. 22. - P5566-5568, DOI 10.1002/anie.201400529. - Cited References: 13. - We thank Dr. Alexander O. Chizhov for recording mass spectra and Dr. K. S. Mineev for NMR analysis of synthetic intermediates. We acknowledge support from the Program of the Government of the Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant no. 11. G34.31.0058), the programs MCB RAS, President of the Russian Federation "Leading science school" (grant 3951.2012.4) and the Russian Foundation for Basic Research (grant 14-03-01015). B. M. S. was supported by a stipend from the Program of the President of the Russian Federation. . - ISSN 1433-7851. - ISSN 1521-3773

РУБ Chemistry, Multidisciplinary
Рубрики:
BIOLUMINESCENT EARTHWORM
Кл.слова (ненормированные):
bioluminescence -- luciferin -- natural products -- NMR spectroscopy -- total synthesis
Аннотация: The structure elucidation and synthesis of the luciferin from the recently discovered luminous earthworm Fridericia heliota is reported. This luciferin is a key component of a novel ATP-dependent bioluminescence system. UV, fluorescence, NMR, and HRMS spectroscopy studies were performed on 0.005 mg of the isolated substance and revealed four isomeric structures that conform to spectral data. These isomers were chemically synthesized and one of them was found to produce light when reacted with a protein extract from F. heliota. The novel luciferin was found to have an unusual extensively modified peptidic nature, thus implying an unprecedented mechanism of action.

WOS
Держатели документа:
[Petushkov, Valentin N.
Rodionova, Natalja S.
Shimomura, Osamu] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia
[Petushkov, Valentin N.
Rodionova, Natalja S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Lab Photobiol, Krasnoyarsk 660036, Russia
[Dubinnyi, Maxim A.
Tsarkova, Aleksandra S.
Baranov, Mikhail S.
Kublitski, Vadim S.
Yampolsky, Ilia V.] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117997, Russia
[Shimomura, Osamu] Marine Biol Lab, Woods Hole, MA 02543 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Dubinnyi, M.A.; Tsarkova, A.S.; Rodionova, N.S.; Baranov, M.S.; Kublitski, V.S.; Shimomura, O...; Yampolsky, I.V.; Government of the Russian Federation "Measures to attract leading scientists to Russian educational institutions" [11. G34.31.0058]; programs MCB RAS; Russian Federation "Leading science school" [3951.2012.4]; Russian Foundation for Basic Research [14-03-01015]; Russian Federation

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10.

A one-dimensional model for phytoflagellate distribution in the meromictic lake [Text] / I. G. Prokopkin, Y. V. Barkhatov, E. B. Khromechek // Ecol. Model. - 2014. - Vol. 288. - P. 1-8, DOI 10.1016/j.ecolmode1.2014.05.011. - Cited References: 22. - This work was supported by Russian Foundation for Basic Research (RFBR) No. 13-04-01514 and Integrative Project of Siberian Branch of Russian Academy of Sciences No. 56. The anonymous reviewers are kindly acknowledged for valuable suggestions and criticism. . - ISSN 0304-3800. - ISSN 1872-7026

РУБ Ecology
Рубрики:
VERTICAL-DISTRIBUTION
   CHLOROPHYLL MAXIMUM
   SOUTH SIBERIA
   RUSSIA
   KHAKASIA
   SHIRA
   CRYPTOPHYTES
   CRYPTOMONAS
   CHEMOCLINE
   MIGRATION
Кл.слова (ненормированные):
Phytoflagellates -- Meromictic lake -- Simulation modeling -- Lake Shira
Аннотация: Using mathematical modeling methods, the work investigates possible existence of a phytoflagellate population in the ecosystem of a stratified lake (Lake Shira, Khakasia, Russia). Until this study was carried out, no phytoflagellate population had been detected in the lake, although there had been indirect evidence of the possible presence of a cryptomonad population in this water body. To answer the question if this population may be present in this water body, as the first step of investigation, a mathematical model was developed describing the microorganism biomass dynamics and phytoflagellate relationship with the lake food web. The calculations showed that cryptophytic algae were abundant in the water column layers above the lake chemocline at the beginning of summer but showed low biomass concentrations during other periods of time. In the summer following the construction of the model, samples were collected from Lake Shira, as the next step of the study. A cryptophytic algae population was found in the water column, and the pattern of its distribution in the ecosystem was similar to that predicted by the model. In addition to that, we studied theoretically possible reasons for the early summer development of the population in the deep water layers and found that it was the result of the mixotrophic feeding strategy of cryptomonads and trophic interactions in the food web. The study shows that preliminary modeling of the locational and temporal distribution of populations whose presence in the ecosystem is only suggested by indirect evidence may be useful as a tool for searching for the species in natural ecosystems and as an approach enabling a more accurate description of the food web structure of a water body. (C) 2014 Elsevier B.V. All rights reserved.

WOS
Держатели документа:
[Prokopkin, I. G.
Barkhatov, Y. V.
Khromechek, E. B.] Akademgorodok, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Prokopkin, I.G.; Barkhatov, Y.V.; Khromechek, E.B.; Russian Foundation for Basic Research (RFBR) [13-04-01514]; Integrative Project of Siberian Branch of Russian Academy of Sciences [56]

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11.

A one-dimensional model of vertical stratification of Lake Shira focussed on winter conditions and ice cover / S. N. Genova [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P571-584, DOI 10.1007/s10452-010-9327-7 . - ISSN 1386-2588
Кл.слова (ненормированные):
Convective mixed layer -- Ice formation -- Ice melting -- Simplified model -- Winter profiles -- anoxic conditions -- hydrogen sulfide -- ice cover -- inhomogeneity -- meromictic lake -- numerical model -- one-dimensional modeling -- pycnocline -- salinity -- seasonality -- stratification -- vertical profile -- water column -- water temperature -- Khakassia -- Lake Shira -- Russian Federation
Аннотация: In meromictic lakes such as Lake Shira, horizontal inhomogeneity is small in comparison with vertical gradients. To determine the vertical distribution of temperature, salinity, and density of water in a deep zone of a Lake Shira, or other saline lakes, a one-dimensional (in vertical direction) mathematical model is presented. A special feature of this model is that it takes into account the process of ice formation. The model of ice formation is based on the one-phase Stefan problem with the linear temperature distribution in the solid phase. A convective mixed layer is formed under an ice cover due to salt extraction in the ice formation process. To obtain analytical solutions for the vertical distribution of temperature, salinity, and density of water, we use a scheme of vertical structure in the form of several layers. In spring, the ice melts as top and bottom. These processes are taken into account in the model. The calculated profiles of salinity and temperature of Shira Lake are in good agreement with field measurement data for each season. Additionally, we focussed on the redox zone, which is the zone in which the aerobic layers of a water column meet the anaerobic ones. Hyperactivity of plankton communities is observed in this zone in lakes with hydrogen sulphide monimolimnion, and Lake Shira is among them. The location of the redox zone in the lake, which is estimated from field measurements, coincides with a sharp increase in density (the pycnocline) during autumn and winter. During spring and summer, the redox zone is deeper than the pycnocline. The location of pycnocline calculated with the hydro physical model is in good agreement with field measurement data. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Computational Modeling SB RAS, Akademgorodok 50/44, Krasnoyarsk 660036, Russian Federation
Krasnoyarsk, Siberian Federal University, Institute of Mathematics, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Federal University, Institute of Mathematics, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Netherlands Institute of Ecology, Center for Limnology, Rijksstraatweg 6, Nieuwersluis 3631 AC, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Genova, S.N.; Belolipetskii, V.M.; Rogozin, D.Y.; Degermendzhy, A.G.; Mooij, W.M.

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12.

A study of forest vegetation dynamics in the south of the Krasnoyarskii Krai in spring / M. Chernetskiy [et al.] // Advances in Space Research. - 2011. - Vol. 48, Is. 5. - P819-825, DOI 10.1016/j.asr.2011.04.032 . - ISSN 0273-1177
Кл.слова (ненормированные):
EVI -- Forestry -- MODIS -- NDVI -- Remote sensing -- Vegetation phenology -- Accurate measurement -- Annual time series -- Carbon exchange -- Data series -- Dynamic state -- Enhanced vegetation index -- EVI -- Forest vegetation -- Global scale -- Growth dynamics -- Interannual variability -- Moderate resolution imaging spectroradiometer -- MODIS -- NDVI -- Normalized difference vegetation index -- Principal components analysis -- Remote sensing applications -- Remote sensing data -- Satellite data -- Spatial structure -- Spring season -- Terrestrial ecosystems -- Vegetation dynamics -- Vegetation phenology -- Biology -- Climate models -- Dynamics -- Ecosystems -- Estimation -- Forestry -- Monitoring -- Principal component analysis -- Radiometers -- Remote sensing -- Satellite imagery -- Timber -- Time series -- User interfaces -- Vegetation -- Carbon -- Ecosystems -- Forests -- Image Analysis -- Plants -- Remote Sensing -- Time Series Analysis
Аннотация: Remote sensing applications have greatly enhanced ability to monitor and manage in the areas of forestry. Accurate measurements of regional and global scale vegetation dynamics (phenology) are required to improve models and understanding of inter-annual variability in terrestrial ecosystem carbon exchange and climate-biosphere interactions. Study of vegetation phenology is required for understanding of variability in ecosystem. In this paper, monitoring of vegetation dynamics using time series of satellite data is presented. Vegetation variability (vegetation rate) in different topoclimatic areas is investigated. Original software using IDL interactive language for processing of satellite long-term data series was developed. To investigate growth dynamics vegetation rate inferred from remote sensing was used. All estimations based on annual time series of Moderate Resolution Imaging Spectroradiometer (MODIS) imagery. Vegetation rate for Enhanced Vegetation Index (EVI) and Normalized Difference Vegetation Index (NDVI) was calculated using MODIS data. The time series covers spring seasons of each of 9 years, from 2000 to 2008. Comparison of EVI and NDVI derived growth rates has shown that NDVI derived rates reveal spatial structure better. Using long-term data of vegetation rates variance was estimated that helps to reveal areas with anomalous growth rate. Such estimation shows sensitivity degree of different areas to different topoclimatic conditions. Woods of heights depend on spatial topoclimatic variability unlike woods of lowlands. Principal components analysis shows vegetation with different rate conditions. Also it reveals vegetation of same type in areas with different conditions. It was demonstrated that using of methods for estimating the dynamic state of vegetation based on remote sensing data enables successful monitoring of vegetation phenology. В© 2011 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Chernetskiy, M.; Pasko, I.; Shevyrnogov, A.; Slyusar, N.; Khodyayev, A.

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13.

All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2 +-loaded apo-berovin / L. P. Burakova [et al.] // J. Photochem. Photobiol. B Biol. - 2016. - Vol. 154. - P57-66, DOI 10.1016/j.jphotobiol.2015.11.012 . - ISSN 1011-1344
Кл.слова (ненормированные):
Aequorin -- Bioluminescence -- Calcium -- Coelenterazine -- Obelin
Аннотация: Light-sensitive photoprotein berovin accounts for a bright bioluminescence of ctenophore Beroe abyssicola. Berovin is functionally identical to the well-studied Ca2+-regulated photoproteins of jellyfish, however in contrast to those it is extremely sensitive to the visible light. Berovin contains three EF-hand Ca2+-binding sites and consequently belongs to a large family of the EF-hand Ca2+-binding proteins. Here we report the spatial structure of apo-berovin with bound Mg2+ determined at 1.75 A. The magnesium ion is found in each functional EF-hand loop of a photoprotein and coordinated by oxygen atoms donated by the side-chain groups of aspartate, carbonyl groups of the peptide backbone, or hydroxyl group of serine with characteristic oxygen-Mg2+ distances. As oxygen supplied by the side-chain of the twelfth residue of all Ca2+-binding loops participates in the magnesium ion coordination, it was suggested that Ca2+-binding loops of berovin belong to the mixed Ca2+/Mg2+ rather than Ca2+-specific type. In addition, we report an effect of physiological concentration of Mg2+ on bioluminescence of berovin (sensitivity to Ca2+, rapid-mixed kinetics, light-sensitivity, thermostability, and apo-berovin conversion into active protein). The different impact of physiological concentration of Mg2+ on berovin bioluminescence as compared to hydromedusan photoproteins was attributed to different affinities of the Ca2 +-binding sites of these photoproteins to Mg2+. © 2015 Elsevier B.V. All rights reserved.

Scopus,
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Держатели документа:
Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok 50, Krasnoyarsk, Russian Federation
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing, China
IHuman Institute, ShanghaiTech University, 99 Haike Road, Shanghai, China

Доп.точки доступа:
Burakova, L. P.; Natashin, P. V.; Malikova, N. P.; Niu, F.; Pu, M.; Vysotski, E. S.; Liu, Z.-J.
Свободных экз. нет
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14.

alpha-C-Mannosyltryptophan is a Structural Analog of the Luciferin from Bioluminescent Siberian Earthworm Henlea sp. / M. A. Dubinnyi, I. A. Ivanov, N. S. Rodionova [et al.] // ChemistrySelect. - 2020. - Vol. 5, Is. 42. - P13155-13159, DOI 10.1002/slct.202003075. - Cited References:49. - This work was supported by the State Assignment for Basic Research of the Russian Academy of Sciences (project no. 0356-2019-0019) and the Russian Foundation for Basic Research (project no. 19-04-00348-a). . - ISSN 2365-6549

РУБ Chemistry, Multidisciplinary
Рубрики:
STRUCTURE ELUCIDATION
   MANNOSYLATION
   TRYPTOPHAN
   PROTEIN
   COMPLEMENT
Кл.слова (ненормированные):
Bioluminescence -- Earthworm -- Henlea -- Natural products -- NMR spectroscopy
Аннотация: Cold extract from bioluminescent earthworm Henlea sp. was studied by HPLC, 1D and 2D NMR and LC-HRMS analysis. An abundant structural analog of the luciferin was isolated and identified as alpha-C-mannosyltryptophan (ManTrp), the product of unusual C2-glycosylation found earlier in humans, ascidians and other animals. Two compounds in cold extract (P300b, P300c) were characterized as C2-substituted derivatives of tryptophan. We hypothesize that a series of tryptophan-containing compounds are possible participants of bioluminescence-related metabolism in Henlea sp.

WOS
Держатели документа:
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, GSP-7,Miklukho Maklaya Str 16-10, Moscow 117997, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Pirogov Russian Natl Res Med Univ, 1 Ostrovityanova St, Moscow 117997, Russia.

Доп.точки доступа:
Dubinnyi, Maxim A.; Ivanov, Igor A.; Rodionova, Natalia S.; Kovalchuk, Sergey I.; Kaskova, Zinaida M.; Petushkov, Valentin N.; State Assignment for Basic Research of the Russian Academy of Sciences [0356-2019-0019]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00348-a]

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15.

Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics / T. G. Volova [et al.] // Polym Test. - 2018. - Vol. 65. - P54-68, DOI 10.1016/j.polymertesting.2017.10.023 . - ISSN 0142-9418
Кл.слова (ненормированные):
Antibacterial activity -- Antibiotics -- Bacterial cellulose -- Composites -- Properties -- Silver nanoparticles -- Antibiotics -- Atoms -- Boron carbide -- Cell culture -- Cellulose -- Cellulose films -- Composite materials -- Escherichia coli -- Materials testing apparatus -- Metal nanoparticles -- Nanocomposite films -- Nanoparticles -- Scanning electron microscopy -- Silver compounds -- Spectrum analysis -- Synthesis (chemical) -- Tensile testing -- Water pollution -- X ray analysis -- Anti-bacterial activity -- Antibacterial properties -- Bacterial cellulose -- Mechanical characteristics -- Properties -- Silver nanoparticles -- Structure and properties -- Tensile testing machines -- Silver -- Antibiotics -- Cellulose -- Composites -- Properties -- Silver
Аннотация: The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO3: as AgNO3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones. © 2017 Elsevier Ltd

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Смотреть статью,
WOS
Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 43/50 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Shumilova, A. A.; Shidlovskiy, I. P.; Nikolaeva, E. D.; Sukovatiy, A. G.; Vasiliev, A. D.; Shishatskaya, E. I.

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16.

Architectonics of a mixed colony Flavobacterium sp 56+Bacillus cereus 60 [Текст] / A. P. Puzyr, O. A. Mogil'naya, E. L. Pavlyuchenko // Izv. Akad. Nauk Ser. Biol. - 1999. - Is. 3. - P. 378-384. - Cited References: 15 . - ISSN 0002-3329

РУБ Biology

Аннотация: Electron microscopy has shown that the colonies obtained as a result of joint growth of bacterial monocultures Flavobacterium sp. 56 and Bacillus cereus 60 consist of three main cell types. Two cell types of the mixed colonies preserve the fine structure specific for bacteria in the monocultures. At the same time, formation of a large amount of the third type of cells is stimulated, which occur as single cells in the monoculture Flavobacterium sp. 56. The cell types are not intermixed in the colony volume and have certain spatial positions.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr, A.P.; Mogil'naya, O.A.; Pavlyuchenko, E.L.

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17.

AsLn2, a luciferin-related modified tripeptide from the bioluminescent earthworm Fridericia heliota / V. N. Petushkov [et al.] // Tetrahedron Letters. - 2014. - Vol. 55, Is. 2. - P463-465, DOI 10.1016/j.tetlet.2013.11.061 . - ISSN 0040-4039
Кл.слова (ненормированные):
Bioluminescence -- Fridericia heliota -- Luciferin -- Modified peptide
Аннотация: AsLn2, an unusual modified peptide, was isolated from the bioluminescent earthworm Fridericia heliota (Enchytraeidae). Its structure, elucidated by NMR and mass spectrometry, includes residues of tyrosine, CompX (a novel tyrosine modification product, reported in the accompanying paper), and N(omega)-acylated lysine. Chromatography, UV, and 1H NMR data imply a close structural similarity of AsLn2 with F. heliota luciferin. AsLn2 appears to be an intermediate or by-product in F. heliota luciferin biosynthesis. © 2013 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, pr. Svobodnyi, 79, Krasnoyarsk 660041, Russian Federation
Laboratory of Photobiology, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation
Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Campo Alegre St. 687, Porto 4169-007, Portugal
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Dubinnyi, M.A.; Rodionova, N.S.; Nadezhdin, K.D.; Marques, S.M.; Esteves Da Silva, J.C.G.; Shimomura, O.; Yampolsky, I.V.

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18.

Assessment of the efficacy of slow-release formulations of the tribenuron-methyl herbicide in field-grown spring wheat / T. G. Volova, N. L. Kurachenko, V. L. Bopp [et al.] // Environ. Sci. Pollut. Res. - 2021, DOI 10.1007/s11356-021-17195-x. - Cited References:72. - The work on production and investigation of polymer films was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation [Grant No. 074-02-2018-328]. . - Article in press. - ISSN 0944-1344. - ISSN 1614-7499

РУБ Environmental Sciences
Рубрики:
BIODEGRADABLE POLY-3-HYDROXYBUTYRATE
WILD MUSTARD
Кл.слова (ненормированные):
Tribenuron-methyl -- P(3HB) -- Slow-release formulations -- Spring wheat -- Weed -- control -- Yield structure -- Grain quality
Аннотация: The efficacy of slow-release formulations of tribenuron-methyl (TBM) embedded in the matrix of degradable poly(3-hydroxybutyrate) blended with birch wood flour [polymer/wood flour/herbicide 50/30/20 wt.%] was compared with the efficacy of TBM as the active ingredient of the Mortira commercial formulation, which was applied as post-emergence spray to treat spring wheat cv. Novosibirskaya 15. The study was conducted in Central Siberia (in the environs of the city of Krasnoyarsk, Russia) from May to August 2020. The biological efficacy of the embedded TBM was 92.3%, which was considerably higher than the biological efficacy of the Mortira formulation used as the post-emergence spray (15.4%). The embedding of TBM into degradable blended matrix enabled long-duration functioning of this unstable herbicide in soil. The sensitivity of weed plants to TBM differed depending on the species. TBM was more effective against A. retroflexus and A. blitoides, which were killed at an earlier stage, than against C. album and G. aparine, whose percentage increased in the earlier stage and which were controlled by the herbicide less effectively and at later stages. On the plot treated with the embedded herbicide, the parameters of the wheat yield structure were the best, and the total yield was the highest: 3360 +/- 40 kg/ha versus 3250 +/- 50 kg/ha in the group of plants sprayed with the Mortira formulation. The grain produced in all groups was of high quality and was classified as Grade 1 food grain. The highest quality parameters (grain hectoliter mass, gluten, and protein contents) were obtained in the group of plants treated with the embedded herbicide. The study of the embedded TBM confirmed the high efficacy of the experimental formulation.

WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Ave, Krasnoyarsk 660041, Russia.
RAS, SB, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk State Agrarian Univ, 90 Mir Ave, Krasnoyarsk 660049, Russia.
Mahatma Gandhi Univ, Int & Inter Univ Ctr Nanosci & Nanotechnol, Kottayam 686560, Kerala, India.

Доп.точки доступа:
Volova, Tatiana G.; Kurachenko, Natalya L.; Bopp, Valentina L.; Thomas, Sabu; Demidenko, Aleksey V.; Kiselev, Evgeniy G.; Baranovsky, Sergey V.; Sukovatyi, Aleksey G.; Zhila, Natalia O.; Shishatskaya, Ekaterina I.; Ministry of Education and Science of the Russian FederationMinistry of Education and Science, Russian Federation [074-02-2018-328]

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19.

Assessment of the possibility of establishing material cycling in an experimental model of the bio-technical life support system with plant and human wastes included in mass exchange / A. A. Tikhomirov [et al.] // Acta Astronautica. - 2011. - Vol. 68, Is. 9-10. - P1548-1554, DOI 10.1016/j.actaastro.2010.10.005 . - ISSN 0094-5765
Кл.слова (ненормированные):
Biological-technical life support system -- Photosynthesizing unit -- Utilization of plant and human wastes -- Biological substrates -- Chemical component -- Experimental models -- Human waste -- Life support systems -- Mass exchange -- Mass transfer process -- Material cycling -- Photosynthesizing unit -- Physicochemical methods -- Pilot model -- Plant biomass -- Plant wastes -- Recycled products -- Salicornia europaea -- Simultaneous use -- Soil-like substrate -- Utilization of plant and human wastes -- Sodium chloride -- Substrates -- Waste incineration -- Waste utilization
Аннотация: A pilot model of a bio-technical life support system (BTLSS) including human and plant wastes has been developed at the Institute of Biophysics SB RAS (Krasnoyarsk, Russia). This paper describes the structure of the photosynthesizing unit of the system, which includes wheat, chufa and vegetables. The study substantiates the simultaneous use of neutral and biological substrates for cultivating plants. A novel physicochemical method for the involvement of human wastes in the cycling has been employed, which enables the use of recycled products as nutrients for plants. Inedible plant biomass was subjected to biological combustion in the soil-like substrate (SLS) and was thus involved in the system mass exchange; NaCl contained in native urine was returned to the human through the consumption of Salicornia europaea, an edible salt-concentrating plant. Mass transfer processes in the studied BLSS have been examined for different chemical components. В© 2009 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
SB RAS Institute of Biophysics, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation
Blaise Pascal University, France
ESA-ESTEC, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Velichko, V.V.; Tikhomirova, N.A.; Kudenko, Y.A.; Gribovskaya, I.V.; Gros, J.-B.; Lasseur, C.

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20.

Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine [Text] / Z. J. Liu [et al.] // Biochem. Biophys. Res. Commun. - 2003. - Vol. 311, Is. 2. - P433-439, DOI 10.1016/j.bbrc.2003.09.231. - Cited References: 29 . - ISSN 0006-291X

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CRYSTAL-STRUCTURE
   BIOLUMINESCENT PROTEIN
   VIOLET BIOLUMINESCENCE
   PHOTOPROTEIN AEQUORIN
   ANGSTROM RESOLUTION
   RECOMBINANT OBELIN
   W92F OBELIN
   PURIFICATION
   REFINEMENT
   EXPRESSION
Кл.слова (ненормированные):
photoprotein -- bioluminescence -- atomic resolution -- EF-hand
Аннотация: The spatial structure of the Ca2+-regulated photoprotein obelin has been solved to resolution of 1.1 Angstrom. Two oxygen atoms are revealed substituted at the C2-position of the coelenterazine in contrast to the obelin structure at 1.73 Angstrom resolution where one oxygen atom only was disclosed. The electron density of the second oxygen atom was very weak but after exposing the crystals to a trace of Ca2+, the electron densities of both oxygen atoms became equally intense. In addition, one Ca2+ was found bound in the loop of the first EF-hand motif. Four of the ligands were provided by protein residues Asp30, Asn32, Asn34, and the main chain oxygen of Lys36. The other two were from water molecules. From a comparison of B-factors for the residues constituting the active site, it is suggested that the variable electron densities observed in various photoprotein structures could be attributed to different mobilities of the peroxy oxygen atoms. (C) 2003 Elsevier Inc. All rights reserved.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Univ Georgia, Dept Chem, Athens, GA 30602 USA
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Liu, Z.J.; Vysotski, E.S.; Deng, L...; Lee, J...; Rose, J...; Wang, B.C.

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