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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kiselev, Evgeniy G., Boyandin, Anatoly N., Zhila, Natalia O., Prudnikova, Svetlana, V, Shumilova, Anna A., Baranovskiy, Sergey, V, Shishatskaya, Ekaterina, I, Thomas, Sabu, Volova, Tatiana G.
Заглавие : Constructing sustained-release herbicide formulations based on poly-3-hydroxybutyrate and natural materials as a degradable matrix
Колич.характеристики :14 с
Коллективы : Government of the Russian Federation [074-02-2018-328, 220]
Место публикации : Pest Manag. Sci.: JOHN WILEY & SONS LTD. - ISSN 1526-498X, DOI 10.1002/ps.5702. - ISSN 1526-4998(eISSN)
Примечания : Cited References:83. - This study was financially supported by the project 'Agropreparations of the new generation: a strategy of construction and realization' (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, 'On measures designed to attract leading scientists to the Russian institutions of higher learning'.
Предметные рубрики: SOIL MICROBIAL COMMUNITY
FENOXAPROP-P-ETHYL
SLOW-RELEASE
METRIBUZIN
Аннотация: BACKGROUND The purpose of the present study was to develop ecofriendly herbicide formulations. Its main aim was to develop and investigate slow-release formulations of herbicides (metribuzin, tribenuron-methyl, and fenoxaprop-P-ethyl) of different structure, solubility, and specificity, which were loaded into a degradable matrix of poly-3-hydroxybutyrate (P(3HB)) blended with available natural materials (peat, clay, and wood flour). RESULTS Differences in the structure and physicochemical properties of the formulations were studied depending on the type of the matrix. Herbicide release and accumulation in soil were associated with the solubility of the herbicide. Fourier-transform infrared spectroscopy showed that no chemical bonds were formed between the components in the experimental formulations. Degradation of the formulations in agro-transformed soil in laboratory conditions was chiefly influenced by the shape of the specimens (granules or pellets) while the effect of the type of filler (peat, clay, or wood flour) was insignificant. The use of granules enabled more rapid accumulation of the herbicides in soil: their peak concentrations were reached after 3 weeks of incubation while the concentrations of the herbicides released from the pellets were the highest after 5-7 weeks. Loading of the herbicides into the polymer matrix composed of the slowly degraded P(3HB) and natural materials enabled both sustained function of the formulations in soil (lasting between 1.5 and = 3 months) and stable activity of the otherwise rapidly inactivated herbicides such as tribenuron-methyl and fenoxaprop-P-ethyl. CONCLUSION The experimental herbicide formulations enabled slow release of the active ingredients to soil. (c) 2019 Society of Chemical Industry
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovalev V. S., Grandl W., Manukovsky N. S., Tikhomirov A. A., Bock C.
Заглавие : Modeling a lunar base mushroom farm
Колич.характеристики :6 с
Место публикации : Life Sci. Space Res.: ELSEVIER, 2022. - Vol. 33. - С. 1-6. - ISSN 2214-5524, DOI 10.1016/j.lssr.2021.12.005. - ISSN 2214-5532(eISSN)
Примечания : Cited References:31
Предметные рубрики: SUPPORT
CULTIVATION
Аннотация: To calculate the equivalent system mass of mushrooms, a conceptual configuration of a mushroom farm as part of a bioregenerative life support system on an inhabited lunar base was designed. The mushroom farm consists of two connected modules. Each module is a double-shell rigid pipe-in-pipe aluminum structure. The first module is used to prepare and sterilize the substrate, while the mushrooms are sown and grown in the second module. Planned productivity of the mushroom farm is 28 kg of fresh mushrooms per one process cycle lasting 66 days for 14 consumers. Mushroom production can be increased using additional modules. The calculated equivalent system masses of the mushroom farm and the mushrooms produced therein is 88,432 kg and 31,550 kg per 1 kg of dry mushrooms in one process cycle, respectively. At that, the biggest contributor to the equivalent system mass of mushrooms is the total pressurized volume of the farm - 68%. The results obtained may be a prerequisite for performing trade-off studies between different configurations of mushroom farm and calculating a space diet using the equivalent system mass of mushrooms.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G. G., Prudnikova, Svetlana V. V., Kiselev, Evgeniy G. G., Nemtsev, Ivan V. V., Vasiliev, Alexander D. D., Kuzmin, Andrey P. P., Shishatskaya, Ekaterina I. I.
Заглавие : Bacterial Cellulose (BC) and BC Composites: Production and Properties
Колич.характеристики :24 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Nanomaterials: MDPI, 2022. - Vol. 12, Is. 2. - Ст.192. - ISSN 2079-4991(eISSN), DOI 10.3390/nano12020192
Примечания : Cited References:113. - This research was financially supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006.
Предметные рубрики: SILVER NANOPARTICLES
GLUCONACETOBACTER-HANSENII
MICROBIAL CELLULOSE
Аннотация: The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m(2) and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khromechek, Elena B., Barkhatov, Yuri, V, Rogozin, Denis Y.
Заглавие : Community structure and vertical distribution of planktonic ciliates in the saline meromictic lake Shira during breakdown of meromixis
Колич.характеристики :11 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR); Krasnoyarsk Krai Government; Krasnoyarsk Regional Fund of Science [19-44-240002]; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [19-05-00428]
Место публикации : Ecohydrol. Hydrobiol.: INT CENTRE ECOLOGY, POLISH ACAD SCIENCES, 2021. - Vol. 21, Is. 1. - С. 142-152. - ISSN 1642-3593, DOI 10.1016/j.ecohyd.2020.08.001. - ISSN 2080-3397(eISSN)
Примечания : Cited References:41. - The authors are grateful to F.F. Kozlov, V.V. Zykov, and A.P. Tolomeev for their assistance during field studies. The present study was funded by RFBR and Krasnoyarsk Krai Government and the Krasnoyarsk Regional Fund of Science, project number 19-44-240002. The present study was financially supported by Russian Foundation for Basic Research (RFBR) Project No. 19-05-00428.
Предметные рубрики: SHUNET SOUTH SIBERIA
SEASONAL SUCCESSION
PROTOZOA
FOOD
Аннотация: The study deals with the vertical distribution and seasonal dynamics of planktonic ciliates in the pelagic and littoral zones of a saline meromictic Lake Shira. Fourteen species of free-living ciliates have been found in the Lake, seven of them inhabiting the pelagic zone. The richness of ciliates is higher both in terms of the number of species and biomass in the littoral zone compared to the pelagic zone. Although the ciliate species diversity is low in the pelagic zone, in certain seasons, the biomass of some of the species may reach considerable values, up to 3.4 g m(-2) in the water column. The biomass of ciliates in Lake Shira is generally comparable to the average values for other mesotrophic lakes. The abundance and composition of ciliate populations in Lake Shira vary considerably with depth. Changes in the Lake ecosystem caused by meromixis breakdown in 2015-2016, which induced considerable variations in many Lake components, affected the vertical distribution of planktonic ciliates. However, the annual average biomass of the species that inhabited the Lake before meromixis breakdown remained unchanged. The total ciliate biomass increased due to the presence of the new species. (C) 2021 European Regional Centre for Ecohydrology of the Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorokhovatsky A. Y., Chepurnykh T. V., Shcheglov A. S., Mokrushina Y. A., Baranova M. N., Goncharuk S. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 52-55. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010051
Аннотация: Abstract: A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. © 2021, The Author(s),.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh T. V., Kotlobay A. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010026
Аннотация: Abstract: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination. © 2021, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh, T., V, Kotlobay A. A., Purtov, K., V, Petushkov V. N., Rodionova N. S., Yampolsky, I., V
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Колич.характеристики :4 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052P]; President grant for leading scientific schoolsLeading Scientific Schools Program [NSh-2605.2020.4]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 1607-6729, DOI 10.1134/S1607672921010026. - ISSN 1608-3091(eISSN)
Примечания : Cited References:12. - This work was supported by the grant from the Russian Science Foundation no. 16-14-00052P, alanine screening was performed by the President grant for leading scientific schools NSh-2605.2020.4.
Аннотация: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tomilin F. N., Rogova A. V., Burakova L. P., Tchaikovskaya O. N., Avramov P. V., Fedorov D. G., Vysotski E. S.
Заглавие : Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory
Место публикации : Photochem. Photobiol. Sci.: Springer Nature, 2021. - Vol. 20, Is. 4. - С. 559-570. - ISSN 1474905X (ISSN), DOI 10.1007/s43630-021-00039-5
Аннотация: Active hydromedusan and ctenophore Ca2+-regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound 2-hydroperoxycoelenterazine (an oxygenated intermediate of coelenterazine). Whereas the absorption maximum of hydromedusan photoproteins is at 460–470 nm, ctenophore photoproteins absorb at 437 nm. Finding out a physical reason for this blue shift is the main objective of this work, and, to achieve it, the whole structure of the protein–substrate complex was optimized using a linear scaling quantum–mechanical method. Electronic excitations pertinent to the spectra of the 2-hydroperoxy adduct of coelenterazine were simulated with time-dependent density functional theory. The dihedral angle of 60° of the 6-(p-hydroxy)-phenyl group relative to the imidazopyrazinone core of 2-hydroperoxycoelenterazine molecule was found to be the key factor determining the absorption of ctenophore photoproteins at 437 nm. The residues relevant to binding of the substrate and its adopting the particular rotation were also identified. © 2021, The Author(s), under exclusive licence to European Photochemistry Association,European Society for Photobiology.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tomilin, Felix N., Rogova, Anastasia V., Burakova, Ludmila P., Tchaikovskaya, Olga N., Avramov, Pavel V., Fedorov, Dmitri G., Vysotski, Eugene S.
Заглавие : Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory
Колич.характеристики :12 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [20-04-00085]; NSFCNational Natural Science Foundation of China (NSFC) [19-54-53004]; Russian Ministry of Science and EducationMinistry of Education and Science, Russian Federation [0721-2020-0033]
Место публикации : Photochem. Photobiol. Sci.: SPRINGERNATURE, 2021. - Vol. 20, Is. 4. - С. 559-570. - ISSN 1474-905X, DOI 10.1007/s43630-021-00039-5. - ISSN 1474-9092(eISSN)
Примечания : Cited References:61. - The ab initio quantum chemical calculations were funded by RFBR and NSFC as the research project No. 19-54-53004 and RFBR research project No. 20-04-00085. The development of structural atomistic model of berovin without calcium ions generated by the I-TASSER server was funded by project 0721-2020-0033 of the Russian Ministry of Science and Education.
Аннотация: Active hydromedusan and ctenophore Ca2+-regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound 2-hydroperoxycoelenterazine (an oxygenated intermediate of coelenterazine). Whereas the absorption maximum of hydromedusan photoproteins is at 460-470 nm, ctenophore photoproteins absorb at 437 nm. Finding out a physical reason for this blue shift is the main objective of this work, and, to achieve it, the whole structure of the protein-substrate complex was optimized using a linear scaling quantum-mechanical method. Electronic excitations pertinent to the spectra of the 2-hydroperoxy adduct of coelenterazine were simulated with time-dependent density functional theory. The dihedral angle of 60 degrees of the 6-(p-hydroxy)-phenyl group relative to the imidazopyrazinone core of 2-hydroperoxycoelenterazine molecule was found to be the key factor determining the absorption of ctenophore photoproteins at 437 nm. The residues relevant to binding of the substrate and its adopting the particular rotation were also identified.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorokhovatsky, A. Yu, Chepurnykh, T., V, Shcheglov A. S., Mokrushina, Yu A., Baranova M. N., Goncharuk S. A., Purtov, K., V, Petushkov V. N., Rodionova N. S., Yampolsky, I., V
Заглавие : The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties
Колич.характеристики :4 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052-P]; President's grant for state support of the leading scientific schools of the Russian Federation [NSh-2605.2020.4]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 52-55. - ISSN 1607-6729, DOI 10.1134/S1607672921010051. - ISSN 1608-3091(eISSN)
Примечания : Cited References:10. - The work was supported by the Russian Science Foundation (project no. 16-14-00052-P). The creation of the luciferase-producing yeast strain nnLuz was supported by the President's grant for state support of the leading scientific schools of the Russian Federation (NSh-2605.2020.4).
Аннотация: A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova, Irina Yu, Karpowicz, Maciej, Gladyshev, Michail I., Sushchik, Nadezhda N., Petrosyan, Varos G., Sakharova, Ekaterina G., Dzialowski, Andrew R.
Заглавие : Effects of Macrobiota on the Transfer Efficiency of Essential Elements and Fatty Acids From Phytoplankton to Zooplankton Under Eutrophic Conditions
Колич.характеристики :15 с
Коллективы : Polish National Science Centre [2016/21/B/NZ8/00434]; Russian Science FoundationRussian Science Foundation (RSF) [21-14-00123]; Federal Tasks for Institute of Biophysics SB RAS [51.1.1]; Federal Tasks for Siberian Federal University [FSRG-2020-0019]; Polish National Agency for Academic ExchangePolish National Agency for Academic Exchange (NAWA) [PPN/ULM/2020/1/00258/U/DRAFT/00001]
Место публикации : Front. Environ. Sci.: FRONTIERS MEDIA SA, 2021. - Vol. 9. - Ст.739014. - ISSN 2296-665X(eISSN), DOI 10.3389/fenvs.2021.739014
Примечания : Cited References:76. - This experiment was performed with support from the Polish National Science Centre (2016/21/B/NZ8/00434). The statistical analysis and its interpretation was performed with support from the Russian Science Foundation (Grant No. 21-14-00123). Biochemical analyses were performed with support by Federal Tasks for Institute of Biophysics SB RAS No. 51.1.1 and Federal Tasks for Siberian Federal University No. FSRG-2020-0019. The preparation of the manuscript by Feniova I. was supported by the Polish National Agency for Academic Exchange (Agreement No. PPN/ULM/2020/1/00258/U/DRAFT/00001).
Предметные рубрики: FRESH-WATER ZOOPLANKTON
DAPHNIA-LONGISPINA
ZEBRA MUSSELS
Аннотация: The transfer pathways of organic matter and elements from phytoplankton to zooplankton in freshwater ecosystems are important for understanding how aquatic ecosystems function. We conducted a mesocosm experiment to determine how fish and zebra mussels altered the transfer efficiencies of essential substances including carbon (C), polyunsaturated fatty acids (PUFAs), total fatty acids (FAs), phosphorus (P), and nitrogen (N) from phytoplankton to zooplankton. We assessed the transfer efficiencies of the essential substances from phytoplankton to zooplankton as the ratio of their zooplankton production (P) per unit of biomass (B) to that of phytoplankton to exclude grazing or predation effects. We hypothesized that zebra mussels and fish would affect the transfer of materials from phytoplankton to zooplankton by altering the contents of essential elements and FAs in phytoplankton and zooplankton communities and/or due to shifts in the planktonic community structure mediated by grazing and/or predation. Fish increased the transfer efficiencies of eicosapentaenoic acid 20:5 omega-3 (EPA), docosahexaenoic acid 22:6 omega-3 (DHA), and P relative to the control. We speculated that fish weakened the control of zooplankton over algal assemblage by selectively feeding on larger cladocerans such as Daphnia. Therefore, fish can increase the relative proportion of high-quality food for zooplankton, improving food conditions for the available zooplankton. In contrast, zebra mussels reduced the transfer efficiencies of EPA and DHA relative to the control treatment likely due to competition with zooplankton for PUFA-rich food particles. However, zebra mussels did not have any impact on the transfer efficiencies of C, total FAs, N, and P. EPA, DHA, and P were transferred more efficiently than C from phytoplankton to zooplankton, while total FAs, which are commonly used as an energetic source, were transferred as efficiently as C. The enrichment of consumers with the most important substances relative to their basal food sources creates the potential for the successful transport of these substances across aquatic trophic webs./p
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova, Marina D., Wu, Lijie, Eremeeva, Elena, V, Natashin, Pavel, V, Gulnov, Dmitry, V, Nemtseva, Elena, V, Liu, Dongsheng, Liu, Zhi-Jie, Vysotski, Eugene S.
Заглавие : Crystal structure of semisynthetic obelin-v
Колич.характеристики :16 с
Коллективы : National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [32011530076]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00085, 20-44-240006, 20-54-53011]
Место публикации : Protein Sci.: WILEY, 2021. - Article in press. - ISSN 0961-8368, DOI 10.1002/pro.4244. - ISSN 1469-896X(eISSN)
Примечания : Cited References:69. - National Natural Science Foundation of China, Grant/Award Number: 32011530076; Russian Foundation for Basic Research, Grant/Award Numbers: 20-04-00085, 20-44-240006, 20-54-53011
Предметные рубрики: CA2+-REGULATED PHOTOPROTEIN OBELIN
PHOTOLUMINESCENCE QUANTUM YIELD
Аннотация: Coelenterazine-v (CTZ-v), a synthetic derivative with an additional benzyl ring, yields a bright bioluminescence of Renilla luciferase and its "yellow" mutant with a significant shift in the emission spectrum toward longer wavelengths, which makes it the substrate of choice for deep tissue imaging. Although Ca2+-regulated photoproteins activated with CTZ-v also display red-shifted light emission, in contrast to Renilla luciferase their bioluminescence activities are very low, which makes photoproteins activated by CTZ-v unusable for calcium imaging. Here, we report the crystal structure of Ca2+-regulated photoprotein obelin with 2-hydroperoxycoelenterazine-v (obelin-v) at 1.80 angstrom resolution. The structures of obelin-v and obelin bound with native CTZ revealed almost no difference; only the minor rearrangement in hydrogen-bond pattern and slightly increased distances between key active site residues and some atoms of 2-hydroperoxycoelenterazine-v were found. The fluorescence quantum yield (phi(FL)) of obelin bound with coelenteramide-v (0.24) turned out to be even higher than that of obelin with native coelenteramide (0.19). Since both obelins are in effect the enzyme-substrate complexes containing the 2-hydroperoxy adduct of CTZ-v or CTZ, we reasonably assume the chemical reaction mechanisms and the yields of the reaction products (phi(R)) to be similar for both obelins. Based on these findings we suggest that low bioluminescence activity of obelin-v is caused by the low efficiency of generating an electronic excited state (phi(S)). In turn, the low phi(S) value as compared to that of native CTZ might be the result of small changes in the substrate microenvironment in the obelin-v active site.
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13.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Boyandin A. N., Sukhanova A. A., Nikolaeva E. D., Sukovatyi A. G., Nemtsev I. V.
Заглавие : Blends of poly-3-hydroxybutyrate and poly-ε-caprolactone: Morphology, properties and biocompatibility
Место публикации : AIP Conference Proceedings: American Institute of Physics Inc., 2021. - Vol. 2388: 4th International Conference on Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020 (16 November 2020 through 20 November 2020, ) Conference code: 174383. - Ст.020002. - , DOI 10.1063/5.0068677
Аннотация: A series of films was obtained from blends of poly-3-hydroxybutyrate (PHB) and poly-s-caprolactone (PCL). The structure, physical, mechanical and biological properties of the films were studied, depending on the ratio of the components. The microscopic structure of mixed films was determined by the main component of the blend: the films were porous with a predominance of PHB and relatively smooth with a predominance of PCL. The highest values of elongation at break (229-269%), as well as the local maximum values of tensile strength (14-15 MPa) were noted for blends with 5-25% PHB content. At PHB content of 25% and 75%, local maxima of Young's modulus were observed (507 and 655 MPa, respectively). When the ratio of the components in the blend was equal (50:50 by weight), the films were heterogeneous and had the lowest values of mechanical strength (ultimate strength and elongation at break were 3 MPa and 15.5%, respectively). A study of the biocompatibility of mixed films in a culture of NIH 3T3 mouse fibroblasts based on determination of the number of adhered cells in the MTT test showed the absence of cytotoxic action, as well as high adhesion and cell proliferation, in some cases exceeding the data for films from pure PHB and PCL. This demonstrates the suitability of PHB-PCL blends for the production of cell carriers for tissue engineering and other reconstructive technologies. © 2021 Author(s).
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Kurachenko, Natalya L., Bopp, Valentina L., Thomas, Sabu, Demidenko, Aleksey V., Kiselev, Evgeniy G., Baranovsky, Sergey V., Sukovatyi, Aleksey G., Zhila, Natalia O., Shishatskaya, Ekaterina I.
Заглавие : Assessment of the efficacy of slow-release formulations of the tribenuron-methyl herbicide in field-grown spring wheat
Колич.характеристики :16 с
Коллективы : Ministry of Education and Science of the Russian FederationMinistry of Education and Science, Russian Federation [074-02-2018-328]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2021. - Article in press. - ISSN 0944-1344, DOI 10.1007/s11356-021-17195-x. - ISSN 1614-7499(eISSN)
Примечания : Cited References:72. - The work on production and investigation of polymer films was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation [Grant No. 074-02-2018-328].
Предметные рубрики: BIODEGRADABLE POLY-3-HYDROXYBUTYRATE
WILD MUSTARD
Аннотация: The efficacy of slow-release formulations of tribenuron-methyl (TBM) embedded in the matrix of degradable poly(3-hydroxybutyrate) blended with birch wood flour [polymer/wood flour/herbicide 50/30/20 wt.%] was compared with the efficacy of TBM as the active ingredient of the Mortira commercial formulation, which was applied as post-emergence spray to treat spring wheat cv. Novosibirskaya 15. The study was conducted in Central Siberia (in the environs of the city of Krasnoyarsk, Russia) from May to August 2020. The biological efficacy of the embedded TBM was 92.3%, which was considerably higher than the biological efficacy of the Mortira formulation used as the post-emergence spray (15.4%). The embedding of TBM into degradable blended matrix enabled long-duration functioning of this unstable herbicide in soil. The sensitivity of weed plants to TBM differed depending on the species. TBM was more effective against A. retroflexus and A. blitoides, which were killed at an earlier stage, than against C. album and G. aparine, whose percentage increased in the earlier stage and which were controlled by the herbicide less effectively and at later stages. On the plot treated with the embedded herbicide, the parameters of the wheat yield structure were the best, and the total yield was the highest: 3360 +/- 40 kg/ha versus 3250 +/- 50 kg/ha in the group of plants sprayed with the Mortira formulation. The grain produced in all groups was of high quality and was classified as Grade 1 food grain. The highest quality parameters (grain hectoliter mass, gluten, and protein contents) were obtained in the group of plants treated with the embedded herbicide. The study of the embedded TBM confirmed the high efficacy of the experimental formulation.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Nemtsev I., Lukyanenko A., Vasiliev A., Kiselev E., Sukovatyi A., Volova T.
Заглавие : Polymer Films of Poly-3-hydroxybutyrate Synthesized by Cupriavidus necator from Different Carbon Sources
Место публикации : J. Polym. Environ.: Springer, 2021. - Vol. 29, Is. 3. - С. 837-850. - ISSN 15662543 (ISSN), DOI 10.1007/s10924-020-01924-3
Аннотация: Films were prepared from 2% solutions of biodegradable poly-3-hydroxybutyrate [P(3HB)] and investigated. The polymer was synthesized by the Cupriavidus necator B-10646 bacterium cultivated using various carbon sources (glucose and glycerol of different degrees of purity, containing 0.3 to 17.93% impurities). Glycerol as the substrate influenced molecular-weight properties and crystallinity of the polymer without affecting its temperature characteristics. The P(3HB) specimens synthesized from glycerol had reduced Mw (300–400 kDa) and degree of crystallinity (50–55%) compared to the specimens synthesized from glucose (860 kDa and 76%, respectively). The low-crystallinity P(3HB) specimens, regardless of the degree of purity of glycerol, produced a beneficial effect on the properties of polymer films, which had a better developed folded surface and increased hydrophilicity. The values of the highest roughness (Ra) of the films synthesized from glycerol were 1.8 to 4.0 times lower and the water angles 1.4–1.6 times smaller compared to the films synthesized from glucose (71.75 nm and 87.4°, respectively). Those films performed better as cell scaffolds: the number of viable NIH fibroblasts was 1.7–1.9 times higher than on polystyrene (control) or films of P(3HB) synthesized from glucose. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova, Svetlana, V, Evgrafova, Svetlana Yu, Volova, Tatiana G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
Колич.характеристики :12 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Chemosphere: PERGAMON-ELSEVIER SCIENCE LTD, 2021. - Vol. 263. - Ст.128180. - ISSN 0045-6535, DOI 10.1016/j.chemosphere.2020.128180. - ISSN 1879-1298(eISSN)
Примечания : Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia).
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lisitsa, Albert E., Sukovatyi, Lev A., Bartsev, Sergey, I, Deeva, Anna A., Kratasyuk, Valentina A., Nemtseva, Elena, V
Заглавие : Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction
Колич.характеристики :19 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science [20-44-243002]; RFBRRussian Foundation for Basic Research (RFBR) [20-34-90118]
Место публикации : Int. J. Mol. Sci.: MDPI, 2021. - Vol. 22, Is. 16. - Ст.8827. - ISSN 1422-0067(eISSN), DOI 10.3390/ijms22168827
Примечания : Cited References:59. - The research was funded by the Ministry of Science and Higher Education of the Russian Federation (projects No. FSRZ-2020-0006); by RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (project No. 20-44-243002); by RFBR according to the research project No. 20-34-90118.
Предметные рубрики: FLAVIN INTERMEDIATE
REDUCED FLAVIN
RATE CONSTANTS
LUCIFERASE
Аннотация: Enzymes activity in a cell is determined by many factors, among which viscosity of the microenvironment plays a significant role. Various cosolvents can imitate intracellular conditions in vitro, allowing to reduce a combination of different regulatory effects. The aim of the study was to analyze the media viscosity effects on the rate constants of the separate stages of the bacterial bioluminescent reaction. Non-steady-state reaction kinetics in glycerol and sucrose solutions was measured by stopped-flow technique and analyzed with a mathematical model developed in accordance with the sequence of reaction stages. Molecular dynamics methods were applied to reveal the effects of cosolvents on luciferase structure. We observed both in glycerol and in sucrose media that the stages of luciferase binding with flavin and aldehyde, in contrast to oxygen, are diffusion-limited. Moreover, unlike glycerol, sucrose solutions enhanced the rate of an electronically excited intermediate formation. The MD simulations showed that, in comparison with sucrose, glycerol molecules could penetrate the active-site gorge, but sucrose solutions caused a conformational change of functionally important alpha Glu175 of luciferase. Therefore, both cosolvents induce diffusion limitation of substrates binding. However, in sucrose media, increasing enzyme catalytic constant neutralizes viscosity effects. The activating effect of sucrose can be attributed to its exclusion from the catalytic gorge of luciferase and promotion of the formation of the active site structure favorable for the catalysis.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ronzhin, Nikita O., Posokhina, Ekaterina D., Mikhlina, Elena, V, Mikhlin, Yuri L., Simunin, Mikhail M., Tarasova, Lyudmila S., Vorobyev, Sergey A., Bondar, Vladimir S., Ryzhkov, Ilya I.
Заглавие : A new composite material based on alumina nanofibers and detonation nanodiamonds: synthesis, characterization, and sensing application
Колич.характеристики :14 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078]
Место публикации : J. Nanopart. Res.: SPRINGER, 2021. - Vol. 23, Is. 9. - Ст.199. - ISSN 1388-0764, DOI 10.1007/s11051-021-05309-y. - ISSN 1572-896X(eISSN)
Примечания : Cited References:57. - This work is partially supported by the Russian Foundation for Basic Research, Project 18-29-19078 (E. V. Mikhlina, M. M. Simunin, I. Ryzhkov).
Предметные рубрики: ELECTROCHEMICAL ENERGY-STORAGE
SELECTIVE DETECTION
PHENOL DETECTION
Аннотация: The development of inexpensive, easy-to-produce, and easy-to-use analytical tools for detection of harmful and toxic substances is a relevant research problem with direct applications in environmental monitoring and protection. In this work, we propose a novel composite material based on alumina nanofibers and detonation nanodiamonds for detection of phenol in aqueous medium. The composite material was obtained by mixing an aqueous suspension of alumina nanofibers with a diameter of 10-15 nm and a length of several microns and a hydrosol of nanodiamonds with an average cluster size of 70 nm. The mechanisms underlying the interaction of these nanomaterials are clarified and the physicochemical properties of the composite are investigated. The SEM and TEM studies show that the obtained composite has a network structure, in which clusters of nanodiamonds (10-20 nm in diameter) are distributed over the surface of nanofibers. Coupling of nanomaterials occurs due to opposite signs of their zeta potentials, which results in electrostatic attraction and subsequent chemical bonding as indicated by the X-ray photoelectron spectroscopy and simultaneous thermal analysis. The bonding apparently occurs between functional groups (mainly carboxyl) on the surface of nanodiamonds and amphoteric hydroxyl groups on the surface of alumina nanofibers. The proposed composite allows an easy-to-perform colorimetric analysis for qualitative and quantitative determination of phenol in aqueous samples with linear response over a wide range of concentrations (0.5-106 mu M). Multiple tests have shown that the composite is reusable and retains its catalytic function for at least 1 year during storage at room temperature.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla, Rachel M., Mette, Elizabeth M., Williamson, Craig E., Adamovich, Boris V., Adrian, Rita, Anneville, Orlane, Balseiro, Esteban, Ban, Syuhei, Chandra, Sudeep, Colom-Montero, William, Devlin, Shawn P., Dix, Margaret A., Dokulil, Martin T., Feldsine, Natalie A., Feuchtmayr, Heidrun, Fogarty, Natalie K., Gaiser, Evelyn E., Girdner, Scott F., Gonzalez, Maria J., Hambright, K. David, Hamilton, David P., Havens, Karl, Hessen, Dag O., Hetzenauer, Harald, Higgins, Scott N., Huttula, Timo H., Huuskonen, Hannu, Isles, Peter D. F., Joehnk, Klaus D., Keller, Wendel Bill, Klug, Jen, Knoll, Lesley B., Korhonen, Johanna, Korovchinsky, Nikolai M., Koster, Oliver, Kraemer, Benjamin M., Leavitt, Peter R., Leoni, Barbara, Lepori, Fabio, Lepskaya, Ekaterina V., Lottig, Noah R., Luger, Martin S., Maberly, Stephen C., MacIntyre, Sally, McBride, Chris, McIntyre, Peter, Melles, Stephanie J., Modenutti, Beatriz, Muller-Navarra L., Pacholski, Laura, Paterson, Andrew M., Pierson, Don C., Pislegina, Helen V., Plisnier, Pierre-Denis, Richardson, David C., Rimmer, Alon, Rogora, Michela, Rogozin, Denis Y., Rusak, James A., Rusanovskaya, Olga O., Sadro, Steve, Salmaso, Nico, Saros, Jasmine E., Sarvala, Jouko, Saulnier-Talbot, Emilie, Schindler, Daniel E., Shimaraeva, Svetlana V., Silow, Eugene A., Sitoki, Lewis M., Sommaruga, Ruben, Straile, Dietmar, Strock, Kristin E., Swain, Hilary, Tallant, Jason M., Thiery, Wim, Timofeyev, Maxim A., Tolomeev, Alexander P., Tominaga, Koji, Vanni, Michael J., Verburg, Piet, Vinebrooke, Rolf D., Wanzenbock, Josef, Weathers, Kathleen, Weyhenmeyer, Gesa A., Zadereev, Egor S., Zhukova, Tatyana V.
Заглавие : Global data set of long-term summertime vertical temperature profiles in 153 lakes
Колич.характеристики :12 с
Коллективы : Belarus Republican Foundation for Fundamental Research; AnaEE-France; SILA (Syndicat Mixte du Lac d'Annecy); Castle Lake Environmental Research and Education Program, University of Nevada at Reno; EC project "Response of European Freshwater Lakes [ENV4-CT97-0453]; EC-project "Climate Impacts on European Lakes" [EVK1-CT-2002-00121]; Austrian Climate and Energy Fund [K09ACK00046]; NSFNational Science Foundation (NSF) [DEB 1950170]; Crater Lake National Park Long-Term Limnological Monitoring Program; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes [UOWX1503]; National Capital Authority; ACT, Australia [GB/NERC/BAS/AEDC/00063]; Belgian Science Policy OfficeBelgian Federal Science Policy Office; North Temperate Lakes LTER NTL-LTER [1440297]; NSERC CanadaNatural Sciences and Engineering Research Council of Canada (NSERC); Canada Research Chairs, Canada Foundation for InnovationCanada Foundation for InnovationCanada Research Chairs; University of Regina; Commissione Internazionale per la protezione delle acque italo-svizzere; Natural Environment Research CouncilUK Research & Innovation (UKRI)Natural Environment Research Council (NERC) [NE/R016429/1]; U.S. NSF Arctic LTER [DEB1637459, LTER_EU_IT_008]; Canton of Zurich [18-44-06201, 20-64-46003]; Russian Ministry of Higher Education and Research [FZZE-2020-0026, FZZE-2020-0023]; National Science Foundation Long Term Research in Environmental Biology program [DEB-1242626]; National Park Service (the Inventory and Monitoring Program); Acadia National Park monitoring program; Gordon and Betty Moore FoundationGordon and Betty Moore Foundation; Andrew Mellon Foundation; US National Science FoundationNational Science Foundation (NSF) [9318452, 9726877, 0235755, 0743192, 1255159]; Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological StationUniversity of Michigan System; Belgian Science Policy Office (BELSPO)Belgian Federal Science Policy Office [CD/AR/02 A]; Waikato Regional Council; NIWA; Swedish Research CouncilSwedish Research CouncilEuropean Commission [2017-00635, NSF DEB 1754276]; Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00362 A, 19-05-00428]
Место публикации : Sci. Data: NATURE PORTFOLIO, 2021. - Vol. 8, Is. 1. - Ст.200. - ISSN 2052-4463(eISSN), DOI 10.1038/s41597-021-00983-y
Примечания : Cited References:45. - This work was conceived at the Global Lake Ecological Observatory Network (GLEON), and benefited from continued participation and travel support from GLEON. This manuscript is dedicated to the late Karl Havens and Alon Rimmer, who provided data for this manuscript. Funding and support for this work came from the following sources: the Belarus Republican Foundation for Fundamental Research; the IGB Long-term Ecological Research Programme; SOERE OLA, AnaEE-France, INRA Thonon les Bains, SILA (Syndicat Mixte du Lac d'Annecy), CISALB (Comite Intercommunautaire pour l'Assainissement du Lac du Bourget), and CIPEL (Commission Internationale pour la protection des eaux du Leman); Shiga Prefectural Fisheries Experiment Station (SPFES); Castle Lake Environmental Research and Education Program, University of Nevada at Reno and UC Davis; the Flathead Lake Monitoring program funded through a consortium of state and private funds, and thank the generous citizens of Flathead Lake for their continued support of lake monitoring; the Institute for water ecology, fish biology and lake research and the Institute for Limnology of the Austrian Academy of Sciences (until 2011), and acknowledge the sampling efforts by many individuals over the long period of investigation, especially H. Gassner, M. Luger, H. Ficker, and R. Kurmayer; the EC project "Response of European Freshwater Lakes to Environmental and Climatic Change" (REFLECT, ENV4-CT97-0453), the EC-project "Climate Impacts on European Lakes" (CLIME, EVK1-CT-2002-00121), the project "Risk Analysis of Direct and Indirect Climate effects on deep Austrian Lake Ecosystems" (RADICAL) funded by the Austrian Climate and Energy Fund (No. K09ACK00046) -Austrian Climate Research Programme (ACRP, http://www.klimafonds.gv.at); O. Garcia and E. Bocel for data analysis and management; D. Cabrera, M.W. Dix, G. Ochaeta, S. van Tuylen, M. Orozco, E. Symonds for sampling efforts; NSF grant No. 0947096 to E. Rejmankova, U.S. PeaceCorps and Ministerio de Ambiente y Recursos Naturales of Guatemala; H. Swain, L. Battoe, K. Main, N. Deyrup (Archbold Biological Station), the Florida Lakewatch program, E. Gaiser (Florida International University); the Crater Lake National Park Long-Term Limnological Monitoring Program; the City of Tulsa (R. West and A. Johnson), the Grand River Dam Authority (R. M. Zamor), W.M. Matthews and US ACE (T. Clyde), and the Oklahoma Water Resources Board; Bay of Plenty Regional Council; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes (UOWX1503); the field and laboratory staff of the South Florida Water Management District for collecting and analyzing the samples; the Norwegian Water Resources and Energy Directorate (NVE), by courtesy of A. S. Kvambekk; the Lake Champlain Long-term Monitoring program (VT DEC and NY DEC); the National Capital Authority, ACT, Australia; Ontario Ministry of Environment, Conservation and Parks; FirstLight Power Resources and Friends of the Lake, especially G. Bollard and R. White; the Finnish Environment Institute SYKE database (Hertta) and S. Mitikka; N. Spinelli and the Lake Wallenpaupack Watershed Management District; Lakes Heywood, Moss, and Sombre: Long-Term Monitoring of Signy Lake Chemistry by BAS 1963-2004. Ref: GB/NERC/BAS/AEDC/00063, and dataset supplied by the Polar Data Centre under Open Government License (c) NERC-BAS, Lake Nkugute: Beadle (1966), CLANIMAE project funded by the Belgian Science Policy Office; Dr. L.; Garibaldi; NSF awards #1418698 and North Temperate Lakes LTER NTL-LTER #1440297; NSERC Canada, Canada Research Chairs, Canada Foundation for Innovation, Province of Saskatchewan, University of Regina, and Queen's University Belfast; Commissione Internazionale per la protezione delle acque italo-svizzere, Ufficio della protezione delle acque e dell'approvvigionamento idrico del Canton Ticino; KamchatNIRO scientists; Natural Environment Research Council award number NE/R016429/1 as part of the UK-SCaPE programme delivering National Capability; U.S. NSF Arctic LTER DEB1637459; Belgian Science Policy (Choltic, Climlake, Climfish); Ontario Ministry of Natural Resources' Harkness Laboratory of Fisheries Research, especially T. Middel; Max-Planck-Institute for Limnology Plon; staff at Erken Laboratory; Mohonk Preserve and D. Smiley; Lake Sunapee Protective Association; KLL database; International Commission for the Protection of Swiss-Italian Waters (CIPAIS) and the LTER (Long Term Ecological Research) Italian network, site "Southern Alpine lakes", LTER_EU_IT_008; staff and students at MECP's Dorset Environmental Science Centre; the LTER (Long-Term Ecological Research) Italian network, site "Southern Alpine lakes", IT08-005-A (http://www.lteritalia.it), with the support of the ARPA Veneto; Prof. L. Chapman, McGill University (Montreal, Quebec, Canada); Amt fur Abfall, Wasser, Energie und Luft (AWEL) of the Canton of Zurich; grants of RSCF project #18-44-06201 and #20-64-46003, of Russian Ministry of Higher Education and Research (projects. FZZE-2020-0026;. FZZE-2020-0023), and of Foundation for support of applied ecological studies "Lake Baikal" (https://baikalfoundation.ru/project/tochka-1/); National Science Foundation Long Term Research in Environmental Biology program (DEB-1242626); the National Park Service (the Inventory and Monitoring Program as well as the Air Resources Division) and Acadia National Park and the Acadia National Park monitoring program; Gordon and Betty Moore Foundation, the Andrew Mellon Foundation, the US National Science Foundation and the Bristol Bay salmon processors; J. Franzoi, G. Larsen, and S. Morales, and the LTSER platform Tyrolean Alps, which belongs to the national and international long-term ecological research network (LTER-Austria, LTER Europe and ILTER); Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological Station (A. Schubel) and Cooperative Institute for Great Lakes Research (R. Miller); the Belgian Science Policy Office (BELSPO) is acknowledged for supporting research on Lake Kivu through the research project EAGLES (CD/AR/02 A); US National Science Foundation awards 9318452, 9726877, 0235755, 0743192 and 1255159; West Coast Regional Council, the Bay of Plenty Regional Council, and Waikato Regional Council, and NIWA; D. Schindler (funding and data access) and B. Parker (logistical support and data management); Swedish Infrastructure for Ecosystem Science (SITES) and the Swedish Research Council under the grant no 2017-00635; NSF DEB 1754276 and NSF DEB 1950170, the Ohio Eminent Scholar in Ecosystem Ecology fund, and Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic Research, grant. 19-04-00362 A and. 19-05-00428.
Предметные рубрики: CLIMATE-CHANGE
THERMAL STRATIFICATION
OXYGEN DEPLETION
FISH
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change.
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